Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [srna (srab) regulate the resistant ability of salmonella enterica serovar enteritidis to egg albumen]. | salmonella species are important food-borne pathogens of human and animal. s. enterica serovar enteritidis is the only serovar that routinely causes human infection through intact egg, the molecular basis of its ability to survive in egg is poorly understood. the importance of post-transcriptional regulation by small non-coding rnas (srna) has recently been recognized. the srnas play diverse physiological roles in stress responses, regulation of metabolism, control of bacterial envelope composit ... | 2010 | 21268901 |
| characterisation of proteins extracted from the surface of salmonella typhimurium grown under spi-2-inducing conditions by lc-esi/ms/ms sequencing. | salmonella enterica has two pathogenicity islands encoding separate type three secretion systems (t3ss). proteins secreted through these systems facilitate invasion and survival. after entry, salmonella reside within a membrane bound vacuole, the salmonella containing vacuole (scv), where translocation of a second set of effectors by the salmonella pathogenicity island 2 (spi-2) t3ss is initiated. spi-2 secretion in vitro can be induced by conditions that mimic the salmonella containing vacuole. ... | 2011 | 21268266 |
| characterization of a single-stranded dna binding protein from salmonella enterica serovar typhimurium lt2. | single-stranded dna-binding protein (ssb) plays an important role in dna metabolism, such as dna replication, repair, and recombination, and is essential for cell survival. we characterized the single-stranded dna (ssdna)-binding properties of salmonella enterica serovar typhimurium lt2 ssb (stssb) by using fluorescence quenching measurements and electrophoretic mobility shift analysis (emsa). analysis of purified stssb by gel filtration chromatography showed a stable tetramer in solution. in fl ... | 2011 | 21267641 |
| resistance to ampicillin, third-generation cephalosporins, ciprofloxacin, co-trimoxazole and azithromycin in clinical isolates of salmonella enterica subsp. enterica from germany: real problem or sporadic circumstance? | the objective of this study was to determine the in vitro activity of ampicillin, third-generation cephalosporins, ciprofloxacin, co-trimoxazole and azithromycin against salmonella enterica isolates. none of the isolates tested showed resistance to third-generation cephalosporins or azithromycin. the rates of resistance to ampicillin, co-trimoxazole and ciprofloxacin were 16.8%, 3.2% and 0.8%, respectively. moreover, 7.2% of the isolates showed reduced ciprofloxacin susceptibility, but none of t ... | 2011 | 21265586 |
| rapid and highly efficient method for scarless mutagenesis within the salmonella enterica chromosome. | direct manipulation of bacterial chromosomes by recombination-based techniques has become increasingly important for both cognitive and applied research. here we demonstrate, for the first time, the combination of the red recombinase system with i-scei endonuclease-based selection of successful recombinants after electroporation with short synthetic oligonucleotides. we show the generation of scarless gene knockouts as well as site-directed mutagenesis using the salmonella virulence-associated t ... | 2011 | 21264289 |
| resistance and tolerance to tropodithietic acid, an antimicrobial in aquaculture, is hard to select. | the antibacterial compound tropodithietic acid (tda) is produced by bacteria of the marine roseobacter clade and is thought to explain the fish probiotic properties of some roseobacters. the aim of the present study was to determine the antibacterial spectrum of tda and the likelihood of development of tda resistance. a bacterial extract containing 95% tda was effective against a range of human-pathogenic bacteria, including both gram-negative and gram-positive bacteria. tda was bactericidal aga ... | 2011 | 21263047 |
| role of salmonella pathogenicity island 1 protein iacp in salmonella enterica serovar typhimurium pathogenesis. | gram-negative bacteria, including salmonella enterica serovar typhimurium, exploit type iii secretion systems (t3sss) through which virulence proteins are delivered into the host cytosol to reinforce invasive and replicative niches in their host. although many secreted effector proteins and membrane-bound structural proteins in the t3ss have been characterized, the functions of many cytoplasmic proteins still remain unknown. in this study, we found that iacp, encoded by salmonella pathogenicity ... | 2011 | 21263021 |
| cyclopropane fatty acids are involved in organic solvent tolerance but not in acid stress resistance in pseudomonas putida dot-t1e. | bacterial membranes constitute the first physical barrier against different environmental stresses. pseudomonas putida dot-t1e accumulates cyclopropane fatty acids (cfas) in the stationary phase of growth. in this strain the cfab gene encodes the main cyclopropane synthase responsible of the synthesis of cfas, and its expression is mediated by rna polymerase with sigma factor σ(38). we generated a cfab mutant of p. putida dot-t1e and studied its response to solvents, acid ph and other stress con ... | 2009 | 21261919 |
| development of an oligonucleotide microarray method for salmonella serotyping. | adequate identification of salmonella enterica serovars is a prerequisite for any epidemiological investigation. this is traditionally obtained via a combination of biochemical and serological typing. however, primary strain isolation and traditional serotyping is time-consuming and faster methods would be desirable. a microarray, based on two housekeeping and two virulence marker genes (atpd, gyrb, flic and fljb), has been developed for the detection and identification of the two species of sal ... | 2008 | 21261872 |
| characterization of the rcsc sensor kinase from erwinia amylovora and other enterobacteria. | rcsc is a hybrid sensor kinase which contains a sensor domain, a histidine kinase domain, and a receiver domain. we have previously demonstrated that, although the erwinia amylovora rcsc mutant produces more amylovoran than the wild-type (wt) strain in vitro, the mutant remains nonpathogenic on both immature pear fruit and apple plants. in this study, we have comparatively characterized the erwinia rcsc and its homologs from various enterobacteria. results demonstrate that expression of the erwi ... | 2011 | 21261468 |
| characterization of a t5-like coliphage, spc35, and differential development of resistance to spc35 in salmonella enterica serovar typhimurium and escherichia coli. | the potential of bacteriophage as an alternative biocontrol agent has recently been revisited due to the widespread occurrence of antibiotic-resistant bacteria. we isolated a virulent bacteriophage, spc35, that can infect both salmonella enterica serovar typhimurium and escherichia coli. morphological analysis by transmission electron microscopy and analysis of its 118,351-bp genome revealed that spc35 is a t5 group phage belonging to the family siphoviridae. btub, the outer membrane protein for ... | 2011 | 21257810 |
| detection of aaca-aphd, qaced1, mara, flor, and teta genes from multidrug-resistant bacteria: comparative analysis of real-time multiplex pcr assays using evagreen(®) and sybr(®) green i dyes. | we have developed multiplex real-time pcr assays that utilize dna-intercalating dyes, sybr green i (sg) and evagreen (eg), with two primer sets (set 1=qaced1, teta and aaca-aphd; set 2=teta, mara, and flor) to simultaneously amplify the qaced1, teta, aaca-aphd, mara, and flor genes. validity of the multiplex pcr assays was confirmed by testing 83 bacterial isolates, including staphylococcus aureus (28 isolates), enterococcus spp. (17 isolates), salmonella enterica serovar typhimurium (8 isolates ... | 2011 | 21256956 |
| smm-system: a mining tool to identify specific markers in salmonella enterica. | this report presents smm-system, a software package that implements various personalized pre- and post-blastn tasks for mining specific markers of microbial pathogens. the main functionalities of smm-system are summarized as follows: (i) converting multi-fasta file, (ii) cutting interesting genomic sequence, (iii) automatic high-throughput blastn searches, and (iv) screening target sequences. the utility of smm-system was demonstrated by using it to identify 214 salmonella enterica-specific prot ... | 2011 | 21256892 |
| validation of a seminested pcr approach for rapid detection of salmonella enterica subsp. enterica serovar gallinarum. | salmonella enterica subsp. enterica serovar gallinarum (s. gallinarum) is the causative agent of fowl typhoid, one of the major causes of mortality and morbidity on poultry farms. even though it has been substantially eradicated in many developed countries, the disease still remains endemic in central and south america, in africa and in the mediterranean countries of europe. this leads to the routine screening of flocks, mainly by cultivation and serological techniques, which are expensive, as w ... | 2011 | 21256889 |
| gene expression profiles induced by salmonella infection in resistant and susceptible mice. | mouse models have been extensively used to investigate the mechanisms of salmonellosis. however, the role of the hosts' local intestinal responses during early stages of infection remain unclear. in this study, transcript array analysis was employed to investigate regulation of gene expression in the murine intestine following oral challenge with salmonella enterica serovar enteritidis. salmonella resistant c3h/hen mice elicited only weak transcription responses in the ileum even in the presence ... | 2011 | 21256241 |
| remodelling of the vibrio cholerae membrane by incorporation of exogenous fatty acids from host and aquatic environments. | the gram-negative bacteria vibrio cholerae poses significant public health concerns by causing an acute intestinal infection afflicting millions of people each year. v. cholerae motility, as well as virulence factor expression and outer membrane protein production, has been shown to be affected by bile. the current study examines the effects of bile on v. cholerae phospholipids. bile exposure caused significant alterations to the phospholipid profile of v. cholerae but not of other enteric patho ... | 2010 | 21255114 |
| immunological, physiological, and behavioral effects of salmonella enterica carriage and shedding in experimentally infected finishing pigs. | finishing pigs infected with salmonella pose significant food safety risks by carrying the pathogen into abattoirs. this study was conducted to determine the dynamics of salmonella infection in finishing pigs, and associated immunological, physiological, and behavioral alterations, by longitudinally comparing infected to noninfected pigs during 6 weeks postinfection (p.i.). bacteriological data revealed that all inoculated pigs started shedding salmonella within 2 h p.i., and persistently shed t ... | 2011 | 21254892 |
| application of phage typing and pulsed-field gel electrophoresis to analyse salmonella enterica isolates from a suspected outbreak in lagos, nigeria. | inadequate potable water supply and poor sanitation predispose to food- and water-borne diseases associated with salmonella enterica serovars in developing countries. in this study the possible source of an unprecedented upsurge of salmonella-associated community gastroenteritis was traced using both phage-typing and pulsed-field gel electrophoresis (pfge). | 2010 | 21252464 |
| antimicrobial resistance in salmonella enterica serovar enteritidis in morocco. | salmonella enterica is recognised worldwide as one of the major agents of human gastrointestinal infections. the aim of the present work is to ascertain the antimicrobial susceptibilities of 150 salmonella enterica serovar enteritidis isolates from humans in morocco during the period from 2000 to 2008. | 2010 | 21252460 |
| salmonella enterica serovars typhi and paratyphi a are avirulent in newborn and infant mice even when expressing virulence plasmid genes of salmonella typhimurium. | salmonella enterica serovars typhi and paratyphi a are human host-restricted pathogens. therefore, there is no small susceptible animal host that can be used to assess the virulence and safety of vaccine strains derived from these salmonella serovars. however, infant mice have been used to evaluate virulence and colonization by another human host-restricted pathogen, vibrio cholerae. | 2010 | 21252450 |
| tola mediates the differential detergent resistance pattern between the salmonella enterica subsp. enterica serovars typhi and typhimurium. | the tol-pal genes are essential for maintaining the outer membrane integrity and detergent resistance in various gram-negative bacteria, including salmonella. the role of tola has been well established for the bile resistance of salmonella enterica subsp. enterica serovar typhimurium. we compared the bile resistance pattern between the s. enterica serovars typhi and typhimurium and observed that typhi is more resistant to bile-mediated damage. a closer look revealed a significant difference in t ... | 2011 | 21252278 |
| neonatal lipopolysaccharide exposure impairs sexual development and reproductive success in the wistar rat. | we investigated, in rats, whether neonatal exposure to bacterial lipopolysaccharide (lps) impairs sexual development, sexual decline, and reproductive behaviour in later life. rats were administered either lps (salmonella enterica, serotype enteritidis, 0.05 mg/kg, ip) or saline (equivolume) on days 3 and 5 postpartum. the immediate and long-term effect of treatment on hpa and hpg hormones, testicular morphology, and mating behaviour was assessed. neonatal lps exposure induced a significant incr ... | 2011 | 21251974 |
| cyclic diguanylate signaling proteins control intracellular growth of legionella pneumophila. | proteins that metabolize or bind the nucleotide second messenger cyclic diguanylate regulate a wide variety of important processes in bacteria. these processes include motility, biofilm formation, cell division, differentiation, and virulence. the role of cyclic diguanylate signaling in the lifestyle of legionella pneumophila, the causative agent of legionnaires' disease, has not previously been examined. the l. pneumophila genome encodes 22 predicted proteins containing domains related to cycli ... | 2011 | 21249170 |
| effect of stomaching on numbers of bacteria recovered from chicken skin. | stomaching of skin samples releases only slightly more bacteria than a single rinse. successive rinses, however, continue to remove almost as many bacteria as the first rinse. one hypothesis to explain this observation is that relatively violent treatment of skin generates smaller pieces of skin, thus increasing the net surface area and effectively sequestering bacteria in a water film on the skin pieces so that numbers of bacteria suspended in the rinsate do not increase. an experiment was cond ... | 2011 | 21248349 |
| evaluation of the immunogenicity and biological activity of the citrobacter freundii vi-crm197 conjugate as a vaccine for salmonella enterica serovar typhi. | typhoid fever remains a major health problem in developing countries. young children are at high risk, and a vaccine effective for this age group is urgently needed. purified capsular polysaccharide from salmonella enterica serovar typhi (vi) is licensed as a vaccine, providing 50 to 70% protection in individuals older than 5 years. however, this vaccine is ineffective in infants. vi conjugated to a carrier protein (i.e., an exoprotein a mutant from pseudomonas aeruginosa [repa]) is highly immun ... | 2011 | 21248155 |
| new insights into the mode of action of the actin adp-ribosylating virulence factors salmonella enterica spvb and clostridium botulinum c2 toxin. | the c2 toxin from clostridium botulinum represents the prototype of the family of binary actin-adp-ribosylating toxins. these toxins covalently transfer adp-ribose from nicotinamide adenine dinucleotide (nad(+)) onto arginine-177 of actin in the cytosol of eukaryotic cells resulting in depolymerization of actin filaments and cell rounding. the c2 toxin consists of two non-linked proteins, the enzyme component c2i and the binding and translocation component c2ii, which delivers c2i into host cell ... | 2011 | 21247657 |
| a rapid and sensitive method to identify and differentiate salmonella enterica serotype typhimurium and salmonella enterica serotype 4,[5],12:i:- by combining traditional serotyping and multiplex polymerase chain reaction. | salmonella enterica subspecies enterica serotype 4,[5],12:i:- is an emerging serovar considered as a monophasic variant of salmonella enterica serotype typhimurium. the antigenic and genetic similarity between salmonella 4,[5],12:i:- and salmonella typhimurium suggests that they may behave in a similar way and represent a comparable threat to public health. as serotyping alone does not necessarily provide for identification of salmonella 4,[5],12:i:- and its differentiation from salmonella typhi ... | 2011 | 21247297 |
| label-free identification of bacterial microcolonies via elastic scattering. | label-free microcolony identification via elastic light scattering was investigated for three different genera: salmonella enterica serovar montevideo, listeria monocytogenes f4244, and escherichia coli dh5α. microcolonies were defined as bacterial colonies in their late-lag phase to early-exponential phase with the diameter range of 100-200 µm. to link biophysical characteristics with corresponding scattering patterns, a phase contrast microscope and a confocal displacement meter were used to m ... | 2010 | 21246511 |
| temporal fluctuation of multidrug resistant salmonella typhi haplotypes in the mekong river delta region of vietnam. | typhoid fever remains a public health problem in vietnam, with a significant burden in the mekong river delta region. typhoid fever is caused by the bacterial pathogen salmonella enterica serovar typhi (s. typhi), which is frequently multidrug resistant with reduced susceptibility to fluoroquinolone-based drugs, the first choice for the treatment of typhoid fever. we used a goldengate (illumina) assay to type 1,500 single nucleotide polymorphisms (snps) and analyse the genetic variation of s. ty ... | 2011 | 21245916 |
| a community effort towards a knowledge-base and mathematical model of the human pathogen salmonella typhimurium lt2. | metabolic reconstructions (mrs) are common denominators in systems biology and represent biochemical, genetic, and genomic (bigg) knowledge-bases for target organisms by capturing currently available information in a consistent, structured manner. salmonella enterica subspecies i serovar typhimurium is a human pathogen, causes various diseases and its increasing antibiotic resistance poses a public health problem. | 2011 | 21244678 |
| structural-dynamical investigation of the znua histidine-rich loop: involvement in zinc management and transport. | comparative homology modelling techniques have been used to model the protein znua from salmonella enterica serovar typhimurium using the 3d structure of the homologous protein from escherichia coli. these two-domain proteins bind one zn(2+) atom, with high affinity, in the inter-domain cleft and possess a histidine-rich loop in the n-terminal domain. alternative structures of the znua histidine-rich loop, never resolved by the x-ray diffraction method, have been modelled. a model of the apo for ... | 2011 | 21240623 |
| bistability in myo-inositol utilization by salmonella enterica serovar typhimurium. | the capability of salmonella enterica serovar typhimurium strain 14028 (s. typhimurium 14028) to utilize myo-inositol (mi) is determined by the genomic island gei4417/4436 carrying the iol genes that encode enzymes, transporters, and a repressor responsible for the mi catabolic pathway. in contrast to all bacteria investigated thus far, s. typhimurium 14028 growing on mi as the sole carbon source is characterized by a remarkable long lag phase of 40 to 60 h. we report here that on solid medium w ... | 2011 | 21239589 |
| molecular epidemiology of salmonella enterica serovar typhimurium isolates from cattle in hokkaido, japan: evidence of clonal replacement and characterization of the disseminated clone. | the molecular epidemiology of 545 salmonella enterica serovar typhimurium isolates collected between 1977 and 2009 from cattle in hokkaido, japan, was investigated using pulsed-field gel electrophoresis (pfge). nine main clusters were identified from 116 pfge patterns. cluster i comprised 248 isolates, 243 of which possessed a sequence specific to definitive phage type 104 (dt104) or u302. the cluster i isolates were dominant in 1993 to 2003, but their numbers declined beginning in 2004. beginni ... | 2011 | 21239560 |
| diversity of multidrug-resistant salmonella enterica strains associated with cattle at harvest in the united states. | the prevalence and diversity of multidrug-resistant (mdr) salmonella enterica strains associated with cattle at harvest in the united states were examined. hides and carcasses of cattle were sampled at processing plants (n = 6) located in four geographically distant regions from july 2005 to april 2006. the mean prevalences of salmonella on hides, preevisceration carcasses (immediately after hide removal), and postintervention carcasses (in the chiller and after the full complement of interventi ... | 2011 | 21239549 |
| oral treatment with saccharomyces cerevisiae strain ufmg 905 modulates immune responses and interferes with signal pathways involved in the activation of inflammation in a murine model of typhoid fever. | salmonella spp. are gram-negative, facultative, intracellular pathogens that cause several diarrheal diseases ranging from self-limiting gastroenteritis to typhoid fever. previous results from our laboratory showed that saccharomyces cerevisiae strain ufmg 905 isolated from 'cachaça' production presented probiotic properties due to its ability to protect against experimental infection with salmonella enterica serovar typhimurium. in this study, the effects of oral treatment with s. cerevisiae 90 ... | 2011 | 21236729 |
| prevalence and characterization of extended-spectrum beta-lactamases-producing salmonella enterica isolates in saragossa, spain (2001-2008). | we analyzed the prevalence of resistance to extended-spectrum cephalosporins (escs) among clinical strains of salmonella enterica collected by the laboratory of clinical microbiology in the university clinical hospital lozano blesa in the region of aragón (spain), for which very few epidemiological information exists. a total of 2,092 strains of s. enterica were identified in stool samples from patients with gastroenteritis. five isolates showed an extended-spectrum beta-lactamase (esbl) phenoty ... | 2011 | 21235396 |
| characterization of salmonella enterica isolates from turkeys in commercial processing plants for resistance to antibiotics, disinfectants, and a growth promoter. | salmonella enterica isolates from turkeys in two commercial processing plants (1 and 2) were characterized for susceptibility to antibiotics, disinfectants, and the organoarsenical growth promoter, 4-hydroxy-3-nitrophenylarsonic acid (3-nhpaa, roxarsone), and it's metabolites, naaso(2) (as(iii)) and na(2)haso(4) • 7h(2)o (as(v)). the 130 salmonella serovars tested demonstrated a low incidence of resistance to the antibiotics gentamicin (gen), kanamycin (kan), sulfamethoxazole (smx), streptomycin ... | 2011 | 21235389 |
| analysis of mechanisms involved in reduced susceptibility to ciprofloxacin in salmonella enterica serotypes typhi and paratyphi a isolates from travellers to southeast asia. | owing to multidrug resistance, quinolones and third-generation cephalosporins are currently used as key antibiotics to combat salmonella organisms. therapy failure due to reduced ciprofloxacin susceptibility has been reported in endemic areas, but also in imported disease. different bacterial resistance mechanisms may result in reduced ciprofloxacin susceptibility. in this study, the presence and expression of different resistance mechanisms resulting in reduced minimum inhibitory concentrations ... | 2011 | 21227657 |
| reduction of salmonella enterica on grape tomatoes using microwave heating. | grape tomatoes were surface inoculated with salmonella enterica serovars typhimurium, senftenburg, kentucky and enteritidis and heated for 10, 20, 30, 40 and 50 s using a household microwave oven at two different power levels (medium and high). following heating, viable counts, temperature measurements and quality measurements were performed on the tomatoes. at high power level, more than 2 log reduction of salmonella enterica was detected on grape tomatoes after 50 s but the texture were damage ... | 2010 | 21227524 |
| an attenuated salmonella enterica serovar typhimurium strain lacking the znuabc transporter induces protection in a mouse intestinal model of salmonella infection. | salmonella enterica serovar typhimurium has long been recognised as a zoonotic pathogen of economic significance in animals and humans. attempts to protect humans and livestock may be based on immunization with vaccines aimed to induce a protective response. we recently demonstrated that the oral administration of a salmonella enterica serovar typhimurium strain unable to synthesize the zinc transporter znuabc is able to protect mice against systemic salmonellosis induced by a virulent homologou ... | 2011 | 21219981 |
| prevalence, antibiotic resistance, and molecular characterization of salmonella serovars in retail meat products. | the prevalence of salmonella was determined in chicken meat (n = 26), beef (n = 49), and pork (n = 56) collected from wholesale markets, retail stores, and traditional markets in seoul, south korea, in 2009. antibiotic resistance was assessed, and the molecular subtypes of salmonella isolates were ascertained using an automated repetitive sequence-based pcr (rep-pcr) system (diversilab). a total of 18 salmonella strains were isolated from 17 of 131 samples: 16 strains from each of 16 samples and ... | 2011 | 21219782 |
| inhibitory activity of cheese whey fermented with kefir grains. | we investigated the chemical and microbiological compositions of three types of whey to be used for kefir fermentation as well as the inhibitory capacity of their subsequent fermentation products against 100 salmonella sp. and 100 escherichia coli pathogenic isolates. all the wheys after fermentation with 10% (wt/vol) kefir grains showed inhibition against all 200 isolates. the content of lactic acid bacteria in fermented whey ranged from 1.04 × 10(7) to 1.17 × 10(7) cfu/ml and the level of yeas ... | 2011 | 21219767 |
| salmonella enterica enteritidis biofilm formation and viability on regular and triclosan-impregnated bench cover materials. | contamination of food contact surfaces by microbes such as salmonella is directly associated with substantial industry costs and severe foodborne disease outbreaks. several approaches have been developed to control microbial attachment; one approach is the development of food contact materials incorporating antimicrobial compounds. in the present study, salmonella enterica enteritidis adhesion and biofilm formation on regular and triclosan-impregnated kitchen bench stones (silestones) were asses ... | 2011 | 21219760 |
| reduction of salmonella enterica contamination on grape tomatoes by washing with thyme oil, thymol, and carvacrol as compared with chlorine treatment. | in recent years, multistate outbreaks of salmonella enterica serovars were traced to tomatoes and resulted in serious economic loss for the tomato industry and decreased consumer confidence in the safety of tomato produce. purified compounds derived from essential oils such as thymol and carvacrol had wide inhibitory effects against foodborne pathogens including salmonella. the objective of this study was to determine the antimicrobial activities of thymol, carvacrol, and thyme oil against salmo ... | 2010 | 21219747 |
| analysis of tomato and jalapeño and serrano pepper imports into the united states from mexico before and during a national outbreak of salmonella serotype saintpaul infections in 2008. | case-control studies conducted during a multistate outbreak of salmonella enterica serotype saintpaul infections in 2008 revealed associations between illness and the consumption of jalapeño peppers, serrano peppers, and tomatoes. traceback investigations of implicated jalapeño and serrano peppers led to farms in tamaulipas and nuevo león, mexico. we conducted a novel analysis of a u.s. food and drug administration database of tomatoes and jalapeño and serrano peppers imported from mexico during ... | 2010 | 21219707 |
| efficacy of an anaerobic swab transport system to maintain aerobic and anaerobic microorganism viability after storage at -80 degrees c. | an amies agar gel swab transport system was evaluated for its ability to maintain bacterial viability and relative quantity after freezing at -80°c. nine american type culture collection (atcc) bacterial strains were used: 3 anaerobic strains (propionibacterium acnes, peptostreptococcus anaerobius, and clostridium sporogenes) and 6 facultative or strict aerobic bacterial strains (stenotrophomonas maltophilia, escherichia coli ([atcc 25922 and atcc 11775], salmonella enterica subsp. enterica sero ... | 2011 | 21217035 |
| effect of desiccation on tolerance of salmonella enterica to multiple stresses. | reducing the available water in food is a long-established method for controlling bacterial growth in the food industry. nevertheless, food-borne outbreaks of salmonellosis due to consumption of dry foods have been continuously reported. previous studies showed that dried salmonella cells acquire high tolerance to heat and ethanol. in order to examine if dehydration also induces tolerance to other stressors, dried salmonella enterica serotype typhimurium cells were exposed to multiple stresses, ... | 2011 | 21216905 |
| nucleoid-associated protein hu controls three regulons that coordinate virulence, response to stress and general physiology in salmonella enterica serovar typhimurium. | the role of the hu nucleoid-associated proteins in gene regulation was examined in salmonella enterica serovar typhimurium. the dimeric hu protein consists of different combinations of its α and β subunits. transcriptomic analysis was performed with cultures growing at 37 °c at 1, 4 and 6 h after inoculation with mutants that lack combinations of hu α and hu β. distinct but overlapping patterns of gene expression were detected at each time point for each of the three mutants, revea ... | 2011 | 21212121 |
| phenotypic and molecular characterization of salmonella enterica serovar sofia, an avirulent species in australian poultry. | salmonella enterica serovar sofia (s. sofia) is often isolated from chickens in australia. however, despite its high frequency of isolation from chicken and chicken meat products, s. sofia is rarely associated with animal or human salmonellosis, presumably because this serovar is avirulent in nature. the objective of this work was to investigate the phenotypic and molecular properties of s. sofia in order to assess its pathogenic potential. our in vivo studies support the observation that this s ... | 2011 | 21212118 |
| multilocus variable-number of tandem-repeats analysis of salmonella enterica serotype gallinarum and comparison with pulsed-field gel electrophoresis genotyping. | salmonella enterica serovar gallinarum is the causative agent of fowl typhoid, a severe disease of poultry, responsible for heavy economic losses. epidemiologic investigation of fowl typhoid significantly benefits from molecular typing tools, rapd and pfge have been proposed for this purpose. pfge, a well established technique, is still the gold standard among typing methods for most bacteria, including salmonella. nevertheless, it has some limitations regarding execution and reproducibility, in ... | 2010 | 21208755 |
| analysis of interactions of salmonella type three secretion mutants with 3-d intestinal epithelial cells. | the prevailing paradigm of salmonella enteropathogenesis based on monolayers asserts that salmonella pathogenicity island-1 type three secretion system (spi-1 t3ss) is required for bacterial invasion into intestinal epithelium. however, little is known about the role of spi-1 in mediating gastrointestinal disease in humans. recently, spi-1 deficient nontyphoidal salmonella strains were isolated from infected humans and animals, indicating that spi-1 is not required to cause enteropathogenesis an ... | 2010 | 21206750 |
| structures of the sep22 dodecamer, a dps-like protein from salmonella enterica subsp. enterica serovar enteritidis. | the crystal structure of sep22, a dna-binding protein from starved cells from salmonella enterica subsp. enterica serovar enteritidis, has been determined in two forms: the native state at 1.25 å resolution and an iron-soaked form at 1.30 å resolution. the sep22 protomers form a dodecameric shell with 23 symmetry and a single iron ion per protomer was found at the ferroxidase centre in the iron-soaked form. along the threefold axes of the 23 symmetry, hydrophilic asp channels that consist of asp ... | 2010 | 21206015 |
| inactivation of salmonella in liquid egg albumen by antimicrobial bottle coatings infused with allyl isothiocyanate, nisin and zinc oxide nanoparticles. | to develop an antimicrobial bottle coating effective at inhibiting the growth of salmonella in liquid egg albumen (egg white) and reduce the risk of human salmonellosis. | 2011 | 21205106 |
| structural and genetic relationships of two pairs of closely related o-antigens of escherichia coli and salmonella enterica: e. coli o11/s. enterica o16 and e. coli o21/s. enterica o38. | the o-antigen is a part of the lipopolysaccharide molecule present in the outer membrane of gram-negative bacteria, and is essential for the full function of the microorganisms. salmonella enterica and escherichia coli are taxonomically closely related species. in this study, the o-antigen structures of s. enterica o16 and o38 and e. coli o11 were determined. salmonella enterica o38 and e. coli o21 were found to have identical o-antigen structures, whereas s. enterica o16 and e. coli o11 had clo ... | 2011 | 21205000 |
| horizontal gene transfer of a colv plasmid has resulted in a dominant avian clonal type of salmonella enterica serovar kentucky. | salmonella enterica continues to be a significant cause of foodborne gastrointestinal illness in humans. a wide variety of salmonella serovars have been isolated from production birds and from retail poultry meat. recently, though, s. enterica subsp. enterica serovar kentucky has emerged as one of the prominent salmonella serovars isolated from broiler chickens. recent work suggests that its emergence apparently coincides with its acquisition of a colv virulence plasmid. in the present study, we ... | 2010 | 21203520 |
| humanized mice are susceptible to salmonella typhi infection. | salmonella enterica serovar typhi is a pathogen that only infects humans. currently, there is no animal model for studying this pathogen. recently, alymphoid rag-2(-/-)/γ(c)(-/-) mice engrafted with human leukocytes, known as humanized mice, have been successfully utilized to develop experimental models for several human-specific viral infections, including hiv, human-like dengue fever and hepatitis c virus. little is known about the usefulness and feasibility of the humanized mouse model for th ... | 2010 | 21200387 |
| antimicrobial resistance among invasive nontyphoidal salmonella enterica isolates in the united states: national antimicrobial resistance monitoring system, 1996 to 2007. | nontyphoidal salmonellae (nts) are important causes of community-acquired bloodstream infection. we describe patterns of antimicrobial resistance among invasive nts in the united states. we compared bloodstream nts isolates with those from stool submitted to the national antimicrobial resistance monitoring system (narms) from 1996 to 2007. we describe antimicrobial resistance among invasive strains by serogroup and serotype. of the 19,302 nts isolates, 17,804 (92.2%) were from stool or blood. of ... | 2011 | 21199924 |
| characterisation of proteins extracted from the surface of salmonella typhimurium grown under spi-2-inducing conditions by lc-esi/ms/ms sequencing. | salmonella enterica has two pathogenicity islands encoding separate type three secretion systems (t3ss). proteins secreted through these systems facilitate invasion and survival. after entry, salmonella reside within a membrane bound vacuole, the salmonella containing vacuole (scv), where translocation of a second set of effectors by the salmonella pathogenicity island 2 (spi-2) t3ss is initiated. spi-2 secretion in vitro can be induced by conditions that mimic the salmonella containing vacuole. ... | 2010 | 21197671 |
| assessment of 2 salmonella enterica serovar typhimurium-based vaccines against necrotic enteritis in reducing colonization of chickens by salmonella serovars of different serogroups. | this study assessed the protective efficacy of oral vaccination with 2 experimental attenuated salmonella typhimurium-vectored vaccines for necrotic enteritis in protecting chickens against intestinal colonization by common serovars of salmonella belonging to the 4 major serogroups affecting chickens. birds were vaccinated orally with 1 × 10⁸ colony-forming units (cfu) of 1 of the vaccine strains χ9241 and χ9352, which express a plasmid-encoded partial recombinant hypothetical protein gene (thp) ... | 2010 | 21197226 |
| medicinal plant extracts with efflux inhibitory activity against gram-negative bacteria. | it was hypothesised that extracts from plants that are used as herbal medicinal products contain inhibitors of efflux in gram-negative bacteria. extracts from 21 plants were screened by bioassay for synergy with ciprofloxacin against salmonella enterica serotype typhimurium, including mutants in which acrb and tolc had been inactivated. the most active extracts, fractions and purified compounds were further examined by minimum inhibitory concentration testing with five antibiotics for activity a ... | 2010 | 21194895 |
| lag phase of salmonella enterica under osmotic stress conditions. | salmonella enterica serovar typhimurium was grown at salt concentrations ranging from 0.5 to 7.5% in minimal medium with and without added osmoprotectant and in a rich medium. in minimal medium, the cells showed an initial decline period, and consequently the definition of the lag time of the resultant log count curve was revised. the model of baranyi and roberts (int. j. food microbiol. 23:277-294, 1994) was modified to take into account the initial decline period, based on the assumption that ... | 2010 | 21193660 |
| foreign travel and decreased ciprofloxacin susceptibility in salmonella enterica infections. | to determine antimicrobial drug resistance patterns, we characterized nontyphoidal salmonella enterica strains isolated in liverpool, uk, january 2003 through december 2009. decreased susceptibility to ciprofloxacin was found in 103 (20.9%) of 492 isolates. the lower susceptibility was associated with ciprofloxacin treatment failures and with particular serovars and phage types often acquired during foreign travel. | 2011 | 21192872 |
| mechanism of bactericidal activity of microcin l in escherichia coli and salmonella enterica. | for the first time, the mechanism of action of microcin l (mccl) was investigated in live bacteria. mccl is a gene-encoded peptide produced by escherichia coli lr05 that exhibits a strong antibacterial activity against related enterobacteriaceae, including salmonella enterica serovars typhimurium and enteritidis. we first subcloned the mccl genetic system to remove the sequences not involved in mccl production. we then optimized the mccl purification procedure to obtain large amounts of purified ... | 2010 | 21189348 |
| the salmonella genomic island 1 is specifically mobilized in trans by the inca/c multidrug resistance plasmid family. | the salmonella genomic island 1 (sgi1) is a salmonella enterica-derived integrative mobilizable element (ime) containing various complex multiple resistance integrons identified in several s. enterica serovars and in proteus mirabilis. previous studies have shown that sgi1 transfers horizontally by in trans mobilization in the presence of the inca/c conjugative helper plasmid pr55. | 2010 | 21187963 |
| penetration and activation of brain endothelium by salmonella enterica serovar typhimurium. | salmonella meningitis is a serious disease of the central nervous system, common particularly in africa. here, we show that salmonella enterica serovar typhimurium is able to adhere, invade, and penetrate human brain microvascular endothelial cells (hbmecs), the single-cell layer constituting the blood-brain barrier (bbb). cellular invasion was dependent on host actin cytoskeleton rearrangements, while expression of a functional type iii secretion system was not essential. in addition, salmonell ... | 2010 | 21186258 |
| eukaryotic signaling pathways targeted by salmonella effector protein avra in intestinal infection in vivo. | the salmonella avra gene is present in 80% of salmonella enterica serovar strains. avra protein mimics the activities of some eukaryotic proteins and uses these activities to the pathogen's advantage by debilitating the target cells, such as intestinal epithelial cells. therefore, it is important to understand how avra works in targeting eukaryotic signaling pathways in intestinal infection in vivo. in this study, we hypothesized that avra interacts with multiple stress pathways in eukaryotic ce ... | 2010 | 21182782 |
| identification of bacterial dna in noninfectious pleural fluid with a highly sensitive pcr method. | background: bacterial dna due to bacterial translocation has been identified in noninfectious ascitic fluid samples. objective: this study investigated the possible presence of bacterial dna in the pleural fluid of patients with pleural effusions of noninfectious origin, using a highly sensitive pcr-based method. methods: pleural fluid samples from 175 patients (average age ± sd: 69 ± 14 years) with noninfectious pleural effusion (62 transudates, 113 exudates) were analyzed. bacterial dna was de ... | 2010 | 21178326 |
| enhanced recovery of salmonella typhimurium dt104 from exposure to stress at low temperature. | salmonella enterica serovar typhimurium remains an important cause of food-borne infection in the developed world. in order to establish infection within a host, salmonella must survive and recover from a range of environmental stresses. s. typhimurium strain sl1344 is among the most extensively studied pathogenic salmonella strains, while s. typhimurium phage type dt104 is an important type that has been associated with pandemic spread and a high number of food-borne disease outbreaks over the ... | 2010 | 21178168 |
| comparison of salmonella enterica serovar heidelberg isolates from human patients with those from animal and food sources. | seventy-eight salmonella enterica serovar heidelberg isolates from humans were tested for antimicrobial susceptibility, resistance genes, and plasmids and genotyped by pulsed-field gel electrophoresis (pfge). most (88%) contained plasmids, and 47% were resistant to antimicrobials. the overall results were compared to those of previous s. heidelberg studies of food- and animal-related sources, and multiple similarities were observed. | 2010 | 21177888 |
| inca/c plasmids mediate antimicrobial resistance linked to virulence genes in the spanish clone of the emerging salmonella enterica serotype 4,[5],12:i:-. | to broaden knowledge of the molecular bases and genetics of multidrug resistance in clinical isolates of salmonella enterica serotype 4,5,12:i:- belonging to the spanish clone. | 2010 | 21177672 |
| two groups of phenylalanine biosynthetic operon leader peptides genes: a high level of apparently incidental frameshifting in decoding escherichia coli phel. | the bacterial phel gene encodes the leader peptide for the phenylalanine biosynthetic operon. translation of phel mrna controls transcription attenuation and, consequently, expression of the downstream phea gene. fifty-three unique phel genes have been identified in sequenced genomes of the gamma subdivision. there are two groups of phel genes, both of which are short and contain a run(s) of phenylalanine codons at an internal position. one group is somewhat diverse and features different termin ... | 2010 | 21177642 |
| the impact of different housing systems on egg safety and quality. | a move from conventional cages to either an enriched cage or a noncage system may affect the safety or quality, or both, of the eggs laid by hens raised in this new environment. the safety of the eggs may be altered either microbiologically through contamination of internal contents with salmonella enterica serovar enteritidis (salmonella enteritidis) or other pathogens, or both, or chemically due to contamination of internal contents with dioxins, pesticides, or heavy metals. quality may be aff ... | 2011 | 21177467 |
| salmonella typhimurium oral challenge model in mature broilers: bacteriological, immunological, and growth performance aspects. | in this study, salmonella enterica serovar typhimurium challenge models were tested to identify the best conditions under which to perform the experimental infection of 3-wk-old broilers. such a model would be useful to study the efficiency of therapeutic treatments that could take place at the end of the grow-out period. salmonella-free chicks were obtained from a breeder flock vaccinated with salmonella. intestinal maternal immunity was monitored by elisa analyses at 2, 9, and 16 d of age. dat ... | 2011 | 21177444 |
| effect of heating and aging of poultry litter on the persistence of enteric bacteria. | food-borne illnesses have rarely been associated with the reuse of poultry litter as an organic fertilizer and soil amendment in agriculture. yet farming practices in many countries have come under increased scrutiny because of heightened consumer awareness of food safety and environmental issues. this study was conducted to determine whether simple on-farm management practices could improve the microbiological safety of poultry litter. first, the effects of heat and moisture on the survival of ... | 2011 | 21177438 |
| untangling metabolic and communication networks: interactions of enterics with phytobacteria and their implications in produce safety. | recent outbreaks of vegetable-borne gastrointestinal illnesses across the globe demonstrate that human enteric pathogens can contaminate produce at any stage of production. interactions of enterics with native plant-associated microbiota influence the microbiological safety of produce by affecting the attachment, persistence and proliferation of human pathogens on plants. supermarket surveys have revealed that bacteria, but not fungi or mechanical damage, promote the growth of salmonella enteric ... | 2010 | 21177108 |
| comparative proteomic analysis of salmonella enterica serovar typhimurium ppgpp-deficient mutant to identify a novel virulence protein required for intracellular survival in macrophages. | the global ppgpp-mediated stringent response in pathogenic bacteria plays an important role in the pathogenesis of bacterial infections. in salmonella enterica serovar typhimurium (s. typhimurium), several genes, including virulence genes, are regulated by ppgpp when bacteria are under the stringent response. to understand the control of virulence genes by ppgpp in s. typhimurium, agarose 2-dimensional electrophoresis (2-de) combined with mass spectrometry was used and a comprehensive 2-de refer ... | 2010 | 21176126 |
| the yfer gene of salmonella enterica serovar typhimurium encodes an osmoregulated lysr-type transcriptional regulator. | a genetic screening for osmoregulated genes allowed us to identify the yfer gene of salmonella enterica serovar typhimurium. the yfer gene product encodes a novel lysr-type transcriptional regulator (lttr), the expression of which decreases when external osmolarity increases. out of the adjacent gene yfeh, yfer modulates expression of several genes that may be required for optimal growth under low osmolarity conditions. | 2010 | 21175741 |
| evolution in a family of chelatases facilitated by the introduction of active site asymmetry and protein oligomerization. | the class ii chelatases associated with heme, siroheme, and cobalamin biosynthesis are structurally related enzymes that insert a specific metal ion (fe(2+) or co(2+)) into the center of a modified tetrapyrrole (protoporphyrin or sirohydrochlorin). the structures of two related class ii enzymes, cbix(s) from archaeoglobus fulgidus and cbik from salmonella enterica, that are responsible for the insertion of cobalt along the cobalamin biosynthesis pathway are presented in complex with their metall ... | 2010 | 21173279 |
| transcriptional and proteomic responses of pseudomonas aeruginosa pao1 to spaceflight conditions involve hfq regulation and reveal a role for oxygen. | assessing bacterial behavior in microgravity is important for risk assessment and prevention of infectious diseases during spaceflight missions. furthermore, this research field allows the unveiling of novel connections between low-fluid-shear regions encountered by pathogens during their natural infection process and bacterial virulence. this study is the first to characterize the spaceflight-induced global transcriptional and proteomic responses of pseudomonas aeruginosa, an opportunistic path ... | 2010 | 21169425 |
| structure of the o-polysaccharide and characterization of the o-antigen gene cluster of salmonella enterica o53. | the o-polysaccharide (o-antigen) of salmonella enterica o53 was isolated by mild acid degradation of the lipopolysaccharide and studied by sugar analysis and (1)h and (13)c nmr spectroscopy before and after o-deacetylation. the following structure of the tetrasaccharide o-unit was established: sequencing of the o-antigen gene cluster of s. enterica o53 revealed 11 open reading frames having the same transcriptional direction. the functions were tentatively assigned to the genes based on their si ... | 2010 | 21168829 |
| structures of the o-polysaccharides of salmonella enterica o59 and escherichia coli o15. | the o-polysaccharide of salmonella enterica o59 was studied using sugar analysis and 2d (1)h and (13)c nmr spectroscopy, and the following structure of the tetrasaccharide repeating unit was established: →2)-β-d-galp-(1→3)-α-d-glcpnac-(1→4)-α-l-rhap-(1→3)-β-d-glcpnac-(1→ accordingly, the o-antigen gene cluster of s. enterica o59 includes all genes necessary for the synthesis of this o-polysaccharide. earlier, another structure has been reported for the o-polysaccharide of salmonella arizonae (s. ... | 2010 | 21167479 |
| continuous control of flagellar gene expression by the σ28-flgm regulatory circuit in salmonella enterica. | the flagellar genes in salmonella enterica are expressed in a temporal hierarchy that mirrors the assembly process itself. the σ(28)-flgm regulatory circuit plays a key role in controlling this temporal hierarchy. this circuit ensures that the class 3 genes are expressed only when the hook-basal body (hbb), a key intermediate in flagellar assembly, is complete. in this work, we investigated the role of the σ(28)-flgm regulatory circuit in controlling the timing and magnitude of class 3 gene expr ... | 2010 | 21166907 |
| effect of e-beam treatment on the safety and shelf life of mayonnaise potato salad. | the radioresistance of listeria monocytogenes, salmonella enterica serovar enteritidis, and s. enterica serovar typhimurium has been studied in a complex matrix like mayonnaise potato salad. d(10)-values of 0.56, 0.32-0.35, and 0.41-0.42 kgy were calculated for each organism, respectively. keeping in mind these values, the microbiological criteria, the characteristics of the microorganisms, and a shelf life of the products of 20 days stored at 4°c, an irradiation treatment of 1 kgy was calculate ... | 2010 | 21166582 |
| inactivation of salmonella in organic soil by cinnamaldehyde, eugenol, ecotrol, and sporan. | salmonella can survive in soil for months to years; consequently, soil can be a preharvest source of contamination of produce. elimination of salmonella with natural products and processes such as essential oils is important to prevent infection among consumers. essential oils (distilled extract from plants) have been mainly evaluated in liquid medium and foods in which minimum inhibitory concentration is determined. however, there are no reports describing the impact of essential oils in soil, ... | 2010 | 21166519 |
| salmonella serovars from foodborne and waterborne diseases in korea, 1998-2007: total isolates decreasing versus rare serovars emerging. | salmonella enterica has been one of the most widespread foodborne pathogens in korea. between 1998 and 2007, a total of 9,472 salmonella isolates were identified from foodborne and waterborne illness patients. during that time, korea was transitioning into a developed country in industry as well as in its hygiene system. although the isolation number of total salmonella including serovar typhi has decreased since 1999, the isolation of rare salmonella serovars has emerged. three most prevalent s ... | 2010 | 21165281 |
| high prevalence of plasmid-mediated quinolone resistance determinant aac(6')-ib-cr amongst salmonella enterica serotype typhimurium isolates from hospitalised paediatric patients with diarrhoea in china. | in this study, the antimicrobial susceptibilities and prevalence of plasmid-mediated quinolone resistance determinants amongst salmonella enterica serotype typhimurium isolates from hospitalised paediatric patients with diarrhoea in china were investigated. in total, 40 (64.5%) of 62 s. typhimurium isolates were resistant to ciprofloxacin (minimum inhibitory concentration ≥0.5 μg/ml), comprising 28 isolates with low-level resistance and 12 isolates with high-level resistance. all ciprofloxacin-r ... | 2010 | 21163630 |
| sub-clinical infection with salmonella in chickens differentially affects behaviour and welfare in three inbred strains. | 1. much evidence exists detailing how animals respond to pathogen challenge, yet information explaining how the various behavioural, immunological, and physiological systems in chickens interplay during such challenges remains limited. 2. to gain an understanding of this interplay while controlling for genetic variation, the current study collected a variety of behavioural, physiological and immunological measures from three inbred lines (p, o and n) of laying hens before and after a sub-clinica ... | 2010 | 21161776 |
| enhancement of immune responses by an attenuated salmonella enterica serovar typhimurium strain secreting an escherichia coli heat-labile enterotoxin b subunit protein as an adjuvant for a live salmonella vaccine candidate. | a plasmid harboring eltb, the gene encoding heat-labile enterotoxin (ltb), was constructed by insertion of eltb into an asd(+) β-lactamase signal plasmid (pmmp65). this was introduced into the δlon δcpxr δasd salmonella enterica serovar typhimurium strain and designated the ltb adjuvant strain. ltb protein production and secretion from the strain were demonstrated with an immunoblot assay and enzyme-linked immunosorbent assay. the ltb strain was evaluated for enhancement of immunity and protecti ... | 2010 | 21159921 |
| the hyb hydrogenase permits hydrogen-dependent respiratory growth of salmonella enterica serovar typhimurium. | salmonella enterica serovar typhimurium contains three distinct respiratory hydrogenases, all of which contribute to virulence. addition of h(2) significantly enhanced the growth rate and yield of s. typhimurium in an amino acid-containing medium; this occurred with three different terminal respiratory electron acceptors. based on studies with site-specific double-hydrogenase mutant strains, most of this h(2)-dependent growth increase was attributed to the hyb hydrogenase, rather than to the hya ... | 2010 | 21157514 |
| interference of bifidobacterium choerinum or escherichia coli nissle 1917 with salmonella typhimurium in gnotobiotic piglets correlates with cytokine patterns in blood and intestine. | the colonization, translocation and protective effect of two intestinal bacteria - pr4 (pig commensal strain of bifidobacterium choerinum) or ecn (probiotic escherichia coli strain nissle 1917) - against subsequent infection with a virulent lt2 strain of salmonella enterica serovar typhimurium were studied in gnotobiotic pigs after oral association. the clinical state of experimental animals correlated with bacterial translocation and levels of inflammatory cytokines [a chemokine, interleukin (i ... | 2010 | 21155989 |
| bacterial gold sensing and resistance. | gold ions are mobilized and disseminated through the environment and enter into the cells by non-specific intake. to avoid deleterious effect that occurs even at very low concentrations, bacteria such as salmonella enterica and cupriavidus metallidurans use au-specific merr-type transcriptional regulators to detect the presence of these toxic ions, and control the expression of specific resistance factors. in contrast to the related copper sensor cuer, the au-selective metalloregulatory proteins ... | 2010 | 21153861 |
| multilocus variable number tandem repeat analysis for salmonella enterica subspecies. | genomic analysis of salmonella enterica revealed the existence of a variable number of tandem repeats (vntr) at multiple loci. some s. enterica strains are considered as references (typhi ty2, typhi ct18, typhimurium lt2, enteritidis lk5, pt4, and enteritidis 07-2642, and newport). these allowed the selection of markers to develop the genotyping technique, multiple-locus vntr analysis (mlva). these markers were used to discriminate s. enterica isolated from humans, food, or the environment. in t ... | 2010 | 21153561 |
| analysis of salmonella enterica serotype paratyphi a gene expression in the blood of bacteremic patients in bangladesh. | salmonella enterica serotype paratyphi a is a human-restricted cause of paratyphoid fever, accounting for up to a fifth of all cases of enteric fever in asia. | 2010 | 21151879 |
| fad binding by apbe protein from salmonella enterica: a new class of fad-binding proteins. | the periplasmic protein apbe was identified through the analysis of several mutants defective in thiamine biosynthesis and was implicated as having a role in iron-sulfur cluster biosynthesis or repair. while mutations in apbe cause decreased activity of several iron-sulfur enzymes in vivo, the specific role of apbe remains unknown. members of the abpe family include nosx and rnff, which have been implicated in oxidation-reduction associated with nitrous oxide and nitrogen metabolism, respectivel ... | 2010 | 21148731 |
| camp receptor protein (crp) positively regulates the yihu-ysha operon in salmonella enterica serovar typhi. | salmonella enterica serovar typhi (s. typhi) is the aetiological agent of typhoid fever in humans. this bacterium is also able to persist in its host, causing a chronic disease by colonizing the spleen, liver and gallbladder, in the last of which the pathogen forms biofilms in order to survive the bile. several genetic components, including the yihu-ysha genes, have been suggested to be involved in the survival of salmonella in the gallbladder. in this work we describe how the yihu-ysha gene clu ... | 2010 | 21148209 |
| regulation of the acrab multidrug efflux pump in salmonella enterica serovar typhimurium in response to indole and paraquat. | salmonella enterica serovar typhimurium has at least nine multidrug efflux pumps. among these, acrab is constitutively expressed and is the most efficient, playing a role in both drug resistance and virulence. the acrab locus is induced by indole, escherichia coli-conditioned medium, and bile salts. this induction is dependent on rama through the binding sequence in the upstream region of acra that binds rama. in the present study, we made a detailed investigation of the rama and acrab induction ... | 2010 | 21148208 |
| gcpa (stm1987) is critical for cellulose production and biofilm formation on polystyrene surface by salmonella enterica serovar weltevreden in both high and low nutrient medium. | biofilm formation by salmonella is a serious concern in the food-processing industry and the persistence of the organism in biofilms becomes a constant source of contamination. since there is zero tolerance for salmonella in foods, it is important to understand the mechanism of biofilm formation and to prevent the formation. therefore, this study aimed at investigating the biofilm-forming ability of seafood isolates of salmonella enterica serovar weltevreden (s. weltevreden) under two different ... | 2010 | 21147214 |
| comparison of multilocus variable-number tandem repeat analysis and pulsed-field gel electrophoresis in molecular subtyping of salmonella enterica serovars paratyphi a. | salmonella enterica serotype paratyphi a is a highly clonal organism; pulsed-field gel electrophoresis (pfge) is insufficient in discriminating isolates. a multilocus variable-number tandem repeat analysis (mlva) was developed, and its usefulness in discriminating isolates was compared. pfge analysis with xbai and blni discriminated 55 isolates into 14 types, with a discriminatory index (di) of 0.741 (confidence interval [ci], 0.635-0.847). mlva divided the isolates into 23 types, with a di of 0 ... | 2011 | 21146707 |
| effects on salmonella shell contamination and trans-shell penetration of coating hens' eggs with chitosan. | chitosan is a biopolymer with antimicrobial activity and film-forming properties. in this study, the effects on salmonella shell contamination and trans-shell penetration of coating hens' eggs with chitosan was evaluated. a chitosan was selected from eight types (four non-commercial and four commercial) based on its antimicrobial activity against salmonella enterica serovar enteritidis (s. enteritidis). for this purpose, a contact plate method was developed and chitosans were applied at a concen ... | 2010 | 21146239 |
| virulence plasmid interchange between strains atcc 14028, lt2, and sl1344 of salmonella enterica serovar typhimurium. | strains atcc 14028 and sl1344 of salmonella enterica serovar typhimurium are more virulent than lt2 in the balb/c mouse model. virulence plasmid swapping between strains atcc 14208, lt2, and sl1344 does not alter their competitive indexes during mouse infection, indicating that the three plasmids are functionally equivalent, and that their contribution to virulence is independent from the host background. strains atcc 14028 and lt2 are more efficient than sl1344 as conjugal donors of the virulen ... | 2010 | 21145349 |