Publications

chromogenic detection of aminoglycoside phosphotransferases.	a coupled chromogenic reaction (based on an agar overlay combining nadh, pyruvate kinase, lactate dehydrogenase, phosphoenolpyruvate, atp, and kanamycin sulfate with thiazolyl blue-phenazine methosulfate for detection of nadh consumption) was optimized for the detection of aminoglycoside phosphotransferases (aphs). when used after analytical isoelectrofocusing of bacterial extracts from aph-producing strains, this method revealed one band in each of two strains with a genetically confirmed aph ( ...	1998	9527764
response of blood platelets to proteus mirabilis lipopolysaccharide.	the effects of the lipopolysaccharide (lps) of proteus mirabilis on the production of thiobarbituric acid reactive substances (tbars) and the generation of superoxide radicals (o2) by pig blood platelets were studied in vitro. the effect of lps on tbars formation in platelets was dependent on the concentration of endotoxin. lps at concentrations above 0.1 microg/10(8) platelets caused the production of tbars concomitant with the generation of superoxide radicals. the responses of platelets to lp ...	1998	9525779
structural and immunochemical studies of two cross-reactive proteus mirabilis o-antigens, o6 and o23, containing beta1-->3-linked 2-acetamido-2-deoxy-d-glucopyranose residues.	a marked serological cross-reactivity was observed by elisa and a precipitation test between anti-proteus mirabilis o23 serum and the lipopolysaccharide as well as the o-specific polysaccharide from the proteus mirabilis strain belonging to serogroup o6. the structures of the o-specific polysaccharides were elucidated using chemical and nmr spectroscopic analyses, and the only common component, 2-acetamido-2-deoxy-beta-d-glucopyranose (beta-d-glcnac), was revealed, which was suggested to be resp ...	1998	9525774
the interaction of phospholipid liposomes with bacteria and their use in the delivery of bactericides.	liposomes have been prepared from dipalmitoylphosphatidylcholine (dppc) incorporating the cationic lipids stearylamine (sa), dimethyldioctadecylammonium bromide (ddab) and dimethylaminoethane carbamoyl cholesterol (dcchol) and the anionic lipids dipalmitoylphosphatidylglycerol (dppg) and phosphatidylinositol (pi). their adsorption to biofilms of skin-associated bacteria (staphylococcus epidermidis and proteus vulgaris) and oral bacteria (streptococcus mutans and sanguis) has been investigated as ...	1997	9524311
[a rare combination of complications of acute necrotizing pancreatitis].		1997	9518132
flies and their bacterial loads in greyhound dog kennels in kansas.	breeders of greyhound dogs traditionally feed racing animals and nursing bitches raw meat, and that meat generally is obtained frozen from commercial renderers. previous studies have shown that the rendered meat is frequently contaminated with enteric bacteria, including salmonella spp., and that during thawing the rendered meat is exposed to filth flies common in dog kennels. nursing greyhound pups tend to experience a high morbidity and mortality from intestinal infections, and we attempted to ...	1998	9516545
interaction of alpha-helical peptides with phospholipid membrane: effects of chain length and hydrophobicity of peptides.	to investigate the interaction of amphiphilic alpha-helical peptides with phospholipid membranes, we synthesized ac-(leu-ala-arg-leu)3-nhch3 (4[3]) and three derivatives, in which the chain length and the size of the hydrophobic region of the peptides were different from each other. these peptides formed an alpha-helical structure in the presence of vesicles. in the membrane-perturbation measurement, only 43 showed a strong membrane-perturbation activity below phase-transition temperature (25 de ...	1998	9516044
bacteriophages show promise as antimicrobial agents.	the emergence of antibiotic-resistant bacteria has prompted interest in alternatives to conventional drugs. one possible option is to use bacteriophages (phage) as antimicrobial agents. we have conducted a literature review of all medline citations from 1966-1996 that dealt with the therapeutic use of phage. there were 27 papers from poland, the soviet union, britain and the u.s.a. the polish and soviets administered phage orally, topically or systemically to treat a wide variety of antibiotic-r ...	1998	9515662
discovery of novel pyridinopolyamines with potent antimicrobial activity: deconvolution of mixtures synthesized by solution-phase combinatorial chemistry.	a 1638-member pyridinopolyamine library, consisting of 13 sublibraries of 126 members prepared by a solution-phase approach, was completely deconvoluted from orthogonally protected intermediates by a combination of iterative and positional scanning procedures. antibacterial assays against streptococcus pyogenes and escherichia coli imp- and a candida albicans yeast specificity assay were employed to follow the activity of sublibraries. screening of the 13 sublibraries, which were prepared by a s ...	1998	9513599
site-directed mutagenesis of the proteus mirabilis glutathione transferase b1-1 g-site.	in order to investigate the roles of near n-terminus tyr, cys, and ser residues in the activity of bacterial glutathione transferase (gstb1-1) site-directed mutagenesis was used to replace the following residues: tyr-4, tyr-5, ser-9, cys-10, ser-11, and ser-13. the results presented here show that, unlike all other alpha, mu, pi, theta and sigma classes of glutathione transferases so far investigated, gstb1-1 does not utilise any tyr, ser or cys residue to activate glutathione. these results als ...	1998	9512342
alice vance ("das bärenweib"): a historical case of nievergelt syndrome.	several malformed individuals were presented at the world exhibition in antwerp in 1894. among them was mrs. alice vance from mount pleasant, texas, with congenital limb defects, and mr. eugen berry, who had asymmetrical, monstrous enlargement and macrodactyly of the feet, i.e., proteus syndrome. after the world exhibition mrs. vance presented herself to the public in castan's panopticon imitating a bear. she became famous under the stage name "das bärenweib" ("the bear-like woman") and was exam ...	1998	9511977
ciprofloxacin versus cefuroxime axetil in the treatment of adult patients with acute bacterial sinusitis.	this study compared the use and efficacy of ciprofloxacin to cefuroxime axetil for adult patients with acute bacterial sinusitis.	1998	9511113
unexpected high prevalence of secondary bacterial infection in patients with mycetoma.		1998	9508332
reduction of carriage of enterohemorrhagic escherichia coli o157:h7 in cattle by inoculation with probiotic bacteria.	bacteria inhibitory to escherichia coli o157:h7 were isolated from cattle and evaluated for their potential for reducing carriage of e. coli o157:h7 in calves. eighteen of 1,200 bacterial isolates from cattle feces and intestinal tissue samples were screened and determined to inhibit the growth of e. coli o157:h7 in vitro. seventeen of the isolates were e. coli and one was proteus mirabilis. none produced shiga toxin. genomic dna fingerprinting by pulsed-field gel electrophoresis revealed 13 dis ...	1998	9508288
expression of the carboxypeptidase t gene from thermoactinomyces vulgaris in stable protoplast type l-forms of proteus mirabilis.	the structural gene of the carboxypeptidase t (cpt) was successfully expressed in cell wall-less l-form cells of proteus mirabilis. the dna sequence encoding the phoa leader peptide was fused with a truncated cpt gene encoding the mature enzyme. the modified gene in a puc-based kanamycin resistance vector under the control of the lac promoter was transformed into l-form cells of p. mirabilis. they were able to produce the recombinant cpt both as a secretory and as a cell-bound insoluble form. th ...	1998	9503606
purification and characterization of a novel glutathione transferase from ochrobactrum anthropi.	glutathione transferase was purified from ochrobactrum anthropi and its n-terminal sequence was determined to be mklyykvgacslaphiilseaglpy. the apparent molecular mass of the protein (24 kda) was determined by sds-polyacrylamide gel electrophoresis analysis. the amino acid sequence obtained showed similarities with known bacterial glutathione transferases in the range of 72-64%. immunoblotting experiments performed with antisera raised against glutathione transferase from o. anthropi did not sho ...	1998	9495016
bacteraemia in the elderly.	the aims of the study were to describe community-acquired and nosocomial bacteraemia in elderly patients and to determine the factors associated with increased mortality in these patients attending a tertiary hospital in singapore. a consecutive series of 191 patients aged more than 60 years of age admitted in 1995 was studied retrospectively. all of them had positive blood culture results obtained from the department of pathology and the case notes were reviewed and entered into a standard clin ...	1997	9494663
[urinary bacteria in elderly patients with urinary incontinence and low levels of daily activity].	the results of bacteria isolated from the urine of inactive elderly patients with urinary incontinence in 1982, 87, 93 and 95 were compared and the chronological changes of urinary bacteria were analyzed. the following results were obtained: (1) the most common bacteria isolated from the patients in 1995 were d-group enterococcus, followed by providencia rettgeri, e. coli, pseudomonas aeruginosa and proteus mirabilis. it is thought that the main cause of the more frequent isolation of enterococc ...	1997	9493467
in vitro activity of fosfomycin trometamol against pathogens from urinary tract infections: a spanish multicenter study.	the in-vitro susceptibilities of a total of 1371 urinary tract pathogens to fosfomycin trometamol were determined. according to the nccls breakpoints, enterobacteriaceae and gram-positive microorganisms were, in general, very sensitive to this antimicrobial. more than 90.0% of the escherichia coli and citrobacter spp. and more than 70.0% of the klebsiella pneumoniae, k. oxytoca, enterobacter spp., proteus mirabilis, staphylococcus aureus, coagulase-negative staphylococci and enterococcus spp. st ...	1997	9491838
bacterial pathogens and their antimicrobial susceptibility in kumasi, ghana.	between january, 1994 and june, 1996 a survey of bacterial isolates from clinical specimens and their antimicrobial susceptibility was performed at the komfo anokye teaching hospital, microbiology department, kumasi, ghana. a total of 11,380 bacterial isolates were cultured from eight different specimens. the sites of origin were wounds 32.2%, urine 28.1%, ear, nose and throat 3.6%, sputum 2.5% and aspirates 2.5%. gram-negative bacteria accounted for 7955 (69.9%) isolates, the main species were ...	1997	9491183
structural properties of lipopolysaccharides from rickettsia typhi and rickettsia prowazekii and their chemical similarity to the lipopolysaccharide from proteus vulgaris ox19 used in the weil-felix test.	the lipopolysaccharides (lpss) isolated from typhus group (tg) rickettsiae rickettsia typhi and rickettsia prowazekii were characterized by chemical analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) followed by silver staining. lpss from two species of tg rickettsiae contained glucose, 3-deoxy-d-manno-octulosonic acid, glucosamine, quinovosamine, phosphate, and fatty acids (beta-hydroxylmyristic acid and heneicosanoic acid) but not heptose. the o-polysaccharides o ...	1998	9488376
transmucosal passage of bacteria across rat intestinal epithelium in the ussing chamber: effect of nutritional factors and bacterial virulence.	transmucosal passage of bacteria across the intestine, the essential and prerequisite step for bacterial translocation, cannot be effectively studied using in vivo models of translocation. we have adapted the ussing chamber into a fresh, sterile organ culture system that can facilitate the study of bacterial-epithelial interactions. intestinal membranes were mounted in the ussing chamber and perfused with a solution rich in putative mucosal micronutrients. the transmembrane potential difference ...	1998	9488257
h2m3wt-restricted, listeria monocytogenes-immune cd8 t cells respond to multiple formylated peptides and to a variety of gram-positive and gram-negative bacteria.	a subset of h2m3wt-restricted, listeria monocytogenes (lm)-immune cd8 effectors recognize antigen-presenting cells (apc) preincubated with heat-killed lm. the responsible product, which we have previously designated heat-killed listeria-associated antigen (haa), is extremely hydrophobic and resistant to proteolytic degradation. despite the protease resistance of haa, we now report that haa-immune clones are uniformly responsive to fmigwii, a formylated oligopeptide derived from the recently desc ...	1998	9488151
expression and secretion of functional miniantibodies mcpc603scfvdhlx in cell-wall-less l-form strains of proteus mirabilis and escherichia coli: a comparison of the synthesis capacities of l-form strains with an e. coli producer strain.	the paper describes the synthesis of the phosphorylcholine-binding miniantibody mcpc603scfvdhl x in cell-wall-less l-form strains of escherichia coli and proteus mirabilis. cells of these strains were transformed with the plasmid pack02sckan, carrying the miniantibody (miniab) coding sequence under the control of the lac promoter. l-form transformants of both species were able to synthesize the functional miniab as an extracellular soluble product. the highest quantities were obtained by p. mira ...	1998	9487710
enhanced jejunal production of antibodies to klebsiella and other enterobacteria in patients with ankylosing spondylitis and rheumatoid arthritis.	to measure gut immunity directly in jejunal fluid in patients with ankylosing spondylitis (as) and rheumatoid arthritis (ra).	1997	9486004
the axial tyrosinate fe3+ ligand in protocatechuate 3,4-dioxygenase influences substrate binding and product release: evidence for new reaction cycle intermediates.	the essential active site fe3+ of protocatechuate 3,4-dioxygenase [3, 4-pcd, subunit structure (alphabetafe3+)12] is bound by axial ligands, tyr447 (147beta) and his462 (162beta), and equatorial ligands, tyr408 (108beta), his460 (160beta), and a solvent oh- (wat827). recent x-ray crystallographic studies have shown that tyr447 is dissociated from the fe3+ in the anaerobic 3,4-pcd complex with protocatechuate (pca) [orville, a. m., lipscomb, j. d., and ohlendorf, d. h. (1997) biochemistry 36, 100 ...	1998	9485360
the role of fluoroquinolones in respiratory tract infections.	quinolones, because of their excellent pharmacokinetic properties, high antimicrobial activity and low incidence of side-effects, have been broadly accepted for the treatment of many infections. because of perceived limitations against streptococcus pneumoniae and methicillin-resistant staphylococcus aureus there has been some reluctance to use them for respiratory tract infections. ciprofloxacin is the drug of choice for malignant external otitis, a disease caused by pseudomonas aeruginosa, and ...	1997	9484874
structure of a neutral o-specific polysaccharide of the bacterium proteus mirabilis o24.	lipopolysaccharide of the bacterium proteus mirabilis o24 was found to have a neutral o-specific polysaccharide chain containing d-galactose, 2-acetamido-2-deoxy-d-glucose, and 2-acetamido-2-deoxy-d-galactose in ratios 1:2:1. on the basis of 1h- and 13c-nmr spectroscopy, including two-dimensional correlation spectroscopy (cosy), h-detected 1h, 13c heteronuclear multiple-quantum coherence (hmqc), and nuclear overhauser effect spectroscopy (noesy), the following structure of the branched tetrasacc ...	1997	9481878
[treatment of lower respiratory tract infections in the elderly].	sixty outpatients at the age of 65 to 75 years with exacerbated chronic bronchitis were treated with antibiotics: amoxycillin/clavulanic acid (20 patients), cefaclor (20 patients) and ciprofloxacin (20 patients). the treatment course in all the cases was 5 days. bacteriological tests of the sputum specimens and estimation of the isolate antibiotic susceptibility by the disk diffusion method were applied to all the patients before and after the treatment. 73 per cent of the patients had mixed inf ...	1997	9480649
structural analysis of compound i in hemoproteins: study on proteus mirabilis catalase.	ferryl catalysis has attracted considerable interest, because a diverse variety of enzymes use ferryl intermediates to perform difficult chemistry. the structure of the reactional intermediate compound i of proteus mirabilis catalase (pmc) has been solved using time-resolved x-ray diffraction techniques and single crystal microspectrophotometry. formation of compound i is characterized by significant changes in the absorbance spectrum, and the creation of an oxoferryl group on the distal side of ...	1997	9479449
sisyphus, proteus, and other characters in the odyssey of nursing science development.		1998	9479098
proteus meningitis in a child with occult spinal dysraphism.		1998	9477249
penetration of ciprofloxacin, norfloxacin and ofloxacin into the aqueous humours of patients by different topical application modes.	a prospective study was undertaken to determine the transcorneal penetration of three topically applied fluoroquinolones into aqueous humour.	1997	9476040
proteus syndrome. a case report of a hamartomatous syndrome with severe mandibular hemihypertrophy.	proteus syndrome is a rare congenital hamartomatous malformation that is characterized by a wide range of deformities, including craniofacial deformities. skin and skeletal developmental malformations are common and may assume tremendous proportions. the syndrome is often mistaken for other, more commonly recognized conditions, including neurofibromatosis. the soft-tissue masses in proteus syndrome are not nerve tumors but are usually hamartomatous proliferations. the case report describes its v ...	1998	9474620
studies on antimicrobial effect of the antihistaminic phenothiazine trimeprazine tartrate.	the antibacterial and bactericidal activities of the antihistamine trimeprazine were studied against 243 strains of bacteria which included both gram positive and gram negative types. the susceptibility of these bacterial strains to trimeprazine was assessed by determining their minimum inhibitory concentration (mic) which was found to be between 10 and 100 micrograms/ml. nineteen strains of staphylococcus spp. and salmonella spp. were trimeprazine. most of the strains belonging to bacillus spp. ...	1997	9468728
[neurophysiological aspects of proteus syndrome].	the proteus syndrome was defined in 1983 by wiedeman. however, the first case mentioned in the literature was that of joseph merrick, the elephant man, presented by sir frederick treves in 1884. it is a rare pathological condition. its multiple clinical features include; partial gigantism of hands and/or feet, pigmented nevi, hemihypertrophy of the body, tumors, skeletal anomalies, growth disorders and visceral anomalies. hereditary transmission has not been clearly defined. diagnosis and treatm ...	1997	9462983
[the results of experimental and clinical studies of a multicomponent vaccine made of the antigens from opportunistic microorganisms].		1997	9460881
proteus syndrome and distal arthrogryposis.		1997	9457508
proteus pyomyositis of the piriformis muscle in a swimmer.		1998	9455539
effect of anaerobic environment on the glutathione transferase isoenzymatic pattern in proteus mirabilis.	when proteus mirabilis was cultured anaerobically in the presence of nitrate as terminal electron acceptor, a dramatic reduction of glutathione transferase production occurred. the analysis of the glutathione affinity purified materials in terms of substrate specificity, sds-page pattern, ief pattern and immunoblotting revealed that a significantly different glutathione transferase pattern also occurred: two new glutathione transferase forms with an isoelectric point at ph 4.8 and 5.0 appeared. ...	1997	9453936
the first isolation of eubacterium suis in hungary.	eubacterium suis was isolated from the preputium of seven out of 16 mature boars on two farms and from the urinary bladder of one out of five sows originating from a third herd. the morphological and biochemical characteristics of the isolated strains were identical to that of the reference strain of e. suis attc 33144. three out of four strains isolated from farm a were successfully subcultured aerobically, and then anaerobically again. e. suis together with proteus mirabilis was isolated from ...	1997	9451944
degradation of commercial detergent products by microbial populations of the lagos lagoon.	the biodegradability potentials of three detergent products with the trade names omo, teepol and sodium dodecyl sulfate (sds) by the native bacteria of the lagos lagoon was carried out using the lagoon die-away method. physicochemical parameters of the water samples showed that the lagoon in apapa was more polluted than at the university of lagos. in 12 days, approximately 30, 60 and 97% of omo, teepol and sds respectively were degraded. sds with an alkyl sulfate moiety as surfactant supported t ...	1997	9449783
changing trends in frequency and antimicrobial resistance of urinary pathogens in outpatient clinics and a hospital in southern israel, 1991-1995.	in order to monitor changes in the frequency and antimicrobial resistance of urinary pathogens over several years, urinary cultures received from outpatient clinics and from a hospital during a period of one month each in 1991 and 1995 were analyzed at a clinical microbiology laboratory. in 1991 and 1995, 1366 and 1534 significant monomicrobic cultures respectively were reviewed. the frequency of escherichia coli dropped significantly in the outpatient clinics from 70.5% to 61.2% (p < 0.0001). t ...	1997	9447906
nitrate-reducing bacteria in diversion colitis: a clue to inflammation?	a pathogenic role of nitric oxide has been suggested in acute and chronic intestinal inflammation. we took the opportunity offered by studies in patients with excluded colon, which represents a model of chronic intestinal inflammation with no exogenous nitrite or nitrate supply, to evaluate the quantity and the quality of nitrate reducers in diversion colitis. thirty patients (17 men, 13 women, mean age 45 years) having an excluded colon for various reasons were sampled by rectal swabs and compa ...	1997	9440640
detection and speciation of bacteria through pcr using universal major cold-shock protein primer oligomers.	the detection of bacteria using pcr is a well-established diagnostic technique. however, conventional pcr requires the use of dna primer oligomers that are specific to the target organism and, as a consequence, a sample can only be tested for the presence of that specific target. a significant advantage would be to probe a sample for the presence of any bacteria, followed by identification. to achieve this it is necessary to identify a dna sequence common to all bacteria. here we demonstrate tha ...	1997	9439003
isolation and characterization of heavy metals resistant bacteria from lagos lagoon.	a total of 228 bacteria with an ability to resist toxic heavy metals were isolated from 8 selected sites of the lagos lagoon. the bacteria isolated were staphylococcus sp., bacillus sp., pseudomonas sp., streptococcus sp., moraxella sp., escherichia coli, proteus sp., klebsiella sp. and salmonella sp. the heavy metals to which resistance was recorded were mercury, lead, zinc, cobalt, copper and chromium. the lagoon sites from which the highest number of resistant bacteria were isolated were mari ...	1997	9438345
structural and immunochemical studies on the o-specific polysaccharide of proteus penneri strain 15.	on the basis of sugar analysis and 1h- and 13c-nmr spectroscopy, it was shown that the o-specific polysaccharide of proteus penneri strain 15 has a trisaccharide repeating unit, including an acetal-linked pyruvic acid residue, and is structurally identical to the capsular polysaccharide of proteus vulgaris strain atcc 49990. serological studies supported this conclusion and demonstrated the presence in the homological antiserum of both anti-core and anti-o chain antibodies reacting with a lipopo ...	1997	9437499
isolation, purification, and characterization of the major autolysin from pseudomonas aeruginosa.	the major (26 kda) autolysin from pseudomonas aeruginosa was purified to apparent homogeneity by a combination of preparative electrophoresis, ion-exchange, and dye-ligand chromatographies. this purification was facilitated by the development of a spot-assay that involved the spotting and subsequent incubation of autolysin samples on polyacrylamide gels containing peptidoglycan. the pl of the 26-kda autolysin was determined to be between 3.5 and 4 and disulfide bonds within the enzyme were essen ...	1997	9436306
a symbiont-produced protein and bacterial symbiosis in amoeba proteus.	gram symbiotic x-bacteria present in the xd strain of amoeba proteus as required cell components, synthesize and export a large amount of a 29-kda protein (s29x) into the host's cytoplasm across bacterial and symbiosome membranes. the s29x protein produced by e. coli transformed with the s29x gene is also rapidly secreted into the culture medium. inside amoebae, s29x enters the host's nucleus as detected by confocal and immunoelectron microscopy, although it is not clear if s29x is selectively a ...	1997	9435132
isolation and characterization of a cole1-like plasmid from enterobacter agglomerans with a novel variant of rom gene.	complete nucleotide sequence of a plasmid isolated from enterobacter agglomerans has been determined. the plasmid, called ppigdm1, consists of 2495 base pairs. the analysis of its nucleotide sequence suggested that ppigdm1 may be a cole1-like replicon. we confirmed this hypothesis by constructing a ppigdm1-derived plasmid harboring the cat gene (pbw4), which could be introduced into escherichia coli cells, and demonstrating that pbw4 cannot replicate in the absence of the pola function and that ...	1997	9435023
antibacterial activity of extracts and constituents of pelargonium sidoides and pelargonium reniforme.	the antibacterial activity of extracts and isolated constituents (scopoletin, umckalin, 5,6,7-trimethoxycoumarin, 6,8-dihydroxy-5,7-dimethoxycoumarin, (+)-catechin, gallic acid and its methyl ester) of pelargonium sidoides and pelargonium reniforme (geraniaceae), plant species used in folk medicine by the southern african native population, was evaluated against 8 microorganisms, including 3 gram-positive (staphylococcus aureus, streptococcus pneumoniae, and beta-hemolytic streptococcus 1451) an ...	1997	9434601
identification of a common structural motif in the disordered n-terminal region of bacterial flagellins--evidence for a new class of fibril-forming peptides.	flagellin proteins lacking the n- or c-terminus form polymers of reduced filament stability and straight morphology, in contrast to the coiled native flagella. in the present study, the n-terminal amino acid sequence of flagellins of the anaerobic beer spoilage bacteria pectinatus cerevisiiphilus and pectinatus frisingiensis as well as enterobacter aerogenes and pseudomonas sp. were determined. sequence similarity was revealed between these and the n-termini of all known eubacterial flagellins. ...	1997	9431986
pharmacokinetics of fosfomycin in chickens after a single intravenous dose and tissue levels following chronic oral administration.	this paper describes the pharmacokinetics of fosfomycin following a single i.v. bolus dose (10 mg/kg of body weight) in broiler chickens. serial blood samples were collected up to 5 h post-administration. fosfomycin serum concentrations were determined by a microbiological method, using proteus mirabilis as the test microorganism. the serum concentration versus time curves after i.v. administration followed a biexponential decline. the main pharmacokinetic variables were t1/2 lambda l = 23 min, ...	1997	9428152
use of green fluorescent protein to assess urease gene expression by uropathogenic proteus mirabilis during experimental ascending urinary tract infection.	proteus mirabilis, a cause of complicated urinary tract infection, expresses urease when exposed to urea. while it is recognized that the positive transcriptional activator urer induces gene expression, the levels of expression of the enzyme during experimental infection are not known. to investigate in vivo expression of p. mirabilis urease, the gene encoding green fluorescent protein (gfp) was used to construct reporter fusions. translational fusions of urease accessory gene ured, which is pre ...	1998	9423875
antimicrobial activity of micromeria nervosa from the palestinian area.	organic and aqueous solvent extracts and fractions of micromeria nervosa (desf.) benth. (labiatae) were investigated for antimicrobial activities on several microorganisms including bacteria and yeast. the different extracts differed significantly in their antimicrobial activities with the ethanolic extract being most active, followed by ethyl acetate and petroleum ether extracts. the least active extract was the aqueous. no significant difference between the different test microorganisms in the ...	1997	9421249
in vitro and in vivo antibacterial activities of gv129606, a new broad-spectrum trinem.	gv129606 is a new parenteral trinem antibiotic belonging to the beta-lactam class. it combines broad-spectrum activity (against gram-negative and -positive bacteria, aerobes and anaerobes), with high potency and resistance to beta-lactamases. comparative in vitro and in vivo antibacterial activities were determined for gv129606 against more than 400 recent clinical isolates (aerobes, including beta-lactamase producers, and anaerobes), using representative antibacterial agents (meropenem, piperac ...	1997	9420050
in vitro and in vivo antibacterial activities of cs-834, a novel oral carbapenem.	cs-834 is a novel oral carbapenem antibiotic. this compound is an ester-type prodrug of the active metabolite r-95867. the antibacterial activity of r-95867 was tested against 1,323 clinical isolates of 35 species and was compared with those of oral cephems, i.e., cefteram, cefpodoxime, cefdinir, and cefditoren, and that of a parenteral carbapenem, imipenem. r-95867 exhibited a broad spectrum of activity covering both gram-positive and -negative aerobes and anaerobes. its activity was superior t ...	1997	9420035
[analysis of the etiologic structure of urinary tract infection and antibiotic-resistance of its pathogens].	the main pathogens of inflammatory diseases of the kidneys and upper urinary tracts in inpatients of an urological unit were gramnegative organisms of the family enterobacteriaceae while the pathogens of the infection of the lower urinary tracts (nonspecific urethritis) and male genitalia were grampositive cocci. pseudomonas aeruginosa, enterobacter agglomerans and proteus spp. (indole positive) were the chief causative agents of the hospital infections. the analysis of the materials revealed a ...	1997	9412416
[development of resistance to difuracil (pap-49) in bacteria and its activity in combination with other antibacterial drugs].	possible increasing of resistance to difuracil or n-(5-nitrofurfuryliden)-5-nitrofuran-2-(n'-acetyl) carboxamidohydrazon (pap-49) in staphylococcus sp., streptococcus sp. and escherichia coli was studied and the level of the resistance increasing was determined by the method of 30-fold passage of the cultures in subbacteriostatic concentrations of the drug. it was shown in vitro that the increase of the resistance to the new nitrofuran was insignificant. the most intensive adaptation was observe ...	1997	9412411
[proteus infections].		1997	9411178
[bacterial flora in chronic inner ear infections in adults].	the aim of the study was to analyse microbiologically middle ear exudate obtained from 56 patients, aged 17 to 83 years, treated for chronic otitis media. aerobic bacteria only were found 49 patients (87,5%). mixed aerobic and anaerobic isolates were recovered from 7 patients (12,5%). the most common bacteria isolated from the middle ear exudate, in descending order frequency, were staphylococcus aureus (45%), pseudomonas aeruginosa (34%), proteus mirabilis (16%) and prevotella melaninogenica (9 ...	1997	9411077
[monotherapy with meropenem in febrile granulocytopenic patients].	infection remains the major cause of morbidity and mortality for cancer patients who become granulocytopenic. combinations of beta-lactams plus aminoglycosides have been the standard empiric therapy for febrile granulocytopenic patients, especially those with profound long-lasting granulocytopenia. the advent of new broad-spectrum cephalosporins and carbapenems has favoured the possibility of empiric monotherapy. meropenem is a parenteral carbapenem antibiotic stable to renal dehydropeptidase-i ...	1997	9410073
[meropenem: microbiologic perspective].	meropenem is a beta-lactamic carbapenem derived from thienamycin and is structurally characterized by the presence of a beta-methyl group in position c1 which confers stability to the molecule versus renal dehydropeptidase 1 (dhp-1), thereby making the coadministration of an enzyme inhibitor unnecessary. its esterochemical configuration of the lateral chain in c2 (dimethyl carbomoilpyrrolidenethium) increases the activity versus gram negative bacteria (enterobacteria and pseudomonas) and moreove ...	1997	9410064
transferable antibiotic resistance in nosocomial stenotrophomonas maltophilia strain.	stenotrophomonas maltophilia (298/85) was isolated from the extensively inflamed conjunctiva of a neonate in a regional hospital in ostrava, czech republic. it was resistant to all available antibiotics except cefepime and trimethoprim. the donor s. maltophilia strain 298/85 transferred carbenicillin and cephaloridine resistance determinants to recipient strains of escherichia coli k-12 3110 rif+ and proteus mirabilis p-38 rif+. all transconjugant colonies were co-resistant also to kanamycin, ce ...	1997	9401804
inhibition of motility and adherence of proteus mirabilis to uroepithelial cells by subinhibitory concentrations of amikacin.	the effect of subinhibitory concentrations of amikacin on proteus mirabilis motility and adherence to human uroepithelial and to hela cells was compared with that of gentamicin. in addition, the effect of both antibiotics on cell surface hydrophobicity was also examined. exposure of bacterial cells in the logarithmic phase to one fourth of amikacin or gentamicin at one fourth of their respective minimal inhibitory concentrations causes the inhibition of swarming and motility of proteus strains. ...	1997	9395856
antibiotic susceptibility of blood culture isolates of enterobacteriaceae from six norwegian hospitals 1991-1992.	from august 1991 to february 1992, each of the six largest hospitals throughout norway collected 84 to 107 consecutive blood culture isolates of enterobacteriaceae, altogether 571 isolates. the distribution of various species and genera at the different hospitals was uniform; escherichia coli being most prevalent (57-67%), followed by klebsiella spp. (12-18%) and proteus mirabilis (7-11%). twenty-one and 4% of e. coli isolates were resistant to ampicillin and cefuroxime, respectively, and 11% of ...	1997	9393556
[etiological investigation on diarrhoeal diseases in the rural area of suixian county, yu dong plain in china].	to study the etiology of diarrhoeal diseases in the rural area, we carried out a study on diarrhoeal diseases in two natural villages. 15 kinds of diarrhoeal pathogens including 172 strains were isolated from 318 patients with diarrhoea making the isolation rate 49.37%; 12 kinds of diarrhoeal pathogens and 69 strains were isolated from 310 healthy controls and the isolation rate was 22.26%; 6 kinds of diarrhoeal pathogens and 44 strains were isolated in 405 samples obtained from the envronmenfal ...	1996	9387573
necrotizing fasciitis: improved survival with early recognition by tissue biopsy and aggressive surgical treatment.	necrotizing fasciitis is a soft tissue gangrenous infection that is optimally treated by early diagnosis, radical surgical debridement of all involved necrotic tissue, broad spectrum antibiotics, and aggressive nutritional support. the early clinical diagnosis of an area of necrotizing fasciitis is difficult and frequently unreliable. we are reporting a series of cases in which an early, accurate diagnosis of necrotizing fasciitis was established by a frozen section tissue biopsy obtained at the ...	1997	9386043
[simultaneous use of different nuclear medical examinations in klippel-trenaunay syndrome--vs. proteus syndrome].	a three-year-old male patient presented already at his birth a disproportion macrosomia of the left foot and a large, nodular nevus flammeus in the left hip region, which led to the tentative diagnosis of a klippel-trenaunay syndrome. in the following years, both changes showed a continuous progression, with distinct soft-tissue swelling as well as papillomatous and verruciform vegetations of the nevus. additionally, large, plain subcutaneous masses developed under the right shoulder, and a macr ...	1997	9380531
[characterization of plasmids which mediate resistance to multiple antibiotics in gram-negative bacteria of nosocomial origin].	the genetic and molecular mechanisms involved in antimicrobial resistance of 10 strains of gramnegative bacilli (1 serratia marcescens; 2 escherichia coli; 1 proteus mirabilis; 4 klebsiella pneumoniae; 1 enterobacter cloacae y 1 alcaligenes faecalis), isolated from adult patients with nosocomial pulmonary infection at the in-patient facilities of the university hospital of los andes, mérida, venezuela, have been studied.	1997	9376400
igm, igg and iga class enterobacterial antibodies in serum and synovial fluid in patients with ankylosing spondylitis and rheumatoid arthritis.	igm, igg and iga class antibodies against three klebsiella pneumoniae capsular types, escherichia coli and proteus mirabilis, as well as total immunoglobulin concentrations, were measured by enzyme immunoassay and radial immunodiffusion technique, respectively, in paired serum and synovial fluid samples from eight patients with ankylosing spondylitis and 10 with rheumatoid arthritis. no clear evidence for intra-articular antibody production against any of the studied microbes was found.	1997	9374920
the simultaneous production of both hly- and hpm-like hemolysins is characteristic of the proteus penneri species.	clinical isolates of proteus penneri were tested for the presence of genes encoding hemolytic activity. strains possessing dna sequences similar to the hlycabd genes in escherichia coli were found. each secreted a 110 kda protein which reacted with a specific anti-hlya antiserum. southern blotting analysis revealed that the hindiii restriction fragment pattern for the hlycabd genes of these strains was conserved. similarly, the chromosomal location of these genes is relatively conserved based on ...	1997	9373950
comparative in vitro evaluation of piperacillin/tazobactam in a tertiary care hospital.	bacterial resistance is usually a serious problem in tertiary care hospitals. the aim of this in vitro study was to evaluate the beta-lactamase inhibitor combination piperacillin/tazobactam in a hospital environment with high bacterial resistance rates and compare it with other beta-lactam agents. three hundred and sixty-two isolates from various clinical materials were studied during the period march-august 1996. material for culture was collected from patients of all the wards of our hospital, ...	1997	9373788
characterization of the pena and penr genes of burkholderia cepacia 249 which encode the chromosomal class a penicillinase and its lysr-type transcriptional regulator.	burkholderia cepacia is recognized as an important pathogen in the lung infections of patients with cystic fibrosis. an inducible beta-lactamase activity has been associated with increased resistance to beta-lactam antibiotics in clinical isolates of b. cepacia. in this study, we report the revised sequence of the pena gene, which encodes the inducible penicillinase of b. cepacia, and show that it belongs to the molecular class a beta-lactamases and exhibits a high degree of similarity to the ch ...	1997	9371340
ofloxacin otic solution for treatment of otitis externa in children and adults.	to compare the safety and efficacy of ofloxacin otic solution with those of cortisporin otic solutions (neomycin sulfate, polymyxin b sulfate, and hydrocortisone) in otitis externa in adults and children.	1997	9366699
production and characterisation of monoclonal antibodies to heat-shock protein 60 of helicobacter pylori.	two monoclonal antibodies (mabs), designated as h9 (igg2a) and h20 (igm), directed against heat-shock protein 60 (hsp60) of helicobacter pylori strain tk1029 were established. affinity-purified antigens cross-reacted in immunoblots with mab h9 and mab h20 respectively. these antigens also reacted with the 3c8 mab previously established in this laboratory, which recognised yersinia enterocolitica hsp60. by amino-acid sequence analysis, the n-terminal amino-acid sequence of the protein recognised ...	1997	9364137
unilateral hydronephrosis, pyelonephritis, and bacteremia caused by a neglected vaginal ring pessary.		1997	9361677
an infection inhibiting urinary catheter material.	catheter associated bacteriuria is a common infection in hospitals and nursing homes. an infection inhibiting catheter material for fabricating urinary catheters is being developed. the material consists of silicone rubber elastomer compounded with chlorhexidene gluconate (chg) matrix. the antibiotic is released in sustained fashion over at least 4 weeks. a method was established for adding chg to silicone rubber. to protect the chg, it is suspended in a water soluble wax that also modulates chg ...	1997	9360165
focal xanthogranulomatous pyelonephritis: partial nephrectomy as definitive treatment.	we report 11 cases of focal xanthogranulomatous pyelonephritis (fxgp), a disease that is very uncommon. the aim of the present work is to assess the effectivity of conservative treatment.	1997	9358230
sensitivity to the bactericidal effect of human serum of proteus strains from clinical specimens.	a total of 257 proteus strains isolated from urinary tract infection, blood, wound and faeces were studied. of the strains tested 31 (12 percent) were serum sensitive, 182 (71 percent) were serum resistant and the remaining 44 (17 percent) showed intermediate sensitivity to the pooled normal human serum (pnhs). strains isolated from adult urines and blood cultures were significantly more sensitive than strains of faecal origin (p < 0.01). no significant difference was seen between strains from f ...	1997	9354003
novel selective and non-selective optical detection of microorganisms.	a new instrument, capable of detecting metabolic changes due to microbiological activity, is described. optical changes in growth media are monitored in a semi-fluid zone that separates the liquid medium containing the sample. data demonstrate that common media can be utilized in conjunction with this rapid automated technology. nutrient broth with the ph dye indicator. bromocresol purple was suitable for total counts. selective media containing dyes were utilized to assess the presence or absen ...	1997	9351264
evaluation of bbl chromagar orientation medium for detection and presumptive identification of urinary tract pathogens.	the microbiological performance of bbl chromagar orientation medium and cps id2 agar was compared to that of columbia agar with 5% sheep blood and macconkey agar without crystal violet for the enumeration and presumptive identification of bacteria responsible for urinary tract infections. of a total of 658 clinical urine specimens, 118 specimens yielded no growth, 402 specimens yielded growth with cell counts of > or = 10(5) cfu/ml, and 138 specimens yielded growth with cell counts of < 10(5) cf ...	1997	9350731
[the photometric determination of the antilysozyme activity of microorganisms].	the method for the determination of the antilysozyme activity (ala) of microorganisms, based on the photometric determination of the residual activity of enzyme with the use of micrococcus lysodeikticus test culture after the incubation of the strain under test and lysozyme, is proposed. the new method enhances the reliability of the determination of the ala of microorganisms due to an increase in the accuracy of the quantitative determination of ala. the elimination of the stage of interaction ...	1997	9340990
concentrations of prophylactic ceftriaxone in abdominal tissues during pancreatic surgery.	ceftriaxone concentrations in abdominal tissues were evaluated after administration as antibiotic prophylaxis for pancreatic surgery. ten patients were given ceftriaxone (1 g i.v.) 30 min before surgery. ceftriaxone concentrations in fatty tissues ranged from 2.5 to 6.2 microg/g. ceftriaxone concentrations were 6.0 +/- 8.6 microg/g in pancreatic tissues, 2.1 +/- 2.5 mg/l in pancreatic fluid, 1179 +/- 1271 mg/l in pancreatic bile, and 18 +/- 16 microg/g in the liver. in fatty tissues, 8-10 patien ...	1997	9338502
permeability to carbapenems of proteus mirabilis mutants selected for resistance to imipenem or other beta-lactams.	an imipenem-resistant mutant of proteus mirabilis lacked a 26 kda outer membrane protein (omp). it has previously been postulated that this protein is a porin, but the present mutant, which was cross-resistant to mecillinam but not to other beta-lactams, proved as permeable to carbapenems as its parent. a mecillinam-selected mutant had similar cross-resistance yet retained the 26 kda omp, confirming that this protein was not important to resistance. in contrast, cefoxitin-selected mutants retain ...	1997	9338488
[evaluation of in vitro activity of new fluoroquinolones, cephalosporins and carbapenems against 569 gram-negative bacteria].	evaluation of the in vitro activity of new fluoroquinolones, cephalosporins and carbapenems against gram-negative bacteria.	1997	9336049
[torus mandibularis].	solitary or bilateral, symptomless exostoses on the lingual surface of the mandibule are called mandibular torus. it is mainly seen in young males and has a benign clinical course. the etiopathology is not known. both genetic and environmental factors such as the anatomy of the lower jaw are considered. syndromes associated with facial exostoses such as proteus syndrome or gardner's syndrome should be clinically excluded. a 40-year-old man with exostoses of the jaw is reported. with this case re ...	1997	9333619
in vitro evaluation of bo-3482, a novel dithiocarbamate carbapenem with activity against methicillin-resistant staphylococci.	bo-3482, a dithiocarbamate carbapenem, inhibited clinical isolates of methicillin-resistant staphylococci (mrs) at 6.25 microg/ml (mic at which 90% of isolates tested are inhibited [mic90]), while the mic90 of imipenem was > 100 microg/ml. bo-3482 was generally less active than imipenem against methicillin-susceptible staphylococcus aureus, streptococci, enterococci, and gram-negative bacteria, although bo-3482 showed better activity (mic90) than imipenem against enterococcus faecium, haemophilu ...	1997	9333063
antimicrobial potentiality of a new beta-lactum antibiotic fosfomycin.	antimicrobial action of penicillin and some of its derivatives including fosfomycin was studied with respect to 225 strains of gram-positive and gram negative bacteria. fosfomycin was found to possess somewhat less activity against staphylococcus aureus compared with other penicillins; however, it showed powerful activity towards escherichia coli, klebsiella spp. and proteus mirabilis.	1997	9332178
population-specific behavioral electrosensitivity of the european blind cave salamander, proteus anguinus.	in nine salamanders from different slovenian populations of the urodele proteus anguinus, including three specimens of its 'black' variety, p anguinus parkelj, thresholds of an overt avoidance response to electrical field stimuli were estimated as a function of frequency (continuous sine-waves in water). thresholds down to 0.3v/cm (ca 100 na/cm2) and up to 2 mv/cm (670 na/cm2), at 'best frequencies' of around 30 hz were found. sensitivity covered a total frequency range of below 1 hz, excluding ...	1997	9326735
five-year prospective study of bacteraemic urinary tract infection in a single institution.	in order to determine the epidemiology, microbiology, and outcome of bacteraemia originating in the urinary tract in hospitalised patients, a prospective study was conducted in a large general hospital in israel. data from all patients with bacteraemia were collected prospectively, and a subgroup of patients with bacteraemia secondary to urinary tract infection was analysed. there were 702 episodes of bacteraemia secondary to urinary tract infection during a five-year period (33.9% of all episod ...	1997	9323466
splenic abscess: a rare sequel to renal abscess caused by staghorn calculi.		1997	9313683
evidence for symbiont-induced alteration of a host's gene expression: irreversible loss of sam synthetase from amoeba proteus.	symbiont-bearing xd amoebae no longer produce a 45-kda cytoplasmic protein that functions as s-adenosylmethionine synthetase in symbiont-free d amoebae. the absence of the protein in xd amoebae is attributable to xd amoeba's failure to transcribe the corresponding gene as a result of harboring bacterial symbionts. however, xd amoebae have about half the level of enzyme activity found in d amoebae, indicating that they use an alternative source for the enzyme. xd amoebae originated from d amoebae ...	1997	9304810
the expression of nonagglutinating fimbriae and its role in proteus mirabilis adherence to epithelial cells.	proteus mirabilis is a common causative agent of human urinary tract infections, especially in catheterized patients and in those patients with structural abnormalities of the urinary tract. in addition to the production of hemolysin and urease, fimbriae-mediated adherence to uroepithelial cells and kidney epithelium may be essential for virulence of p. mirabilis. a single p. mirabilis strain is capable of expressing several morphologically distinct fimbrial species, which can each be favoured b ...	1997	9304781
a motile but non-swarming mutant of proteus mirabilis lacks flgn, a facilitator of flagella filament assembly.	a tnphoa mutant of proteus mirabilis was isolated, which had lost the ability to swarm, yet was still motile. the transposon had inserted into flgn, a flagella gene encoding a 147-amino-acid protein of undefined function. proteus flgn is arranged in an operon with the class iii anti-sigma28 gene, flgm, flanked by the class ii genes, flga, flgbcd and flhba, and a novel putative virulence-related gene. the flgn mutation caused a substantial reduction in cell surface-associated flagellin, particula ...	1997	9302021
characterization and amino acid sequence analysis of a new oxyimino cephalosporin-hydrolyzing class a beta-lactamase from serratia fonticola cuv.	serratia fonticola cuv produces two isoenzymes (forms i and ii) with beta-lactamase activity which were purified by a five-step procedure. the isoenzymes had identical kinetic parameters and isoelectric point (pi = 8.12). they were characterized by a specific activity towards benzylpenicillin of 1650 u/mg. the beta-lactamase hydrolyzed benzylpenicillin, amoxycillin, ureidopenicillins, first- and second-generation cephalosporins. carboxypenicillins and isoxazolylpenicillins were hydrolyzed to a l ...	1997	9300809
[study of in vitro activity of 3 cephalosporins and their combination with 2 beta-lactamase inhibitors against 4 enterobacteria strains producing extended spectrum beta-lactamase].	the in vitro antibacterial activity of cefotaxime, cefepime and cefpirome was evaluated alone and in association with two beta-lactamase inhibitors: clavulanic acid and sulbactam against 65 extended broad spectrum beta-lactamase producing enterobacteriaceae strains [klebsiella pneumoniae (35), proteus mirabilis (9), escherichia coli (11), enterobacter aerogenes (10)]. the lowest mics were observed for cefepime and cefpirome used alone. in association with inhibitors cefotaxime was less effective ...	1997	9296097
[bactericidal activity determination of biseptine, combination of chlorhexidine, benzalkonium chloride and benzylic alcohol, on 124 hospital bacterial strains].	biseptine is an antiseptic composed of chlorhexidine digluconate (chx), benzalkonium chloride (bc) and benzylic alcohol. minimal bactericidal concentrations (mbcs) of biseptine were determined on 124 clinical strains: 76 enterobacteriaceae, 16 other gram negative bacilli, (pseudomonas spp, aeromonas spp, haemophilus spp) and 32 gram positive bacteria (staphylococcus spp, streptococcus spp, listeria spp, bacillus cereus), using microdilution method, in comparison with hibitane champ. modal mbc of ...	1997	9296082
chemotaxis of amoeba proteus in the developing ph gradient within a pocket-like chamber studied with the computer assisted method.	a new "u" shaped, pocket-like chamber was used to observe the chemotactic responses of individual cells. this method permits monitoring of both the development of the concentration gradient of a tested substance and cell locomotion. we investigated the chemotactic responses of amoeba proteus and observed that the amoebae moved in positively and negatively developing [h+] gradients towards the solution of lower ph in a ph range 5.75-7.75. the chemotactic response of amoebae to [h+] gradients requ ...	1997	9295140
infectious temporomandibular joint disease in the horse: computed tomographic diagnosis and treatment of two cases.	diagnostic imaging and treatment of unilateral destructive temporomandibular joint disease in two horses is described and discussed. computed tomography appeared to be the best imaging technique for these lesions. the disease can be followed by functional recovery after the infection has resolved.	1997	9290196