Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| a predominant multidrug-resistant salmonella enterica serovar saintpaul clonal line in german turkey and related food products. | recently, salmonella enterica subsp. enterica serovar saintpaul has increasingly been observed in several countries, including germany. however, the pathogenic potential and epidemiology of this serovar are not very well known. this study describes biological attributes of s. saintpaul isolates obtained from turkeys in germany based on characterization of their pheno- and genotypic properties. fifty-five s. saintpaul isolates from german turkeys and turkey-derived food products isolated from 200 ... | 2010 | 20363784 |
| comparison of genotypes of salmonella enterica serovar enteritidis phage type 30 and 9c strains isolated during three outbreaks associated with raw almonds. | in 2000 to 2001, 2003 to 2004, and 2005 to 2006, three outbreaks of salmonella enterica serovar enteritidis were linked with the consumption of raw almonds. the s. enteritidis strains from these outbreaks had rare phage types (pt), pt30 and pt9c. clinical and environmental s. enteritidis strains were subjected to pulsed-field gel electrophoresis (pfge), multilocus variable-number tandem repeat analysis (mlva), and dna microarray-based comparative genomic indexing (cgi) to evaluate their genetic ... | 2010 | 20363782 |
| application of fourier transform infrared spectroscopy and chemometrics for differentiation of salmonella enterica serovar enteritidis phage types. | fourier transform infrared (ft-ir) spectroscopy and chemometric techniques were used to discriminate five closely related salmonella enterica serotype enteritidis phage types, phage type 1 (pt1), pt1b, pt4b, pt6, and pt6a. intact cells and outer membrane protein (omp) extracts from bacterial cell membranes were subjected to ft-ir analysis in transmittance mode. spectra were collected over a wavenumber range from 4,000 to 600 cm(-1). partial least-squares discriminant analysis (pls-da) was used t ... | 2010 | 20363777 |
| expression of tvia is transiently repressed by hfq in salmonella enterica serovar typhi at hyperosmotic stress. | the putative global post-transcriptional regulator gene hfq was deleted in salmonella enterica serovar typhi (salmonella typhi). genomic dna microarray assay and quantitative real time pcr were used to estimate the level of gene expression. the expression of tvia, the gene required for expression of the vi capsular antigen, was increased in the hfq mutant at 30 min of an up-shift osmotic stress but was not at sustained high or low osmolarity, compared to the wild type strain. in addition, the le ... | 2010 | 20363312 |
| development and validation of a resistance and virulence gene microarray targeting escherichia coli and salmonella enterica. | a microarray was developed to simultaneously screen escherichia coli and salmonella enterica for multiple genetic traits. the final array included 203 60-mer oligonucleotide probes, including 117 for resistance genes, 16 for virulence genes, 25 for replicon markers, and 45 other markers. validity of the array was tested by assessing inter-laboratory agreement among four collaborating groups using a blinded study design. internal validation indicated that the assay was reliable (area under the re ... | 2010 | 20362014 |
| pulsed-field gel electrophoresis associated to phage typing improves the discrimination of epidemiologically unrelated salmonella enterica serovar typhimurium isolates. | a combination of phage typing and pulsed-field gel electrophoresis (pfge) of xbal- and blnl-digested chromosomal dna has been used to study 18 epidemiologically unrelated human salmonella enterica serovar typhimurium isolates, which were collected during 2007 within a single romanian county. phage typing could assign only four of the isolates to three definitive phage types (dt41, dt86, and dt116), the rest being untypable by this classical method. pfge analysis of the double enzyme-digested dna ... | 2009 | 20361529 |
| dna microarray analysis of salmonella serotype typhimurium strains causing different symptoms of disease. | salmonella enterica subsp. enterica is one of the leading food-borne pathogens in the usa and european countries. outcome of human salmonella serotype typhimurium infections ranges from mild self-limiting diarrhoea to severe diarrhoea that requires hospitalization. increased knowledge of the mechanisms that are responsible for causing infection and especially the severity of infection is of high interest. | 2010 | 20356366 |
| inactivation of salmonella enterica and listeria monocytogenes inoculated on hydroponic tomatoes using chlorine dioxide gas. | the main objective of this study was to determine survivability of a cocktail of three strains of salmonella enterica (montevideo, javiana, and baildon) and two strains of listeria monocytogenes (lcdc 81-861 and f4244) on hydroponic tomatoes after treatment with chlorine dioxide (clo(2)) gas. an initial concentration of 8-9 log cfu/ml of salmonella and listeria cocktails was inoculated individually, in separate experiments, on tomato skin to obtain a population of 7-8 log cfu/cm(2) after drying ... | 2010 | 20353288 |
| [construction of a promoter reporter array for antibiotic screening]. | we designed and constructed an antibiotic screening system by using antibiotic responsive genes as reporters. plasmid pcs26 carrying a promoterless luminescence reporter, luxcdabe, was used as the vector and the promoter regions of antibiotic responsive genes/operons from escherichia coli were cloned upstream of the lux reporter to form the first part of the screening reporter array. random promoter library of salmonella enterica and pseudomonas aeruginosa were screened for antibiotic responsive ... | 2010 | 20353098 |
| peptidomic analysis of the skin secretions of the frog pachymedusa dacnicolor. | high-resolution mass spectrometry-based peptidomics has been used to characterize several components in electro-stimulated skin secretions of the endemic mexican frog pachymedusa dacnicolor. peptide mass screening performed in an orbitrap-xl mass spectrometer showed that p. dacnicolor skin secretions possess 194 different components with molecular masses ranging mainly from 500 to 6,000 da. dozens of molecules were partially sequenced including two novel protease inhibitors. additionally, one po ... | 2011 | 20352461 |
| ramr mutations in clinical isolates of klebsiella pneumoniae with reduced susceptibility to tigecycline. | five klebsiella pneumoniae isolates with reduced susceptibility to tigecycline (mic, 2 microg/ml) were analyzed. a gene homologous to ramr of salmonella enterica was identified in klebsiella pneumoniae. sequencing of ramr in the nonsusceptible klebsiella strains revealed deletions, insertions, and point mutations. transformation of mutants with wild-type ramr genes, but not with mutant ramr genes, restored susceptibility to tigecycline and repressed overexpression of rama and acrb. thus, this st ... | 2010 | 20350947 |
| 16s rrna methyltransferase rmtc in salmonella enterica serovar virchow. | we screened salmonella and escherichia coli isolates, collected 2004-2008 in the united kingdom, for 16s rrna methyltransferases. rmtc was identified in s. enterica serovar virchow isolates from clinical samples and food. all isolates were clonally related and bore the rmtc gene on the bacterial chromosome. surveillance for and research on these resistance determinants are essential. | 2010 | 20350396 |
| a soluble form of the pilus protein fima targets the vdac-hexokinase complex at mitochondria to suppress host cell apoptosis. | inhibition of apoptotic response of host cells during an early phase of infection is a strategy used by many enteroinvasive bacterial pathogens to enhance their survival. here, we report the identification of a soluble form of the pilus protein fima from the culture supernatants of e. coli k1, salmonella, and shigella that can potently inhibit bax-mediated release of cytochrome c from isolated mitochondria. similar to the infected cells, hct116 cells stably expressing fima display a delay in the ... | 2010 | 20347420 |
| reversible n epsilon-lysine acetylation regulates the activity of acyl-coa synthetases involved in anaerobic benzoate catabolism in rhodopseudomonas palustris. | rhodopseudomonas palustris grows photoheterotrophically on aromatic compounds available in aquatic environments rich in plant-derived lignin. benzoate degradation is regulated at the transcriptional level in r. palustris in response to anoxia and the presence of benzoate and/or benzoyl-coa (bz-coa). here, we report evidence that anaerobic benzoate catabolism in this bacterium is also regulated at the post-translational level. in this pathway, benzoate is activated to bz-coa by the amp-forming bz ... | 2010 | 20345662 |
| [construction and characterization of recombinant salmonella enterica serovar choleraesuis vaccine strain expressing heterologous antigen of pasteurella multocida toxin]. | this study was aimed to construct and characterize salmonella vaccine strain c500 expressing the recombinant pasteurella multocida toxin (pmt) antigen by the asd+ balanced-lethal host-vector system. | 2010 | 20344946 |
| genetic analysis of multidrug-resistant salmonella enterica serovars stanley and typhimurium from cattle. | during 2005-2008, a longitudinal study was conducted in southern japan to detect and characterize multidrug-resistant salmonella enterica serovars recovered from cattle diagnostic specimens. determination of antimicrobial resistance phenotypes and genotypes, identification of salmonella genomic island 1 (sgi1), detection of virulence genes, plasmid analysis, conjugal transfer experiments, and sequencing of class 1 integrons were conducted. multidrug-resistant salmonella detected were serovars st ... | 2010 | 20338699 |
| determination of water quality variables, endotoxin concentration, and enterobacteriaceae concentration and identification in southern high plains dairy lagoons. | the objectives of this study were to determine the concentration of endotoxin, determine 20 water quality variables, and identify and enumerate fungal and bacterial pathogens from united states southern high plains dairy lagoons and control lakes during summer and winter. water samples were collected in triplicate from the north, south, east, and west quadrants of each body of water. the mean (+/- sem) winter dairy lagoon endotoxin concentration was significantly higher (9,678+/-1,834 ng/ml) tha ... | 2010 | 20338428 |
| reactogenicity and immunogenicity of live attenuated salmonella enterica serovar paratyphi a enteric fever vaccine candidates. | eight salmonella enterica serovar paratyphi a strains were screened as candidates to create a live attenuated paratyphoid vaccine. based on biochemical and phenotypic criteria, four strains, rks2900, mgn9772, mgn9773 and mgn9779, were selected as progenitors for the construction of deltaphopq mutant derivatives. all strains were evaluated in vitro for auxotrophic phenotypes and sensitivity to deoxycholate and polymyxin b. all deltaphopq mutants were more sensitive to deoxycholate and polymyxin b ... | 2010 | 20338215 |
| detection and control of a nosocomial outbreak caused by salmonella newport at a large animal hospital. | nosocomial salmonellosis is often assumed to occur because infection control and surveillance practices are inadequate, but published evidence is lacking to support the related contention that rigorous application of these practices can impact the severity of outbreaks. | 2010 | 20337913 |
| the salmonella type iii secretion effector, salmonella leucine-rich repeat protein (slrp), targets the human chaperone erdj3. | effectors of the type iii secretion systems (t3ss) are key elements in the interaction between many gram-negative pathogens and their hosts. slrp is an effector that is translocated into the eukaryotic host cell through the two virulence-associated t3ss of salmonella enterica. we found previously that this effector is an e3 ubiquitin ligase for mammalian thioredoxin. here, we identified erdj3, an endoplasmic reticulum lumenal chaperone of the hsp40/dnaj family, as a new target for slrp. experime ... | 2010 | 20335166 |
| short n-terminal sequences package proteins into bacterial microcompartments. | hundreds of bacterial species produce proteinaceous microcompartments (mcps) that act as simple organelles by confining the enzymes of metabolic pathways that have toxic or volatile intermediates. a fundamental unanswered question about bacterial mcps is how enzymes are packaged within the protein shell that forms their outer surface. here, we report that a short n-terminal peptide is necessary and sufficient for packaging enzymes into the lumen of an mcp involved in b(12)-dependent 1,2-propaned ... | 2010 | 20308536 |
| fine-tuning synthesis of yersinia pestis lcrv from runaway-like replication balanced-lethal plasmid in a salmonella enterica serovar typhimurium vaccine induces protection against a lethal y. pestis challenge in mice. | a balanced-lethal plasmid expression system that switches from low-copy-number to runaway-like high-copy-number replication (pya4534) was constructed for the regulated delayed in vivo synthesis of heterologous antigens by vaccine strains. this is an antibiotic resistance-free maintenance system containing the asda gene (essential for peptidoglycan synthesis) as a selectable marker to complement the lethal chromosomal deltaasda allele in live recombinant attenuated salmonella vaccines (rasvs) suc ... | 2010 | 20308296 |
| effect of bovine lactoferrin in salmonella ser. typhimurium infection in mice. | lactoferrin (lf) has in vitro antimicrobial activity against gram-negative bacteria. salmonella enterica subsp. enterica serovar typhimurium causes systemic infection and acute diarrhea in humans, mainly in children younger than 2 years of age. the aim of the study was to determine the in vivo effect of bovine lf in salmonella ser. typhimurium infection in mice. 58 balb/c mice were employed. two hours before the infection with 300 microl of 10(7) cfu of salmonella ser. typhimurium, 29 mice recei ... | 2010 | 20306285 |
| thiol peroxidase protects salmonella enterica from hydrogen peroxide stress in vitro and facilitates intracellular growth. | at present, salmonella is considered to express two peroxiredoxin-type peroxidases, tsaa and ahpc. here we describe an additional peroxiredoxin, tpx, in salmonella enterica and show that a single tpx mutant is susceptible to exogenous hydrogen peroxide (h(2)o(2)), that it has a reduced capacity to degrade h(2)o(2) compared to the ahpcf and tsaa mutants, and that its growth is affected in activated macrophages. these results suggest that tpx contributes significantly to the sophisticated defense ... | 2010 | 20304995 |
| natural and synthetic compounds such as trimethoprim behave as inhibitors of efflux in gram-negative bacteria. | we hypothesized that small heterocyclic or nitrogen-containing compounds could act as rnd efflux pump inhibitors (epis). to ascertain possible epis, we sought to identify compounds that synergized with substrates of rnd efflux pumps for wild-type bacteria and those that overexpress an efflux pump, but had no synergistic activity against strains in which a gene encoding a component of the acrab-tolc efflux pump had been inactivated. | 2010 | 20304975 |
| characterisation of multidrug-resistant salmonella typhimurium 4,[5],12:i:- dt193 strains carrying a novel genomic island adjacent to the thrw trna locus. | in 2006, monophasic, multidrug-resistant salmonella enterica spp. enterica serovar 4,[5],12:i:- strains appeared as a novel serotype in germany, associated with large diffuse outbreaks and increased need for hospitalisation. the emerging 4,[5],12:i:- strains isolated from patients in germany belong mainly to phage type dt193 according to the anderson phage typing scheme for s. typhimurium (stm) and exhibit at least a tetra-drug resistance. the strains have been shown to harbour stm-specific gifs ... | 2010 | 20303825 |
| evaluation of suspension array analysis for detection of egg yolk antibodies against salmonella enteritidis. | salmonella enterica serovar enteritidis (se) is an important source of food-related diarrhoea in humans, and table eggs are considered the primordial source of contamination of the human food chain. using eggs collected at egg-packing stations as samples could be a convenient strategy to detect colonization of layer flocks. the aim of this study was to evaluate egg yolk anti-salmonella antibody detection using suspension array analysis. an egg yolk panel from contact-infected and non-colonized l ... | 2010 | 20303192 |
| expression of the avian-specific toll-like receptor 15 in chicken heterophils is mediated by gram-negative and gram-positive bacteria, but not tlr agonists. | toll-like receptors (tlrs) are a critical component of the innate immune response of mammalian and avian species. while most mammalian tlrs have been well characterized, the chicken-specific tlr15 has not been extensively studied. we recently demonstrated that tlr15 is differentially expressed between salmonella-susceptible-and-resistant chickens, indicating a potential role in the innate immune response to infection with salmonella. the aim of the present study was to gain better insight into t ... | 2010 | 20303182 |
| identification of the regulatory logic controlling salmonella pathoadaptation by the ssra-ssrb two-component system. | sequence data from the past decade has laid bare the significance of horizontal gene transfer in creating genetic diversity in the bacterial world. regulatory evolution, in which non-coding dna is mutated to create new regulatory nodes, also contributes to this diversity to allow niche adaptation and the evolution of pathogenesis. to survive in the host environment, salmonella enterica uses a type iii secretion system and effector proteins, which are activated by the ssra-ssrb two-component syst ... | 2010 | 20300643 |
| inhibition of the ptdins(5) kinase pikfyve disrupts intracellular replication of salmonella. | 3-phosphorylated phosphoinositides (3-ptdins) orchestrate endocytic trafficking pathways exploited by intracellular pathogens such as salmonella to gain entry into the cell. to infect the host, salmonellae subvert its normal macropinocytic activity, manipulating the process to generate an intracellular replicative niche. disruption of the ptdins(5) kinase, pikfyve, be it by interfering mutant, sirna-mediated knockdown or pharmacological means, inhibits the intracellular replication of salmonella ... | 2010 | 20300065 |
| the high-adhesive properties of the fimh adhesin of salmonella enterica serovar enteritidis are determined by a single f118s substitution. | the binding properties of low- and high-adhesive forms of fimh adhesins from salmonella enterica serovars enteritidis and typhimurium (s. enteritidis and s. typhimurium) were studied using chimeric proteins containing an additional peptide that represents an n-terminal extension of the fimf protein. this modification, by taking advantage of a donor strand exchange mechanism, closes the hydrophobic groove in the fimbrial domain of the fimh adhesin. such self-complemented adhesins (scfimh) did not ... | 2010 | 20299404 |
| listeria and salmonella bacterial vectors of tumor-associated antigens for cancer immunotherapy. | this review covers the use of the facultative intracellular bacteria, listeriamonocytogenes and salmonella enterica serovar typhimurium as delivery systems for tumor-associated antigens in tumor immunotherapy. because of their ability to infect and survive in antigen presenting cells, these bacteria have been harnessed to deliver tumor antigens to the immune system both as bacterially expressed proteins and encoded on eukaryotic plasmids. they do this in the context of strong innate immunity, wh ... | 2010 | 20299242 |
| sulphonamide investigations; treatment of experimental pasteurella septica, ery. rhusiopathiae and s. cholerae-suis infections in mice with sulphathiazole, sulphadiazine, sulphamezathine and sulphapyridine. | 1947 | 20265764 | |
| coronary arteritis with fatal thrombosis due to salmonella choleraesuis variety kunzendorf. | 1947 | 20262639 | |
| salicylate reduces the antimicrobial activity of ciprofloxacin against extracellular salmonella enterica serovar typhimurium, but not against salmonella in macrophages. | salicylate, a potent inducer of the mara activator in salmonella enterica, is the principal metabolite of aspirin, which is often consumed for medicinal and cosmetic uses. our research was aimed at testing if salicylate activates the mar regulon in macrophage-associated salmonella (intracellular bacteria), and investigating its effects on bacterial susceptibility to ciprofloxacin extracellularly and intracellularly. | 2010 | 20237076 |
| mechanisms and physiological effects of protamine resistance in salmonella enterica serovar typhimurium lt2. | protamines are cationic peptides that exert antimicrobial activity. we have examined the evolution of bacterial resistance to protamine sulphate and the resulting effects on fitness and physiology, with the objective of increasing knowledge about mechanisms of bacterial resistance to antimicrobial peptides. | 2010 | 20233778 |
| intrafamilial transmission of extended-spectrum-beta-lactamase-producing escherichia coli and salmonella enterica babelsberg among the families of internationally adopted children. | international adoption from developing countries has become an increasing phenomenon in recent years. given the high prevalence of multidrug-resistant (mdr) bacteria in these countries, the adopted children represent a group at risk for both carriage and infection with mdr bacteria. the dynamics of intrafamilial transmission of mdr bacteria after adoption was studied in a prospective study from january 2002 to january 2005. | 2010 | 20233775 |
| structural and functional characterization of three dsba paralogues from salmonella enterica serovar typhimurium. | in prototypic escherichia coli k-12 the introduction of disulfide bonds into folding proteins is mediated by the dsb family of enzymes, primarily through the actions of the highly oxidizing protein ecdsba. homologues of the dsb catalysts are found in most bacteria. interestingly, pathogens have developed distinct dsb machineries that play a pivotal role in the biogenesis of virulence factors, hence contributing to their pathogenicity. salmonella enterica serovar (sv.) typhimurium encodes an exte ... | 2010 | 20233716 |
| periplasmic peptidyl-prolyl isomerases sura and fkpa play an important role in the starvation-stress response (ssr) of salmonella enterica serovar typhimurium. | carbon-energy source (c)-starved cells of salmonella enterica serovar typhimurium (s. typhimurium) are remarkably more resistant to stress than actively growing ones. carbon-starved s. typhimurium is capable of withstanding extended periods of starvation and assault from a number of different stresses that rapidly kill growing cells. these unique properties of the c-starved cell are the direct result of a series of genetic and physiological adaptations referred to as the starvation-stress respon ... | 2010 | 20232248 |
| identification by pcr of non-typhoidal salmonella enterica serovars associated with invasive infections among febrile patients in mali. | in sub-saharan africa, non-typhoidal salmonella (nts) are emerging as a prominent cause of invasive disease (bacteremia and focal infections such as meningitis) in infants and young children. importantly, including data from mali, three serovars, salmonella enterica serovar typhimurium, salmonella enteritidis and salmonella dublin, account for the majority of non-typhoidal salmonella isolated from these patients. | 2010 | 20231882 |
| the multi-copper-ion oxidase cueo of salmonella enterica serovar typhimurium is required for systemic virulence. | salmonella enterica serovar typhimurium possesses a multi-copper-ion oxidase (multicopper oxidase), cueo (also known as cuid), a periplasmic enzyme known to be required for resistance to copper ions. cueo from s. typhimurium was expressed as a recombinant protein in escherichia coli, and the purified protein exhibited a high cuprous oxidase activity. we have characterized an s. typhimurium cueo mutant and confirmed that it is more sensitive to copper ions. using a murine model of infection, it w ... | 2010 | 20231415 |
| imaging type-iii secretion reveals dynamics and spatial segregation of salmonella effectors. | the type-iii secretion system (t3ss) enables gram-negative bacteria to inject effector proteins into eukaryotic host cells. upon entry, t3ss effectors work cooperatively to reprogram host cells, enabling bacterial survival. progress in understanding when and where effectors localize in host cells has been hindered by a dearth of tools to study these proteins in the native cellular environment. we report a method to label and track t3ss effectors during infection using a split-gfp system. we demo ... | 2010 | 20228815 |
| accuracy and sensitivity of commercial pcr-based methods for detection of salmonella enterica in feed. | the present study compared the performance of commercial pcr-based salmonella enterica detection methods (bax system q7, the iq-check salmonella ii kit, and the taqman salmonella enterica detection kit) with culture-based methods (modified semisolid rappaport-vassiliadis [msrv] and nmkl71) in spiked and naturally contaminated samples of feed mill scrapings (fms), palm kernel meal (pkm), pelleted feed (pf), rape seed meal (rsm), soybean meal (sm), and wheat grain (wg). when results from the vario ... | 2010 | 20228106 |
| cryptdin-2: a novel therapeutic agent for experimental salmonella typhimurium infection. | salmonella infections represent a major health hazard and have been responsible for a number of epidemics. in view of the emergence of multidrug-resistant salmonella strains, there is a need for therapeutic alternatives. the purpose of this study was to evaluate the therapeutic potential of cryptdin-2 (a paneth cell antimicrobial peptide) against salmonella infection. | 2010 | 20228082 |
| kinetics of growth and inactivation of salmonella enterica serotype typhimurium dt104 in pasteurised liquid egg products. | the potential impact of post-pasteurisation contamination of liquid egg products with the multi-antibiotic resistant pathogen salmonella enterica serotype typhimurium definitive type 104 (dt104) was assessed by determining the viability of this bacterium in whole egg, albumen and 10% w/w sugared and salted yolk incubated at 4-42 degrees c. results indicated that populations of s. typhimurium dt104 were slowly inactivated in all four products when stored at 4 degrees c. however, based on the typi ... | 2010 | 20227605 |
| the salmonella enterica serovar typhimurium qseb response regulator negatively regulates bacterial motility and swine colonization in the absence of the qsec sensor kinase. | salmonella enterica serovar typhimurium (s. typhimurium) responds to the catecholamine, norepinephrine by increasing bacterial growth and enhancing motility. in this study, iron with or without the siderophore, ferrioxamine e also enhanced bacterial motility. iron-enhanced motility was growth-rate dependent, while norepinephrine-enhanced motility was growth-rate independent. the outer membrane catecholate receptors, iron, fepa and cira (required for norepinephrine-enhanced growth) were not requi ... | 2010 | 20227482 |
| in vitro antagonistic activities of animal intestinal strains against swine-associated pathogens. | a wide range of enteropathogens cause costly diarrhoeal diseases in fattening piglets and account for food-related infections in humans. the objective of this study was to screen beneficial bacterial strains from the gastrointestinal tract of various animal sources for antagonistic activity against diverse pathogens associated with hazardous pig production times. using agar spot assays, 15 well-characterized strains belonging to lactobacillus, enterococcus, bifidobacterium and bacillus were stud ... | 2010 | 20226602 |
| inactivation of salmonella enteritidis strains by combination of high hydrostatic pressure and nisin. | the effects of high hydrostatic pressure (hhp) and nisin treatment alone and in combination on cellular components and viability of two salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis) strains were evaluated by differential scanning calorimetry (dsc) and plate counting in order to evaluate the relative resistance and optimize the treatment conditions. s. enteritidis fda and osu 799 strains were subjected to hhp (0.1-550 mpa for 10 min at 25 degrees c) alone and in combina ... | 2010 | 20226554 |
| differences in attachment of salmonella enterica serovars to cabbage and lettuce leaves. | this study investigated the ability of five salmonella enterica serovars to attach to and colonize intact and cut lettuce (iceberg, romaine) and cabbage surfaces. biofilm formation and attachment of salmonella serovars to intact and cut leaves were determined. populations of loosely and strongly attached salmonella were obtained to calculate the attachment strength (s(r)). biofilm formation, as determined by microtiter plate assay, varied with strain and growth medium used. salmonella tennessee ... | 2010 | 20226552 |
| influence of 5 major salmonella pathogenicity islands on nk cell depletion in mice infected with salmonella enterica serovar enteritidis. | in this study we were interested in the colonisation and early immune response of balb/c mice to infection with salmonella enteritidis and isogenic pathogenicity island free mutants. | 2010 | 20226037 |
| ppgpp-mediated stationary phase induction of the genes encoded by horizontally acquired pathogenicity islands and cob/pdu locus in salmonella enterica serovar typhimurium. | salmonella enterica is highly diverse in terms of genome structure, which is at least partly due to the horizontal transfer of genetic elements from various sources. in this study, we examined the expression profiles of such genes in salmonella pathogenicity islands (spis) and the cob/pdu locus, horizontally acquired large dna segments, during growth under standard growth conditions. transcripts from exponentially growing and early stationary phase salmonellae were compared using various methods ... | 2010 | 20221735 |
| differential inhibition of human immunodeficiency virus type 1 in peripheral blood mononuclear cells and tzm-bl cells by endotoxin-mediated chemokine and gamma interferon production. | bacterial lipopolysaccharide (endotoxin) is a frequent contaminant of biological specimens and is also known to be a potent inducer of beta-chemokines and other soluble factors that inhibit hiv-1 infection in vitro. though lipopolysaccharide (lps) has been shown to stimulate the production of soluble hiv-1 inhibitors in cultures of monocyte-derived macrophages, the ability of lps to induce similar inhibitors in other cell types is poorly characterized. here we show that lps exhibits potent anti- ... | 2010 | 20218881 |
| the tandem inversion duplication in salmonella enterica: selection drives unstable precursors to final mutation types. | during growth under selection, mutant types appear that are rare in unselected populations. stress-induced mechanisms may cause these structures or selection may favor a series of standard events that modify common preexisting structures. one such mutation is the short junction (sj) duplication with long repeats separated by short sequence elements: ab*(cd)*(cd)*e (* = a few bases). another mutation type, described here, is the tandem inversion duplication (tid), where two copies of a parent seq ... | 2010 | 20215473 |
| the c terminus of sipc binds and bundles f-actin to promote salmonella invasion. | salmonella enterica serovar typhimurium invade non-phagocytic cells by injecting bacterial effector proteins to exploit the host actin cytoskeleton network. sipc is such a salmonella effector known to nucleate actin, bundle f-actin, and translocate type iii effectors. the molecular mechanism of how sipc bundles f-actin and sipc domains responsible for these activities are not well characterized. we successfully separated these activities through a series of genetic deletion/insertions in sipc. w ... | 2010 | 20212042 |
| synergistic antimicrobial effect of nisin and p-cymene on salmonella enterica serovar typhi in vitro and on ready-to-eat food. | foods contaminated with salmonella enterica serovar typhi are a major cause of typhoid fever, leading to public health problems and economic losses worldwide. nisin and rho-cymene were tested in this study for their antimicrobial activity against s. typhi at 4 degrees c and 37 degrees c. nisin and rho-cymene, when used separately, did not inhibit the bacterium at either temperature. a synergistic antimicrobial effect between both compounds was observed when they were used simultaneously. this sy ... | 2010 | 20208367 |
| salmonella isolated from the feces of migrating cranes at the izumi plain (2002-2008): serotype, antibiotic sensitivity and pfge type. | from november 2002 to february 2008, 2,251 crane feces were collected at the izumi plain in kagoshima prefecture. salmonella enterica was isolated from 359 feces (15.9%), of which 332 (92.5%) were salmonella typhimurium (st), 9 were s. hvittingfoss/ii, 4 were s. abaetetuba, 3 were s. enteritidis, 2 were s. konstanz, 1 was s. pakistan and 8 were untyped isolates, respectively. against 12 antimicrobial agents, no resistant strains were found in 154 isolates examined, but one was found to be resist ... | 2010 | 20203434 |
| synergistic effects of ethanol and uv radiation to reduce levels of selected foodborne pathogenic bacteria. | the purpose of this study was to determine whether combined treatments would produce synergistic disinfection effects on food products during food processing compared with single treatments. we investigated the bactericidal effects of a commercial chemical disinfectant (ethanol) and of uv radiation on bacillus cereus f4810/72, cronobacter sakazakii kctc 2949, staphylococcus aureus atcc 35556, escherichia coli atcc 10536, and salmonella enterica typhimurium no/na in vitro. various concentrations ... | 2010 | 20202345 |
| lessons learned from the management of a national outbreak of salmonella ohio linked to pork meat processing and distribution. | during the summer of 2005, an increase in reports of human cases of salmonella enterica serovar ohio infection was observed in belgium. during 11 weeks, between 1 july and 13 september, 60 cases of laboratory-confirmed salmonella ohio infection were reported to the national reference centre for salmonella, with a peak onset of symptoms in the third week of july. all clinical isolates caused self-limiting gastroenteritis; both genders (32 males and 28 females) and all age groups (three children < ... | 2010 | 20202340 |
| probiotics down-regulate genes in salmonella enterica serovar typhimurium pathogenicity islands 1 and 2. | salmonella typhimurium pathogenesis relies mainly on the expression of genes of two pathogenicity islands, salmonella pathogenicity islands 1 and 2 (spi1 and spi2). each island has its own pattern of expression and regulation. success in suppression of the responsible key activator of each island would be an effective way of controlling salmonella, especially with the emerging problem of antibiotic-resistant strains. probiotics have been shown to inhibit several foodborne pathogens, and their mo ... | 2010 | 20202329 |
| salmonella pathogenicity island 1 differentially modulates bacterial entry to dendritic and non-phagocytic cells. | salmonella enterica serovar typhimurium can enter non-phagocytic cells, such as intestinal epithelial cells, by virtue of a type three secretion system (ttss) encoded in the salmonella pathogenicity island 1 (spi-1), which translocates bacterial effector molecules into the host cell. salmonella can also be taken up by dendritic cells (dcs). although the role of spi-1 in non-phagocytic cell invasion is well established, its contribution to invasion of phagocytic cells has not been evaluated. here ... | 2010 | 20201987 |
| structure of the o-polysaccharide of salmonella enterica o41. | an o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of salmonella enterica o41, and the following structure of the o-unit was determined by chemical analyses along with 1d and 2d (1)h and (13)c nmr spectroscopy: -->2)-beta-d-manp-(1-->4)-alpha-d-glcp-(1-->3)-alpha-l-quipnac-(1-->3)-alpha-d-glcpnac-(1--> where quinac stands for 2-acetamido-2,6-dideoxyglucose. the structure established is in agreement with the o-antigen gene cluster of s. enterica o41 and tentative ... | 2010 | 20199769 |
| chronic murine typhoid fever is a natural model of secondary hemophagocytic lymphohistiocytosis. | hemophagocytic lymphohistiocytosis (hlh) is a hyper-inflammatory clinical syndrome associated with neoplastic disorders especially lymphoma, autoimmune conditions, and infectious agents including bacteria, viruses, protozoa and fungi. in both human and veterinary medicine, hemophagocytic histiocytic disorders are clinically important and frequently fatal. hlh in humans can be a primary (familial, autosomal recessive) or secondary (acquired) condition, with both types generally precipitated by an ... | 2010 | 20195482 |
| salmonella enterica serovar typhimurium-induced placental inflammation and not bacterial burden correlates with pathology and fatal maternal disease. | food-borne infections caused by salmonella enterica species are increasing globally, and pregnancy poses a high risk. pregnant mice rapidly succumb to s. enterica serovar typhimurium infection. to determine the mechanisms involved, we addressed the role of inflammation and bacterial burden in causing placental and systemic disease. in vitro, choriocarcinoma cells were a highly conducive niche for intracellular s. typhimurium proliferation. while infection of mice with s. typhimurium wild-type (w ... | 2010 | 20194592 |
| anti-infective mechanisms induced by a probiotic lactobacillus strain against salmonella enterica serovar typhimurium infection. | the prevention of pathogen infections is one of the most extensively studied effects of probiotics. l. casei crl 431 is a probiotic bacterium and its effects on the gut immune cells have been extensively studied. the aim of the present study was to determine, using a mouse model, the preventive and therapeutic effect of l. casei crl 431 to achieve protection against salmonella enteritidis serovar typhimurium infection. in both previous and continuous (previous and post-infection) probiotic admin ... | 2010 | 20193971 |
| [study on the epidemiological characteristics and molecular typing of salmonella enterica subsp. enterica serovar senftenberg in shanghai]. | to study the molecular epidemiological characteristics of salmonella enterica subsp. enterica serovar senftenberg (salmonella senftenberg) in shanghai, from 2006 to 2007. | 2009 | 20193231 |
| drug resistance patterns in salmonella enterica subspecies enterica serotype typhi strains isolated over a period of two decades, with special reference to ciprofloxacin and ceftriaxone. | fluoroquinolone-resistant salmonella enterica subspecies enterica serotype typhi are being increasingly reported from the asian subcontinent. this has been hypothesised to be due to a double mutation in the gyra gene. a total of 113 s. typhi strains isolated during 1987-2006 in a tertiary-level hospital of north india were monitored for their antibiotic susceptibility by the disk diffusion and minimum inhibitory concentration (mic) methods. the study period was arbitrarily divided into four equa ... | 2010 | 20188522 |
| sub-iliac lymph nodes at slaughter lack ability to predict salmonella enterica prevalence for swine farms. | the aim of this study was to assess the value of deep systemic sub-iliac lymph nodes collected at slaughter as predictors of salmonella prevalence in live hogs. an observational study was conducted on 24 farms from september 2006 to february 2009. at least one cohort of market-weight pigs was visited for each farm. within each cohort, 30 farm fecal samples on farm and 30 sub-iliac lymph nodes from matched pigs at slaughter were collected. samples were cultured for salmonella enterica and serotyp ... | 2010 | 20187754 |
| salmonella enterica serotype cerro among dairy cattle in new york: an emerging pathogen? | the focus of this study was salmonella enterica serotype cerro, a potentially emerging pathogen of cattle. our objectives were to document the within-herd prevalence of salmonella cerro among a sample of new york dairy herds, to describe the antimicrobial resistance patterns and pulsed-field gel electrophoresis types of the isolates, and to elucidate the status of this serotype as a bovine pathogen. data were collected prospectively from dairy herds throughout new york that had at least 150 lact ... | 2010 | 20187753 |
| salmonella pathogenicity island 1 (spi-1) type iii secretion of sopd involves n- and c-terminal signals and direct binding to the invc atpase. | salmonella enterica serovar typhimurium (s. typhimurium) is an important pathogen and a causative agent of gastroenteritis. during infection, s. typhimurium assembles molecular-needle complexes termed type iii secretion (t3s) systems to translocate effector proteins from the bacterial cytoplasm directly into the host cell. the t3s signals that direct the secretion of effectors still remain enigmatic. sopd is a key t3s effector contributing to the systemic virulence of s. typhimurium and the deve ... | 2010 | 20185511 |
| relatedness of the o-polysaccharide structures of escherichia coli o123 and salmonella enterica o58, both containing 4,6-dideoxy-4-{n-[(s)-3-hydroxybutanoyl]-d-alanyl}amino-d-glucose; revision of the e. coli o123 o-polysaccharide structure. | o-polysaccharides were isolated by mild acid degradation of the lipopolysaccharides of escherichia coli o123 and salmonella enterica o58 and studied by chemical methods and 2d (1)h and (13)c nmr spectroscopy, including experiments in a h(2)o/d(2)o mixture, which enabled observation of correlations for nitrogen-linked protons. the following structure of the o-polysaccharide of e. coli o123 was established: -->3)-beta-d-quip4nalahb-(1-->6)-alpha-d-glcpnac-(1-->3)-alpha-l-quipnac-(1-->3)-alpha-d-gl ... | 2010 | 20181329 |
| sialic acid is required for nonspecific adherence of salmonella enterica ssp. enterica serovar typhi on caco-2 cells. | to initiate infection, bacteria must adhere to and colonize host tissues. specific and nonspecific mechanisms participate in the adherence process. salmonella enterica ssp. enterica serovar typhi (s. typhi) must first adhere to the intestinal epithelium to invade and disseminate throughout the host. in this study, the role of colonic epithelial cell surface sialic acid in the adherence of s. typhi was defined. neuraminidase treatment of colonic caco-2 cells removed 27-58% of surface sialic acid. ... | 2010 | 20180850 |
| pulsed-field gel electrophoresis diversity of human and bovine clinical salmonella isolates. | pulsed-field gel electrophoresis (pfge) characterization of 335 temporally and spatially matched clinical, bovine, and human salmonella enterica subsp. enterica isolates revealed 167 xbai pfge patterns. these isolates were previously classified into 51 serotypes and 73 sequence types, as determined by multilocus sequence typing. discriminatory power of pfge (simpson's index, d = 0.991) was considerably higher than that of multilocus sequence typing (d = 0.920) or serotyping (d = 0.913). although ... | 2010 | 20180633 |
| proton-conductivity assay of plugged and unplugged mota/b proton channel by cytoplasmic phluorin expressed in salmonella. | mota and motb form the proton-channel complex of the proton-driven bacterial flagellar motor. a plug segment of escherichia coli motb suppresses proton leakage through the mota/b complex when it is not assembled into the motor. using a ratiometric ph indicator protein, phluorin, we show that the proton-conductivity of a salmonella mota/b complex not incorporated into the motor is two orders of magnitude lower than that of a complex that is incorporated and activated. this leakage is, however, si ... | 2010 | 20178785 |
| amplification of the gene for isoleucyl-trna synthetase facilitates adaptation to the fitness cost of mupirocin resistance in salmonella enterica. | mutations that cause resistance to antibiotics in bacteria often reduce growth rate by impairing some essential cellular function. this growth impairment is expected to counterselect resistant organisms from natural populations following discontinuation of antibiotic therapy. unfortunately (for disease control) bacteria adapt and improve their growth rate, often without losing antibiotic resistance. this adaptation process was studied in mupirocin-resistant (mup(r)) strains of salmonella enteric ... | 2010 | 20176977 |
| gallstones play a significant role in salmonella spp. gallbladder colonization and carriage. | salmonella enterica serovar typhi can colonize the gallbladder and persist in an asymptomatic carrier state that is frequently associated with the presence of gallstones. we have shown that salmonellae form bile-mediated biofilms on human gallstones and cholesterol-coated surfaces in vitro. here, we test the hypothesis that biofilms on cholesterol gallbladder stones facilitate typhoid carriage in mice and men. naturally resistant (nramp1(+/+)) mice fed a lithogenic diet developed cholesterol gal ... | 2010 | 20176950 |
| an antimicrobial peptide that targets dna repair intermediates in vitro inhibits salmonella growth within murine macrophages. | the hexapeptide wrwycr was previously identified on the basis of its ability to inhibit bacteriophage lambda integrase-mediated recombination by trapping and preventing resolution of the holliday junction intermediate. this peptide inhibits several unrelated dna repair enzymes that bind to and process holliday junctions and branched dna substrates. wrwycr and its d stereoisomer, wrwycr, are bactericidal against both gram-positive and gram-negative bacteria, causing the accumulation of dna breaks ... | 2010 | 20176906 |
| autonomous growth of isolated single listeria monocytogenes and salmonella enterica serovar typhimurium cells in the absence of growth factors and intercellular contact. | the aim of this study was to observe growth of isolated single bacterial cells in the absence of growth factors and intercellular contact. in order to exclude stochastic uncertainties induced by dilution series, a new micromanipulation method was developed to ensure explicit results under visual control. this was performed with particular care for production of single prokaryotic cells and subsequent investigation of their autonomous growth. over 450 single isolated listeria monocytogenes and sa ... | 2010 | 20173058 |
| spontaneous excision of the salmonella enterica serovar enteritidis-specific defective prophage-like element phise14. | salmonella enterica serovar enteritidis has emerged as a major health problem worldwide in the last few decades. dna loci unique to s. enteritidis can provide markers for detection of this pathogen and may reveal pathogenic mechanisms restricted to this serovar. an in silico comparison of 16 salmonella genomic sequences revealed the presence of an approximately 12.5-kb genomic island (gei) specific to the sequenced s. enteritidis strain nctc13349. the gei is inserted at the 5' end of gene ydao ( ... | 2010 | 20172996 |
| immunogenic properties of a recombinant fusion protein containing the c-terminal 19 kda of plasmodium falciparum merozoite surface protein-1 and the innate immunity agonist flic flagellin of salmonella typhimurium. | in a recent study, we demonstrated the immunogenic properties of a new malaria vaccine polypeptide based on a 19 kda c-terminal fragment of the merozoite surface protein-1 (msp1(19)) from plasmodium vivax and an innate immunity agonist, the salmonella enterica serovar typhimurium flagellin (flic). herein, we tested whether the same strategy, based on the msp1(19) component of the deadly malaria parasite plasmodium falciparum, could also generate a fusion polypeptide with enhanced immunogenicity. ... | 2010 | 20170765 |
| [a 14-year-old healthy boy with splenic abscess due to salmonella enterica serovar senftenberg]. | salmonella enterica serovar senftenberg may very rarely cause splenic abscess, which can be diagnosed using gallium scintigraphy and drained. a 14-year-old boy admitted for stomachache, diarrhea and fever and diagnosed from his symptoms as having enteritis did not respond when treated with fosfomycin, meropenem, and clindamycin. a low-density splenic area seen in abdominal computed tomography on admission did not show contrast medium enhancement. gallium scintigraphy on hospital day 10, however, ... | 2010 | 20170018 |
| [antimicrobial susceptibility and resistance genes among salmonella enterica serovar weltevreden isolates from humans, farm animals, and the environment in okinawa prefecture between 1992 and 2007]. | between 1992 and 2007, a total of 86 isolates of salmonella enterica weltevreden were obtained from clinical human samples (n = 41), 45 farm animals and their environment on 20 farms, including poultry (n = 25), beef cattle (n = 5), swine (n = 5), dairy cattle (n = 3), mice (n = 2), pony (n = 1), fly (n = 1) and feed samples (n = 3), in okinawa prefecture, japan. only seven isolates (8.1%) of the isolates were resistance to one or more antimicrobial agents tested; six streptomycin (7.0%), six ox ... | 2010 | 20170010 |
| cell biology. rise of the rival. | 2010 | 20167774 | |
| attenuated salmonella choleraesuis-mediated rnai targeted to conserved regions against foot-and-mouth disease virus in guinea pigs and swine. | in this study, specific sequences within three genes (3d, vp4 and 2b) of the foot-and-mouth disease virus (fmdv) genome were determined to be effective rnai targets. these sequences are highly conserved among different serotype viruses based on sequence analysis. small interfering rna (sirna)-expressing plasmids (p3d-nt19, p3d-nt56, pvp4-nt19, pvp4-nt65 and p2b-nt25) were constructed to express sirna targeting 3d, vp4 and 2b, respectively. the antiviral potential of these sirna for various fmdv ... | 2010 | 20167192 |
| chromagar salmonella detection test kit. performance tested method 020502. | bbl chromagar salmonella was evaluated by an external food testing laboratory for the recovery of salmonella in peanut butter using the u.s. food and drug administration's bacteriological analytical manual (fda-bam) procedure. the peanut butter was found to be negative for the presence of salmonella and, therefore, was seeded with heat-stressed salmonella at target concentrations of 0.2 and 2 cfu/25 g. the salmonella-seeded samples remained at room temperature for 14 days before analysis to stab ... | 2009 | 20166616 |
| dupont qualicon bax system polymerase chain reaction assay. performance tested method 100201. | a recent outbreak of salmonella in peanut butter has highlighted the need for validation of rapid detection methods. a multilaboratory study for detecting salmonella in peanut butter was conducted as part of the aoac research institute emergency response validation program for methods that detect outbreak threats to food safety. three sites tested spiked samples from the same master mix according to the u.s. food and drug administration's bacteriological analytical manual (fda-bam) method and th ... | 2009 | 20166615 |
| taqman salmonella enterica detection kit. performance tested method 020803. | peanut butter spiked with salmonella enterica ser. typhimurium was prepared by an independent laboratory and sent to applied biosystems to determine the sensitivity and specificity of the taqman salmonella enterica detection kit for detecting salmonella in peanut butter. the samples were spiked at three levels: five no-spike (0 cfu/25 g); 20 low-spike (0.2 cfu/25 g); and 20 high-spike (2 cfu/25 g). they were coded to create a blind set of 45 samples. the samples were processed based on an unpair ... | 2009 | 20166614 |
| singlepath salmonella. performance tested method 060401. | singlepath salmonella is an immunochromatographic (lateral flow) assay for the presumptive qualitative detection of salmonella spp. in food. a previous aoac performance tested method study evaluated singlepath salmonella as an effective method for the detection of salmonella spp. in the following selected foods: dried skimmed milk, black pepper, dried pet food, desiccated coconut, cooked peeled frozen prawns, raw ground beef, and raw ground turkey. in this emergency response validation extension ... | 2009 | 20166612 |
| foodproof salmonella detection kit. performance tested method 120301. | the foodproof salmonella detection kit was previously validated in the performance tested methods program for the detection of salmonella species in a variety of foods, including milk powder, egg powder, coconut, cocoa powder, chicken breast, minced meat, sliced sausage, sausage, smoked fish, pasta, white pepper, cumin, dough, wet pet food, dry pet food, ice cream, watermelon, sliced cabbage, food dye, and milk chocolate. the method was shown to be equivalent to the u.s. food and drug administra ... | 2009 | 20166611 |
| rapid'salmonella chromogenic medium. performance tested method 050701. | rapid'salmonella is a chromogenic medium for isolation and detection of salmonella spp. in food, based on two enzymatic activities. all presumptive salmonella-positive colonies are magenta, including lactose-positive salmonella. s. typhi, and s. paratyphi serotypes, due to detection of c8 esterase activity. in order to differentiate salmonella from other enterobacteriaceae, the medium includes a second chromogenic substrate. as part of an emergency response validation due to a massive outbreak a ... | 2009 | 20166610 |
| iq-check salmonella ii: real-time polymerase chain reaction test kit. performance tested method 010803. | iq-check salmonella ii is a real-time pcr kit for detection of salmonella in foods. specific oligonucleotide probes are used to detect target dna during the amplification, by hybridizing to the amplicons. these probes are linked to a fluorophore, which fluoresces only when hybridized to the target sequence. as part of an emergency response validation due to a massive outbreak and subsequent recall, peanut butter was tested to compare the performance of iq-check salmonella ii to the u.s. food and ... | 2009 | 20166609 |
| transia plate salmonella gold. performance tested method 010602. | a study was conducted to determine the efficacy of detecting low contamination levels of salmonella in peanut butter using transia plate salmonella gold (tpsg). this study was conducted under the aoac emergency response validation program. a set of samples was each analyzed by the tpsg method and the u.s. food and drug administration's bacteriological analytical manual reference method. among the 45 total samples and controls, 26 were positive by the tpsg and 24 were positive by the reference me ... | 2009 | 20166607 |
| 1-2 test. | a study was conducted to determine the efficacy of detecting low contamination levels of salmonella in peanut butter using the 1-2 test. this study was conducted under the aoac research institute emergency response validation program. a set of samples was analyzed by the 1-2 test and the u.s. food and drug administration's bacteriological analytical manual reference method. among the 90 total samples and controls, 32 samples were positive by the 1-2 test and the reference method. statistical ana ... | 2009 | 20166605 |
| development of a multiplex primer extension assay for rapid detection of salmonella isolates of diverse serotypes. | food-borne salmonellosis is a major manifestation of gastrointestinal disease in humans across the globe. accurate and rapid identification methods could positively impact the identification of isolates, enhance outbreak investigation, and aid infection control. the snapshot multiplex system is a primer extension-based method that enables multiplexing of single nucleotide polymorphisms (snps). here the method has been developed for the identification of five salmonella serotypes, commonly detect ... | 2010 | 20164272 |
| evaluation of pet-related management factors and the risk of salmonella spp. carriage in pet dogs from volunteer households in ontario (2005-2006). | the purpose of this study was to determine pet-related management factors that may be associated with the presence of salmonella spp. in feces of pet dogs from volunteer households. from october 2005 until may 2006, 138 dogs from 84 households in ontario were recruited to participate in a cross-sectional study. five consecutive daily fecal samples were collected from each dog and enrichment culture for salmonella spp. was performed. a higher than expected number of the dogs (23.2%; 32/138) had a ... | 2011 | 20163574 |
| comparative study of leaf and stem bark extracts of parkia biglobosa against enterobacteria. | hydroethanolic and aqueous extracts of leaf and stem bark of parkia biglobosa (jacq) benth. (mimosaceae) were tested against clinical isolates escherichia coli, salmonella typhi, shigella dysenteriae and enterococcus faecalis, and corresponding collection strains e. coli cip 105 182, salmonella enterica cip 105 150, shigella dysenteriae cip 54-51 and enterococcus faecalis cip 103 907. discs of gentamicin, a broad spectrum antibiotic were used as positive controls. the results showed that all the ... | 2008 | 20161943 |
| virulence mechanisms displayed by salmonella to impair dendritic cell function. | dendritic cells (dcs) link innate and adaptive immunity by directly recognizing pathogen-associated molecular patterns (pamps) on bacteria. dcs can capture and degrade bacteria and present their antigens on mhc molecules to t cells. pamp recognition promotes dc maturation, a phenotypic change that empowers them to prime naïve t cells. as a result, an adaptive immune response that specifically targets bacteria-derived antigens is initiated. consequently, any impairment of dc function might contri ... | 2010 | 20158475 |
| eric pcr and rapd based fingerprinting of salmonella typhi strains isolated over a period of two decades. | salmonella enterica serotype typhi strains (n=113) were isolated from typhoid patients over a period of 2 decades, i.e. 1987-2006. rapd and eric pcr methods were used for random whole genome typing of these strains. eric pcr was found to be very efficient with the discriminatory index (di) of 0.9821 with 100% reproducibility. rapd was satisfactory in discriminating the strains (di=0.8978) but with poor reproducibility (40%). however, composite genotypic analysis was still better with di of 0.998 ... | 2010 | 20156601 |
| the occurrence and sources of campylobacter spp., salmonella enterica and escherichia coli o157:h7 in the salmon river, british columbia, canada. | in this study, we wished to assess the prevalence and determine the sources of three zoonotic bacterial pathogens (salmonella, campylobacter, and escherichia coli o157:h7) in the salmon river watershed in southwestern british columbia. surface water, sewage, and animal faecal samples were collected from the watershed. selective bacterial culture and pcr techniques were used to isolate these three pathogens and indicator bacteria from these samples and characterize them. campylobacter was the mos ... | 2010 | 20154400 |
| the second extracellular loop of pore-forming subunits of atp-binding cassette transporters for basic amino acids plays a crucial role in interaction with the cognate solute binding protein(s). | in the thermophile geobacillus stearothermophilus, the uptake of basic amino acids is mediated by an abc transporter composed of the substrate binding protein (receptor) artj and a homodimer each of the pore-forming subunit, artm, and the nucleotide-binding subunit, artp. we recently identified two putative binding sites in artj that might interact with the art(mp)(2) complex, thereby initiating the transport cycle (a. vahedi-faridi et al., j. mol. biol. 375:448-459, 2008). here we investigated ... | 2010 | 20154136 |
| protecting against antimicrobial effectors in the phagosome allows sodcii to contribute to virulence in salmonella enterica serovar typhimurium. | salmonella enterica serovar typhimurium replicates in macrophages, where it is subjected to antimicrobial substances, including superoxide, antimicrobial peptides, and proteases. the bacterium produces two periplasmic superoxide dismutases, sodci and sodcii. although both are expressed during infection, only sodci contributes to virulence in the mouse by combating phagocytic superoxide. the differential contribution to virulence is at least partially due to inherent differences in the sodci and ... | 2010 | 20154132 |