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identification and characterization of a new major histocompatibility complex class i gene in carp (cyprinus carpio l.).in this study we report the finding of three representatives of a new group of major histocompatibility complex class i sequences in carp: cyca-12 (cyca-ua1*01), a full-length cdna; cyca-sp1 (cyca-uaw1), a polymerase chain reaction (pcr) fragment from cdna; and cyca-g11 (cyca-ua1*02), a partial genomic clone. comparison of the amino acid sequences of cyca-12, cyca-sp1, and cyca-g11 with classical and non-classical class i sequences from other species shows considerable conservation in regions th ...19968613142
development of an in vitro model of essential fatty acid deficiency in fish cells.levels of eicosapentaenoic acid (epa; 20:5, n-3) greatly exceed those of arachidonic acid (aa; 20:4, n-6) in the tissue phospholipids of most fish species. despite this, it is 20:4, n-6-derived eicosanoids that are produced predominantly in fish cells. the development of an essential fatty acid (efa)-deficient fish cell line would greatly assist the study of this selectivity and so several fish cell lines were cultured in efa-deficient (efad) media. all n-3 and n-6 polyunsaturated fatty acids (p ...19958596777
insulin/igf-i binding ratio in skeletal and cardiac muscles of vertebrates: a phylogenetic approach.insulin and insulin-like growth factor (igf-i) receptor binding and tyrosine kinase activity were characterized in cardiac and skeletal muscles of several vertebrates. specific insulin binding per unit weight of skeletal muscle was clearly higher in pigeon and rat than in ectothermic vertebrates (32 +/- 5 and 25 +/- 2.7%/100 mg initial tissue in pigeon and rat, respectively, vs. 4.4 +/- 0.2%/100 mg in carp samples). insulin binding clearly predominated over igf-i binding in skeletal muscle of en ...19958594939
[light and electron microscopic studies on the sporogenesis of sphaerospora molnari in the gill lamellas of carp (cyprinus carpio)].the sporogenesis of sphaerospora molnari in the gill lamellas of the common carp was investigated by light- and electron microscopy. whereas pseudoplasmodia were not identified, the development from one secondary cell containing two tertiary cells to the mature spore was pursued. monosporic sporoblasts surrounded by an envelope cell were observed lying isolated in the host tissue. sporoblast generative cells differentiated into two capsulogenic cells, two mononucleate sporoplasms and two valvoge ...19958593138
regional distribution of vasoactive intestinal peptide immunoreactivity in the brain of salmon, trout and carp.the content of vasoactive intestinal peptide (vip) was determined by radioimmunoassay in different brain regions of three fish species, namely salmon, trout and carp. the vip extracted from salmon brain was shown by reverse-phase high-performance liquid chromatography to coelute with native porcine vip. highest vip levels were measured in the salmon brain with a maximum of 112 pg/mg protein in the hypothalamus, followed by the telencephalon, olfactory bulb and optic tectum. in contrast, in the t ...20068590917
the complete nucleotide sequence of the mitochondrial dna genome of the rainbow trout, oncorhynchus mykiss.the complete nucleotide sequence of the mitochondrial dna of the rainbow trout, onchorynchus mykiss, has been determined. the total length of the molecule is 16,660 bp. the rainbow trout mitochondrial dna has the same organization described in eutherian mammals, the clawed frog (xenopus laevis), and the two fish species, oriental stream loach (crossotoma lacustre) and carp (cyprinus carpio). alignment and comparison of the deduced amino acid sequences of the 13 proteins encoded by rainbow trout ...19958587139
detection of spring viremia of carp virus by hybridization with biotinylated dna probes. 19958581035
comparison of the polymerases (l genes) of spring viremia of carp virus and infectious hematopoietic necrosis virus.we have determined the partial nucleotide sequences of the polymerase genes of the fish rhabdoviruses, spring viremia of carp virus (svcv) and infectious hematopoietic necrosis virus (ihnv). at this point we have deduced the amino acid sequences and analysed the first 1,400 amino acids comprising two thirds of the polymerase genes of svcv and ihnv. we have compared sequence similarities of svcv and ihnv polymerases with other rhabdovirus and paramyxovirus polymerases. the svcv polymerase showed ...19958581012
eighteen years of vaccination against viral haemorrhagic septicaemia in france.viral haemorrhagic septicaemia (vhs) has been considered for many years to be a major cause of loss in the french trout industry. the high prevalence of vhs in certain geographic areas made a control strategy based on control policy unfeasible. this provided the impetus for immunoprophylaxis development that resulted in 3 successive types of vaccines: inactivated, live attenuated and recombinant vaccines. when delivered by intraperitoneal injection, the 2 propiolactone-inactivated vhs virus was ...19958581010
effect of potassium permanganate, cobalt chloride and vitamin b complex on the haematological parameters of cadmium treated common carp, cyprinus carpio.effect of potassium permanganate, cobalt chloride and vitamin b complex on the haematological parameters of common carp cyprinus carpio were markedly influenced by a treatment of 2.5 mg/l cd in the laboratory for 96 hours. hb% and esr values were increased but the rbc and pcv values were reduced. treatment of kmno4 (1 mg/l) or cocl2 (2 mg/l) induced a further reduction of rbc of the cd treated fish. but parameters like pcv, mcv and mch of cd treated fish were not affected by the treatment of kmn ...19958580524
investigations into the ubiquitous nature of high or low immune responsiveness after divergent selection for antibody production in common carp (cyprinus carpio l.).this paper reports on the selection of individual carp with a high or low antibody response, in combination with reproduction by gynogenesis, in order to develop well-characterised inbred carp lines consisting of practically unlimited numbers of carp with the same genotype. two homozygous progenies, previously characterised as having a high or low immune response to dinitrophenyl keyhole limpet haemocyanin (dnp-klh), were immunised with either a t-dependent (dnp-human serum albumin (dnp-hsa)) or ...19958578693
alkaline single cell gel (comet) assay and genotoxicity monitoring using bullheads and carp.monitoring genotoxicity of the environment using endemic organisms as sentinels requires the development of sensitive assays. toward this end, we explored the feasibility of applying the alkaline single cell gel (scg) or "comet" assay. this approach involves detection, under alkaline conditions, of cell dna fragments which, on electrophoresis, migrate from the nuclear core, resulting in a "comet with tail" formation. tail length has been correlated with level of genotoxicant exposure in a number ...19958575424
a mother and three daughters with congenital dislocation of the hip and a characteristic facial appearance: a new syndrome?we describe a mother and her three daughters who all have bilateral congenital dislocation of the hip. the mother has had no other medical problems and is on the 90th centile for height. her three daughters resemble each other strongly with facial characteristics which include hypertelorism, epicanthic folds, puffiness around the eyes, a flat mid-face and a carp-shaped mouth. all three daughters are on the 3rd centile for height, with their head circumference on a higher centile and all had an a ...19958574416
dermocystidium koi found in skin lesions in koi carp (cyprinus carpio). 19958571508
toxic effects, at three ph levels, of two reference molecules on common carp embryo.early life-stage survival, motility, and growth toxicity tests were carried out on common carp (cyprinus carpio l.) endotrophic embryo, with two reference molecules (captafol and dnoc), from fertilization to the end of the first two-thirds period of mortalities by starvation. thirteen days duration exposure was performed in daily renewed medium, at 24.5 degrees c, in standard synthetic water, at three phs (6.9, 7.8, 9.0), in the presence of 10 mm/liter nontoxic ph buffer. nominal concentrations ...19958565873
[the perceptive brightness space in the carp (carpio cyprinus l.)].brightness discrimination of black-white (in the range of 0.0082-0.214 w/m2; cie chromaticity coordinates x = 0.340, y = 0.354) and red (in the range of 0.0035-0.106 w/m2; cie chromaticity coordinates x = 0.641, y = 0.342) stimuli was studied during using choice conditioning paradigm in two carps. confusion matrices for black-white and red stimuli separately were constructed from probabilities of instrumental responses (catching of a bead) to conditioned and differential stimuli in sequential si ...20068560943
studies on the structure and function of the carp gonadotropin alpha subunit by site-directed mutagenesis.there are two genes encoding the alpha subunit of carp gonadotropin (alpha 1 and alpha 2 subunits). our previous data have demonstrated that both alpha 1 and alpha 2 subunits expressed in insect cells are able to associate with the beta subunit, but only the alpha 1/beta heterodimer displays biological activity. in the mature protein, there are only four amino-acid residues different between the alpha 1 and alpha 2 subunits. in this study we used site-directed mutagenesis and expressed different ...20068537173
somatostatin-, vasoactive intestinal peptide-, and granulin-like peptides isolated from intestinal extracts of goldfish, carassius auratus.three new peptides were originally isolated from intestinal extracts of goldfish. they were structurally related with somatostatin, vasoactive intestinal peptide (vip), and granulin (grn) and thus termed goldfish somatostatin (gss-28), gvip, and ggrn, respectively. the primary structures of these peptides were determined as: svessnhlpa 10rerkagcknf20ywkgftsc for the gss-28; hsdavftdny10sryrkqmaak20kylnsvla-nh2 for the gvip, and vihcdsstic10 pdgttcclsp20ygvwyccpfs30mgqccrdgih40ccrhgyhcds50tsthclr ...19958536941
evidence that the inhibitory effects of stress on reproduction in teleost fish are not mediated by the action of cortisol on ovarian steroidogenesis.ovarian follicles of goldfish, common carp, and the sparid pagrus auratus (new zealand snapper) were incubated in vitro to assess the effects of cortisol (f) on ovarian steroidogenesis. unstimulated goldfish follicles produced little testosterone (t) or 17 beta-estradiol (e2), whereas both carp and snapper follicles spontaneously produced e2 and to a lesser extent t. goldfish follicles produced increased amounts of e2 in response to treatment with human chorionic gonadotropin (hcg), whereas carp ...19958536936
[high molecular weight forms of aminoacyl-trna synthetases in animals. ii. high molecular weight aminoacyl-trna synthetase complexes in lower animals].high-molecular-weight aminoacyl-trna synthetase complexes have been found in hen, frog and carp liver cytosol as well as in cytosol from snails and paramecium. the complexes from vertebrates and snails contained 13-15 aminoacyl-trna synthetases, revealed mass of about 4,000 kda and sedimentation constant about 19 s. the complex from paramecium containing 11 aminoacyl-trna synthetases, revealed a mass of about 1,600 kda and sedimentation constant 15 s. the results of the present study support the ...19938534161
a new fluorochromasia method using a fluorescence microplate reader for assay of cytotoxic activity of carp leucocytes.a new fluorochromasia method using a fluorescence microplate reader has been established to assay spontaneous cytotoxic activity of carp leucocytes. this method is characterized by using propidium iodide (pi) for staining dead target cells and a fluorescence microplate reader for measurement of the fluorescence of pi. k562 as target cells were prepared in 96-well flat-bottom microplates, and carp leucocytes were added as effector cells. after 2.5 h incubation, pi was added to each well. after an ...19958533295
genetic variation in susceptibility to trypanoplasma borreli infection in common carp (cyprinus carpio l.).gynogenetic reproduction of homozygous females, or crossbreeding two homozygous animals, results in fish lines without genetic variation. hybrid crosses are expected to express a more stable development than homozygous lines, the latter may have an important value for gaining insight into genetic components of host resistance to parasite infection. we examined the antibody response of carp (cyprinus carpio l.) to infection with trypanoplasma borreli. outbred carp responded with a production of s ...19958533293
retraction of spinule-type neurites from carp retinal horizontal cell dendrites during dark adaptation involves the activation of ca2+/calmodulin-dependent protein kinase ii.the formation of spinules at the terminal dendrites of retinal horizontal cells with the onset of light and their subsequent retraction during darkness is a remarkable example of synaptic plasticity where sensory experience modifies reversibly, and on a time scale of minutes the ultrastructure of synaptic connectivity. the signals and the subsequent intracellular cascades underlying the prominent morphological alterations are only partially understood. we show here that lowering the external cal ...19958528466
the effect of dietary energy and protein concentration and feeding level on feed utilization and body composition of carp (cyprinus carpio l.).a three factorial designed feeding experiment with common carp (cyprinus carpio l.) was carried out in an intensive experimental rearing system. fish (initial body weight 200 g) were fed on two different levels of dietary energy (16 and 18 mj de/kg dm), two different levels of protein (320 and 420 g cp/kg dm) and also two different feeding intensities (100% and 75% of the maximum intake). the experiment was terminated when fish reached a mean body weight of 1300 g. growth, feed utilization and n ...19958526728
localization, trafficking, and temperature-dependence of the aequorea green fluorescent protein in cultured vertebrate cells.the localization, trafficking, and fluorescence of aequorea green fluorescent protein (gfp) in cultured vertebrate cells transiently transfected with gfp cdna were studied. fluorescence of gfp in uv light was found to be strongest when cells were incubated at 30 degrees c but was barely visible at an incubation temperature of 37 degrees c. cos-1 cells, primary chicken embryonic retina cells, and carp epithelial cells were fluorescently labeled under these conditions. gfp was distributed uniforml ...19958524871
uptake and tissue distribution of dietary and aqueous cadmium by carp (cyprinus carpio).the aim of this study was to assess whether contaminated prey can be a major source of cd for fish. for this purpose, the uptake and tissue distribution of dietary and aqueous cadmium by the carp (cyprinus carpio) was studied. the fish were exposed to either cd-contaminated food or cd-contaminated water for 4 weeks in laboratory experiments. when exposed to contaminated food, the fish were kept in clean water and fed contaminated chironomid larvae, equilibrated with 99 micrograms cd. liter-1. du ...19958521785
an aquatic toxicological evaluation of fenthion in the context of finch control in south africa.queletox, containing fenthion as active ingredient, is the avicide formulation used in south africa to control red-billed finches (quelea quelea). control measures involve night spraying of roosting areas with a light aircraft. since roosting areas often include reedbeds along riversides and on islands, proper control is difficult without exposing the aquatic environment to some risk of contamination. this study tested the acute effects of fenthion, in association with the queletox formulation, ...19958521783
advanced rus and normal carpal bone age in childhood obesity.skeletal maturation was evaluated in 60 prepubertal children aged 7.7 +/- 0.27 years (range 2.7-12.6). the radius, ulna and short bones (rus) and the carpal (carp) bone age were assessed. height corresponded to 1 +/- 0.1 z scores.19958520642
[digestion, growth and body composition of carp (cyprinus carpio l.) after feeding different starch varieties].maize starch (treatment i), pregelatinized maize starch (ii), wheat starch (iii), pregelatinized wheat starch (iv), potato starch (v) or manioc meal (vi) were mixed in portions of 41% (i-v) or 46.7% (vi) to a basal diet. each experimental diet was fed to 33 carp (cyprinus carpio l.) (three aquaria with 11 carp each) in a daily amount of 1.8-2.0% of live weight. the experiment lasted 118 days and the carp had a mean live weight of 146 g at the beginning. the diets i-iv had a similar, high energy ...19938517771
gaba- and glycine-immunoreactive neurons in the spinal cord of the carp, cyprinus carpio.gamma-aminobutyric acid (gaba) and glycine are the two main inhibitory transmitter amino acids in the central nervous system of vertebrates. the distribution of cells containing gaba and glycine in the carp spinal cord was examined by using specific antisera raised against the two amino acids conjugated to bovine serum albumin. the immunoreaction on serial paraffin sections was visualized by a streptavidin-biotin method. both antisera gave highly specific labelings of cells. at least three types ...19938514921
efficacy of fumagillin against thelohanellus kitauei infection of israel carp, cyprinus carpio nudus.the potential of fumagillin dicyclohexylamine salt to treat and prevent intestinal giant-cystic disease in israel carp, cyprinus carpio nudus, was monitored in field experimental studies. in experiment 1 (therapeutic), most fish were already naturally infected with more advanced stage of thelohanellus kitauei. fumagillin was administered to fish (mean body weight of 830 g) for a period of one month at a dose of 10.62 mg in the first group and 5.3 mg in the second group per fish per day. in exper ...19938512900
determination of ichthiomycin concentration by biological test with cyprinus carpio l.we have found out that one-year-old carps are extremely sensitive to ichthiomycin in concentrations between 25-125 micrograms/dm3. this fact permitted the use of biological methods for determination of ichthiomycin concentrations in cultural medium of streptomyces levoris 1107 or crude preparations of the antibiotic.19938511994
intestinal, segmented, filamentous bacteria in a wide range of vertebrate species.segmented, filamentous bacteria (sfbs) form a group of bacteria with similar morphology and are identified on the basis of their morphology only. the relationships of these organisms are unclear as the application of formal taxonomic criteria is impossible currently due to the lack of an in vitro technique to culture sfbs. the intestine of laboratory animals such as mice, rats, chickens, dogs, cats and pigs is known to harbour sfbs. to see whether this extends to other animal species, intestines ...19938501895
risk factors for kidney cancer in new south wales. iv. occupation.in a population based case-control study of kidney cancer in new south wales, data from structured interviews with 489 cases of renal cell cancer (rcc), 147 cases of renal pelvic cancer (carp), and 523 controls from the electoral roles were obtained about employment in certain industries or occupations, and exposure to particular chemicals chosen because of suspected associations with kidney cancer. a low level of education increased the risk for carp but not rcc. after adjustment for known risk ...19938494775
characterization of catch-relaxing peptide (carp) immunoreactive neurons in the helix nervous system.both small and large diameter of carp immunoreactive neurons could be observed in the different ganglia of cns of helix but not in the foot musculature. the immunoreactivity is the strongest in the varicose segments of immunoreactive fibers. the present findings suggest a transmitter or modulatory role of carp in both central and peripheral regulatory processes.19938493855
presence of growth hormone receptors in carp liver and intestine.membranes were prepared from tissues of the carp ctenopharynogodon idellus including liver, kidney, intestine, adipose tissue, ovary, gill, heart, muscle and spleen. the carp liver and intestine membranes bound 125i-labeled bovine growth hormone and the binding could be displaced by cold bovine growth hormone. no changes in the ability to bind 125i-labeled bovine growth hormone occurred after treatment of the carp liver membrane with dnase, rnase, alpha-amylase and beta-glucosidase, suggesting t ...19938490577
[the population level and distribution of ectoparasites in carp].the abundance and the distribution of dactylogyrus extensus and ichthyophthirius multifiliis on gills of two-years old carps have been investigated. it has been established that at least there are five groups, which differs from each other on the asymmetry of infection.19938488057
isolation and primary structure of the three major forms of granulin-like peptides from hematopoietic tissues of a teleost fish (cyprinus carpio).granulins are cysteine-rich polypeptides purified from human and rat hematopoietic cells and structurally related to the epithelin family of growth modulatory factors. a prototypic form of granulin was isolated from the hematopoietic organs of teleost fish (belcourt, d., and bennett, h.p.j. (1988) j. cell biol. 107, 629 (abstr.)). this study reports the structure of three granulins purified from the spleen and head kidney of the carp (cyprinus carpio). ion-spray mass spectrometric analysis of gr ...19938486624
tempo and mode of mitochondrial dna evolution in vertebrates at the amino acid sequence level: rapid evolution in warm-blooded vertebrates.by using complete sequence data of mitochondrial dnas, three markov models (dayhoff, proportional, and poisson models) for amino acid substitutions during evolution were applied in maximum likelihood analyses of mitochondrially encoded proteins to estimate a phylogenetic tree depicting human, cow, whale, and murids (mouse and rat), with chicken, frog, and carp as outgroups. a cow/whale clade was confirmed with a more than 99.8% confidence level by any of the three models, but the branching order ...19938483165
new beta chain-encoding mhc class ii genes in the carp. 19938482577
the deduced amino acid sequence of human carbonic anhydrase-related protein (carp) is 98% identical to the mouse homologue.a recently reported mrna, encoding 'carbonic anhydrase-related polypeptide' (carp) from the purkinje cells of mouse cerebellum, was shown to have a 30-40% deduced amino acid sequence identity with the carbonic anhydrases (ca) of mammals. in order to compare the mouse and human carp sequences, we used the polymerase chain reaction (pcr) to amplify human carp sequences from several cdna libraries (salivary gland, testis and placenta). the sequence has an 89.3% sequence identity with mouse carp at ...19938482548
pharmacokinetics of sulphadiazine and trimethoprim in carp (cyprinus carpio l.) acclimated at two different temperatures. 19938478994
an invertebrate calcium-binding protein of the calbindin subfamily: protein structure, genomic organization, and expression pattern of the calbindin-32 gene of drosophila.antisera against vertebrate calcium-binding proteins cross-react with drosophila nervous and muscle tissue. we have used an antiserum against carp parvalbumin to isolate from a drosophila head cdna library immunopositive expression clones. tissue in situ hybridization identified a clone that labeled specific neurons and muscles similar to the parvalbumin-like immunohistochemical staining pattern. five independent cdnas derive from an mrna whose open reading frame codes for a 310 amino acid polyp ...19938478695
[the distribution of lernaea cyprinacea (copepoda: lernaeidae) in a crucian carp population].differences in the infection with copepods and the character of distribution of occurrence frequencies of lernaea in crucian carp depending on the body length and sex of the latter are shown. the lowest infection rate with copepods was recorded for fishes with body length 8-11 cm as compared to specimens with body length 12-18 and 18.5-34 cm. the two latter groups did not differ from each other in this index. in males and females whose body length is 8-11 cm as well as in females with body lengt ...20078474768
variable sensitivity of cyprinus carpio eggs, larvae, and fry to pesticides. 19938467147
characterization of beta 2-microglobulin transcripts from two teleost species.using degenerate primers based on published beta 2-microglobulin sequences we were able to obtain an expected 111 base pairs (bp) polymerase chain reaction (pcr) fragment from tilapia genomic dna. the sequence of this fragment showed a high degree of similarity to mouse beta 2-microglobulin at the protein level. we used these primers in an "anchored pcr" to obtain a 213 bp pcr fragment from a carp cdna library. this was then used to clone a full-length beta 2-microglobulin cdna from carp. the ca ...19938462991
distribution of non-class-iii beta-tubulin isoforms in neuronal and non-neuronal cells.beta-tubulin isoforms in brain tissues and in cell lines were analyzed by high-resolution isoelectric focusing in combination with monoclonal antibodies. post-translational modifications of brain non-class-iii beta-tubulin isoforms were found in phylogenetically distant species ranging from pig to carp. less extensive modifications were also observed in neuro-2a, hela and 3t3 cells, where most acidic isoforms were glutamylated, while the basic, most abundant isoforms were not. the data suggest p ...19938462682
filament-forming domain of carp dorsal myosin rod.substructure of the myosin rod and its correlation to the filament formation were investigated by using fish myosin rod. it was found that fish rod contains a unique chymotrypsin susceptible site, 40 kda from the cooh-terminus or 20 kda downstream from the subfragment-2/light meromyosin junction (s-2/lmm junction). cleavage at this new site produced subfragment-2 possessing 95 kda subunit (95k s-2) and light meromyosin possessing 40 kda subunit (40k lmm). the latter is the shortest unit ever rep ...19938454573
plasmodium falciparum: the immune response in rabbits to the clustered asparagine-rich protein (carp) after immunization in freund's adjuvant or immunostimulating complexes (iscoms).the plasmodium falciparum clustered asparagine-rich protein (carp) is a merozoite-associated antigen which contains approximately 30% asparagine. analysis of the dna sequences located 5' of the cloned 1.4-kb carp gene in the p. falciparum genome suggests that this gene fragment may encode the complete carp and that the gene product is a protein of m(r) 50,000. to analyze the immunogenicity of carp, the gene was expressed as a fusion protein with staphylococcal protein a (spa-carp). immunization ...19938454022
the gut-associated lymphoid tissue (galt) of carp (cyprinus carpio l.): an immunocytochemical analysis.the galt of carp was studied with monoclonal antibodies reacting with carp ig or carp leukocytes, using (dual) immunofluorescence or immunogold staining on cryosections, cytocentrifuge slides, and cell suspensions of the intestine. the intestinal epithelium contained many ig-negative lymphoid cells and, in the hindgut, also many large ig-positive macrophages, which appeared to bind ig. the lamina propria contained numerous ig-positive lymphoid cells next to ig-negative lymphoid cells and granulo ...20068449251
brain sensitivity to anoxia in fish as reflected by changes in extracellular k+ activity.most vertebrates have a very limited tolerance to anoxia. the only exceptions to this rule are found among ectothermic species, notably crucian carp (carassius carassius) and freshwater turtles. it has been assumed that the brains of these species are less sensitive to anoxia than ectothermic brains in general. however, it has not been possible to exclude that anoxia-intolerant species like rainbow trout (oncorhynchus mykiss) die from heart failure rather than brain failure during anoxia, and th ...19938447481
spinule-type neurite outgrowth from horizontal cells during light adaptation in the carp retina: an actin-dependent process.dendrites of horizontal cells in the carp retina which invaginate the cone pedicles form numerous spinules during light adaptation. we have analyzed the contribution of cytoskeletal elements to this process. isolated horizontal cells and frozen sections were screened with phalloidin for the existence of f-actin. f-actin was present in all types of horizontal cells and particularly enriched in the distal parts of the dendrites. electron microscopical analysis demonstrated that interruption of the ...19938445409
cloning and sequence analysis of cdnas encoding the mhc class ii beta chain in atlantic salmon (salmo salar).atlantic salmon (salmo salar) cdnas encoding the major histocompatibility complex (mhc-sasa) class ii beta chain were isolated from a leucocyte library by a polymerase chain reaction (pcr) approach. three different cdnas (c144, c22, and c157) encoding the entire mature beta chain have been analyzed. clone c144 differs from clone c157 in 12.6% of the nucleotides in the beta 1-encoding region. the corresponding differences between clones c144 and c22, and clones c22 and c157, are 10.3% and 5.2%, r ...19938436418
recombinant carp (cyprinus carpio) growth hormone: expression, purification, and determination of biological activity in vitro and in vivo.carp growth hormone (cgh) cdna (koren et al., 1989) was cloned under the control of lambda-phage plol promoter and lambda cll ribosomal binding site into pbr322 plasmid to enable its expression in escherichia coli a1645 that produces constitutively the thermolabile lambda repressor c1857. temperature shift to 42 degrees abolished the repression, resulting in a high level of cgh expression. the bacterially expressed cgh protein, contained within the refractile body pellet, was solubilized in 4.5 ...19938428649
acute toxicity of selected organophosphorus pesticides to cyprinus carpio and barilius vagra.the acute toxicity of two organophosphorus (op) pesticides, diazinon and malathion to cyprinus carpio and barilius vagra was determined. mortalities and lc50-96 hr values for b. vagra and c. carpio were variable. both juvenile common carp and b. vagra were extremely sensitive to diazinon. adult b. vagra were more sensitive to malathion than were juvenile common carp.19938426062
hypo-osmotic shock induces an osmolality-dependent permeabilization and structural changes in the membrane of carp sperm.we carried out spectrofluorimetric and flow cytometric measurements to investigate the effect of hypo-osmotic shock on cell membranes of common carp sperm. the time course of the permeability of the sperm cell membrane, as monitored by dna-related propidium iodide (pi) fluorescence, was followed for 30 min after dilution of semen in hypo-osmotic environments of different ionic strengths. spectrofluorimetric measurements indicated a continuous increase in the total pi emission intensity of a sper ...19938419464
efficiency of introns from various origins in fish cells.several vectors containing (1) regulatory regions from rous sarcoma virus (rsv), human cytomegalovirus (cmv), and herpes simplex thymidine kinase (tk); (2) introns from early or late sv40 genes and from trout growth hormone gene (tgh); (3) chloramphenicol acetyltransferase gene (cat); and (4) transcription terminators from sv40 were transfected into carp epc cells, salmon chse cells, tilapia to2 cells, quail qt6 cells, and hamster cho cells. cat activity was measured in extracts from several cel ...19938395951
the phylogenetic position of the zebrafish (danio rerio), a model system in developmental biology: an invitation to the comparative method.the zebrafish, danio (brachydanio) rerio, has become one of the most widely studied model systems in developmental biology. we present a dna-based phylogeny of zebrafish and other species of the genus danio, and the genera rasbora, puntius and cyprinus. homologous regions of the large (16s) mitochondrial ribosomal rna gene were amplified by the polymerase chain reaction and directly sequenced. the phylogeny revealed: (i) the zebrafish, danio (brachydanio) rerio, is identical in its 16s sequence ...19938394584
toxic effects of industrial effluents on hatchability and viability of cyprinus carpio eggs. 19938387839
cloning and characteristics of fish glial fibrillary acidic protein: implications for optic nerve regeneration.mammalian central nervous system neurons do not regenerate after axonal injury, unlike their counterparts in fish and amphibians. after axonal injury, glial cells in mammals do not support regrowth of axons, while in fish they support the regeneration process. controversy exists as to whether or not the intact fish optic nerve expresses glial fibrillary acidic protein, a well-known marker for mature astrocytes, and thus whether its astrocytes differ in this respect from those of the brain and sp ...19938376626
differences in mucus and serum immunoglobulin of carp (cyprinus carpio l.).electrophoretic analysis did not reveal clear differences between skin mucus and serum immunoglobulin (ig) of carp. the majority of both igs were tetrameric (+/- 760 kda) and composed of 25 kda light (l) chains and 70 kda heavy (h) chains, but dimeric and monomeric forms were found as well. monoclonal antibody (mab) wci 12 produced from serum ig appeared to react with the h chain of both molecules. after immunisation of mice with purified mucus ig, mabs could be selected that were reactive with ...20038375566
organochlorine pesticides and polychlorinated biphenyl congeners in commercial fish from the great lakes.fillets from 11 species of commercial fish from the great lakes were analyzed for residues of 39 polychlorinated biphenyl (pcb) congeners and 24 other organochlorine compounds. eel and trout contained the highest amount of pcb (753 and 633 ppb wet weight, respectively) and other organochlorines (607 and 1404 ppb, respectively); perch and carp contained the lowest residues. the penta- and hexachlorinated pcbs were the major congeners in all species except whitefish, in which the tetrachlorinated ...20138374320
vimentin persists in the mature glia of fish brain.adult carp brains were processed for immunohistochemical staining against vimentin and glial fibrillary acidic protein. the shape, density and distribution of immunopositive structures were found to be very similar with both types of antibody. thus, it appears that vimentin, the cytoskeletal protein typical for immature glia in the early phase of ontogenesis in mammals can also be demonstrated in the adult glia of the phylogenetically older fish brain.19938369799
a pulsed photolysis procedure for determining oxygen equilibrium parameters of low-affinity noncooperative hemoglobins.it is often very difficult to obtain precise values for oxygen equilibrium constants for low-affinity noncooperative hemoglobins owing to the ease with which they are oxidized and denatured with even the most gentle stirring or agitation. we have developed a procedure that eliminates this problem by maintaining the hemoglobin as hbco except during the brief time it is present as hb, and hbo2 forms following photolysis. oxygen is continuously removed by an enzyme system. the solution is photolyze ...19938367536
expression and inheritance of rsvltr-rtgh1 complementary dna in the transgenic common carp, cyprinus carpio.transgenic common carp, cyprinus carpio, possessing the long terminal repeat (ltr) sequence of avian rous sarcoma virus (rsv) fused to the rainbow trout (rt) growth hormone (gh1) complementary dna (cdna) were produced by microinjection. initial studies showed that the transgenic common carp transmitted the foreign dna to a significant fraction of their progeny in three of four crosses of transgenic males with control females. these progeny grew 20 to 40% faster than their nontransgenic full sibl ...19938364692
regulation of expression of transgenes in developing fish.the transcriptional regulatory elements of the beta-actin gene of carp (cyprinus carpio) have been examined in zebrafish and goldfish harbouring transgenes. the high sequence conservation of the putative regulatory elements in the beta-actin genes of animals suggested that their function would be conserved, so that transgenic constructs with the same transcriptional control elements would promote similar levels of transgene expression in different species of transgenic animals. to test this assu ...19938353534
cyclic nucleotide-activated channels in carp olfactory receptor cells.when applied from the cytoplasmic side, cyclic 3',5'-adenosine and guanosine monophosphates reversibly increased the ion permeability of inside-out patches of carp olfactory neuron plasma membrane. the camp (cgmp)-induced permeability via camp (cgmp) concentration was fitted by hill's equation with the exponents of 1.07 +/- 0.15 (1.12 +/- 0.05) and ec50 = 1.3 +/- 0.6 microm (0.9 +/- 0.3 microm). substitution of nacl in the bathing solution by chlorides of other alkali metals resulted in a slight ...19938334139
effects of inoculum route and dose on the immune response of common carp, cyprinus carpio to the blood parasite, trypanoplasma borreli.carp were inoculated intramuscularly (im) or intraperitoneally (ip) with different doses of live trypanoplasma borreli. prepatent period was shortened by using the im route and by increasing the dose. haematocrit was reduced following im inoculation with 9.5 x 10(5) parasites. most fish recovered between 8 and 13 weeks post-inoculation (wpi). neither parasitaemia nor anaemia was detected in any recovered fish following homologous challenge. an enzyme-linked immunosorbent assay was developed to q ...19938333145
expansion of genes that encode mhc class i molecules in cyprinid fishes.we have analyzed the genomic genes and the mrna that encode mhc class i molecules in cyprinid fishes, namely, the ginbuna crucian carp (carassius auratus langsdorfii) and the carp (cyprinus carpio). the presence of several distinct class i genes was revealed in the genome of cyprinid fishes, and distinct class i genes that are expressed in carp cells were identified. the sequences of the alpha 3 domain of these class i genes are relatively well conserved, whereas the sequences of the respective ...19938326125
a coccidian (apicomplexa: eimeriidae) with extracytoplasmally located stages in the kidney tubules of golden carp (carassius auratus gibelio l.) (cyprinidae).numerous coccidian stages were found in the kidney tubules of the golden carp (carassius auratus gibelio). the merogonial and gamogonial stages were localized extracytoplasmally in the microvillous region of the epithelial cells. the host-parasite interface consisted of i) a large area where the parasite was separated from the host cytoplasm by the parasitophorous vacuole membrane only, and ii) a zone of multiple fusions of the host cell membrane investing the parasite to the neighbouring microv ...19938325562
purification and properties of an indian major carp (cirrhinus mrigala, ham.) pituitary thyrotropin.thyroid stimulating hormone (tsh) was isolated and purified from the pituitaries of indian major carp, cirrhinus mrigala, with the help of a sensitive in vitro bioassay system, based on incubating isolated thyroid follicles from murrel, channa gachua. in this assay, addition of test material containing tsh increased thyroxine (t4) release into the medium which was then measured by radioimmunoassay (ria). tsh activity was eluted as one major peak on sephadex g-100 gel filtration (peak i), which a ...19938319876
the occurrence of sphaerospora renicola k-stages in the choroidal rete mirabile of the common carp.it is known from earlier studies that the so-called k-protozoa, the late presporogonic stages of sphaerospora renicola, a parasite causing renal sphaerosporosis in common carp (cyprinus carpio), accumulate in the swimbladder wall and give rise to the disease called myxosporean swimbladder inflammation. the present study indicates that in the case of intensive infection these parasites also occur in the rete mirabile of the choroid of the eye, causing haemorrhages and necrosis. the results sugges ...19938314174
molecular cloning and sequencing of a carp cdna encoding mitogen-activated protein kinase kinase.we have cloned a full-length cdna encoding the mitogen-activated protein kinase kinase (mkk) from a carp liver cdna library. the cdna contains 1970 bp with a single open reading frame encoding a 397 amino acid protein. by comparing with known mkk sequences from other species, carp mkk is 78%, 80%, 76% and 58% identical to rat mkk1, rat mkk2, xenopus mkk and drosophila mkk.19948312367
'broken backs' in koi carp (cyprinus carpio) following lightning strike. 19938296436
localized leucocyte response to ichthyophthirius multifiliis establishment in immune carp cyprinus carpio l.o-group carp (cyprinus carpio) which had been immunized against ichthyophthirius multifiliis by controlled infections were challenged by topical application of theronts to the caudal fin. the parasites which established were examined ultrastructurally, and host leucocyte responses were compared with those observed in primary infections. in the primary exposure group eosinophils and (to a lesser extent) basophils were the predominant cells infiltrating infection sites. in contrast, parasite devel ...19938291210
effects of prolonged darkness on light responsiveness and spectral sensitivity of cone horizontal cells in carp retina in vivo.light responses of cone- and rod-driven horizontal cells were recorded intracellularly from opened eyes of intact, immobilized carp and the effects of prolonged darkness on these cells were examined. when the retina was left in the dark, responses of cone horizontal cells to a moderate test flash presented once every 10 min gradually and steadily decreased in size (dark suppression effect). following a repetitive presentation of the test flash, the light responsiveness of the cells was enhanced. ...19948283240
antibody dependent haemolysin, complement and opsonin in sera of a major carp, cirrhina mrigala and catfish, clarias batrachus and heteropneustes fossilis.the present communication is a continuation of our earlier study on the natural serum haemagglutinin/lectins of cirrhina mrigala, clarias batrachus and heteropneustes fossilis. sera of cirrhina mrigala, belonging to the major carp family, could not only agglutinate heterologous rabbit erythrocytes, but also lyse them spontaneously. this lysis of rabbit rbc by cirrhina mrigala sera was calcium ion dependent and heat sensitive, indicating thereby that the haemolysis was mediated by the fish serum ...19938281746
influence of seasonal adaptation on the distribution of cytochemically detectable endogenous calcium in synaptic terminals of the optic tectum of crucian carp (carassius carassius l.).crucian carps were seasonally adapted to high (23 degrees c) or low (4 degrees c) temperatures and synaptic regions in the optic tectum were analyzed by means of energy-filtering transmission electron microscopy (eftem). the amount of cytochemically detectable endogenous calcium in synaptic clefts was quantified by means of image analysis and the amount of glycogen granules in synaptic regions was assessed. in cold-adapted fish, glycogen is increased, especially in glial cells, while the calcium ...19938270788
the branching of insulin-like growth factor 1 and insulin: an immunohistochemical analysis during phylogeny.the co-existence of insulin-like growth factor 1 (igf-1) with the classical islet hormones insulin (ins), glucagon (gluc), somatostatin (som) and pancreatic polypeptide (pp) in the endocrine pancreas of representative species of cyclostomes (myxine glutinosa), cartilaginous fish (raja clavata, squalus acanthias) and bony fish (cottus scorpius, carassius auratus, cyprinus carpio, anguilla anguilla) was studied by the use of monoclonal and polyclonal antisera and the double immunofluorescence tech ...19938265818
cytoarchitecture, fiber connections, and ultrastructure of the nucleus pretectalis superficialis pars magnocellularis (psm) in carp.the cytoarchitecture, fiber connections, and ultrastructure of the nucleus pretectalis superficialis pars magnocellularis (psm) were studied in cypriniform teleosts (cyprinus carpio). the psm is an oval nucleus in the pretectum. medium-sized cells and synaptic glomeruli are the main components of the nucleus. a lesser number of small cells are also present. most of the medium-sized cells form one or two cell layers on the periphery of the nucleus, and some cells are scattered among synaptic glom ...19938263230
in vitro phosphorylation in isolated horizontal cells of the fish retina: effects of the state of light adaptation.horizontal cells, which are second-order neurons of the vertebrate retina, exhibit synaptic plasticity governed by light and dark adaptation. we have investigated the alterations in the protein phosphorylation patterns of isolated carp (cyprinus carpio) horizontal cells in relation to their state of light adaptation by using an in vitro phosphorylation assay and compared the resulting data with protein synthesis patterns of the whole retina. phosphoproteins and [35s]methionine-labelled proteins ...19938261133
effect of lethal and sublethal concentrations of cadmium on energetics in the gills of fry and fingerlings of cyprinus carpio. 19938257822
the expression of rat gap-43 cdna in transgenic carp.to understand more about growth-associated protein-43 (gap-43), we produced transgenic carp by introduction of a rat gap-43 cdna linked to the rous sarcoma virus-long terminal repeat into fertilized eggs. of 180 eggs microinjected with exogenous gene, 59 embryos hatched and 4 fish were found to contain the exogenous gene sequences in the genomic dna. from a mature female transgenic carp, parthenogenetically, 126 progeny were derived and 52 of them survived for more than 90 days. the exogenous ge ...19938257558
neuropeptide y in the carp torus semicircularis: an immunocytochemical study.the presence of neuropeptide y-like immunoreactivity in the torus semicircularis of the teleost fish cyprinus carpio was studied using an indirect immunoperoxidase technique. in general, both the nuclei lateralis and centralis of the torus semicircularis showed a high density of immunoreactive fibers from rostral to caudal levels. in particular, in the nucleus lateralis a low density of immunoreactive fibers was only found in the subependymal layer, whereas this was high in the small cell, fibri ...19938250673
multiphasic allometry.a multiphasic linear model is presented that describes the allometric relation between body components and permits a smooth transition from one linear segment to the next. three applications of a diphasic linear model to allometric growth problems were used to illustrate the model. the data sets were selected because a breakpoint exists in the allometric relation. relations between growth of the alimentary tract and of empty body weight in rabbits, growth of length and of body weight in larval-c ...19938244622
[localization and activity of several enzymes in tissues of bothriocephalus acheilognathi and khawia sinensis tapeworms parasitizing carp].in this study, an attempt has been made to compare the most common species of tapeworms in carp: non-segmentary khawia sinensis and segmentary bothriocephalus acheilognathi on the basis of histoenzymatic examination of 8 enzymes, i.e. alkaline phosphatase, acid phosphatase, adenosine triphosphatase, nonspecific esterase alfa, succinic dehydrogenase, lactate dehydrogenase, leucine aminopeptidase and gamma-glutamylo transferase.19938237009
estimation of the genetic variation in complement activity of common carp (cyprinus carpio l.).the complement status of hybrid, laboratory raised carp was determined by an in vitro approach of the alternate complement activity (ach50) and total haemolytic activity (ch50), and by measurement of serum c3 levels. the lysis of target sheep red blood cells (rbc) in the haemolytic assay for ch50 activity depended, amongst others, on the haemolysin concentration in the assay. rocket electrophoresis showed a mean serum c3 concentration of 0.95 mg ml-1. the variation for both ach50 and ch50 haemol ...19938236805
dna adduct detection: some applications in monitoring exposure to environmental genotoxic chemicals.in the assessment of genotoxic risk factors in the environment, the measurement of dna adducts in aquatic organisms and in plants may have considerable implications. using 32p-postlabelling, we have detected dna adducts in the liver of carp (chondrostoma nasus) from the river rhône (france), both downstream and upstream from a polychlorinated biphenyl incineration plant. some of the dna adducts were specific to downstream fish, suggesting a differential pattern of exposure. we have also detected ...19938225509
biomonitoring of aquatic systems.the 32p-postlabelling analysis provides a sensitive means for detecting pollution-related dna adducts in aquatic organisms exposed to environmental carcinogens. however, the following factors need to be taken into consideration during the data interpretation: (1) species-specific, naturally occurring dna modifications (or i-compounds) are found in aquatic organisms at levels which are highly season-dependent; and (2) many aquatic organisms, particularly lower invertebrates, cannot form dna adduc ...19938225508
studies on the hypocalcemic actions of salmon calcitonin and ultimobranchial gland extracts in the freshwater teleost cyprinus carpio.a single injection of synthetic salmon calcitonin (sct) or homologous ultimobranchial gland (ubg) extract caused a significant reduction in total and ultrafiltrable plasma ca2+ in a freshwater teleost, cyprinus carpio. a time-bound analysis on the effect of sct or ubg extract caused a short-duration hypocalcemia and the response was more pronounced in fish kept in low-ca2+ water. sexually immature fish showed a greater response than did mature fish. in all the studies the protein-bound ca2+ frac ...19938224753
the effect of 75% glycerol on the oxygen binding properties of carp hemoglobin.at ph 6 in the presence of inositol hexaphosphate, ihp, conditions where ligand-saturated carp hemoglobin is already in the low affinity t state, the addition of glycerol has little effect on affinity and ligand binding remains noncooperative. at all other ph values examined, with and without ihp, the effect of glycerol is to lower oxygen affinity possibly by shifting the equilibrium between the t state and the high affinity r state in the direction of the t state. although glycerol does not app ...19938216252
comparative studies on fish and bird insulin receptors.the comparative studies were performed on insulin receptors of erythrocytes and liver plasma membranes in fish (tench and carp) and bird (duck). the scatchard plots indicated the presence of two pools of binding sites both in fish and duck. these pools show inter-species differences in binding ability and the number of receptors. specific binding of insulin and the binding affinity are higher in duck than in fish.19938212960
[cystic changes of the skin caused by dermocystidium koi in koi carp].skin infection in koi carp by dermocystidium koi is described. symptoms of disease and the parasite are illustrated.19948209371
response of serum calcium to administration of 1,25-dihydroxyvitamin d3 in the freshwater carp cyprinus carpio maintained either in artificial freshwater, calcium-rich freshwater or calcium-deficient freshwater.freshwater carps (cyprinus carpio) were injected daily intraperitoneally either with vehicle (0.1 ml 95% ethanol/100 gm body wt), or 1,25(oh)2d3 (5 iu/100 gm body wt) and maintained either in artificial-freshwater, calcium-rich freshwater or calcium-deficient freshwater for 15 days. the specimens were sacrificed on day 1, 3, 5, 10 and 15 after initiation of the experiment and serum calcium levels were analysed. (i) artificial freshwater: serum calcium level of 1,25(oh)2d3 treated specimens incre ...19938197882
a case-control study of oral cancer in beijing, people's republic of china. associations with nutrient intakes, foods and food groups.a case-control study of oral cancer was conducted in beijing, people's republic of china to examine the association between dietary nutrient intake and risk of oral cancer, both in terms of estimated intake of nutrients and micro-nutrients, and in terms of specific foods and food groups. the study was hospital-based and controls were hospital in-patients matched for age and sex with the cases. the response rate for cases and controls was 100% and 404 case/control pairs were interviewed. the resu ...19938180577
isolation and stability of partially oxidized intermediates of carp hemoglobin: kinetics of co binding to the mono- and triferric species.the monoliganded and triliganded forms of the asymmetric valency hybrids of carp hemoglobin were isolated using high-performance liquid chromatography. these partially oxidized hybrids were shown to be sufficiently stable to permit the measurement of the kinetics of co binding. the effects of protons and inositol hexaphosphate on the rates of these reactions were examined. the kinetics of co recombination with these partially oxidized derivatives were compared to the kinetics of co binding to th ...19948180217
seasonal variations in gonadotropin responsiveness, self-priming, and desensitization to gnrh peptides in the common carp pituitary in vitro.seasonal variations of the gth release response to salmon gnrh (sgnrh) and [d-arg6,pro9net]-sgnrh (sgnrh-a) were investigated in female common carp at different stages of the reproductive cycle using perifused pituitary fragments. the responsiveness to sgnrh and sgnrh-a varied seasonally in common carp pituitaries in vitro, with the greatest gth release response in pituitaries from sexually mature (preovulatory) fish compared to pituitaries from sexually regressed fish. the magnitude of this sea ...19948174932
the complete nucleotide sequence and gene organization of carp (cyprinus carpio) mitochondrial genome.the complete sequence of the carp mitochondrial genome of 16,575 base pairs has been determined. the carp mitochondrial genome encodes the same set of genes (13 proteins, 2 rrnas, and 22 trnas) as do other vertebrate mitochondrial dnas. comparison of this teleostean mitochondrial genome with those of other vertebrates reveals a similar gene order and compact genomic organization. the codon usage of proteins of carp mitochondrial genome is similar to that of other vertebrates. the phylogenetic re ...19948169959
in vivo inhibition of ache and atpase activities in the tissues of freshwater fish, cyprinus carpio exposed to technical grade cypermethrin. 19948167458
cloning and sequencing the genes encoding goldfish and carp ependymin.ependymins (epns) are brain glycoproteins thought to function in optic nerve regeneration and long-term memory consolidation. to date, epn genes have been characterized in two orders of teleost fish. in this study, polymerase chain reactions (pcr) were used to amplify the complete 1.6-kb epn genes, gf-i and cc-i, from genomic dna of cypriniformes, goldfish and carp, respectively. amplified bands were cloned and sequenced. each gene consists of six exons and five introns. the exon portion of gf-i ...19948163195
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