Publications
Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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efficacy of lytic bacteriophage preparation in reducing salmonella in vitro, on turkey breast cutlets, and on ground turkey. | the efficacy of the recently approved salmonella lytic bacteriophage preparation (salmofresh) in reducing salmonella enterica serotype heidelberg on turkey breast cutlets and ground turkey was evaluated. in a broth model assay, the phage preparation completely inhibited the growth of four s. enterica serotypes (salmonella enteritidis, salmonella heidelberg, salmonella kentucky, and salmonella typhimurium) at 37°c at a multiplicity of infection of 10,000 pfu/cfu. at 4°c in 0.1% peptone water (pw) ... | 2015 | 26197288 |
effects of 5-hydroxytryptophan and m-hydroxybenzylhydrazine associated to lactobacillus spp. on the humoral response of broilers challenged with salmonella enteritidis. | this study investigates the effects of different doses of serotonin, its precursor 5-hydroxytry-ptophan (5htp), and m-hydroxybenzylhydrazine inhibitor (nsd1015), administered via intraperitoneal for 5 consecutive days, on behavior and average body weight of broilers. we also measured the humoral immune response and quantification of salmonella enteritidis in broilers chickens that received the drugs evaluated and a lactobacillus pool. the study was divided into 3 experiments: experiment 1--admin ... | 2015 | 26195810 |
recovery of salmonella enterica serovar enteritidis from hens initially infected with serovar kentucky. | salmonella enterica serovars enteritidis and kentucky differ greatly in epidemiology. we wanted to know if the non-pathogenic serotype kentucky impacted the recovery of the pathogen enteritidis from chickens. to explore this issue, 4 groups of hens were treated as follows: (i) hens were inoculated orally with kentucky and injected intramuscularly 2 weeks later with enteritidis, (ii) hens were contact infected with kentucky and then with enteritidis, (iii) hens were injected with enteritidis only ... | 2015 | 26190605 |
modeling disinfection of plastic poultry transport cages inoculated with salmonella enteritids by slightly acidic electrolyzed water using response surface methodology. | in order to reduce the risk of enteric pathogens transmission in animal farms, the disinfection effectiveness of slightly acidic electrolyzed water (saew, ph 5.85 to 6.53) for inactivating salmonella enteritidis on the surface of plastic poultry transport cages was evaluated. the coupled effects of the tap water cleaning time (5 to 15 s), saew treatment time (20 to 40 s), and available chlorine concentrations (accs) of 30 to 70 mg/l on the reductions of s. enteritidis on chick cages were investi ... | 2015 | 26188036 |
curcuma and scutellaria plant extracts protect chickens against inflammation and salmonella enteritidis infection. | after a ban on the use of antibiotics as growth promoters in farm animals in the european union in 2006, an interest in alternative products with antibacterial or anti-inflammatory properties has increased. in this study, we therefore tested the effects of extracts from curcuma longa and scutellaria baicalensis used as feed additives against cecal inflammation induced by heat stress or salmonella enteritidis (s. enteritidis) infection in chickens. curcuma extract alone was not enough to decrease ... | 2015 | 26188032 |
temperature-sensitive salmonella enterica serovar enteritidis pt13a expressing essential proteins of psychrophilic bacteria. | synthetic genes based on deduced amino acid sequences of the nad-dependent dna ligase (liga) and ctp synthetase (pyrg) of psychrophilic bacteria were substituted for their native homologues in the genome of salmonella enterica serovar enteritidis phage type 13a (pt13a). the resulting strains were rendered temperature sensitive (ts) and did not revert to temperature resistance at a detectable level. at permissive temperatures, ts strains grew like the parental strain in broth medium and in macrop ... | 2015 | 26187965 |
upregulation of nramp1 mrna confirms its role in enhanced host immunity in post-artificial infections of salmonella enteritidis in chicks. | 1. salmonella enteritidis (se) is reported as the most common food-borne pathogen transmitted through poultry products. the natural resistance-associated macrophage protein 1 (nramp1) is a candidate gene associated with se-mediated immune response and is related to the phagocytosis of se. in this study, the classical single-nucleotide polymorphism (snp) g2357a in exon 8 of the nramp1 gene was detected. the expression of nramp1 mrna was first investigated in heterophil granulocytes and spleen in ... | 2015 | 26181686 |
chemical modification of a dehydratase enzyme involved in bacterial virulence by an ammonium derivative: evidence of its active site covalent adduct. | the first example of an ammonium derivative that causes a specific modification of the active site of type i dehydroquinase (dhq1), a dehydratase enzyme that is a promising target for antivirulence drug discovery, is described. the resolution at 1.35 å of the crystal structure of dhq1 from salmonella typhi chemically modified by this ammonium derivative revealed that the ligand is covalently attached to the essential lys170 through the formation of an amine. the detection by mass spectroscopy of ... | 2015 | 26148116 |
reducing colonization and eggborne transmission of salmonella enteritidis in layer chickens by in-feed supplementation of caprylic acid. | salmonella enteritidis (se) is a major foodborne pathogen responsible for causing gastrointestinal infections in humans, predominantly due to the consumption of contaminated eggs. in layer hens, se colonizes the intestine and migrates to various organs, including the oviduct, thereby leading to egg yolk and shell contamination. this study investigated the efficacy of caprylic acid (ca), a medium-chain fatty acid, in reducing se colonization and egg contamination in layers. caprylic acid was supp ... | 2015 | 26135893 |
high incubation temperature and threonine dietary level improve ileum response against post-hatch salmonella enteritidis inoculation in broiler chicks. | this study assessed the effect of both embryonic thermal manipulation and dietary threonine level on the response of broilers inoculated with salmonella enteritidis, considering bacterial counts in the cecal contents, intestinal morphology, mucin and heat shock protein 70 gene expression, body weight and weight gain. thermal manipulation was used from 11 days of incubation until hatch, defining three treatments: standard (37.7°c), continuous high temperature (38.7°c) and continuous low temperatu ... | 2015 | 26131553 |
comparison of a live attenuated salmonella enteritidis vaccine candidate secreting escherichia coli heat-labile enterotoxin b subunit with a commercial vaccine for efficacy of protection against internal egg contamination by salmonella in hens. | this study compared a new live attenuated salmonella enteritidis vaccine candidate secreting escherichia coli heat-labile enterotoxin b subunit (se-ltb) with a commercial salmonella enteritidis (se) vaccine for efficacy of protection against se infection in laying hens. chickens were divided into 3 groups of 20 each. group a chickens were inoculated orally with phosphate-buffered saline and served as controls, group b chickens were inoculated orally with the vaccine candidate, and group c chicke ... | 2015 | 26130857 |
first description of plasmid-mediated quinolone resistance determinants and β-lactamase encoding genes in non-typhoidal salmonella isolated from humans, one companion animal and food in romania. | gastroenteritis attributable to salmonella enterica and the continuous increase in antimicrobial resistance of this gut pathogen, which compromises the use of previously effective treatments, is of great concern for public health. this study was conducted in order to investigate the presence of plasmid-mediated quinolone resistance (pmqr) determinants and β-lactamase-encoding genes, in s. enterica, isolated from humans, one companion animal and food. moreover, the study aimed to identify potenti ... | 2015 | 26120367 |
use of flow cytometry for quantitative analysis of metabolism of viable but non-culturable (vbnc) salmonella. | a simple and rapid assay method for analysis of the metabolic activity of viable but non-culturable (vbnc) salmonella was established. an environmental isolate of salmonella enteritidis (se), grown to the logarithmic phase, rapidly lost its culturability during incubation with 1-10 mm h2o2 in luria-bertani (lb) medium. to assess the viability of the bacteria, we measured 3 different metabolic activities: respiratory activity by 5-cyano-2,3-ditolyl-tetrazolium chloride (ctc) reduction, glucose up ... | 2015 | 26119425 |
mucosal immunization of balb/c mice with dna vaccines encoding the sen1002 and sen1395 open reading frames of salmonella enterica serovar enteritidis induces protective immunity. | salmonella enteritidis is the main cause of foodborne salmonellosis worldwide. the limited effectiveness of current interventions against this pathogen has been the main incentive to develop new methods for the efficient control of this infection. to investigate the use of dna vaccines against s. enteritidis in humans, immune responses stimulated by two plasmids containing the genes designated sen1002, located in the pathogenicity island spi-19 and encoding a hcp protein involved in transport me ... | 2016 | 26113459 |
characterization of a foodborne outbreak caused by salmonella enteritidis in aracaju, state of sergipe, brazil. | in december 2001, an outbreak of foodborne gastroenteritis infected 114 of 161 people who ate at a restaurant in aracaju, state of sergipe, brazil. | 2015 | 26108014 |
immunomodulatory activity and control of salmonella enteritidis colonization in the intestinal tract of chickens by lactobacillus based probiotic. | lactobacillus-based probiotics (lbp) are used as competitive exclusion to control pathogenic enterobacterial infections and improve the weight gain in broiler chickens. this study assessed the inhibition of salmonella enteritidis (se) infection in one-week-old broiler chicks, using an experimental lbp containing four lactobacillus strains isolated from chickens (l. acidophilus, l. fermentum, l. reuteri, l. salivarius). the immunomodulatory effects of this treatment were evaluated, through the an ... | 2015 | 26099807 |
iga gene expression and quantification of cecal iga+, igm+, and cd4+ cells in chickens treated with efal41 and infected with salmonella enteritidis. | iga gene expression and quantification of mucous iga+, igm+ and cd4+ lymphocytes in the cecum of chicks was studied by qrt-pcr, immunohistochemistry and flow cytometry. a total of 220 1-day-old salmonella-free chicks of cobb 500 were divided into four groups (n=55). group 1 served as control (c), group 2 was pretreated with probiotic bacterial strain enterococus faecium al41 (efal41), group 3 was infected with salmonella enteritidis pt4 (se), and group 4 was pretreated with e. faecium al41 and s ... | 2015 | 26093882 |
antimicrobial activity of thyme oil co-nanoemulsified with sodium caseinate and lecithin. | emulsions of essential oils are investigated as potential intervention strategies to improve food safety and are preferably prepared from generally-recognized-as-safe emulsifiers. stable thyme oil nanoemulsions can be prepared using combinations of sodium caseinate (nacas) and soy lecithin. the objective of the present research was to study the antimicrobial activity of these nanoemulsions and understand the impacts of emulsifier concentrations. 10 g/l thyme oil was emulsified using combinations ... | 2015 | 26082324 |
an enterobacterial common antigen mutant of salmonella enterica serovar typhimurium as a vaccine candidate. | due to increasing rates of invasive salmonella enterica serovar typhimurium infection, there is a need for an effective vaccine to prevent this disease. previous studies showed that a mutation in the first gene of the enterobacterial common antigen biosynthetic pathway, weca, resulted in attenuation of s. typhimurium in a murine model of salmonellosis. furthermore, immunization with a weca(-) strain protected against lethal challenge with the parental wild type s. typhimurium strain. herein, we ... | 2015 | 26070977 |
herd- and individual-level prevalences of and risk factors for salmonella spp. fecal shedding in dairy farms in al-dhulail valley, jordan. | salmonellosis is an important disease frequently associated with diarrhea in calves. from january to september 2009, a cross-sectional study involving 91 dairy farms was conducted to determine the prevalence of salmonella spp. infection in cattle in al-dhulail valley, jordan. a total of 910 calve and cow fecal samples were collected. information on farm management practices was obtained through personal interviews using a standardized questionnaire and was tested as risk factors for salmonella s ... | 2015 | 26065698 |
molecular epidemiology of nontyphoidal salmonella in poultry and poultry products in india: implications for human health. | human infections with non-typhoidal salmonella (nts) serovars are increasingly becoming a threat to human health globally. while all motile salmonellae have zoonotic potential, salmonella enteritidis and salmonella typhimurium are most commonly associated with human disease, for which poultry are a major source. despite the increasing number of human nts infections, the epidemiology of nts in poultry in india has not been fully understood. hence, as a first step, we carried out epidemiological a ... | 2015 | 26063942 |
close genetic relationship between salmonella enterica serovar enteritidis isolated from patients with diarrhoea and poultry in the republic of korea. | 2015 | 26055417 | |
effect of salmonella infection on cecal tonsil regulatory t cell properties in chickens. | two studies were conducted to study regulatory t cell [treg (cd4⁺cd25⁺)] properties during the establishment of a persistent intestinal infection in broiler chickens. four-day-old broiler chicks were orally gavaged with 5 × 10⁶ cfu/ml salmonella enteritidis (s. enteritidis) or sterile pbs (control). samples were collected at 4, 7, 10, and 14 d postinfection. there was a significant (p < 0.05) increase in the number of cd4⁺cd25⁺ cells by d 4 postinfection that increased steadily throughout the co ... | 2015 | 26049799 |
gene expression profiles of chicken embryo fibroblasts in response to salmonella enteritidis infection. | the response of chicken to non-typhoidal salmonella infection is becoming well characterised but the role of particular cell types in this response is still far from being understood. therefore, in this study we characterised the response of chicken embryo fibroblasts (cefs) to infection with two different s. enteritidis strains by microarray analysis. the expression of chicken genes identified as significantly up- or down-regulated (≥3-fold) by microarray analysis was verified by real-time pcr ... | 2015 | 26046914 |
[use of bacteriphages against salmonella enteritidis: a prevention tool]. | salmonellosis is a highly prevalent disease still searching for preventive tools to avoid contamination level priority public health. | 2015 | 26040389 |
detection of poultry meat specific bacteria using ftir spectroscopy and chemometrics. | ftir spectra of poultry meat specific bacteria viz. salmonella enteritidis, pseudomonas ludensis, listeria monocytogenes and escherichia coli were collected and investigated for identification of spectral windows capable of bacterial classification and quantification. two separate datasets obtained at different times were used in the study to check reproducibility of results. multivariate data analysis techniques viz. principal component analysis (pca), partial least-squares discriminant analysi ... | 2015 | 26028771 |
multinational outbreak of salmonella enteritidis infection during an international youth ice hockey competition in riga, latvia, preliminary report, march and april 2015. | a multinational outbreak of salmonellosis linked to the riga cup 2015 junior ice-hockey competition was detected by the finnish health authorities in mid-april and immediately notified at the european union level. this prompted an international outbreak investigation supported by the european centre for disease prevention and control. as of 8 may 2015, seven countries have reported 214 confirmed and suspected cases, among which 122 from finland. the search for the source of the outbreak is ongoi ... | 2015 | 26027481 |
rapid draft sequencing and real-time nanopore sequencing in a hospital outbreak of salmonella. | foodborne outbreaks of salmonella remain a pressing public health concern. we recently detected a large outbreak of salmonella enterica serovar enteritidis phage type 14b affecting more than 30 patients in our hospital. this outbreak was linked to community, national and european-wide cases. hospital patients with salmonella are at high risk, and require a rapid response. we initially investigated this outbreak by whole-genome sequencing using a novel rapid protocol on the illumina miseq; we the ... | 2015 | 26025440 |
efficacy of fumigation with trans-cinnamaldehyde and eugenol in reducing salmonella enterica serovar enteritidis on embryonated egg shells. | this study investigated the efficacy of two gras (generally regarded as safe)-status, plant-derived antimicrobials (pdas), namely trans-cinnamaldehyde (tc) and eugenol (eug) applied as a fumigation treatment in reducing se on embryonated egg shells. egg shells of day-old embryonated eggs were spot inoculated with a 4-strain mixture of se (∼6.5 log cfu/egg) and subjected to fumigation with the aforementioned pdas (0 or 1% concentration) for 20 minutes in a hatching incubator. se on the shell and ... | 2015 | 26009758 |
detection of cell surface hydrophobicity, biofilm and fimbirae genes in salmonella isolated from tunisian clinical and poultry meat. | the aim of this study was to evaluate the ability of 15 serotypes of salmonella to form biofilm on polystyrene, polyvinyl chloride (pvc) and glass surfaces. . | 2014 | 26005652 |
incorporation of nisin z and lauric arginate into pullulan films to inhibit foodborne pathogens associated with fresh and ready-to-eat muscle foods. | a combination of food grade compounds with edible films, used to inhibit foodborne pathogens associated with fresh or further processed muscle foods, is receiving considerable attention. in this study, pullulan films containing lauric arginate (lae) and nisin z (produced by lactococcus lactis subsp. lactis i8-7-3 and isolated from catfish gut), alone or in combination, were investigated for controlling foodborne pathogens on fresh and further processed muscle foods after long-term refrigerated s ... | 2015 | 26001063 |
a mutation in the nlrc5 promoter limits nf-κb signaling after salmonella enteritidis infection in the spleen of young chickens. | to date, the functions of the nlrc5 in chickens remain undefined. in the current study, chicken nlrc5 was cloned and an a1017g mutation was detected in its promoter region. the relative expression levels of the nlrc5 and key nf-κb pathway genes, ikkα, ikkβ, nf-κb, il-6, il-1β and ifn-γ, in the spleens of wild and mutant type birds, aa and gg, were determined using fq-pcr at 7 day post-infection (dpi) with salmonella enteritidis. additionally, the bacterial burden in the caecum and various immune ... | 2015 | 25979675 |
antimicrobial activity and chemical composition of the essential oils of portuguese foeniculum vulgare fruits. | the aim of this study was to investigate the chemical composition and antimicrobial activity of essential oils obtained by hydrodistillation from fruits of six fennel accessions collected from wild populations occurring in the centre and south of portugal. composition of essential oils was established by gas chromatography-flame ionization detector (gc-fid) and gas chromatography-mass spectrometry (gc-ms) analysis. the obtained yields of the essential oils were found to vary greatly in the range ... | 2015 | 25973507 |
identification and functional characterization of toll-like receptor 2-1 in geese. | toll-like receptor 2 (tlr2), an important pattern recognition receptor, activates proinflammatory pathways in response to various pathogens. it has been reported in humans and chicken, but not in geese, an important waterfowl species in china. since some vaccines stimulate robust immune responsesl in chicken but not in geeeses we speculated that their immune systems are different. | 2015 | 25967535 |
a new repertoire of informations about the quorum sensing system in salmonella enterica serovar enteritidis pt4. | salmonella spp are among the main causative agents of foodborne diseases. some phenotypes associated with increased drug resistance and virulence are regulated by quorum sensing (qs). in the present study, the autoinducer (ai)-1- and -2-mediated qs mechanisms were characterized in salmonella enterica serovar enteritidis pt4 for the first time. salmonella enteritidis did not produce ai-1. phylogenetic analysis of nucleotides encoding the sdia protein, the response regulator of ai-1-mediated qs, a ... | 2015 | 25966179 |
[study on simultaneous contamination of salmonella and campylobacter in retail chicken carcasses in beijing]. | to elucidate the simultaneous contamination of salmonella and campylobacter in retail chicken carcasses in beijing and to carry out the serological typing of all salmonella isolates as well as the identification of campylobacter at the species level. | 2015 | 25958640 |
a multi-country salmonella enteritidis phage type 14b outbreak associated with eggs from a german producer: 'near real-time' application of whole genome sequencing and food chain investigations, united kingdom, may to september 2014. | we report an outbreak of salmonella enteritidis phage type 14b (pt14b) in the united kingdom (uk) between may and september 2014 where public health england launched an investigation to identify the source of infection and implement control measures. during the same period, outbreaks caused by a salmonella enteritidis strain with a specific multilocus variable-number tandem repeat analysis (mlva) profile occurred in other european union member states. isolates from a number of persons affected b ... | 2015 | 25953273 |
composition, antimicrobial, antioxidant, and antiproliferative activity of origanum dictamnus (dittany) essential oil. | nowadays, there has been an increased interest in essential oils from various plant origins as potential antimicrobial, antioxidant, and antiproliferative agents. this trend can be mainly attributed to the rising number and severity of food poisoning outbreaks worldwide along with the recent negative consumer perception against artificial food additives and the demand for novel functional foods with possible health benefits. origanum dictamnus (dittany) is an aromatic, tender perennial plant tha ... | 2015 | 25952773 |
interaction of tgf-β4 and il-17 with iga secretion in the intestine of chickens fed with e. faecium al41 and challenged with s. enteritidis. | the relative mrna expression of iga, tgf-β4, il-17, and concentration of secretory iga (siga) in small intestine of chickens pretreated with enterococcus faecium al41 and challenged with salmonella enteritidis pt4 were studied. salmonella-free day-old chicks (40) cobb 500 breed, were divided into four groups of 10 chicks each (n = 10): control (c), treated with e. faecium al41 strain (efal41), challenged with salmonella enteritidis pt4 (se), and combined (efal41+se). expression of iga and siga c ... | 2015 | 25935756 |
membrane cholesterol plays an important role in enteropathogen adhesion and the activation of innate immunity via flagellin-tlr5 signaling. | lipid rafts are cholesterol- and sphingolipid-rich ordered microdomains distributed in the plasma membrane that participates in mammalian signal transduction pathways. to determine the role of lipid rafts in mediating interactions between enteropathogens and intestinal epithelial cells, membrane cholesterol was depleted from caco-2 and ipec-j2 cells using methyl-β-cyclodextrin. cholesterol depletion significantly reduced escherichia coli and salmonella enteritidis adhesion and invasion into inte ... | 2015 | 25935453 |
the prevalence and plasmid profile of non-typhoidal salmonellosis in children in lagos metropolis, south-western nigeria. | non-typhoidal salmonella is the causative agent of gastroenteritis, a food-borne and zoonotic infection which is a major cause of high morbidity and death among children under 5 years of age especially from resource poor settings like the developing countries. | 2014 | 25932072 |
antibacterial activity of lactobacillus spp. isolated from the feces of healthy infants against enteropathogenic bacteria. | lactobacilli are normal microflora of the gastrointestinal (gi) tract and are a heterogeneous group of lactic acid bacteria (lab). lactobacillus strains with probiotic activity may have health benefits for human. this study investigates the probiotic potential of lactobacillus strains obtained from the feces of healthy infants and also explores antibacterial activity of lactobacillus strains with probiotic potential against enteropathogenic bacteria. fecal samples were collected from 95 healthy ... | 2015 | 25930687 |
type 1 fimbriae are important factors limiting the dissemination and colonization of mice by salmonella enteritidis and contribute to the induction of intestinal inflammation during salmonella invasion. | we have recently shown that salmonella gallinarum type 1 fimbriae with endogenous mannose-resistant (mr) variant of the fimh protein increase systemic dissemination of s. gallinarum and colonization of internal organs in comparison to the s. gallinarum fimh knockout strain or the mutant expressing mannose-sensitive (ms) fimh variant from s. enteritidis. elaborating from these studies, we proposed that ms variants of fimh are advantageous in gastrointestinal infections, in contrast to mr fimh var ... | 2015 | 25914682 |
combining lactic acid spray with near-infrared radiation heating to inactivate salmonella enterica serovar enteritidis on almond and pine nut kernels. | the aim of this study was to investigate the efficacy of near-infrared radiation (nir) heating combined with lactic acid (la) sprays for inactivating salmonella enterica serovar enteritidis on almond and pine nut kernels and to elucidate the mechanisms of the lethal effect of the nir-la combined treatment. also, the effect of the combination treatment on product quality was determined. separately prepared s. enteritidis phage type (pt) 30 and non-pt 30 s. enteritidis cocktails were inoculated on ... | 2015 | 25911473 |
persistence of fecal shedding of salmonella enteritidis by experimentally infected laying hens housed in conventional or enriched cages. | salmonella enteritidis can be deposited inside eggs laid by infected hens, so the prevalence of this pathogen in commercial egg-producing flocks is an important risk factor for human illness. opportunities for the introduction, transmission, and persistence of salmonellae in poultry are potentially influenced by flock housing and management systems. animal welfare concerns have spurred the development of alternatives to traditional cage-based housing. however, the consequences of poultry housing ... | 2015 | 25910903 |
live attenuated vaccines for invasive salmonella infections. | salmonella enterica serovar typhi produces significant morbidity and mortality worldwide despite the fact that there are licensed salmonella typhi vaccines available. this is primarily due to the fact that these vaccines are not used in the countries that most need them. there is growing recognition that an effective invasive salmonella vaccine formulation must also prevent infection due to other salmonella serovars. we anticipate that a multivalent vaccine that targets the following serovars wi ... | 2015 | 25902362 |
improved cell surface display of salmonella enterica serovar enteritidis antigens in escherichia coli. | salmonella enterica serovar enteritidis (se) is one of the most potent pathogenic salmonella serotypes causing food-borne diseases in humans. we have previously reported the use of the β-autotransporter aida-i to express the salmonella flagellar protein h:gm and the se serotype-specific fimbrial protein sefa at the surface of e. coli as live bacterial vaccine vehicles. while sefa was successfully displayed at the cell surface, virtually no full-length h:gm was exposed to the medium due to extens ... | 2015 | 25889453 |
[an etiological survey and traceability analysis on a foodborne illness epidemic outbreak caused by salmonella enteritidis]. | 2015 | 25876498 | |
biological activities and dna interactions of amanita ovoidea. | amanita ovoidea (bull.) link (amanitaceae) is a well-known species due to its pleasant aroma and flavor since ancient times in the worldwide. this species is also known in turkey and people consume it extensively. | 2015 | 25874387 |
purification and characterization of a novel plantaricin, kl-1y, from lactobacillus plantarum kl-1. | three bacteriocins from lactobacillus plantarum kl-1 were successfully purified using ammonium sulfate precipitation, cation-exchange chromatography and reverse-phase hplc. the bacteriocin peptides kl-1x, -1y and -1z had molecular masses of 3053.82, 3498.16 and 3533.16 da, respectively. all three peptides were stable at ph 2-12 and 25 °c and at high temperatures of 80 and 100 °c for 30 min and 121 °c for 15 min. however, they differed in their susceptibility to proteolytic enzymes and their inhi ... | 2015 | 25862353 |
synthesis, characterization, crystal structure and antimicrobial activity of copper(ii) complexes with the schiff base derived from 2-hydroxy-4-methoxybenzaldehyde. | a novel schiff base, ethyl 4-[(e)-(2-hydroxy-4-methoxyphenyl)methylene-amino]benzoate (hl), was prepared and structurally characterized on the basis of elemental analyses, (1)h nmr, (13)c nmr, uv-vis and ir spectral data. six new copper(ii) complexes, [cu(l)(no3)(h2o)2] (1), [cu(l)2] (2), [cu(l)(oac)] (3), [cu2 (l)2cl2(h2o)4] (4), [cu(l)(clo4)(h2o)] (5) and [cu2(l2s)(clo4)(h2o)]clo4·h2o (6) have been synthesized. the characterization of the newly formed compounds was done by ir, uv-vis, epr, fab ... | 2015 | 25849802 |
differential innate immune responses of bovine peripheral blood leukocytes to salmonella enterica serovars dublin, typhimurium, and enteritidis. | the majority of salmonella serovars cause no clinical disease in cattle, while some are associated with severe disease. the objective of the current study was to determine the innate immune responses of bovine peripheral blood leukocytes exposed to salmonella enterica serovar dublin (bovine-specific), salmonella typhimurium (murine adapted, but zoonotic), and salmonella enteritidis (poultry host-adapted) in 3-week-old calves. all salmonella exposures increased cell surface cd14 and cd18 regardle ... | 2015 | 25847354 |
a gaseous acetic acid treatment to disinfect fenugreek seeds and black pepper inoculated with pathogenic and spoilage bacteria. | contamination of spices by pathogenic and/or spoilage bacteria can be deleterious to consumer's health and cause deterioration of foods, and inactivation of such bacteria is necessary for the food industry. the present study examined the effect of gaseous acetic acid treatment in reducing escherichia coli o157:h7, salmonella enteritidis and bacillus subtilis populations inoculated on fenugreek seeds and black pepper. treatment with gaseous acetic acid at 0.3 mmol/l, 0.6 mmol/l and 4.7 mmol/l for ... | 2015 | 25846935 |
identification of in vivo-induced genes during infection of chickens with salmonella enterica serovar enteritidis. | chickens are an important source of food worldwide and are often infected with food-poisoning serovars of salmonella enterica, frequently salmonella enteritidis (se), without exhibiting clinical signs of disease. ivi (in vivo induced) genes identified using in vivo-induced antigen technology (iviat) are expressed only during bacterial infection and have the potential value of identifying epidemic strains and antigens which can form the basis for sub-unit vaccine development. we applied iviat to ... | 2015 | 25843894 |
effects of sub-lethal concentrations of thyme and oregano essential oils, carvacrol, thymol, citral and trans-2-hexenal on membrane fatty acid composition and volatile molecule profile of listeria monocytogenes, escherichia coli and salmonella enteritidis. | the aim of this work was to investigate the modifications of cell membrane fatty acid composition and volatile molecule profiles of listeria monocytogenes, salmonella enteritidis, escherichia coli, during growth in the presence of different sub-lethal concentrations of thyme and oregano essential oils as well as carvacrol, thymol, trans-2-hexenal and citral. the results evidenced that the tested molecules induced noticeable modifications of membrane fatty acid profiles and volatile compounds pro ... | 2015 | 25842326 |
phenotypic and genotypic characterization of salmonella enterica serovar enteritidis isolates associated with a mousse cake-related outbreak of gastroenteritis in ningbo, china. | salmonella enterica subsp. enterica serovar enteritidis (salmonella enteritidis) is a major pathogen responsible for causing the largest number of sporadic cases and outbreaks of human salmonellosis worldwide. in this study, an outbreak of salmonella enteritidis involving 112 cases in ningbo, china was investigated with a combination of genotypic subtyping methods and phenotypic analysis. the pulsed-field gel electrophoresis and multilocus variable-number tandem-repeat analysis profiles showed t ... | 2015 | 25826691 |
evaluation of a salmonella enteritidis vaccine and related elisa for respective induction and assessment of acquired immunity to the vaccine and/or echinacea purpurea in awassi ewes. | the aim of this study was to evaluate an experimental salmonella enteritidis (se) bacterin and an indirect elisa system to assess quantitatively the acquired immunity in awassi ewes to the vaccine and/or echinacea purpurea (ep) dried roots. four treatments of the ewes were included in the experimental design, with 6 ewes/treatment. the first treatment (t1) had the controls that were non-vaccinated and non-treated with ep. the t2 ewes were only treated with ep. the t3 and t4 ewes were vaccinated ... | 2015 | 25820070 |
physiology, pathogenicity and immunogenicity of live, attenuated salmonella enterica serovar enteritidis mutants in chicks. | to construct a novel live, attenuated salmonella vaccine, the lon, cpxr and cpdb genes were deleted from a wild-type salmonella enterica serovar enteritidis-6 (sm-6) strain using the phage λ red homologous recombination system, resulting in sm-△cpxr, sm-△c/lon and sm-△c/l/cpdb. the growth curves of strain sm-△c/lon grew more rapidly than the other strains and had od 600 values higher than the other strains starting at the 4 h time point. the growth curves of strain sm-△c/l/cpdb were relatively f ... | 2015 | 25819881 |
zno nanoparticles impose a panmetabolic toxic effect along with strong necrosis, inducing activation of the envelope stress response in salmonella enterica serovar enteritidis. | in this study, we tested the antimicrobial activity of three metal nanoparticles (nps), zno, mgo, and cao nps, against salmonella enterica serovar enteritidis in liquid medium and on solid surfaces. out of the three tested metal nps, zno nps exhibited the most significant antimicrobial effect both in liquid medium and when embedded on solid surfaces. therefore, we focused on revealing the mechanisms of surface-associated zno biocidal activity. using the global proteome approach, we report that a ... | 2015 | 25801570 |
preacclimation alters salmonella enteritidis surface properties and its initial attachment to food contact surfaces. | exposure of salmonella to environmental stress, prior to its adherence to a food contact surface, may change the cell surface properties and consequently affect its initial attachment and biofilm formation. this study investigated the influence of temperature and ph preacclimation on the initial attachment of salmonella enteritidis to acrylic and stainless steel. besides, changes in physicochemical properties of cells were examined; and their surface attachment was modeled by xdlvo theory. resul ... | 2015 | 25800356 |
rapid real-time pcr methods to distinguish salmonella enteritidis wildtype field isolates from vaccine strains salmovac se/gallivac se and avipro salmonella vac e. | salmonella enterica serovar enteritidis is a major non-typhoid salmonella serovar causing human salmonellosis mainly associated with the consumption of poultry and products thereof. to reduce infections in poultry, s. enteritidis live vaccine strains avipro salmonella vac e and salmovac se/gallivac se have been licensed and used in several countries worldwide. to definitively diagnose a s. enteritidis contamination in vaccinated herds a reliable and fast method for the differentiation between va ... | 2015 | 25794902 |
characterization of indigenous lactobacillus strains for probiotic properties. | probiotics are defined as adequate amount of live microorganisms able to confer health benefits on the host. currently, most commercially available probiotic products in the market belong to genera lactobacillus. traditional dairy products are usually rich source of lactobacillus strains with significant health benefits. in order to evaluate the probiotic potential of these bacteria, it is essential to assess their health benefits, efficacy, and safety. | 2015 | 25793099 |
a molecular epidemiological investigation of multistate outbreaks of salmonella enteritidis from clinical and environmental samples in turkey, 2000-2010. | to analyze interrelated salmonella enteritidis strains isolated in turkey. fifty-five s. enteritidis surveillance strains were isolated from human feces and environmental samples from different regions in turkey between 2000 and 2010. | 2015 | 25790533 |
empirical prediction and validation of antibacterial inhibitory effects of various plant essential oils on common pathogenic bacteria. | in this study, fractional compound composition, antioxidant capacity, and phenolic substance content of 14 plant essential oils-anise (pimpinella anisum), bay leaves (laurus nobilis), cinnamon bark (cinnamomum verum), clove (eugenia caryophyllata), fennel (foeniculum vulgare), hop (humulus lupulus), istanbul oregano (origanum vulgare subsp. hirtum), izmir oregano (origanum onites), mint (mentha piperita), myrtus (myrtus communis), orange peel (citrus sinensis), sage (salvia officinalis), thyme ( ... | 2015 | 25764982 |
[the metabolism of the innate immunity cells in bacterial infections]. | metabolic activity of innate immunity cells infected by various doses of gram-negative (yersinia pseudotuberculosis, salmonella enteritidis) and gram-positive (staphylococcus aureus, listeria monocytogenes) bacteria has been investigated. using various animal models we found that during the initial period (up to 2 days) changes of infection in cellular responses depend on the type of the pathogen. in response to infection caused by gram-negative bacteria predominant neutrophil accumulation in th ... | 2015 | 25762604 |
in vitro antibacterial and chemical properties of essential oils including native plants from brazil against pathogenic and resistant bacteria. | the antimicrobials products from plants have increased in importance due to the therapeutic potential in the treatment of infectious diseases. therefore, we aimed to examine the chemical characterisation (gc-ms) of essential oils (eo) from seven plants and measure antibacterial activities against bacterial strains isolated from clinical human specimens (methicillin-resistant staphylococcus aureus (mrsa) and sensitive (mssa), escherichia coli, pseudomonas aeruginosa, salmonella typhimurium) and f ... | 2015 | 25757433 |
potential probiotic attributes of a new strain of bacillus coagulans cgmcc 9951 isolated from healthy piglet feces. | a new strain of bacillus coagulans cgmcc 9551, which has a broad range of antibacterial activities against six main pathogenic bacteria including escherichia coli o8, staphylococcus aureus, salmonella enterica subsp. enterica serovar enteritidis, streptococcus suis, listeria monocytogenes and pasteurella multocida, was isolated from healthy piglet feces. in adhesion assay, the isolate exhibited a stronger adhesion to pig intestinal mucus than that of b. subtilis jt143 and l. acidophilus ly24 res ... | 2015 | 25752235 |
a highly sensitive elisa and immunochromatographic strip for the detection of salmonella typhimurium in milk samples. | murine monoclonal antibodies to target salmonella typhimurium flagellin and lipopolysaccharide (lps) were prepared and characterized. for the immunological detection of s. typhimurium, different pairs of monoclonal antibodies (mabs) were tested in a sandwich enzyme linked immunosorbent assay (elisa) format. after comparison, a sandwich elisa and immunochromatographic strip based on lps mabs was established to detect s. typhimurium. the determination limits of the immunochromatographic strip in p ... | 2015 | 25746094 |
an immunosuppressed man with an aortic rupture secondary to salmonella aortitis successfully treated with endovascular aortic repair. | a 75-year-old immunosuppressed man presented with fever and central abdominal pain 3 weeks after having positive blood cultures for salmonella enteritidis. a computed tomography scan demonstrated a contained perforation of a nonaneurysmal abdominal aorta. salmonella aortitis was suspected, and given his recent history of coronary artery stent insertion, endovascular aortic repair was performed, with lifelong antibiotic therapy. at 6 months, there were no remaining clinical or radiological concer ... | 2015 | 25722250 |
evaluation of the effects of a mixture of organic acids and duration of storage on the survival of salmonella on turkey carcasses. | samples from turkey carcasses previously inoculated with salmonella enteritidis 33/66 were subjected to the effect of various mixtures of equal parts of organic acid solutions (acetic, ascorbic, citric, lactic, and tartaric acids). the first part of the study concerned analysis of the influence of the mixtures of organic acids over 15 or 30 min on salmonella enteritidis on turkey carcasses. turkey breast samples were inoculated with salmonella enteritidis at 3.7, 2.7, 1.7, 0.7, and 0.07 log cfu. ... | 2015 | 25719885 |
protection against salmonella typhimurium, salmonella gallinarum, and salmonella enteritidis infection in layer chickens conferred by a live attenuated salmonella typhimurium strain. | in the present study, we investigated the protection conferred by a live attenuated salmonella enterica serovar typhimurium (st) strain against salmonella typhimurium, salmonella gallinarum (sg), and salmonella enteritidis (se) infection in layer chickens. birds were orally primed with the attenuated st strain at 7 days of age and then boosted at 4 weeks post prime immunization (ppi). sequential monitoring of plasma igg and mucosal secretory iga (siga) levels revealed that inoculation with st in ... | 2015 | 25713506 |
in-feed supplementation of trans-cinnamaldehyde reduces layer-chicken egg-borne transmission of salmonella enterica serovar enteritidis. | salmonella enterica serovar enteritidis is a major foodborne pathogen in the united states, causing gastroenteritis in humans, primarily through consumption of contaminated eggs. chickens are the reservoir host of s. enteritidis. in layer hens, s. enteritidis colonizes the intestine and migrates to various organs, including the oviduct, leading to egg contamination. this study investigated the efficacy of in-feed supplementation with trans-cinnamaldehyde (tc), a generally recognized as safe (gra ... | 2015 | 25710365 |
effect of rapid product desiccation or hydration on thermal resistance of salmonella enterica serovar enteritidis pt 30 in wheat flour. | salmonella is able to survive in low-moisture environments and is known to be more heat resistant as product water activity (aw) decreases. however, it is unknown how rapidly the resistance changes if product aw is altered rapidly, as can occur in certain processes. therefore, the objective was to determine the effect of rapid product desiccation or hydration on salmonella thermal resistance. two dynamic moisture treatments were compared with two static moisture treatments to determine the effec ... | 2015 | 25710142 |
comparison of four molecular methods to type salmonella enteritidis strains. | this study compared the pulsed-field gel electrophoresis (pfge), enterobacterial repetitive intergenic consensus-pcr (eric-pcr), multilocus variable-number of tanden-repeat analysis (mlva), and multilocus sequence typing (mlst) methods for typing 188 salmonella enteritidis strains from different sources isolated over a 24-year period in brazil. pfge and eric-pcr were more efficient than mlva for subtyping the strains. however, mlva provided additional epidemiological information for those strain ... | 2015 | 25703542 |
multi locus variable-number tandem repeat (mlva) typing tools improved the surveillance of salmonella enteritidis: a 6 years retrospective study. | surveillance of salmonella enterica subsp. enterica serovar enteritidis is generally considered to benefit from molecular techniques like multiple-locus variable-number of tandem repeats analysis (mlva), which allow early detection and confinement of outbreaks. here, a surveillance study, including phage typing, antimicrobial susceptibility testing and mlva on 1,535 s. enteritidis isolates collected between 2007 and 2012, was used to evaluate the added value of mlva for public health surveillanc ... | 2015 | 25693200 |
high occurrence of extended-spectrum β-lactamase-producing salmonella in broiler carcasses from poultry slaughterhouses in south korea. | the emergence of antibiotic-resistant foodborne salmonella has become a major public health problem. consumption of undercooked poultry contaminated with salmonella can induce food poisoning in humans. in this study, we investigated the occurrence and antibiotic resistance patterns of salmonella spp. isolated from 120 chicken carcasses produced in 6 poultry slaughterhouses in south korea. a total of 11 samples (9.2%) were found contaminated with salmonella: 5 isolates were serotyped as salmonell ... | 2015 | 25692653 |
electrochemical deoxyribonucleic acid biosensor based on electrodeposited graphene and nickel oxide nanoparticle modified electrode for the detection of salmonella enteritidis gene sequence. | in this paper a new electrochemical dna biosensor was prepared by using graphene (gr) and nickel oxide (nio) nanocomposite modified carbon ionic liquid electrode (cile) as the substrate electrode. gr and nio nanoparticles were electrodeposited on the cile surface step-by-step to get the nanocomposite. due to the strong affinity of nio with phosphate groups of ssdna, oligonucleotide probe with a terminal 5'-phosphate group could be attached on the surface of nio/gr/cile, which could further hybri ... | 2015 | 25686924 |
evolution of antimicrobial resistance of salmonella enteritidis (1972-2005). | with the extensive use of antibiotics in livestock production, surveillance revealed an increase in salmonella resistance to the commonly used antimicrobials in veterinary and public health. this serious threat to health care is further exacerbated by the limited epidemiological information about the common zoonotic agent, salmonella enteritidis, required to determine antibiotic therapy. the aim was to characterise the antimicrobial resistance patterns of s. enteritidis isolates across different ... | 2014 | 25686362 |
cross-protection against salmonella typhimurium infection conferred by a live attenuated salmonella enteritidis vaccine. | in this study, a genetically engineered live attenuated salmonella enteritidis (se) vaccine was evaluated for its ability to protect against salmonella typhimurium (st) infection in chickens. the birds were orally primed with the vaccine on the 1st day of life and given an oral booster at 5 wk of age. control birds were orally inoculated with phosphate-buffered saline. both groups of birds were orally challenged with a virulent st strain at 9 wk of age. compared with the control chickens, the va ... | 2015 | 25673904 |
a food-borne outbreak of gastroenteritis caused by different salmonella serotypes in 2 universities in xiamen, fujian, china, in 2012. | we investigated a diarrhea outbreak in 2 universities to identify the etiological agent responsible, the source of infection, the mode of transmission, and the risk factors. a case-controlled study was conducted using case students and asymptomatic control students who were selected randomly and frequency-matched according to class and age, and the source of food or water intake was investigated. of the total 22,404 students at the universities, 0.25% developed salmonella infections. a total of ... | 2015 | 25672350 |
[detection of enteric pathogen bacteria with the pcr method from mixed fecal specimens of food handlers]. | we developed an assay for rapid, specific detection of shigella, salmonella, and verotoxin-producing escherichia coli o157 using the direct pcr analysis of mixed human fecal specimens. in this study, the sensitivity of the direct pcr assay for 50 mixed human fecal specimen was found to be 1.3, 0.42, and 0.76 colony-forming units/kit for shigella sonnei, salmonella typhimurium, and verotoxin-producing e. coli o157, respectively. we compared the efficiency of the direct pcr method with the convent ... | 2014 | 25672139 |
evaluation of antimicrobial activities of sequential spray applications of decontamination treatments on chicken carcasses. | the objective of this study was to evaluate the effects of sequential applications of ɛ-polylysine (epl) or lauramide arginine ethyl ester (lae) sprays followed by an acidic calcium sulfate (acs) spray on inoculated chicken carcasses to reduce salmonella (salmonella enterica serovars including salmonella typhimurium and salmonella enteritidis) contamination during 6 days of storage (4.4°c). secondly, reductions of the resident microflora were studied on uninoculated chicken carcasses following t ... | 2015 | 25656180 |
community-onset pyomyositis caused by a salmonella enterica serovar enteritidis sequence type 11 strain producing ctx-m-15 extended-spectrum β-lactamase. | we report a case of community-onset pyomyositis due to salmonella enterica serovar enteritidis in south korea. the isolated strain was resistant to extended-spectrum cephalosporins and harbored sequence type 11 coproducing ctx-m-15 extended-spectrum β-lactamase (esbl). physicians should be alert for early diagnosis and appropriate treatment since esbl-producing nontyphoidal salmonella infections are difficult to treat without initiation of appropriate empirical antibiotics. | 2015 | 25653405 |
assessment of anti-salmonella activity of boot dip samples. | the introduction of pathogens from the external environment into poultry houses via the boots of farm workers and visitors presents a significant risk. the use of boot dips containing disinfectant to help prevent this from happening is common practice, but the effectiveness of these boot dips as a preventive measure can vary. the aim of this study was to assess the anti-salmonella activity of boot dips that are being used on poultry farms. boot dip samples were collected from commercial laying h ... | 2015 | 25650744 |
sporadic salmonellosis in lower saxony, germany, 2011-2013: raw ground pork consumption is associated with salmonella typhimurium infections and foreign travel with salmonella enteritidis infections. | to investigate risk factors for sporadic salmonellosis, for each notified case four randomly selected population controls matched for age, sex and geographical region were interviewed via self-administered questionnaire. conditional logistic regression analysis of 285 matched pairs revealed significant associations for raw ground pork consumption [odds ratio (or) 6·0, 95% confidence interval (ci) 1·8-20·1], taking antacids (or 5·8, 95% ci 1·4-24·5), eating meat outside the home (or 5·7, 95% ci 2 ... | 2015 | 25626727 |
salmonella enteritidis infection slows steroidogenesis and impedes cell growth in hen granulosa cells. | infection by salmonella enteritidis (se) causes decreased egg production in laying hens. immunoresponse, steroidogenesis, and cell proliferation by chicken granulosa cells (cgcs) are of particular interest because these changes are involved in follicular growth, atresia, and ovulation. to elucidate the possible mechanisms underlying these changes, transcriptional alterations in cgcs at distinct stages of follicular maturity were studied. luteinizing hormone (lh)-and follicle-stimulating hormone ... | 2014 | 25618994 |
biocontrol of salmonella enteritidis in spiked chicken cuts by lytic bacteriophages φsp-1 and φsp-3. | the ability of host specific bacteriophages φsp-1 and φsp-3 to lyse salmonella in artificially contaminated cuts of pressure cooked chicken meat was evaluated at different temperatures -4 °c, room temperature (28 ± 0.5 °c) and 37 °c applying low and high multiplicity of infection (moi). bacteriophages were able to significantly reduce the bacterial counts at all the temperatures studied. at 4 °c, individual application of φ sp-1 and φ sp-3 resulted in significant drop in bacterial counts (log10 ... | 2015 | 25588852 |
selection for pro-inflammatory mediators produces chickens more resistant to eimeria tenella. | we recently developed a novel selection method based on identification and selection of chickens with an inherently high and low phenotype of pro-inflammatory mediators, including interleukin (il)-6, cxcli2, and ccli2. the resultant high line of chickens is more resistant to salmonella enterica serovar enteritidis (salmonella enteritidis) compared to the low line. in the current study, we sought to determine if the high line birds were also more resistant to the protozoan parasite eimeria tenell ... | 2015 | 25577794 |
bacteriophage cocktail reduces salmonella enterica serovar enteritidis counts in raw and smoked salmon tissues. | the use of bacteriophages for the biocontrol of food-borne pathogens is increasingly gaining acceptance. in this study, the effectiveness of bacteriophages to reduce salmonella enteritidis counts was evaluated in raw and smoked salmon tissues. groups of 25 samples each were contaminated with s. enteritidis, treated with a phage mix and then incubated for ten days at 18 °c and 4 °c. a significant bacterial reduction was obtained on days 3, 6 and 10 in raw salmon samples incubated at 18 °c (from 0 ... | 2015 | 25576418 |
dietary β-galactomannans have beneficial effects on the intestinal morphology of chickens challenged with salmonella enterica serovar enteritidis. | salmonella enterica serovar enteritidis is one of the leading causes of food-borne salmonellosis in humans. poultry is the single largest reservoir, and the consumption of incorrectly processed chicken meat and egg products is the major source of infection. since 2006, the use of antibiotics as growth promoters has been banned in the european union, and the dietary inclusion of β-galactomannans (βgm) has become a promising strategy to control and prevent intestinal infections. the aim of this st ... | 2015 | 25568372 |
administration of a salmonella enteritidis δhilassraflig strain by coarse spray to newly hatched broilers reduces colonization and shedding of a salmonella enteritidis challenge strain. | consumption of contaminated poultry meat is still an important cause of salmonella infections in humans. colonization inhibition (ci) occurs when a live salmonella strain is administered to chickens and subsequently protects against challenge with another salmonella strain belonging to the same serotype. a salmonella enteritidis hilassraflig deletion mutant has previously been proven to reduce colonization and shedding of a wild-type salmonella enteritidis strain in newly hatched broilers after ... | 2015 | 25535402 |
chemical composition and antimicrobial activity of the essential oils of pinus peuce (pinaceae) growing wild in r. macedonia. | the chemical composition and antimicrobial activity of the essential oils isolated from twigs with needles (t+n) and from twigs without needles (t-n) from wild pinus peuce griseb. (pinaceae), from three different locations in r. macedonia, were investigated. essential oil yields of t+n ranged from 7.5 ml/kg to 12.5 ml/kg and for t-n from 13.8 ml/kg to 17.3 ml/kg. gc/fid/ms analysis of the essential oils revealed eighty-four components, representing 93.7-95.7% and 91.2-92.0% of the t+n and t-n oi ... | 2014 | 25532297 |
improved antimicrobial activities of synthetic-hybrid bacteriocins designed from enterocin e50-52 and pediocin pa-1. | two hybrid bacteriocins, enterocin e50-52/pediocin pa-1 (ep) and pediocin pa-1/enterocin e50-52 (pe), were designed by combining the n terminus of enterocin e50-52 and the c terminus of pediocin pa-1 and by combining the c terminus of pediocin pa-1 and the n terminus of enterocin e50-52, respectively. both hybrid bacteriocins showed reduced mics compared to those of their natural counterparts. the mics of hybrid pe and ep were 64- and 32-fold lower, respectively, than the mic of pediocin pa-1 an ... | 2015 | 25527560 |
antimicrobial resistance and extended-spectrum β-lactamases of salmonella enterica serotypes isolated from livestock and processed food in portugal: an update. | as salmonella is a common foodborne pathogen, the present study aimed to determine the distribution of salmonella enterica serotypes isolated during 2011-2012 from poultry, swine, cattle, and processed food in portugal, and to characterize the antimicrobial susceptibility and the extended-spectrum β-lactamases (esbls). results were also compared with data obtained before the implementation of the national control program in poultry and the ban of antimicrobial agents in animal feed in the europe ... | 2015 | 25514213 |
similarities between salmonella enteritidis isolated from humans and captive wild animals in south africa. | salmonella is well recognized as an aetiological agent of gastrointestinal and diarrhoeal disease. salmonella enterica serotype enteritidis (salmonella enteritidis) is one of the commonest serotypes associated with foodborne illness. in south africa, we compared salmonella enteritidis strains isolated from humans with gastroenteritis and strains isolated from captive wild animals, between june 2011 and july 2012. | 2014 | 25500660 |
impact of aeromonas and diarrheagenic escherichia coli screening in patients with diarrhea in paraná, southern brazil. | a wide diversity of bacterial agents may cause diarrhea, presenting challenges to clinical laboratories to define a diagnosis. considering that most stool cultures are negative, we screened stool samples from patients with diarrhea for the presence of 14 bacterial enteropathogens, aiming to establish which of them should be included in routine stool analysis. | 2014 | 25500659 |
essential oils from herbs against foodborne pathogens in chicken sausage. | consumption of chicken meat and its products, especially sausage, have increased in recent years. however, this product is susceptible to microbial contamination during manufacturing, which compromises its shelf life. the flavoring and preservative activities of essential oils (eo) have been recognized and the application of these antimicrobial agents as natural active compounds in food preservation has shown promise. the aim of this study was to evaluate the effect of ocimum basilicum and origa ... | 2015 | 25492235 |
molecular characterization of salmonella enterica serovar enteritidis on retail raw poultry in six provinces and two national cities in china. | one hundred and twenty six salmonella enteritidis isolates recovered from 1152 retail raw poultries were characterized by antimicrobial susceptibility test, pulsed-field gel electrophoresis (pfge), presence of quinolone resistance (qnr) associated genes, class i integron, extended spectrum beta-lactamases (esbls) encoding genes, and mutations in quinolone resistance-determining region (qrdr) of gyra and parc. resistance was most frequently found to nalidixic acid (88.1%), followed by to tetracyc ... | 2015 | 25475269 |
efficacy of neutral electrolyzed water for reducing pathogenic bacteria contaminating shrimp. | pathogenic contamination is a food safety concern. this study was conducted to investigate the efficacy of neutral electrolyzed water (new) in killing pathogens, namely, vibrio parahaemolyticus, vibrio vulnificus, salmonella enteritidis, and escherichia coli in shrimp. pure cultures of each pathogen were submerged separately in new containing five different chlorine concentrations: 10, 30, 50, 70, and 100 ppm. for each concentration, three submersion times were tested: 1, 3, and 5 min. the popul ... | 2014 | 25474069 |
effect of white mustard essential oil on the growth of foodborne pathogens and spoilage microorganisms and the effect of food components on its efficacy. | antimicrobial preservative compounds are added to foods to target specific pathogens and spoilage organisms. white mustard essential oil (wmeo) is an extract that contains 4-hydroxybenzyl isothiocyanate, a compound which has been demonstrated to have antimicrobial activity in limited studies. the objective of this research was to determine the in vitro antimicrobial activity of wmeo against gram-positive and gram-negative spoilage and pathogenic bacteria and determine the effect of food componen ... | 2014 | 25474051 |