Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| dynamics of the interaction between entamoeba histolytica and components of the immune response. | trophozoites of entamoeba histolytica redistributed specific antibodies reacting to antigens on their surface. the presence of a second layer of anti-antibodies enhanced the redistribution of anti-amoeba antibodies but was not a prerequisite for this process. microfluorometric measurements of the fluorescence intensity in different areas of the cell surface provided objective evidence for the antibody-induced redistribution. in order to distinguish surface-bound and internalized anti-amoeba anti ... | 1980 | 6258222 |
| purification and properties of nadph:flavin oxidoreductase from entamoeba histolytica. | amebal nadph:flavin oxidoreductase was purified to apparent homogeneity. molecular weights of 40 000 and 38 000 were estimated by gel filtration and by sodium dodecyl sulfate polyacrylamide gel electrophoresis, respectively, indicating that the enzyme is composed of a single polypeptide chain. the enzyme does not contain firmly bound flavin. it exhibited 20-fold selectivity for nadph over nadh. with the former donor it reduced riboflavin, galactoflavin, fmn, or fad. aerobically the reducing equi ... | 1980 | 6258069 |
| incorporation of glucose carbon into ribonucleotides of axenic entamoeba histolytica. | axenic entamoeba histolytica grown with (ul-14c)glucose in tp-s-1 medium were capable of biosynthesizing ribose from labeled glucose. rna isolated by phenol extraction was hydrolyzed to the ribonucleotide level by alkaline hydrolysis. the hydrolysate, chromatographed on ion exchange resins, yielded amp, gmp, and ump, but not cmp containing labeled glucose carbon. the present nucleotide composition of the isolated amebal rna was, respectively, as follows, cmp, 0.20; gmp, 0.22; amp, 0.30; ump, 0.2 ... | 1980 | 6257878 |
| studies on the examination of imported laboratory monkey, macaca fascicularis for e. histolytica and other intestinal parasites. | infection rate of imported monkeys, under this study, with intestinal protozoa is higher than the infection rate with intestinal nematodes. mostly encountered protozoa were e. coli, e. nana, e. histolytica and i. butschlii. formalin-ether concentration method yielded higher % positive for e. histolytica than the culture method using tanabe-chiba medium, but the results obtained from these 2 methods correlate perfectly well with each other. efficacy of thiabendazole against intestinal nematodes s ... | 1980 | 6257621 |
| [do hepatotropic strains of entamoeba histolytica exist?]. | liver amoebic abscess often occur without any colitic symptoms nor any parasite detection in the colon. the localisation heterogeneity and the experimental data, associated with clinical expression suggest the existence, inside the species e. histolytica, of various strains: non pathogenic one, invasive for the intestine mucous membrane ones and invasive for liver tissue ones. | 1980 | 6257412 |
| amebiasis. a persistent problem. | 1981 | 6255883 | |
| an immunofluorescence test to detect serum antibodies to giardia lamblia. | we used an indirect immunofluorescence test with giardia lamblia trophozoites as antigen to detect anti-g. lamblia antibodies in serum. seventy-one patients and control subjects were studied in a blinded protocol. titers in 29 of 30 patients with symptomatic giardiasis (1:16 to 1:1024) did not overlap titers in 19 healthy control subjects (1:2 to 1:4); titers in 15 patients with hookworm, entamoeba histolytica, or intestinal bacterial overgrowth were 1:16 or less absorption of giardiasis patient ... | 1980 | 6255845 |
| niacin requirement for growth of axenic entamoeba histolytica. | niacin (nicotinic acid) was found to be essential for the cultivation of axenic entamoeba histolytica. this vitamin requirement was also satisfied by nicotinamide. panmede liver digest, a source of vitamins in the axenic medium was replaced with a dialyzed hot water extract of homogenized whole liver, supplemented with various growth factors. cultured medium made with the liver extract and not supplemented with niacin failed to support continued multiplication of e. histolytica, but did support ... | 1980 | 6255809 |
| protective effects of crude and chromatographic fractions of axenic entamoeba histolytica in guinea-pigs. | axenic amoebic antigen was chromatographed on sephadex g-200. two peaks divided into three fractions were obtained which were concentrated by lyophilization. guinea-pigs were given a series of subcutaneous injections of crude entamoeba histolytica antigen and its chromatographic fractions mixed with freund's complete adjuvant. after three immunizing doses, the animals were challenged with intracaecal injection of a virulent isolate of amoebae (strain no. h-25) and were killed seven days later. t ... | 1980 | 6255636 |
| a comparative study of entamoeba histolytica (nih :200, hk9, etc.), "e. histolytica-like" and other morphologically identical amoebae using isoenzyme electrophoresis. | cultures of old, well documented strains of entamoeba histolytica (nih:200, hk9 etc.) have been compared with "e. histolytica-like" strains and, in turn, with e. moshkovskii, e. invadens and e. chattoni. they have been grouped according to their isoenzyme patterns. the "e. histolytica-like" organisms give patterns similar to e. moshkovskii and both of these are different from e. histolytica groups i to iv. e. invadens and e. chattoni have easily distinguished characteristic isoenzyme bands. "e. ... | 1980 | 6255635 |
| mitogenicity of entamoeba histolytica extracts for murine lymphocytes. | aqueous extracts or aqueous extracts of delipidated entamoeba histolytica (e.h.e.) contain a mitogenic principle for murine lymphocytes. as detected by [3h]-thymidine incorporation and blast transformation, e.h.e. acted predominantly on t cells of splenic origin, but not on thymocytes or bone marrow cells. furthermore, e.h.e. induced proliferation of a subset of non-t-cells which is present in the spleen of athymic nude mice, adhered to nylon wool, but could not be activated to produce antibody. ... | 1980 | 6254869 |
| surface coagglutination with formalinized, stained protein a staphylococci in the immunologic study of three pathogenic amebae. | the indirect immunofluorescence and, to a lesser extent, the immunoperoxidase methods have become the principal means of conducting immunological studies upon amebae. these procedures require the conjugation of fluorescein or enzyme with antiglobulin reagents and fixation of the amebae. the present report describes a somewhat similar methodology wherein formalinized, stained protein a staphylococci combined with specific antibody have been found to coagglutinate upon a portion of the amebic surf ... | 1980 | 6254383 |
| protection against amebic liver abscess in hamsters by means of immunization with amebic antigen and some of its fractions. | immunization of hamsters by intramuscular injections of an amebic extract and of two of its fractions, emulsified with complete freund's adjuvant, resulted in protection against the development of amebic liver abscess, following intrahepatic inoculation of axenic trophozoites of entamoeba histolytica such that fraction i, fraction ii, and the whole antigen conferred protection to 18 out of 18, 4 out of 18, and 11 out of 18 hamsters, respectively. splenomegaly was found to accompany the developme ... | 1980 | 6254382 |
| comparison of iha test for amoebiasis on serum and filter paper specimens. | 1980 | 6254220 | |
| experimental amoebic infection in guinea-pigs immunized with low grade amoebic infection. | guinea-pigs were immunzied with a low grade amoebic infection, either intracaecally or intramesenterically. the immunized animals were challenged with a virulent strain of entamoeba histolytica. some of the animals, immunized via the intracaecal route were challenged repeatedly. 10 to 30% of immunized animals developed caecal lesions compared with 92.8% of non-immunized animals. the percentage of protection in animals challenged repeatedly was greater than in those challenged once. neither immun ... | 1980 | 6254217 |
| amoebic antigen in immunodiagnosis and prognosis of amoebic liver abscess. | the detection of amoebic antigen by counterimmunoelectrophoresis is very useful and important in the immunodiagnosis of invasive hepatic amoebiasis. the antigen was demonstrated in 115 (92%) of 125 patients with amoebic liver abscess. all the 19 cases which showed entamoeba histolytica in the 'pus' were positive for the antigen and 96 of 106 samples negative for the parasite by smear and culture were also positive for the antigen. in none of the controls was a falsepositive reaction obtained. 12 ... | 1980 | 6254216 |
| immunogenicity of axenic entamoeba histolytica antigen and its fractions. | amoebic antigen prepared from axenic entamoeba histolytica (nih:200 strain) grown at 37 degrees c in tps i medium was chromatographed on a sephadex g-200 column. three fractions (f-i, f-ii, f-ii) were obtained, the molecular weights of which ranged from 650,000 to 1,450. fractions were concentrated by lyophilization and antisera against each fraction and whole antigen were raised in rabbits. partially purified fractions were used in various serological tests and their reactivities were compared ... | 1980 | 6254215 |
| occurrence of hepatic amoebiasis in rats inoculated intracaecally with a virulent strain of entamoeba histolytica. | 1980 | 6254213 | |
| entamoeba histolytica: cytopathogenicity, including serum effects on contact-dependent and toxin-induced lysis of hamster kidney cell monolayers. | 1980 | 6253314 | |
| sequential histopathology of cavitary liver abscess. formation induced by axenically grown entamoeba histolytica. | multiple hamster liver passage of entamoeba histolytica trophozoites with intervening recovery into axenic culture caused increased virulence as measured by increase in the size of the lesion produced. lesions produced by amebae that had not been liver-passaged did not persist; however, multiply liver-passaged substrains produced large, fluid-filled abscesses one month to six weeks after inoculation. six days after inoculation, lesions consisted of multiple granulomas, lymphocytes, and e histoly ... | 1980 | 6252861 |
| cell mediated immune responses in experimental amebiasis. | cell migration was inhibited when entamoeba histolytica antigen-sensitized peritoneal exudate cells were cultured in the presence of specific antigen. the inhibition of cell migration was caused due to interaction of antigen with sensitized lymphocytes. this migration inhibition was well correlated with cell mediated immune responses, but not with circulating antibody levels. peritoneal cells obtained from animals immunized with 70.5 microgram of antigen gave a better response in capillary tube ... | 1980 | 6252860 |
| activity of quinfamide against natural infections of entamoeba criceti in hamsters: a new potent agent for intestinal amoebiasis. | a novel tetrahydroquinolinyl ester, quinfamide, administered orally in multiple doses for 3 days had an ed50 of 0.25 mg/kg/day (total dose 0.75 mg/kg) for eradicating entamoeba criceti in hamsters in several tests. it was significantly more active by direct comparison than 3 commercially available amoebicides and at least as active as 2 other esters of the parent compound, 1-(dichloroacety)-1,2,3,4-tetrahydro-6-quinolinol. after administration of a single dose, ed50 calculations for quinfamide a ... | 1980 | 6252530 |
| application of thin layer immunoassay (tia) for demonstration of antibodies against entamoeba histolytica. | thin layer immunoassay (tia) was used to demonstrate antibodies against entamoeba histolytica in sera from patients and blood donors. the tia results agreed well with those obtained by the indirect hemagglutination (iha) and immunodiffusion (id) techniques. it is suggested that because of its technical simplicity and low cost tia may be used alternatively or in addition to the iha and id techniques for screening patient sera for antibodies to e. histolytica. however, the new technique must first ... | 1980 | 6250415 |
| hepatic and thoracic amaebiasis. | sonographic and radiographic fidings were reviewed in 27 patients with hepatic amebiasis. sonography usually demonstrated nonspecific, peripheral, hypoechoic lesions. the only diagnostic sonographic appearance was a combination of a hypoechoic lesion and diaphragmatic disruption, which was found in four patients. about 50% of the patients had accompanying radiographic abnormalities that were nondiagnostic. these included elvation of the right hemidiaphragm, basilar pulmonary infiltrates, and ple ... | 1980 | 6250386 |
| cell-mediated immune response in amebiasis. | studies on cell-mediated immune response (cmir) to amebia antigen in amebic liver abscess showed conflicting results. the leucocyte migration test was employed in nine cases of amebic liver abscess and cmir to amebic antigen was demonstrated in four cases. the role of cmir in the pathogenesis of amebic liver abscess remains unknown but its presence is confirmed. | 1980 | 6250227 |
| attachment and short-term maintenance of motility and viability of entamoeba histolytica in a defined medium. | organic requirements for attachment to glass, elongation, and motility of entamoeba histolytica, have been determined. the trophozoite, which has been grown axenically only in highly complex media with reduced oxygen tensions, remains rounded and detached when placed in a tris-hcl buffered solution containing nacl, kcl, mgcli, and cacl2. a maintenance medium in which the amebae could attach to glass, elongate, and remain motile and viable for 12 to 24 h was devised with the addition of cysteine, ... | 1980 | 6249921 |
| plasma membrane of entamoeba histolytica. | axenically propagated entamoeba histolytica (hk9:nih strain) were employed as starting material for the isoation of plasma membrane by a novel procedure. in the absence of known enzymatic markers, the externally disposed polypeptides of intact amoebae were iodinated and the incorporated label used to monitor membrane separation and recovery. 12 major plasma membrane polypeptides (12 x 10(3)-200 x 10(3) mol wt) were labeled and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresi ... | 1980 | 6249883 |
| cytopathogenic mechanisms of entamoeba histolytica. | cinemicrography of entamoeba histolytica destruction of chinese hamster ovary (cho) cells shows that ameba cytopathogenicity consists of separate components: a contact-dependent cytolethal effect, and phagocytosis. cells not in contact with amebae remain intact. quantitation of ameba destruction of cho cells by applying the one-hit hypothesis confirms that the cytoethal effect of amebae is contact dependent. studies with 111indium oxine-labeled cells provide further evidence of extracellular kil ... | 1980 | 6249882 |
| lectin activity in entamoeba histolytica trophozoites. | a lectin (carbohydrate-binding protein) has been found in extracts of a number of axenically grown trophozoites of entamoeba histolytica strains. the strains grown in tyi-s-33 medium (diamond et al., trans. r. soc. trop. med. hyg. 72: 431-432, 1978) were hk-9, 200:nih, and hm-1:imss. strain hu-1:musc (hsc) was grown monoxenically in the same medium. the amoebic lectin agglutinated glutaraldehyde-fixed erythrocytes. this activity was ph dependent and heat and oxidation sensitive, and was destroye ... | 1980 | 6249757 |
| respiratory metabolism of giardia lamblia. | 1980 | 6248623 | |
| hepatic amoebiasis in immunodepressed mice. | the effects of immunodepression on intestinal amoebic lesions produced by entamoeba histolytica were studied in inbred mice of the c3h/mg strain. immunodepression was induced in two ways by giving (i) cyclophosphamide and (ii) anti-mouse lymphocyte serum. on immunodepression with the latter, although there was no difference in the degree of macroscopic intestinal damage, all five surviving immunodepressed mice showed hepatic lesions. it is noted that these had a striking histological resemblance ... | 1980 | 6247788 |
| [amebic liver abscess : value of extemporaneous search for ameba]. | 1980 | 6246317 | |
| entamoeba histolytica and entamoeba invadens: effects of temperature and oxygen tension on growth and survival. | 1980 | 6245905 | |
| [use of an enzymatic immune method (elisa) for the serodiagnosis of amebiasis]. | the enzyme linked immunosorbent assay (elisa) was compared with the indirect immunofluorescence antibody test (ifat) and with the counter-immunoelectrophoresis (cie) in the serology of amebiasis. in cases of liver abscesses, the elisa was as sensitive as cie, but slightly more sensitive than ifat. in cases where the patient asymptomatically passed cysts of e. histolytica, none of the three tests was positive. as a routine laboratory screening test, the elisa proved to be the most convenient of t ... | 1980 | 6245444 |
| [survival of entamoeba histolytica in pus from a liver abscess]. | 1980 | 6245405 | |
| amebic abscess of the brain. | brain abscesses caused by entamoeba histolytica were treated in two residents of new jersey, neither of whom had traveled beyond the eastern united states. the diagnosis in case 1 was confirmed by the presence of ameba in both brain and pituitary abscesses. an elevated indirect hemagglutination (iha) titer of 1:4096 and response to specific treatment with metronidazole and chloroquin were the criteria for diagnosis in case 2. the computerized tomographic appearance of an amebic abscess is that o ... | 1980 | 6245387 |
| immunoglobulin e and a levels in atopic and anaphylactic children. | seventy-six children between 18 months and 12 years of age were included in this study. sixty-one were atopic and 15 had anaphylaxis due to bee stings or drugs. immunoglobulins: a, g. m and e were determined and evaluated with respect to age and diagnosis. iga levels were lower in atopic children between six and ten years than in normals. ige levels were high in both atopic and anaphylactic patients but higher in the atopic than in the anaphylactic group. the results are discussed, focusing on t ... | 1980 | 6243451 |
| surface redistribution and release of antibody-induced caps in entamoebae. | polyspecific antibodies bound to entamoeba induced surface redistribution of membrane components toward the uroid region. capping of surface antigens was obtained with a single layer of antibodies in e. histolytica and e. invadens. this surface segregation progressed to a large accumulation of folded plasma membrane that extruded as a defined vesicular cap. a spontaneous release of the cap at the end of the capping process took place. these released caps contained most of the antibodies that ori ... | 1980 | 6243156 |
| entamoeba histolytica: isolation of ferredoxin. | 1980 | 6243106 | |
| entamoeba histolytica: nucleic acid precursors affecting axenic growth. | 1980 | 6243105 | |
| schistosoma mansoni, s. haematobium, and s. japonicum: identification of genus- and species-specific antigenic egg glycoproteins. | immunoreactive egg glycoproteins of schistosoma mansoni, s. haematobium, and s. japonicum which are genus- and species-specific, or react with sera of patients infected with other parasites, have been identified. egg proteins were labeled with iodine-125, and the concanavalin a-binding glycoproteins were immunoprecipitated with sera of patients infected with one of four species of schistosoma or trichinella spiralis, taenia solium, echinococcus granulosus, entamoeba histolytica, or wuchereria ba ... | 1984 | 6209162 |
| endocytosis in entamoeba histolytica. evidence for a unique non-acidified compartment. | our studies on endocytosis in entamoeba histolytica trophozoites suggest that there are two vacuolar compartments in this organism. the first compartment consists of large vacuoles (greater than 2 microns diameter). as measured by the fluid phase markers, fluorescein isothiocyanate (fitc)-dextran and horseradish peroxidase (hrp), this compartment is a rapid equilibrium with the external milieu and is constantly exchanging (1-2 h) its contents with the external medium. the contents of these vacuo ... | 1984 | 6206186 |
| immunogenicity of rna-protein fraction isolated from axenic entamoeba histolytica (nih:200). | the immunogenicity of entamoeba histolytica rna (ehrna) was studied in guinea-pigs. animals immunized with a single dose of ehrna and freund's complete adjuvant (fca) showed a high level of cell-mediated immune response (cmir) as assessed by percent leucocyte migration inhibition (74.8 +/- 2.9) after 1 week of immunization. this response faded away gradually and by week 5 after immunization the percent leucocyte migration inhibition was 27.1 +/- 8.27, which is quite close to control values. the ... | 1984 | 6205649 |
| the occurrence of beta-amylase in entamoeba histolytica. | a beta-amylase like activity isolated from a cell homogenate of entamoeba histolytica, and separated from other glucohydrolytic activities by gel filtration and isoelectric focusing, hydrolyzed amylose, amylopectin and glycogen, yielding maltose as reaction product. from the non-reducing ends of the 4-nitrophenyl-alpha-d-glycosides of maltotriose, maltotetraose, maltopentaose and maltohexaose the enzyme removed maltose units. the ph optimum for hydrolyzing the poly- and oligosaccharides was ph 6 ... | 1984 | 6205266 |
| increased leucocyte histamine release by entamoeba histolytica antigen in patients with amoebic abscess of the liver. | leucocytes (basophils) from non-atopic adult subjects living in an area highly endemic with entamoeba histolytica release histamine in a dose-dependent fashion upon in vitro exposure to an antigen of axenically grown e. histolytica (histolyticin). leucocytes of patients with acute amoebic liver abscess were significantly more sensitive to this antigen than leucocytes of control subjects, including patients that had recovered from amoebic liver abscess. by comparison concanavalin-a induced histam ... | 1984 | 6204266 |
| anti-ia reactivity in sera from subjects with entamoeba histolytica infection. | sera from 15 patients with entamoeba histolytica infection were tested for anti t-cell antibodies by assessing cross-reacting specificities with the antigens defined by an anti-ia hybridoma antibody. t cells prepared by sheep erythrocyte rosetting were preincubated with the test sera and then with the anti-ia antibody. binding of the specific monoclonal antibody was assessed by rosetting with ox erythrocytes conjugated with goat anti-mouse igg. eight sera from amoebic patients were found to bloc ... | 1984 | 6200968 |
| a fluorescent technique for demonstrating the chromatoid bodies and nuclei in cysts of entamoeba histolytica from faecal deposits. | 1984 | 6200503 | |
| [possible participation of bacteria in the formation of surface-active centers on the trophozoite of the dysentery ameba]. | 1983 | 6195515 | |
| [diarrhea caused by parasites]. | 1982 | 6191465 | |
| haemolysis: an in vitro model to investigate tissue necrotic activity of entamoeba histolytica. | 1982 | 6186601 | |
| [in vitro liberation of leukocyte histamine caused by amebic antigen and by concanavalin in patients with invasive amebiasis]. | 1982 | 6185099 | |
| entamoeba histolytica: ultrastructural localization of ca2+-dependent nucleotidases. | 1982 | 6182018 | |
| a pathway for the interconversion of hexose and pentose in the parasitic amoeba entamoeba histolytica. | isotope studies indicate that hexose-to-pentose interconversion by axenic entamoeba histolytica conserves the c-1 and c-6 hexose carbon atoms. transketolase was readily identified in amoebal extracts, and transaldolase could not be demonstrated. however, sedoheptulose 7-phosphate is a substrate for the ppi-dependent amoebal phosphofructokinase, and sedoheptulose 1,7-bisphosphate is cleaved by amoebal aldolase to dihydroxyacetone phosphate and erythrose phosphate. since these three enzymes cataly ... | 1982 | 6180735 |
| [probable antigenic relation between entamoeba histolytica and alpha-fetoprotein]. | the findings of alpha fetoprotein antigens are reported in raw extracts of entamoeba histolytica and also, on the membranes of trophozoites obtained from an axenic culture medium. this was confirmed through two techniques: ouchterlony's immunodiffusion and indirect immunofluorescence using an antialpha fetoprotein rabbit antiserum. previously, it was shown that this last antiserum did not recognize antigens of bovine albumin. the presence of alpha fetoprotein antigens in e. histolytica suggest t ... | 1982 | 6178418 |
| use of indomethacin to demonstrate enterotoxic activity in extracts of entamoeba histolytica trophozoites. | the present study was designed to develop and characterize animal models for the assay of enterotoxic activity in extracts of entamoeba histolytica trophozoites. marked water and electrolyte secretion occurred in both in vivo rabbit ileal loops and rat colon loops exposed to clarified sonic fluids of e. histolytica strain hm-1 trophozoites (10(6)/ml) when the animals were first administered indomethacin (0.1 mg/kg). no effect on intestinal absorption was observed in animals exposed to entamoeba ... | 1982 | 6177638 |
| gel filtration pattern of axenic entamoeba histolytica and characterization of immunological specificities of various fractions. | 1982 | 6177631 | |
| [histochemical aspects of liver lesions induced in hamsters by inoculation of e. histolytica in axenic culture. ii. evidence of perinecrotic fibrosis]. | the percutaneous, intrahepatic inoculation of e. histolytica trophozoites in weanling hamsters produced lesions of various shapes and sizes. the time-span between inoculation and sacrifice did not correlate with the size of the lesions. histoenzymologic technics for acid and alkaline phosphatases, adenosinetriphosphatase (atpase) and glucose-6-phosphate dehydrogenase (g-6-p dh) showed that the necrotic material is positive to the two former phosphatases whereas atp-ase shows distorted bile canal ... | 1981 | 6172830 |
| inhibition of entamoeba histolytica cytotoxin by alpha 1 antiprotease and alpha 2 macroglobulin. | we previously reported partial purification of a proteinaceous substance with cytotoxic and enterotoxic activity isolated from the soluble fraction of sonicated axenically cultivated entamoeba histolytica trophozoites. demonstration of cytotoxic activity of the preparation (amebal toxin) was dependent on removal of serum from the tissue culture assay system. the objective of the present study was to identify the factor(s) in non-immune sera responsible for producing in vitro inhibition of amebal ... | 1981 | 6167173 |
| concepts of function of peripheral non-chromatin and endosome in entamoeba histolytica. | the distribution of rna (to a lesser extent dna) in the nucleus of e. histolytica appeared to be the opposite of what one found in other eukaryotic cells (originally stated by pan and geiman in 1955). autoradiographs of amebae labelled with 3h-thymidine revealed primarily randomly distributed dna-3h (probably euchromatin) in interphase cells. this dna was so low in amount in individual cells that it usually did not stain with the feulgen procedure. a small amount of dna-3h was also detected by a ... | 1980 | 6162429 |
| [staining of cysts of entamoeba invadens, entamoeba histolytica and entamoeba coli with wheat germ agglutinin labelled with colloidal gold]. | cysts of entamoeba invadens obtained under axenic conditions were stained with wheat germ agglutinin labelled with colloidal gold. microscopic observation of encysting cultures revealed that upon staining, cysts acquired a red coloration, while trophozoites remained unstained. e. histolytica and e. coli cysts obtained from asymptomatic patients were also stained red by this technique. electronmicroscopic examination of stained cells showed a layer of colloidal gold granules attached to the surfa ... | 1980 | 6162428 |
| purification of the antigen specific for amoebiasis. | two methods used for the purification of specific antigen for amoebiasis were affinity chromatography and agarose gel electrophoresis. the igg fraction of the serum from a patient with amoebic liver abscess showing a specific band in the iep test was coupled to cnbr-activated sepharose 4b. after passage through the immunoadsorbent column of the crude or partially purified extract of entamoeba histolytica, followed by acid dissociation, specific antigen was obtained. this antigen gave only one ba ... | 1980 | 6160623 |
| antigenic variations among strains of entamoeba histolytica. | 1980 | 6156662 | |
| parasite-reactive serum ige antibodies in african populations. relation to intestinal parasite load. | serum ige levels and ige antibodies against four intestinal parasites, and the presence and abundance of parasites in stool samples were investigated in 161 rwanda natives. most of the ige turn out to be helminth-reacting antibodies. the stimulation and production of these antibodies are much different from those of anti-amoeba antibodies in their relation to the specific intestinal parasite load; helminth infestations seem to play a major role in the development of anti-amoeba antibodies as wel ... | 1980 | 6154649 |
| entamoeba histolytica "toxin": fetuin neutralizable and lectin-like. | extracts of entamoeba histolytica produce rounding and agglutination of mammalian cells in tissue culture. this effect is inhibited or reversed by serum, by the serum glycoprotein, fetuin, or by n-acetyl-d-galactosamine. concanavalin a produces a similar cytotoxic effect that is blocked or reversed by alpha-methyl mannoside. these properties suggest that the amebal "toxin" has a lectin-like activity. | 1980 | 6153255 |
| comparison of conventional and immunofluorescent techniques for the detection of entamoeba histolytica in rectal biopsies. | two comparative studies of six staining techniques in detection of entamoeba histolytica in rectal biopsies were performed. the techniques evaluated were direct and indirect fluorescent antibody technique, and four conventional stains: pas, h & e, trichrome, and ptah). on ethanol-fixed tissue, the direct fluorescent antibody technique was the most sensitive, detecting amoebas in 84% of rectal biopsies from 19 aspirate-positive dysentery patients. pas staining was specific, almost as sensitive, a ... | 1980 | 6153164 |
| cytopathogenicity of entamoeba histolytica. | the lesions induced in man by entamoeba histolytica are characterized by massive tissue injury in the absence of major local signs of a host immune response. the amoeba damages surrounding cells preferentially by contact-mediated cytolysis. recently, a presumptive aetiological factor underlying this process has been identified. it is a protein, amoebapore, capable of spontaneous incorporation into host cell membranes. therein it induces high conductance ion-channels which rapidly collapse the ce ... | 1984 | 6151690 |
| viruses and the pathogenicity of entamoeba histolytica. | 1984 | 6148562 | |
| viruses and pathogenicity of entamoeba histolytica. | 1984 | 6147594 | |
| entamoeba histolytica cyst passers: clinical features and outcome in untreated subjects. | to examine whether entamoeba histolytica is the cause of a variety of intestinal disorders, as often implicated, a two-part study was conducted. in part 1, 184 patients attending a gastroenterology clinic and needing a sigmoidoscopy had a rectal biopsy specimen and a stool sample cultured for e histolytica. part of the biopsy specimen was examined histologically. in addition, serum was tested for antibody to e histolytica. 34 (18.7%) patients were culture positive. there was no correlation betwe ... | 1984 | 6146859 |
| selective primary health care: strategies for control of disease in the developing world. x. amebiasis. | the human parasite entamoeba histolytica has a worldwide distribution. the majority of infected individuals suffer few symptoms; nonetheless, a variable proportion experience dysentery, hepatic abscesses, or invasion of other extraintestinal organs. invasive amebiasis is a major health problem, particularly in certain developing countries. the aim of this review is to clarify, as much as possible, controversial issues about the biology of the parasite, the clinical features of amebiasis, and the ... | 1983 | 6140740 |
| susceptibility testing of entamoeba histolytica. | the growth of entamoeba histolytica in microtiter plates in vitro in a variety of environments with reduced oxygen tensions is reported. with 3% o2, 3% co2, and 94% n2, the parasite growth in microtiter plates was identical to that in screw-capped culture tubes, as measured by [3h]thymidine incorporation and by quantitative parasite counts. there were no significant differences between the drug concentrations necessary to inhibit parasite growth by 50% based on [3h]thymidine incorporation vs tho ... | 1983 | 6140291 |
| biochemical homogeneity of entamoeba histolytica isolates, especially those from liver abscess. | the electrophoretic mobility patterns of four enzymes from trophozoites of entamoeba histolytica were examined to determine the zymodeme of the amoebae populations involved. e. histolytica populations from aspirated liver pus belonged exclusively to types ii and xi of eighteen zymodemes. e. histolytica from cases of amoebic dysentery--i.e., those patients presenting with intestinal ulceration and/or with haematophagous trophozoites in the faeces--also belonged to zymodemes ii and xi. however, sy ... | 1982 | 6123680 |
| double infection with entamoeba histolytica and echinococcus. | 1982 | 6122933 | |
| sexual transmission of enteric pathogens. | 1982 | 6120367 | |
| use of bisacodyl suppository in the diagnosis of amoebiasis. | 1982 | 6119453 | |
| [health of school children on the ivory coast. parasitology--serologic survey]. | 1980 | 6111909 | |
| [diagnosis of intestinal amebiasis]. | three cases of intestinal amebiasis with varying clinical pictures are presented. one patient developed an amebic liver abscess and also amebic colitis, which was mistaken for crohn's disease. a second patient with amebic dysentery had no entamoeba histolytica trophozoites in fecal material because of previous therapy with an antiprotozoal drug. the third patient, who had colitis and severe constitutional symptoms, had trophozoites in the stool but negative serological tests. the diagnostic valu ... | 1981 | 6111842 |
| adhesion of pathogenic microorganisms. | 1980 | 6105620 | |
| bruceantin, a potent amoebicide from a plant, brucea antidysenterica. | bruceantin, purified from an ethiopian plant used to treat dysentery, killed entamoeba histolytica in vitro (ic50 [the concentration of drug which decreased the number of colonies to half that of controls] = 0.018 microgram/ml). six related quassinoids of 17 tested were also amoebicidal. no relationship between quassinoid structure and amoebicidal activity was apparent. | 1982 | 6100431 |
| immuno-diagnosis of amoebiasis by antibody detection. | 1984 | 6100369 | |
| [on a case of autochthonous amebiasis]. | amoebiasis is usually acquired in countries where the disease is endemic. a case is presented of hepatic infection acquired indigenously. the authors emphasized the importance of amoebic hepatic abscess as potential cause for delay in diagnosis. | 1982 | 6100359 |
| immunochemical characterization of entamoeba histolytica plasma membrane's own and culture medium antigens. | 1984 | 6100162 | |
| a comparison of iha and elisa tests in the detection of antibodies to entamoeba histolytica. | in this report we have evaluated the sensitivity and specificity of iha and elisa for the detection of anti-amebic antibodies in the serum samples of 147 patients with poor mental and hygienic status hospitalized in mental institutions. our data have demonstrated a positive agreement between elisa and iha on positive sera. the major drawback of iha is the occurrence of false negative results, 13/82 sera negative in iha (less than 1/128) are positive in the elisa. furthermore, the elisa is useful ... | 1984 | 6100069 |
| amoebicidal activity of the antifungal antibiotic sinefungin against entamoeba histolytica. | the antifungal antibiotic sinefungin, a potent inhibitor of methyl transferases and a potential inhibitor of polyamine biosynthesis, exhibited marked cytolytic activity against entamoeba histolytica in vitro with respect to all the amoeba strains tested. strains of high and low virulence displayed equal sensitivity to the antibiotic. minimal amoebicidal concentration was 5 to 10 micrograms/ml. | 1984 | 6099924 |
| antibody-dependent lymphocyte mediated cytotoxicity in amoebiasis. | 1984 | 6099823 | |
| [histochemical study of entamoeba histolytica (schaudinn, 1903) obtained from the intestines of various hosts and histochemical and histological analysis of the intestines of infected animals]. | 1984 | 6099635 | |
| [ultrastructural study of acid phosphatase localization in entamoeba histolytica trophozoites and the accompanying bacteria of the intestinal flora]. | 1984 | 6099461 | |
| enzyme-linked immunosorbent assay in amoebiasis. | 1984 | 6099332 | |
| antibiotics in the chemotherapy of amebiasis. | amoebiasis is a widespread disease with high prevalence in tropical countries and environments with poor sanitation. the treatment of either intestinal and extra-intestinal localization of entamoeba histolytica or encephalic lesions sustained by acanthamoeba and naegleria has been the object of a wealth of research. antibiotics played a dominant role in this field. their antiamebic activity is due either to direct action on the protozoan and/or inhibition of symbiotic bacterial flora. presently ... | 1984 | 6099226 |
| [coproparasitological diagnosis by modified telemann's and burrows' methods]. | 1984 | 6098283 | |
| syntheses and biological activities of 1,4-disubstituted piperidines. | 1984 | 6098241 | |
| entamoeba histolytica: antibody responses and lymphoreticular changes in gerbils (meriones unguiculatus) in response to experimental liver abscess and amebic extract infection. | antibody responses and histological changes in hepatic lymph nodes and spleen of gerbils (meriones unguiculatus) during the course of experimental hepatic amebiasis (5-60 days), or in those injected with extracts of entamoeba histolytica, are described. lymph node and spleen responses in infected animals paralleled the proliferation of the amebic liver abscess. however, spleen follicle responses were similar in animals that received low or high doses of the amebic extract and differed histologic ... | 1984 | 6098090 |
| hepatic amebiasis. | 1984 | 6098014 | |
| effect of lowering the peripheral level of 5-hydroxytryptamine in host on the virulence of entamoeba histolytica. | 1984 | 6097538 | |
| [the size of entamoeba histolytica, entamoeba coli and giardia lamblia cysts]. | 1984 | 6097375 | |
| [hepatic amebiasis with perforation of the pericardium]. | 1984 | 6097284 | |
| thiol groups on the surface of anaerobic parasitic protozoa. | evidence is presented that giardia lamblia and entamoeba histolytica, phylogenetically unrelated aerotolerant anaerobes, have crucial thiol groups on or easily accessible to their external surface. both parasites were killed by three structurally unrelated thiol-blocking reagents which penetrate intact cells poorly or not at all. the parasites were protected from p-chloromercuribenzenesulfonic acid (10-100 microm) by cysteine or by reduced glutathione. killing was arrested with identical kinetic ... | 1984 | 6096710 |
| gastric amebiasis in a silvered leaf monkey. | 1984 | 6096330 | |
| amoebiasis in homosexual men. | 1984 | 6096229 | |
| caerulomycin, an antifungal antibiotic with marked in vitro and in vivo activity against entamoeba histolytica. | the anti-amoebic action of the bipyridyl antibiotic caerulomycin was assessed in vitro and in vivo using various strains of entamoeba histolytica from polyxenic, axenic and monoxenic cultures. minimum inhibition concentrations of caerulomycin (metronidazole) were 7.5 (5), 15.6(1.95) and 60 (2.5) micrograms/ml against polyxenic, axenic and monoxenic cultures of e. histolytica, respectively. the ed50 values ascertained in golden hamsters (extraintestinal amoebiasis) and rats (intestinal amoebiasis ... | 1984 | 6095553 |