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novel genes that upregulate the proteus mirabilis flhdc master operon controlling flagellar biogenesis and swarming.by screening for restoration of multicellular migration in a non-swarming but motile proteus mirabilis mutant lacking the fign facilitator of flagella assembly, we identified four distinct genes that, in trans and multicopy, increased flagella production and cell length. each of the genes upregulated expression of the flhdc master operon that controls flagellar biogenesis, cell division and swarming, not only in the mutant but also in the wild type. the genes were named umoa, umob, umoc and umod ...19989723914
a rapid method for determination of in vitro susceptibility to antibiotics with a bulk acoustic wave bacterial growth biosensor.a novel bulk acoustic wave (baw) bacterial growth biosensor was developed to study in vitro susceptibility by continuous monitoring of disturbances of bacterial growth at low antibiotic concentrations, followed by the accurate and rapid estimation of growth kinetic parameters and minimum inhibitory concentrations (mics). the susceptibilities of bacteria, e.g. escherichia coli, staphylococcus aureus, proteus vulgaris, pr. morganii and pr. mirabilis, to various antibiotics, e.g. penicillin, strept ...19989722998
a new bacteriophage typing scheme for proteus mirabilis and proteus vulgaris strains. 3. analysis of lytic properties.the lytic properties of 21 bacteriophages constituting a new typing set for proteus were examined in 507 proteus mirabilis and 29 p. vulgaris strains isolated from patients and healthy subjects. comparison of their morphological, serological, genetic and lytic properties showed that, in the myoviridae and podoviridae families, some phages were so closely related that the presence of all of them in the set was redundant. analysis of the lytic properties revealed that some of the bacteriophages we ...19989721605
asymmetric reduction of racemic sulfoxides by dimethyl sulfoxide reductases from rhodobacter capsulatus, escherichia coli and proteus species.the enantioselective reduction of racemic sulfoxides by dimethyl sulfoxide reductases from rhodobacter capsulatus, escherichia coli, proteus mirabilis and proteus vulgaris was investigated. purified dimethyl sulfoxide reductase from rhodobacter capsulatus catalysed the selective removal of (s)-methyl p-tolyl sulfoxide from a racemic mixture of methyl p-tolyl sulfoxide and resulted in an 88% recovery of enantiomerically pure (r)-methyl p-tolyl sulfoxide. rhodobacter capsulatus was shown to be abl ...19989720047
utilization of proteinase k-treated cells as lipopolysaccharide antigens for the serodiagnosis of helicobacter pylori infections.we have evaluated the use of proteinase k (pk)-treated cells isolated from helicobacter pylori as lipopolysaccharide (lps) antigens in an immunoblot assay and an enzyme-linked immunosorbent assay (elisa) for the serodiagnosis of h. pylori infection. the sera from patients with chronic gastritis, gastric ulcer, duodenal ulcer or gastric cancer, and from healthy adults with or without h. pylori infection were assayed with three commercial serodiagnostic kits (hm-cap, helico-g, and g.a.p. ii) and n ...19989719104
evidence that aminoguanidine inhibits endotoxin-induced bacterial translocation.the role of inducible nitric oxide synthase (inos) in endotoxin-induced bacterial translocation was investigated by using its specific blocker aminoguanidine in 46 albino mice (25-35 g) allocated into four groups.19989718006
lyme borreliosis.lyme borreliosis (lyme disease) is often said to be associated with "protean" manifestations, a reference to the ancient god proteus, who could assume many forms and thus elude his pursuers. this legendary quality has clouded our understanding of lyme borreliosis by giving borrelia burgdorferi infection a mythical aura of its own. this review shows that this illness, while incompletely understood, is far more palpable than proteus and is (in most cases) much more readily subdued. the clinical pr ...19989716075
characterization of a monoclonal antibody and a cdna for polyubiquitin of amoeba proteus.a monoclonal antibody was obtained that reacts with many different proteins (14-200 kda) of amoeba proteus. by indirect immunofluorescence microscopy we found the antigens to be dispersed throughout the cytoplasm but were more concentrated in the nucleus. the antibody cross-reacted with proteins of tetrahymena, xenopus embryo, and mouse macrophages. using the antibody as a probe we cloned a cdna of 1.2 kb coding for ubiquitin in five repeats. amino acid sequences of ameba's polyubiquitin showed ...19989703679
which bacteria are found in belgian women with uncomplicated urinary tract infections in primary health care, and what is their susceptibility pattern anno 95-96?dysuria is a frequent reason for encounter in general practice and also gynaecologists and urologists will be frequently confronted with it. in female patients 1/2 to 2/3 of dysuric episodes are due to urinary tract infections. in nearly all cases therapy is started before the results of a culture is available. therefore it is very important to know which bacteria are most prevalent in the treated population, and what their susceptibility pattern is . because most available information is based ...19989701853
effects of prophylactic antibiotics on endometrial flora in women with postcesarean endometritis.to determine the effect of prophylactic antibiotics on endometrial and endocervical microflora upon diagnosis of postcesarean endometritis.19989699764
encrustation of indwelling urethral catheters by proteus mirabilis biofilms growing in human urine.the encrustation and blockage of four types of urinary catheters was studied in a simple laboratory model of the catheterized bladder. pooled human urine was supplied to the bladder chamber at 0.5 ml/min. the bladder urine was inoculated with a clinical strain of proteus mirabilis that had been isolated from an encrusted catheter. the models were operated until the catheters blocked and atomic absorption spectrometry was used to assess the amounts of calcium and magnesium deposited on the cathet ...19989699143
a swarming-defective mutant of proteus mirabilis lacking a putative cation-transporting membrane p-type atpase.the motile tnphoa mutant ic24 of proteus mirabilis u6450 generates an aberrant swarming colony, and was shown to be impaired in swarm cell differentiation, i.e. cell elongation and hyperflagellation, causing delayed and slower population migration across a solid growth medium. levels of transcript from the flagellin filament gene flic, the flagellar master operon flhdc, and the leucine-responsive regulatory protein gene lrp, a regulator of swarming differentiation, were reduced in ic24 mutant sw ...19989695928
identification of a novel transcription regulator from proteus mirabilis, pmtr, revealed a possible role of yjai protein in balancing zinc in escherichia coli.zinc is an essential trace element required for structural integrity and functional activity of numerous proteins, yet mechanisms by which cells regulate zinc concentration are poorly understood. here, we identified a gene from proteus mirabilis that encodes a 135-amino acid residue protein, pmtr (p. mirabilis transcription regulator), a new member of the merr family of transcription activators. transformation of escherichia coli with pmtr-carrying vectors specifically increases cell tolerance t ...19989694902
bulk acoustic wave bacterial growth sensor applied to analysis of antimicrobial properties of tea.a bulk acoustic wave (baw) bacterial growth sensor has been proposed for study of inhibitory effects of tea by continuous monitoring of disturbances in proteus growth in the aqueous extracts of various teas, e.g. green tea, fuzhuan brick tea, oolong tea, kudin tea, and black tea. the kinetic parameters, e.g. asymptote (a), maximum specific growth rate (microm), lag time (lambda), and generation time (g), accurately estimated by using the growth response model, have been first used to characteriz ...19989694687
terpenoid composition of the wound-induced bark exudate of commiphora tenuis from ethiopia.the bark of commiphora tenuis vollensen exudes a translucent, free-flowing odoriferous liquid upon wounding which was analysed by capillary glc and glc-ms. 42 mono- and sesquiterpenes were detected and 37 identified. the main components of the monoterpenoid fraction were alpha-pinene (60.8%), beta-pinene (8.8%), sabinene (6.3%), alpha-thujene (8.9%), limonene (5.5%), 3-carene (3.7%), beta-myrcene (1.8%), and beta-elemene (1.1%) constituting 97% of the oil. identified sesquiterpenoid components c ...19989690355
[the effect of gentamycin on immunity in immunodeficiency and the action of immunomodulators].the effect of 3 and 6 mg/kg gentamicin on the immunity of intact mice and cyclophosphan- and azathioprine-induced immunodeficiency was studied. gentamicin deepens the severe suppression of antibody genesis against the background of cyclophosphan and azathioprine, inhibits the reaction of gzt in intact mice. the antibiotic does not prevent stimulation of aok production by methyluracil and prodigiosan, and does not change the effect of immunostimulators on gzt in immunosuppression. gentamicin has ...19989690079
psoas abscess following extracorporeal shock wave lithotripsy.different studies have reported renal effects like swelling of parenchyma, subcapsular and intracapsular haematoma and perinephric fluid collections. these effects were usually transient and by 2-3 months returned to normal. we report an early complication of extracorporeal shock wave lithotripsy (eswl). a patient with solitary renal calculus who had eswl developed psoas spasm and became unwell with elevation of white cell count and erythrocyte sedimentation rate. psoas abscess extending from re ...19989689709
pathology of lipomatous lesions in proteus syndrome.proteus syndrome is an extremely rare, complex hamartomatous disorder with markedly variable clinical expression. we present a case of proteus syndrome with multiple disfiguring soft tissue masses that were present since early childhood. the lesions involved predominantly the right side of the body and included scoliosis, macrodactyly, and limited hyperostosis in the right foot. there was no evidence of cranial or skin lesions. the patient underwent multiple resections of soft tissue masses, inc ...19989688770
bacteriology of burns.a retrospective study was undertaken at university college of medical sciences and guru teg bahadur hospital, delhi, to examine the bacterial isolates from the burns unit and to determine the antibiograms of the isolates to commonly used antimicrobial agents. a total of 600 pus samples from as many patients received, over a period of 5 years (june 1993-june 1997) yielded 920) isolates. pseudomonas spp. was the most common (36%) followed by staphylococcus aureus (19%), klebsiella spp. (15.54%), p ...19989688200
molecular characterization of a tem-21 beta-lactamase in a clinical isolate of morganella morganii.a clinical isolate of morganella morganii, with reduced susceptibility to expanded-spectrum cephalosporins and aztreonam, was found to produce an extended-spectrum beta-lactamase with a pi of 6.4. the nucleotide sequence of the encoding gene was that of the gene encoding tem-21. this is the first molecular characterization of an extended-spectrum beta-lactamase in m. morganii.19989687421
cefepine vs. ceftazidime treatment of pyelonephritis: a european, randomized, controlled study of 300 pediatric cases. european society for paediatric infectious diseases (espid) pyelonephritis study group.cefepime has been used in clinical therapeutic trials for meningitis, serious infection and febrile neutropenia, comprising more than 800 pediatric patients. this agent has also been used in patients 12 years of age and older with uncomplicated and complicated urinary tract infections including pyelonephritis, but not in younger patients. in this study the safety and efficacy of cefepime were compared with those of ceftazidime for treatment of pyelonephritis in pediatric patients younger than 12 ...19989686732
3,6-diamino-10-methylacridan: uncharged precursor of acriflavine and its unique antimicrobial activity. 19989685231
mosaicism for a tandem duplication dup(1)(q12q22) in an 18 year old female.the clinical features and cytogenetic results of an 18 year old mentally handicapped female found to be a mosaic for a tandem duplication of chromosome 1 (46,xx,dup(1)(q12q22)/46,xx) are reported. the case is compared with the three previously described cases and possible mechanisms for the origin of the duplication are discussed. this patient was not found to have features of proteus syndrome which was previously reported in a subject mosaic for a tandem duplication involving chromosome (1)(q11 ...19989678707
intravascular ultrasound diagnosis of aortic graft infection.to assess the value of intra-aortic ultrasound (us) for diagnosing intraprosthetic vegetations in atypical aortic graft infection.19989678551
tc-99m hmpao leukocyte and tc-99m nanocolloid scintigraphy in posttraumatic bone infection.posttraumatic osteomyelitis was investigated in 23 patients using nuclear medicine techniques. tc-99m hexamethylpropilene amine oxime (hmpao)-labeled leukocyte scintigraphy was performed in 11 patients, and tc-99m nanocolloid scanning was performed in the other 12 patients. the scintigraphic findings were compared with clinical, laboratory, radiologic, and bacteriologic results. the findings on leukocyte imaging were consistent with the clinical symptoms in 7 of 11 patients, with the laboratory ...19989676945
cloning and characterisation of the proteus mirabilis xerd gene.the xer site-specific recombination system is involved in the stable maintenance of replicons (certain plasmids and chromosomes) in escherichia coli and other bacteria by converting multimers into monomers. this system requires a cis-acting dna sequence (the chromosomal dif site or the cole1 cer site) and two trans-acting factors: the xerc and xerd recombinases, which belong to the lambda integrase family of tyrosine site-specific recombinases. in addition, in order to resolve plasmid multimers ...19989675854
recurrent bilateral renal calculi in a tetraplegic patient.an 18-year-old male developed c-5 complete tetraplegia following a motor-cycle accident in may 1975. the neuropathic bladder was managed by an indwelling urethral catheter. he developed recurrent episodes of urinary infection with proteus species. in september 1975, an x-ray of the abdomen revealed small calculi in both the kidneys. in july 1976, he underwent transurethral resection of the bladder neck and division of the external urethral sphincter; subsequently, he was put on a penile sheath d ...19989670380
further studies of transferable antibiotic resistance in strains of pseudomonas aeruginosa from four clinical settings in three countries.this paper describes transferability of antibiotic resistance determinants in clinical isolates of pseudomonas aeruginosa resistant to imipenem, cefotaxime and ceftazidime obtained from different clinical settings in three different countries. two strains of enterobacteriaceae (escherichia coli k-12 and proteus mirabilis p-38) and two strains of p. aeruginosa (pao and ml) were used as recipient strains. the conjugative transfer of resistance was very specific, i.e. donor strains of p. aeruginosa ...19989669646
bacterial adherence to self-reinforced polyglycolic acid and self-reinforced polylactic acid 96 urological spiral stents in vitro.the aim of this study was to evaluate the bacterial adherence to biodegradable self-reinforced polyglycolic acid (sr-pga) and self-reinforced poly-dl-lactic acid (sr-pla 96) spiral stents in vitro. they are used as temporary urethral stents in urology. gold-plated metal wire, polyurethane and latex were used as controls. materials were incubated up to 28 days in artificial urine, after which a bacterial suspension was added. after detaching by sonication the adhesive bacteria were analysed as co ...19989663740
management of animal bite injuries of the face: experience with 94 patients.because of the large number of bacteria in the oral cavity, animal bite wounds are generally contaminated, and their treatment is difficult because of the risk of infection, especially in extensive injuries. this report describes the management of a large series of patients and recommends treatment guidelines on the basis of the results.19989663574
urinary excretion and bactericidal activities of a single oral dose of 400 milligrams of fleroxacin versus a single oral dose of 800 milligrams of pefloxacin in healthy volunteers.twelve healthy volunteers participated in this randomized crossover study to compare the concentrations and recovery levels of fleroxacin and pefloxacin in urine and to assess their bactericidal activities against 12 strains of urinary pathogens with different susceptibilities over a wide range of mics. the volunteers received a single oral dose of 400 mg of fleroxacin or 800 mg of pefloxacin. the mean cumulative renal excretion of unchanged fleroxacin, n-demethyl-fleroxacin, and n-oxide-fleroxa ...19989661000
structure of the o-specific polysaccharide of proteus penneri strain 41 from a new proposed serogroup o62.o-specific polysaccharide of proteus penneri strain 41 was studied using 1h- and 13c-nmr spectroscopy, including two-dimensional cosy, heteronuclear 13c,1h-correlation (hetcor) and one-dimensional noe spectroscopy, and the following structure of a non-stoichiometrically o-acetylated hexasaccharide repeating unit was established:[structure: see text] where rglcnac is 2-acetamido-4-o-[(s)-1-carboxyethyl]-2-deoxyglucose. cross-reactivity of anti-p. penneri 41 o-serum with other p. penneri strains i ...19989657315
a mixed disulfide bond in bacterial glutathione transferase: functional and evolutionary implications.glutathione s-transferases (gsts) are a multifunctional group of enzymes, widely distributed in aerobic organisms, that have a critical role in the cellular detoxification process. unlike their mammalian counterparts, bacterial gsts often catalyze quite specific reactions, suggesting that their roles in bacteria might be different. the gst from proteus mirabilis (pmgst b1-1) is known to bind certain antibiotics tightly and reduce the antimicrobial activity of beta-lactam drugs. hence, bacterial ...19989655824
structure and cross-reactivity of the o-specific polysaccharide of proteus penneri strain 26, another neutral proteus o-antigen containing 2-acetamido-2,6-dideoxy-l-glucose (n-acetyl-l-quinovosamine).a neutral o-specific polysaccharide obtained from the lipopolysaccharide of proteus penneri strain 26 was studied using sugar analysis and 1h and 13c nmr spectroscopy, including two-dimensional nmr techniques. the following structure of the trisaccharide repeating unit was established: -->6)-alpha-d-glcpnac-(1-->3)-alpha-l-quipnac-(1-->3)-alpha-d-glcp nac-(1--> where l-quinac is 2-acetamido-2,6-dideoxy-l-glucose (n-acetyl-l-quinovosamine). cross-reactivity of the proteus penneri 26 anti-o serum ...19989654072
the efficiency of different phenol-degrading bacteria and activated sludges in detoxification of phenolic leachates.phenolic composition, toxicity and biodegradability of three different phenolic leachates/samples was studied. samples a and c were the leachates from the oil-shale industry spent shale dumps at kohtla-järve, estonia. sample b was a laboratory-prepared synthetic mixture of 7 phenolic compounds mimmicking the phenolic composition of the leachate a. toxicity of these 3 samples was analyzed using two photobacterial test (biotox and microtox), daphnia test (daphtoxkit f pulex) and rotifiers' test (r ...19989650267
escherichia coli and proteus mirabilis inhibit the perinuclear but not the circulating antineutrophil cytoplasmic antibody reaction.perinuclear antineutrophil cytoplasmic antibodies (p-anca) are found in 48%-83% of serum samples from patients with ulcerative colitis (uc). their pathogenic role and initiating stimuli are unknown. in contrast to patients with vasculitides and anca reactivities, the antibodies in uc patients do not react with myeloperoxidase (mpo) or proteinase 3 (pr3). the aim of the present study was to investigate whether bacterial species of the intestinal tract and other sources could interfere with p-anca ...19989648994
gram-negative bacteremia in non-neutropenic patients: a 3-year review.the causative organisms, clinical manifestations, factors influencing prognosis, and other epidemiological characteristics of 81 episodes of bacteremia due to gram-negative organisms, in non-neutropenic patients, were studied retrospectively during a 3-year period (1992-1994) at the department of internal medicine of the university hospital of heraklion, crete, greece. the gram-negative bacteremia incidence was 2% and the overall mortality 12%. all 81 patients had fever; escherichia coli was the ...19989646106
median nerve compression in proteus syndrome.proteus syndrome is a multi-organ disorder, a prime feature of which is localized gigantism, usually clinically obvious. symptoms secondary to hypertrophy of nerves has not been previously recognized as a part of the syndrome.19989639645
microbiologic evaluation of cutaneous wounds in hospitalized dermatology patients.the purpose of this study is to identify predictability of wound flora which may be helpful in the selection of empiric antibiotic therapy of wound infections while awaiting the results of bacterial cultures. toward this end, results of an ongoing microbiologic surveillance of skin wound flora in hospitalized dermatology patients are presented. bacterial isolates cultured from patients with skin wounds admitted to a tertiary care dermatology inpatient unit were evaluated and comparison was made ...19989626006
a comparison between single-dose fosfomycin trometamol (monuril) and a 5-day course of trimethoprim in the treatment of uncomplicated lower urinary tract infection in women.this study is a comparison of the microbiological and clinical efficacy of single-dose fosfomycin trometamol therapy and a 5 day course of trimethoprim in the treatment of uncomplicated urinary tract infection in female patients. urine dip-slide samples were obtained from 547 female patients aged 18-65 by 22 general practitioners (gps) participating in the study from 21 centres in the uk. all patients were diagnosed as having a urinary tract infection by their gp on the basis of history and clin ...19989624542
urinary bactericidal activity and pharmacokinetics of enoxacin versus norfloxacin and ciprofloxacin in healthy volunteers after a single oral dose.in an open, randomised monocentric crossover study in six male and six female healthy volunteers, the urinary antibacterial activity and pharmacokinetics of enoxacin, norfloxacin and ciprofloxacin were assessed. urine was collected up to 6 days, and venous blood samples up to 12 h, after a single oral dose of 400 mg enoxacin, 400 mg norfloxacin and 500 mg ciprofloxacin. enoxacin (250 mg/l) demonstrated the highest peak concentration (median) in the urine (0-6 h), followed by ciprofloxacin (237 m ...19989624541
beta-lactamases responsible for resistance to expanded-spectrum cephalosporins in klebsiella pneumoniae, escherichia coli, and proteus mirabilis isolates recovered in south africa.although resistance to the expanded-spectrum cephalosporins among members of the family enterobacteriaceae lacking inducible beta-lactamases occurs virtually worldwide, little is known about this problem among isolates recovered in south africa. isolates of klebsiella pneumoniae, escherichia coli, and proteus mirabilis resistant to expanded-spectrum cephalosporins recovered from patients in various parts of south africa over a 3-month period were investigated for extended-spectrum beta-lactamase ...19989624474
in vitro activity and clinical evaluation of cefixime in urinary tract infection.thirty-five women with uncomplicated acute lower urinary tract infections proven by significant pre-treatment bacteriuria (> or = 10(5)cfu/ml) were treated with an oral dose of 100 mg cefixime twice a day for seven days. thirty five patients included in this study were checked for response to treatment on the last day of therapy, 7-14 days and 4 weeks post therapy. the clinical response and bacterial eradication rate for cefixime were 91.4 per cent (32/35). the infecting organisms, e. coli and p ...19989623027
[nitazole--an antimicrobial substance].the antibacterial activity of protistocide drug nitazole has been revealed for the first time in the 80's at the laboratory of clinical microbiology of the mechnikov research institute of microbiology and immunology. unlike other imidazoles, such as metronidazole and tinidazole, nitazole acts as the inhibitor of growth of gram-positive facultative and obligate anaerobic microorganisms as well as gram-negatives except for pseudomonas aeruginosa and proteus. nitazole, as a main antimicrobial agent ...19989621693
[therapeutic effect of cefluprenam on polymicrobial urinary tract infection associated with enterococcus faecalis, using the infectious urolithiasis model in rats].we examined the therapeutic effect of cefluprenam (cflp) on the polymicrobial urinary tract infection associated with infectious stones as compared to that of ceftazidime (caz), using the experimental polymicrobial urinary tract infection caused by proteus mirabilis (p. mirabilis), pseudomonas aeruginosa (p. aeruginosa) and enterococcus faecalis (e. faecalis). in order to form bladder stones in rats, a sterile zinc ring was implanted as a foreign body, followed by inoculating p. mirabilis e05106 ...19989621566
[the immunotropic properties of rifampicin and their correction].the effect of rifampicin on antibody genesis depends on the dose, duration, and time of its administration in relation to the antigen, as well as on the character of immunosuppression. the antibiotic has no effect on delayed hypersensitivity and stimulates the activity of phagocytes, predominantly that of neutrophils. the effectiveness of rifampicin in experimental infection is maintained in treatment with cyclophosphane and azathioprine. when administered in combination with rifampicin and cyto ...19989621174
post-keratoplasty endophthalmitis caused by proteus mirabilis.a diabetic patient who underwent a triple procedure (penetrating keratoplasty, cataract extraction and posterior chamber intraocular lens implantation), developed endophthalmitis caused by proteus mirabilis. the source of infection was the infected donor cornea, which was imported from sri lanka. the organism was resistant to gentamicin, which was the only antibiotic present in the storage medium. on top of an aggressive antibiotic treatment regime (topical, systemic and intravitreal), exchange ...19989614531
antimicrobial activity of 20 plants used in folkloric medicine in the palestinian area.ethanolic and aqueous extracts of 20 palestinian plant species used in folk medicine were investigated for their antimicrobial activities against five bacterial species (staphylococcus aureus, escherichia coli, klebsiella pneumoniae, proteus vulgaris, pseudomonas aeruginosa) and one yeast (candida albicans). the plants showed 90% of antimicrobial activity, with significant difference in activity between the different plants. the most antimicrobially active plants were phagnalon rupestre and micr ...19989613839
the proteus syndrome: cns manifestations.proteus syndrome is a complex hamartomatous disorder characterized by multiple, diverse, somatic manifestations. we present a case in which severe, evolving cns abnormalities were also exhibited. imaging findings at presentation included hemimegalencephaly, subependymal calcified nodules, and periventricular cysts. subsequently, dural sinus thrombosis developed. eight previously reported patients may also have had hemimegalencephaly. when neuroimaging studies show hemimegalencephaly in a child w ...19989613526
antibacterial activity of peritoneal exudate in patients treated with 2 g cefotiam for surgical anti-microbial prophylaxis.the objective of this study was to investigate the presence of antibacterial activity in peritoneal exudate (pe) of patients treated with cefotiam (cft). cft (2 g) was administered as a 'single-shot' antimicrobial prophylaxis to 6 patients at the beginning of colorectal resection. samples of pe were collected from each patient on days 1, 2 and 3 after surgery. cft was detectable in the samples of day 1 for 5 of the 6 patients. the influence of pe on antibacterial activity of the antimicrobial dr ...19989612603
[improvement of effectiveness of antibiotic therapy by pyrimidine derivatives in reparative regeneration of skin in rats].it was shown on noninbred albino rats with various affections of the skin that pyrimidine derivatives stimulated the skin reparative regeneration and increased the efficacy of antibiotic therapy of staphylococcus and proteus infected wounds. the therapeutic effect of 2-methyl-4-amino-6-oxypyrimidine was much higher than that of oxymethyluracil or methyluracil. the pyrimidine derivatives proved to be universal accelerators for reparative regeneration, were compatible with antibiotics and increase ...19989606492
efficacy and safety of norfloxacin 800 mg once-daily versus norfloxacin 400 mg twice-daily in the treatment of uncomplicated urinary tract infections in women: a double-blind, randomized clinical trial.norfloxacin 400 mg twice-a-day has proven to be effective and safe in the treatment of uncomplicated urinary tract infections (uti). since previous pharmacokinetic studies demonstrate the feasibility of using norfloxacin 800 mg once-daily, and this scheme is assumed to improve patient compliance, a double-blind randomized clinical trial was performed to compare the efficacy and safety of norfloxacin 800 mg once-daily (group a) versus norfloxacin 400 mg twice-daily (group b) in the treatment of a ...19989603637
imipenem resistance in aerobic gram-negative bacteria.a prospective study was undertaken to observe the emergence of resistance to imipenem, if any, among aerobic gram-negative bacteria. a total of 736 isolates were tested during 1994-95 and less than 1% of them were resistant to imipenem, whereas the next year ('95-'96) the rate increased to 11 of the 903 isolates tested. the resistant isolates during '94-'95 were all stenotrophomonas maltophilia whereas the spectrum of resistant bacterial species increased in '95-'96 to include pseudomonas aerugi ...19989603633
gigantism of the foot: our experience in seven cases.we report our experience in seven patients with congenital gigantism of the foot with the following diagnoses: neurofibromatosis (two), fibrolipomatosis (two), proteus syndrome (two), and idiopathic localized gigantism (one). our purpose is to introduce a new classification of foot gigantism, based on the concept of "neuroinduction." in our experience, intraoperative examination and subsequent histologic examination show consistently pathologic findings in the plantar nerve and its terminal bran ...19989600560
pediatric peripheral neuropathy in proteus syndrome.proteus syndrome is a rare congenital disorder comprised of subcutaneous and internal hamartomas, pigmented skin nevi, skull exostoses, hemihypertrophy, and macrodactyly of the hands and feet. a 5-year-old girl diagnosed with proteus syndrome presented with distal median compression neuropathy with the primary complaint of severe pain involving the left hand. surgical exploration of the hand revealed a lipofibromatous hamartoma of the median nerve. the transverse carpal ligament was released and ...19989600441
an in-vitro study of carbapenem-induced morphological changes and endotoxin release in clinical isolates of gram-negative bacilli.one hundred clinical isolates, including escherichia coli, serratia marcescens, klebsiella pneumoniae, pseudomonas aeruginosa, proteus vulgaris and proteus mirabilis, were exposed to carbapenems (imipenem, panipenem, meropenem and biapenem) at 0.5 x mic for 3 h, then their morphology was examined and endotoxin release determined. ceftazidime, which induces filament formation, was used as a control. scanning electron microscopy showed that these carbapenems induced formation of spherical or ovoid ...19989598774
cytochalasin-induced actin disruption of polarized enterocytes can augment internalization of bacteria.cytochalasin-induced actin disruption has often been associated with decreased bacterial internalization by cultured epithelial cells, although polarized enterocytes have not been systematically studied. in assays using confluent polarized ht-29 enterocytes, cytochalasin d appeared to increase internalization of wild-type salmonella typhimurium, proteus mirabilis, and escherichia coli. hela and hep-2 epithelial cells, as well as ht-29 and caco-2 enterocytes, were used to clarify this unexpected ...19989596696
interactions of beta-lactamases with sanfetrinem (gv 104326) compared to those with imipenem and with oral beta-lactams.sanfetrinem is a trinem beta-lactam which can be administered orally as a hexatil ester. we examined whether its beta-lactamase interactions resembled those of the available carbapenems, i.e., stable to ampc and extended-spectrum beta-lactamases but labile to class b and functional group 2f enzymes. the comparator drugs were imipenem, oral cephalosporins, and amoxicillin. mics were determined for beta-lactamase expression variants, and hydrolysis was examined directly with representative enzymes ...19989593145
chromosomally encoded ampc-type beta-lactamase in a clinical isolate of proteus mirabilis.a clinical strain of proteus mirabilis (cf09) isolated from urine specimens of a patient displayed resistance to amoxicillin (mic >4,096 microg/ml), ticarcillin (4,096 microg/ml), cefoxitin (64 microg/ml), cefotaxime (256 microg/ml), and ceftazidime (128 microg/ml) and required an elevated mic of aztreonam (4 microg/ml). clavulanic acid did not act synergistically with cephalosporins. two beta-lactamases with apparent pis of 5.6 and 9.0 were identified by isoelectric focusing on a gel. substrate ...19989593136
[the evaluation of cefmetazole in clinical use. the study group of cefmetazole in clinical use].to study the clinical effect of cefmetazole (cmz), we treated with cmz 1,926 patients with various infections involving the respiratory tract, gastrointestinal tract, liver and biliary tract, skin and soft tissue and others. the marked effect rate was 56.5%, and the effect rate 33.7%. the sensitivity test of cmz was also performed in vitro. staphylococcus aureus, streptococcus, staphylococcus epidermis, e. coli, s. typhi, b. salmonella, b. shigella, b. klebsiella pneumoniae, b. proteus, etc. wer ...19969592326
[fluticasone propionate, corticosteroid for topical treatment].the aim of this study was to evaluate the usefulness of fluticasone propionate in various dermatoses and to present the data to non-dermatologists. the study was performed at the department of dermatology, university school of medicine in poznań. the studied group consisted of 40 cases of various types of eczema, psoriasis vulgaris, dle, photodermatoses and morphea. fluticasone propionate cream or ointment was applied to lesional skin twice daily for 7-19 days. acute eczematous skin lesions sign ...19989591441
[the etiology of nosocomial infection in surgery: comparison of 2 years (1988 and 1996)].nosocomial infections (ni) make up an important problem in public health care. from an etiologic point of view they are characterized by their constant evolution over time. thus, the aim of this study was to know the etiologic variations of ni in the surgery departments of a university hospital.19989586365
[lithiasis in megacalyosis].to review our series of lithiasis associated with megacalyosis, a rare disease entity.19989586314
[selective screening of enterohemorrhagic escherichia coli depending on hemolysis on beutin's washed sheep blood agar plates].recently, beutin et al have studied hemolysin production in a large number of serologically diverse verotoxin (vt) producing escherichia coli (vtec) strains and found a close association between hemolysin production and vt production. this study was to examine whether hemolysin production on sheep blood agar can be used as an epidemiological marker for microbiological screening of enterohemorrhagic escherichia coli (ehec). in comparison of hemolysis on washed blood agar plates prepared in our la ...19989585695
aerobic and anaerobic bacteriology of otorrhea associated with tympanostomy tubes in children.the microbiology of in 55 ear aspirates obtained from 34 children with chronic otorrhea was studied. aspiration of the middle ear exudate was done immediately following removal of tympanostomy tube (tt). the middle ear aspirates and swab specimens of the external auditory canals were cultured for aerobic and anaerobic bacteria. sixty-five isolates were recovered only from the middle ears, 73 only from the external ear canals, and 73 were present at both sites. analysis of the 138 middle ear isol ...19989583788
effect of adherence on antimicrobial susceptibility of pseudomonas aeruginosa, serratia marcescens, and proteus mirabilis.a simple method was used for testing the antibiotic susceptibility of adherent bacteria to plastic surfaces. pseudomonas aeruginosa, serratia marcescens, and proteus mirabilis cells adhering to the bottom of a plastic tissue culture plate were incubated in serially diluted antibiotic solutions. after 24-h incubation the solutions were removed and a fresh medium without antibiotics was added to each well. the viability of the cells was judged by their growth after a further 24-h incubation. in ou ...19989583277
the role of gardnerella vaginalis in nonspecific vaginitis in intra uterine contraceptive device users.two hundred forty patients who had intra uterine contraceptive device (iucd) and manifested of nonspecific vaginitis were investigated for the presence of g. vaginalis. pure growth of this organism was obtained in 14(5.8%) cases while 116(48.3%) cases showed this organism in association with other organisms e.g. esch. coli (11.7%), klebsiella (9.2%), candida (9.2%), strept. faecalis (7.3%), proteus species (5.8%) and staph. albus (5%).19989581079
translocated intestinal bacteria cause spontaneous bacterial peritonitis in cirrhotic rats: molecular epidemiologic evidence.intestinal bacterial translocation is common in cirrhotic rats with spontaneous bacterial peritonitis, and it is thought to play a major pathogenic role. there has so far been no evidence for clonality between bacteria isolated from intestine and ascites. this study aimed to use molecular epidemiology techniques to show that spontaneous bacterial peritonitis is due to translocated intestinal bacteria.19989580278
abscess formation within a parasagittal meningioma. case report.the authors present the case of a 78-year-old woman who developed right lower-extremity paralysis after a focal seizure. neuroradiological studies revealed a small parasagittal meningioma, which at the time of resection was found to contain a bacterial intratumoral abscess secondary to proteus mirabilis. this is only the second reported case of intratumoral abscess formation in a meningioma and the first such occurrence to be reported in an otherwise healthy, immunocompetent individual.19989576260
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1995). ii. background of patients].clinical background was investigated on patients with urinary tract infections (utis) from whom 785 bacterial strains were isolated in 11 hospitals during the period from june, 1995 through may, 1996. 1. distributions of age and sex of patients and type of infections. among the patients examined, those with ages 50 years or older were the most frequent (males: 80.5%, females: 69.7%), and, among females, those with ages in the 20's were 12.6%. with regard to types of infections, more than a half ...19979575354
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1995). i. susceptibility distribution].the frequencies of isolation and susceptibilities to antimicrobial agents were investigated on 704 bacterial strains isolated from patients with urinary tract infections (utis) in 11 hospitals during the period of june 1995 to may 1996. of the above bacterial isolates, gram-positive bacteria accounted for 29.8% and a majority of them were enterococcus faecalis. gram-negative bacteria accounted for 70.2% and most of them were escherichia coli. susceptibilities of several isolated bacteria to anti ...19979575353
[hinman syndrome: an entity to keep in mind]. 19979575130
regulatory conservation and divergence of sigma32 homologs from gram-negative bacteria: serratia marcescens, proteus mirabilis, pseudomonas aeruginosa, and agrobacterium tumefaciens.the heat shock response in escherichia coli is mediated primarily by the rpoh gene, encoding sigma32, which is specifically required for transcription of heat shock genes. a number of sigma32 homologs have recently been cloned from gram-negative bacteria that belong to the gamma or alpha subdivisions of the proteobacteria. we report here some of the regulatory features of several such homologs (rpoh) expressed in e. coli as well as in respective cognate bacteria. when expressed in an e. coli del ...19989573192
adhesion-dependent f-actin pattern in amoeba proteus as a common feature of amoebae and the metazoan motile cellsadhesion and movement of amoeba proteus are both dependent on the appropriate arrangement of the f-actin cytoskeleton and on the presence of the cell nucleus. in this study the f-actin organization was examined by routine fitc-phalloidin staining and confocal laser microscopy in intact amoebae and in their nucleated and anucleated fragments, at different levels of cell adherence to the substratum. in the adhering and migrating intact cells and nucleated cell fragments dot-like aggregates of f-ac ...19979570891
surface characteristics of gram-negative and gram-positive bacteria in an atomic force microscope image.bacterial images can be obtained rather easily with an atomic-force microscope (afm) in the magnification range of 5,000 to 30,000 times without any pretreatment of the specimens for such observations as chemical fixation, dehydration or staining. the bacterial shapes or the presence of flagella can be clearly recognized in these magnification ranges. in addition, we were also able to distinguish between gram-negative and gram-positive bacteria based on the specific wavy surface appearance of th ...19989570280
a mild form of proteus syndrome.proteus syndrome is a rare congenital hamartomatous syndrome. we report on the clinical and radiological appearances of a boy in order to illustrate the typical signs which include subcutaneous masses, in mild forms partial gigantism of hands and feet, hemihypertrophy, and bony abnormalities. we discuss how to make the definitive diagnosis on the basis of using a known rating scale, important aspects of differential diagnosis and clinical features, and diagnostic management.19989569327
does circumcision alter the periurethral bacterial flora?a prospective study of 25 boys who underwent circumcision for medical reason was performed. specimens of periurethral bacterial flora were taken before operation as well as 3 weeks after surgery, so that each boy acted as his own control. before circumcision, 13 (52%) harboured uropathogenic organisms (escherichia coli and other coliforms, enterococcus spp, proteus spp, pseudomonas spp, and klebsiella spp); after circumcision, none of the boys had uropathogens, the only organisms cultured from t ...19989563029
in vitro interaction of the ihf-like proteins acinetobacter junii and proteus vulgaris with ihf sites.the ability of the ihf-like proteins of acinetobacter junii and proteus vulgaris to interact with the h' attp and pr' ihf sites of lambda dna was investigated. ihf from a. junii and p. vulgaris was found to bind the examined ihf sites in a way similar to ihf from escherichia coli as shown by gel mobility shift dna binding assays and footprinting analysis. the three ihf proteins bound to the pr' ihf site that overlaps the-35 region of that promoter and in vitro repression of transcription by each ...19989561747
results of quantitative cultures of urine by free catch and catheterization from healthy adult horses.quantitative urine cultures were performed on 11 male and 11 female healthy adult horses. urine was collected by free catch and catheterization using standard methods. results showed that all samples collected by free catch contained less than 20,000 colony-forming units (cfu)/ml. all samples collected by catheterization contained 500 cfu/ml or less. a significant difference was found between collection methods (p < .005), with catheterization having less contamination. in samples collected by f ...19989560762
successful treatment with polymyxin b immobilized fiber of septic shock complicated by renal stone.we describe a patient with septic shock that was spontaneously complicated by calyceal diverticular calculus. the patient recovered from shock within a few days with treatment by extracorporeal elimination of endotoxin using a blood purification cartridge consisting of polymyxin b immobilized fiber. standard open nephrolithotomy was performed, and the patient was discharged from the hospital 2 months after admission.19989559848
antibodies to klebsiella pneumoniae in dutch patients with ankylosing spondylitis and acute anterior uveitis and to proteus mirabilis in rheumatoid arthritis.to determine whether the association between increased humoral reactivity against klebsiella and hla-b27 associated diseases could be confirmed in dutch patients with ankylosing spondylitis (as) and acute anterior uveitis (aau).19989558179
[in vitro antibacterial activities of cefteram and other beta-lactam agents against recent clinical isolates].in vitro antibacterial activity of the third-generation oral cephem cefteram (cftm)--ten years after its first use in the clinical setting--against recent clinical isolates was evaluated and compared with those of other oral cephems. a total of 851 clinical isolates belonging to 13 species used in this study were collected from five medical institutions across japan during 1996. cftm showed excellent antibacterial activity against methicillin-susceptible s. aureus and s. pyogenes, equivalent to ...19989557273
the periplasmic, group iii catalase of vibrio fischeri is required for normal symbiotic competence and is induced both by oxidative stress and by approach to stationary phase.the catalase gene, kata, of the sepiolid squid symbiont vibrio fischeri has been cloned and sequenced. the predicted amino acid sequence of kata has a high degree of similarity to the recently defined group iii catalases, including those found in haemophilus influenzae, bacteroides fragilis, and proteus mirabilis. upstream of the predicted start codon of kata is a sequence that closely matches the consensus sequence for promoters regulated in escherichia coli by the alternative sigma factor enco ...19989555890
[establishing the types of dienes compatibility in clinical proteus mirabilis strains].the aim of the study was to use the dienes phenomenon for the differentiation of proteus mirabilis strains isolated from urine by the method of making possible their typing. the subject of the study were 351 strains of proteus mirabilis obtained from the laboratory of the institute of pediatrics, medical academy in lódź. for establishing of the compatibility type 18 indicator strains were taken, each of them had action antagonistic towards all other ones. the results were read according to the p ...19979554149
[virulence factors and host responses in urinary tract infections].pathogenicity of urinary tract infection (uti) has been reviewed mainly focussing on the bacterial adherence in uropathogens and the inflammatory responses of urinary mucosa. bacterial fimbriae or pili, which are encoded by chromosomal gene clusters played a major role in the adherence mechanisms of uropathogenic bacteria, including escherichia coli. furthermore, inflammatory responses in urinary mucosa including cytokine release was also investigated. the latest information concerning pathogeni ...19989551528
crystal structure of tryptophanase.the x-ray structure of tryptophanase (tnase) reveals the interactions responsible for binding of the pyridoxal 5'-phosphate (plp) and atomic details of the k+ binding site essential for catalysis. the structure of holo tnase from proteus vulgaris (space group p2(1)2(1)2(1) with a = 115.0 a, b = 118.2 a, c = 153.7 a) has been determined at 2.1 a resolution by molecular replacement using tyrosine phenol-lyase (tpl) coordinates. the final model of tnase, refined to an r-factor of 18.7%, (rfree = 22 ...19989551100
proteus penneri urosepsis in a patient with diabetes mellitus.proteus penneri has been isolated from many different clinical sources, including surgical wound infections, urine, and blood. we describe the first reported case of p. penneri nosocomial urosepsis in a patient with diabetes. p. penneri was subsequently isolated from bronchoalveolar lavage fluid and a pulmonary artery catheter tip.19989548071
antimicrobial activity of organic thiosulfates (bunte salts).a number of organic thiosulfates (bunte salts) were prepared from appropriate primary bromides or iodides. in the case of substrates with long aliphatic chains, an addition of benzyltrimethylammonium chloride as phase transfer catalyst was very successful. the bunte salts obtained were tested for antibacterial and fungicidal activity by means of the agar disc-diffusion method and by assignation of the minimum inhibitory concentrations (mic). it was found that the microorganisms proteus vulgaris, ...19989547520
evaluation of use of a new chromogenic agar in detection of urinary tract pathogens.chromagar orientation, a new chromogenic medium, was evaluated for the detection and differentiation of gram-positive and gram-negative pathogenic microorganisms in 900 urine samples from hospitalized patients. performance characteristics of the medium were evaluated in comparison to those of 5% sheep blood and macconkey agars by direct inoculation of the urine samples on the three media. four gram-negative and two gram-positive strains as well as one yeast control strain from the american type ...19989542923
antibiotic sensitivity and proticine typing of proteus mirabilis strains associated with rheumatoid arthritis.urinary isolates of proteus mirabilis, obtained from 49 ra patients and 44 healthy controls, were tested for susceptibility to antibiotics by the disc diffusion method. in addition, p. mirabilis isolates were also tested for proticine production and sensitivity (p/s) typing by the inhibition of growth of each test isolate against 13 reference strains of p. mirabilis. the p. mirabilis isolates from both ra patients and healthy controls were highly susceptible to norfloxacin, ciprofloxacin and tri ...19989542782
[risk factors and pathogenic microorganisms in patients with insufficient esophagojejunostomy after gastrectomy].it was the aim of the study to find by retrospective analysis of data from totally gastrectomized patients risk factors for the development of esophago-jejunal anastomotic leakage, that may be avoidable or influenced therapeutically.19989542030
cellulitis caused by citrobacter diversus in a patient with multiple myeloma.the most common cause of cellulitis is streptococci. coagulase-negative staphylococci and gram-negative bacteria, such as serratia spp., proteus spp., and other enterobacteriaceae may produce cellulitis in the immunocompromised patient. we report a case of citrobacter diversus-induced cellulitis, resembling streptococcal infection, in a patient with multiple myeloma.19989538959
molecular mechanisms of polymyxin b-membrane interactions: direct correlation between surface charge density and self-promoted transport.we have studied the interaction of the polycationic peptide antibiotic polymyxin b (pmb) with asymmetric planar bilayer membranes via electrical measurements. the bilayers were of different compositions, including those of the lipid matrices of the outer membranes of various species of gram-negative bacteria. one leaflet, representing the bacterial inner leaflet, consisted of a phospholipid mixture (pl; phosphatidylethanolamine, -glycerol, and diphosphatidylglycerol in a molar ratio of 81:17:2). ...19989538506
prevalence and sensitivity to antibiotics of enterobacteriaceae isolated from urinary cultures in some microbiology laboratories of a city in west greece.the prevalence of enterobacteriaceae from midstream urine samples from patients with community acquired urinary tract infections (uti) of a town in sw greece during one year period and their susceptibility to antibiotics were studied. the most frequently recovered pathogens were e. coli (77%), proteus mirabilis (10%), klebsiella spp (8.7%), enterobacter spp (2.5%) and citrobacter freundii (1.8%). e. coli were found more resistant to carbenicillin, the combination of amoxicillin/clavulanic acid a ...19979538469
paediatric xanthogranulomatous pyelonephritis.xanthogranulomatous pyelonephritis (xgp) is a well recognised but rare type of chronic pyelonephritis classically occurring in middle-aged women. it is increasingly being recognised in children in whom it can be mistaken for wilms' tumour. awareness of the possibility of this condition occurring in children may allow early recognition and possible treatment. two cases of xgp in children are described and the literature reviewed.19989536568
enterobacterial antibodies in chinese patients with rheumatoid arthritis and ankylosing spondylitis.to study the role of microbial infection in rheumatic diseases.19989536392
two nosocomial strains of stenotrophomonas maltophilia transferring antibiotic resistance to proteus mirabilis p-38 recipient strain.in this report we describe a specific transfer of carbenicillin and cephaloridine resistance determinants from two different strains of stenotrophomonas maltophilia: no. 215 and 221 isolated from two critically ill patients treated in different intensive care units of a large university hospital in ostrava, czech republic. these strains were resistant to flouroquinolones and the following beta-lactam drugs: carbenicillin, cephaloridine, cefotaxime, ceftazidime, cefepime, imipenem, meropenem and ...19989531071
pre-labour rupture of membrane: the histological study of membrane and bacteriological profile.one hundred two (102) cases of pre-labour rupture of membrane (prom) were studied and special attention was given to the histological study of the amniotic membrane as well as to the bacteriological study of high vaginal flora, cervical flora and flora of amniotic fluid, in search of probable causes or factors leading to prom. the incidence was found to be 3.16% in the age group of 20-25 years without any relation to parity; and the duration of gestation was 38 to 40 weeks in most of the cases. ...19979529583
mrpb functions as the terminator for assembly of proteus mirabilis mannose-resistant proteus-like fimbriae.insertional mutagenesis studies of mrpb, a putative pilin-encoding open reading frame of the mrp gene cluster, which encodes mannose-resistant proteus-like (mr/p) fimbriae of proteus mirabilis, indicate that mrpb functions as the terminator for fimbrial assembly.19989529110
microbiology of necrotizing fasciitis associated with omphalitis in the newborn infant.the purpose of this study was to report the aerobic and anaerobic microbiology of periumbilical necrotizing fasciitis in newborn infants.19989527941
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