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salmonella-allies in the fight against cancer.cancer has become the second ranking cause of death in the industrialized world. conventional anti-cancer therapies such as surgery, radiotherapy, and chemotherapy are effective in the treatment of solid tumors only to some extent. furthermore, they are often associated with severe side effects. use of bacteria as alternative cancer therapeutics has sporadically been followed over more than a century. the potential to target and colonize solid tumors could be shown for many different bacteria in ...201020526574
use of a mixture of bacteriophages for biological control of salmonella enterica strains in compost.bacteriophages specific to salmonella strains were isolated from sewage effluent and characterized. a five-strain bacteriophage mixture was applied to dairy manure compost inoculated with salmonella enterica serotype typhimurium. bacteriophage treatment resulted in a greater than 2-log-unit reduction of salmonella within 4 h at all moisture levels compared to the controls.201020525859
plasmid fingerprinting and virulence gene detection among indigenous strains of salmonella enterica serovar enteritidis.salmonella enterica serovar enteritidis is an important frequently reported zoonotic pathogen and a common cause of human gastroenteritis worldwide. the highly conserved sero-specific plasmids (ssps) and salmonella plasmid virulence (spy) genes have been shown to mediate extra-intestinal colonization and systemic infection. the objective of current study was to document the presence of ssps and spvb/spvc genes prevailing in the indigenous population of serovar enteritidis.200920524477
splice-variant 1 of the ancient domain protein 2 (acdp2) complements the magnesium-deficient growth phenotype of salmonella enterica sv. typhimurium strain mm281.evidence arguing for the existence of genes encoding for proteins directly involved in the transport of mg2+ through the cytoplasmic membrane have accumulated over the last few years. gene acdp2 (ancient conserved domain protein 2; old name cnnm2, cyclin m2) is one such gene. acdp2 is a distant homologue of the bacterial gene corc, which is known to be involved in cobalt resistance. we have previously demonstrated that the over-expression of the human mg2+ carrier slc41a1 partly complements the ...201020519162
salmonella enterica replication in hemophagocytic macrophages requires two type three secretion systems.salmonella enterica serotype typhimurium is a natural pathogen of mice, which acquire the bacteria orally and develop systemic acute infections that can become subacute to chronic infections. s. typhimurium can reside within hemophagocytic macrophages (hms) in sv129s6 mice, an slc11a1/nramp1(+/+) inbred strain. hms are activated macrophages which have ingested viable hematopoietic cells and are a key characteristic of infectious and inflammatory diseases. here we show that modest s. typhimurium ...201020515933
absence of poly(adp-ribose) polymerase 1 delays the onset of salmonella enterica serovar typhimurium-induced gut inflammation.the immune system comprises an innate and an adaptive immune response to combat pathogenic agents. the human enteropathogen salmonella enterica serovar typhimurium invades the intestinal mucosa and triggers an early innate proinflammatory host gene response, which results in diarrheal disease. several host factors, including transcription factors and transcription coregulators, are involved in the acute early response to salmonella infection. we found in a mouse model of enterocolitis induced by ...201020515923
elucidation of antimicrobial susceptibility profiles and genotyping of salmonella enterica isolates from clinical cases of salmonellosis in new mexico in 2008.in this study, we investigated the antimicrobial susceptibility profiles and the distribution of some well known genetic determinants of virulence in clinical isolates of salmonella enterica from new mexico. the minimum inhibitory concentrations (mics) for various antimicrobials were determined by using the e-test strip method according to clsi guidelines. virulence genotyping was performed by polymerase chain reaction (pcr) using primers specific for known virulence genes of salmonella enterica ...201020514366
a 96-well plate fluorescence assay for assessment of cellular permeability and active efflux in salmonella enterica serovar typhimurium and escherichia coli.multiply antibiotic-resistant (mar) mutants of escherichia coli and salmonella enterica are characterized by reduced susceptibility to several unrelated antibiotics, biocides and other xenobiotics. porin loss and/or active efflux have been identified as a key mechanisms of mar. a single rapid test was developed for mar.201020513705
mutations affecting export and activity of cytolysin a from escherichia coli.cytolysin a (known as clya, hlye, and shea) is a cytolytic pore-forming protein toxin found in several escherichia coli and salmonella enterica strains. the structure of its water-soluble monomeric form and that of dodecameric clya pores is known, but the mechanisms of clya export from bacterial cells and of pore assembly are only partially understood. here we used site-directed mutagenesis to study the importance of different regions of the e. coli clya protein for export and activity. the data ...201020511497
a multiplex single nucleotide polymorphism typing assay for detecting mutations that result in decreased fluoroquinolone susceptibility in salmonella enterica serovars typhi and paratyphi a.decreased susceptibility to fluoroquinolones has become a major problem for the successful therapy of human infections caused by salmonella enterica, especially the life-threatening typhoid and paratyphoid fevers.201020511368
community-acquired bloodstream infections in africa: a systematic review and meta-analysis.data on the prevalence and causes of community-acquired bloodstream infections in africa are scarce. we searched three databases for studies that prospectively studied patients admitted to hospital with at least a blood culture, and found 22 eligible studies describing 58 296 patients, of whom 2051 (13.5%) of 15 166 adults and 3527 (8.2%) of 43 130 children had bloodstream infections. 1643 (29.1%) non-malaria bloodstream infections were due to salmonella enterica (58.4% of these non-typhoidal sa ...201020510282
changing antibiotic sensitivity pattern and scope of chloramphenicol in the management of hospitalised patients of typhoid fever.enteric fever is a global health problem and there is emerging drug resistance with some reports of re-emerging sensitivity to previously used antibiotics eg, chloramphenicol. this study was done to compare the drug sensitivity pattern of enteric fever over a decade period. twenty-five culture positive patients for s typhi from one study done between october 1993 to february 1995 and 35 positive patients from another study done between september 2005 and august 2006 in the same hospital were tak ...200920509470
identifying the role of flagella in the transport of motile and nonmotile salmonella enterica serovars.the influence of bacterial flagella on cell transport has been examined using three salmonella enterica serovars with different motility phenotypes. both a packed bed (pb) column and radial stagnation point flow system (rspf) were employed to investigate bacterial deposition kinetics onto quartz over a range of conditions. the aim of this study was to better understand the role of motility on the deposition behavior of salmonella, an important food- and waterborne pathogen. in both the pb column ...201020504046
visible fluorescence detection of type iii protein secretion from bacterial pathogens.type iii protein secretion is essential for many gram-negative bacterial infections of host cells and an attractive target for new antibacterial drugs. here, we describe a bacterial protein effector-carboxypeptidase g2 (cpg2) reporter system for fluorescence and visible detection of type iii protein secretion in salmonella typhimurium. this system provides a general method for measuring protein expression and secretion as well as a high-throughput and quantitative assay for analyzing type iii pr ...201020504019
structural and genetic characterization of the closely related o-antigens of escherichia coli o85 and salmonella enterica o17.o-antigen is a part of the lipopolysaccharide present in the outer membrane of gram-negative bacteria, which confers major antigenic variability to the cell surface. in this study, we report on a previously undefined pair of escherichia coli and salmonella enterica with closely related o-antigens. the o-polysaccharides were isolated from the lipopolysaccharides of e. coli o85 and s. enterica o17 by mild acid degradation and studied by sugar analysis and nmr spectroscopy. the following structure ...201120501518
contemporary formulation and distribution practices for cold-filled acid products: australian industry survey and modeling of published pathogen inactivation data.a survey of 12 australian manufacturers indicated that mild-tasting acids and preservatives are used to partially replace acetic acid in cold-filled acid dressings and sauces. in contrast to traditional ambient temperature distribution practices, some manufacturers indicated that they supply the food service sector with cold-filled acid products prechilled for incorporation into ready-to-eat foods. the comité des industries des mayonnaises et sauces condimentaires de la communauté economique eur ...201020501041
intra-abdominal pseudocyst and abscess formation caused by non-typhoid salmonella. 201020500481
chicken tlr21 is an innate cpg dna receptor distinct from mammalian tlr9.tlrs comprise a family of evolutionary conserved sensory receptors that respond to distinct classes of ligands. for one major evolutionary branch of tlrs, the ligands are still largely unknown. here we report the cloning and function of one member of this group, chicken tlr21 (chtlr21). this tlr is absent in the human species but has homologs in fish and frog and displays similarity with mouse tlr13. expression of chtlr21 in hek293 cells resulted in activation of nf-kappab in response to unmethy ...201020498358
in vitro activity of azithromycin against nontyphoidal salmonella enterica.the in vitro activity of azithromycin against 1,237 nontyphoidal salmonella enterica isolates collected from finnish patients between 2003 and 2008 was investigated. only 24 (1.9%) of the isolates tested and 15 (5.1%) of the 294 isolates with reduced fluoroquinolone susceptibility had azithromycin mics of >or=32 microg/ml. these data show that azithromycin has good in vitro activity against nontyphoidal s. enterica, and thus, it may be a good candidate for clinical treatment studies of salmonell ...201020498312
salmonella enterica serovar enteritidis tatb and tatc mutants are impaired in caco-2 cell invasion in vitro and show reduced systemic spread in chickens.salmonella enterica subsp. enterica serovar enteritidis is a leading causative agent of gastroenteritis in humans. this pathogen also colonizes the intestinal tracts of poultry and can spread systemically in chickens. transfer to humans usually occurs through undercooked or improperly handled poultry meat or eggs. the bacterial twin-arginine transport (tat) pathway is responsible for the translocation of folded proteins across the cytoplasmic membrane. in order to study the role of the tat syste ...201020498258
antimicrobial potential of polyphenols extracted from almond skins.to evaluate the antimicrobial properties of flavonoid-rich fractions derived from natural and blanched almond skins, the latter being a by-product from the almond processing industry.201020497495
toll-like receptors 1 and 2 cooperatively mediate immune responses to curli, a common amyloid from enterobacterial biofilms.responses to host amyloids and curli amyloid fibrils of escherichia coli and salmonella enterica serotype typhimurium are mediated through toll-like receptor (tlr) 2. here we show that tlr2 alone was not sufficient for mediating responses to curli. instead, transfection experiments with human cervical cancer (hela) cells and antibody-mediated inhibition of tlr signalling in human macrophage-like (thp-1) cells suggested that tlr2 interacts with tlr1 to recognize curli amyloid fibrils. tlr1/tlr2 a ...201020497180
tolc dependency of multidrug efflux systems in salmonella enterica serovar typhimurium.tolc is a major outer membrane channel and it plays an important role in the excretion of a wide range of molecules, including antibiotics. a recent study has shown that salmonella enterica serovar typhimurium has nine functional drug efflux pumps; however, the tolc dependency of these efflux pumps remains to be studied in detail. the aim of this study was to investigate the tolc dependency of multidrug efflux pumps in this organism.201020495209
comparative virulence genotyping and antimicrobial susceptibility profiling of environmental and clinical salmonella enterica from cochin, india.salmonella enterica serotype newport is an important cause of non-typhoidal salmonellosis, a clinically less severe infection than typhoid fever caused by s. enterica serotype typhi. in this investigation, the virulence genotypes of s. enterica newport isolated from a backwater environment were compared with salmonella typhi from clinical cases in the same region where salmonellosis is endemic. genotyping was done by pcr screening for virulence markers associated with salmonella pathogenicity is ...201120490498
microbiology. salmonella's safety catch. 201020489009
salmonella enterica subspecies enterica serovar enteritidis salmonella pathogenicity island 2 type iii secretion system: role in intestinal colonization of chickens and systemic spread.salmonella enterica subspecies enterica serovar enteritidis (s. enteritidis) has been identified as a significant cause of salmonellosis in humans. salmonella pathogenicity islands 1 and 2 (spi-1 and spi-2) each encode a specialized type iii secretion system (t3ss) that enables salmonella to manipulate host cells at various stages of the invasion/infection process. for the purposes of our studies we used a chicken isolate of s. enteritidis (sal18). in one study, we orally co-challenged 35-day-ol ...201020488876
live attenuated salmonella enterica serovar typhimurium expressing swine interferon-alpha has antiviral activity and alleviates clinical signs induced by infection with transmissible gastroenteritis virus in piglets.enhancing innate and acquired immunity by cytokines such as ifn-alpha appears to be useful as a first line of defense against viral infection. however, the practical use of cytokines in livestock is not evident due to cost and production issues associated with mass administration. in this study, we tested the efficacy of live attenuated salmonella enterica serovar typhimurium designed to secrete swine ifn-alpha (swifn-alpha) protein for preventing the clinical signs caused by infection with tran ...201020488264
carbon metabolism of enterobacterial human pathogens growing in epithelial colorectal adenocarcinoma (caco-2) cells.analysis of the genome sequences of the major human bacterial pathogens has provided a large amount of information concerning their metabolic potential. however, our knowledge of the actual metabolic pathways and metabolite fluxes occurring in these pathogens under infection conditions is still limited. in this study, we analysed the intracellular carbon metabolism of enteroinvasive escherichia coli (eiec hn280 and eiec 4608-58) and salmonella enterica serovar typhimurium (stm 14028) replicating ...201020485672
prevalence of salmonella enterica in poultry and eggs in uruguay during an epidemic due to salmonella enterica serovar enteritidis.salmonella enterica serovar enteritidis (s. enteritidis) is frequently associated with food-borne disease worldwide. poultry-derived products are a major source. an epidemic of human infection with s. enteritidis occurred in uruguay, and to evaluate the extent of poultry contamination, we conducted a nationwide survey over 2 years that included the analysis of sera from 5,751 birds and 12,400 eggs. serological evidence of infection with salmonella group o:9 was found in 24.4% of the birds. all p ...201020484605
structural and genetic evidence for the close relationship between escherichia coli o71 and salmonella enterica o28 o-antigens.o-antigen is the most variable cell wall constituent of gram-negative bacteria. escherichia coli and salmonella enterica are closely related species. in this work, we present structural and genetic evidence for the close relationship between o-antigens of e. coli o71 and s. enterica o28. the e. coli o71 o-antigen was found to consist of tetrasaccharide-repeating units containing d-galpnac, d-galp, l-rhap, and d-quip3nac, with multiple o-acetyl lateral groups. it is very similar to the known stru ...201020482625
sorting nexin 3 (snx3) is a component of a tubular endosomal network induced by salmonella and involved in maturation of the salmonella-containing vacuole.salmonella enterica serovar typhimurium is an intracellular pathogen that grows within a modified endomembrane compartment, the salmonella-containing vacuole (scv). maturation of nascent scvs involves the recruitment of early endosome markers and the remodelling of phosphoinositides at the membrane of the vacuole, in particular the production of phosphatidylinositol 3-phosphate [pi(3)p]. sorting nexins (snxs) are a family of proteins characterized by the presence of a phox homology (px) domain t ...201020482551
in vitro efficacy of the combination of ciprofloxacin and cefotaxime against nalidixic acid-resistant salmonella enterica serotype typhi.typhoid fever is a systemic intracellular infection caused by salmonellaenterica serotype typhi. the emergence and spread of nalidixic acid-resistant s. typhi (narst) is challenging for clinicians in many countries owing to the lack of suitable treatment options. the aim of this study was to identify in vitro synergistic combinations of antibiotics against s. typhi. in vitro time-kill studies were performed on three clinical narst isolates and one type strain of nalidixic acid-susceptible s. typ ...201020478696
the effect of long-term propionate adaptation on the stress resistance of salmonella enteritidis.adaptive phenotypes of enteric bacterial pathogens in response to in vivo-mimicking stress conditions are important because of their potentiality to enhance stress resistance and ameliorate measures intended to control transmission and infectivity. salmonella enterica serotype enteritidis (s. enteritidis) encounters a variety of such environments throughout its infection cycle, including high concentrations of the short-chain fatty acid, propionate (pa), during food processing and within the gut ...201020477896
human infection with salmonella choleraesuis in hong kong. 196720475876
a salmonella enterica serovar typhi plasmid induces rapid and massive apoptosis in infected macrophages.pr(st98) is a chimeric plasmid isolated from salmonella enterica serovar typhi (s. typhi) that mediates the functions of drug resistance and virulence. previously, we reported that salmonella plasmid virulence (spv) genes were present in s. typhi. in our current study, we investigated whether plasmid pr(st98) exhibits significant cytotoxicity in macrophages. pr(st98) was transferred into the avirulent salmonella enterica serovar typhimurium (s. typhimurium) strain ria to create the transconjugan ...201020473323
down-regulation of the escherichia coli k-12 nrf promoter by binding of the nsrr nitric oxide-sensing transcription repressor to an upstream site.fnr-dependent activation of the escherichia coli k-12 nrf promoter is downregulated by the nitric oxide-sensitive nsrr protein together with the nucleoid-associated protein ihf, which bind to overlapping targets adjacent to the dna site for fnr. the nsrr target is inactivated by mutation at the salmonella enterica serovar typhimurium nrf promoter.201020472787
pork contaminated with salmonella enterica serovar 4,[5],12:i:-, an emerging health risk for humans.salmonella enterica subsp. enterica serovar 4,[5],12:i:- is a monophasic variant of s. enterica serovar typhimurium (antigenic formula 4,[5],12:i:1,2). worldwide, especially in several european countries and the united states, it has been reported among the 10 most frequently isolated serovars in pigs and humans. in the study reported here, 148 strains of the monophasic serovar isolated from pigs, pork, and humans in 2006 and 2007 in germany were characterized by various phenotypic and genotypic ...201020472721
salmonellosis outbreak due to chicken contact leading to a foodborne outbreak associated with infected delicatessen workers.salmonella is the most common bacterial cause of foodborne outbreaks in the united states. starting in june 2007, investigation of a cluster of salmonella montevideo cases with indistinguishable pulsed-field gel electrophoresis (pfge) patterns resulted in the identification of an outbreak associated with contact with chickens purchased from a single hatchery. nine minnesota cases from may through august 2007 were part of this outbreak. cases with the outbreak pfge pattern of salmonella montevide ...201020470192
extended-spectrum beta-lactamase-producing salmonella enterica serovar oranienburg (ctx-m-2 group) in a pediatric hospital in tucumán, argentina.salmonella sp infections have been reported over recent years in hospitals in argentina and other countries due to multiresistant strains. the aim of this study was to characterize the extended-spectrum beta-lactamases in third-generation cephalosporin-resistant strains of salmonella enterica serovar oranienburg.201020464138
salmonella enterica serovar virchow bacteremia presenting as typhoid-like illness in an immunocompetent patient.we report a typhoid-like illness with fever and altered consciousness in a 22-year-old man with growth of salmonella enterica serovar virchow in blood and stool culture. bacteremia and invasive disease due to non-typhoid salmonellae (nts) are known in severely immunocompromised patients, but so far have not been described in immunocompetent adults.201020463163
experimental identification and characterization of 97 novel npcrna candidates in salmonella enterica serovar typhi.we experimentally identified and characterized 97 novel, non-protein-coding rna candidates (npcrnas) from the human pathogen salmonella enterica serovar typhi (hereafter referred to as s. typhi). three were specific to s. typhi, 22 were restricted to salmonella species and 33 were differentially expressed during s. typhi growth. we also identified salmonella pathogenicity island-derived npcrnas that might be involved in regulatory mechanisms of virulence, antibiotic resistance and pathogenic spe ...201020460466
direct and indirect transmission of four salmonella enterica serotypes in pigs.feed-borne spread of salmonella spp. to pigs has been documented several times in recent years in sweden. experiences from the field suggest that feed-associated serotypes might be less transmittable and subsequently easier to eradicate from pig herds than other serotypes more commonly associated to pigs. four salmonella serotypes were selected for experimental studies in pigs in order to study transmissibility and compare possible differences between feed-associated (s cubana and s yoruba) and ...201020459711
overexpression of the reca gene decreases oral but not intraperitoneal fitness of salmonella enterica.transcription of the salmonella enterica reca gene is negatively controlled by the lexa protein, the repressor of the sos response. the introduction of a mutation (recao6869) in the lexa binding site, in the promoter region of the s. enterica atcc 14028 reca gene, allowed the analysis of the effect that reca protein overproduction has on the fitness of this virulent strain. the fitness of orally but not intraperitoneally inoculated recao6869 cells decreased dramatically. however, the sos respons ...201020457791
the utility and application of real-time pcr and fish in the detection of single-copy gene targets in escherichia coli o157:h7 and salmonella typhimurium.the ultimate specificity in molecular-based assays for pathogen detection relies on the design of the primers and probes. their ability to hybridize to dna sequences found only in pathogens can be realized by designing primers and probes that are complementary to pathogen-specific virulence genes. this study evaluates the detection and enumeration strengths of real-time pcr (qpcr) and fluorescent in situ hybridization (fish) for selected waterborne pathogens and their ultimate applicability with ...201020453912
characterization of arsenic-resistant bacteria from the rhizosphere of arsenic hyperaccumulator pteris vittata.arsenic hyperaccumulator fern pteris vittata l. produces large amounts of root exudates that are hypothesized to solubilize arsenic and maintain a unique rhizosphere microbial community. total heterotrophic counts on rich or defined media supplemented with up to 400 mmol/l of arsenate showed a diverse arsenate-resistant microbial community from the rhizosphere of p. vittata growing in arsenic-contaminated sites. twelve bacterial isolates tolerating 400 mmol/l of arsenate in liquid culture were i ...201020453910
investigation of risk factors for salmonella on commercial egg-laying farms in great britain, 2004-2005.in 2004/05, all european union member states were required to carry out standardised prevalence surveys to establish the baseline prevalence of salmonella in commercial laying flocks. as part of the survey in great britain, additional data were collected from 380 of the enrolled laying hen holdings to investigate risk factors for salmonella at farm level. stratified, simple random sampling was used to select holdings from which dust and boot swab samples were collected and tested for salmonella ...201020453235
population impact of vi capsular polysaccharide vaccine.development of a safe and efficacious vaccine against typhoid fever has been the mainstay of enteric vaccinology since production of the first parenteral whole-cell typhoid vaccine in 1896. the new-generation vi polysaccharide single-dose injectable typhoid vaccine developed in the 1980s is widely used in countries where it is locally produced, such as china, and in travelers from industrialized countries to typhoid-endemic settings. however, vi vaccine use is limited to private practice at a sm ...201020450323
spread of a multiresistant ctx-m-9-producing salmonella enterica serotype virchow phage type 19 in spain.the purpose of this study was to survey salmonella enterica serotype virchow phage type 19 (s. virchow pt19) strains submitted to the spanish national reference laboratory for salmonella (snrls) from 2002 to 2006 in order to determine the rate type and genetic background of beta-lactam resistance and to further identify the associated resistances. ninety-nine s. virchow pt19 strains were analysed. antimicrobial susceptibility was determined by the disk diffusion method using mueller-hinton agar ...201020446012
expression and crystallization of sedsba, sedsbl and sesrga from salmonella enterica serovar typhimurium.pathogens require protein-folding enzymes to produce functional virulence determinants. these foldases include the dsb family of proteins, which catalyze oxidative folding in bacteria. bacterial disulfide catalytic processes have been well characterized in escherichia coli k-12 and these mechanisms have been extrapolated to other organisms. however, recent research indicates that the k-12 complement of dsb proteins is not common to all bacteria. importantly, many pathogenic bacteria have an exte ...201020445269
genome-wide analysis of the h-ns and sfh regulatory networks in salmonella typhimurium identifies a plasmid-encoded transcription silencing mechanism.the conjugative inchi1 plasmid psfr27 from shigella flexneri 2a strain 2457t encodes the sfh protein, a paralogue of the global transcriptional repressor h-ns. sfh allows psfr27 to be transmitted to new bacterial hosts with minimal impact on host fitness, providing a 'stealth' function whose molecular mechanism has yet to be determined. the impact of the sfh protein on the salmonella enterica serovar typhimurium transcriptome was assessed and binding sites for sfh in the salmonella typhimurium g ...201020444106
serotypes and antimicrobial resistance profiles of salmonella isolates from pigs at slaughter in kenya.salmonellosis is considered one of the most widespread food-borne zoonoses in industrialized as well as developing countries. the presence of salmonella in food animals at slaughter and the consequent cross-contamination of edible carcass tissues present a significant food safety hazard.201020440063
mutations in the lipid a deacylase pagl which release the enzyme from its latency affect the ability of pagl to interact with lipopolysaccharide in salmonella enterica serovar typhimurium.pagl, a lipid a deacylase, is unique in that it is latent in the outer membrane of salmonella enterica serovar typhimurium. several point mutations in the extracellular loops of pagl, which do not affect its enzymatic activity, release it from this latency. precipitation analysis revealed that latent wild-type pagl associated with lipopolysaccharide, but non-latent pagl mutants did not. in contrast, non-latent pagl mutants preferentially associated with some membrane proteins. precipitation anal ...201020438711
real-time pcr detection of salmonella species using a novel target: the outer membrane porin f gene (ompf).to evaluate the outer membrane porin f gene (ompf) for the specific detection of salmonella species by real-time pcr assay.201020438619
a case report of thrombocytopenia-associated multiple organ failure secondary to salmonella enterica serotype typhi infection in a pediatric patient: successful treatment with plasma exchange.a high proportion of the patients with salmonella enterica serotype typhi infection develop severe sepsis. the mortality rate is high despite aggressive antimicrobial therapy in these patients. the case of a 10-year-old boy who developed thrombocytopenia-associated multiple organ failure (tamof) secondary to s. typhi infection is reported. the patient did not respond to antimicrobial treatment, including ciprofloxacin, in addition to conventional supportive measures, so plasma exchange was perfo ...201020438547
surveillance system and rapid tracing of primary sources in food-borne outbreaks by salmonella spp. part ii: molecular characterisation of some strains of salmonella enterica serovars enteritidis and typhimurium.salmonella enterica serovars enteritidis and typhimurium are the serotypes most frequently isolated from human cases. traditional surveillance systems, based on serological characterisation and epidemiology, are not able to identify these common strains that cause outbreaks in humans. innovative techniques are therefore necessary to accurately characterise these serotypes and hence accelerate the identification of the primary sources. within a larger study, the goal of which was to develop an ac ...200520437381
electronic tongue for simultaneous detection of endotoxins and other contaminants of microbiological origin.endotoxins, also referred to as pyrogens, are lipopolysaccharides (lps) present in the outer membrane of gram-negative bacteria, and represent one of the most dangerous microbiological contaminants in water for hemodialysis and intravenous infusion. a method is presented for the simultaneous detection of endotoxins and other bacterial lysis contaminating species in purified water for parenteral formulations. the technique used is electrochemical impedance spectroscopy, with data interpretation u ...201020434900
the biofilm architecture of sixty opportunistic pathogens deciphered using a high throughput clsm method.this study proposes a high throughput method based on confocal laser scanning microscopy (clsm) combined with the use of 96-wells microtiter plates compatible with high resolution imaging for the study of biofilm formation and structure. as an illustration, the three-dimensional structures of biofilms formed by 60 opportunistic pathogens were thus observed and quantified. the results revealed the diversity of biofilm architectures. specific spatial arrangement such as the mushroom-like structure ...201020433880
infected subdural haematoma due to salmonella enterica serovar typhimurium in an adult.a 65-year-old man with a history of alcoholism and gastrectomy was diagnosed with an infected subdural haematoma due to salmonella enterica serovar typhimurium. he was successfully treated with surgical drainage and intravenous ceftriaxone. to our knowledge, there has been no detailed case report in english of infected subdural haematoma or subdural empyema due to salmonella typhimurium in adults.201020430901
genetic control of resistance to salmonellosis and to salmonella carrier-state in fowl: a review.salmonellosis is a frequent disease in poultry stocks, caused by several serotypes of the bacterial species salmonella enterica and sometimes transmitted to humans through the consumption of contaminated meat or eggs. symptom-free carriers of the bacteria contribute greatly to the propagation of the disease in poultry stocks. so far, several candidate genes and quantitative trait loci (qtl) for resistance to carrier state or to acute disease have been identified using artificial infection of s. ...201020429884
production and characterisation of monoclonal antibodies specific for escherichia coli o157:h7.seven monoclonal antibodies (mabs) specific for escherichia coli o157:h7, one of the major causes of haemorrhagic colitis in humans, were produced by immunising balb/c mice with the strain e. coli o157:h7. these monoclonal antibodies do not cross-react with other bacteria such as salmonella enterica serovar typhimurium, e. coli o14, e. coli jm109, s. enterica serovar enteritidis, s. panama, s. saintpaul, s. derby, s. muenchen, s. bredeney, s. hadar, yersinia enterocolitica, proteus vulgaris, shi ...200620429059
predation of salmonella enterica serovar typhimurium by the rumen protozoon entodinium caudatum studied in vitro by fluorescence emission.predation of bacteria by protozoa has important implications on rumen metabolism and bacterial populations. protozoa can also restrict the passage of pathogenic bacteria to the host's lower gastrointestinal tract. this work aimed to evaluate the predation by entodinium caudatum (ec) and the intraprotozoal survival of salmonella enterica serovar typhimurium. ec cells from a monofaunated sheep were incubated for up to 105 min with a s. enterica strain producing a green fluorescent protein. rumen f ...201020427163
detection, amplification & sequence homology of sodc in clinical isolates of salmonella sp.periplasmic copper and zinc superoxide dismutase (cu,zn-sod or sodc) is an important component of the antioxidant shield which protects bacteria from the phagocytic oxidative burst. cu,zn-sods protect gram-negative bacteria against oxygen damage which have also been shown to contribute to the pathogenicity of these bacterial species. we report the presence of sodc in drug resistant salmonella sp. isolated from patients suffering from enteric fever. further sodc was amplified, cloned into escheri ...201020424309
effect of translesion dna polymerases, endonucleases and rpos on mutation rates in salmonella typhimurium.it has been suggested that bacteria have evolved mechanisms to increase their mutation rate in response to various stresses and that the translesion dna polymerase pol iv under control of the lexa regulon and the alternative sigma factor rpos are involved in regulating this mutagenesis. here we examined in salmonella enterica serovar typhimurium lt2 the rates for four different types of mutations (rifampicin, nalidixic acid, and chlorate resistance and lac(+) reversion) during various growth con ...201020421601
structural insight into the regulatory mechanisms of interactions of the flagellar type iii chaperone flit with its binding partners.for self-assembly of the bacterial flagellum, most of the flagellar component proteins synthesized in the cytoplasm are exported by the flagellar type iii export apparatus to the growing, distal end. flagellar protein export is highly organized and well controlled in every step of the flagellar assembly process. flagellar-specific chaperones not only facilitate the export of their cognate proteins, as well as prevent their premature aggregation in the cytoplasm, but also play a role in fine-tuni ...201020421493
culture-independent quantification of salmonella enterica in carcass gauze swabs by flotation prior to real-time pcr.to facilitate quantitative risk assessment in the meat production chain, there is a need for culture-independent quantification methods. the aim of this study was to evaluate the use of flotation, a non-destructive sample preparation method based on traditional buoyant density centrifugation, for culture-independent quantification of intact salmonella in pig carcass gauze swabs (100 cm(2)) prior to quantitative pcr (qpcr). a novel approach was investigated, excluding the homogenization step prio ...201120417982
safety and quality of ready-to-eat dry fermented sausages subjected to e-beam radiation.the inactivation kinetics in the death of listeriainnocua ntc 11288 (more radioresistant than five different strains of listeriamonocytogenes) and salmonella enterica serovar enteritidis and s. enterica serovar typhimurium by e-beam irradiation has been studied in two types of vacuum-packed rte dry fermented sausages ("salchichon" and "chorizo") in order to optimize the sanitation treatment of these products. a treatment of 1.29kgy was calculated to reach the food safety objective (fso) accordin ...200920416723
high bacterial contamination of pig tonsils at slaughter.food-borne zoonoses have a major health impact in industrial countries. campylobacter spp., salmonella enterica, yersinia enterocolitica and listeria monocytogenes are high-risk food-borne zoonotic hazards in finishing pigs. the objectives of this work were (1) to study the isolation rate of pathogenic y. enterocolitica, salmonella spp., campylobacter spp. and l. monocytogenes in the tonsils and feces and (2) to determine the number of mesophilic aerobic bacteria (mab) and escherichia coli in th ...200920416719
antimicrobial effect of photodynamic therapy using a highly pure chlorin e6.the aim of the present study is to evaluate the antimicrobial effect of photodynamic therapy (pdt) using a highly pure chlorin e(6) (ce(6)), against various pathogenic bacteria. to examine the antimicrobial effect of ce(6)-mediated pdt against staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, and salmonella enterica serovar typhimurium, inhibition zone formation, cfu quantification, and bacterial viability were evaluated. inhibition zone analysis showed that ce(6)-mediated pdt is ...201020414708
a purified mutant hema protein from salmonella enterica serovar typhimurium lacks bound heme and is defective for heme-mediated regulation in vivo.archaea, plants, and most bacteria synthesize heme using the c5 pathway, in which the first committed step is catalyzed by the enzyme glutamyl-trna reductase (glutr or hema). in some cases, an overproduced and purified hema enzyme contains noncovalently bound heme. the enteric bacteria salmonella enterica and escherichia coli also synthesize heme by the c5 pathway, and the hema protein in these bacteria is regulated by proteolysis. the enzyme is unstable during normal growth due to the action of ...201020412302
evidence for a second genomic island conferring multidrug resistance in a clonal group of strains of salmonella enterica serovar typhimurium and its monophasic variant circulating in italy, denmark, and the united kingdom.during the 2000s, a new clonal group with resistances to ampicillin, streptomycin, sulfonamides, and tetracycline (assut) emerged in italy among strains of salmonella enterica serovar typhimurium and its monophasic variant, salmonella enterica subspecies enterica serovar 4,[5],12:i:-. the pulsenet europe database allowed us to identify assut strains of both s. typhimurium and its monophasic variant, isolated in denmark and the united kingdom, with the same or very closely related pulsed-field ge ...201020410351
cholinergic stimulation of the immune system protects against lethal infection by salmonella enterica serovar typhimurium.summary: the cholinergic nervous system has been demonstrated to attenuate the inflammatory response during sepsis via the inhibitory action of acetylcholine (ach) on macrophages. these findings were largely based on experimental sepsis models using endotoxin as the inducing agent. herein, however, we report that the specific inhibition of acetylcholinesterase (ache) renders animals more resistant to infection by a virulent strain of salmonella enterica serovar typhimurium, a gram-negative enter ...201020408892
isolation of salmonella enterica serovar enteritidis in blue-fronted amazon parrot (amazona aestiva).avian salmonellosis is a disease caused by bacteria of the genus salmonella that can cause three distinct diseases in birds: pullorum diseases, fowl typhoid, and paratyphoid infection. various wildlife species are susceptible to infections by salmonella, regardless of whether they live in captivity or freely in the wild. the present study verified the presence of salmonella enterica serovar enteritidis in three captive specimens of amazona aestiva. the study involved a total of 103 birds undergo ...201020408416
investigation of an outbreak of salmonella enterica subsp. enterica serovar hadar food illness in the abruzzi region of italy.a comparative study was made of 22 strains of salmonella hadar isolated from victims of an outbreak of food illness in the abruzzi region of italy in 2000 and 21 strains of the same serotype isolated from poultry meat and human stool samples in the abruzzi and molise regions between 2000 and 2001. the aim of the investigation was to provide an epidemiological interpretation of the food illness outbreak to establish the degree of similarity between the s. hadar strains isolated from victims of th ...200820405442
a fast and highly sensitive blood culture pcr method for clinical detection of salmonella enterica serovar typhi.salmonella typhi causes an estimated 21 million new cases of typhoid fever and 216,000 deaths every year. blood culture is currently the gold standard for diagnosis of typhoid fever, but it is time-consuming and takes several days for isolation and identification of causative organisms. it is then too late to initiate proper antibiotic therapy. serological tests have very low sensitivity and specificity, and no practical value in endemic areas. as early diagnosis of the disease and prompt treatm ...201020403166
measurements of heme levels and respiration rate in salmonella enterica serovar typhimurium.this chapter describes assays for the measurement of heme levels and the rate of respiration in bacteria. an assay of ala supplementation is described, in which the effect of exogenous ala in reversing sensitivity to hydrogen peroxide is an indication of a reduced flow through the heme biosynthesis pathway. a protocol for measurement of the relative amount of heme by a fluorescence assay, based on stripping the iron from the heme moiety, leaving a protoporphyrin molecule which fluoresces followi ...201020401589
assays of sensitivity of antibiotic-resistant bacteria to hydrogen peroxide and measurement of catalase activity.bacteria, in common with other organisms that take advantage of aerobic respiration, generate and accumulate reactive oxygen species (ros) that damage dna, fatty acids, and proteins. in addition, intracellular pathogens like salmonella enterica are exposed to an oxidate burst produced by host macrophages. the relative ability of aerobically growing bacteria to withstand oxidative stress and eliminate ros has a large impact of their fitness in vitro and in vivo. methods are described here to meas ...201020401588
characterization of antimicrobial resistant salmonella enterica serovars enteritidis and typhimurium isolates from animal and food in southern italy.in the last two decades, the emergence and spread of antimicrobial-resistant pathogens, among them salmonella, has become a serious health hazard worldwide, and specifically the high incidence of multidrug resistance has been encountered widely in many european countries. this study examines the antimicrobial susceptibility of salmonella enterica strains typhimurium and enteritidis isolated in campania and calabria region (southern italy) from animal and food of animal origin. the relationship o ...201020401533
salmonella enterica bacteraemia: a multi-national population-based cohort study.salmonella enterica is an important emerging cause of invasive infections worldwide. however, population-based data are limited. the objective of this study was to define the occurrence of s. enterica bacteremia in a large international population and to evaluate temporal and regional differences.201020398281
an antisense rna that governs the expression kinetics of a multifunctional virulence gene.genome-wide transcriptome analyses of several bacterial species have recently uncovered a hitherto unappreciated amount of antisense transcription. however, the physiological role, regulation and significance of such antisense transcripts are presently unclear. we now report the identification of a cis-encoded 1.2 kb long antisense rna - termed amgr - that is complementary to the mgtc portion of the mgtcbr polycistronic message from salmonella enterica. the mgtcbr mrna specifies the mgtc protein ...201020398218
transfer of enteric pathogens to successive habitats as part of microbial cycles.escherichia coli o157:h7 gfp and salmonella enterica typhimurium gfp passed through six successive habitats within a microbial cycle. pathogen cultures were introduced into cow dung or fodder. microscopically observed cells and cfus were monitored in fodder, dung, dung-soil mix, rhizosphere and phyllosphere of cress or oat plants grown in infested dung-soil mix, and in excrements of snails or mice fed with contaminated cress or oat shoots. both methods were sensitive enough to monitor cells and ...201020397013
a mutation in tdca attenuates the virulence of salmonella enterica serovar typhimurium.the salmonella tdc operon encodes enzymes belonging to a metabolic pathway that degrades l-serine and l-threonine. the upregulation of the tdc operon and increased virulence of salmonella grown under oxygen-limiting conditions prompted us to investigate the role of the tdc operon in the pathogenesis of salmonella typhimurium. a salmonella strain carrying a null mutation in tdca, which encodes the transcriptional activator of the tdc operon, was impaired in mice infected intraperitoneally with th ...201020396961
ph sensing by intracellular salmonella induces effector translocation.salmonella enterica is an important intracellular bacterial pathogen of humans and animals. it replicates within host-cell vacuoles by delivering virulence (effector) proteins through a vacuolar membrane pore made by the salmonella pathogenicity island 2 (spi-2) type iii secretion system (t3ss). t3ss assembly follows vacuole acidification, but when bacteria are grown at low ph, effector secretion is negligible. we found that effector secretion was activated at low ph from mutant strains lacking ...201020395475
increased detection of extended spectrum beta-lactamase producing salmonella enterica and escherichia coli isolates from poultry.to gain more information on the genetic basis of the rapid increase in the number of isolates exhibiting non-wild type minimum inhibitory concentrations (mics) for cefotaxime observed since 2003, beta-lactamase genes of 22 salmonella enterica and 22 escherichia coli isolates from broilers in 2006 showing this phenotype were characterized by miniaturized micro-array, pcr and dna-sequencing. presence and size of plasmids were determined by s1-digest pulsed-field gel electrophoresis and further cha ...201020395076
typhoid in kenya is associated with a dominant multidrug-resistant salmonella enterica serovar typhi haplotype that is also widespread in southeast asia.in sub-saharan africa, the burden of typhoid fever, caused by salmonella enterica serovar typhi, remains largely unknown, in part because of a lack of blood or bone marrow culture facilities. we characterized a total of 323 s. typhi isolates from outbreaks in kenya over the period 1988 to 2008 for antimicrobial susceptibilities and phylogenetic relationships using single-nucleotide polymorphism (snp) analysis. there was a dramatic increase in the number and percentage of multidrug-resistant (mdr ...201020392916
multidrug resistant salmonella enterica serovar typhi. 200920387369
study of matrix additives for sensitive analysis of lipid a by matrix-assisted laser desorption ionization mass spectrometry.matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has been widely used for structural characterization of bacterial endotoxins (lipid a). however, the mass spectrometric behavior of the lipid a molecule is highly dependent on the matrix. furthermore, this dependence is strongly linked to phosphorylation patterns. using lipid a from escherichia coli o116 as a model system, we have investigated the effects of different matrices and comatrix compounds on th ...201020382818
thiamine biosynthesis can be used to dissect metabolic integration.the emergence of systems biology has re-emphasized the advantages of understanding biological processes with a global perspective. one biological process amenable to global approaches is microbial metabolism. this review describes a model system that contributes to the goals of systems biology by experimentally defining metabolic integration found in a bacterial cell and thus providing data needed for implementation and interpretation of systems approaches. we have taken a largely unbiased in vi ...201020382023
development of a novel multiplex pcr for the detection and differentiation of salmonella enterica serovars typhi and paratyphi a.in this study, we developed a multiplex polymerase chain reaction (mpcr) assay for pan-salmonella detection as well as for specific detection of serovars typhi and paratyphi a. the assay detects members of the salmonella genus by amplifying the outer membrane protein c (ompc). the presence of either salmonella serovar typhi or paratyphi a is indicated by amplification of the putative regulatory protein gene sty4220, which is common to both serovars. further differentiation of the serovars was ac ...201020381608
rapid detection and identification of bacterial pathogens by using an atp bioluminescence immunoassay.rapid identification of viable bacterial contaminants in food products is important because of their potential to cause disease. this study examined a method for microbial detection by using a combined atp bioluminescence immunoassay. escherichia coli o157:h7 and salmonella enterica serovar typhimurium were selected as target organisms because of their implication in foodborne illness. various matrices containing the target cells were examined, including ground beef homogenate, apple juice, milk ...201020377965
use of ionic liquid-based extraction for recovery of salmonella typhimurium and listeria monocytogenes from food matrices.methods for rapid separation (<5 h) and concentration of bacteria based on solubilization of complex food matrices have been developed recently to facilitate rapid molecular detection methods. however, a major disadvantage of these protocols is the resulting lack of viability of the microorganisms under study due to extensive use of chemicals and enzymes, which can inhibit subsequent quantitative microbiological analyses. in this study, a new class of organic salts, ionic liquids, were used for ...201020377956
functional dissection of translocon proteins of the salmonella pathogenicity island 2-encoded type iii secretion system.type iii secretion systems (t3ss) are essential virulence factors of most gram-negative bacterial pathogens. t3ss deliver effector proteins directly into the cytoplasm of eukaryotic target cells and for this function, the insertion of a subset of t3ss proteins into the target cell membrane is important. these proteins form hetero-oligomeric pores acting as translocon for the delivery of effector proteins. salmonella enterica is a facultative intracellular pathogen that uses the salmonella pathog ...201020377892
safety and immunogenicity of salmonella typhimurium expressing c-terminal truncated human il-2 in a murine model.salmonella enterica serovar typhimurium preferentially colonizes tumors in vivo and has proven to be an effective biologic vector. the attenuated s. enterica typhimurium strain chi4550 was engineered to express truncated human interleukin-2 and renamed salpil2. previously, we observed that a single oral administration of salpil2 reduced tumor number and volume, while significantly increasing local and systemic natural killer (nk) cell populations in an experimental metastasis model. here we repo ...201020376175
oral vaccination with attenuated salmonella enterica strains encoding t-cell epitopes from tumor antigen ny-eso-1 induces specific cytotoxic t-lymphocyte responses.bacterial fimbriae can accept foreign peptides and display them on the cell surface. a highly efficient gene replacement method was used to generate peptide vaccines based on salmonella enterica serovar typhimurium sl3261. the t-cell epitopes (ny-eso-1 p157-165 and p157-167) from ny-eso-1, which is a promising target antigen in patients for the specific immune recognition of cancer, were incorporated into the gene encoding agfa (the major subunit protein of thin aggregative fimbriae of salmonell ...201020375244
proteins from latex of calotropis procera prevent septic shock due to lethal infection by salmonella enterica serovar typhimurium.the latex of calotropis procera has been used in traditional medicine to treat different inflammatory diseases. the anti-inflammatory activity of latex proteins (lp) has been well documented using different inflammatory models. in this work the anti-inflammatory protein fraction was evaluated in a true inflammatory process by inducing a lethal experimental infection in the murine model caused by salmonella enterica subsp. enterica serovar typhimurium.201020371281
antibacterial effect of the bioactive compound beauvericin produced by fusarium proliferatum on solid medium of wheat.to obtain the bioactive compound beauvericin (bea), fusarium proliferatum cect 20569 was grown on a solid medium of wheat, utilizing the technique of the solid state fermentation (ssf), being this mycotoxin purified by high performance liquid chromatography (hplc) with a reverse phase semi-preparative column using as the mobile phase acetonitrile/water in gradient condition. the purity of the bea was verified by analytical hplc and liquid chromatography tandem mass spectrometry (lc/ms-ms). the p ...201020371252
rck of salmonella enterica, subspecies enterica serovar enteritidis, mediates zipper-like internalization.salmonella can invade non-phagocytic cells through its type iii secretion system (t3ss-1), which induces a trigger entry process. this study showed that salmonella enterica, subspecies enterica serovar enteritidis can also invade cells via the rck outer membrane protein. rck was necessary and sufficient to enable non-invasive e. coli and rck-coated beads to adhere to and invade different cells. internalization analysis of latex beads coated with different rck peptides showed that the peptide con ...201020368731
salmonella enterica serovar typhimurium invades fibroblasts by multiple routes differing from the entry into epithelial cells.fibroblasts are ubiquitous cells essential to tissue homeostasis. despite their nonphagocytic nature, fibroblasts restrain replication of intracellular bacterial pathogens such as salmonella enterica serovar typhimurium. the extent to which the entry route of the pathogen determines this intracellular response is unknown. here, we analyzed s. typhimurium invasion in fibroblasts obtained from diverse origins, including primary cultures and stable nontransformed cell lines derived from normal tiss ...201020368348
molecular epidemiology of ampicillin resistance in salmonella spp. and escherichia coli from wastewater and clinical specimens.molecular epidemiology at local scale in sicily (italy) of ampicillin resistance in salmonella spp. isolates from municipal wastewater (n = 64) and clinical specimens (n = 274) is described in comparison with previously examined escherichia coli isolates (n = 273) from wastewater. high prevalence of antibiotic resistance (28.9%) with highest resistance rates against ampicillin (22.7%) was observed in e. coli isolates. different resistance rates were observed in salmonella according to the serova ...201020367333
salmonellosis in garden birds in scotland, 1995 to 2008: geographic region, salmonella enterica phage type and bird species.salmonellosis was diagnosed in garden birds from 198 incidents in scotland between september 1995 and august 2008. salmonellosis was essentially a disease of finches in the north of scotland, but in the south of scotland it was also a problem in house sparrows. almost all of the incidents were caused by salmonella typhimurium phage types 40 or 56/variant, but regional variation in phage types was observed. in the north of scotland, one phage type (dt 40) predominated, but in the south of scotlan ...201020364008
antibiofilm activity of the marine bacterium pseudoalteromonas sp. strain 3j6.biofilm formation results in medical threats or economic losses and is therefore a major concern in a variety of domains. in two-species biofilms of marine bacteria grown under dynamic conditions, pseudoalteromonas sp. strain 3j6 formed mixed biofilms with bacillus sp. strain 4j6 but was largely predominant over paracoccus sp. strain 4m6 and vibrio sp. strain d01. the supernatant of pseudoalteromonas sp. 3j6 liquid culture (sn(3j6)) was devoid of antibacterial activity against free-living paraco ...201020363799
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