Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [results of bacteriologic and mycologic investigations of otitis media in dogs]. | this study describes the bacteriological and mycological investigation of 212 ear swab samples of dogs with an otitis externa. 11 samples were sterile. malassezia pachydermatis could be cultivated in 72.1% of all samples, in 21.9% of these cases in pure culture. in 27.9% of all samples only bacteria were found. coagulase-positive haemolytic staphylococcus and malassezia pachydermatis were isolated together in 31.8% of all ear swab samples. pseudomonas aeruginosa could be detected in 22.9%, haemo ... | 1997 | 9289887 |
| structure of the o-specific polysaccharide of proteus vulgaris o25 containing 3-o-[(r)-1-carboxyethyl]-d-glucose. | the o-specific polysaccharide of proteus vulgaris o25 was studied by acid hydrolysis and by 1h-nmr and 13c-nmr spectroscopies, including one-dimensional noe and two-dimensional cosy and heteronuclear 13c,1h correlation (hetcor) spectroscopy. the polysaccharide was found to contain 3-o-[(r)-1-carboxyethyl]-d-glucose (d-rglc), and the following structure of the pentasaccharide repeating unit was established: [structure in text] | 1997 | 9288919 |
| negative feedback from a proteus class ii flagellum export defect to the flhdc master operon controlling cell division and flagellum assembly. | the proteus mirabilis flagellum class i flhdc operon was isolated, and its transcript was shown to originate from a sigma70 promoter 244 bp 5' of flhd and 29 bp 3' of a putative cyclic amp receptor protein-binding site. expression of this regulatory master operon increased strongly as cells differentiated into elongated hyperflagellated swarm filaments, and cell populations artificially overexpressing flhdc migrated sooner and faster. a class ii flha transposon mutant was reduced in flagellum cl ... | 1997 | 9287017 |
| an edwardsiella tarda strain containing a mutation in a gene with homology to shlb and hpmb is defective for entry into epithelial cells in culture. | edwardsiella tarda is an enteric pathogen that causes diarrhea, wound infections, and death due to septicemia. this species is capable of invading human epithelial cell lines, and we have now been able to follow the entry and replication of e. tarda within tissue culture host cells. e. tarda escapes from the endocytic vacuole within minutes of entry and then replicates within the cytoplasm. unlike other well-studied bacteria that replicate and reside in the cytoplasm, we never observed this orga ... | 1997 | 9284172 |
| some pathogenic fungi involved in serious cases of urinary tract infections in nigeria. | three hundred early morning midstream urine samples collected from 200 females and 100 males between the ages of 20 and 60 years, suffering from urinary tract infections (uti) were studied to determine the etiologic agents. the caliberated loop-direct method used for culturing each sample on both bacterial and fungal media showed significant microbial growth in 117 of the 300 samples. bacteria were the sole agents of infection in 30% of the cases while pathogenic yeasts were the sole agents of i ... | 1997 | 9282508 |
| carbenicillin-hydrolysing penicillinase mediated by a plasmid of proteus mirabilis and its relationship to the pse-type enzymes of pseudomonas aeruginosa. | the nucleotide sequence of a carbenicillin-hydrolysing (carbenicillinase) gene occurring in an endogenous plasmid, pcs229, of proteus mirabilis was determined. the amino acid sequence of the mature enzyme, comprising 271 amino acids with a molecular mass of 29,506 da, was deduced. the pcs229 carbenicillinase showed only 46.4% similarity, in the overall amino acid sequence, to the chromosomal carbenicillinase of pr. mirabilis gn79; however, the enzyme showed about 98% similarity to a pseudomonas- ... | 1997 | 9281821 |
| rapid, sensitive, microbial detection by gene amplification using restriction endonuclease target sequences. | the use of primers synthesized to eight class ii restriction endonuclease target sequences, from haemophilus parainfluenzae, escherichia coli, staphylococcus aureus, salmonella infantis, rhodobacter sphaeroides, klebsiella pneumoniae, bacillus amyloliquefaciens and proteus vulgaris for single and multiplex pcr identification of the organisms is discussed. results indicate that the method is sensitive and specific enough to detect single cells and attogram amounts of target dna. it has also been ... | 1997 | 9281417 |
| recurrent bacterial meningitis: a 6-year experience in adult patients. | ten adult patients with recurrent bacterial meningitis (rbm) of 22 episodes were diagnosed and treated at the dicle university hospital from january 1990 to december 1995. apart from 22 episodes of rbm these patients had an additional 25 episodes treated at other hospitals. the rbm attacks developed after closed head trauma in four patients, asplenia and chronic otitis media in one patient, chronic otitis media and oto-mastoiditis in one patient, chronic maxillary sinusitis in one patient, chron ... | 1997 | 9279725 |
| eosinophilic interleukin 5 (il-5) transgenic mice: eosinophil activity and impaired clearance of schistosoma mansoni. | eosinophilia is a feature common to many invasive helminth infections and eosinophils are often considered to be effector cells in immunity to helminths. this study examined the possible influence of constitutive eosinophilia on the clearance of schistosoma mansoni infections in mice. eosinophils from interleukin-5 transgenic mice exhibit normal ultrastructure and function with regard to phagocytosis and killing of bacteria and responses to chemotactic stimuli. il-5 transgenics and non-transgeni ... | 1997 | 9278941 |
| structure of the o-specific polysaccharide of the bacterium proteus mirabilis o13 containing a novel component: an amide of d-galacturonic acid with n(epsilon)-(1-carboxyethyl)lysine. | an acidic o-specific polysaccharide was obtained by mild degradation of the lipopolysaccharide of the bacterium proteus mirabilis o13 and found to contain d-galactose, 2-acetamido-2-deoxy-d-glucose, and n(epsilon)-(1-carboxyethyl)-n(alpha)-(d-galacturonoyl)lysine. on the basis of full acid hydrolysis and 1h- and 13c-nmr spectroscopy, including two-dimensional correlation spectroscopy (cosy), h-detected heteronuclear 1h, 13c multi-quantum coherence (hmqc), and rotating-frame nuclear overhauser ef ... | 1997 | 9275291 |
| structure of the acid-labile galactosyl phosphate-containing o-antigen of the bacterium proteus vulgaris ox19 (serogroup o1) used in the weil-felix test. | the structure of the o-specific polysaccharide chain of proteus vulgaris ox19 lipopolysaccharide which determines the o1 specificity of proteus and is used in the weil-felix test for diagnostics of rickettsiosis was established. on the basis of 1h- and 13 c-nmr spectroscopy, including two-dimensional correlation spectroscopy (cosy), h-detected 1h, 13c heteronuclear multiple-quantum coherence (hmqc), and rotating-frame nuclear overhauser effect spectroscopy (roesy), it was found that the polysacc ... | 1997 | 9275285 |
| [neurocutaneous syndromes with vascular alterations]. | there are several syndromes in which neurological and cutaneous alterations of vascular origin, among other symptoms, occur. the key point of this fact is that these cutaneous signs permit early diagnosis, thus helping in further recognition of more complex syndromes and preventing unnecessary, harmful and costly diagnostic procedures or having to wait until the appearance of neurological signs. therefore, these diseases should be classified attending to the most notorious vascular lesions they ... | 1997 | 9273170 |
| structures of the o-antigens of proteus bacilli belonging to ox group (serogroups o1-o3) used in weil-felix test. | structures of the o-specific polysaccharide chains of lipopolysaccharides from proteus group ox strains (serogroups o1-o3) used as antigens in weil-felix test for diagnosis of rickettsiosis, were established. from them, the acid-labile polysaccharide of proteus vulgaris ox19 (o1) is built up of the following branched pentasaccharide repeating units connected via a phosphate group: [structure in text] where quinac stands for 2-acetamido-2,6-dideoxyglucose (n-acetylquinovosamine). the basis of ser ... | 1997 | 9271209 |
| taxonomic changes in tailed phages of enterobacteria. | out of 136 new phages, 80 (59%) are classified into 23 species according to morphology and physicochemical properties. six new species are described and species beta 4, from a previous classification scheme, is renamed t1. the morphology of 36 phage species is schematically represented. | 1997 | 9267450 |
| concentration of cefamandole in plasma and tissues of patients undergoing cardiac surgery: the influence of different cefamandole dosage. | to develop an improved regimen of antibiotic prophylaxis in cardiac surgery, three antibiotic prophylactic regimens for patients scheduled to have elective cardiothoracic surgery involving a median sternotomy were evaluated. | 1997 | 9263086 |
| utility of urine and blood cultures in pyelonephritis. | to determine how often the results of urine and blood cultures led to changes in antibiotic therapy for patients discharged from the hospital with the diagnosis of pyelonephritis. | 1997 | 9262699 |
| cerebrospinal fluid broth culture isolates: their significance for antibiotic treatment. | enriched broth medium is routinely used as a supplement for agar plate culture of cerebrospinal fluid (csf). to assess the clinical utility of broth cultures, 151 consecutive csf bacterial and fungal isolates obtained from 91 patients were retrospectively reviewed for the effect of results on treatment. treatment decisions associated with individual csf specimens for which isolates were recovered from thioglycollate broth only were compared with the treatment decisions associated with csf specim ... | 1997 | 9260764 |
| proteus syndrome. | this female asian (malay) baby had clinical features of proteus syndrome. she had a large right facial lipolymphangioma with hyperpigmentation of the overlying skin. there was a smaller lymphangioma over the left side of her neck with excess nuchal folds, macrodactyly and bilateral talipes equinovarus. despite the extensive hemifacial swelling, there was no evidence of upper respiratory tract obstruction. generalized seizures developed on the sixth day of life which were controlled with phenobar ... | 1997 | 9259308 |
| the cost effective medical treatment of suppurative otitis media in a nigerian environment. | the microbes isolated from the cultures taken from the discharging ears of 63 patients with otitis media treated in a hospital in warri in 1991 were analysed. pseudomonas, streptococcus, staphylococcus and proteus were the major organisms isolated in order of their prevalence. all show highest sensitivity to gentamycin. the author found 24 aural preparations in the mims of 1992 and checked the availability and cost of these in the nigeria environment and came out with the possible cost effective ... | 1997 | 9257542 |
| mechanisms of action of bactericidal/permeability-increasing protein bpi on reconstituted outer membranes of gram-negative bacteria. | the mechanisms of interaction of the recombinant n-terminal portion of bactericidal/permeability-increasing protein, rbpi21, with various planar asymmetric and symmetric bilayer membranes, including the lipid matrix of the outer membrane of gram-negative bacteria, were investigated via electrical measurements. for the lipopolysaccharide (lps) leaflet of the outer membrane, isolated deep rough mutant lps of escherichia coli strain f515 (f515 lps) and proteus mirabilis strain r45 (r45 lps) were us ... | 1997 | 9254630 |
| inhibitor-resistant beta-lactamases. | 1997 | 9249197 | |
| bartonellosis and human immunodeficiency disease (aids): l-forms as persisters, activating factors, and mechanism of disease. | bartonella, genus proteus, can cause immunodepressive disease. the organisms, in parasitized red blood cells, may invade the brain and every other system and space in the human body. bartonella henselae is proposed to have a role in the pathogenesis of acquired immunodeficiency syndrome (aids) encephalopathy. bartonella bacilliformis produces two known toxins that can induce spasm and angiomatosis, respectively, and manifest as diseases associated with symptomatic aids. the skin lesions of barto ... | 1997 | 9247895 |
| interactions of an antimicrobial peptide, magainin 2, with outer and inner membranes of gram-negative bacteria. | magainin peptides, isolated from xenopus skin, have broad spectra of antimicrobial activity and low toxicities to normal eukaryotic cells, thus being good candidates for therapeutic agents. the mechanism of action is considered to be the permeabilization of bacterial membranes. a number of studies using lipid vesicles have elucidated its molecular detail. however, their interactions with bacteria are not yet well understood. in this paper, we synthesized several magainin analogs with different c ... | 1997 | 9247173 |
| antagonistic activity of lactobacillus acidophilus fermented milk against different pathogenic bacteria. | lactobacillus acidophilus strains tested showed inhibitory activity towards salmonella typhi, staphylococcus aureus, escherichia coli, proteus vulgaris and yersinia enterocolitica. however, wide variations in the activity of the strains were observed. antagonistic activity of the strains exhibited high heat stability (120 min at 92 degrees c, 15 min at 121 degrees c) but it was markedly influenced by changes in ph. effect of kind of milk on antagonistic activity was variable, whereas skimming ha ... | 1996 | 9246920 |
| mini-mu insertions in the tetracycline resistance determinant from proteus mirabilis. | the inducible tetracycline resistance determinant isolated from proteus mirabilis cloned into the plasmid pacyc177 was mutagenized by insertion of a mini-mu-lac phage in order to define the regions in the cloned sequences encoding the structural and regulatory proteins. three different types of mutants were obtained: one lost the resistance phenotype and became lac+; another expressed the resistance at lower levels and constitutively; the third was still dependent on induction but showed a lower ... | 1997 | 9246234 |
| [microbiological contamination of fish in the delta of volga]. | examining 213 bacteriological samples from 57 pike perch has revealed the high contamination of the fish with enterobacteria in the branchia and intestine, which spread into the viscera and tissues. in the kidney there prevalent escherichia coli (27.3%). enterobacteria (20.), and edvardsiella (16.6). the liver most commonly displayed yeasts and fungi (18.2%). citrobacteria (14.3). acinobacteria (14.3%). many microbial strains had proteolytic and hemolytic activities and a considerable resistance ... | 1997 | 9244782 |
| a nonswarming mutant of proteus mirabilis lacks the lrp global transcriptional regulator. | proteus swarming is the rapid cyclical population migration across surfaces by elongated cells that hyperexpress flagellar and virulence genes. the mini-tn5 transposon mutant mns2 was isolated as a tight nonswarming mutant that did not elongate or upregulate flagellar and hemolysin genes. individual cell motility was retained but was reduced. the transposon had inserted in the gene encoding the global transcriptional regulator lrp (leucine-responsive regulatory protein), expression of which was ... | 1997 | 9244260 |
| thyroid hormone receptors in perennibranchiate amphibians. | thyroid hormone has long been known to induce metamorphosis in amphibians. the understanding of the molecular steps controlling the completion of metamorphosis has nevertheless been hampered by the complexity of this event. the comparison of organisms in which metamorphosis does or does not occur, may provide clues into the molecular cascade that control it. up to now the available data suggest that perennibranchiate amphibians retain their larval characters mainly because their tissues do not r ... | 1997 | 9240572 |
| which indwelling urethral catheters resist encrustation by proteus mirabilis biofilms? | to test the resistance of currently available types of indwelling urethral catheters to blockage by encrustation with mineralized proteus mirabilis biofilms. | 1997 | 9240181 |
| eikenella corrodens-caused botryomycosis-type pneumonia in a barbary ape (macaca sylvanus). | an 18-year-old female barbary ape in a safari park died from a mixed bacterial infection. staphylococus aureus was isolated from a purulent necrotic mastitis and from a chronic purulent granulomatous sialoadenitis of the sublingual glands, eikenella corrodens from a botryomycosis-type pneumonia. as judged by histopathology, mixed infection of s. aureus and e. corrodens was present in the sialoadenitis, and e. corrodens botryomycosis-type bacterial colonies were also present in the pancreatic par ... | 1997 | 9236863 |
| epr investigation of compound i in proteus mirabilis and bovine liver catalases: formation of porphyrin and tyrosyl radical intermediates. | compound i of proteus mirabilis and bovine liver catalases (pmc and blc, respectively) were studied combining epr spectroscopy and the rapid-mix freeze-quench techniques. both enzymes, when treated with peroxyacetic acid, form a catalytic intermediate which consists of an oxoferryl porphyrin pi-cation radical. in pmc this intermediate is semistable, and an unexpected reversible equilibrium under ph influence takes place between two forms of compound i with different coupling between the oxoferry ... | 1997 | 9235978 |
| [clinical and bacteriological features of intestinal dysbacteriosis in patients with pulmonary tuberculosis]. | in patients with pulmonary tuberculosis, intestinal dysbacteriosis has clinical and bacteriological features characterized by the short-age of bifidobacteria (in 88-92%), by changes in the composition of escherichia coli strains (in 38-42%), by the presence of candida (in 58-62%), proteus in half the patients, and by associated dysbacteriosis. most (75%) patients show stage iii-iv intestinal dysbiosis formed after 3-4 months of continuous chemotherapy. there is a direct relationship between the ... | 1997 | 9235578 |
| the structure of the capsular polysaccharide from a swarming strain of pathogenic proteus vulgaris. | the structure was determined for the capsular polysaccharide (cps) isolated from a swarming strain of proteus vulgaris, cp2-96, which was obtained from the spleen of an infected mouse. the cps was extracted from the cell pellet by hot water, precipitated with ethanol, and further purified by gel-permeation chromatography. the structure was established by glycosyl composition and linkage analyses, and by nmr spectroscopy. the sequence of the glycosyl residues was determined by a noesy experiment. ... | 1997 | 9232841 |
| multicenter laboratory evaluation of the biomérieux vitek antimicrobial susceptibility testing system with 11 antimicrobial agents versus members of the family enterobacteriaceae and pseudomonas aeruginosa. | a four-center study in which a total of 1,082 recent clinical isolates of members of the family enterobacteriaceae and pseudomonas aeruginosa were examined versus 11 antimicrobial agents with the biomérieux vitek susceptibility test system (hazelwood, mo.) and the gns-f6 card was conducted. in addition, a challenge set consisting of the same 200 organisms was examined in each of the four participating laboratories. results obtained with the vitek system were compared to mics determined by a stan ... | 1997 | 9230393 |
| multicenter study of the efficacy and safety of oral ciprofloxacin in the treatment of chronic suppurative otitis media in adults. the french study group. | chronic suppurative otitis media is a common and potentially dangerous clinical condition that is difficult to treat because the most common infecting organisms are often resistant to many antibiotics. recently, fluoroquinolones such as ciprofloxacin have provided a new therapeutic possibility, offering a wide range of antibacterial activity and proven concentration in the middle ear. therefore in the current study, we assessed the efficacy and safety of oral ciprofloxacin (500 mg twice daily fo ... | 1997 | 9230329 |
| acute pyelonephritis in a child caused by proteus penneri. | 1997 | 9227829 | |
| in-vitro activity of trovafloxacin against sensitive and resistant aerobic bacteria using the standard microdilution broth method and etest. | a comparison of mics of trovafloxacin (cp-99,219) determined by the standard microdilution broth method versus the etest was performed for multiple strains of gram-positive and gram-negative bacteria. a comparison was also made of the in-vitro activity of trovafloxacin versus ciprofloxacin and ofloxacin. the mic50 and mic90 were determined by both methods for each species tested. the etest resulted in mics one to two dilutions higher than the microdilution broth method. trovafloxacin was the mos ... | 1997 | 9222068 |
| multiple, juxtasutural, cranial hyperostoses and cardiac tumor: a new hamartomatous syndrome? | we report on a japanese girl with multiple cranial hyperostoses and a cardiac tumor, both of which manifested in early childhood. unique juxtasutural lesions characterized the cranial findings, including a chain of almost symmetrical osseous protuberances involving the frontozygomatic and frontoparietal junctions, and discrete bony bumps on the right occipitoparietal junction and left temporo-occipital junction. these lesions histologically consisted of thickened mature bone intervened with spar ... | 1997 | 9217216 |
| [proteus syndrome: a case report]. | the proteus-syndrome is a recently described congenital hamartomatosis consisting of numerous clinical features of great variety. mainly affected are the musculo-skeletal system, primarily by hemihypertrophy, macrodactyly and exostoses, and the skin and the subcutaneous tissue, primarily by pigmented naevi and subcutaneous tumors. the differential diagnosis includes other malformation syndromes, e. g. klippel-trenaunay-weber syndrome and other hamartomatosis. surgical intervention and treatment ... | 1997 | 9214178 |
| reservoirs of nosocomial pathogens in neonatal intensive care unit. | a total of 256 swabs taken from different areas of neonatal intensive care units (icu) in kcg hospital and amc hospital, bangalore were bacteriologically investigated for prevalence, source and spread of nosocomial bacteria. culture studies revealed growth in 217 (84.8%) swab samples indicating considerable contamination of different areas of the units and sources of infection. klebsiella pneumoniae (27.3%) was the predominant organism followed by esch coli (16.8%), staph aureus (11.7%), staph e ... | 1997 | 9212573 |
| an in-vitro study of smear layer removal and microbial leakage along root-canal fillings. | the objective of this study was to determine the effect of removal of the smear layer on canal obturation as measured by penetration of bacteria from a coronal direction. fifty-four extracted human teeth were decoronated and instrumented in a uniform manner. following instrumentation the root canals of 20 teeth were rinsed with 17% edta and 5.25% naocl to remove the smear layer before obturation. a second group of 20 teeth were flushed with naocl alone. the teeth of both groups were obturated wi ... | 1996 | 9206432 |
| potentiating effect of edta-tris on the activity of antibiotics against resistant bacteria associated with otitis, dermatitis and cystitis. | possible synergistic effects of the combination of edta-tromethamine (edta-tris) and three antimicrobial agents (cephaloridine, kanendomycin and enrofloxacin) against resistant gram-positive and gram-negative bacteria are reported. bacteria were isolated from eight cases of chronic otitis externa, five cases of chronic dermatitis and four cases of recurrent cystitis in dogs which had previously been treated with one of the three antibiotics without success. animals exposed to edta-tromethamine p ... | 1997 | 9200113 |
| [labyrinthine complications of chronic cholesteatoma in middle ear otitis]. | account clinic and therapeutic of one case, seen and treated by the a., of labyrinthine complication (labyrinthine fistula) secondary from an otic cholesteatoma encroaching the mastoid process. its medicosurgical importance is emphasized. | 1997 | 9199105 |
| [federal investigations on the distribution and in vitro resistance of udder pathogenic bacteria in the milk of cows with subclinical mastitis]. | 1644 quarter milk samples of 948 dairy cows with subclinical mastitis, collected from 63 veterinary practices all over germany origined by 262 livestocks with problems in udder health were examined semiquantitatively by "aulendorfer mastitistest" for cell count and additionally bacteriologically. potentially udder pathogenic bacteria were tested for in vitro-sensitivity to penicillin g, ampicillin, oxacillin, cefacetril, tylosin, neomycin, gentamicin, polymyxin b and enrofloxacin. 24.5% of all t ... | 1997 | 9198960 |
| iron-regulated haemolysin gene from edwardsiella tarda. | we have cloned and sequenced the haemolysin gene locus from edwardsiella tarda (eth). this region encoded two open reading frames, designated etha and ethb. etha is the haemolysin gene consisting of 4782bp encoding a product of 165.3 kda and ethb is an activation/secretion protein gene of 1677bp that encodes a product of 61.9 kda. there were two putative ferric uptake regulator (fur) binding sites on the 5' upstream region of the ethb gene overlapping the promoter region and ribosome-binding sit ... | 1997 | 9194711 |
| antibody responses to gut bacteria in ankylosing spondylitis, rheumatoid arthritis, crohn's disease and ulcerative colitis. | specific immunoreactive anti-klebsiella antibodies are found in patients with ankylosing spondylitis (as), a significant proportion of whom have occult inflammatory bowel disease. molecular mimicry between klebsiella or other bacterial antigens and hla-b27 has been suggested in the pathogenesis of as. the specificity of increased immunoreactivity against klebsiella remains to be assessed against the abundant anaerobic bacterial flora, present either in healthy controls or in patients with ulcera ... | 1997 | 9194209 |
| susceptibility pattern of uropathogenic gram negative bacilli to antimicrobial chemotherapeutic agents in a national hospital in dar es salaam. | in a period of two months, 232 consecutive urinary tract pathogens were isolated from hospitalised and non-hospitalised patients. among the isolates, 200 (86.2%) were gram negative bacilli, including e. coli 109 (54.5%), klebsiella species, 44 (22.5%), enterobacter species 19 (9.5%), proteus species 18 (9%), morganella morganii 9 (4.5%) and salmonella typhimurium, one (0.5%). antimicrobial susceptibility testing to amoxycillin/clavulanic acid, nitrofurantoin, gentamicin and cefuroxime was perfor ... | 1997 | 9185413 |
| [changes in the microbial picture during the production of poultry salami]. | changes in microbiological parameters during the production of fine poultry salami was monitored in a private poultry-processing plant in kosice within a period of six months. this product consists of meat paste or mechanically deboned poultry meat (mdpm), which is produced with the help of a protecon mpb 30 e deboning machine, pork trimmings, water (in the form of crushed ice), salt, spices, and garlic paste. the main raw food material (hand-boned meat and skin from poultry carcasses intended f ... | 1997 | 9182395 |
| tazobactam-piperacillin compared with sulbactam-ampicillin, clavulanic acid-ticarcillin, sulbactam-cefoperazone, and piperacillin for activity against beta-lactamase-producing bacteria isolated from patients with complicated urinary tract infections. | the antibacterial activity of tazobactam-piperacillin was compared with that of sulbactam-ampicillin, clavulanic acid-ticarcillin, sulbactam-cefoperazone and piperacillin against beta-lactamase-producing bacteria isolated from patients with complicated urinary tract infections. tazobactam-piperacillin showed a broad antibacterial spectrum against gram-negative and gram-positive bacteria. the minimum inhibitory concentrations (mic90) of tazobactam-piperacillin were 6.25 micrograms/ml against esch ... | 1997 | 9176745 |
| the construction and application of a lux-based nitrate biosensor. | a plasmid-borne transcriptional fusion between the escherichia coli nitrate reductase (narg) promoter and the photorhabdus luminescens lux operon provides e. coli with a highly bioluminescent phenotype in the presence of nitrate. this e. coli biosensor can detect nitrate to a level of 5 x 10(-5) mol l-1 (0.3 ppm), levels relevant to those levels encountered in brewing water. since induction of the narg promoter requires narl, the plasmid-based sensor can also be used to interrogate enteric bacte ... | 1997 | 9172442 |
| pril-ampicillin-dextrin-ethanol agar for the isolation and quantification of aeromonas spp. from polluted environmental waters. | several selective media were evaluated for their suitability for the isolation and quantification of mesophilic aeromonas species from naturally polluted samples. satisfactory recoveries were obtained with most of them but only when densities of background microflora were low. when analysed samples were from highly polluted waters, results were inconsistent because they did not give quantitative recovery of mesophilic aeromonads or they did not permit ready differentiation of aeromonas species f ... | 1997 | 9172397 |
| thyroid hormone receptor genes of neotenic amphibians. | since thyroid hormones play a pivotal role in amphibian metamorphosis we used pcr to amplify dna fragments corresponding to a portion of the ligand-binding domain of the thyroid hormone receptor (tr) genes in several neotenic amphibians: the obligatory neotenic members of the family proteidea the mudpuppy necturus maculosus and proteus anguinus as well as two members of the facultative neotenic ambystoma genus: the axolotl ambystoma mexicanum and the tiger salamander ambystoma tigrinum. in addit ... | 1997 | 9169551 |
| the effect of granulocyte colony-stimulating factor (g-csf) on bacterial translocation in the splenectomized rat. | we created a study group in order to investigate the effects of splenectomy and filgrastim. filgrastim is an immunomodulator granulocyte colony-stimulating factor (g-csf), that affects bacterial translocation. | 1997 | 9164511 |
| d-lysine production from l-lysine by successive chemical racemization and microbial asymmetric degradation. | in order to develop a practical process for d-lysine production from l-lysine, successive chemical racemization and microbial asymmetric degradation were investigated. the racemization of l-lysine proceeded quantitatively at elevated temperatures. a sample of 1000 strains of bacteria, fungi, yeast and actinomyces were screened for the ability to degrade l-lysine asymmetrically. microorganisms belonging to the achromobacter, agrobacterium, candida, comamonas, flavobacterium, proteus, providencia, ... | 1997 | 9163947 |
| the role of o-acetylation in the metabolism of peptidoglycan in providencia stuartii. | the gentamicin 2'-n-acetyltransferase [ec 2.3.1.59; aac(2')-ia] of providencia stuartii was shown to contribute to the o-acetylation of peptidoglycan and mutants that either under- or overexpress the aac(2')-ia gene was characterized phenotypically to possess either lower or higher levels of peptidoglycan o-acetylation, respectively, compared to the wild-type. these mutants were subjected to scanning electron microscopy. p. stuartii pr100, with 42-44% peptidoglycan o-acetylation compared to 54% ... | 1996 | 9158736 |
| spontaneous drainage of an infratentorial extradural empyema: case report. | we describe a 21-year-old male who presented with spontaneous drainage of an infratentorial extradural empyema secondary to mastoiditis. following eradication of the primary source, the patient was commenced on high dose intravenous antibiotics. serial computed tomography (ct) revealed complete resolution of the pus collection. antibiotic therapy was successful and no neurosurgical intervention was necessary. | 1997 | 9156024 |
| [maggot therapy for gangrene and osteomyelitis]. | 5 patients with diabetic-foot were treated by maggot therapy. the most serious case was in a 75-year-old man who had gangrene and osteomyelitis of the right foot. proteus mirabilis, enterococcus sp., providencia stuartii and staphylococcus spec. (coagulase positive) were isolated from lesions which did not respond to antibiotic therapy. the patient had twice refused amputation but agreed to maggot therapy. larvae of the sheep blowfly phoenicia (lucilia) sericata were used for twice-weekly treatm ... | 1997 | 9153871 |
| the ability of a proteus mirabilis strain to invade the bloodstream is independent of its proticine production/proticine sensitivity type. | proteus mirabilis strains frequently infect the upper urinary tract and some progress to invade the bloodstream. in an attempt to determine if these were features only of particular strains of p. mirabilis, isolates from the blood of 38 unrelated patients, many of whom had an underlying urinary tract infection, and eight from different sources and sites within the upper urinary tract, were analysed by proticine production/proticine sensitivity (p/s) typing. twenty-six different p/s types were fo ... | 1997 | 9152037 |
| [urinary tract infections: risk factors and therapeutic trends]. | urinary tract infections (utis) are very common in medical practice. women have a high prevalence of utis, approximately 50 times higher than men. a large proportion of this prevalence is probably caused by anatomic and physical factors chemical analysis of urine composition, examination of the urinary sediment and the bacterial colony counts are of great value for diagnosis and therapy. the patients may be benefit from antibiotic doses. in addition to trimethoprimsulfamethoxazole (tmp/smz), amo ... | 1997 | 9148368 |
| antibacterial activity of bms-180680, a new catechol-containing monobactam. | the in vitro activities of a new catechol-containing monobactam, bms-180680 (sq 84,100), were compared to those of aztreonam, ceftazidime, imipenem, piperacillin-tazobactam, ciprofloxacin, amikacin, and trimethoprim-sulfamethoxazole. bms-180680 was often the most active compound against many species of the family enterobacteriaceae, with mics at which 90% of the isolates were inhibited (mic90s) of < or = 0.5 microg/ml for escherichia coli, klebsiella spp., citrobacter diversus, enterobacter aero ... | 1997 | 9145861 |
| etest evaluation of enterobacteriaceae with reduced susceptibility to ciprofloxacin. | 1997 | 9145832 | |
| preoperative treatment of children with chronic suppurative otitis media. | 259 patients with chronic suppurative otitis media were bacteriologically assessed. 12 bacterial strains were isolated in 196 of the cases. the most common species present was staphylococcus - 77 (39%) cases, followed by proteus vulgaris isolated in 36 (18%) cases, and pseudomonas aeruginosa in 33 (17%) cases. the flora in 25 cases (18%) yielded two bacterial strains with identical oxygen demands, and a mixture of staphylococcus and streptococcus in 32%. in 60.6% of cases the flora was sensitive ... | 1996 | 9145593 |
| [the validation of empirical emergency antibacterial therapy in acute pyelonephritis]. | as many as 254 patients with acute pyelonephritis were evaluated for the whole range of possible inflammatory process pathogens with the aid of automatic microbiological system [symbol: see text]. urine from the urinary bladder, renal pelves (as directed) was studied as was biopsy specimen of the kidney. analysis of species range of the identified organisms showed escherichia coli, proteus, and mycoplasma, enterobacter, coccal flora and pseudomonads to be in 27.9%, 15.9%, 14.9%, 8.6%, and 5.5% r ... | 1996 | 9138828 |
| [the role of pseudomonas aeruginosa and proteus in the etiology of suppurative surgical infection]. | 1996 | 9138809 | |
| association of gut inflammation with increased serum iga class klebsiella antibody concentrations in patients with axial ankylosing spondylitis (as): implication for different aetiopathogenetic mechanisms for axial and peripheral as? | a role for klebsiella pneumoniae in ankylosing spondylitis (as) has been suggested because faecal carriage of klebsiella and serum klebsiella specific antibodies may be increased in this disease. this study has extended the earlier findings by comparing klebsiella specific serum iga class antibodies with inflammatory changes in the gut. | 1997 | 9135221 |
| non-operative management of a splenic laceration in a patient with the proteus syndrome. | an adult patient with the proteus syndrome sustained a grade iii splenic laceration after falling off a horse. clinical features of this rare disorder include subcutaneous and visceral hamartomatous tumours. the patient also suffered from chronic intravascular coagulation associated with extensive haemangiomatosis (kasabach-merritt syndrome). considering the visceral anomalies and abnormal coagulation, a non-operative approach was preferred despite considerable transfusion requirement, and the p ... | 1997 | 9132186 |
| effect of preventive administration of a nonpathogenic escherichia coli strain on the colonization of the intestine with microbial pathogens in newborn infants. | in a randomized, double-blind study, 27 healthy newborn infants were colonized with the nonpathogenic escherichia coli strain nissle 1917 (e. coli dsm 6601, mutaflor) during the first 5 days of life by daily oral inoculation of 1 ml of a suspension with 10(8) living cells. a second group of 27 newborns, used as controls, received a placebo suspension (1 ml of phosphate-buffered saline) instead. stool samples were taken on days 1, 2, 3, 5, and 21, and 6 months after birth. all samples were examin ... | 1997 | 9129791 |
| bacteriology of ethmoid sinus in chronic sinusitis. | this study was designed to study the bacteriology of the ethmoid sinus in chronic sinusitis with a new sampling method to decrease nasal contamination. the anterior nose was disinfected with povidone-iodine solution. then, the sample of ethmoid sinus was obtained with a cotton-tip stick through a cannula that was put into the ethmoid cavity after removing ethmoid bulla. thirty-nine patients of chronic sinusitis were included with a total of 69 specimens. fifty-eight bacterial isolates were recov ... | 1997 | 9129755 |
| [a case of bladder calculus due to a ruptured balloon fragment of a foley catheter]. | i report a case of bladder calculus developing on a ruptured balloon fragment of the foley catheter as the nidus. a 65-year-old man suffering from cerebral infarction had been managed with an indwelling urethral foley catheter for one month. since the balloon could no longer be deflated, the foley catheter was removed after rupturing the balloon by overinflation. four months later, a 3-cm bladder calculus was detected on the plain abdominal roentgenogram. transurethral cystolitholapaxy revealed ... | 1997 | 9127760 |
| [experimental bladder stone production by human uropathogenic bacteria]. | to investigate the formation of infection stones by 5 bacterial species isolated from patients with urinary calculi, such as proteus mirabilis, staphylococcus saprophyticus, pseudomonas aeruginosa, klebsiella pneumoniae and escherichia coli, a zinc disc was implanted and 10(6) bacteria were surgically inoculated at the same time into the bladder of male wistar rats (10 rats per group). urinary ph was measured on days 1, 3, 5 and 7. the rats were killed on day 7 and the bladder examined for the f ... | 1997 | 9127756 |
| detection and analysis of the growth characteristics of proteus vulgaris with a bulk acoustic wave ammonia sensor. | a bulk acoustic wave ammonia sensor to monitor the growth of proteus vulgaris is described. the corresponding bacterial growth response equation was derived based on the basic growth characteristics of p. vulgaris, and was verified experimentally. the application of the equation to the estimation of kinetic parameters (such as the asymtote, the maximum specific growth rate and the lag time) was accomplished successfully. the generation time is 33.3 min at 37 degrees c. the influence of temperatu ... | 1997 | 9124699 |
| proteus mirabilis mannose-resistant, proteus-like fimbriae: mrpg is located at the fimbrial tip and is required for fimbrial assembly. | the mannose-resistant, proteus-like (mr/p) fimbria, responsible for mannose-resistant hemagglutination, is a virulence factor for uropathogenic proteus mirabilis. based on known fimbrial gene organization, we postulated that mrpg, a putative minor subunit of the mr/p fimbria, functions as an adhesin responsible for hemagglutination, while mrpa serves as the major structural subunit for the filamentous structure. to test this hypothesis, an mrpg mutant was constructed by allelic-exchange mutagene ... | 1997 | 9119470 |
| structural and serological studies of the o-antigen of the bacterium proteus mirabilis oxk (serogroup o3) used in the weil-felix test. | sugar analysis and 1h- and 13c-nmr spectroscopic studies showed that various strains of proteus mirabilis oxk used as antigens in the weil-felix test for serodiagnosis of rickettsiosis (scrub typhus) produce lipopolysaccharides (lpss) with the same o-specific polysaccharide chain having the following structure: [formula: see text] where glca and gala are glucuronic and galacturonic acids, respectively. this polysaccharide which defines the o3 specificity of proteus and has been found earlier in ... | 1997 | 9113725 |
| structural and serological studies of the o-antigen of the bacterium proteus vulgaris ox2 (serogroup o2) used in the weil-felix test. | based on monosaccharide analysis and 1h- and 13c-nmr spectroscopy, the following structure of the o-specific polysaccharide chain of proteus vulgaris ox2 lipopolysaccharide (lps), which defines the o2 specificity of proteus, was established: [formula: see text] where l-quinac is n-acetyl-l-quinovosamine (2-acetamido-2,6-dideoxy-l-glucose). various strains of p. vulgaris ox2 used in the weil-felix test for serodiagnosis of rickettsiosis (spotted fevers, except for rocky mountain spotted fever) we ... | 1997 | 9113724 |
| spinal canal compromise in proteus syndrome: case report and review of the literature. | a 24-year-old vietnamese man with proteus syndrome presented to the emergency department with progressive paraplegia and underwent urgent operative decompression of an angiolipomatous mass compressing the spinal cord. the associated spinal deformity and difficulty in imaging the mass, together with the paucity of discussion regarding the spinal cord sequelae of this rare syndrome in the literature, made this an extremely challenging case. spinal cord compromise in patients with proteus syndrome ... | 1997 | 9113294 |
| prevalence of pathogenic role of morganella-proteus providencia-group of bacteria in human faeces. | speculation in literature that organisms of the morganella-proteus-providencia (mpp) group can cause diarrhoea led to the present studies which investigated their occurrence and pathogenic role in human faeces. faecal specimens were collected from a total of 307 subjects, 80 of which were from diarrhoea cases and 277 from healthy controls. ninety-two species of the mpp (24/80 diarrhoea and 68/277 control) were isolated from 30% of all subjects. none of the species of the mpp group was significan ... | 1996 | 9110049 |
| potential virulence factors of proteus bacilli. | the object of this review is the genus proteus, which contains bacteria considered now to belong to the opportunistic pathogens. widely distributed in nature (in soil, water, and sewage), proteus species play a significant ecological role. when present in the niches of higher macroorganisms, these species are able to evoke pathological events in different regions of the human body. the invaders (proteus mirabilis, p. vulgaris, and p. penneri) have numerous factors including fimbriae, flagella, o ... | 1997 | 9106365 |
| antimicrobial susceptibilities of blood culture isolates obtained before and after the introduction of ciprofloxacin. | the in-vitro susceptibilities of 1658 blood culture isolates to ciprofloxacin and 13 other antimicrobial agents were determined, and compared with the results for isolates obtained before and after the availability of ciprofloxacin in 1989. only six (0.6%) of 995 enterobacteriaceae were resistant to the fluoroquinolones tested; all of the ciprofloxacin-resistant strains were isolated after 1989 (p = 0.04). no significant increase in ciprofloxacin resistance was found in pseudomonas aeruginosa or ... | 1997 | 9096194 |
| a national collaborative study of the in vitro activity of oral cephalosporins and loracarbef (ly 163892). australian group for the study of antimicrobial resistance (agar). | a national collaborative study involving the laboratories of 17 australian hospitals examined the in vitro activity of loracarbef, cefaclor, cephalexin, amoxycillin and amoxycillin/clavulanate against 2661 recently isolated common bacterial pathogens. loracarbef was the most active agent against escherichia coli (mic90 = 1 mg/l) and had activity comparable to other agents against klebsiella pneumoniae and proteus mirabilis. like the oral cephalosporins, it had no activity against species of ente ... | 1997 | 9094185 |
| [alkaline encrusted cystitis secondary to proteus mirabilis infection in a hiv infected patient]. | we described for the first time an alkaline encrusting cystitis in a patient with the acquired immune deficiency. this is an entity characterized by severe dysuria and long standing urinary frequency, alkaline urine and radiographically visible calcification of the urinary bladder. it has been observed in patients with permanent foley catheter, persistent infections due to microorganisms with urolytic activity, urinary bilharziosis or tuberculosis, or malignant bladder tumors. none of these enti ... | 1997 | 9091033 |
| structure of the o-specific polysaccharide of proteus penneri strain 25 containing n-(l-alanyl) and multiple o-acetyl groups in a tetrasaccharide repeating unit. | based on sugar and methylation analyses, o-deacetylation, smith degradation, and 1h and 13c nmr spectroscopy, including 2d cosy, 1h-detected 1h, 13c heteronuclear single-quantum coherence (hsqc), and 1h-detected 1h, 13c heteronuclear multiple-bond connectivity (hmbc) experiments, the following structure of the o-specific polysaccharide of proteus penneri strain 25 was established: [formula: see text] where d-glcn(l-ala) is 2-(l-alanylamido)-2-deoxy-d-glucose. | 1997 | 9090817 |
| false aneurysm of the axillary artery caused by graft infection: report of a case. | a 77-year-old man presented to our hospital following the sudden onset of left subclavicular swelling, 8 years after undergoing subtotal removal of an axillofemoral bypass. computed tomography revealed a rupture of the left axillary artery with the formation of a false aneurysm. emergency surgery was performed in the form of an extraanatomic, axillo-axillary bypass with resection of the false aneurysm. prior to removal of the false aneurysm, the subclavian artery was ligated via a left thoracoto ... | 1997 | 9086560 |
| acquired tracheomalacia--a case report. | tracheomalacia is a condition characterized by weakness of the tracheal walls and supporting cartilage. it may be congenital, occurring in association with other lesions, such as vascular ring, tracheoesophageal fistula, dyschondroplasia, congenital cysts or tumors, or it may be acquired, most commonly as a result of protracted endotracheal intubation and less often of irradiation, trauma or neoplasm. we present a case of acquired tracheomalacia in a 15-year-old boy, a victim of proteus syndrome ... | 1996 | 9084553 |
| two distinct chondroitin sulfate abc lyases. an endoeliminase yielding tetrasaccharides and an exoeliminase preferentially acting on oligosaccharides. | crude enzyme obtained from chondroitin sulfate-induced proteus vulgaris nctc 4636 has been fractionated into 1) an endoeliminase capable of depolymerizing chondroitin sulfate and related polysaccharides to produce, as end products, a mixture of delta4-unsaturated tetra- and disaccharides and 2) an exoeliminase preferentially acting on chondroitin sulfate tetra- and hexasaccharides to yield the respective disaccharides. isolation of the two enzymes was achieved by a simple two-step procedure: ext ... | 1997 | 9083041 |
| a simple artificial urine for the growth of urinary pathogens. | a simple artificial urine medium (aum) has been developed which provides conditions similar to that found in human urine. aum solidified with agar enabled the recovery of a wide range of urease-positive and -negative urinary pathogens. liquid aum supported growth at concentrations of up to 10(8) cfu ml-1, as found in normal urine. reproducible, steady-state growth also occurred over many generations in continuous culture. aum was capable of forming crystals and encrustations resembling those fou ... | 1997 | 9080700 |
| the rtn gene of proteus vulgaris is actually from escherichia coli. | the rtn gene, identified as coming from proteus vulgaris atcc 13315, is present in escherichia coli k-12, and over a 440-bp region of rtn is identical to the published proteus sequence, with the exception of a single g insertion. it was not possible to verify the presence of rtn in p. vulgaris. | 1997 | 9079933 |
| in vivo phase variation of mr/p fimbrial gene expression in proteus mirabilis infecting the urinary tract. | proteus mirabilis, associated with complicated urinary tract infection, expresses mannose-resistant/proteus-like (mr/p) fimbriae. expression of these surface structures, which mediate haemagglutination and have a demonstrated role in virulence, undergoes phase variation. by dna sequence analysis, a 252 bp invertible element was found in the intergenic region between mrpl, the putative site-specific recombinase gene, and mrpa, the primary structural subunit gene. the invertible segment is flanked ... | 1997 | 9076737 |
| [serologic cross reactions between strains of proteus mirabilis belonging to serogroup o43 i o10]. | in this work an epitope has been determined on lipopolysaccharide of proteus mirabilis o43. this epitope is recognized by specific antibodies. | 1996 | 9072767 |
| [serologic studies of cross reactions between strains of proteus mirabilis oxk (pro 10/52) and proteus mirabilis s1959]. | strong cross-reactions are described between proteus mirabilis strains having the same structures of repeating units of their o-specific polysaccharides. these strains are used in routine diagnosis of rickettsiae. | 1996 | 9072766 |
| [proteus lipopolysaccharides]. | 1996 | 9072758 | |
| new combination for the therapy of canine otitis externa. i. microbiology of otitis externa. | in order to compound a new drug combination against canine otitis externa (oe), 515 dogs affected with oe were subjected to physical examination and microbiological analysis of their ear exudates. oe was erythematous-ceruminous in 83 per cent and suppurative in 17 per cent of the patient material. erythematous-ceruminous inflammations were characterised by severe pruritus and accumulation of brownish, greasy cerumen in the auditory canal. the yeast malassezia pachydermatis was isolated from the ... | 1997 | 9065882 |
| [aids manifestations in otorhinolaryngology]. | the otorhinolaryngological signs of aids are reviewed (both analysis and synthesis) following the chronological order of the literature. the earliest clinical pictures, their frequency and time of onset, are described by the authors studied. in 1986 the ent signs of this disease were well known, and in our region the same multiple, polyfacetic aspects are seen. personal experience of this is described, emphasizing how seldom the diagnosis has been made, except early on. usually the cases seen an ... | 1996 | 9064187 |
| tuning of electroreceptors in the blind cave salamander, proteus anguinus l. | the blind cave salamander's (proteus anguinus l.) sensitivity for electrical fields was determined by recordings from afferent fibers coming from ampullary electroreceptors. the animals were stimulated with single rectangular or continuous sinusoidal electrical signals passed through the cave water in the experimental tank. best threshold sensitivities of the fibers for both kinds of signals were in the same range (0.1-0.5 mv/cm = 30-150 na/cm2). the fibers showed phase-locked responses to rostr ... | 1997 | 9063591 |
| behavioral sensitivity of the european blind cave salamander, proteus anguinus, and a pyrenean newt, euproctus asper, to electrical fields in water. | in the urodeles proteus anguinus and euproctus asper, thresholds of an overt avoidance response to weak electrical field stimuli (continuous sine-waves) were measured as a function of frequency. thresholds down to 0.1 mv/cm (30 na/cm2) were found in p. anguinus and 2 mv/cm (600 na/cm2) in e. asper at 'best frequencies' (b.f.) of 20-30 hz, but sensitivity covered a total frequency range of below 0.1 hz to 1-2 khz, with up to 70 db higher thresholds. average thresholds of 1 mv/cm in p. anguinus an ... | 1997 | 9063590 |
| the responses of lucilia cuprina to odours from sheep, offal and bacterial cultures. | the responses of gravid female lucilia cuprina to odours from sheep urine, faeces and gut mucus, and to odours from liver/sodium sulphide mixtures was tested using a bioassay which measured the movement and probing response of walking flies. the same bioassay was used to test the response to odours from cultures of bacteria isolated from liver/sodium sulphide and liver/water mixtures. a significant movement towards odours from faeces, gut mucus and urine was observed. odours from cultures of the ... | 1997 | 9061678 |
| the effect of the bacterial product, succinic acid, on neutrophil bactericidal activity. | we investigated the effect of succinic acid on neutrophil bactericidal activity in a model of intra-abdominal abscess induced in mice by the peritoneal inoculation of 5 x 10(6) cfu ml-1 e. coli and 5 x 10(8) cfu ml-1 b. fragilis plus 1 mg of bran as faecal fibre analogue. the mean ph of the induced abscesses at week 1 was 6.7, higher than the ph associated with succinic acid inhibitory activity. we therefore determined the effect of succinic acid (0-100 mm) at ph 6.7 on the bactericidal activity ... | 1997 | 9061353 |
| increased soluble icam-1 concentration and impaired delayed-type hypersensitivity skin tests in patients with chronic liver disease. | soluble icam-1 may act as an antagonist of the membrane bound form, which is essential for the adhesion of leucocytes to endothelial cells. the aim of this study was to investigate whether the presence of high concentrations of soluble icam-1 are related to the impairment of delayed-type hypersensitivity reactions. | 1997 | 9059357 |
| morganella morganii postoperative endophthalmitis. | 1997 | 9059257 | |
| an homogeneous fluorescence polymerase chain reaction assay to identify salmonella. | we have developed a semiquantitative homogeneous fluorescence assay that combines polymerase chain reaction (pcr) amplification with direct fluorescence detection (hf-pcr). the assay eliminates the need to perform gel electrophoresis on test samples. using a set of salmonella-specific primers, this system was used to verify suspect colonies from culture plates as salmonella. the fluorescence signal is generated by a nucleic acid dye, yo-pro-1, that is included in the amplification reaction. this ... | 1997 | 9056213 |
| antimicrobial action of rabbit leukocyte cap18(106-137). | cap18 is a cationic antimicrobial protein originally isolated from rabbit neutrophils, of which a 32-mer sequence from its c-terminal and (cap18(106-137)) has been found to be the most active. the bactericidal action of this peptide has been characterized by conventional culture techniques and flow cytometry. cultures of escherichia coli nctc10418 were exposed to the mbc (12 microm) of the peptide for up to 60 min and stained with a fluorochrome sensitive to changes in either membrane potential ... | 1997 | 9056004 |