Publications

[immunotherapy today. ii. immunostimulating therapy].		1978	93770
[changes in the physiologic properties of a chemostat culture of propionibacterium shermanii during growth limitation by a deficiency of sodium lactate].	a chemostat curve has been constructed for changes in the propionibacterium shermanii population density with an increase in the flow rate of the medium from 0.04 to 0.55 h-1 upon limitation of the growth with sodium lactate deficiency. the curve differs from the classic chemostat curve described with the monod equation, thus indicating changes in metabolism at different growth rates. fermentation of lactate to acetic and propionic acids by the culture is most complete at low flow rates of the m ...	1979	93687
quantitation of skin bacteria: lethality of the wash solution used to remove bacteria.	a widely used technique for the quantitative removal of bacteria from the skin uses a detergent, triton x-100 (p, t-octylphenoxynonaethoxyethanol), to remove and suspend the bacteria. we determined the half-life for the survival of five common skin bacteria suspended in the solution. the shortest-lived was streptococcus pyogenes with a half-life of 0.9 hours. all of the others (propionibacterium acnes, staphylococcus epidermidis, staphylococcus aureus, and klebsiella pneumoniae) had half-lives o ...	1979	93373
adjvant treatment of tongue and floor of the mouth cancers.	since january 1974, 95 patients with anterior tongue and floor of the mouth cancers were included in a randomized trial. after stratification according to staging and initial treatment, one-third of the patients received chemotherapy for 2 years (methotrexate 400 mg followed by citrovorum factor 100 mg + bleomycin 60 mg/week, during the first 15 weeks), one-third of the patients received immunotherapy with weekly c. parvum injections during 2 years, while the remaining third did not receive any ...	1978	88754
chemotherapy of advanced head and neck cancer with methotrexate, bleomycin, and cis-diamminedichloroplatinum ii in an effective outpatient schedule.	thirty-one patients with advanced cancer of the head and neck, twenty-six of whom had failed prior irradiation, were treated with an out-patient chemotherapy regimen combining methotrexate, bleomycin, and cis-diamminedichloroplatinum (ii). the overall response rate among evaluable patients was 61%, with 22.5% complete remission. if 6 additional patients not completing 3 weeks of treatment are included, the response rate was 51.4%. the median duration of partial remission was 3 months, but none o ...	1979	88256
precursors of the blood group mn antigens as human carcinoma-associated antigens.		1979	88088
immunotherapy models in experimental animals.		1979	87018
effect of propionibacterium acnes on the cellular immune responses to tumor-specific antigens on malignant rat liver cells.	the effect of propionibacterium acnes on the cellular immune responses to tumor-specific membrane antigens was investigated by microcytotoxicity assays (ma) and 51cr release assays (cra) with use of mesenteric lymph node cells (lnc) of syngeneic bd iv and bd vi rats. bd rat liver cell lines transformed in vitro by chemical carcinogens were used as target cells with tumor-specific antigens. by ma, the lnc from rats that were inoculated with malignant liver cells under the adjuvant effect of heat- ...	1979	86635
evidence for the presence of an antitumor factor in serum of normal animals.	we have previously reported finding a factor with antitumor activity (tnf, tumor necrosis factor) in extracts of serum from normal mice. the possibility that tnf exists in the blood of normal animals of other species was explored. horse, mouse, dog, human, sheep, calf, rat and shark serums were fractionated with (nh4)2so4 and filtered through s-200 sephacryl gel. proteins of molecular weight 90,000 to 180,000 were pooled, concentrated and dialyzed. tnf, determined by l-cell assay in vitro and me ...	1979	86384
[immunostimulation].		1978	84450
treatment of acne vulgaris with topically applied erythromycin and tretinoin.	a twice daily application of 2% erythromycin base in hydroalcoholic solution accompanied by once daily use of 0.05% tretinoin (retinoic acid) solution was substantially more effective than tretinoin or erythromycin alone for treatment of inflammatory acne of moderate severity. therapeutic enhancement by this combination can be attributed to the different modes of action, erythromycin acting chiefly by suppressing propionibacterium acnes, while tretinoin is comedolytic. in addition, by altering t ...	1978	83088
malignant angioendothelioma--effect of immunotherapy with corynebacterium parvum.		1978	83086
corynebacterium parvum and hyperthermia.		1978	82029
possible mechanisms underlying the induction of cytotoxic macrophages by corynebacterium parvum: in vitro induction of cytotoxicity in normal macrophages by immune lymphocytes.		1978	81874
role of mononuclear infiltrating cells in pathogenesis of hepatitis.	mice that have been injected with corynebacterium parvum have mononuclear-cell infiltrates in the liver lobules. in such mice a small dose of lipopolysaccharide endotoxin produced a lethal hepatitis, with high serum-transaminase concentrations, glycogen depletion, and hypoglycaemia. it is suggested that lipopolysaccharide triggers the release from the infiltrating mononuclear cells of factors toxic for hepatocytes. similarly certain parasitic and virus infections and graft-versus-host reactions ...	1978	80531
abscesses of the frontal lobe of the brain secondary to covert dental sepsis.	the bacterial species found in pus aspirated from brain abscesses in two patients were typical of those found in dental sepsis. subsequently apical-root abscesses were demonstrated in the upper jaws of both patients. this evidence strongly suggests that these cerebral abscesses were secondary to dental sepsis which could have spread from the teeth to the frontal lobes by several possible antaomical pathways.	1978	79867
corynebacterium parvum toxicity.		1976	73819
[progress in medicine in individual presentations, xi. modern forms of therapy in acne].	several pathogenetic factors contribute to the development of acne vulgaris. these include genetic predisposition, hormonal influences, increasing sebaceous secretion, bacterial colonization of the follicle and keratinization defects in the follicular epithelium. modern acne therapy can take specific forms on the basis of recent research on pathogenesis. sebostatic therapy can be performed by the topical application of benzoyl peroxide or the systemic administration of hormones (oestrogens, anti ...	1977	73259
immunochemotherapy in 34 cases of oat cell carcinoma of the lung with 19 complete responses.	thirty-four previously untreated patients with oat cell carcinoma of the lung were treated with a myelotoxic combination of cyclophosphamide, adriamycin, methotrexate, ccnu, and corynebacterium parvum (regimen a) every 4 weeks, interspersed with a non-myelotoxic combination including bleomycin, vincristine, dehydroemetine, and corynebacterium parvum (regimen b) weekly the other 3 weeks or when hematologic toxicity prohibited administration of regimen a. hematologic toxicity was frequent but was ...	1977	68827
studies on the regulation of lymphocyte reactivity by normal and activated macrophages.		1977	67900
immunotherapy of human cancer.		1977	65906
aldolases of the lactic acid bacteria. demonstration of immunological relationships among eight genera of gram positive bacteria using an anti-pediococcal aldolase serum.	reciprocal qualitative and quantitative immunological experiments employing an anti-pediococcus cerevisiae aldolase serum confirmed many of the interspecific relationships demonstrated previously among lactic acid bacteria with antisera prepared against the streptococcus faecalis fructose diphosphate aldolase. the extent of immunological relatedness observed between the lactobacillus and pediococcus aldolases was markedly gses indicating that the pediococci share closer phylogenetic ties with th ...	1976	65160
[recent personal results in advanced bronchial cancer. palliative immuno-chemotherapy. 63 cases].		1976	64125
utilization of lactose and production of corrinoids in selected strains of propionic acid bacteria in cheese-whey and casein media.	comparative studies were carried out with 23 strains (14 species) of propionibacteria in two media-cheese-whey and casein. the degree of lactose fementation and the efficiency of the corrinoids synthesis were studied. lactose fermentation showed great differences even within one species (e.g. 13.3% and 66.1% for various strains of p. shermanii). the differences were particularly sharp in casein medium (0% or 100%). the highest capacity for utilizing cheese-whey lactose (70--80%) was found in two ...	1976	62496
symptoms from corynebacterium parvum.		1976	61475
the effect of corynebacterium parvum therapy on immunoglobulin class and igg subclass levels in cancer patients.	detailed serological studies have been undertaken in a small group of cancer patients receiving nonspecific immunotherapy with corynebacterium parvum (c. parvum). these patients included 4 cases of recurrent malignant melanoma, 2 of stomach cancer and 2 of recurrent breast cancer. they all received an initial i.v. infusion of 20 mg of a formol killed suspension of c. parvum followed by 2 mg (i.m.) at weekly intervals for 10-11 weeks. this protocol consistently resulted in an increase in the circ ...	1975	61040
editorial: immunostimulation.		1976	60577
metronidazole and acne.	the strong activity of metroindazole against obligate anaerobes suggested that the organism proprionobacterium acnes and the disease acne vulgaris might respond to treatment with this drug. thirty-three clinical isolated of p. acnes were tested and found to be highly resistant to metronidazole. prospects for its successful use in acne are remote.	1976	60028
study of elastolytic activity propionibacterium acnes and staphylococcus epidermis in acne vulgaris and in normal skin.	histopathological sections of anetoderma-like scars from 10 patients with acne vulgaris showed a selective absence of elastic fibers around pilosebaceous follicles. this finding is similar to the histologic changes of "perifollicular elastolysis" reported by varadi. bacteria isolated by anaerobic and aerobic cultures of swabs of the skin surface and pus of these 10 patients, 12 others with active acne vulgaris and 8 normal subjects were studied with particular attention to staphylococcus epiderm ...	1976	60023
biological purpose of acne.	it is argued that the close endocrine control of human sebaceous glands and the likely effects of acne on selection indicate that both gland and disease have persisted because they are biologically useful. two possibilities are proposed: sebum may provide precursor substrates for metabolism and synthesis by the epidermis of compounds of both local and systemic importance; and the adjuvent properties of corynebacteria which colonise the active sebaceous glands of acne may facilitate certain gener ...	1976	58315
analysis of anaerobic coryneform cell wall antigens by radioimmunoassay.		1975	56300
[inhibition of antigenic competition by immunostimulants].	the diminution of immune response against srbc induced in mice, by a prior injection of hrbc was counteracted by addition of certain immunostimulants to srbc. the intensity of inhibition of antigenic competition was related to the quantity of immunostimulant added to srbc. some immunostimulants (b. abortus, lipopolysaccharide) were more active than others (c. parvum, poly i : c). to inhibit antigenic competition immunostimulant had to be injected after or in mixture with srbc never before.	1975	56240
evidence for an allotypic marker on canine immunoglobulin.		1975	53927
letter: side-effects of intravenous corynebacterium parvum.		1975	53755
letter: toxicity of intravenous corynebacterium parvum.		1975	53574
production of antibodies that bind biotin and inhibit biotin containing enzymes.	methods were developed for the coupling of biotin to bovine serum albumin and bovine gamma-globulin using a water-soluble carbodimide. the use of [14-c]biotin as a tracer allowed quantitation of the incorporation of biotin into the conjugates: 2.55 mol of biotin was incorporated per mol of gamma-globulin and 7-9 mol of biotin was incorporated per mol of serum albumin in different preparations. these conjugates were highly immunogenic in the rabbit and anti-bodies reactive with the biotinyl group ...	1975	49192
letter: immunological control of cancer.		1975	48154
letter: adjuvant contact suppression of experimental tumours.		1975	48032
isolation of genital mycoplasmas from blood obtained shortly after vaginal delivery.	blood obtained from three hundred and twenty-seven women within a few minutes of vaginal delivery was cultured for genital mycoplasmas (mycoplasma hominis and t mycoplasmas). twenty-six (8%) of the women had genital mycoplasmas isolated from their blood. ten women had blood-cultures which contained m. hominis. fifteen women had blood-cultures which contained t mycoplasmas. both m. hominis and t mycoplasmas were isolated from the blood of one woman. only one of two hundred and seventy-three blood ...	1975	47945
letter: corynebacterium parvum and anaesthetics.		1975	47551
letter: anaerobic corynebacteria and anaesthetics.		1975	47129
editorial: immunological control of cancer.		1975	46965
letter: corynebacterium parvum and anaesthetics.		1975	46085
the role of bacteroides melaninogenicus and other anaerobes in periodontal infections.	recent taxonomic and anatomical studies of dental plaque associated with periodontal health and disease have demonstrated that differences in the microbial populations in plaque may be responsible for the initiation and progression of disease. the consistent isolation of large numbers of anaerobic and capnophilic bacteria from the depths of periodontal lesions has suggested an important role for these organisms. bacteria that have been isolated include capnocytophaga (bacteroides ochraceus), oth ...	1979	44923
production of hyaluronidase by propionibacteria from different origins.	114 strains of anaerobic and microaerophilic coryneform bacteria from different origins were investigated for production of free extracellular hyaluronidase (hyaluronate glycanohydrolase, ec 3.2.1.36). a quantitative technique was applied measuring the release of n-acetyl-glucosamine groups from purified human potassium hyaluronate. the strains belonged to the following species: propionibacterium acnes, p. avidum, p. granulosum, p. lymphophilum, the formerly so-called corynebacterium parvum, p. ...	1979	44604
[physiologo-biochemical features of the growth and development of propionibacterium shermanii in egg albumin].	propionibacterium shermanii was shown to be able of growing in such an unusual substrate as egg albumen. incubation of the bacterium in egg albumen results in complete utilization of free carbohydrates in this substrate and accumulation in it of such bacterial metabolites as vitamins b2 and b12, free amino acids, bacterial protein, acetic and propionic acids which favour conservation of egg albumen. the bacterium does not possess proteolytic activity and does not utilize albumen; in the conditio ...	1979	43462
purification and partial characterization of hyaluronate lyase (ec 4.2.2.1) from propionibacterium acnes.	hyaluronidase from propionibacterium acnes has been purified 13,000-fold from the culture supernatant to homogeneity (as determined by polyacrylamide disc gel electrophoresis). the molecular weight of the purified enzyme was 85,110 as determined by gel filtration. the purified enzyme had a ph optimum at 6.4, was stable between ph 5 and 5.8 and was completely inactivated after 15 min at 50 degrees c. preliminary studies suggested that the enzyme is active against chondroitin 4- and 6-sulphates, b ...	1979	43356
[effect of aeration on growth retardation of propionibacterium shermanii at different ph values].		1979	41167
amino acid sequence of the biotinyl subunit from transcarboxylase.	the complete amino acid sequence of the biotinyl subunit from the enzyme transcarboxylase of propionibacterium shermanii has been determined from the structures of overlapping tryptic and cyanogen bromide peptides together with sequenator analysis on the whole subunit. the subunit contains 123 amino acid residues. eleven of nineteen residues in the region of biotin attachment, when compared to pyruvate carboxylase from avian liver (rylatt, d. b., keech, d. b., and wallace, j. c. (1977) arch. bio ...	1979	40985
the microbiology of serous and mucoid otitis media.	one hundred forty-four serous and mucoid effusions were cultured for aerobic bacteria, mycoplasma pneumoniae, and virus. thirty percent of all effusions yielded an unequivocally positive culture for aerobic bacteria. although serous effusions were culture positive as often as mucoid effusions, haemophilus influenzae was isolated predominantly from serous effusions and staphylococcus epidermidis predominantly from mucoid samples. only one of 73 effusions yielded a viral isolate (herpesvirus homin ...	1979	36591
[the bacterial flora of preputial space].	the bacterial flora of the preputial space of 210 healthy males (43 children aged between 2 and 11 years, 137 males between 12 and 60 years and 30 men over 60 years) was determined by smears and cultueres from glans penis, sulcus coronarius and the adjacent prepuce. the results were grouped according to various criteria, e.g. glans covered or uncovered and age of males. differences in the distribution of germs could be found in relationship to age. in the case of an uncovered glans penis the pre ...	1979	35486
intergeneric and intrageneric inhibition between strains of propionibacterium acnes and micrococcaceae, particularly staphylococcus epidermidis, isolated from normal skin and acne lesions.	two hundred and forty-one strains or resident skin bacteria comprising 93 isolates of propionob acterium acnes and 148 of micrococcaceae derived from 36 acne patients and 8 control subjects were screened for their ability to inhibit 32 indicator strains, including 20 strains of p. acnes and 12 strains of staphylococcus epidermidis derived from patients with all grades of acne and from normal skin. fifty-three strains (22%) showed some activity against at least one indicator strain. both broad- a ...	1979	34728
complement activation by propionibacterium acnes and bacteroides melaninogenicus.		1978	33641
the role of bacteria in acne vulgaris: a new approach.		1978	32979
effect of immunomodulators on thymocyte suppressive activity in mice.		1978	28950
bacteriocin-like activities of human dental plaque flora against oral anaerobic microorganisms.		1977	26477
resistance to marek's disease. effect of corynebacterium parvum and marek's tumor cell vaccines on tumorigenesis in chickens.	a study of nonspecific stimulation of the avian immune system with corynebacterium parvum and specific stimulation with marek's tumor cell vaccines revealed that nonspecifically stimulated outbred white leghorn-type cockerels had higher incidences of tumors than did controls. a study of tumor cell cytotoxicity of sera from marek's disease virus exposed birds indicated that humoral factors may play some role in tumor resistance.	1978	24754
investigation of the mechanism of the methylmalonyl-coa mutase reaction with the substrate analogue: ethylmalonyl-coa.	1. ethylmalonyl-coa was found to be a substrate for methylmalonyl-coa mutase from propionibacterium shermanii, the product being mainly (2r)-methylsuccinyl-coa along with some (2s)-diastereoisomer. 2. the relevant 1h-nuclear magnetic resonance signals of methylsuccinic acid and of its dimethyl ester were assigned to the diastereotopic methylene hydrogens using sterospecifically dideuterated specimens of known configuration. 3. 2(-2)h1ethylmalonyl-coa was converted by methylmalonyl-coa mutase in ...	1978	24538
aerobic and anaerobic bacteriology of acute otitis media in children.	tympanocentesis and aerobic and anaerobic cultivation of the middle-ear fluid obtained was performed through one or both tympanic membranes of 62 children with acute otitis media. aerobic bacteria alone, predominantely pneumococcus and hemophilus influenzae, were isolated from 57% of patients; anaerobic organisms alone, most commonly propionibacterium acnes and peptococcus, from 15%. thirteen percent yielded mixtures of aerobes and anaerobes, and several had multiple aerobic or anaerobic agents. ...	1978	22590
purification of methylmalonyl-coa mutase from propionibacterium shermanii using affinity chromatography.	a novel procedure for the purification of methylmalonyl-ca mutase from propionibacterium shermanii has been described which employs affinity chromatography on a column of immobilized vitamin b-12 linked covalently to sepharose. the method has the advantage of being simple and rapid, thus enabling the purification of the enzyme to near homogeneity with good yields.	1977	19074
mechanism of inhibition of immunization with irradiated tumour cells by a large dose of corynebacterium parvum.		1977	18742
the bacteriology of skin cysts.	thirty-nine clinically uninflamed cysts of the three most common varieties, epidermoid cysts, trichilemmal cysts and steatocystoma multiplex were removed under sterile conditions and the contents cultured under aerobic and anaerobic conditions. seventy-three percent of epidermoid cysts grew significant numbers of organisms whereas none of the trichilemmal cysts did so. the organisms found were the common skin commensals, staphylococcus epidermidis biotype i, anaerobic gram positive cocci of the ...	1977	17428
desugarization of egg white by microorganisms.	glucose was eliminated from egg whites, using microorganisms, to prevent melanoidin formation which may damage the product. desugarization was achieved by means of acetobacter xylinum, streptococcus lactis, propionibacterium shermanii, pr. petersonii and propionicacid cocci. optimal conditions of desugarization were found, depending on the physiological characteristics of the above microorganisms. propionibacterium shermanii may be well used to ferment a liquid egg white. these bacteria have no ...	1976	17113
a new large form of transcarboxylase with six outer subunits and twelve biotinyl carboxyl carrier subunits.	a new form of transcarboxylase has been isolated which has a molecular weight of 1,200,000, an s20,w of 26 s, and contains 12 biotinyl groups. transcarboxylase as isolated previously has a molecular weight of 790,000, an s20,w of 18 s, and contains six biotinyl groups. the larger species of enzyme consists of a central hexameric subunit with six dimeric outer subunits attached to it by biotinyl carboxyl carrier proteins, three each at the opposite faces of the central subunits. this larger speci ...	1977	14153
antimicrobial effects of some bis-biguanides on certain bacteria which occur in connection with acne vulgaris.		1976	6710
the biosynthesis of vitamin b12.	the use of 13c-fourier transform nuclear magnetic resonance (f.t.-n.m.r.) has led to the observation that while 8 molecules of 2-13cala are incorporated into vitamin b12 in p. shermanii, 5-13cala labels only seven of the carbon atoms of cyanocobalamin, i.e. one of the amino methyl groups of ala is "lost" in the process. it has also been confirmed that seven of the methyl groups of b12 are derived from 13ch3-enriched methionine and further that the chirality of the gemdimethyl grouping at c12 lab ...	1976	4839