Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [indication, genotyping and phylogenetic analysis of bovine diarrhea virus obtained in the territory of the russian federation ]. | bovine diarrhea virus (bdv) is widespread throughout the european part of the russian federation. the bdv genome is detected in around 40% of animals (aged up to 18 months) with lesions in the gastrointestinal tract and respiratory system. it can be found in all intestinal sections, including its contents and feces, in lymphoid and respiratory organs as well as in nasal washings and discharges and in vaginal tunic discharges of cows. primary structure analysis of 5'-untransalted region of the ge ... | 2003 | 14598481 |
| borna disease virus phosphoprotein represses p53-mediated transcriptional activity by interference with hmgb1. | borna disease virus (bdv) is a noncytolytic, neurotropic rna virus that has a broad host range in warm-blooded animals, probably including humans. recently, it was demonstrated that a 24-kda phosphoprotein (p) of bdv directly binds to a multifunctional protein, amphoterin-hmgb1, and inhibits its function in cultured neural cells (w. kamitani, y. shoya, t. kobayashi, m. watanabe, b. j. lee, g. zhang, k. tomonaga, and k. ikuta, j. virol. 75:8742-8751, 2001). this observation suggested that express ... | 2003 | 14581561 |
| borna disease virus glycoprotein is required for viral dissemination in neurons. | borna disease virus (bdv) is a nonsegmented negative-strand rna virus with a tropism for neurons. infection with bdv causes neurological diseases in a wide variety of animal species. although it is known that the virus spreads from neuron to neuron, assembled viral particles have never been visualized in the brains of infected animals. this has led to the hypothesis that bdv spreads as nonenveloped ribonucleoproteins (rnp) rather than as enveloped viral particles. we assessed whether the viral e ... | 2003 | 14581559 |
| a reverse genetics system for borna disease virus. | borna disease virus (bdv) is an enveloped virus. its non-segmented, negative-stranded rna genome has the coding capability for six main polypeptides and has an organization characteristic of members of the order mononegavirales. however, based on its unique genetics and biological features, bdv is considered to be the prototypic member of a new virus family, bornaviridae. here, the establishment of a reverse genetics system for bdv is described. intracellular synthesis of a bdv rna analogue or m ... | 2003 | 14573815 |
| infectious bursal disease virus and proventriculitis in broiler chickens. | acute necrotic proventriculitis is a naturally occurring disease of broiler chickens that causes proventricular rupture during routine evisceration. although infectious bursal disease virus (ibdv) has been implicated, it has not been proven to be a direct cause of this disease. to further study the role of ibdv in proventriculitis, proventriculi and bursas were collected during both acute and chronic phases of naturally occurring proventriculitis and from chickens experimentally infected with se ... | 2003 | 14562897 |
| active borna disease virus polymerase complex requires a distinct nucleoprotein-to-phosphoprotein ratio but no viral x protein. | analysis of the composition and regulation of the borna disease virus (bdv) polymerase complex has so far been limited by the lack of a functional assay. to establish such an assay on the basis of an artificial minigenome, we constructed expression vectors encoding either nucleoprotein (n), phosphoprotein (p), x protein, or polymerase (l) of bdv under the control of the chicken beta-actin promoter. a flag-tagged version of l colocalized with virus-encoded n and p in characteristic nuclear dots o ... | 2003 | 14557662 |
| [the mechanism of persistent infection and pathogenesis of borna disease virus]. | 2003 | 14556510 | |
| detection of anti-borna disease virus (bdv) antibodies from patients with schizophrenia and mood disorders in japan. | the relationship between infection with the borna disease virus (bdv) and the clinical symptoms of schizophrenia and mood disorders (dms-iv) was investigated. western blotting techniques were used to examine anti-p10-bdv antibodies in serum from 32 patients with schizophrenia and 33 patients with mood disorders in japan. the results showed that 1 out of 25 controls (4.0%), 7 out of 32 patients with schizophrenia (21.9%) and 9 out of 33 patients with mood disorders (27.3%) were positive for anti- ... | 2003 | 14527651 |
| a system of protein target sequences for anti-rna-viral chemotherapy by a vitamin b6-derived zinc-chelating trioxa-adamantane-triol. | the synthesis of the structurally unusual heterotricyclic compound 1-[3-hydroxy-5-(hydroxymethyl)-2-methyl-4-pyridinyl]-2,8,9-trioxaadamantane-3,5,7-triol (trivially named bananin, bn) from pyridoxylidenephloroglucinol and a theoretical prospect on possible biological activities of bn are presented in this report. pyridoxylidenephloroglucinol is synthesized by knoevenagel condensation of the vitamin b6 aldehyde pyridoxal with phloroglucinol. pyridoxylidenephloroglucinol rearranges to light-yello ... | 2003 | 14527557 |
| crystal structure of the borna disease virus nucleoprotein. | borna disease virus (bdv) causes an infection of the central nervous system in a wide range of vertebrates, which can fatally progress to an immune-mediated disease, called borna disease. bdv is a member of the mononegavirales, which also includes the highly infectious measles and ebola viruses. the viral nucleoproteins are central to transcription, replication, and packaging of the rna genome. we present the x-ray structure of the bdv nucleoprotein determined at 1.76 a resolution. the structure ... | 2003 | 14527390 |
| unraveling the replication machine from negative-stranded rna viruses. | 2003 | 14527386 | |
| borna disease virus nucleoprotein interacts with the cdc2-cyclin b1 complex. | transition from g(2) to m phase, a cell cycle checkpoint, is regulated by the cdc2-cyclin b1 complex. here, we report that persistent infection with borna disease virus (bdv), a noncytolytic rna virus infecting the central nervous system, results in decelerated proliferation of infected host cells due to a delayed g(2)-to-m transition. persistent bdv-infected rat fibroblast cells showed reduced proliferation compared to uninfected cells. in pull-down assays we observed an interaction of the vira ... | 2003 | 14512566 |
| [cerebrospinal fluid filtration as experimental therapy in therapy refractory psychoses in borna disease virus seropositive patients. therapeutic effects, findings]. | according to previous investigations, mild borna disease virus encephalitis may underlie a subgroup of affective or schizophrenic type psychoses. and virus-induced immune pathology may underlie even a larger subgroup of psychoses. we treated bdv seropositive patients suffering from therapy resistant schizophrenic or affective spectrum psychoses by cerebrospinal fluid filtration (csff) in an experimental add-on treatment. csff was shown previously to be an effective immune modulatory treatment in ... | 2003 | 14509081 |
| [csf filtration as experimental therapy in therapyresistant psychoses in borna disease virus-seropositive patients] | according to previous investigations, mild borna disease virus encephalitis may underlie a subgroup of affective or schizophrenic type psychoses. and virus-induced immune pathology may underlie even a larger subgroup of psychoses. we treated bdv seropositive patients suffering from therapy resistant schizophrenic or affective spectrum psychoses by cerebrospinal fluid filtration (csff) in an experimental add-on treatment. csff was shown previously to be an effective immune modulatory treatment in ... | 2003 | 13130379 |
| trafficking of viral genomic rna into and out of the nucleus: influenza, thogoto and borna disease viruses. | most rna viruses that lack a dna phase replicate in the cytoplasm. however, several negative-stranded rna viruses such as influenza, thogoto, and borna disease viruses replicate their rnas in the nucleus, taking advantage of the host cell's nuclear machinery. a challenge faced by these viruses is the trafficking of viral components into and out of the nucleus through the nuclear membrane. the genomic rnas of these viruses associate with proteins to form large complexes called viral ribonucleopro ... | 2003 | 12921991 |
| experimental vertical transmission of borna disease virus in the mouse. | we demonstrated the experimental vertical transmission of borna disease virus (bdv) in pregnant balb/c mice. giessen strain he/80 of bdv was used in the present study. six six-week-old mice were inoculated intraperitoneally with 10(5) 50% tissue culture infective doses (tcid50), and were bred immediately. four pregnant mice were sacrificed under anaesthesia on the 10th and 14th days after vaginal plug formation. nine newborns from two maternal mice were sacrificed under anaesthesia on the 7th da ... | 2003 | 12898331 |
| exploring the cerebellum with a new tool: neonatal borna disease virus (bdv) infection of the rat's brain. | cerebellar pathology has been associated with a number of developmental behavioral disorders, including autism spectrum disorders. despite the fact that perinatal virus infections have been implicated in neurodevelopmental damage, few animal models have been developed to study the pathogenesis involved. one of the most interesting in vivo models of virus-induced cerebellar damage is the neonatal borna disease virus (bdv) infection of the rat brain. the present review describes molecular, cellula ... | 2003 | 12882236 |
| [detection of borna disease virus-p24 specific antibody in the sera of schizophrenic patients of china by means of western-blot]. | to investigate whether borna disease virus (bdv) infection is related to the schizophrenic patients from china. | 2003 | 12870028 |
| glial expression of borna disease virus phosphoprotein induces behavioral and neurological abnormalities in transgenic mice. | one hypothesis for the etiology of behavioral disorders is that infection by a virus induces neuronal cell dysfunctions resulting in a wide range of behavioral abnormalities. however, a direct linkage between viral infections and neurobehavioral disturbances associated with human psychiatric disorders has not been identified. here, we show that transgenic mice expressing the phosphoprotein (p) of borna disease virus (bdv) in glial cells develop behavioral abnormalities, such as enhanced intermal ... | 2003 | 12857949 |
| borna disease virus infection, a human mental-health risk. | this article focuses on human borna disease virus (bdv) infections, most notably on the development of valid diagnostic systems, which have arisen as a major research issue in the past decade. the significance of a novel modular triple enzyme-linked immunosorbent assay that is capable of specifically measuring anti-bdv antibodies as well as major structural proteins n (p40) and p (p24) in the blood, either as free antigens in the plasma or as antibody-bound circulating immune complexes (cics), i ... | 2003 | 12857781 |
| genetic and antigenic characterization of novel pestivirus genotypes: implications for classification. | currently, the genus pestivirus comprises the four approved species bovine viral diarrhea virus 1 (bvdv-1), bvdv-2, classical swine fever virus (csfv), and border disease virus (bdv) and one tentative fifth species represented by a single strain (h138) isolated from a giraffe in kenya more than 30 years ago. to further address the issue of heterogeneity of pestiviruses we have determined the entire n(pro) and e2 coding sequences for several new pestivirus isolates. interestingly, phylogenetic an ... | 2003 | 12832207 |
| modulation of borna disease virus phosphoprotein nuclear localization by the viral protein x encoded in the overlapping open reading frame. | borna disease virus (bdv) is a nonsegmented, negative-strand rna virus that belongs to the mononegavirales order. unlike other animal viruses in this order, bdv replicates and transcribes in the nucleus of infected cells. therefore, regulation of the intracellular movement of virus components must be critical for accomplishing the bdv life cycle in mammalian cells. previous studies have demonstrated that bdv proteins are prone to accumulate in the nucleus of cells transiently transfected with ea ... | 2003 | 12829848 |
| hypothalamic-pituitary-adrenal (hpa) system activity in depression and infection with borna disease virus and chlamydia pneumoniae. | 2003 | 12808426 | |
| [heterogeneity of ruminant pestiviruses: academic interest or important basis for the development of vaccines and diagnostics?]. | pestiviruses cause economically important diseases of farm animals. members of the pestiviruses are bovine viral diarrhea virus 1 (bvdv-1), bvdv-2, classical swine fever virus (csfv) and border disease virus (bdv). phylogenetic analyses based on the entire nucleic acid sequence encoding the npro allow a statistically significant segregation of established species and of subgroups within the species. bvdv-1 strains isolated in germany can be associated with at least five different subgroups. in c ... | 2003 | 12784555 |
| borna disease virus induces acute fatal neurological disorders in neonatal gerbils without virus- and immune-mediated cell destructions. | borna disease virus (bdv) is a noncytolytic, neurotropic rna virus that is known to cause neurological disturbances in various animal species. our previous experiment demonstrated that neonate gerbils develop an acute fatal neurological disease following infection with bdv, virology 282, 65-76). the study suggested that bdv directly causes functional damage of neuronal cells resulting in the lethal disorder in neonatal gerbils. to extend this finding, we examined whether bdv can induce neurologi ... | 2003 | 12781712 |
| precursors of borna disease virus-specific t cells in secondary lymphatic tissue of experimentally infected rats. | borna disease in rats represents an experimental model to study the immunopathological role of t cells in central nervous system disease. adoptive transfer experiments were performed to investigate homing properties of t cells that infiltrate the brains of infected animals. lymphocytes isolated from the brains of diseased rats were labelled with 5,6-carboxyfluorescein diacetate succinimidyl ester (cfse) and transferred into immunosuppressed infected recipients. in recipient rats displaying neuro ... | 2003 | 12775416 |
| [borna disease virus infection]. | 2003 | 12722201 | |
| two major histocompatibility complex class i-restricted epitopes of the borna disease virus p10 protein identified by cytotoxic t lymphocytes induced by dna-based immunization. | borna disease virus (bdv) infection of lewis rats is the most studied animal model of borna disease, an often fatal encephalomyelitis. in this experimental model, bdv-specific cd8(+) cytotoxic t lymphocytes (ctls) play a prominent role in the immunopathogenesis of infection by the noncytolytic, persistent bdv. of the six open reading frames of bdv, ctls to bdv x (p10) and the l-polymerase have never been studied. in this study, we used plasmid immunization to investigate the ctl response to bdv ... | 2003 | 12719601 |
| borna disease virus. | borna disease virus, a negative-strand rna virus, infects a wide variety of warm-blooded animals. depending on the age of the host and the integrity of its immune response, infection may be asymptomatic or cause a broad spectrum of behavioral disorders. unusual features of borna disease virus biology include nuclear localization of replication and transcription; diverse strategies for regulation of gene expression; and interaction with signaling pathways resulting in subtle neuropathology. altho ... | 2003 | 12707857 |
| the envelope glycoprotein e2 is a determinant of cell culture tropism in ruminant pestiviruses. | bovine viral diarrhoea virus (bvdv) isolates infect cultured madin-darby bovine kidney (mdbk) cells as efficiently as sheep kidney cells. in contrast, border disease virus (bdv) propagates poorly in mdbk cells but infects sheep cells very efficiently. the envelope glycoprotein e2 has been shown to be essential for virus infectivity. to explore the potential role of e2 in pestivirus host range in cell cultures, we engineered a chimeric bvdv with the e2 coding region from bdv. as expected, the bvd ... | 2003 | 12692293 |
| genetic relationship of borna disease virus isolates. | the infection of humans with boma disease virus (bdv) is still a matter of debate. in a recent publication, we described a bdv (rw98) isolated from the blood of a psychiatric patient. the rna of this virus differed more than 5% from that of the widely used strain he/80, which was supposed to represent our laboratory virus. here, we show that the virus used in our laboratory was not he/80 and, furthermore, that rw98 has sequence identity to the laboratory strain. we also present data that bdv-spe ... | 2003 | 12680690 |
| neuropharmacological sequelae of persistent cns viral infections: lessons from borna disease virus. | borna disease virus (bdv) is a neurotropic rna virus that is worldwide in distribution, causing movement and behavior disorders in a wide range of animal species. bdv has also been reported to be associated with neuropsychiatric diseases of humans by serologic study and by recovery of nucleic acid or virus from blood or brain. natural infections of horses and sheep produce encephalitis with erratic excited behaviors, hyperkinetic movement or gait abnormalities; naturally infected cats have ataxi ... | 2003 | 12667891 |
| characterization of the acute immune response in the retina of borna disease virus infected lewis rats. | in lewis rats infected intracerebrally with the highly neurotropic borna disease virus (bdv), the retina is one of the most severely affected central nervous system (cns) structures. while bdv-induced damage in the brain has been previously shown to be caused by a t-cell-dependent process, the immunopathological mechanisms leading to bdv-induced retinitis remain to be elucidated. rna samples from retinae were subjected to rnase protection assays to detect transcripts of proinflammatory cytokines ... | 2003 | 12667649 |
| selective virus resistance conferred by expression of borna disease virus nucleocapsid components. | persistent viral infections can render host cells resistant to superinfection with closely related viruses by largely uncharacterized mechanisms. we present evidence for superinfection exclusion in brains of borna disease virus (bdv)-infected rats and in persistently infected vero cells, and we suggest that acquired resistance to bdv is due to unbalanced intracellular levels of viral nucleocapsid components. we observed that expression of bdv protein p, n, or x rendered human cells resistant to ... | 2003 | 12634385 |
| accumulation of the proinflammatory cytokine endothelial-monocyte-activating polypeptide ii in ramified microglial cells in brains of borna virus infected lewis rats. | borna disease virus (bdv) infection of adult lewis rats induces a severe and often fatal neurologic disease characterized by a massive mononuclear meningo-encephalitis, and activation of microglial cells. therefore, we analyzed expression of endothelial monocyte activating polypeptide ii (emap ii) by immunohistology as a marker for activation of microglial cells in bdv infected rat brains. emap ii is a chemotactic peptide, inducing activation of macrophages and endothelial cells, and is consider ... | 2003 | 12633891 |
| genetic diversity of ruminant pestiviruses from spain. | the genetic diversity of ruminant pestiviruses from spain was investigated by sequencing analysis of the 5' non-coding region (5'ncr) from 10 ovine and 41 bovine samples collected along 4 years (1999-2002) from different farms. the 5'ncr amplicons generated by a one-tube rt-pcr using primers 324/326 were sequenced and phylogenetically analyzed. when compared with strains from genbank database, spanish viruses clustered into three genotypes: bvdv 1, bdv and csfv. no bvdv 2 strains were identified ... | 2003 | 12606077 |
| [kinetics and persistence of neutralizing antibodies against bovine viral diarrhea virus-1 and -2 and border disease virus after two step vaccination of cattle]. | the aim of the study was the assessment of rise and persistence of neutralizing antibodies (nab) to bovine viral diarrhea virus (bvdv) and border disease virus (bdv) after a two step vaccination using an inactivated bvdv/bdv (mucobovin) and a modified live bvdv vaccine (vacoviron). in a first experiment eight heifers were kept in isolation and were serologically surveyed regularly over a three year period after vaccination. the same experiment was done with 80 vaccinated cattle kept under field ... | 2003 | 12596665 |
| identification of differentially expressed genes in brains of newborn borna disease virus-infected rats in the absence of inflammation. | infection of newborn rats with borna disease virus (bdv) leads to viral persistence in the central nervous system without overt signs of inflammation. nevertheless, these rats display distinct behavioral and neurodevelopmental abnormalities. the molecular basis of the latter is still unknown. using a cdna array representing 1200 genes, we sought to identify cellular genes which are differentially expressed following perinatal bdv-infection. rna samples prepared from different brain regions were ... | 2003 | 12536295 |
| seroprevalence of borna disease virus antibodies is not increased in patients with amyotrophic lateral sclerosis. | 2002 | 12532937 | |
| higher prevalence of anti-borna disease virus antibodies in stabled than in feral horses in japan. | 2002 | 12455848 | |
| key role for enkephalinergic tone in cortico-striatal-thalamic function. | whereas the role of dopaminergic tone in the cortico-striatal-thalamic system is well-established, the role of endogenous opioids in the function of this system is less understood. we show that borna disease virus infection of adult rats results in an increase in preproenkephalin transcripts in the striatum of borna-infected rats, a region important for forming coordinated sequential motor actions and in developing programmes of thought and motivation. stereotypic behaviours and dyskinesias, the ... | 2002 | 12431235 |
| bornavirus and the brain. | borna disease virus (bdv) causes central nervous system (cns) disease that is frequently manifested by behavioral abnormalities. bdv is a nonsegmented, negative, single-stranded rna virus. on the basis of its unique genetic and biologic features, bdv is the prototypic member of a new virus family, bornaviridae, within the order mononegavirales. therefore, the investigation of the molecular and cell biology of bdv may provide new insights about virus-cell interactions in the cns. bdv is an import ... | 2002 | 12424704 |
| identification of the amino terminal subunit of the glycoprotein of borna disease virus. | the only surface membrane glycoprotein of borna disease virus (bdv) is synthesized as a polypeptide with a molecular mass of 57 kda and n-glycosylated to a precursor glycoprotein (gp) of about 94 kda. it is processed by the cellular protease furin into the c-terminal membrane-anchored subunit gp-c, also known as gp43, and a presumptive n-terminal subunit gp-n, that is highly glycosylated and has a molecular mass of about 51 kda. however, up to now the latter remained undetected in bdv-infected m ... | 2002 | 12417322 |
| borna disease virus accelerates inflammation and disease associated with transgenic expression of interleukin-12 in the central nervous system. | targeted expression of biologically active interleukin-12 (il-12) in astrocytes of the central nervous system (cns) results in spontaneous neuroimmunological disease of aged mice. borna disease virus (bdv) can readily multiply in the mouse cns but does not trigger disease in most strains. here we show that a large percentage of il-12 transgenic mice developed severe ataxia within 5 to 10 weeks after infection with bdv. by contrast, no disease developed in mock-infected il-12 transgenic and wild- ... | 2002 | 12414961 |
| humoral immune response against borna disease virus (bdv) in experimentally and naturally infected cats. | in order to investigate the peripheral and intracerebral humoral immune response against borna disease virus (bdv) in cats, serum and cerebrospinal fluid (csf) samples from experimentally and naturally bdv-infected cats were analysed in two different test systems (indirect enzyme-linked immunosorbent assay and indirect immunofluorescent test). the experimentally infected cats developed high antibody titres against the major immunogenic bdv-proteins, p24 and p40. in contrast, the naturally infect ... | 2002 | 12406652 |
| [borna disease virus: its broad host range and neuropathogenesis]. | 2002 | 12227178 | |
| borna disease virus and psychiatric disorders. | 2002 | 12223262 | |
| madness. | 2002 | 12219740 | |
| borna disease virus--reactive antibodies in polish psychiatric patients. | it has been proposed that the borna disease virus (bdv) plays a role in the etiopathogenesis of psychiatric disorders. we assessed bdv seropositivity in polish psychiatric patients and healthy controls, and the relationship between seropositivity and selected diagnostic and clinical variables. | 2002 | 12218946 |
| persistence of borna disease virus in naturally infected sheep. | to get an insight into borna disease virus (bdv) epidemiology, an isolated flock of approximately 25 sheep within the region of southeast germany to which the disease is endemic was investigated over a 3-year observation period. bdv-specific antibodies and rna in peripheral blood mononuclear cells were detected in 12.5 (year 1), 11.5 (year 2), and 19.4% (year 3) and 1.6 (year 1), 0 (year 2), and 14.9% (year 3) of the animals, respectively. bdv persisted in asymptomatic sheep for up to 2 years. s ... | 2002 | 12208952 |
| changes of the organotypic retinal organization in borna virus-infected lewis rats. | retinae of borna disease virus (bdv)-infected lewis rats were investigated with emphasis on long-term changes in organotypic tissue organization and glia-neuron relationship. virus inoculation was attained via intracerebral bdv injection. following survival times ranging between two and eight months, the retinal thickness was reduced up to one third of that of controls. photoreceptor segments were completely extinguished and the number of neurons was dramatically reduced. the typical laminar org ... | 2001 | 12165671 |
| enhanced neurovirulence of borna disease virus variants associated with nucleotide changes in the glycoprotein and l polymerase genes. | borna disease virus (bdv) infection produces a variety of clinical diseases, from behavioral illnesses to classical fatal encephalitis (i.e., borna disease [bd]). since the genomes of most bdv isolates differ by less than 5%, host factors are believed responsible for much of the reported variability in disease expression. the contribution of bdv genomic differences to variation in bd expression is largely unexplored. here we compared the clinical outcomes of rats infected with one of two related ... | 2002 | 12163584 |
| borna disease virus (bdv)-induced model of autism: application to vaccine safety test design. | 2002 | 12142944 | |
| infectious and immune factors in neurodevelopmental damage. | 2002 | 12142943 | |
| crystallization and preliminary x-ray analysis of the matrix protein of borna disease virus. | the matrix protein m of borna disease virus (bdv) is associated with the inner viral membrane and is thought to be a mediator between the nucleocapsid and the lipid-containing envelope in stabilizing the virus shape. the full-length bdv-m gene encoding a 16 kda protein was expressed in escherichia coli. m was purified to homogeneity and crystallized by the sitting-drop vapour-diffusion method. the crystals of m belong to the space group i432, with unit-cell parameters a = b = c = 144.6 a, and di ... | 2002 | 12136159 |
| characterization of the nuclear localization signal of the borna disease virus polymerase. | borna disease virus (bdv) is a nonsegmented negative-strand rna virus that replicates and transcribes its genome in the nucleus of infected cells. bdv proteins involved in replication and transcription must pass through the nuclear envelope to associate with the genomic viral rna. the rna-dependent rna polymerase (l) of bdv is postulated to be the catalytic enzyme of replication and transcription. we demonstrated previously that bdv l localizes to the nucleus of bdv-infected cells and l-transfec ... | 2002 | 12134049 |
| effects of genetic background on neonatal borna disease virus infection-induced neurodevelopmental damage. ii. neurochemical alterations and responses to pharmacological treatments. | the gene-environment interplay is thought to determine variability in clinical conditions and responses to therapy in human neurodevelopmental disorders. studying abnormal brain and behavior development in inbred strains of rodents can help in the identification of the complex pathogenic mechanisms of the host-environment interaction. this paper is the second one in a series of the two reports of the use of the borna disease virus (bdv) infection model of neurodevelopmental damage to characteriz ... | 2002 | 12106671 |
| effects of genetic background on neonatal borna disease virus infection-induced neurodevelopmental damage. i. brain pathology and behavioral deficits. | the pathogenic mechanisms of gene-environment interactions determining variability of human neurodevelopmental disorders remain unclear. in the two consecutive papers, we used the neonatal borna disease virus (bdv) infection rat model of neurodevelopmental damage to evaluate brain pathology, monoamine alterations, behavioral deficits, and responses to pharmacological treatments in two inbred rat strains, lewis and fisher344. the first paper reports that despite comparable virus replication and d ... | 2002 | 12106670 |
| neuroprotection and reduced proliferation of microglia in ribavirin-treated bornavirus-infected rats. | in a rat model of borna disease, intracerebral ribavirin caused clinical improvement without changes in virus titer or nucleic acid. levels of microglia and infiltrating cd4 and cd8 cells were decreased, despite increases in mrnas encoding interleukin-1beta (il-1beta), il-10, and gamma interferon in the brain. intracerebral ribavirin may reduce morbidity through effects on microglia cell proliferation. | 2002 | 12069992 |
| demonstration of continuously seropositive population against borna disease virus in misaki feral horses, a japanese strain: a four-year follow-up study from 1998 to 2001. | borna disease virus (bdv)-specific antibodies were monitored in misaki feral horses annually for 4 years using an electrochemiluminescence immunoassay (eclia). among 130 horses examined, 35 (26.9%) with an eclia count above 1000 once or more were judged as bdv seropositive. throughout the study period, p24 antibodies were more frequent than p40 antibodies in almost all positive animals. among the 35 seropositive horses, the eclia count was consistently high in 12 cases. eleven horses seroconvert ... | 2002 | 12069079 |
| high-avidity human serum antibodies recognizing linear epitopes of borna disease virus proteins. | the recent observation that borna disease virus (bdv)-reactive antibodies from psychiatric patients exhibit only low avidity for bdv antigen called into question their diagnostic value and raised the possibility that antigenically related microorganisms or self antigens caused the production of these antibodies. we further characterized the specificity of these antibodies. | 2002 | 12062882 |
| borna disease in a dog in japan. | borna disease (bd) was diagnosed in a 3-year-old male welsh corgi suffering from a severe and acute progressive disorder of the central nervous system. histopathologically, neuronal lesions were characterized by a non-suppurative encephalomyelitis dominated by large perivascular cuffs consisting of lymphocytes, macrophages and plasma cells; also present were inflammatory cell infiltrates in the neural parenchyma, neuronophagia and focal gliosis. strong immunolabelling with bd virus (bdv) p40 ant ... | 2002 | 12056780 |
| upregulation of chemokine receptor gene expression in brains of borna disease virus (bdv)-infected rats in the absence and presence of inflammation. | infection of adult rats with borna disease virus (bdv) causes cd8 t cell-mediated meningoencephalitis. previously, we described a complex pattern of chemokine gene expression in the central nervous system (cns) of such rats. we now found that expression of chemokine receptor genes cxcr3, ccr5, cx(3)cr1, and cxcr4 was also upregulated, which is in agreement with the predominance in brains of adult infected rats of t cells and monocytes/macrophages that express these receptors. in contrast to thes ... | 2002 | 12053272 |
| 1-beta-d-arabinofuranosylcytosine inhibits borna disease virus replication and spread. | borna disease virus (bdv) is a nonsegmented, negative-strand rna virus that causes neurological diseases in a variety of warm-blooded animal species. there is general consensus that bdv can also infect humans, being a possible zoonosis. although the clinical consequences of human bdv infection are still controversial, experimental bdv infection is a well-described model for human neuropsychiatric diseases. to date, there is no effective treatment against bdv. in this paper, we demonstrate that t ... | 2002 | 12021360 |
| immunopathology and immunoprotection in cns virus infections: mechanisms of virus clearance from the cns. | 2002 | 12014188 | |
| borna disease: current knowledge and virus detection in france. | for over two centuries, borna disease (bd) has been described as a sporadically occurring infectious meningoencephalomyelitis affecting horses and sheep in central europe. over the last decade, the bd epidemiology has been discussed. firstly, its geographical distribution seems larger than what was previously thought. secondly, the disease can affect a large number of warm-blooded animal species, including humans. the aetiological agent is the boma disease virus (bdv), an enveloped, nonsegmented ... | 2002 | 11944803 |
| molecular and cellular biology of borna disease virus infection. | borna disease virus (bdv) is a noncytolytic, neurotropic rna virus that causes neurobehavioral disorders in a wide variety of warm-blooded animals. recent evidence has revealed that bdv uses a unique strategy in its transcription and replication and directly affects cellular functions of infected central nervous systems. bdv research will provide new insights not only into the biology of neurotropic rna virus but also into neuropsychiatry. | 2002 | 11932200 |
| varied persistent life cycles of borna disease virus in a human oligodendroglioma cell line. | borna disease virus (bdv) establishes a persistent infection in the central nervous system of vertebrate animal species as well as in tissue cultures. in an attempt to characterize the life cycle of bdv in persistently infected cultured cells, we developed 30 clones by single-cell cloning from a human oligodendroglioma (ol) cell line after infection with bdv. according to the percentage of cells expressing the bdv major proteins, p40 (nucleoprotein) and p24 (phosphoprotein), the clones were clas ... | 2002 | 11907227 |
| a single amino acid is critical for the expression of b-cell epitopes on the helicase domain of the pestivirus ns3 protein. | truncated ns3 proteins, expressed by recombinant baculoviruses, were used to investigate the location of conserved b-cell epitopes on this non-structural bovine viral diarrhoea virus (bvdv) protein. a goat anti-pestivirus antiserum, and a panel of anti-ns3 monoclonal antibodies, including the bvdv-1 specific antibody p1d8, were used to verify the presence or absence of the epitopes. interestingly, the monoclonal antibodies reacted only with the truncated protein encompassing the helicase domain ... | 2002 | 11900844 |
| detection of antibodies to borna disease virus (bdv) in turkish horse sera using recombinant p40. brief report. | the nucleoprotein of borna disease virus (bdv-p40) was produced in a baculovirus expression system using sf9 cells. the purity and specificity of the recombinant p40 was confirmed by sds-page and immunoblotting. the recombinant p40 was used in an elisa to screen horse sera in turkey. for this, 323 horses from selected cities in the marmara region of turkey were examined clinically and serum was collected from each. all horses were clinically healthy except for a few with wounds on the skin. anti ... | 2002 | 11890534 |
| bornaviruses. | 2002 | 11885951 | |
| borna disease virus infection in psychiatric patients: are we on the right track? | animals infected with borna disease virus (bdv) typically present with neurological dysfunction including behavioural abnormalities. seroepidemiological surveys suggested that bdv infection can occur in human beings and is associated with mental disorders. partly contradictory results from studies employing rt-pcr and serological screening led to debate over whether bdv can infect people at all. critical evaluation of available data led to doubts about the diagnostic value of rt-pcr-based test r ... | 2001 | 11871411 |
| borna disease virus-induced accumulation of macrophage migration inhibitory factor in rat brain astrocytes is associated with inhibition of macrophage infiltration. | to test the hypothesis that macrophage migration inhibitory factor (mif) plays a role in macrophage invasion during virus-induced encephalitis, we analyzed the expression and cellular localization of mif in the borna disease virus (bdv)-infected rat brain, monitored monocyte/macrophage infiltration, and evaluated the influence of anti-inflammatory treatment with dexamethasone. mif mrna expression was restricted to neurons and remained unchanged after bdv infection or after dexamethasone treatmen ... | 2002 | 11870869 |
| borna disease virus infection of the neonatal rat: developmental brain injury model of autism spectrum disorders. | autism spectrum disorders (asd) have been the focus of a great deal of research and clinical speculation. this intense interest relates to both the perplexing pathogenesis and devastating consequences of these disorders. one of the obstacles to understanding the pathogenesis of autism and its efficient treatment has been the paucity of animal models that could be used for hypotheses-driven mechanistic studies of abnormal brain and behavior development and for the pre-clinical testing novel pharm ... | 2002 | 11861216 |
| [detection of anti-borna disease virus antibodies in patients hospitalized in psychiatric hospitals located in the mid-western region of poland]. | borna disease virus (bdv) is single stranded rna virus, which may infect a wide range of animal species. manifestations of the experimental bdv infection show some resemblance to psychopathological symptoms of mental disorders in humans. several reports suggest the higher prevalence of anti-bdv antibodies in psychiatric patients than in healthy controls. however, the seroprevalence of anti-bdv antibodies varied due to the different serological methods used in the previous studies. electrochemilu ... | 2001 | 11842613 |
| [new infectious diseases]. | 2001 | 11820173 | |
| borna disease in a heifer in japan. | 2002 | 11817858 | |
| molecular biology of borna disease virus and persistence. | borna disease virus (bdv) causes central nervous system (cns) disease that is frequently manifested by behavioral abnormalities. recent evidence indicates that the natural host range and geographic distribution of bdv is wider than originally thought bdv has been molecularly characterized as a non-segmented, negative single-stranded (nns) rna virus. its genome (ca 8.9 kb), the smallest among known nns rna viruses, has an organization similar to that of other members of the order mononegavirales. ... | 2002 | 11815302 |
| the immunopathogenesis of borna disease virus infection. | borna disease virus (bdv) infection represents an excellent model system to study immunopathological mechanisms based on a t cell-mediated immune reaction in the central nervous system. the single-stranded rna borna disease virus, a member of bornaviridae in the order of mononegavirale, lacks cytopathogenicity both in vitro and in vivo. after experimental infection bdv causes a persistent infection of the central nervous system and induces borna disease, an immune-mediated encephalomyelitis. the ... | 2002 | 11815301 |
| borna disease virus and infection in humans. | borna disease virus (bdv) is a nonsegmented, negative-, single-stranded, highly neurotropic rna virus with noncytolytic replication in the central nervous system. this virus causes neurological and behavioral disturbances primarily in horses and sheep, in addition to a variety of other vertebrate animal species and in laboratory animal models. bdv is now gaining much of the research attention, because the disturbances seen in animals resemble those of neuropsychiatric disorders in humans. these ... | 2002 | 11815287 |
| characterization of an unusual importin alpha binding motif in the borna disease virus p10 protein that directs nuclear import. | nuclear import of many cellular and viral proteins is mediated by short nuclear localization signals (nls) that are recognized by intracellular receptor proteins belonging to the importin/karyopherin alpha and beta families. the primary structure of nls is not well defined, but most contain at least three basic amino acids and harbor the relative consensus sequence k(k/r)x(k/r). we have studied the nuclear import of the borna disease virus p10 protein that lacks a canonical oligobasic nls. it is ... | 2002 | 11796712 |
| prevalence of circulating antibodies to p10, a non-structural protein of the borna disease virus in cats with ataxia. | japanese domestic cats were surveyed for circulating antibodies to the plo and p24 proteins of the borna disease virus (bdv) by western blotting. twenty-four of 52 cats (46.2%) with ataxia and other neurologic symptoms of unknown cause were positive for antibodies to bdv p10 and/or p24. in contrast, cats without neurological symptoms gave a significantly lower prevalence of anti-bdv antibodies to p10 and/or p24 (36 of 152 cats, 23.7%). thirty specific pathogen-free (spf) cats tested as controls ... | 2001 | 11789604 |
| detection of antibodies to borna disease virus in turkish cats by using recombinant p40. | recombinant p40 produced by baculovirus was used in an elisa to screen samples of serum taken from 80 cats in istanbul. the sera were also analysed for feline immunodeficiency virus (fiv) and feline leukaemia virus (felv). antibodies to borna disease virus- (bdv) p40 were detected in 34 (42-5 per cent) of the 80 cats. seventy-three per cent of the sera which were positive for fiv and 26 per cent of the sera which were negative for fiv had antibodies to bdv. there was no difference in the percent ... | 2001 | 11764326 |
| nosological study of borna disease virus infection in race horses. | to investigate the prevalence of diseases in the borna disease virus (bdv) antibody positive race horses, we undertook seroepidemiological studies of bdv infection on 125 culled race horses in hokkaido, japan. the serological study disclosed the presence of antibodies only to bdv-p40 or -p24 in 19.2% (24/125) and 3.2% (4/125) of culled horses, respectively. antibodies to both bdv-p40 and -p24 were found in 24.0% (30/125) of these horses. of particular note was the finding that locomotorium disor ... | 2002 | 11750144 |
| cortical cholinergic decline parallels the progression of borna virus encephalitis. | borna disease virus (bdv)-induced meningoencephalitis is associated with the dysfunction of the cholinergic system. temporal development of this cholinergic decline during pre-encephalitic and encephalitic stages of bdv infection remains however elusive. changes in choline acetyltransferase (chat) and acetylcholinesterase (ache) activities were therefore determined in the cerebral cortex, hippocampus, striatum, amygdala and cholinergic basal forebrain nuclei (chbfn) of rats infected with bdv. im ... | 2001 | 11726791 |
| identification of pestiviruses contaminating cell lines and fetal calf sera. | three pestiviruses in fetal calf serum (fcs), a pestivirus in porcine st cell line, and two pestiviruses in two of five bovine cell lines were detected by rt-pcr method employing panpestivirus primers selected from the 5'-non-coding region (5'-ncr). the 288 bp products were sequenced in both directions. to identify these pestiviruses, their nucleotide sequences and those of reference pestiviruses were used for construction of a dendrogram. three viruses present in fcs and a virus present in the ... | 2001 | 11719986 |
| open reading frame iii of borna disease virus encodes a nonglycosylated matrix protein. | the open reading frame iii of borna disease virus (bdv) codes for a protein with a mass of 16 kda, named p16 or bdv-m. p16 was described as an n-glycosylated protein in several previous publications and therefore was termed gp18, although the amino acid sequence of p16 does not contain any regular consensus sequence for n glycosylation. we examined glycosylation of p16 and studied its membrane topology using antisera raised against peptides, which comprise the n and the c termini. neither an n- ... | 2001 | 11711600 |
| high-dose borna disease virus infection induces a nucleoprotein-specific cytotoxic t-lymphocyte response and prevention of immunopathology. | experimental borna disease virus (bdv) infection of rats and natural infection of horses and sheep leads to severe central nervous system disease based on immunopathological pathways. the virus replicates slowly, and the cellular immune response results in immunopathology. cd8(+) t cells exert effector cell functions, and their activity results in the destruction of virus-infected cells. previously, oldach and colleagues (d. oldach, m. c. zink, j. m. pyper, s. herzog, r. rott, o. narayan, and j. ... | 2001 | 11689651 |
| an improved protocol for measuring cytotoxic t cell activity in anatomic compartments with low cell numbers. | the study of target cell lysis and cytokine production are valuable tools to characterize antigen-specific t and nk cell function during virus infections. after localized infections in compartments such as the lung or the brain, however, cell numbers isolated from these organs are too low to perform standard assays with individual mice. here, we report a few simple modifications of the classical 51cr release assay allowing reduction of the number of required effector cells by a factor of 10 with ... | 2001 | 11687249 |
| conservation of coding potential and terminal sequences in four different isolates of borna disease virus. | we determined the complete nucleotide sequences of two poorly characterized strains of borna disease virus (bdv) and compared them to reference strains v and he/80. strain h1766 was almost 98% and 95% identical to strains v and he/80, respectively, whereas strain no/98 was only about 81% identical to both reference strains. in contrast to earlier reports, we found an additional a residue at the extreme 3'-end of the single-stranded rna genome in all four bdv strains. the exact numbers of nucleot ... | 2001 | 11602780 |
| infectobesity: obesity of infectious origin. | in the u.s., the prevalence of obesity increased by 30% from 1980 to 1990, and this increase appears to be continuing. although obesity has multiple etiologies, an overlooked possibility is obesity of an infectious origin. six pathogens are reported to cause obesity in animals. canine distemper virus was the first virus reported to cause obesity in mice, followed by rous-associated virus-7, an avian retrovirus, which has been shown to cause stunting, obesity and hyperlipidemia in chickens. next, ... | 2001 | 11584109 |
| cd8(+) t lymphocytes mediate borna disease virus-induced immunopathology independently of perforin. | perforin-mediated lysis of target cells is the major antiviral effector mechanism of cd8(+) t lymphocytes. we have analyzed the role of perforin in a mouse model for cd8(+) t-cell-mediated central nervous system (cns) immunopathology induced by borna disease virus. when a defective perforin gene was introduced into the genetic background of the borna disease-susceptible mouse strain mrl, the resulting perforin-deficient mice developed strong neurological disease in response to infection indistin ... | 2001 | 11581414 |
| wild birds as a possible natural reservoir of borna disease virus. | the natural reservoir of borna disease virus (bdv) is unknown. in this paper, we show that mallards (anas platyrhyncos) and jackdaws (corvus monedula) can be subclinically infected carriers of this virus. from faecal samples collected at a bird pond, we were able to amplify fragments of the bdv p24 and p40 genes. following cloning and sequencing, a phylogenetic analysis revealed that these birds carry strains of bdv closely related to but distinct from the reference strains bdv v and he/80. to o ... | 2001 | 11561971 |
| borna disease virus rna is absent in chronic multiple sclerosis. | 2001 | 11558804 | |
| detection of anti-borna disease virus antibodies from cats in asian countries, japan, philippines and indonesia using electrochemiluminescence immunoassay. | anti-borna disease virus (bdv) antibodies were detected from cats in japan, philippines and indonesia by using electrochemiluminescence immunoassay. positive rates were 3.1%, 3.8% and 2.0% in japan, philippines and indonesia, respectively. there was no differences in the positive rate of anti-bdv antibodies between male and female cats and among habitats. while, a significantly (p<0.05) higher prevalence (6.5%) was found in the oldest age group (more than 6 years) cats. | 2001 | 11558551 |
| the central nervous system inflammatory response to neurotropic virus infection is peroxynitrite dependent. | we have recently demonstrated that increased blood-cns barrier permeability and cns inflammation in a conventional mouse model of experimental allergic encephalomyelitis are dependent upon the production of peroxynitrite (onoo(-)), a product of the free radicals no* and superoxide (o2*(-)). to determine whether this is a reflection of the physiological contribution of onoo(-) to an immune response against a neurotropic pathogen, we have assessed the effects on adult rats acutely infected with bo ... | 2001 | 11544340 |
| [the neuropathogenesis of borna disease virus infection]. | borna disease virus(bdv) is a noncytolytic, neurotropic rna virus that causes a disease of the central nervous system(cns) in several vertebrate species, including horses, sheep, cats and ostriches. epidemiological studies using peripheral blood or brain samples revealed that bdv can infect humans and that it may be related with certain neuropsychiatric disorders. the unique genetic and biological properties of bdv indicate that bdv develops a persistent infection in the cns. furthermore, a line ... | 2001 | 11519168 |
| borna disease virus infection in fiv-positive cats in germany. | 2001 | 11517984 | |
| experimental infection of mice with borna disease virus (bdv): replication and distribution of the virus after intracerebral infection. | to develop an animal model resembling natural asymptomatic borna disease virus (bdv) infections, bdv he/80 rat brain homogenate was passaged four times in adult sjl/j mice. within 12 months of observation, mice did not develop overt signs of disease. nucleotide sequencing of the rat isolate and the mouse isolates at the fourth passage revealed no difference in the deduced amino acids. viral rna was found in brain, heart, kidney, lung, liver, and urinary bladder. infectious virus was isolated fro ... | 2001 | 11517401 |
| evidence of borna disease virus genome detection in french domestic animals and in foxes (vulpes vulpes). | borna disease virus (bdv) is an enveloped, non-segmented negative-stranded rna virus which belongs to the bornaviridae family. bdv is an aetiological agent of encephalitis in horses, sheep and several other vertebrate species. in order to extend our knowledge about the presence of bdv in france, a study based on bdv rna detection by rt-nested-pcr was done with 196 animal tissues: 171 brain samples collected from different animal species (75 horses, 59 foxes, 31 cattle, 4 dogs, 1 sheep, 1 roe dee ... | 2001 | 11514730 |