Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| lipopolysaccharide hyperpolarizes guinea pig airway epithelium by increasing the activities of the epithelial na(+) channel and the na(+)-k(+) pump. | earlier, we found that systemic administration of lipopolysaccharide (lps; 4 mg/kg) hyperpolarized the transepithelial potential difference (v(t)) of tracheal epithelium in the isolated, perfused trachea (ipt) of the guinea pig 18 h after injection. as well, lps increased the hyperpolarization component of the response to basolateral methacholine, and potentiated the epithelium-derived relaxing factor-mediated relaxation responses to hyperosmolar solutions applied to the apical membrane. we hypo ... | 2010 | 20639350 |
| the omptins of yersinia pestis and salmonella enterica cleave the reactive center loop of plasminogen activator inhibitor 1. | plasminogen activator inhibitor 1 (pai-1) is a serine protease inhibitor (serpin) and a key molecule that regulates fibrinolysis by inactivating human plasminogen activators. here we show that two important human pathogens, the plague bacterium yersinia pestis and the enteropathogen salmonella enterica serovar typhimurium, inactivate pai-1 by cleaving the r346-m347 bait peptide bond in the reactive center loop. no cleavage of pai-1 was detected with yersinia pseudotuberculosis, an oral/fecal pat ... | 2010 | 20639337 |
| in vivo selection of aac(6')-ib-cr and mutations in the gyra gene in a clinical qnrs1-positive salmonella enterica serovar typhimurium dt104b strain recovered after fluoroquinolone treatment. | to characterize the mechanisms implicated in the in vivo selection of quinolone and aminoglycoside resistance in a faecal salmonella enterica serovar typhimurium dt104b strain recovered after ciprofloxacin treatment of a hospitalized elderly patient with acute gastroenteritis. | 2010 | 20639314 |
| structural and genetic characterization of the o-antigen of salmonella enterica o56 containing a novel derivative of 4-amino-4,6-dideoxy-d-glucose. | based on the o-antigens (o-polysaccharides), one of the most variable cell constituents, 46 o-serogroups have been recognized in the kauffmann-white serotyping scheme for salmonella enterica. in this work, the structure of the o-polysaccharide and the genetic organization of the o-antigen gene cluster of s. enterica o56 were investigated. as judged by sugar and methylation analyses, along with nmr spectroscopic data, the o-polysaccharide has a linear tetrasaccharide o-unit, which consists of one ... | 2010 | 20638049 |
| dietary glycosaminoglycans interfere in bacterial adhesion and gliadin-induced pro-inflammatory response in intestinal epithelial (caco-2) cells. | dietary components may have an important role in maintaining a balanced gut microbiota composition. celiac disease is an autoimmune enteropathy caused by gliadins, and has been associated with a reduced proportion of bifidobacterium in gut microbiota. this study evaluates the influence of glycosaminoglycans (gags) on bacterial adhesion and their contribution in the gliadins-induced inflammatory response. the adhesion of potential probiotic (bifidobacterium longum cect 7347 and bifidobacterium bi ... | 2010 | 20637226 |
| functional analysis of an alpha-helical antimicrobial peptide derived from a novel mouse defensin-like gene. | gene-encoded antimicrobial peptides (amps) are an essential component of the innate immune system in many species. analysis of beta-defensin gene expression in mouse tissue using primers that were specific for conserved sequences located outside of the beta-defensin translated region identified a novel small gene. the novel gene had an open reading frame of 114bp and encoded a predicted protein of 37 amino acid residues. a search of the genome database revealed that the gene locus and the sequen ... | 2010 | 20637182 |
| giant splenic abscess due to salmonella enteritidis in a returning traveler. | we report the case of an immunocompetent traveler returning from morocco who presented with a giant splenic abscess, revealing an infection by salmonella enterica serovar enteritidis. | 2010 | 20636602 |
| curcumin increases the pathogenicity of salmonella enterica serovar typhimurium in murine model. | curcumin has gained immense importance for its vast therapeutic and prophylactic applications. contrary to this, our study reveals that it regulates the defense pathways of salmonella enterica serovar typhimurium (s. typhimurium) to enhance its pathogenicity. in a murine model of typhoid fever, we observed higher bacterial load in peyer's patches, mesenteric lymph node, spleen and liver, when infected with curcumin-treated salmonella. curcumin increased the resistance of s. typhimurium against a ... | 2010 | 20634977 |
| potential egg contamination by salmonella enterica serovar typhimurium definitive type 104 following experimental infection of pullets at the onset of lay. | the present study investigated the potential of salmonella enterica serovar typhimurium (salmonella typhimurium) definitive type 104 (dt104) to contaminate eggs in vivo. of 10 strains of salmonella typhimurium dt104, none caused egg contamination when hens were inoculated orally. three passages of the strains through recovery from the reproductive organs of intravenously infected laying hens resulted in no egg contamination after oral infection of the hens. feed and water withdrawal for 24 h at ... | 2010 | 20634517 |
| changes in fourier transform infrared spectra of salmonella enterica serovars typhimurium and enteritidis after adaptation to stressful growth conditions. | the effects of growth conditions (temperature in the range 10-45 degrees c, sodium chloride concentration in the range 0-4%, aerobic vs. anaerobic growth and acidification of the growth medium, up to ph 4.5) on the fourier transform infrared (ft-ir) spectra of salmonella enterica serovars typhimurium and enteritidis were studied using multivariate statistical methods (hierarchical cluster analysis and factor analysis). although all environmental factors tested affected s. typhimurium and s. ente ... | 2010 | 20633942 |
| a new pathway for the synthesis of α-ribazole-phosphate in listeria innocua. | the genomes of listeria spp. encode all but one of 25 enzymes required for the biosynthesis of adenosylcobalamin (adocbl; coenzyme b(12) ). notably, all listeria genomes lack cobt, the nicotinamide mononucleotide:5,6-dimethylbenzimidazole (dmb) phosphoribosyltransferase (ec 2.4.2.21) enzyme that synthesizes the unique α-linked nucleotide n(1) -(5-phospho-α-d-ribosyl)-dmb (α-ribazole-5'-p, α-rp), a precursor of adocbl. we have uncovered a new pathway for the synthesis of α-rp in listeria innocua ... | 2010 | 20633228 |
| poly(anhydride) nanoparticles act as active th1 adjuvants through toll-like receptor exploitation. | the mechanisms that underlie the potent th1-adjuvant capacity of poly(methyl vinyl ether-co-maleic anhydride) nanoparticles (nps) were investigated. traditionally, polymer nps have been considered delivery systems that promote a closer interaction between antigen and antigen-presenting cells (apcs). our results revealed that poly(anhydride) nps also act as agonists of various toll-like receptors (tlrs) (tlr2, -4, and -5), triggering a th1-profile cytokine release (gamma interferon [ifn-gamma], 4 ... | 2010 | 20631332 |
| evaluation of pulsed-field gel electrophoresis profiles for identification of salmonella serotypes. | pulsed-field gel electrophoresis (pfge) is a standard typing method for isolates from salmonella outbreaks and epidemiological investigations. eight hundred sixty-six salmonella enterica isolates from eight serotypes, including heidelberg (n = 323), javiana (n = 200), typhimurium (n = 163), newport (n = 93), enteritidis (n = 45), dublin (n = 25), pullorum (n = 9), and choleraesuis (n = 8), were subjected to pfge, and their profiles were analyzed by random forest classification and compared to co ... | 2010 | 20631109 |
| assessing the cross contamination and transfer rates of salmonella enterica from chicken to lettuce under different food-handling scenarios. | cross contamination of foodborne pathogens from raw meats to ready-to-eat foods has caused a number of foodborne outbreaks. the cross contamination and transfer rates of salmonella enterica from chicken to lettuce under various food-handling scenarios were determined. the following scenarios were tested: in scenario 1, cutting board and knife used to cut chicken (10(6) cfu/g) were also used for cutting lettuce, without washing; in scenario 2, cutting board and knife were washed with water separa ... | 2010 | 20630321 |
| antimicrobial effects of wine: separating the role of polyphenols, ph, ethanol, and other wine components. | while the antimicrobial effectiveness of wine is well documented, relative contributions of the wine components to its antimicrobial activity is controversial. to separate the role of wine phenolics, ethanol, and ph from other wine constituents, the antimicrobial effects of intact wine were compared to that of phenols-stripped wine, dealcoholized wine, ethanol, and low ph applied separately and in combination, against 2 common foodborne pathogens, salmonella enterica serovar enteritidis and esch ... | 2010 | 20629891 |
| antioxidant and antibacterial activities on foodborne pathogens of artocarpus heterophyllus lam. (moraceae) leaves extracts. | total water extract, ethyl acetate, and aqueous fractions from the leaves of artocarpus heterophyllus were evaluated for phenolic content, antioxidant, and antibacterial activities against some foodborne pathogens such as e. coli, listeria monocytogenes, salmonella typhimurium, salmonella enterica, bacillus cereus, enterococcus faecalis, and staphylococcus aureus. the minimum inhibitory concentration (mics) of extract and fractions determined by the agar dilution method were ranged from 221.9 mi ... | 2010 | 20629886 |
| stability and antimicrobial activity of allyl isothiocyanate during long-term storage in an oil-in-water emulsion. | this study investigated the stability and antimicrobial activity of allyl isothiocyanate (aitc) in medium chain triglyceride (mct) or soybean oil (sbo) dispersed in an oil-in-water (o/w) system during long-term storage. oil type, content, and oxidative stability affect the stability and antimicrobial activity of aitc during storage. high oil content is favorable for aitc stability in the emulsion. notably, aitc with mct is more stable than aitc with sbo with the same oil content. consequently, a ... | 2010 | 20629866 |
| a novel host-specific restriction system associated with dna backbone s-modification in salmonella. | a novel, site-specific, dna backbone s-modification (phosphorothioation) has been discovered, but its in vivo function(s) have remained obscure. here, we report that the enteropathogenic salmonella enterica serovar cerro 87, which possesses s-modified dna, restricts dna isolated from escherichia coli, while protecting its own dna by site-specific phosphorothioation. a cloned 15-kb gene cluster from s. enterica conferred both host-specific restriction and dna s-modification on e. coli. mutational ... | 2010 | 20627870 |
| essential genes from arctic bacteria used to construct stable, temperature-sensitive bacterial vaccines. | all bacteria share a set of evolutionarily conserved essential genes that encode products that are required for viability. the great diversity of environments that bacteria inhabit, including environments at extreme temperatures, place adaptive pressure on essential genes. we sought to use this evolutionary diversity of essential genes to engineer bacterial pathogens to be stably temperature-sensitive, and thus useful as live vaccines. we isolated essential genes from bacteria found in the arcti ... | 2010 | 20624965 |
| evaluation of eight different cephalosporins for detection of cephalosporin resistance in salmonella enterica and escherichia coli. | this study evaluates the efficacy of eight different cephalosporins for detection of cephalosporin resistance mediated by extended spectrum beta-lactamases (esbl) and plasmidic ampc beta-lactamases in salmonella and escherichia coli. a total of 138 e. coli and 86 salmonella isolates with known beta-lactamase genes were tested for susceptibility toward cefoperazone, cefotaxime, cefpodoxime, cefquinome, ceftazidime, ceftiofur, ceftriaxone, and cefuroxime using minimum inhibitory concentration dete ... | 2010 | 20624078 |
| characterization of drug resistant salmonella enterica serotype typhimurium by antibiograms, plasmids, integrons, resistance genes and pfge. | forty-seven salmonella typhimurium (33 zoonotic, 14 clinical) strains were tested for antimicrobial resistance using the standard disk diffusion method. presence of relevant resistance genes and class 1 integrons were investigated by using pcr. pulsed-field gel electrophoresis (pfge) and plasmid profiling were carried out to determine the genomic diversity of salmonella typhimurium. approximately 57.4% of s. typhimurium were multidrug resistant (mdr) and showed high resistance rates to tetracycl ... | 2010 | 20622506 |
| oral administration of heat-killed lactobacillus plantarum strain b240 protected mice against salmonella enterica serovar typhimurium. | the purpose of this study was to investigate the effects of heat-killed lactobacillus plantarum strain b240 (b240) on systemic infection by salmonella enterica serovar typhimurium (s. typhimurium) and to determine the mechanism by which b240 protects against infection. mice were administered either b240 or saline orally for 3 weeks, and then inoculated with s. typhimurium. the mice treated with b240 were significantly protected against s. typhimurium as compared to those fed saline. moreover, tr ... | 2010 | 20622449 |
| comparison of salmonella serovar isolation and antimicrobial resistance patterns from porcine samples between 2003 and 2008. | food-borne salmonella infections can produce symptoms from mild gastroenteritis to severe systemic disease and death, representing an important public health issue in u.s. livestock and livestock products, which have been implicated as frequent sources of salmonella contamination. concerns have been raised about the spread of antibiotic resistance in salmonella strains, particularly those that originate from food animal sources, as a result of prophylactic and therapeutic antimicrobial use in th ... | 2010 | 20622228 |
| development of microarray and multiplex polymerase chain reaction assays for identification of serovars and virulence genes in salmonella enterica of human or animal origin. | salmonella enterica is an important enteric pathogen consisting of many serovars that can cause severe clinical diseases in animals and humans. rapid identification of salmonella isolates is especially important for epidemiologic monitoring and controlling outbreaks of disease. although immunologic and dna-based serovar identification methods are available for rapid identification of isolates, they are time consuming or costly or both. in the current study, 2 molecular methods for identification ... | 2010 | 20622226 |
| human complement factor h binds to outer membrane protein rck of salmonella. | serum resistance, or resistance to complement-mediated killing, is a key virulence property of microbial pathogens. rck is a 17-kda outer membrane protein encoded on the virulence plasmid of salmonella enterica serovars typhimurium and enteritidis. when expressed in either escherichia coli or s. enterica typhimurium, rck confers serum resistance independent of lps length. recently, the rck homolog from yersinia enterocolitica, ail, has been shown to bind the complement regulatory protein factor ... | 2010 | 20622116 |
| salmonella rubislaw gastroenteritis linked to a pet lizard. | 2010 | 20618116 | |
| effect of subinhibitory concentrations of plant-derived molecules in increasing the sensitivity of multidrug-resistant salmonella enterica serovar typhimurium dt104 to antibiotics. | this study investigated the efficacy of plant-derived antimicrobials, namely, trans-cinnamaldehyde, β-resorcylic acid, carvacrol, thymol, and eugenol or their combination, in increasing the sensitivity of salmonella typhimurium dt104 to five antibiotics. the subinhibitory concentrations of each antimicrobial or their combination containing concentrations lower than the individual subinhibitory concentrations were added to tryptic soy broth supplemented with antibiotics at their respective break ... | 2010 | 20618084 |
| rsf1010-like plasmids in australian salmonella enterica serovar typhimurium and origin of their sul2-stra-strb antibiotic resistance gene cluster. | salmonella enterica serovar typhimurium phage type 9 isolates resistant to streptomycin and sulfonamide have been recovered from both bovine and human sources in australia. this study aimed to identify the resistance genes and their location. polymerase chain reaction was used to screen for resistance genes and sul2 (sulphonamide resistance) and stra and strb (streptomycin resistance) were detected. a small streptomycin and sulfonamide resistance plasmid carrying the three resistance genes was r ... | 2010 | 20617928 |
| attachment and colonization by escherichia coli o157:h7, listeria monocytogenes, salmonella enterica subsp. enterica serovar typhimurium, and staphylococcus aureus on stone fruit surfaces and survival through a simulated commercial export chain. | the ability of the foodborne pathogens escherichia coli o157:h7, listeria monocytogenes, salmonella enterica subsp. enterica serovar typhimurium, and staphylococcus aureus to attach, colonize, and survive on stone fruit surfaces was investigated. fifty microliters of bacterial suspension was spot inoculated onto the sterile intact fructoplane of whole peaches and plums. minimum time required for initial adhesion and attachment was recorded for different surface contact times. surface colonizatio ... | 2010 | 20615337 |
| 6-hydroxydopamine-mediated release of norepinephrine increases faecal excretion of salmonella enterica serovar typhimurium in pigs. | salmonella enterica serovar typhimurium is an animal and zoonotic pathogen of worldwide importance. in pigs, transport and social stress are associated with reactivation and spread of salmonella typhimurium infection. the stress-related catecholamine norepinephrine (ne) has been reported to activate growth and virulence factor expression in salmonella; however the extent to which ne contributes to stress-associated salmonellosis is unclear. we studied the impact of releasing ne from endogenous s ... | 2010 | 20609329 |
| vaccination with attenuated salmonella enterica dublin expressing e coli o157:h7 outer membrane protein intimin induces transient reduction of fecal shedding of e coli o157:h7 in cattle. | escherichia coli serogroup o157:h7 has emerged as an important zoonotic bacterial pathogen, causing a range of symptoms from self-limiting bloody diarrhea to severe hemorrhagic colitis and hemolytic-uremic syndrome in humans. beef and dairy cattle are considered the most important animal reservoirs for this pathogen. one of the important virulence characteristics of e. coli o157:h7 is the eaea gene encoding the 97 kda surface protein intimin. intimin is required for attachment and effacement dur ... | 2010 | 20609252 |
| the ubiquitin c-terminal hydrolase uch-l1 promotes bacterial invasion by altering the dynamics of the actin cytoskeleton. | invasion of eukaryotic target cells by pathogenic bacteria requires extensive remodelling of the membrane and actin cytoskeleton. here we show that the remodelling process is regulated by the ubiquitin c-terminal hydrolase uch-l1 that promotes the invasion of epithelial cells by listeria monocytogenes and salmonella enterica. knockdown of uch-l1 reduced the uptake of both bacteria, while expression of the catalytically active enzyme promoted efficient internalization in the uch-l1-negative hela ... | 2010 | 20608941 |
| influence of salmonella enterica serovar typhimurium infection on intestinal goblet cells and villous morphology in broiler chicks. | live broiler chickens are important in the transmission of salmonella to humans. reducing salmonella levels in the intestine of broiler chickens, in part, requires understanding of the interactions between salmonella and the intestinal barriers that represent the first line of defense. such barriers include the mucus layer (composed of mucins secreted by goblet cells) and the underlying epithelium. two experiments were conducted to evaluate the effect of salmonella typhimurium infection on intes ... | 2010 | 20608528 |
| trends in salmonella enteric serovar typhi in nairobi, kenya from 2004 to 2006. | typhoid fever is a global health problem. the world health organization (who) estimates that the current annual global burden of typhoid is approximately 22 million new cases, 5% of which are fatal. | 2010 | 20601792 |
| evaluation of antimicrobial activity of probiotic bacteria against salmonella enterica subsp. enterica serovar typhimurium 1344 in a common medium under different environmental conditions. | the importance of probiotics in human nutrition has been gaining recognition in recent years. these organisms have been shown to promote human health by enhancing immunological and digestive functions and fighting respiratory tract infections. we propose an improved in vitro model for the study of probiotic antimicrobial activity against enteropathogens, by attempting to re-create, in a common culture medium, environmental growth conditions comparable to those present in the small intestine. a p ... | 2010 | 20600855 |
| evaluation of the humoral immune response in mice orally vaccinated with live recombinant attenuated salmonella enterica delivering a secreted form of yersinia pestis psaa. | yersinia pestis psaa is an adhesin that is synthesized inside macrophages. here, we evaluated the immune profile of codon-optimized y. pestis psaa synthesized in a live recombinant attenuated salmonella vaccine (rasv) strain chi9558. oral immunization of balb/c mice with chi9558(pya3705) delivering a secreted form of psaa, elicited a systemic psaa-specific immunoglobulin g (igg) response but offered limited protection against lethal challenge with the intranasally introduced y. pestis co92 strai ... | 2010 | 20600475 |
| positive autoregulation shapes response timing and intensity in two-component signal transduction systems. | positive feedback loops are regulatory elements that can modulate expression output, kinetics and noise in genetic circuits. transcriptional regulators participating in such loops are often expressed from two promoters, one constitutive and one autoregulated. here, we investigate the interplay of promoter strengths and the intensity of the stimulus activating the transcriptional regulator in defining the output of a positively autoregulated genetic circuit. using a mathematical model of two-comp ... | 2010 | 20600106 |
| regulated delayed expression of rfc enhances the immunogenicity and protective efficacy of a heterologous antigen delivered by live attenuated salmonella enterica vaccines. | the salmonella rfc gene encodes the o-antigen polymerase. we constructed three strains in which we replaced the native rfc promoter with the arabinose-dependent arac p(bad) promoter so that rfc expression was dependent on exogenously supplied arabinose provided during in vitro growth. the three mutant strains were designed to synthesize different amounts of rfc by altering the ribosome-binding sequence and start codon. we examined these strains for a number of in vitro characteristics compared t ... | 2010 | 20599580 |
| fine mapping of the chicken salmonellosis resistance locus (sal1). | salmonella enterica serovar typhimurium is a gram-negative bacterium that has a significant impact on both human and animal health. it is one of the most common food-borne pathogens responsible for a self-limiting gastroenteritis in humans and a similar disease in pigs, cattle and chickens. in contrast, intravenous challenge with s. typhimurium provides a valuable model for systemic infection, often causing a typhoid-like infection, with bacterial replication resulting in the destruction of the ... | 2009 | 20597881 |
| complete genome sequence of the incompatibility group i1 plasmid r64. | a streptomycin and tetracycline resistance plasmid r64 isolated from salmonella enterica serovar typhimurium belongs to the incompatibility group i1 (inci1). the dna sequence of the r64 conjugative transfer region was described previously (komano et al., 2000). here, we report the complete genome sequence of r64. in the circular double-stranded r64 genome with 120,826bp, 126 complete orfs are predicted. in addition, 2 and 6 different kinds of proteins are produced by translational reinitiation a ... | 2010 | 20594994 |
| the o-antigen of salmonella enterica o13 and its relation to the o-antigen of escherichia coli o127. | the following structure of the o-polysaccharide (o-antigen) of salmonella enterica o13 was established by chemical analyses along with 2d (1)h and (13)c nmr spectroscopy: -->2)-alpha-l-fucp-(1-->2)-beta-d-galp-(1-->3)-alpha-d-galpnac-(1-->3)-alpha-d-glcpnac-(1--> the o-antigen of s. enterica o13 was found to be closely related to that of escherichia coli o127, which differs only in the presence of a galnac residue in place of the glcnac residue and o-acetylation. the location of the o-acetyl gro ... | 2010 | 20594547 |
| salmonella-regulated lipopolysaccharide modifications. | salmonella enterica are gram-negative enteric pathogens that cause typhoid fever and gastroenteritis in humans. many bacteria, including salmonella, use signal transduction cascades such as two-component regulatory systems to detect and respond to stimuli in the local microenvironment. during infection, environmental sensing allows bacteria to regulate gene expression to evade host immune defenses and thrive in vivo. activation of the salmonella two-component regulatory systems phop-phoq and pmr ... | 2010 | 20593264 |
| ribonucleotide reductases of salmonella typhimurium: transcriptional regulation and differential role in pathogenesis. | ribonucleotide reductases (rnrs) are essential enzymes that carry out the de novo synthesis of deoxyribonucleotides by reducing ribonucleotides. there are three different classes of rnrs (i, ii and iii), all having different oxygen dependency and biochemical characteristics. salmonella enterica serovar typhimurium (s. typhimurium) harbors class ia, class ib and class iii rnrs in its genome. we have studied the transcriptional regulation of these three rnr classes in s. typhimurium as well as the ... | 2010 | 20593029 |
| functional analysis of the c-terminal domain of the wbap protein that mediates initiation of o antigen synthesis in salmonella enterica. | wbap catalyzes the transfer of galactose-1-phosphate onto undecaprenyl phosphate (und-p). the enzyme belongs to a large family of bacterial membrane proteins required for initiation of the synthesis of o antigen lipopolysaccharide and polysaccharide capsules. previous work in our laboratory demonstrated that the last transmembrane helix and c-terminal tail region of wbap (wbap(ct)) are sufficient for enzymatic activity. here, we demonstrate the cytoplasmic location of the wbap c-terminal tail an ... | 2010 | 20591829 |
| perceptions regarding workplace hazards at a veterinary teaching hospital. | objective: to assess perceptions of personnel working at a veterinary teaching hospital regarding risks of occupational hazards and compare those perceptions with assessments made by occupational safety experts. | 2010 | 20590501 |
| salmonella enterica enteritidis arthritis following trauma in a child with thalassemia major. | osteoarticular infections caused by non-typhi salmonella are exceptionally encountered. we report a case of a bacteriologically documented knee joint infection due to salmonella enterica serotype enteritidis, following trauma in a child with thalassemia major. emergency arthrotomy combined with antimicrobial therapy was helpful in eradication of infection. physicians should be aware of this rare manifestation of non-typhi salmonella infections in thalassemic patients. | 2010 | 20589479 |
| acc-1 beta-lactamase-producing salmonella enterica serovar typhi, india. | 2010 | 20587199 | |
| engineering of glycerol dehydrogenase for improved activity towards 1, 3-butanediol. | the objective of this study was to use protein engineering techniques to enhance the catalytic activity of glycerol dehydrogenase (glydh) on racemic 1, 3-butanediol (1, 3-bdo) for the bioproduction of the important pharmaceutical intermediate 4-hydroxy-2-butanone. three glydh genes (glda) from escherichia coli k-12, salmonella enterica, and klebsiella pneumoniae mgh78578 were shuffled to generate a random mutagenesis library. the nitroblue tetrazolium/phenazine methosulfate high throughput scree ... | 2010 | 20585771 |
| live recombinant salmonella typhi vaccines constructed to investigate the role of rpos in eliciting immunity to a heterologous antigen. | we hypothesized that the immunogenicity of live salmonella enterica serovar typhi vaccines expressing heterologous antigens depends, at least in part, on its rpos status. as part of our project to develop a recombinant attenuated s. typhi vaccine (rastyv) to prevent pneumococcal diseases in infants and children, we constructed three rastyv strains synthesizing the streptococcus pneumoniae surface protein pspa to test this hypothesis. each vector strain carried ten engineered mutations designed t ... | 2010 | 20585446 |
| plasmid-mediated resistance to cephalosporins in salmonella enterica serovar typhi. | this study characterized a cephalosporin-resistant salmonella enterica serovar typhi isolate. the organism possessed a plasmid encoding the ctx-m-15 extended-spectrum-beta-lactamase. this plasmid is the determinant for the phenotype of cephalosporin resistance and is transferrable among enterobacteriaceae. | 2010 | 20585124 |
| outbreak of salmonellosis caused by salmonella enterica serovar newport mdr-ampc in a large animal veterinary teaching hospital. | nosocomial salmonellosis is an important problem for large animal veterinary teaching hospitals (vths). | 2010 | 20584143 |
| salmonellae in food stuffs of plant origin and their implications on human health. | salmonella enterica is one of the most common causes of food-borne infection in human beings. cases of salmonella infection have been decreasing in europe in the last ten years, yet, salmonella infections are still the main cause of acute diarrhea syndrome. globalization has caused the international food industry to increase the production of collective nutrition produce and products. this has intensified the need for authorized and accredited laboratories to monitor microbiological food safety. ... | 2010 | 20582445 |
| transferable class 1 and 2 integrons in escherichia coli and salmonella enterica isolates of human and animal origin in lithuania. | antibiotic-resistant escherichia coli (n = 191) and salmonella enterica (n = 87) isolates of human and animal origin obtained in lithuania during 2005-2008 were characterized for the presence and diversity of class 1 and 2 integrons. e. coli isolates were obtained from patients with urinary tract infections (utis) (n = 59) and both healthy and diseased farm animals, including poultry (n = 54), swine (n = 35), and cattle (n = 43). isolates of non-typhoidal s. enterica were recovered from salmonel ... | 2010 | 20578916 |
| comparison of vi serology and nested pcr in diagnosis of chronic typhoid carriers in two different study populations in typhoid endemic area of india. | for detection of chronic typhoid carriers, nested pcr targeting flagellin the gene of salmonella enterica subspecies enterica serotype typhi was carried out on dna extracted from hepatobiliary specimens from 424 autopsies which were apparently free from gallbladder pathology on postmortem examination. the second study population was 508 healthy volunteers, who did not suffer from typhoid fever during the preceding year and whose sera were subjected to detection of carriage by estimation of vi an ... | 2010 | 20578552 |
| nationwide outbreak of salmonella enterica serotype 4,12:i:- infections in france, linked to dried pork sausage, march-may 2010. | in may 2010, a nationwide excess of infections with the specific monophasic variant salmonella enterica serotype 4,12:i:- was investigated in france. subtyping with multilocus variable number of tandem repeats analysis revealed a distinct epidemic strain within this excess. epidemiological investigations identified a dried pork sausage sold by a particular chain of supermarkets as the likely vehicle of transmission. the suspected batches have been withdrawn and recalled. | 2010 | 20576238 |
| interleukin-17a is required to suppress invasion of salmonella enterica serovar typhimurium to enteric mucosa. | salmonella enterica serovar typhimurium (s. typhimurium) causes a localized enteric infection and its elimination is dependent on a t helper type 1 immune response. however, the mechanism of the protective immune response against the pathogen in gut-associated lymphoid tissue (galt) at an early stage of the infection is not yet clarified. here, we show that interleukin-17a (il-17a) was constitutively expressed in galt; it was also detected on crypt and epithelial cells of the small intestine. ne ... | 2010 | 20575990 |
| enteric bacterial pathogens with zoonotic potential isolated from farm-raised deer. | the raising of captive white-tailed deer (odocoileus virginianus) is a growing agricultural industry in ohio as it is in several other areas of the united states and around the world. pooled fecal samples were collected from 30 white-tailed deer confinement facilities. samples were cultured for five enteric bacterial pathogens. premise prevalence rates were as follows: escherichia coli o157, 3.3%; listeria monocytogenes, 3.3%; salmonella enterica, 0%; yersinia enterocolitica, 30%; and clostridiu ... | 2010 | 20575673 |
| new approach to study the mechanism of antimicrobial protection of an active packaging. | this article reports on the antimicrobial efficiency of a new active packaging concept based on the use of two essential oils (cinnamon and oregano) and their chemical descriptors (cinnamaldehyde, thymol, and carvacrol) against the gram-positive bacterium listeria monocytogenes, the gram-negative bacterium salmonella choleraesuis, the yeast candida albicans, and the mold aspergillus flavus. complete inhibition of these microorganisms with either bactericidal or bacteriostatic effect has been dem ... | 2010 | 20575672 |
| glycomimicry: display of the gm3 sugar epitope on escherichia coli and salmonella enterica sv typhimurium. | oligosaccharides present on the surface of pathogenic bacteria play an important role in their interaction with their host. bacteria with altered cell surface structures can be used to study these interactions, and glycoengineering represents a tool to display a glycoepitope on a different bacterium. here, we present non-pathogenic escherichia coli and salmonella enterica serovar typhimurium expressing the sialyllactose oligosaccharide epitope of the ganglioside gm3. by expression of the galacto ... | 2010 | 20574043 |
| characterization of anti-salmonella enterica serotype typhi antibody responses in bacteremic bangladeshi patients by an immunoaffinity proteomics-based technology. | salmonella enterica serotype typhi is the cause of typhoid fever and a human-restricted pathogen. currently available typhoid vaccines provide 50 to 90% protection for 2 to 5 years, and available practical diagnostic assays to identify individuals with typhoid fever lack sensitivity and/or specificity. identifying immunogenic s. typhi antigens expressed during human infection could lead to improved diagnostic assays and vaccines. here we describe a platform immunoaffinity proteomics-based techno ... | 2010 | 20573880 |
| emergence and characterization of salmonella enterica serovar typhimurium phage type dt191a. | the emergence of a previously undefined phage type of salmonella enterica serovar typhimurium, designated dt191a, occurred in england and wales in july 2008. the new strain exhibits a number of distinctive phenotypic and genotypic features. this report provides the tools necessary to track s. typhimurium dt191a globally. | 2010 | 20573861 |
| development and evaluation of multilocus variable number tandem repeat analysis for fine typing and phylogenetic analysis of salmonella enterica serovar typhimurium. | we identified 16 variable number tandem repeat (vntr) loci for salmonella enterica serovar typhimurium. these vntrs were evaluated with panels of 183 diverse isolates, 203 closely related isolates and 54 isolates from seven outbreaks. the evaluations revealed that five of the 16 vntrs had diversity values greater than 0.5, and three (sttr5, sttr6 and sttr10) were hypervariable. the results obtained from the outbreak isolates suggested that the 16 vntrs were considerably stable in isolates recove ... | 2010 | 20573417 |
| cation selectivity by the cora mg2+ channel requires a fully hydrated cation. | the cora mg(2+) channel is the primary uptake system in about half of all bacteria and archaea. however, the basis for its mg(2+) selectivity is unknown. previous data suggested that cora binds a fully hydrated mg(2+) ion, unlike other ion channels. the crystal structure of thermotoga maritima cora shows a homopentamer with two transmembrane segments per monomer connected by a short periplasmic loop. this highly conserved loop, (281)efmpelkws(289) in salmonella enterica serovar typhimurium cora, ... | 2010 | 20568735 |
| chromosomally encoded blacmy-2 located on a novel sxt/r391-related integrating conjugative element in a proteus mirabilis clinical isolate. | integrating conjugative elements (ices) are mobile genetic elements that can transfer from the chromosome of a host to the chromosome of a new host through the process of excision, conjugation, and integration. although sxt/r391-related ices, originally demonstrated in vibrio cholerae o139 isolates, have become prevalent among v. cholerae isolates in asia, the prevalence of the ices among gram-negative bacteria other than vibrio spp. remains unknown. in addition, sxt/r391-related ices carrying g ... | 2010 | 20566768 |
| in vitro synergism of ciprofloxacin and cefotaxime against nalidixic acid-resistant salmonella enterica serotypes paratyphi a and paratyphi b. | paratyphoid fever is considered an emerging systemic intracellular infection caused by salmonella enterica serotypes paratyphi a, b, and c. we performed in vitro time-kill studies on three clinical isolates of nalidixic acid-resistant salmonella serotype paratyphi (narsp) with different concentrations of ciprofloxacin and cefotaxime to identify combinations of antibiotics with synergistic activity against paratyphoid fever. furthermore, we identify the frequency of mutations to ciprofloxacin, ce ... | 2010 | 20566759 |
| evaluation of the widal tube agglutination test for the diagnosis of typhoid fever among children admitted to a rural hdospital in tanzania and a comparison with previous studies. | the diagnosis of typhoid fever is confirmed by culture of salmonella enterica serotype typhi (s. typhi). however, a more rapid, simpler, and cheaper diagnostic method would be very useful especially in developing countries. the widal test is widely used in africa but little information exists about its reliability. | 2010 | 20565990 |
| [evaluation of premitest salmonella kit for identification of not-typable by conventional methods salmonella]. | despite the downward trend, salmonella is still one of the most important bacterial intestinal infections agents. for example, in 2007 y. in poland over 14 thousands human salmonelosis cases were notified, in 2008 y.--over 10 thousands. among all salmonella isolated from human source, most common (more then 80%) are two serotypes--s. enteritidis and s. typhimurium, but every year the number of non-typable (because of the lack i or ii phase flagellar antigens, autoagglutinative or non-motility pr ... | 2010 | 20564968 |
| cyclic di-gmp signaling regulates invasion by ehrlichia chaffeensis of human monocytes. | cyclic di-gmp (c-di-gmp) is a bacterial second messenger produced by ggdef domain-containing proteins. the genome of ehrlichia chaffeensis, an obligatory intracellular bacterium that causes human monocytic ehrlichiosis, encodes a single protein that contains a ggdef domain, called pled. in this study, we investigated the effects of c-di-gmp signaling on e. chaffeensis infection of the human monocytic cell line thp-1. recombinant e. chaffeensis pled showed diguanylate cyclase activity as it gener ... | 2010 | 20562302 |
| transcriptomic responses of salmonella enterica serovars enteritidis and typhimurium to chlorine-based oxidative stress. | salmonella enterica serovars enteritidis and typhimurium are the leading causative agents of salmonellosis in the united states. s. enteritidis is predominantly associated with contamination of shell eggs and egg products, whereas s. typhimurium is frequently linked to tainted poultry meats, fresh produce, and recently, peanut-based products. chlorine is an oxidative disinfectant commonly used in the food industry to sanitize the surfaces of foods and food processing facilities (e.g., shell eggs ... | 2010 | 20562293 |
| influence of temperature and predation on survival of salmonella enterica serovar typhimurium and expression of inva in soil and manure-amended soil. | the effects of three temperatures (5, 15, and 25 degrees c) on the survival of salmonella enterica serovar typhimurium in topsoil were investigated in small microcosms by three different techniques: plate counting, inva gene quantification, and inva mrna quantification. differences in survival were related to the effect of protozoan predation. tetracycline-resistant salmonella serovar typhimurium was inoculated into soil and manure-amended soil at 1.5 x 10(8) cells g soil(-1). population densiti ... | 2010 | 20562283 |
| substitution of the arginine/leucine residues in apidaecin ib with peptoid residues: effect on antimicrobial activity, cellular uptake, and proteolytic degradation. | several aspects of the mechanism of action of pro-rich antimicrobial peptides, together with their low toxicity in mammalian cells, make them good candidates for the development of new antibiotic agents. we investigated the effect induced in the insect antimicrobial peptide apidaecin ib by the replacement of a single arginine/leucine residue with a n-substituted glycine. the resulting peptoid-peptide hybrids are more resistant to proteolysis and devoid of any significant cytotoxic activity, but ... | 2009 | 20560644 |
| the cyclotide cycloviolacin o2 from viola odorata has potent bactericidal activity against gram-negative bacteria. | to determine the antibacterial activity of small cyclic plant proteins, i.e. cyclotides, and the importance of the surface exposed charged residues for activity. | 2010 | 20558471 |
| deletion of a prophage-like element causes attenuation of salmonella enterica serovar enteritidis and promotes protective immunity. | salmonella enterica serovar enteritidis (s. enteritidis) is a wide host range serovar belonging to the s. enterica genus. worldwide, it is one of the most frequent causes of food borne disease. similar to s. typhimurium, some virulence genes of s. enteritidis are located in pathogenicity islands and prophages. in this study we have generated a mutant strain of s. enteritidis lacking a prophage-like element, denominated varphise12. the resulting mutant strain was attenuated and promoted protectiv ... | 2010 | 20558245 |
| epidemiology and interaction of salmonella enterica serovar derby, infantis and typhimurium with porcine alveolar macrophages. | in this study we were interested in the serovars which are frequently isolated from pigs, i.e. s. typhimurium, s. derby and s. infantis. first we collected different isolates of s. infantis and s. derby and compared them by macrorestriction analysis. in the second part of the study we infected porcine alveolar macrophages (pams) with representative strains of these serovars and s. typhimurium and determined intracellular survival, cytotoxicity and cytokine response. in s. derby, 17 different pro ... | 2010 | 20554127 |
| antimicrobial properties of prunin, a citric flavanone glucoside, and its prunin 6″-o-lauroyl ester. | to determine the antimicrobial potential of prunin (p), a flavanone glucoside resulting from the hydrolysis of naringin present in grapefruit, and of its prunin 6″-o-lauroyl ester (pl), synthesized by enzymatic catalysis. | 2010 | 20553344 |
| salmonella enterica serovar typhi has a 4.1 kb genetic island inserted within the sapabcdf operon that causes loss of resistance to the antimicrobial peptide protamine. | to investigate the association between the presence of a genetic island inserted within the sapabcdf operon of salmonella typhi and the susceptibility to antimicrobial peptides. | 2010 | 20551214 |
| synthesis and antimicrobial activity of n,n'-bis(2-hydroxylbenzyl)-1,2-ethanediamine derivatives. | a series of n,n'-bis(2-hydroxylbenzyl)-1,2-ethanediamine derivatives and its schiff bases were synthesized, characterized and screened for in vitro antimicrobial activity against staphylococcus aureus, pseudomonas aeruginosa and salmonella enterica. result indicated that the ethylenediamine derivatives, n,n'-bis(2-hydroxy-5-bromobenzyl)-1,2-ethanediamine (21), and n,n'-bis(2-hydroxy-5-chlorobenzyl)-1,2-ethanediamine (22) showed the most favorable antimicrobial activity exhibiting lc(50) of 11.6 ... | 2010 | 20548968 |
| antimicrobial resistance and subtyping of salmonella enterica subspecies enterica serovar enteritidis isolated from human outbreaks and poultry in southern brazil. | to investigate antimicrobial resistance, 96 salmonella enterica subspecies enterica serovar enteritidis strains isolated from salmonellosis outbreaks and poultry-related products obtained in southern brazil were analyzed. macrorestriction patterns, obtained by pulsed-field gel electrophoresis and phage types, were assessed. although 43.75% of samples were sensitive to all drugs tested, resistance to sulfonamide (34.37%), trimethoprim-sulfamethoxazole (25.00%), nalidixic acid (14.58%), streptomyc ... | 2010 | 20548083 |
| isolation and characterization of a novel analyte from bacillus subtilis sc-8 antagonistic to bacillus cereus. | in this study, an effective substance was isolated from bacillus subtilis sc-8, which was obtained from traditionally fermented soybean paste, cheonggukjang. the substance was purified by hplc, and its properties were analyzed. it had an adequate antagonistic effect on bacilluscereus, and its spectrum of activity was narrow. when tested on several gram-negative and gram-positive foodborne pathogenic bacteria such as salmonella enterica, salmonella enteritidis, staphylococcus aureus, and listeria ... | 2010 | 20547349 |
| enterobacterial tumor colonization in mice depends on bacterial metabolism and macrophages but is independent of chemotaxis and motility. | despite promising results and increasing attention in bacterial cancer therapy, surprisingly little is known about initial tumor colonization and the interaction between bacteria and surrounding tumor tissue. here, we analyzed the role of chemotaxis, motility, and metabolism both in escherichia coli and salmonella enterica serovar typhimurium strains upon intravenous injection into tumor-bearing mice. in contrast to previous models, we found that chemotaxis and motility do not play a significant ... | 2010 | 20547100 |
| multiresistant salmonella enterica serovar 4,[5],12:i:- in europe: a new pandemic strain? | a marked increase in the prevalence of s. enterica serovar 4,[5],12:i:- with resistance to ampicillin, streptomycin, sulphonamides and tetracyclines (r-type assut) has been noted in food-borne infections and in pigs/pig meat in several european countries in the last ten years. one hundred and sixteen strains of s. enterica serovar 4,[5],12:i:- from humans, pigs and pig meat isolated in england and wales, france, germany, italy, poland, spain and the netherlands were further subtyped by phage typ ... | 2010 | 20546690 |
| unphosphorylated csgd controls biofilm formation in salmonella enterica serovar typhimurium. | the transcriptional regulator csgd of salmonella enterica serovar typhimurium (s. typhimurium) is a major regulator of biofilm formation required for the expression of csgba, which encodes curli fimbriae, and adra, coding for a diguanylate cyclase. csgd is a response regulator with an n-terminal receiver domain with a conserved aspartate (d59) as a putative target site for phosphorylation and a c-terminal luxr-like helix-turn-helix dna binding motif, but the mechanisms of target gene activation ... | 2010 | 20545866 |
| characterization of antimicrobial resistance of recent salmonella enterica serovar gallinarum isolates from chickens in south korea. | salmonella enterica serovar gallinarum isolates (n=105) from chickens in south korea between 2002 and 2007 were tested for antimicrobial susceptibility by determining minimum inhibitory concentrations of 16 antimicrobials, and their predominant resistance profiles were genetically characterized. most isolates (99/105; 94.3%) were resistant to nalidixic acid and resistant/intermediately resistant to fluoroquinolones, and 63.8% (67/105) of the isolates were resistant to three or more antimicrobial ... | 2010 | 20544426 |
| hemorrhagic colitis as the onset of wegener's granulomatosis. | 2011 | 20544207 | |
| specific and rapid enumeration of viable but nonculturable and viable-culturable gram-negative bacteria by using flow cytometry. | an issue of critical concern in microbiology is the ability to detect viable but nonculturable (vbnc) and viable-culturable (vc) cells by methods other than existing approaches. culture methods are selective and underestimate the real population, and other options (direct viable count and the double-staining method using epifluorescence microscopy and inhibitory substance-influenced molecular methods) are also biased and time-consuming. a rapid approach that reduces selectivity, decreases bias f ... | 2010 | 20543046 |
| the phosphoinositide phosphatase sopb manipulates membrane surface charge and trafficking of the salmonella-containing vacuole. | shifts in electrostatic surface charge of membranes have recently been highlighted as a significant factor contributing to protein targeting to the plasma membrane and nascent phagosomes. intracellular, vacuole-adapted pathogens may also regulate surface charge of their vacuoles to establish a replicative niche. since salmonella enterica serovar typhimurium controls trafficking of the salmonella-containing vacuole (scv) and inhibits its fusion with lysosomes, we investigated the contribution of ... | 2010 | 20542249 |
| bacteriophage tailspike proteins as molecular probes for sensitive and selective bacterial detection. | we report the use of genetically engineered tailspike proteins (tsps) from the p22 bacteriophage for the sensitive and selective detection of salmonella enterica serovar typhimurium. high yields of two mutant tsps, one with an n-terminal cysteine (n-cys) and another with a c-terminal cysteine (c-cys), have been obtained using recombinant protein expression and purification in escherichia coli. the mutant tsps did not have the native endorhamnosidase enzymatic activity of intact p22 phage as well ... | 2010 | 20541928 |
| prevalence of decreased susceptibility to triclosan in salmonella enterica isolates from animals and humans and association with multiple drug resistance. | previous laboratory studies have implicated triclosan as a possible selective force driving resistance to multiple antibiotics and have identified a number of triclosan resistance mechanisms in salmonella enterica. the aim of this work was to determine the prevalence of decreased susceptibility to triclosan in a panel of human and animal isolates of s. enterica and to identify the mechanisms of triclosan resistance in these strains. over 400 animal and human isolates of non-typhoidal salmonella ... | 2010 | 20541914 |
| structure and gene cluster of the o-antigen of salmonella enterica o60 containing 3-formamido-3,6-dideoxy-d-galactose. | an o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of salmonella enterica o60 strain g1462, and the following unique structure of the o-unit was determined by chemical analyses along with 2d (1)h and (13)c nmr spectroscopy: where fuc3nfo stands for 3-formamido-3,6-dideoxygalactose. the structure established is in agreement with the o-antigen gene cluster of s. enterica o60, which contains putative genes for the synthesis of gdp-d-man and dtdp-d-fuc3nfo, three gly ... | 2010 | 20538266 |
| effect of enrichment medium on real-time detection of salmonella enterica from lettuce and tomato enrichment cultures. | three enrichment broths commonly used for detection of salmonella (buffered peptone water [bpw], tryptic soy broth [tsb], and universal preenrichment broth [upb]) were compared for use in real-time sybr green pcr detection of salmonella introduced into enrichment cultures made from store-bought lettuce and tomatoes. the produce served as a source of normal plant microbiota to measure how well dna-based detection methods for salmonella work in a suspension of plant-associated bacteria that may be ... | 2010 | 20537259 |
| optical imaging of bacterial infection in living mice using deep-red fluorescent squaraine rotaxane probes. | two structurally related fluorescent imaging probes allow optical imaging of bacterial leg infection models in living athymic and immunocompetent mice. structurally, the probes are comprised of a deep-red fluorescent squaraine rotaxane scaffold with two appended bis(zinc(ii)-dicolylamine) (bis(zn-dpa)) targeting ligands. the bis(zn-dpa) ligands have high affinity for the anionic phospholipids and related biomolecules that reside within the bacterial envelope, and they are known to selectively ta ... | 2010 | 20536173 |
| [induction and migration of cryptic/defective salmonella enterica prophages as a consequence of infection with lytic phages is an additional factor in stability of a coevolutionary vector]. | the influence of infection of natural isolates of salmonella enterica with lytic (nonlysogenic) phages on the expression of resident cryptic or defective prophages in host bacteria was studied. the induction of defective/cryptic phages after infection with nonlysogenic phages and packaging of bacterial chromosomal fragments in capsids of defective phages is demonstrated. this may lead to migration and wide distribution of both the genomes of defective phages per se and various fragments of the b ... | 2010 | 20536029 |
| the epidemiology of extraintestinal non-typhoid salmonella in israel: the effects of patients' age and sex. | extraintestinal disease occurs in 5-8% of non-typhoid salmonella enterica (nts) infections and is more likely to be associated with hospitalization and death. the study examined the epidemiology of extraintestinal nts infections in israel and the possible effects of patients' age and sex. nts isolates passively submitted to the national salmonella reference center during 1996-2006 were the source for the study cohort. poisson regression models were used to assess incidence trends over the study ... | 2010 | 20535625 |
| recent advances in the field of salmonella typhi vaccines. | typhoid fever, caused by the bacterium salmonella enterica serovar typhi, remains a significant cause of human death and disease throughout large areas of the world. although there are currently three vaccines available against s. typhi they have a number of significant drawbacks which drive the development of much-needed new and improved versions. here recent advances towards such vaccines, from basic research to human trials, are discussed. | 2010 | 20534981 |
| copper homeostasis in salmonella is atypical and copper-cuep is a major periplasmic metal complex. | salmonella enterica sv. typhimurium (s. enterica sv. typhimurium) has two metal-transporting p(1)-type atpases whose actions largely overlap with respect to growth in elevated copper. mutants lacking both atpases over-accumulate copper relative to wild-type or either single mutant. such duplication of atpases is unusual in bacterial copper tolerance. both atpases are under the control of merr family metal-responsive transcriptional activators. analyses of periplasmic copper complexes identified ... | 2010 | 20534583 |
| phylogenetic incongruence arising from fragmented speciation in enteric bacteria. | evolutionary relationships among species are often assumed to be fundamentally unambiguous, where genes within a genome are thought to evolve in concert and phylogenetic incongruence between individual orthologs is attributed to idiosyncrasies in their evolution. we have identified substantial incongruence between the phylogenies of orthologous genes in escherichia, salmonella, and citrobacter, or e. coli, e. fergusonii, and e. albertii. the source of incongruence was inferred to be recombinatio ... | 2010 | 20534528 |
| mass spectrometry-based quantitative proteomic analysis of salmonella enterica serovar enteritidis protein expression upon exposure to hydrogen peroxide. | salmonella enterica, a common food-borne bacterial pathogen, is believed to change its protein expression profile in the presence of different environmental stress such as that caused by the exposure to hydrogen peroxide (h2o2), which can be generated by phagocytes during infection and represents an important antibacterial mechanism of host cells. among salmonella proteins, the effectors of salmonella pathogenicity island 1 and 2 (spi-1 and spi-2) are of particular interest since they are expres ... | 2010 | 20529336 |
| successful treatment of late salmonella infections in total hip replacement - report of two cases. | salmonella species can be rarely isolated from periprosthetic joint infections, however when present, are usually part of a severe septic clinical picture. | 2010 | 20529326 |
| tracking salmonella contamination in various watersheds and phenotypic and genotypic diversity. | salmonella enterica is an important foodborne pathogen, and contamination of surface and ground water that may result from various human activities, such as animal production and urbanization, may contribute to the public health burden. the aims of this study was to determine the sources of salmonella contamination in four different types of watersheds and to assess the relative contribution of multidrug-resistant strains. eighty-six water samples collected from four different watershed systems, ... | 2010 | 20528467 |
| expression of chicken leap-2 in the reproductive organs and embryos and in response to salmonella enterica infection. | in recent years host antimicrobial peptides and proteins have been recognised as key mediators of the innate immune response in many vertebrate species, providing the first line of defense against potential pathogens. in chickens a number of cationic antimicrobial peptides have been recently identified. however, although these peptides have been studied extensively in the avian gastrointestinal tract, little is known about their function in the chicken reproductive organs and embryos. chicken li ... | 2010 | 20526889 |