Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| a kshv encoded ortholog of mir-155 induces human splenic b-cell expansion in nod/ltsz-scid il2r{gamma}null mice. | micrornas are small non-coding rna molecules that function as post-transcriptional regulators of gene expression. kaposi's sarcoma-associated herpesvirus (kshv), a b-cell-tropic virus associated with kaposi's sarcoma (ks) and b-cell lymphomas, encodes 12 mirna genes that are highly expressed in these tumor cells. one viral mirna, mir-k12-11, shares 100% seed sequence homology with hsa-mir-155, an oncogenic human mirna that functions as a key regulator of hematopoiesis and b-cell differentiation. ... | 2011 | 21813606 |
| replication of recombinant herpesvirus of turkey expressing genes of infectious laryngotracheitis virus in specific pathogen free and broiler chickens following in ovo and subcutaneous vaccination. | replication of a recombinant herpesvirus of turkey vaccine expressing infectious laryngotracheitis virus genes (rhvt-lt) was evaluated in specific pathogen free (spf) and commercial broiler chickens after various vaccination protocols (amniotic route at embryonation day [ed] 18; intra-embryonic route at ed 19; and subcutaneous at 1 day of age [s.c.]). three experiments were conducted: in the first experiment, replication of rhvt-lt vaccine was chronologically evaluated and compared with the repl ... | 2011 | 21812719 |
| large b cell lymphomas with plasmablastic differentiation: a biological and therapeutic challenge. | abstract plasmablastic differentiation can be found in a variety of large b cell lymphomas, including plasmablastic lymphoma, alk-positive large b cell lymphoma, primary effusion lymphoma, large b cell lymphoma arising in hhv-8-associated multicentric castleman disease and diffuse large b cell lymphoma with partial plasmablastic phenotype. these tumors are characterized by the acquisition of the transcriptional profile of plasma cells (with overexpression of prdm1/blimp1 and xbp1s, in concert wi ... | 2011 | 21812534 |
| multiplex pcr for six herpesviruses after hematopoietic stem cell transplantation. | background. herpesviruses cause life-threatening diseases after hematopoietic stem cell transplantation (hsct). it is necessary that viral diseases are identified early and safely diagnosed. the purpose of this study was to evaluate the efficacy of multiplex polymerase chain reaction (pcr) for qualitative detection of the six herpesviruses simultaneously: herpes simplex virus type 1 and type 2, varicella-zoster virus, cytomegalovirus (cmv), epstein-barr virus (ebv) and human herpesvirus 6b (hhv- ... | 2011 | 21810148 |
| simultaneous monitoring of cmv and human herpesvirus 6 infections and diseases in liver transplant patients: one-year follow-up. | the aim of this study was to simultaneously monitoring cytomegalovirus and human herpesvirus 6 active infections using nested-polymerase chain reaction and, together with clinical findings, follow the clinical status of patients undergoing liver transplant. | 2011 | 21808857 |
| lymphotropic herpesvirus dna detection in patients with active cmv infection - a possible role in the course of cmv infection after hematopoietic stem cell transplantation. | background: the natural history of cytomegalovirus (cmv) infection and disease in transplant recipients prompts researchers to look for other factors contributing to this infection. the ubiquity of lymphotropic herpesviruses (ebv, hhv-6, and hhv-7) and the possibility of their activation during immunosuppression may suggest their participation in progression of cmv infection in patients after hematopoietic stem cell transplantation (hsct).<br /> material/methods: the presence of cmv, ebv, hhv-6 ... | 2011 | 21804462 |
| an equine herpesvirus 1 (ehv-1) vectored h1 vaccine protects against challenge with swine-origin influenza virus h1n1. | in 2009, a novel swine-origin h1n1 influenza a virus (s-oiv), antigenically and genetically divergent from seasonal h1n1, caused a flu pandemic in humans. development of an effective vaccine to limit transmission of s-oiv in animal reservoir hosts and from reservoir hosts to humans and animals is necessary. in the present study, we constructed and evaluated a vectored vaccine expressing the h1 hemagglutinin of a recent s-oiv isolate using equine herpesvirus 1 (ehv-1) as the delivery vehicle. exp ... | 2011 | 21803510 |
| complete genome sequence of virulent duck enteritis virus (dev) strain 2085 and comparison with genome sequences of virulent and attenuated dev strains. | we here report the complete genome sequence of the duck enteritis virus (dev) wild-type strain 2085, an avian herpesvirus (genbank id: jf999965). the nucleotide sequence was derived from the 2085 genome cloned as an infectious bacterial artificial chromosome (bac) clone. the dev 2085 genome is 160,649-bp in length and encodes 78 predicted open reading frames (orfs), a number identical to that identified for the attenuated dev vac strain (genbank id: eu082088.2). comparison of the genome sequence ... | 2011 | 21802458 |
| a fatal case of human herpesvirus 6 chronic myocarditis in an immunocompetent adult. | background: human herpesvirus 6 (hhv-6) is an important cause of fulminant or acute viral myocarditis in immunocompromised or immunocompetent patients. however the physiopathological mechanisms of hhv-6 related acute myocarditis and the involvement of subsequent hhv-6 reactivation phases in the development of chronic cardiomyopathies remain to be assessed. objectives: to describe a case of fatal hhv-6 chronic myocarditis in an immunocompetent adult. study design: case report and detailed histolo ... | 2011 | 21802354 |
| the complex relationship between human herpes virus 6 and acute graft-versus-host disease. | the most frequent manifestation of human herpes virus 6 (hhv6) reactivation following allogeneic hsct is a febrile rash, thus questioning its relationship with graft-versus-host disease (gvhd). in this retrospective analysis of 365 patients who underwent allogeneic hsct, hhv6 reactivation was significantly associated with cord blood transplantation (hazard ratio (hr), 3.20; p<0.0001) and the use of unrelated donors (hr, 2.02 p=0.008). by multivariate analysis previous gvhd was a predictive facto ... | 2011 | 21801705 |
| surveillance of aseptic central nervous system infections in poland: is it meeting its objectives? | 2011 | 21801691 | |
| virus reactivation: a panoramic view in human infections. | viruses are obligate intracellular parasites, relying to a major extent on the host cell for replication. an active replication of the viral genome results in a lytic infection characterized by the release of new progeny virus particles, often upon the lysis of the host cell. another mode of virus infection is the latent phase, where the virus is 'quiescent' (a state in which the virus is not replicating). a combination of these stages, where virus replication involves stages of both silent and ... | 2011 | 21799704 |
| deletion of kaposi's sarcoma herpesvirus flice inhibitory protein (vflip) from the viral genome compromises the activation of stat-1 responsive cellular genes and spindle cell formation in endothelial cells. | kaposi's sarcoma herpesvirus (kshv) flice inhibitory protein, vflip, has anti-apoptotic properties, is a potent activator of the nf?b pathway and induces the formation of endothelial spindle cells, the hallmark of kaposi's sarcoma, when overexpressed in primary endothelial cells. we used a reverse genetics approach to study several functions of the kshv vflip in the context of the whole viral genome. deletion of the gene encoding vflip from a kshv genome cloned in a bacterial artificial chromoso ... | 2011 | 21795355 |
| infection of lymphoblastoid cell lines by kshv: critical role of cell-associated virus. | kaposi's sarcoma-associated herpesvirus (kshv) displays strong lymphotropism in vivo, but paradoxically, established b cell lines have largely been refractory to infection by soluble kshv virions. here we show that this block can be overcome by exposure to cell-associated virus. doxycycline-inducible rkshv.219-harboring slk cells (islk.219) were employed as kshv donors. co-cultivation of lymphoid cell lines with reactivated islk.219 cells resulted in readily demonstrable viral entry into each ce ... | 2011 | 21795352 |
| construction of a lytically replicating kaposi's sarcoma-associated herpesvirus (kshv). | kshv is found predominantly in a latent state in most cell types, impeding investigations of the lytic replication cycle. here we engineered the cloned kshv genome, bac36, to enforce constitutive expression of the main lytic switch regulator, rta (orf50). the resulting virus, kshv-lyt, activated by default the lytic cycle and replicated to high titers in various cells. using kshv-lyt we showed that orf33 (encoding a tegument protein) is essential for lytic kshv replication in cell culture, but o ... | 2011 | 21795351 |
| hvem and nectin-1 mediate hsv-1 infection of the murine cornea. | herpes simplex virus type 1 (hsv-1) is a ubiquitous human pathogen that enters cells by receptor mediated fusion of the viral envelope with a host cell membrane. the envelope glycoprotein gd of hsv must bind to one of its receptors for entry to take place. recent studies using knock-out mice demonstrated that the gd receptors, herpes virus entry mediator (hvem) and nectin-1, are the primary entry receptors for hsv-2 in the mouse vagina and brain. nectin-1 was most crucial for neuronal spread of ... | 2011 | 21795335 |
| identification of functional domains of the ir2 protein of equine herpesvirus 1 required for inhibition of viral gene expression and replication. | the equine herpesvirus 1 (ehv-1) negative regulatory ir2 protein (ir2p), an early 1,165-amino acid (aa) truncated form of the 1487-aa immediate-early protein (iep), lacks the trans-activation domain essential for iep activation functions but retains domains for binding dna, tfiib, and tbp and the nuclear localization signal. ir2p mutants of the n-terminal region which lack either dna-binding activity or tfiib-binding activity were unable to down-regulate ehv-1 promoters. in ehv-1-infected cells ... | 2011 | 21794889 |
| safety of pre-engraftment prophylactic foscarnet administration after allogeneic stem cell transplantation. | k. ishiyama, t. katagiri, k. ohata, k. hosokawa, y. kondo, h. yamazaki, a. takami, s. nakao. safety of pre-engraftment prophylactic foscarnet administration after allogeneic stem cell transplantation. transpl infect dis 2011. all rights reserved abstract: human herpesvirus-6 (hhv-6) is a major cause of limbic encephalitis with a dismal prognosis after allogeneic hematopoietic stem cell transplantation (sct). because our previous trial of preemptive therapy with foscarnet sodium (phosphonoformic ... | 2011 | 21794043 |
| effect of diluting marek's disease vaccines on the outcomes of marek's disease virus infection when challenged with highly virulent marek's disease viruses. | dilution of marek's disease (md) vaccines is a common practice in the field to reduce the cost associated with vaccination. in this study we have evaluated the effect of diluting md vaccines on the protection against md, vaccine and challenge md virus (mdv) kinetics, and body weight when challenged with strains md5 (very virulent mdv) and 648a (very virulent plus mdv) by contact at day of age. the following four vaccination protocols were evaluated in meat-type chickens: turkey herpesvirus (hvt) ... | 2011 | 21793444 |
| bilateral tonsillar and esophageal kaposi sarcoma in an hiv-negative patient. | tonsillar involvement in kaposi sarcoma is extremely rare, as only a few such cases have been reported; all but 1 of these previously reported cases occurred in patients with human immunodeficiency virus (hiv) infection. we describe what to the best of our knowledge is the first reported case of concurrent bilateral tonsillar and esophageal kaposi sarcoma in an hiv-negative patient. a 68-year-old man presented with sore throat and dysphagia. clinical examination revealed the presence of bilatera ... | 2011 | 21792787 |
| newly emerging therapies targeting viral-related lymphomas. | gamma-(?)-herpes virus lymphomas comprise a heterogenous group of b-cell and t-cell neoplasms most commonly associated with epstein-barr virus and rarely human herpes virus-8 infection. adult t-cell leukemia/lymphoma (atll) is a unique disease entity caused by the human t-cell lymphotrophic virus, type 1 (htlv-i), the only retrovirus known to cause cancer in humans. viral lymphomas behave aggressively and disproportionally affect immunocompromised individuals and those living in underdeveloped r ... | 2011 | 21792540 |
| bortezomib-induced epstein-barr virus and kaposi sarcoma herpesvirus lytic gene expression: oncolytic strategies. | purpose of review: gamma herpesviruses (ghvs) are responsible for a substantial proportion of virus-associated human cancers, particularly in immunocompromised individuals. methods that employ lytic activation of viruses latently infecting tumors represent a novel strategy of antineoplastic therapy. recent findings: the proteasome inhibitor, bortezomib, has been shown to be a potent activator of ghv lytic cycle and has demonstrated activity in case reports of ghv-related malignancies. although i ... | 2011 | 21788895 |
| cyclopropavir inhibits the normal function of the human cytomegalovirus ul97 kinase. | cyclopropavir (cpv) is active against human cytomegalovirus (cmv) as well as both variants of human herpes virus 6 and human herpesvirus 8. the mechanism of action of cpv against cmv is similar to that of ganciclovir (gcv) in that it is phosphorylated initially by the cmv ul97 kinase resulting in inhibition of viral dna synthesis. resistance to cpv maps to the ul97 kinase, but is associated primarily with h520q mutations and thus retains good antiviral activity against most gcv-resistant isolate ... | 2011 | 21788463 |
| estimation of the diagnostic accuracy of the glyco-c and us9 gene-based polymerase chain reaction technique for the detection of bovine herpesvirus type 5 dna in decomposed brain suspension from a slaughter house using bayesian analysis, brazil. | brazil represents the greatest beef producer among tropical countries, and the major obstacle for meat international trade is sanitary problems especially closely related to viral encephalitis. the goal of this study was to estimate the accuracy of the glycol and us9 gene-based polymerase chain reactions (pcrs) for the detection of bovine herpesvirus type 5 (bohv-5) from decomposed brain samples (n?=?95). for this purpose, a latent-class (bayesian) approach was used. sensitivity (se) was estimat ... | 2011 | 21786161 |
| deciphering the clinical impact of acute human herpesvirus 6 (hhv-6) infections. | human herpesvirus 6 (hhv-6) is a ubiquitous virus inducing a life-long latent infection of its human host. acute infections (ais) have been recognized as the cause of severe diseases. these ais correspond to primary infections (pis), mainly occurring in young children, endogenous reactivations (enrs), observed at any age, and putative exogenous reinfections (exrs). the diagnosis of ais is now essentially based on the quantification of viral load in bodily fluids and organs by means of real time ... | 2011 | 21782505 |
| identification and isolation of psittacid herpesvirus from psittacids in brazil. | psittacid herpesvirus (pshv) was isolated from 41 birds kept in captivity in belo horizonte, minas gerais/brazil using chicken embryo fibroblasts (cef) cell cultures. for this study, leukocytes or cloacal swabs of live birds were used. also, portions of liver, spleen or kidney from birds collected at necropsy were utilized for these tests. pcr tests confirmed the presence of pshv in 100% of samples. thirty-three of the pcr products were sequenced and the results disclosed a 99% and 100% identity ... | 2011 | 21782357 |
| phylogenetic analysis of suid herpesvirus 1 isolates from argentina. | argentinean suid herpesvirus 1 isolates were compared with reference strains and sequences available at genbank and phylogenetically analyzed. a short fragment of the ge gene of the immunodominant epitopes was used for preliminary grouping of isolates by phylogenetic analysis. the analysis of the partial gc gene provided more precise genetic typing and segregation into the main genotypes i and ii. results confirmed that the argentinean genotype i isolates predominate in our country. the topology ... | 2011 | 21782355 |
| early-life environment influencing susceptibility to cytomegalovirus infection: evidence from the leiden longevity study and the longitudinal study of aging danish twins. | summaryhuman cytomegalovirus (cmv) is a common herpesvirus establishing lifelong persisting infection, which has been implicated in immunosenescence and mortality in the elderly. little is known about how and when susceptibility to cmv infection is determined. we measured cmv seroprevalence in two genetically informative cohorts. from the leiden longevity study (lls) we selected long-lived sib-pairs (n=844) and their middle-aged offspring and the offspring's partners (n=1452). from the longitudi ... | 2011 | 21781370 |
| b and t lymphocyte attenuator regulates the development of antigen-induced experimental conjunctivitis. | purpose: to investigate the roles that b and t lymphocyte attenuator (btla) and herpesvirus entry mediator (hvem) play in the development of antigen-induced experimental conjunctivitis (ec). methods: balb/c mice were immunized with ragweed (rw) in alum. ten days later, the mice were challenged with rw in eye drops. after 24 hours, the conjunctivas, blood and spleens were collected for histological analysis, measurement of serum immunoglobulin (ig) levels, and both flow cytometric analysis and cy ... | 2011 | 21779950 |
| evasion and subversion of interferon-mediated antiviral immunity by kaposi's sarcoma-associated herpesvirus: an overview. | viral invasion of a host cell triggers immune responses consisting of both the innate and adaptive components. the innate immune response involving induction of type i inferferons (ifn a and ß) constitutes the first line of antiviral defenses. the type i ifns signal the transcription of a group of anti-viral effector proteins, the ifn stimulated genes (isgs), which target distinct viral components and distinct stages of the viral life cycle, aiming to eliminate invading viruses. in case of kapos ... | 2011 | 21775463 |
| development of a sandwich elisa for the detection of bovine herpesvirus type 1. | to develop a standard enzyme-linked immunosorbent assay (elisa) for the detection of bovine herpesvirus type 1 (bhv-1). | 2011 | 21771677 |
| herpesvirus saimiri-mediated delivery of the adenomatous polyposis coli tumour suppressor gene reduces proliferation of colorectal cancer cells. | colorectal cancer (crc) is a major cause of cancer-related mortality. a contributing factor to the progression of this disease is sporadic or hereditary mutation of the adenomatous polyposis coli (apc) gene, a negative regulator of the wnt signalling pathway. inherited mutations in apc cause the disorder familial adenomatous polyposis (fap), which leads to crc development in early adulthood. however, the gene is also disrupted in some 60% of sporadic cancers. restoration of functional apc may sl ... | 2011 | 21769429 |
| intrathecal human herpesvirus 6 antibodies in multiple sclerosis and other demyelinating diseases presenting as oligoclonal bands in cerebrospinal fluid. | demyelinating diseases of the central nervous system (cns) often include elevated igg production in intrathecal space presenting as oligoclonal bands (ocbs) in cerebrospinal fluid (csf). in most demyelinating diseases, e.g. in multiple sclerosis (ms), the underlying cause is not known. we used isoelectric focusing and affinity immunoblot to study the specificity of csf ocbs to human herpesvirus-6 (hhv-6) in patients with demyelinating diseases of the cns including ms. eighty patients with positi ... | 2011 | 21767883 |
| the membrane-bound transcription factor creb3l1 is activated in response to virus infection to inhibit proliferation of virus-infected cells. | creb3l1/oasis is a cellular transcription factor synthesized as a membrane-bound precursor and activated by regulated intramembrane proteolysis in response to stimuli like er stress. comparing gene expression between huh7 subclones that are permissive for hepatitis c virus (hcv) replication versus the nonpermissive parental huh7 cells, we identified creb3l1 as a host factor that inhibits proliferation of virus-infected cells. upon infection with diverse dna and rna viruses, including murine ?-he ... | 2011 | 21767813 |
| viral interleukin-6: role in kaposi's sarcoma-associated herpesvirus-associated malignancies. | viral interleukin-6 (vil-6) is a product of kaposi's sarcoma-associated herpesvirus (kshv) expressed in latently infected cells and to a higher degree during viral replication. a distinctive feature of vil-6 is the ability to directly bind and activate gp130 signaling in the absence of other receptor subunits. secretion of vil-6 is generally poor, but vil-6 can activate gp130 from inside the cell. due to the wide cell distribution of gp130, vil-6 has the potential to induce a wide range of biolo ... | 2011 | 21767154 |
| how the virus outsmarts the host: function and structure of cytomegalovirus mhc-i-like molecules in the evasion of natural killer cell surveillance. | natural killer (nk) cells provide an initial host immune response to infection by many viral pathogens. consequently, the viruses have evolved mechanisms to attenuate the host response, leading to improved viral fitness. one mechanism employed by members of the ß-herpesvirus family, which includes the cytomegaloviruses, is to modulate the expression of cell surface ligands recognized by nk cell activation molecules. a novel set of cytomegalovirus (cmv) genes, exemplified by the mouse m145 family ... | 2011 | 21765638 |
| infection of peripheral blood mononuclear cells with neuropathogenic equine herpesvirus type-1 strain ab4 reveals intact interferon-a induction and induces suppression of anti-inflammatory interleukin-10 responses in comparison to other viral strains. | the recent increase in incidence, morbidity, and mortality of neurological disease induced by equine herpesvirus type 1 (ehv-1) has suggested a change of virulence of the virus. the exact mechanisms by which ehv-1 induces neurologic disease are not known. environmental, viral, and host risk factors might contribute to neurological manifestation. here, we investigated innate interferon-a (ifn-a), interleukin-10 (il-10) and il-4 responses after infection of equine peripheral blood mononuclear cell ... | 2011 | 21764140 |
| structural and functional analysis of the tap-inhibiting ul49.5 proteins of varicelloviruses. | viral infections are counteracted by virus-specific cytotoxic t cells that recognize the infected cell via mhc class i (mhc i) molecules presenting virus-derived peptides. the loading of the peptides onto mhc i molecules occurs in the endoplasmic reticulum (er) and is facilitated by the peptide loading complex. a key player in this complex is the transporter associated with antigen processing (tap), which translocates the viral peptides from the cytosol into the er. herpesviruses have developed ... | 2011 | 21764135 |
| cd3?-independent pathways in tcr-mediated signaling in mature t and inkt lymphocytes. | antigen recognition by t-lymphocytes through the t-cell antigen receptor, tcr-cd3, is a central event in the initiation of an immune response. cd3 proteins may have redundant as well as specific contributions to the intracellular propagation of tcr-mediated signals. however, to date, the relative role that each cd3 chain plays in signaling is controversial. in order to examine the roles of cd3? chain in tcr signaling, we analyzed proximal and distal signaling events in human cd3?(-/-) primary an ... | 2011 | 21764047 |
| human immunodeficiency virus type 1 induces lytic cycle replication of kaposi's-sarcoma-associated herpesvirus: role of ras/c-raf/mek1/2, pi3k/akt, and nf-?b signaling pathways. | human immunodeficiency virus type 1 (hiv-1) infection significantly increases the risk and development of kaposi's sarcoma (ks) in individuals infected with ks-associated herpesvirus (kshv). previously, we reported that hiv-1 tat protein induced kshv replication by modulating the janus kinase/signal transducers and activators of transcription signaling pathway. here, we further investigated the possible signaling pathways involved in hiv-1-induced reactivation of kshv. we showed that hiv-1 infec ... | 2011 | 21763505 |
| human herpesvirus 1 in wild marmosets, brazil, 2008. | to the editor: human herpesvirus 1 (hhv-1) infections in new world monkey species, especially in the callithrichid family, have been described (1-6), but most reports have discussed experimental infections or isolated spontaneous infections in pet, zoo, or research animals. we report an outbreak of hhv-1 in wild marmosets (callithrix spp.) in the city of rio de janeiro, brazil. | 2011 | 21762598 |
| recurrent pseudo-torch appearences of the brain presenting as "dandy-walker" malformation. | abstract dandy-walker malformation (dwm) is a developmental abnormality characterized by cystic dilatation of the fourth ventricle, complete or partial agenesis of the cerebellar vermis and enlarged posterior fossa with upward displacement of the lateral sinuses, tentorium and torcula. calcification of brain tissue is a feature of congenital infection, particularly those involving the torch group. an autosomal recessive congenital infection-like syndrome with intracranial and extracaranial calci ... | 2011 | 21762029 |
| domain-domain interactions underlying herpesvirus-human protein-protein interaction networks. | protein-domains play an important role in mediating protein-protein interactions. furthermore, the same domain-pairs mediate different interactions in different contexts and in various organisms, and therefore domain-pairs are considered as the building blocks of interactome networks. here we extend these principles to the host-virus interface and find the domain-pairs that potentially mediate human-herpesvirus interactions. notably, we find that the same domain-pairs used by other organisms for ... | 2011 | 21760902 |
| hiv-associated multicentric castleman disease. | purpose of review: hiv-associated multicentric castleman disease (hiv mcd) is a rare lymphoproliferative disorder, the incidence of which appears to be increasing in the highly active antiretroviral therapy era. current knowledge of the disease is limited and this review will discuss what is known about the pathophysiology, diagnosis, management, and prognosis of hiv mcd. recent findings: hiv mcd has been shown to be associated with infection with human herpesvirus-8. vascular endothelial growth ... | 2011 | 21760504 |
| plasmablastic lymphoma and related disorders. | b-cell lymphomas with plasmablastic features are a heterogeneous group of lymphomas. while they may share overlapping morphologic or immunophenotypic features, distinct clinicopathologic or molecular genetic features exist for some that have allowed their recognition as distinct entities. session 2 of the 2009 society for hematopathology/european association for haematopathology workshop dealt with the theme of plasmablastic lymphomas (pbls) and related disorders. topics included human herpesvir ... | 2011 | 21757592 |
| viral mirnas and immune evasion. | viral mirnas, ~22nt rna molecules which post-transcriptionally regulate gene expression, are emerging as important tools in immune evasion. viral infection is a complex process that requires immune evasion in order to establish persistent life-long infection of the host. during this process viruses express both protein-coding and non-coding genes, which help to modulate the cellular environment making it more favorable for infection. in the last decade, it was uncovered that dna viruses express ... | 2011 | 21757042 |
| development of a microwell adapted immunoblot system with recombinant antigens for distinguishing human herpesvirus (hhv)6a and hhv6b and detection of human cytomegalovirus. | abstract background: the human cytomegalovirus (hcmv) and the human herpesvirus 6 (hhv6) are widely distributed in the human population. the variants a and b of hhv6 are closely related to each other and cannot be distinguished by common serological methods like enzyme-linked immunosorbent assay (elisa) or immunofluorescence test (ift). the aim of this study was to develop a microwell-adapted blot system for specificity detection of human cytomegalovirus and human herpesvirus 6a and 6b (hhv6a, h ... | 2011 | 21756162 |
| enterovirus and herpesviridae family as etiologic agents of lymphomonocytary meningitis, southern brazil. | viral meningitis is a common infectious disease of the central nervous system (cns) that occurs worldwide. the aim of this study was to identify the etiologic agent of lymphomonocytary meningitis in curitiba, pr, brazil. during the period of july 2005 to december 2006, 460 cerebrospinal fluid (csf) samples with lymphomonocytary meningitis were analyzed by pcr methodologies. fifty nine (12.8%) samples were positive. enteroviruses was present in 49 (83%) samples and herpes virus family in 10 (17%) ... | 2011 | 21755125 |
| kaposi sarcoma of the stomach: a case report. | a 60-year-old man presented with persistent dysphagia and weight loss of 2-months duration. an upper gi endoscopy revealed mycotic oesophagitis and chronic gastritis with two ulcers of the gastric body and antrum. repeat endoscopy was performed after medical treatment failed, and histological examination on new biopsy samples led to a diagnosis of kaposi sarcoma of the stomach. hiv infection was not known at this time; however, the patient was tested after the diagnosis of kaposi sarcoma was mad ... | 2009 | 21754959 |
| chemotactic antiviral cytokines promote infectious apical entry of human adenovirus into polarized epithelial cells. | mucosal epithelia provide strong barriers against pathogens. for instance, the outward facing apical membrane of polarized epithelial cells lacks receptors for agents, such as hepatitis c virus, herpesvirus, reovirus, poliovirus or adenovirus. in addition, macrophages eliminate pathogens from the luminal space. here we show that human adenovirus type 5 engages an antiviral immune response to enter polarized epithelial cells. blood-derived macrophages co-cultured apically on polarized epithelial ... | 2011 | 21750545 |
| infection in immunocompromised hosts and the organ transplant recipient -essentials. | 2011 | 21748845 | |
| ccaat/enhancer binding proteins play a role in orilyt-dependent genome replication during mhv-68 de novo infection. | murine gammaherpesvirus 68 (mhv-68), a member of the gammaherpesvirus family, replicates robustly in permissive cell lines and is able to infect laboratory mice. mhv-68 has emerged as a model for studying the basic aspects of viral replication and host-virus interactions of its human counterparts. herpesvirus genome replication is mediated through a cis-element in the viral genome called the origin of lytic replication (orilyt). a family of transcription factors, ccaat/enhancer binding proteins ... | 2011 | 21748596 |
| quantitative proteomic analyses of human cytomegalovirus-induced restructuring of endoplasmic reticulum-mitochondrial contacts at late times of infection. | endoplasmic reticulum-mitochondrial contacts, known as mitochondria-associated membranes, regulate important cellular functions including calcium signaling, bioenergetics, and apoptosis. human cytomegalovirus is a medically important herpesvirus whose growth increases energy demand and depends upon continued cell survival. to gain insight into how human cytomegalovirus infection affects endoplasmic reticulum-mitochondrial contacts, we undertook quantitative proteomics of mitochondria-associated ... | 2011 | 21742798 |
| induction of cd56(+) t cells after prolonged activation of t cells in vitro: a possible mechanism for cd4(+) t-cell depletion in acquired immune deficiency syndrome patients. | the pathogenic mechanisms responsible for depletion of cd4(+) t cells in aquired immune deficiency syndrome (aids) are not fully understood. systemic immune activation mediated by persistent infection of human immunodeficiency virus (hiv) seems to be one of the predictors of disease progression. we predicted that certain lymphocytes responsible for cd4(+) t-cell depletion could be induced in patients during prolonged activation of lymphocytes. therefore, we have established an in vitro long-term ... | 2011 | 21741423 |
| kshv regulation of fibulin-2 in kaposi's sarcoma implications for tumorigenesis. | kaposi's sarcoma is an angioproliferative tumor caused by kaposi's sarcoma-associated herpesvirus (kshv) infection of vascular endothelial cells. fibulins, proteins that associate with extracellular matrix (ecm) proteins, may have both tumor-suppressive and oncogenic activities. we found that the expression of fibulin-2 protein and mrna were decreased 50-fold and 26-fold, respectively, in 10-day kshv-infected dermal microvascular endothelial cells (dmvec). using quantitative rt-pcr, we found a f ... | 2011 | 21741351 |
| kinetics and pathogenicity of oral infection by equine herpesvirus-9 in mice and suckling hamsters. | the pathogenesis and kinetics of oral infection by equine herpesvirus (ehv)-9 were studied in mice and hamsters. after oral inoculation of 10(5) plaque-forming units (pfu) of virus, 1-week-old suckling hamsters showed varying severity of neurological disease from 72hours post inoculation (hpi) and all of these animals had died by 96-áhpi. four-week-old icr mice inoculated orally with 4+ù10(4)pfu of virus showed no clinical signs, but they developed erosive and ulcerative gastritis from 36-áhpi. ... | 2011 | 21741053 |
| differences in kaposi sarcoma-associated herpesvirus-specific and herpesvirus-non-specific immune responses in classic kaposi sarcoma cases and matched controls in sicily. | kaposi sarcoma (ks) might develop because of incompetent immune responses, both non-specifically and specifically against the ks-associated herpesvirus (kshv). peripheral blood mononuclear cells from 15 classic (non-aids) ks cases, 13 kshv seropositives (without ks) and 15 kshv-seronegative controls were tested for interferon-+¦ t-cell (enzyme-linked immunospot [elispot]) responses to kshv-latency-associated nuclear antigen (lana), kshv-k8.1 and cmv/epstein-barr virus (ebv) peptide pools. the fo ... | 2011 | 21740480 |
| mammalian alphaherpesvirus mirnas. | mammalian alphaherpesviruses are major causes of human and veterinary disease. during productive infection, these viruses exhibit complex and robust patterns of gene expression. these viruses also form latent infections in neurons of sensory ganglia in which productive cycle gene expression is highly repressed. both modes of infection provide advantageous opportunities for regulation by micrornas. thus far, published data regarding micrornas are available for six mammalian alphaherpesviruses. no ... | 2011 | 21736960 |
| rodent models of pulmonary hypertension: harmonisation with the world health organisation's categorisation of human ph. | the who classification of pulmonary hypertension (ph) recognises five distinct groups, all sharing a mean, resting, pulmonary artery pressure (pap) > 25 mmhg. the aetiology of ph varies by group (1-pulmonary vascular disease, 2-high left heart filling pressures, 3-hypoxia, 4-unresolved pulmonary embolism and 5-miscellaneous). inclusion in a group reflects shared histological, haemodynamic and pathophysiological features and has therapeutic implications. advantages of using rodent models to under ... | 2011 | 21736677 |
| etiology of measles suspect cases reported in 2006-2007 in poland. | to achieve measles elimination, an efficient surveillance system for rash illnesses is necessary. the aim of the present study was to ascertain which viruses, other than measles, are causing measles-like illnesses (mlis) in poland. serum samples (n=278) collected from mli cases and submitted to the national reference laboratory during 2006-2007 were investigated for anti-measles (mev), rubella (rubv), parvovirus b19 (b19v), epstein-barr (ebv) and herpesvirus type-6 (hhv-6) igm presence. age was ... | 2011 | 21735834 |
| direct and specific binding of the ul16 tegument protein of herpes simplex virus to the cytoplasmic tail of glycoprotein e. | the ul16 tegument protein of herpes simplex virus (hsv) is conserved throughout all of the herpesvirus families. previous studies have shown that the binding of hsv to heparan sulfate molecules on the host cell triggers the release of ul16 from the capsid, but the mechanism by which the signal is sent from the virion surface into the tegument is unknown. here, we report that a glutathione s-transferase chimera bearing the cytoplasmic tail of viral glycoprotein e (ge) is capable of binding to ul1 ... | 2011 | 21734044 |
| ribosomal protein s6 interacts with the latency-associated nuclear antigen of kaposi's sarcoma-associated herpesvirus. | the latency-associated nuclear antigen (lana) is central to the maintenance of kaposi's sarcoma-associated herpesvirus (kshv) and to the survival of kshv-carrying tumor cells. in an effort to identify interaction partners of lana, we purified authentic high-molecular-weight complexes of lana by conventional chromatography followed by immunoprecipitation from the bc-3 cell line. this is the first analysis of lana-interacting partners that is not based on forced ectopic expression of lana. subsequ ... | 2011 | 21734034 |
| common, emerging, vector-borne and infrequent abortogenic virus infections of cattle. | this review deals with the aetiology and the diagnosis of bovine viral abortion. while the abortion rates on beef and dairy cattle farms usually do not exceed 10%, significant economic losses because of abortion storms may be encountered. determining the cause of abortions is usually a challenge, and it generally remains obscure in more than 50% of the necropsy submitted foetuses. bovine viral diarrhoea virus and bovine herpesvirus-1 are the most common viruses causally associated with bovine ab ... | 2011 | 21733134 |
| molecular detection of murine herpesvirus 68 in ticks feeding on free-living reptiles. | the mhv-68 (designed as murid herpesvirus 4 (muhv 4) strain 68) isolated from two rodents, myodes glareolus and apodemus flavicollis, is considered as a natural pathogen of free-living murid rodents. recently, the detection of mhv antibodies in the blood of animals living in the same biotope as mhv-infected mice has suggested that ticks may have a role in the transmission of this pathogen. ixodes ricinus is one the most abundant tick species in europe known to transmit multiple pathogens causing ... | 2011 | 21732020 |
| the p53 tumor suppressor is stabilized by inhibitor of growth 1 (ing1) by blocking polyubiquitination. | the inhibitor of growth tumor suppressors (ing1-ing5) affect aging, apoptosis, dna repair and tumorigenesis. plant homeodomains (phd) of ing proteins bind histones in a methylation-sensitive manner to regulate chromatin structure. ing1 and ing2 contain a polybasic region (pbr) adjacent to their phds that binds stress-inducible phosphatidylinositol monophosphate (ptin-mp) signaling lipids to activate these ings. ing1 induces apoptosis independently of p53 but other studies suggest proapoptotic in ... | 2011 | 21731648 |
| field efficacy of different vaccines against infectious bursal disease in broiler flocks. | a field study was performed to determine the efficacy of three commercially available vaccines against infectious bursal disease (ibd) in commercial broilers raised in a high ibd virus (ibdv) risk area. live attenuated intermediate and intermediate plus vaccines were used in four flocks. birds were vaccinated orally at the estimated vaccination time. three broiler flocks were vaccinated subcutaneously with a turkey herpesvirus (hvt)-ibd vector vaccine at one day old. evaluation of the efficacy o ... | 2011 | 21727070 |
| enzyme inhibition by allosteric capture of an inactive conformation. | all members of the human herpesvirus protease (hhv pr) family are active as weakly associating dimers but inactive as monomers. a small-molecule allosteric inhibitor of kaposi's sarcoma-associated herpesvirus protease (kshv pr) traps the enzyme in an inactive monomeric state where the c-terminal helices are unfolded and the hydrophobic dimer interface is exposed. nmr titration studies demonstrate that the inhibitor binds to kshv pr monomers with low micromolar affinity. a 2.0-+à-resolution x-ray ... | 2011 | 21723875 |
| nationwide cyprinid herpesvirus 3 contamination in natural rivers of japan. | cyprinid herpesvirus 3 (cyhv-3) disease is a significant threat for common and koi carp cultivators and for freshwater ecosystems. to determine the prevalence of cyhv-3 in japanese rivers, a nationwide survey of all national class-a rivers was undertaken in the summer of 2008. the virus was concentrated from river water samples using the cation-coated filter method. cyhv-3 dna was detected in 90 rivers, representing 90% of 103 successfully analysed rivers. more than 100,000 copies of cyhv-3 dna ... | 2011 | 21723573 |
| detection of a novel gammaherpesvirus in koalas (phascolarctos cinereus). | a novel gammaherpesvirus was detected in wild koalas (phascolarctos cinereus) captured at different locations during 2010. sequence analysis of the dna polymerase gene revealed that the virus was genetically distinct from all known gammaherpesviruses. this is the first herpesvirus to be definitively identified in the vombatiforme suborder (koalas and wombats). | 2011 | 21719855 |
| outbreak of human herpesvirus type 1 infection in nonhuman primates (callithrix penincillata). | human herpesvirus type 1 (hhv-1) is widely dispersed among the human population. although infection is often asymptomatic in humans, nonhuman primates develop a severe and often fatal infection. in august 2006, 13 black-tufted marmosets (callithrix penincillata) from a group of 14 presented with clinical apathy, anorexia, and ataxia. physical examination revealed conjunctivitis, erosive or ulcerative lesions on the skin, and swollen lymph nodes. of the 14 animals captured, 10 died. grossly, ulce ... | 2011 | 21719834 |
| assessment of clinical pathology and pathogen exposure in sea otters (enhydra lutris) bordering the threatened population in alaska. | northern sea otter (enhydra lutris kenyoni) abundance has decreased dramatically over portions of southwest alaska, usa, since the mid-1980s, and this stock is currently listed as threatened under the endangered species act. in contrast, adjacent populations in south central alaska, usa, and russia have been stable to increasing during the same period. sea otters bordering the area classified in the recent decline were live-captured during 2004-2006 at bering island, russia, and the kodiak archi ... | 2011 | 21719822 |
| epizootiologic investigations of selected abortive agents in free-ranging alpine ibex (capra ibex ibex) in switzerland. | in the early 2000s, several colonies of alpine ibex (capra ibex ibex) in switzerland ceased growing or began to decrease. reproductive problems due to infections with abortive agents might have negatively affected recruitment. we assessed the presence of selected agents of abortion in alpine ibex by serologic, molecular, and culture techniques and evaluated whether infection with these agents might have affected population densities. blood and fecal samples were collected from 651 ibex in 14 col ... | 2011 | 21719818 |
| inbreeding depression increases susceptibility to bovine tuberculosis in lions: an experimental test using an inbred-outbred contrast through translocation. | disease can dramatically influence the dynamics of endangered wildlife populations, especially when they are small and isolated, with increased risk of inbreeding. in hluhluwe-imfolozi park (hip), a small, enclosed reserve in south africa, a large lion (panthera leo) population arose from a small founder group in the 1960s and started showing conspicuous signs of inbreeding. to restore the health status of the hip lion population, outbred lions were translocated into the existing population. in ... | 2011 | 21719814 |
| the manipulation of mirna-gene regulatory networks by kshv induces endothelial cell motility. | mirnas have emerged as master regulators of cancer-related events. mirna dysregulation also occurs in kaposi sarcoma (ks). exploring the roles of ks-associated mirnas should help to identify novel angiogenesis and lymphangiogenesis pathways. in the present study, we show that kaposi sarcoma-associated herpesvirus (kshv), the etiological agent of ks, induces global mirna changes in lymphatic endothelial cells (lecs). specifically, the mir-221/mir-222 cluster is down-regulated, whereas mir-31 is u ... | 2011 | 21715310 |
| overexpression and purification of u24 from human herpesvirus type-6 in e. coli: unconventional use of oxidizing environments with a maltose binding protein-hexahistine dual tag to enhance membrane protein yield. | abstract: | 2011 | 21714924 |
| human herpesvirus 8 seropositivity among sexually active adults in uganda. | sexual transmission of human herpesvirus 8 (hhv8) has been implicated among homosexual men, but the evidence for sexual transmission among heterosexual individuals is controversial. we investigated the role of sexual transmission of hhv8 in a nationally representative sample in uganda, where hhv8 infection is endemic and transmitted mostly during childhood. | 2011 | 21712983 |
| development of a sybr green quantitative polymerase chain reaction assay for rapid detection and quantification of infectious laryngotracheitis virus. | infectious laryngotracheitis is an acute viral respiratory disease of chickens with a worldwide distribution. sensitive detection of the causative herpesvirus is particularly important because it can persist in the host at a very low copy number and be transmitted to other birds. quantification of viral genome copy number is also useful for clinical investigations and experimental studies. in the study presented here, a quantitative polymerase chain reaction (qpcr) assay was developed using sybr ... | 2011 | 21711182 |
| constitutive nf-{kappa}b activation confers interleukin 6 (il6) independence and resistance to dexamethasone and janus kinase inhibitor incb018424 in murine plasmacytoma cells. | myeloma cells are dependent on il6 for their survival and proliferation during the early stages of disease, and independence from il6 is associated with disease progression. the role of the nf-+¦b pathway in the il6-independent growth of myeloma cells has not been studied. because human herpesvirus 8-encoded k13 selectively activates the nf-+¦b pathway, we have used it as a molecular tool to examine the ability of the nf-+¦b pathway to confer il6 independence on murine plasmacytomas. we demonstr ... | 2011 | 21705340 |
| human herpesvirus 8 is not detectable in lesions of large plaque parapsoriasis, and in early-stage sporadic, familial, and juvenile cases of mycosis fungoides. | background: human herpesvirus (hhv) 8, an essential etiologic agent of kaposi sarcoma, is also associated with several lymphoproliferative disorders. the involvement of hhv 8 in mycosis fungoides (mf) and large plaque parapsoriasis (lpp) is controversial, with contradictory reports from various countries worldwide. objective: we sought to investigate the presence of the hhv 8 genome in skin lesions of lpp and early-stage sporadic, familial, and juvenile mf in patients in israel. methods: archiva ... | 2011 | 21704421 |
| aspects of bovine herpesvirus-1 infection in dairy and beef herds in the republic of ireland. | infection with bovine herpesvirus-1 (bhv-1) causes a wide range of disease manifestations, including respiratory disease and abortion, with world-wide distribution. the primary objective of the present study was to describe aspects of bhv-1 infection and control on irish farms, including herd-level seroprevalence (based on pooled sera) and vaccine usage. | 2011 | 21699677 |
| the intrinsic antiviral defense to incoming hsv-1 genomes includes specific dna repair proteins and is counteracted by the viral protein icp0. | cellular restriction factors responding to herpesvirus infection include the nd10 components pml, sp100 and hdaxx. during the initial stages of hsv-1 infection, novel sub-nuclear structures containing these nd10 proteins form in association with incoming viral genomes. we report that several cellular dna damage response proteins also relocate to sites associated with incoming viral genomes where they contribute to the cellular front line defense. we show that recruitment of dna repair proteins t ... | 2011 | 21698222 |
| kaposi's sarcoma-associated herpesvirus-encoded latency-associated nuclear antigen reduces interleukin-8 expression in endothelial cells and impairs neutrophil chemotaxis by degrading nuclear p65. | latency-associated nuclear antigen 1 (lana-1) of kaposi's sarcoma-associated herpesvirus (kshv) is the major viral latent protein and functions as a multifaceted protein. here, we report that lana-1 attenuates the endothelial response to tumor necrosis factor alpha (tnf-+¦) stimulation and inhibits consequent neutrophil chemotaxis. reporter assays showed that lana-1 constantly repressed nuclear factor (nf)-+¦b transactivity upon tnf-+¦ stimulation. we also found that lana-1 decreased nuclear p65 ... | 2011 | 21697472 |
| kshv virf-2 inhibits type 1 interferon signalling by targeting isgf-3. | kaposi's sarcoma-associated herpesvirus (kshv) encodes four viral interferon regulatory factors (virf-1-4). we investigated the mechanism and consequences of virf-2-mediated inhibition of interferon-response element (isre) signalling following type i interferon (ifn) induction. western blot and electrophoretic mobility shift assays identified the interferon stimulated gene factor-3 (isgf-3) components stat-1 and irf-9 as the proximal targets of virf-2 activity. the biological significance of vir ... | 2011 | 21697347 |
| retroviral expression of mir2 decreases both surface mhc class i and the alloimmune ctl response. | the immune response to allogeneic cells in tissue-engineered constructs is a major barrier to their successful application in the treatment of many human diseases. specifically, the t cell-mediated immune response, initiated through the recognition of cell surface mhci molecules, is the primary cause of acute cellular allograft rejection. in this study, we altered expression of mhci through viral immunomodulatory mechanisms to examine whether allogeneic cells could be made to 'mimic' viral evasi ... | 2011 | 21695793 |
| establishment of a novel ovine kidney cell line for isolation and propagation of viruses infecting domestic cloven-hoofed animal species. | a sheep kidney-derived cell line, flk-n3, was successfully established after serial (>100) passages. persistent infection of this cell line with viruses and mycoplasma was not detected. the cells grew well and showed susceptibility to a wide variety of viruses derived from ovine, bovine, and porcine species, including orf virus, maedi visna virus, bovine herpesvirus 1, bovine parainfluenza virus 3, bovine viral diarrhea viruses 1 and 2, bovine coronavirus, bovine respiratory syncytial virus, bov ... | 2011 | 21695582 |
| [viral infection risk in polytransfused adults: seroprevalence of seven viruses in central tunisia]. | the aim of this study is to evaluate the prevalence of seven transfusion-transmitted viruses in polytransfused adults and children comparatively with a group of healthy control subjects. we studied 107 polytransfused patients (59 adults and 48 children) and 160 control subjects (100 blood donors and 60 children). immunoenzymatic tests were used for detection of hbs antigen (hbs ag), antibodies against hepatitis c virus (anti-hcv), and human immunodeficiency virus (anti-hiv), and igg antibodies a ... | 2011 | 21695497 |
| a-ásilence element involved in kaposi+äs-ásarcoma-associated herpesvirus orf11 gene expression. | 2011 | 21692569 | |
| comparison of elisa and virus neutralization test in assaying serum antibodies to bovine herpesvirus 1. | 2011 | 21692568 | |
| increased prevalence of kaposi+äs sarcoma-associated herpesvirus in the kaposi+äs sarcoma-endemic area of western kenya in 1981-2000. | kaposi+äs sarcoma (ks) had been endemic in africa before the appearance of human immunodeficiency viruses (hiv) in 1985. incidence of african ks has increased over the time and the risk of contracting ks become greater in hiv-positive as opposed to hiv-negative individuals. ks specimens were collected in 1981-2000 from 228 surgical cases originating from a ks-endemic area of western kenya and examined for kaposi+äs sarcoma-associated herpesvirus (kshv) by an immunoperoxidase assay. the results s ... | 2011 | 21692565 |
| expanding the role of drosha to the regulation of viral gene expression. | it is well-appreciated that viruses use host effectors for macromolecular synthesis and as regulators of viral gene expression. viruses can encode their own regulators, but often use host-encoded factors to optimize replication. here, we show that drosha, an endoribonuclease best known for its role in the biogenesis of micrornas (mirnas), can also function to directly regulate viral gene expression. kaposi's sarcoma-associated herpesvirus (kshv) is associated with various tumors, and like all he ... | 2011 | 21690333 |
| equid herpesvirus type-1 exhibits neurotropism and neurovirulence in a mouse model. | intranasal inoculation of equid herpesvirus type-1 (ehv-1) brazilian strains a4/72 and a9/92 induced an acute and lethal infection in four different inbred mouse strains. clinical and neurological signs appeared between the 2nd and 3rd day post inoculation (dpi) and included weight loss, ruffled fur, a hunched posture, crouching in corners, nasal and ocular discharges, dyspnoea, dehydration and increased salivation. these signs were followed by increased reactivity to external stimulation, seizu ... | 2011 | 21689826 |
| cytoplasmic localized infected cell protein 0 (bicp0) encoded by bovine herpesvirus 1 inhibits +¦ interferon promoter activity and reduces irf3 (interferon response factor 3) protein levels. | bovine herpesvirus 1 (bhv-1), an alpha-herpesvirinae subfamily member, establishes a life-long latent infection in sensory neurons. periodically, bhv-1 reactivates from latency, infectious virus is spread, and consequently virus transmission occurs. bhv-1 acute infection causes upper respiratory track infections and conjunctivitis in infected cattle. as a result of transient immune-suppression, bhv-1 infections can also lead to life-threatening secondary bacterial pneumonia that is referred to a ... | 2011 | 21689696 |
| the polymorphic hcmv glycoprotein ul20 is targeted for lysosomal degradation by multiple cytoplasmic dileucine motifs. | human cytomegalovirus (hcmv) is a widespread and persistent beta-herpesvirus. the large dna genome of hcmv encodes many proteins that are non-essential for viral replication including numerous proteins subverting host immunosurveillance. one of them is the barely characterized ul20, which is encoded adjacent to the well-defined immunoevasins ul16 and ul18. ul20 is a type i transmembrane glycoprotein with an immunoglobulin-like ectodomain that is highly polymorphic among hcmv strains. here, we sh ... | 2011 | 21689255 |
| human herpesvirus type 6 hepatitis or familiar intrahepatic cholestasis: the importance of follow-up. | a 1-month-old child presented to our unit with jaundice and raised aminotransferases, +¦-glutamyltranspeptidase and bilirubin. metabolic diseases were ruled out and ultrasound found no alterations. human herpesvirus type 6 (hhv-6) dna was found in blood and saliva and igg anti-hhv-6 in serum, and a diagnosis of hhv-6 hepatitis was made. in the following weeks, aminotransferase values remained raised while +¦-glutamyltranspeptidase levels returned to normal in 45 days. at the age of 5 months symp ... | 2009 | 21686719 |
| tumor suppressor genes fhit and wwox are deleted in primary effusion lymphoma (pel) cell lines. | primary effusion lymphoma (pel) is a diffuse-large b-cell lymphoma with poor prognosis. one hundred percent of pels carry the genome of kaposi sarcoma-associated herpesvirus and a majority are coinfected with epstein-barr virus (ebv). we profiled genomic aberrations in pel cells using the affymetrix 6.0 snp array. this identified for the first time individual genes that are altered in pel cells. eleven of 13 samples (85%) were deleted for the fragile site tumor suppressors wwox and fhit. alterat ... | 2011 | 21685375 |
| molecular data of ul24 homolog gene (orf37) from brazilian isolates of equine herpesvirus type 1. | equine herpesvirus type 1 (ehv-1) is associated with abortions, respiratory distress, and neurological disturbances in horses. the orf37 of ehv-1 encodes a protein homolog to ul24 gene product of human herpesvirus that has been associated with neurovirulence. in the present work, orf37 pcr fragments derived from two brazilian ehv-1 isolates, a german isolate and an american reference strain were sequenced and characterized by molecular phylogenetic analysis. this genomic region is highly conserv ... | 2011 | 21684566 |
| roles of avian herpesvirus micrornas in infection, latency, and oncogenesis. | micrornas have been reported for the avian herpesviruses marek's disease virus 1 (mdv1; oncogenic), marek's disease virus 2 (mdv2; non-oncogenic), herpesvirus of turkeys (hvt), and infectious laryngotracheitis virus (iltv). no obvious phylogenetic relationships exist among the avian herpesvirus micrornas, but the general genomic locations of microrna clusters are conserved, with micrornas being located in the repeat regions of the genomes. in some cases, micrornas are antisense to open reading f ... | 2011 | 21683170 |
| nuclear envelope breakdown can substitute for primary envelopment-mediated nuclear egress of herpesviruses. | herpesvirus nucleocapsids assemble in the nucleus but mature to infectious virions in the cytoplasm. to gain access to this cellular compartment, nucleocapsids are translocated to the cytoplasm by primary envelopment at the inner nuclear membrane and subsequent fusion of the primary envelope with the outer nuclear membrane. the conserved viral pul34 and pul31 proteins play a crucial role in this process. in their absence, viral replication is strongly impaired but not totally abolished. we used ... | 2011 | 21680518 |
| secondary lymphoid tissue as an important site for wu polyomavirus infection in immunocompetent children. | the polyomaviruses ki and wu (kipyv and wupyv) have been identified in respiratory specimens from children with acute respiratory infections, which suggests the respiratory tract as a possible site of infection. however, the persistence of infection in the lymphoid system is unknown. fresh samples (n = 211) of tonsils, adenoids, and peripheral blood mononuclear cells (pbmcs) from 83 immunocompetent children (mean age 4.8 years) were tested for amplification of the kipyv vp1 and wupyv vp2 genes. ... | 2011 | 21678449 |
| positive epstein-barr virus and cytomegalovirus igm assays in primary hiv infection. | we report three cases with misleading cytomegalovirus (cmv) or epstein-barr virus (ebv) immunoglobulin m (igm) results during primary human immunodeficiency virus (hiv) infection. we determined the rate of positive anti-cmv igm assays or anti-ebv capsid antigen igm assays in sera from a group of well-characterized subjects with primary hiv infection as 2.9% (1/35; 95%ci: 0.15-16.6%) for each infection. the rate of positive anti-ebv capsid antigen igm assays in subjects with positive hepatitis a ... | 2011 | 21678445 |