Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| plasmodium falciparum and p. berghei: detection of sporozoites and the circumsporozoite proteins in the saliva of anopheles stephensi mosquitoes. | sporozoites and free circumsporozoite (cs) protein were stained immunoenzymatically in 1-min saliva samples collected from anopheles stephensi mosquitoes infected with either plasmodium berghei or p. falciparum. the number of sporozoites in 1-min saliva-streak samples significantly increased as the salivary gland index rose from 3+ to 4+. for p. berghei-infected mosquitoes from which saliva had been collected before 30 days postfeed, the median sporozoite counts for 3+ and 4+ gland indexes were ... | 1992 | 1438147 |
| immunization against cerebral pathology in plasmodium berghei-infected mice. | the development of cerebral lesions in plasmodium berghei-infected mice was dependent on the strain of mice and the size of the infectious inoculum. in particular, c57bl/6j mice develop cerebral lesions when infected with low numbers of parasitized erythrocytes. by increasing the number of parasites in the infectious inoculum, the percentage of animals that develop cerebral malaria is decreased. varying degrees of protection against the development of cerebral malaria can be obtained by several ... | 1992 | 1437278 |
| plasmodium berghei: sporozoites are sensitive to human serum but not susceptible host serum. | human complement was activated by rodent malaria, plasmodium berghei, sporozoites through the alternative pathway, as revealed by c3 deposition on sporozoites using the fluorescent antibody technique. sporozoites exposed to fresh human serum decreased in infectivity to hepg2 cells, but those exposed to heated or c3-deficient human serum showed normal infectivity to hepg2 cells. in contrast, c3 deposition was not observed on the sporozoites treated with mouse or rat serum even in the presence of ... | 1992 | 1426138 |
| development of a model ribosomal rna hybridization assay for the detection of sarcocystis and other coccidia. | two regions of the primary structure of the small subunit rrna of sarcocystis muris bradyzoites were compared with nucleotide sequences of s. gigantea, toxoplasma gondii, plasmodium berghei and mus musculus and used to design genus- and species-specific probes for the detection and identification of coccidia. total cellular rna of purified s. muris, s. cruzi, t. gondii and eimeria nieschulzi and coccidia-infected tissues of mouse, ox, sheep and pig, were assayed using twenty-base oligomers label ... | 1992 | 1423056 |
| extensive turnover of telomeric dna at a plasmodium berghei chromosomal extremity marked by a rare recombinational event. | the dynamics of telomere turnover were studied in plasmodium, whose telomeric structures consist of linear, recognisable sequences of two distinct repeats (tttaggg and ttcaggg). independent recombinant clones containing a well-defined chromosomal extremity of plasmodium berghei, both before and after a rare insertion event took place, were obtained from clonal parasite populations and analysed. the insertion, which splits the original telomere and causes a significant reduction in the size of th ... | 1992 | 1408751 |
| synthesis and antimalarial properties of 1-imino derivatives of 7-chloro-3-substituted-3,4-dihydro-1,9(2h,10h)-acridinediones and related structures. | to improve upon the activity and properties of the 3-aryl-7-chloro-3,4- dihydro-1,9(2h,10h)-acridinediones, a variety of 1-[(alkylamino)alkylene]imino derivatives (3) were prepared and shown to be highly active antimalarial agents in both rodents and primates. among structural modifications prepared, including n10-alkyl and c2-substituted analogs, removal of the c9 oxygen, and introduction of an imino side chain at c9, the imines of the n10-h acridinediones were the most active compounds obtaine ... | 1992 | 1404226 |
| [observation of ultrastructure and drug sensitivity of in vitro cultured exoerythrocytic form of plasmodium berghei]. | the ultrastructure of in vitro cultured exoerythrocytic stage (ee) of plasmodium berghei (p. b.) was observed under transmission electron microscope (tem). the drug sensitivity of ee was also measured in vitro. the ee was cultured in monolayer host cell, fixed and embedded in situ. the ultrathin sections were prepared and examined by routine methods. the tem pictures showed that the fine structure of in vitro cultured ee was similar with that of ee grown in rat hepatocytes in vivo, as described ... | 1992 | 1394909 |
| [the blood schizontocidal effects of pyronaridine, amodiaquine, mefloquine and qinghaosu on mice infected with plasmodium berghei]. | the asexual stages of p. berghei anka were completely eliminated as revealed in a "4-day suppressive test" with the daily dose of pyronaridine 12.5 mg base/kg or amodiaquine 25 mg base/kg. mefloquine 25 mg base/kg and qinghaosu 100 mg/kg though exerted obvious suppressive effect, the cure rates were only 50% and 0%, respectively. in treating chloroquine-sensitive p. berghei anka strain pyronaridine exhibited the best therapeutic activity, which was followed by amodiaquine, mefloquine and quingha ... | 1992 | 1394908 |
| t helper epitopes enhance the cytotoxic response of mice immunized with mhc class i-restricted malaria peptides. | we have previously derived mhc class i (h-2kd) restricted cytotoxic t lymphocytes (ctl) from balb/c mice immunized with irradiated sporozoites from plasmodium (p.) berghei and p. yoelii. the ctl recognize synthetic peptides corresponding to a region of the circumsporozoite (cs) protein that is homologous in the two species. in the present study, we have attempted to induce cs-specific ctl by immunization with those peptides in incomplete freund's adjuvant. only a low level ctl response was detec ... | 1992 | 1383348 |
| immunogenic properties of multiple antigen peptide systems containing defined t and b epitopes. | immunization with chemically defined synthetic polymers, multiple ag peptide (map) systems, containing t and b epitopes of the circumsporozoite protein of p. berghei induce high levels of circulating antibodies that are detectable several months after boosting. the anti-map secondary antibody response is characterized by an increase in the levels of circulating igg and a concomitant decrease in the igm levels. in vitro and in vivo experiments indicated that th epitopes included in the map are re ... | 1992 | 1382101 |
| h-2-restricted cytolytic t lymphocytes specific for hla display t cell receptors of limited diversity. | we previously showed that h-2kd-restricted cytotoxic t lymphocyte (ctl) clones specific for a single nonapeptide derived from the plasmodium berghei circumsporozoite (pbcs) protein displayed t cell receptors (tcrs) of highly diverse primary structure. we have now analyzed the tcr repertoire of ctls that recognize a peptide derived from the human class i major histocompatibility complex (mhc) molecule hla-cw3 in association with the same murine class i mhc molecule h-2kd. we first sequenced the t ... | 1992 | 1380061 |
| use of human universally antigenic tetanus toxin t cell epitopes as carriers for human vaccination. | synthetic constructs were assembled as multiple ag peptide systems containing repetitive sequences of plasmodium falciparum and plasmodium berghei, the causative agents of human and murine malaria respectively, and two universal human tetanus toxin t cell epitopes 830-843 and 947-967. these constructs were tested for antibody production in mice and for their capacity to stimulate human pbl and tetanus toxin-specific t cell clones. a high antibody titer can be obtained in mice when multiple ag pe ... | 1992 | 1378079 |
| a protective monoclonal antibody with dual specificity for plasmodium falciparum and plasmodium berghei circumsporozoite proteins. | an igm monoclonal antibody (mab 36) which reacts with the circumsporozoite (cs) proteins of both p. falciparum and p. berghei was isolated from plasmodium falciparum sporozoite-immunized mice. in assays of biological activity, mab 36 induces the cs precipitation reaction with live sporozoites and blocks the invasion of hepatoma cells by sporozoites in vitro at concentrations much lower than those observed for previously reported cs protein-specific monoclonal antibodies. mab 36 also provided com ... | 1992 | 1375561 |
| identification and quantification of rodent malaria strains and species using gene probes. | a dna probe pcsv4 and a subclone thereof pcsv4.1, hybridize specifically to rodent malaria dna. dna purified from a small volume (10 microliters) of infected mouse blood was used to determine the composition of the parasite population present. the hybridization signal following pcsv4 probing of slot-blotted dna correlated directly with parasitaemia. the hybridization pattern and intensity, resulting from probing restriction enzyme digested and southern-blotted genomic dna, determined the identit ... | 1992 | 1359498 |
| most residues on the floor of the antigen binding site of the class i mhc molecule h-2kd influence peptide presentation. | a panel of 15 single alanine substitutions on the floor of the peptide binding groove of the murine class i histocompatibility molecule h-2kd has been analyzed. all but two mutant molecules were expressed on the cell surface, and were tested for peptide binding and presentation to specific cytotoxic t lymphocytes. eleven out of 13 mutant molecules appeared to be functionally altered. five of the substituted residues were involved in the presentation of all peptides tested. three participated in ... | 1992 | 1358191 |
| inhibition of the growth of plasmodium falciparum and plasmodium berghei in vitro by an extract of cochlospermum angolense (welw.). | an extract of cochlospermum angolense (welw.) is used in the traditional medicine of angola for the therapy of icterus and for the prophylaxis of malaria. from the roots of this plant red crystalline substances have been isolated and tested for their effect on plasmodium falciparum in vitro and on the dna and protein synthesis of plasmodium berghei. the multiplication of p. falciparum was decreased to 50% of the control in the presence of 10 micrograms/ml extracted material and there was a total ... | 1992 | 1356304 |
| attenuation of malaria infection, paralysis and lesions in the central nervous system by low protein diets in rats. | young wistar rats developed a fulminant infection when inoculated with the rodent malaria parasite plasmodium berghei. rats that died during the infection exhibited a progressive paralysis of the extremities, a rapidly decreasing body temperature and minute haemorrhages in the brain. increasing the level of protein in the diet from 4 to 8 and 16% was accompanied by an increase in morbidity and mortality from 15 to 40 and 90% respectively on day 6 of the infection. increasing the level of dietary ... | 1992 | 1356299 |
| mini- and micro-satellites in the genome of rodent malaria parasites. | higher eukaryotes contain within their dna numerous arrays of repetitive dna, many of which are known as satellite dnas and display extensive variability. the presence of these repeats has been demonstrated for various species and they have been used for genetic identification and classification. here, it is demonstrated that southern hybridisation of dna from rodent malaria parasites allows detection of micro- and minisatellite sequences in the genome of plasmodium species. closely related line ... | 1992 | 1355061 |
| the role of t cells in pathogenesis and protective immunity to murine malaria. | t-cell-mediated immunity to a virulent strain of plasmodium berghei nk65 (pb nk65) and to an attenuated derivative (pb xat) of the strain were examined in cba mice by the administration of monoclonal antibodies against t-cell subsets or interferon-gamma (ifn-gamma). the injection of anti-cd8+ or anti-ifn-gamma delayed the mortality of mice infected with pb nk65, although it did not affect the parasitaemia. in the late stage of pb nk65 infection, t cells, especially cd8+ t cells, were increased i ... | 1992 | 1350570 |
| antimalarial activity of orotate analogs that inhibit dihydroorotase and dihydroorotate dehydrogenase. | dihydroorotase and dihydroorotate dehydrogenase, two enzymes of the pyrimidine biosynthetic pathway, were purified from plasmodium berghei to apparent homogeneity. orotate and a series of 5-substituted derivatives were found to inhibit competitively the purified enzymes from the malaria parasite. the order of effectiveness as inhibitors on pyrimidine ring cleavage reaction for dihydroorotase was 5-fluoro orotate greater than 5-amino orotate, 5-methyl orotate greater than orotate greater than 5-b ... | 1992 | 1348618 |
| distinct t cell specificities are induced with the authentic versus recombinant plasmodium berghei circumsporozoite protein. | plasmodium berghei sporozoite (spz)-immune lymph node (ln) cells obtained from mice of different h-2 haplotypes were analyzed for the presence of circumsporozoite (cs) protein-reactive t cells in proliferative assays. although lymphocytes from each strain responded in vitro to the priming ag and to the soluble rcs protein, they did not respond to cs protein synthetic peptides. parallel analysis of rcs protein-primed ln cells revealed that the two ag are unequal in generating t cell specificities ... | 1992 | 1348519 |
| interrelationship between schistosomiasis and concomitant diseases. | the biological literature contains many examples of mutual influences between different species of parasites, especially with respect to concomitant helminth infections. several situations are known in which the association of infection by schistosoma mansoni with other pathogens in the same host results in a type of disease which differs from the simple summation of the individual effects of each infection. the present study concerns concomitant infections involving s. mansoni and enterobacteri ... | 1992 | 1343911 |
| deletion, insertion and translocation of dna sequences contribute to chromosome size polymorphism in plasmodium berghei. | extensive chromosome size polymorphism arises in plasmodium berghei during in vivo mitotic multiplication. size differences between homologous chromosomes mainly involve rearrangements in the subtelomeric regions while internal chromosomal regions are more conserved. size differences are almost exclusively due to differences in the copy number of a 2.3 kb subtelomeric repeat unit. not only deletion of 2.3 kb repeats occurs, but addition of new copies of this repeat sometimes results in the forma ... | 1992 | 1343732 |
| immunopathology of malaria: role of cytokine production and adhesion molecules. | 1992 | 1342724 | |
| [a spectrophotometric study of the interaction of neutral red with lysates of erythrocytes infected with plasmodium berghei]. | 1992 | 1340261 | |
| [an evaluation of the effect of phytobacteriomycin on the malarial parasite in the mosquito]. | 1992 | 1331733 | |
| host resistance assays as predictive models in styrene immunomodulation. | three infection models namely an oncogenic virus encephalomyocarditis (emcv), a rodent strain of malaria, plasmodium berghei, and a rodent hookworm parasite, nippostrongylus brasiliensis, were used to confirm the in vivo immunotoxic potential of styrene reported in our previous communication. the altered host resistance to these challenge infections was evaluated in rodents pre-treated with 0, 0.02, 0.03 or 0.05 x ld50 dose of styrene (5 days/week) for 4 weeks. significantly increased mortality ... | 1992 | 1330943 |
| gamete development in plasmodium berghei regulated by ionic exchange mechanisms. | ionic regulation in the induction of exflagellation of plasmodium berghei was investigated by culturing the parasites in various isotonic media. of the salts tested, nahco3 exhibited the highest activity in inducing exflagellation, whereas khco3 showed no activity. in the absence of hco3-, media containing monovalent cation (na+, k+, cs+, rd+, choline+, lysine+, arginine+) and cl- also induced exflagellation, but their activities were lower than that of nahco3. anions of br- or no3- could be sub ... | 1992 | 1329079 |
| [involvement of cellular immunity in pathology. neuromalaria]. | murin cerebral malaria (mcm) with plasmodium berghei anka and the cba/ca mice is the result of an immunopathological process. an overproduction of tnf is implicated in its pathogenesis. recent datas concerning tnf production during the course of plasmodium vinckei vinckei infection, and analysis of relationships between mcm and experimental allergic encephalomyelitis (eae) raise the hypothesis of the involvement of an auto-immune process in the murin disease. the role of cellular immunity in hum ... | 1992 | 1327351 |
| localization of a 230-kd parasitophorous vacuole membrane antigen of plasmodium berghei exoerythrocytic schizonts (lsa-2) by immunoelectron and confocal laser scanning microscopy. | using antiserum to a 230-kd parasitophorous vacuole membrane (pvm) antigen of plasmodium berghei exoerythrocytic schizonts as a specific probe for the pvm, we studied the three-dimensional structure of this membrane within infected host cells by immunoelectron microscopy and confocal laser scanning microscopy at 3, 4, and 50 hr after sporozoite invasion. fluorescent label was not detected at 3 hr, but was associated with the cytoplasm of 24-hr-old exoerythrocytic parasites. specific labeling of ... | 1992 | 1318005 |
| [studies on the antimalarial action of gelatin capsule of artemisia annua]. | the pharmacological and clinical effects of gelatin capsule of artemisia annua (gcaa) were investigated. the results revealed that the ld50 was 162.5 +/- 10.1g (crude drug)/kg and ed50 was 11.9 +/- 2.4g (crude drug) for clearance of parasitemia in mice infected with plasmodium berghei therapeutic index being 13.6, which was 3.5 times more than that of artemisinin. the cure rate of coea for plasmodium berghei and p. vivax infections was 100%, as well as just like that of the extract tablets of ar ... | 1992 | 1303339 |
| spectrophotometric assay of the interaction of plasmodium berghei infected erythrocyte lysates and neutral red. | in order to reveal by absorption spectrophotometry the redox differences between the plasmodium berghei infected erythrocyte lysates (mel) and the healthy ones (hel) we studied their interaction with the neutral red (nr) redox dye. the variation of the dye absorption intensity at 540 nm as a function of the hemoglobin content of the samples was attributed to the redox potential variation of the different hemoglobin aggregates formed in the samples containing different hemoglobin quantities. by s ... | 1992 | 1297467 |
| studies on the infectivity of gametocytes of plasmodium berghei (nk 65) in anopheles stephensi. | the infectivity of gametocytes of plasmodium berghei (nk 65) has been studied in laboratory bred anopheles stephensi. mosquitoes were fed daily on infected male and female mastomys natalensis and subsequent development of the oocysts was monitored in the midguts. maximum number of oocysts were found in mosquitoes which were fed on infected female mastomys on d8 and in male mastomys on d7 post-inoculation. during the next peak of gametocytaemia, very few oocysts developed. these findings suggest ... | 1992 | 1296945 |
| role of free radicals in plasmodium berghei infected mastomys natalensis brain. | lipid peroxide, lipid hydroperoxide, reduced glutathione, oxidised glutathione, lipofuscin contents and the activity of the enzyme superoxide dismutase were assessed in p. berghei infected m. natalensis brain. the results showed significant increase in the levels of lipid peroxides, lipid hydroperoxides and lipofuscin in brain subcellular fractions of p. berghei infected m. natalensis. furthermore, a depressed superoxide dismutase activity was observed along with regulation in glutathione conten ... | 1992 | 1294484 |
| [antimalarial effect of n-hentriacontanol isolated from cuatresia sp (solanaceae)]. | the antimalarial activity of the fatty alcohol, n-hentriacontanol, isolated from the bolivian solanaceae, cuatresia sp, is investigated in vivo through a classical four-day suppressive test against plasmodium berghei and p. vinckei in mice. this product markedly reduced the virulence of experimentally induced p. vinckei infection. n-hentriacontanol belongs to a new class of antimalarial natural compounds to be exploited for therapeutic purposes. | 1992 | 1294019 |
| the chemotherapy of rodent malaria. xlvii. studies on pyronaridine and other mannich base antimalarials. | the activities of mannich base antimalarials, including pyronaridine, have been explored against drug-sensitive (plasmodium berghei n) and chloroquine-resistant (plasmodium yoelii ns) rodent malaria parasites in vivo. lines of these parasites have been developed with resistance to pyronaridine, amodiaquine, or wr 228,258. the responses and patterns of cross-resistance of these lines to mannich bases and other blood schizontocides are inconsistent. it is concluded that some mannich bases may prov ... | 1992 | 1288426 |
| highly diverse t cell recognition of a single plasmodium berghei peptide presented by a series of mutant h-2kd molecules. | we have tested 21 independent ctl clones for recognition of a single peptide derived from the plasmodium berghei circumsporozoite protein in the context of 13 mutants of the murine mhc class i molecule h-2kd. in this series of kd mutants, amino acid residues located on the upper surface of the alpha-helices were individually substituted by alanine. remarkably, most clones displayed individual recognition patterns on the kd mutants. we had previously found that this series of ctl clones was likew ... | 1992 | 1281196 |
| pathology of fatal and resolving plasmodium berghei cerebral malaria in mice. | cba/t6 and balb/c mice inoculated with plasmodium berghei anka strain (pba) died from cerebral malaria 6-8 days post-inoculation. dba/2j mice similarly inoculated developed a non-fatal cerebral malaria, with mild temporary cerebral symptoms, and died between days 15 and 22 from other malaria-related complications. when inoculated with p. berghei k173 (pb) these mouse strains did not develop a cerebral malaria but died between days 15 and 22 from other malaria-related complications. these mouse s ... | 1992 | 1280805 |
| monoclonal antibodies recognize a processing dependent epitope present in the mature cs protein of various plasmodial species. | in the present paper, we have characterized the specificity of a series of monoclonal antibodies (moabs) against plasmodium berghei sporozoites, selected for their lack of reactivity with the repeat domain of the circumsporozoite (cs) protein. we found that these moabs recognize pb44, the mature membrane form of the cs protein, but they do not react with pb54, its precursor. furthermore, these moabs do not react with any of the synthetic peptides representing the linear sequence of the p. berghe ... | 1992 | 1279504 |
| pharmacology of the malaria parasite--a study of dose-response relationships in chloroquine-induced autophagic vacuole formation in plasmodium berghei. | 1975 | 1108883 | |
| difference in antimalarial activity between certain amino alcohol diastereomers. | a striking difference in antimalarial activity between the diastereomers of 6-bromo-alpha-[2-(1-methylpiperidyl)]-9-phenanthrenemethanol, alpha-(3-peperidyl)-3,6-bis(trifluoromethyl)-9-phenanthrenemethanol, and alpha-(3-piperidyl)-2,8-bis(trifluoromethyl)-4-quinolinemethanol was observed. a possible explanation involving the n-o distance and active site binding requirements is suggested. | 1976 | 1107548 |
| synthesis of 2-pyridyl-alpha-toluenesulfonates as antimalarials. | a series of substituted 2-pyridyl-alpha-toluenesulfonates was synthesized for antimalarial testing. they were prepared by treating various 2-pyridinols with alpha-toluenesulfonyl chlorides in the presence of an alkali. in tests against plasmodium berghei in mice at 640 mg/kg, only 3,5-dichloro-2-pyridyl-alpha-toluenesulfonate was considered active, i.e., doubled the mean survival time. | 1975 | 1107516 |
| antimalarial amino alcohols ii: anthraceneaminoethanols and anthraceneaminopropanols (1- and 9-substituted). | the syntheses of seven anthracene amino alcohols with one, two, or three additional substituents are described. these compounds include three 1-aminoethanols, two 9-aminoethanols, and two 9-aminopropanols, prepared from substituted anthraquinones or from 10-chloro-9-anthraldehydes. the antimalarial activity of these compounds, as well as tentative structure-activity relationships, is discussed in the light of previously published work. | 1975 | 1107515 |
| [immunosuppression in protozoan infections]. | 1975 | 1107126 | |
| intramuscular immunization of mice with irradiated plasmodium berghei sporozoites. enhancement of protection with albumin. | 1975 | 1106231 | |
| antimalarials. 10. substituted 3-halo- and 3-methoxy-2-aryl-4-quinoline(di-n-butylaminomethyl)methanols. | four 2-aryl-4-quinoline(di-n-butylaminomethyl)methanols with br, cl, f, or ome in position 3 were synthesized by modifications of standard reactions. the antimalarial activity decreased with increased size of the 3-substituent. the 3-f-4',6,8-cl3 compound was the most active (at 2.5 mg/kg) and was completely curative at 80 mg/kg against p. berghei in mice. | 1975 | 1104832 |
| antimalarials. 3. 3-substituted 1-naphthalenemethanols. | the synthesis and antimalarial activity of 22 3-substituted 1-naphthalenemethanols whose substitution was patterned after the antimalarial 2-substituted 4-quinolinemethanols are described. the compounds were active against plasmodium berghei in mice, the most active being 6-chloro-alpha-(dibutylaminomethyl)-3-(3,4-dichlorophenyl)-1-naphthalenemethanol hydrochloride (3b). the naphthalenemethanols tested, 1b and 2b, were not photosensitizing to albino mice. structure-activity relationships between ... | 1975 | 1104831 |
| antimalarials. synthesis and antimalarial activity of 1-(4-methoxycinnamoyl)-4-(5-phenyl-4-oxo-2-oxazolin-2-yl)piperazine and derivatives. | the preparation and activity against plasmodium berghei of derivatives of 1-(4-methoxycinnamoyl)-4-(5-phenyl-4-oxo-2-oxazolin-2-yl)piperazine are described. replacement of the cinnamoyl group was accomplished by acylation or alkylation of 1-(5-phenyl-4-oxo-2-oxazolin-2-yl)piperazine. modifications of the 5-phenyl group were prepared either by a sequence of reactions involving mandelic ester-pemoline-piperazine pemoline or by the reaction of 5-aryl-2-thio-2,4-oxazolidinedione with piperazine or n ... | 1975 | 1104830 |
| chloroquine resistance in malaria: variations of substrate-stimulated chloroquine accumulation. | the response of [14c]chloroquine accumulation to the provision of substrate was evaluated using washed erythrocytes infected with plasmodium berghei cs (chloroquine-susceptible), with p. berghei cr (chloroquine-resistant), with plasmodium vinckei cs, with p. vinckei cr, or with a strain of p. berghei spontaneously resistant to chloroquine, plasmodium berghei yoelii 17x. erythrocytes infected with chloroquine-resistant parasites had a blunted response, particularly to low glucose concentrations. ... | 1975 | 1104805 |
| evaluation of a method for in vitro ookinete development of the rodent malarial parasite, plasmodium berghei. | 1975 | 1104798 | |
| detection of antibody in white mice infected with plasmodium berghei by means of the indirect immunofluorescence test. | experiments have been conducted in order to test the efficacy of the immunofluorescence test in the detection of antibody in mice infected with plasmodium berghei vincke et zips, 1948. this method enables the detection of antibody in subclinical and latent cases when an occasional parasite only is present in the blood cells. of the various methods used for the preparation of antigen, and of the various fixatives, a satisfactory immunofluorescence reaction was obtained if unfixed erythrocytes wit ... | 1975 | 1104419 |
| effects of lowered environmental temperature on the growth of exoerythrocytic stages of plasmodium berghei. | the effect of lowered host-environmental temperature upon the development and maturation of the preerythrocytic tissue stages of rodent malaria parasites has been investigated in two strains of plasmodium berghei originating from the highlands of katanga. young albino rats inoculated with massive sporozoite doses of p. berghei nk 65 and maintained for 48 hours at 12 degrees c developed small, stunted tissue schizonts, averaging 11 x 15 microns, of a distinct morphology. control rats kept at room ... | 1975 | 1103642 |
| [altered reproductive behaviour of trypanosoma musculi after several years of passage in mice (author's transl)]. | the modified strain multiplied less in pregnant and in plasmodium berghei-infected mice, more in heavily infected normal mice, and more or less in weakly infected normal mice. the discordant behaviour in weakly infected mice was due to the occurrence in some animals of a second phase of more rapid increase of the parasitemia. this behaviour could be seen occasionally in the original strain, too. in inbred mice (balb/chan) the length of parasitemia clearly depended on inoculum size and height of ... | 1975 | 1103390 |
| [course of trypanosoma musculi infections in plasmodium berghi-infected mice (author's transl)]. | trypanosoma musculi brought into plasmodium berghei-infected mice in the later stages of the malaria infection shows rapid, approximately logarithmic multiplication in the peripheral blood. the trypanosome number increases by a factor of 2-9 per day, multiplication of the parasites kills most mice in a few days. many multiplicative forms of the trypanosomes and a few trypanosomes without nuclei are seen in blood smears. histologically and in touch preparations, masses of multiplicative forms of ... | 1975 | 1103389 |
| the nature of age immunity to plasmodium berghei in the rat. | the intensity of plasmodium berghei infections decreases as the age of the rat host increases. the nature of this 'age immunity' was investigated. no experimental support was found for innate resistance involving either serum non-antibody factors or changes in the erythrocytes that inhibit parasites in older rats. a cross reacting immune response active against p. berghei was not found. evidence is presented which shows that rats less than 7 weeks old lack at least part of the functional immunol ... | 1975 | 1103066 |
| t-cell activation in murine malaria. | 1975 | 1102993 | |
| enhanced trypanosoma musculi infections in mice with concomitant malaria. | 1975 | 1102992 | |
| failure to protect mus musculus against plasmodium berghei with footpad injections of killed parasites incorporated in complete freund's adjuvant. | the stimulation of cell-mediated immunity by the injection of antigen from killed parasites incorporated in complete freund's adjuvant did not protect mus musculus from the erythrocytic stages of plasmodium berghei. agglutinating antibodies could not be detected, but delayed hypersensitivity was demonstrable. apparently thymocyte sensitization alone was not sufficient to afford protection. | 1975 | 1102649 |
| neutralizing antibody in rodent malaria. | small numbers of rat erythrocytes infected with viable p. berghei, when inoculated into susceptible rats together with hyperimmune rat serum (his), are fully neutralized. serum from convalescent rats delays the onset of patency but does not neutralize. the neutralizing efficiency of his rises in proportion to the number of successive reinoculations of hyperimmune rats. in contrast, mice inoculated with parasites together with either his or normal rat serum succumbed to the disease at the same ti ... | 1975 | 1101458 |
| antimalarials. 7.2,8-bis(trifluoromethyl)-4-quinolinemethanols. | based on the high antilalarial activity of alpha-(2-piperidyl)-2,8-bis(trifluoromethyl)-4-quinolinemethanol, ten additional 2,8-bis(trifluoromethyl)-4-quinolinemethanols were prepared in which the amino alcohol side chain was structurally varied. synthesis of the compounds is described and antimalarial activity data against plasmodium berghei are presented and discussed in terms of the structure variations. | 1975 | 1100828 |
| mass isolation of anopheles stephensi salivary glands infected with malarial sporozoites. | 1975 | 1100801 | |
| comparison of tritiated hypoxanthine, adenine and adenosine for purine-salvage incorporation into nucleic acids of the malarial parasite, plasmodium berghei. | this study was accomplished to examine the relative importance of different metabolic precursors of nucleic acid synthesis in the malarial parasite, p. berghei. three possible pathways for incorporation of adenine (type) compounds exist: 1) incorporation via hypoxanthine, 2) via adenine, or 3) via adenosine. the parasitized cell and erythrocyte-free malarial parasite were both examined because of possible metabolic differences that could be encountered. hypoxanthine was clearly the best precurso ... | 1975 | 1099747 |
| antimalarial phenanthrene amino alcohols. 3. halogen-containing 9-phenanthrenemethanols. | a series of new 9-phenanthrene amino alcohols has been prepared in which each compound bears from one to five halogen or halogen-containing moieties. a number of these compounds are extremely active against plasmodium berghei in the mouse. some structural requirements for optimal efficacy are considered. | 1975 | 1099200 |
| alterations in the permeability of mouse erythrocytes infected with the malaria parasite, plasmodium berghei. | 1975 | 1099038 | |
| thiosemicarbazones and hydrazones of alpha-methylchalkone as potential chemotherapeutic agents. | the effectiveness of chalkones and derivatives as antibacterial and antifungal agents stimulated our interest in the possibility of coupling this type of compound with certain hydrazines and thiosemicarbazides to determine the potential chemotherapeutic activity of these combinations as anticancer and antimalarial agents. accordingly, 18 hydrazine and thiosemicarbazide derivatives of alpha-methylchalkone (dypnone) have been synthesized for study as potential antitumor agents in animal tumor syst ... | 1975 | 1099024 |
| antimalarials: screening of (orally administered) silver solfonamides against plasmodium berghei. | orally administered silver sulfadiazine is effective against plasmodium berghei. attempts to synthesize an active analogue with antimalarial activity failed. although several of the analogues were chemically stable (like silver sulfadiazine) in sodium chloride, none of the analogues were biologically active. this suggests that the antimalarial activity of silver sulfadiazine is stereo-specific. | 1975 | 1098869 |
| a comparison of pigment from schistosoma mansoni and plasmodium berghei. | the structures of malarial and schistosomal pigment within the parasites are distinct. the two pigments are distinguishable both before and after extraction from host liver. | 1975 | 1098591 |
| the chemotherapy of rodent malaria, xxiii causal prophylaxis, part ii: practical experience with plasmodium yoelii nigeriensis in drug screening. | data are presented on the causal prophylactic action of about 100 compounds of various types against plasmodium yoelii nigeriensis n67 in mice. examples are given to show how action against pre-erythrocytic schizonts may be differentiated from action on emerging erythrocytic stages. in a series of 35 8-aminoquinolines, all but 10 showed definite causal prophylactic activity at tolerated doses. the data permit the compounds to be ranked in order of activity, and many are shown to be more active i ... | 1975 | 1098590 |
| immunological studies in rodent malaria. i: protective immunity induced in mice by mild strains of plasmodium berghei yoelii against a virulent and fatal line of this plasmodium. | mild and virulent lines of plasmodium berghei yoelii are readily distinguished both by their course of infection and by the preference of the virulent line for mature red blood cells. mice given either mild line were fully protected against the virulent p.b. yoelii one week after they had become negative. mice given the mild line of p.b. yoelii 17x were fully protected against a challenge by the virulent line on the third day of infection (d+3). mice given the mild and virulent lines of p.b. yoe ... | 1975 | 1098589 |
| purification and some properties of malarial pigment. | malarial pigment from erythrocytes infected with plasmodium berghei was purified by treatment with sodium dodecyl sulphate solution, followed by incubation with pancreatin. the purified pigment retained the apparently crystalline form of pigment within the parasite, rotated polarised light and had the same solubility characterisation as crude malarial pigment. it contained about 1% iron, all of which could be accounted for in terms of haemin. the iron of the pigment molecule is oxidised by the p ... | 1975 | 1098588 |
| role of complement components in the susceptibility to plasmodium berghei infection among inbred strains of mice. | inbred strains of mice, sensitive and resistant to plasmodium berghei infections, including a complement deficient strain were infected with p. berghei. daily levels of parasitaemia were determined and serum levels of complement components (c3 and c5) measured by standard haemolytic assay. serum levels of c3 and c5 were depressed in all strains of mice infected with p. berghei. there was no difference in the infectivity and course of p. berghei infection in the co-isogenic c5 deficient and non-d ... | 1975 | 1098586 |
| sudden increase in virulence in a strain of plasmodium berghei yoelii. | the mild and chronic 17x strain of plasmodium berghei yoelii showed a sudden increase in virulence following a period of 110 days in the deep freeze. the enhanced virulence was seen in a very high and early parasite peak in the blood and a 100% mortality of all infected mice. the exalted virulence remained unaltered following a number of blood transfers of the strain and after four cyclical transmissions through anopheles stephensi. enzyme pattern studies revealed that the virulent strain posses ... | 1975 | 1098585 |
| the chemotherapy of rodent malaria, xxii. the value of drug-resistant strains of p. berghei in screening for blood schizontocidal activity. | data are provided on the activity of a variety of antimalarial drugs against drug-sensitive and drug-resistant lines of plasmodium berghei in albino mice. parallel data for the response of the drug-sensitive parasites to these compounds in vitro indicate whether the drugs have a chloroquine-like or quinine-like type of action, or neither. the value of this test system for drug evaluation is debated. it is concluded that the in vivo tests do provide a valuable indication of the potential use of a ... | 1975 | 1098584 |
| the chemotherapy of rodent malaria, xxi. action of quinine and wr 122 (a 9-phenanthrenemethanol) on the fine structure of plasmodium berghei in mouse blood. | the effects of quinine and a 9-phenanthrenemethanol, wr 122,455 on the fine structure of plasmodium berghei have been investigated. changes consequent upon quinine treatment were noted in the outer membranes (including those that surround the "food vacuole") and the digestive vacuoles. these changes were followed by cytoplasmic degeneration and vacuolization. wr 122,455 caused morphological changes in at least three areas of the parasite, (1) the outer membranes, (2) the digestive vacuole and (3 ... | 1975 | 1098583 |
| quinoxaline studies. 23. potential antimalarials. substituted 5,8-dimethoxyquinoxalines. | two series of 2,3-disubstituted 5,8-dimethoxy-6-[n- (omega-dimethylaminoalkyl) amino] quinoxalines were prepared: the first series with identical 2,3-substituents h, ch3, c6h5, c6h4-4-cl, and ch2c6h5; and the second with identical styryl groups ch=chc6h5, ch=chc6h4-4-cl, ch=chc6h3-3,4-c12, ch=chc6h4-4-f, ch=chc6h4-4-cf3, and ch=chc6h4-4-no2. none of the substances possessed antimalarial activity; several were toxic at highest dosage levels. | 1975 | 1097693 |
| potential antimalarials. 9. resolution of alpha-diheptylaminomethyl-6-bromo-9-phenanthrenemethanol by an unusual method. | 1975 | 1097687 | |
| synthesis of (4-quinolinoamino)aminoalkyltetrahydronaphthalene derivatives for possible antimalarial activity. | (4-quinolinoamino)aminoalkyltetrahydronaphthalene derivatives were synthesized in an attempt to introduce new agents with antimalarial activity. | 1975 | 1097633 |
| antibody-mediated elimination of malaria parasites (plasmodium berghei) in vivo. | an infective preparation of extracellular blood forms (fp) of plasmodium berghei was used to study some aspects of the interaction between protective antibodies and malaria parasites. fp but not infected erythrocytes (irbc) were shown by the fluorescent antibody technique to be coated by antibodies after in vitro incubation with immune serum. preincubation of both fp and irbc with immune serum followed by their washing did not result in enhanced elimination of the parasites in vivo. however, fp ... | 1975 | 1097338 |
| plasmodium berghei: immunologic enhancement of antigen by adjuvant addition. | 1975 | 1097263 | |
| counter-current immunoelectrophoresis for the demonstration of malarial antigens and antibodies in the sera of rats and mice. | 1975 | 1096380 | |
| anaemia in mice with concomitant schistosoma mansoni and plasmodium berghei yoelii infection. | 1. the effect on anaemia in mice given plasmodium berghei yoelii 3 and 5 weeks after exposure to schistosoma mansoni cercariae, was investigated. 2. haematological criteria (pcv and haemoglobin levels), reticulocytosis, parasitaemia and splenomegaly were used as indices. 3. anaemia was severe in the animals given p. b. yoelii and in those with mixed infection (p. b: yoelii plus s. mansoni). malaria was found to dominate the picture until the clearance of the parasitaemia. the effect of the inter ... | 1975 | 1096378 |
| cerebral malaria in a virulent rodent plasmodial infection. | 1975 | 1096377 | |
| antimalarial compounds. xiv. new derivatives of 2-bromo-n,n-bis-(diethylaminoethyl)-4,5-dimethoxyanaline (rc 12). | the molecule of rc 12 which was found previously to exhibit antimalarial activity has been modified, by replacing groups in positions 1 and 2 by nhar, s-ar, no2, sulfonamido and other substituents. the new derivatives (table 1) were tested for toxicity and antimalarial activity agains plasmodium berghei in mice (table 2). | 1975 | 1096102 |
| antimalarial compounds. xiii. new derivatives of phenyl chloromethyl sulfone. | a series of chloromethylsulfonyl derivatives of amines, biguanides and amidineureas (scheme 1) has been prepared and their antimalarial properties investigated. | 1975 | 1096101 |
| splenic mediated erythrocyte cytotoxicity in malaria. | cell-mediated cytotoxicity in virulent rodent malaria has been demonstrated in vitro, whereby splenic cells effected specific lysis of 51cr-labelled erythrocytes from parasitized animals. more than one cellular cytolytic effector system appeared to be operative in the mouse. one effector system involved splenic macrophages, from normal or immune animals, which were increasingly cytotoxic to target cells in the presence of antibody. a second effector system involved nylonpurified immune spleen ce ... | 1975 | 1095474 |
| circadian and other rhythms of parasites. | 1975 | 1094812 | |
| synthesis and evaluation of 6-arylactamido-2,4-diaminoquinazolines and related compounds as folic acid antagonists. | a series of 2,4-diaminoquinazolines bearing an aryl function attached to the 6 position through an acetamido or related linkage was synthesized. each compound was evaluated as an inhibitor of rat liver dihydrofolate reductase as well as for suppressive antimalarial effects against plasmodium berghei in mice. significant in vivo activity was found to reside primarily with 5-chloro-6-arylacetamido derivatives. most of these compounds were also tested for prophylactic activity against sporozoite-in ... | 1975 | 1094114 |
| synthesis and antimalarial activity of heterocyclic alkyl disulfides, thiosulfates, and dithio acid derivatives. | based on the antimalarial activity in mice of bis(4-rho-acetamidobenzenesulfonamidophenyl) disulfide, a series of n-heterocyclic alkyl disulfides and thiosulfates was synthesized and screened for antimalarial activity. several related dithio acid dianions and s- blocked derivatives were also screened to provide an indication of the possible role that thiol anions might play in malaria chemotherapy. activity was limited by toxicity with these compounds, and none of those tested, with the exceptio ... | 1975 | 1092835 |
| a new prodiginne (prodigiosin-like) pigment from streptomyces. antimalarial activity of several prodiginnes. | two prodigiosin-like pigments from streptomyces sp. were shown to be undecylprodiginine (i) and butylcycloheptylprodiginine (v). the antimalarial activity of five prodiginine pigments is given. | 1975 | 1092639 |
| qualitative analysis of phospholipids isolated from nonviable plasmodium antigen. | 1975 | 1091493 | |
| detailed purine salvage metabolism in and outside the free malarial parasite. | 1975 | 1091492 | |
| rodent systems (plasmodium berghei-anopheles stephensi) for screening compounds for potential causal prophylaxis. | an in vivo screening system is described in which drugs administered to rats or mice and challenged with sporozoites are evaluated for their antimalarial properties (causal prophylaxis, suppression, therapy) by the presence or absence of exoerythrocytic forms and parasitemia. the system is composed of a/j mice, sprague-dawley rats, plasmodium berghei, and anopheles stephensi. good correlation has been found between test results and practical application. | 1975 | 1091166 |
| development of infectivity by the plasmodium berghei sporozoite. | studies were done on the development of infectivity during ontogeny of the sporozoite of the rodent malaria parasite, plasmodium berghei. populations of sporozoites were separated from the oocysts, the hemocoel, and the salivary glands, with special precautions being taken to avoid cross-contamination between the different populations. the results indicated that populations of salivary gland sporozoites were more than 10,000 times as infective as populations of oocyst sporozoites from the same m ... | 1975 | 1090717 |
| humoral immunity in rodent malaria. iii: studies on the site of antibody action. | serum which protects rats against plasmodium berghei infections fails to sensitize parasitized erythrocytes in vitro for in vivo destruction. further, the efflux of 86rb from parasitized erythrocytes in the presence of complement is not accelerated. on administration to animals with preexisting malaria, it does, however, produce a relatively slow decline in parasitemia, a phenomenon interpreted in terms of the gradual production and/or release of reactive antigenic determinants associated with m ... | 1975 | 1090670 |
| the in vitro culture of the blood stages of plasmodium berghei. | 1975 | 1090550 | |
| regional immunosuppression induced by plasmodium berghei yoelii infection in mice. | plasmodium berghei yoelii infection in mice severely depressed the splenic antibody response to sheep erythrocytes but had lettle effect on antibody formation in lymph nodes. | 1975 | 1090525 |
| plasmodium berghei: thyroid hyperplasia and thyroid hyperactivity in mice. | 1975 | 1090441 | |
| motility of plasmodium berghei ookinetes in vitro. | 1975 | 1089734 | |
| inhibition of macromolecular synthesis in the malarial parasites by inhibitors of proteolytic enzymes. | 1975 | 1089545 | |
| structure of aplasmomycin. | a new antibiotic, aplasmomycin, was isolated from a broth cultivated with a marine isolate of actinomycete, and inhibits gram-positive bacteria in vitro and plasmodium berghei in vivo. it is a natural ionophore and the structure of the ag-salt was solved by an x-ray crystallographic analysis. it has symmetric structure having boron in the centre of the molecule. | 1977 | 924893 |