Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| detection of african horsesickness virus in infected spleens by a sandwich elisa using two monoclonal antibodies specific for vp7. | a sandwich enzyme-linked immunsorbent assay (elisa) for rapid detection of african horsesickness virus (ahsv) in infected spleens or cell culture supernatant was developed. this method uses two monoclonal antibodies (mabs) which recognize two non-overlapping epitopes of the major core protein (vp7) to coat the solid phase, and one labeled with biotin as second antibody. this elisa was evaluated for its ability to detect ahsv in infected spleens resulting in a sensitivity of 97.4% and a specifici ... | 1992 | 1517353 |
| production and properties of monoclonal antibodies against african horsesickness virus, serotype 3. | four polyethylene glycol-mediated cell fusions yielded a total of 23 monoclonal antibodies (mcabs) specific for african horsesickness virus (ahsv). two recognised the major core structural polypeptide, vp7, while one each was specific for the outer capsid proteins, vp2 and vp5. the remainder co-precipitated both vp2 and vp7. an inhibition elisa and radio-immunoprecipitation revealed two types of co-precipitating mcabs, distinguishable from each other by the different relative amounts of the two ... | 1992 | 1513593 |
| diagnostic methods for african horsesickness virus using monoclonal antibodies to structural and non-structural proteins. | a panel of 32 hybridoma cell lines secreting monoclonal antibodies (mabs) reactive with african horsesickness virus serotype 4 (ahsv-4) has been developed. four of the mabs recognized the major core antigen vp7, twenty recognized the outer capsid protein vp2 and eight reacted with the non-structural protein ns1. with the vp7-specific mabs a rapid and sensitive double antibody sandwich immunoassay has been developed to detect viral antigen in infected vero cells and in spleen tissue from ahsv-inf ... | 1992 | 1481354 |
| african horse sickness in spain. | the aetiology, pathogenesis and epizootiology of african horse sickness (ahs) are reviewed with special reference to recent outbreaks in the iberian peninsula. ahs is a highly fatal insect-borne viral disease of equidae. it is caused by an orbivirus (family reoviridae) and nine serotypes are recognised. outbreaks occurred in central spain in 1987 and in southern regions of the iberian peninsula in 1988, 1989 and 1990. all were associated with serotype 4 of the virus, whereas other occurrences of ... | 1992 | 1481352 |
| electron microscopic evidence for endothelial infection by african horsesickness virus. | 1992 | 1448905 | |
| vectors of african horse sickness in the cape verde islands. | 1992 | 1441165 | |
| expression of the major core antigen vp7 of african horsesickness virus by a recombinant baculovirus and its use as a group-specific diagnostic reagent. | the major core protein, vp7, of african horsesickness virus serotype 4 (ahsv-4), the aetiological agent of a recent outbreak of the disease in southern europe, was expressed in insect cells infected with a recombinant baculovirus containing a cloned copy of the relevant ahsv gene (s7). analyses of its biochemical and antigenic properties confirmed the authenticity of the protein expressed. the high-level expression of vp7 under the control of the strong polyhedrin promoter of autographa californ ... | 1992 | 1378881 |
| prevalence of complement-fixing antibody to the african horse sickness virus in domestic animals in nigeria. | the occurrence of antibodies against the african horse sickness virus was investigated in 246 domestic animals (horses, donkeys, camels, dogs) in various regions of nigeria by means of the complement-fixing rate. 34% of the sera tested were positive: 75% in donkeys, 68% in horses, 19% in camels, and 9% in dogs. among the horses, those of 6 to 15 years of age had higher than average prevalence rates than the other age groups. stallions from the northern regions had higher prevalence rates than ma ... | 1992 | 1340757 |
| preliminary findings for an inactivated african horsesickness vaccine using binary ethyleneimine. | investigation studies on inactivated african horsesickness vaccine using binary ethyleneimine were conducted. the inactivation process of virulent type-9 strain using the above inactivant revealed complete virus inactivation at 18, 48 and 84 h post-treatment with inactivant concentrations of 0.004, 0.003 and 0.002m, respectively, without detection of residual virus. an inactivant concentration of 0.003m is recommended and no changes in viral antigenic properties were noticed in complement fixati ... | 1992 | 1339986 |
| african horse sickness and equine infectious anaemia serology in the gambia. | 1992 | 1339038 | |
| susceptibility of aedes krombeini cell line to some arboviruses. | the susceptibility of the newly established ae. krombeini cell line (nivi-ak-453) to six arboviruses, belonging to four different families, was studied. sindbis (sind), vesicular stomatitis (vsv) chandipura (chp) and african horse sickness (ahs) viruses multiplied in these cultures. a four-to-five-fold increase in the virus titres was observed. the maximum titre of sind, vsv, chp and ahs viruses were observed on 1st, 4th, 3rd and 10th post infection days, respectively. a steady and significant i ... | 1992 | 1335966 |
| evolutionary relationships among the gnat-transmitted orbiviruses that cause african horse sickness, bluetongue, and epizootic hemorrhagic disease as evidenced by their capsid protein sequences. | the amino acid sequences of four major capsid proteins of african horse sickness virus (serotype 4, ahsv-4) have been compared with those of bluetongue virus of sheep. epizootic hemorrhagic disease virus of deer, and the phylogenetic relationships established. complete nucleotide sequence analysis of three rna segments (l2, l3, and m6) of ahsv-4 and their encoded products, vp2, vp3, and vp5, together with previously published data for vp7 (roy et al., 1991), have revealed that of the four capsid ... | 1992 | 1329319 |
| the complete sequence of african horsesickness virus serotype 4 (vaccine strain) rna segment 5 and its predicted polypeptide compared with ns1 of bluetongue virus. | the complete sequence of rna segment 5 of the african horsesickness virus serotype 4 (ahsv-4) vaccine strain was determined from cdna clones inserted into pbr322. the rna is 1751 bp long (m(r) 1.12 x 10(6)) and contains an open reading frame encoding a protein of 548 amino acids (m(r) 63,122) with a net charge of +0.5 at neutral ph. a comparison of the sequence of ahsv-4 segment 5 with that of segment 6 of bluetongue virus (btv) serotypes 10 and 17 revealed 49.2% and 48.9% nucleotide similarity, ... | 1992 | 1328499 |
| comparison of the major structural core proteins of tick-borne and culicoides-borne orbiviruses. | comparison of sequence data for broadhaven (brd) virus, a tick-borne orbivirus, and bluetongue virus (btv), the type species of the genus, indicated that rna segments 2 and 7 of brd virus encode the two structural core proteins, vp2 and vp7, respectively. segment 2 is 2792 nucleotides in length with a coding capacity for a protein (vp2) of 908 amino acids and a net charge of +8.5 at neutral ph. segment 7 is 1174 nucleotides in length with a coding capacity for a protein (vp7) of 356 amino acids ... | 1992 | 1328474 |
| characterization of the genes encoding two of the major capsid proteins of epizootic haemorrhagic disease virus indicates a close genetic relationship to bluetongue virus. | the sequences of the genes of two of the major capsid proteins of epizootic haemorrhagic disease virus serotype 1 (ehdv-1, orbivirus genus, reoviridae) have been determined by analyses of cdna clones representing the l2 and s7 rna segments. the ehdv-1 s7 rna segment, which encodes the vp7 core protein, is 1162 nucleotides in length and has the capacity to encode 349 amino acids (m(r) 38,243). the ehdv-1 l2 rna segment, which encodes the outer capsid vp2 protein (m(r) 113,249) is 2968 nucleotides ... | 1992 | 1321879 |
| letter: ahs vaccine. | 1976 | 1258291 | |
| the multiplication of african horse-sickness virus in two species of culicoides (diptera, ceratopogonidae). | type 9 african horse-sickness virus multiplied to a high titre in both culicoides nubeculosus and c. variipennis after intrathoracic inoculation and in c. variipennis after oral ingestion. the orally infected c. variipennis were able to transmit the virus by biting after 13 days incubation at 26 degrees c but not after 6 days incubation. intrathoracically inoculated c. variipennis were able to transmit the virus after 4 days incubation. it is suggested that c. variipennis can act as a biological ... | 1975 | 1169931 |
| the growth of african horse-sickness virus in embryonated hen eggs and the transmission of virus by culicoides variipennis coquillett (diptera, ceratopogonidae). | seven-day-old embryonated hen eggs were infected with african horse sickness virus by the yolk sac and intravenous routes. virus reached a high titre in the blood of infected embryos. culicoides variipennis midges which took a blood meal from infected eggs became infected with virus, and after 7 days at 26 degrees - 27 degrees c transmitted african horse sickness virus to uninfected eggs. c. variipennis may therefore be considered a biological vector of african horse sickness virus in the labora ... | 1975 | 1169930 |
| a gel electrophoretic study of the protein and nucleic acid components of african horsesickness virus. | the physico-chemical structure of african horsesickness virus (ahsv) is compared with that of some of the other members of the reoviridae, and in particular with that of bluetongue virus (btv), the type strain of the orbivirus genus. this study adduces evidence of a great similarity between the gel electrophoretic patterns of the polypeptides of ahsv and btv. the molecular mass values of the 7 ahsv polypeptides range between 0,30 x 10(5) and 1,46 x 10(5) dalton, a variation similar to that of bt ... | 1976 | 1023091 |
| african horse sickness virus antibodies in northern nigeria 1974--1975. | 1977 | 877421 | |
| elephants and zebras as possible reservoir hosts for african horse sickness virus. | 1977 | 860390 | |
| inactivation of african horse-sickness virus by betapropiolactone and by ph. | the inactivation of several types of african horse sickness virus (ahsv) by ph and by betapropiolactone (bpl) was studied. at 19 degrees - 22 degrees c the virus was stable between ph 6.0 and 10.4, whether suspended in mouse brain or in serumfree buffer. below ph 5.6 and above ph 10.9, more than 99 per cent of infectivity was inactivated within 15 minutes. the addition of 50 per cent serum did not influence ph stability. disinfection in the presence of citric acid and caustic soda is briefly dis ... | 1975 | 237497 |
| characterization of the tubules associated with the replication of three different orbiviruses. | 1979 | 218352 | |
| rates of infection in, and transmission of, african horse-sickness virus by aedes aegypti mosquitoes. | very low infection rates (less than 3%) were obtained when aedes aegypti mosquitoes ingested blood contained 5.8--6.5 log10 mld50/0.02 ml african horse sickness virus (ahsv). when a. aegypti mosquitoes were inoculated intrathoracically with virus, however, high infection rates were achieved. mosquitoes infected by inoculum failed to transmit virus to embryonated hens eggs by bite, and virus could not be detected in membrane or blood when inoculated mosquitoes were allowed to engorge on uninfecte ... | 1978 | 29475 |