Publications

commercial levels of chymosin production by aspergillus.	we have increased the production of bovine chymosin in aspergillus niger var. awamori to more than one gram per liter of secreted authentic enzyme by combining a mutagenesis protocol with a novel robotic screening program. analysis of the superior chymosin producing strains indicated that they have enhanced capabilities to secrete extracellular proteins.	1991	1368725
promotive and inhibitory effects of raw starch adsorbable fragments from pancreatic alpha-amylase on enzymatic digestions of raw starch.	the enzymatically inactive but raw-starch-adsorbable peptide fragments designated as gp-pan p and gp-pan i were obtained from a tryptic digest of heat-inactivated hog pancreatic alpha-amylase. these two glycopeptide fragments were purified with sephadex g-75, deae-sephadex a-50, and hplc and were found to be homogeneous on disc gel electrophoresis. gp-pan p and i had molecular weights of 20,000 and 30,000 with sds-page, carbohydrate contents of 10% and 7%, n-terminal amino acids gly-trp and ala- ...	1991	1368657
specific inhibition by cyclodextrins of raw starch digestion by fungal glucoamylase.	alpha-, beta-, and gamma-cyclodextrins (cds) completely inhibited raw starch digestion by glucoamylase i (ga i, mw 90,000) from aspergillus awamori var. kawachi, and inhibited by 85% the raw starch adsorption of ga i at the cd concentrations of 1-5 mm. cds at 1-5 mm did not inhibit gelatinized starch hydrolysis by ga i, but at the concentration of 50 mm, they inhibited such hydrolysis slightly. ga i was specifically adsorbed onto cd-sepharose 6b, but glucoamylase i' (ga i', mw 73,000), which doe ...	1992	1368209
[role of intramolecular bonds in stability of certain enzymes of the cellulolytic complex].	stability of c1- and c2-cellulases, cx-exo- and cx-endoglucanases and beta-glucosidase of aspergillus awamori was studied as affected by monoatomic aliphatic alcohols --methanol, ethanol, propanol and isopropanol; bi- and triatomic alcohols - ethylene glycol and glycerol, urea as well as detergents of dodecyl sulphate and sodium nonilate. the mentioned enzymes are established to manifest the highest activity in 40-60% glycerol. it is also shown that their stability is changed differently under t ...	1976	1258159
[multiple forms of acid phosphatase of the mould aspergillus awamori str. 22].	by isoelectric focusing and deae-cellulose and sephadex g-150 chromatography it has been shown that extracellular acid phosphatase produced by aspergillus awamori str. 22 exists as a mixture of oligomers of molecular weights ranging from 30,000 to 140,000. with an increase of aggregation of molecules of acid phosphatase the isoelectric point increases from 4.6 to 5.4.	1975	1208425
[effect of amino acids on the hemicellulase synthesis by the fungus aspergillus awamori 16-4e].	the effect of different amino acids on the hemicellulase synthesis by the fungus aspergillus awamori 16-4e was investigated. the favorable influence of beta-alanine on the enzyme synthesis was demonstrated. quantitative changes of beta-alanine and glutamic acid in the cell of asp. awamori 16-4e were followed during the fungal development.	1975	1208421
[the effect of iron concentration on pectin decomposition by aspergillus niger and aspergillus awamori].	the effect of cations fe2+ and fe3+ on the decomposition of apple pectin by the enzymic preparations of asp. niger and asp. awamori has been examined. fe ions have delayed the process of enzymic decomposition of the pectin molecule. in the presence of fe3+ far less amounts of monogalacturonic acid are formed. the presence of fe ions makes the pectin molecule more stable to the effect of pectolytic enzymes.	1975	1208377
[effect of the inoculum on beta-fructofuranosidase synthesis by aspergillus awamori].	the influence of the age and amount of the inoculum on the beta-fructofuranosidase synthesis by aspergillus awamori 16 was studied during submerged cultivation. the level of the synthesis increased significantly when the 36-hour mycelial inoculum was used.	1975	1208374
chromatography of acid proteinases and chymotrypsin on a sorbent containing 2,4-dinitrophenyl residues.	a sorbent obtained by treatment of cyanogen bromide-activated sepharose 4b with mono-n-dnp-hexamethylenediamine has been shown to be effective in the affinity chromatography of pepsin, pepsinogen and acid proteinase from aspergillus awamori. it is considered that 2,4-dinitrophenyl residues of the sorbent interact specifically with the hydrophobic zone of the enzyme, which may belong to the substrate binding site. the chromatography of chymotrypsin on the same sorbent supports this assumption.	1975	1097457
[modification of the carboxyl and amine groups of the cellulolytic enzymes of aspergillus awamori].	the activity and stability of some enzymes of asp. awamori cellulolytic complex were studied as affected by chemical modification of carboxylic groups with n,n'-dicyclohexyl carbodiimide (dccd) and amine groups with glutaric aldehyde. the carboxylic groups are established to be necessary for manifestation of the activities of c1- and c2-cellulases, cx-exo- and cx-endoglucanases. their role is negligible in the action of beta-glucosidase. the activity of individual cellulases was studied as affec ...	1976	1021913
distribution and conformation of crystalline nigeran in hyphal walls of aspergillus niger and aspergillus awamori.	hyphal walls of aspergillus awamori containing increased amount of the alpha-glucan, nigeran, became correspondingly more opaque when viewed in the electron microscope as shadowed preparations. however, increased polymer deposition was not accompanied by any significant change in wall thickness. the nigeran of both a. awamori and aspergillus niger occurred in situ in a crystalline conformation identical to that of single crystals prepared with pure polysaccharide. furthermore, this polymer was t ...	1977	914782
[optimization of immobilization conditions for acid proteinase from aspergillus awamori].	to optimize the immobilization conditions for acid proteinase from aspergillus awamori by covalent binding through glutaraldehyde, experiments were carried out using the box-wilson method. the optimization process was assessed on the basis of absolute activity a, coefficient of activity retention gamma and their product a gamma. the following conditions can be recommended: glutaraldehyde concentration 50--60 mg/g, enzyme concentration not less than 40 mg/g, time of glutaraldehyde treatment 2--2. ...	1978	674118
electron microscopy of some rock phosphate dissolving bacteria and fungi.	bacteria pseudomonas striata, bacillus polymyxa, b. megaterium and b. pulvifaciens, and fungi aspergillus awamori, a. niger and penicillium digitatum dissolve tricalcium phosphate and, much less, mussorie and udaipur rock phosphate. the solubilizing power of fungi was higher than that of bacteria, the highest being with a. awamori and a. niger, and with p. striata. electron microscopy of the various cultures showed an electron-dense layer on the bacterial surface after negative staining. the siz ...	1979	527907
[comparative study of glycosidase from cattle liver and exoglycanase from aspergillus awamori].	anomerities of products were estimated for glucosidases from cattle liver and aspergillus awamori. it was demonstrated that the enzyme from cattle liver is alpha-glucosidase and that from asp. awamori is exogluconase. it was demonstrated that alpha-glucosidase hydrolyzes the c1--o bond in the course of reaction. delta-lactone of gluconic acid is a competitive inhibitor for both enzymes. the secondary kinetic isotope effects for both enzymes were measured. the isotope effect for alpha-glucosidase ...	1978	369619
utilization of agricultural wastes of aspergillus awamori for the production of glucoamylase.	the principal industrial use of glucoamylase is in the production of crystalline d-glucose. aspergillus awamori was used. variations in the specific type of carbohydrate source affected the yield of enzyme markedly. of all the substrates tried, maltose supported satisfactory enzyme yields. sucrose, lactose, fructose, and galactose gave abundant growth which seemed to be necessary but no sufficient condition for maximum yields. the specificity of the carbohydrate structure for gluco-amylase produ ...	1977	333823
[preparation and properties of acid proteinase from aspergillus awamori].	from the culture liquid filtrate of aspergillus awamori 78-2 a preparation of acid proteinase was isolated and its properties were described. this producer was shown to have a peculiar capacity of synthesizing under submerged conditions extracellular acid proteinase as the sole proteolytic enzyme. the preparation of acid proteinase showed a comparatively high hydrolyzing capacity. under optimal conditions the enzyme degraded hemoglobin and albumin almost completely. the possibility of purifying ...	1979	42050
[properties of immobilized acid proteinase from aspergillus awamori].	ph-optimum, thermal stability and storage stability of immobilized acid proteinase from aspergillus awamori obtained by covalent binding on silochrome through glutaraldehyde were studied. acid proteinase immobilization was found to shift towards the acid range by a unit. thermal stability of the immobilized preparation was lower than that of the native enzyme.	1978	31618