Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
rinderpest vaccination responses among calves and adult cattle in uganda monitored using elisa assay.responses to the plowright rinderpest vaccine by 43 calves and 70 adult cattle in uganda in 1990, through the production of igg antibodies, were monitored for 4 weeks using the elisa assay. 80% of the calves were seronegative before vaccination of which 32% remained seronegative and 68% subsequently seroconverted at 2 weeks postvaccination. 20% of the calves were seropositive before vaccination but registered a decline during the 2nd and 4th weeks postvaccination. 50% of adult ankole cattle sero ...19938281740
evidence for different lineages of rinderpest virus reflecting their geographic isolation.sequence analysis of part of the fusion protein gene from recent isolates of rinderpest virus revealed that distinct lineages of the virus exist which reflect the geographical location of their isolation in africa and asia. current strains circulating in kenya and sudan were most similar, both in terms of nucleotide sequence and pathogenic nature, to viruses isolated in egypt and in nigeria in 1983/1984 and they were quite distinct from an east african isolate (rbt-1) from the 1960s. two older i ...19938277286
dipstick enzyme immunoassay for rinderpest antibody in cattle.a dipstick enzyme immunoassay (elisa) has been standardized for the detection of rinderpest antibodies. one hundred and thirty bovine serum samples were analysed by the dipstick elisa method and the results compared with the conventional plate elisa method. the sensitivity was found to be similar in both methods. the dipstick elisa does not require expensive micro-plates and an elisa reader, and is recommended for use in field laboratories where the qualitative detection of rinderpest antibodies ...19938273283
biological properties of phocine distemper virus and canine distemper virus.morbilliviruses constitute a major threat to the health of animal and man. to date the morbillivirus genus in the paramyxoviridae family comprises five established members, namely canine distemper virus (cdv), phocine distemper virus (pdv), measles virus (mv), rinderpest virus (rpv), and peste-des-petits-ruminants virus (pprv). in addition, morbillivirus candidates infecting aquatic mammals were recently discovered. the present review on the biology of morbilliviruses focuses on knowledge gained ...19938268007
cloning and expression of the nucleocapsid gene of virulent kabete o strain of rinderpest virus in baculovirus: use in differential diagnosis between vaccinated and infected animals.rinderpest (rp) is a viral disease of ruminants with > 95% morbidity and mortality. we have cloned the cdna of the nucleocapsid (n) gene of the virulent kabete o strain of rinderpest virus (rpvk) and compared its nucleotide and deduced amino acid sequences with those of the n genes of the lapinized strain of rinderpest virus (rpvl), measles virus (mv), and canine distemper virus (cdv). the gene has an open reading frame of 1575 nucleotides and encodes a protein of 525 amino acids with a molecula ...19948259649
[rinderpest eradication and recombinant vaccine]. 19938236832
use of the polymerase chain reaction in differentiating rinderpest field virus and vaccine virus in the same animals.in 1991, a disease with clinical signs indicative of rinderpest was reported in yaks in the former soviet union, near the border with mongolia. at the peak of the epizootic, mortality among affected yaks was 32-42% in adults and 65% in animals less than one year old. pathological samples were examined independently at two institutes in russia. both institutes confirmed the presence of rinderpest using complement fixation, agar gel diffusion and immunoassays. since vaccination had been initiated ...19938219336
rinderpest and peste des petits ruminants-like disease in the al-ain region of the united arab emirates.the author demonstrates that rinderpest prevailed among the cattle population in the al-ain region of the united arab emirates from 1987 to 1989. a total of twenty-four outbreaks of rinderpest were recorded during the study period. similarly, evidence of the peste des petits ruminants (ppr) or a ppr-like disease was regularly encountered in small ruminants in a total of forty-one outbreaks.19938219335
protection of goats against peste des petits ruminants with a vaccinia virus double recombinant expressing the f and h genes of rinderpest virus.peste des petits ruminants (ppr) is a viral disease of goats and sheep characterized by necrotizing and erosive stomatitis, enteritis and pneumonia. the causative agent, pprv, is a member of the family paramyxoviridae and the genus morbillivirus. other members of the genus include rinderpest (rpv), measles, canine distemper and phocid distemper viruses. ppr has a very high rate of morbidity and mortality, and effective control of this disease is of economic importance in africa, asia and the mid ...19938212844
distribution of antigen in cattle infected with rinderpest virus.five holstein heifers (approximately 8 months of age and weighing 225-275 kg) were inoculated subcutaneously with 1,000 tcid50 of rinderpest virus, virulent kabete o strain. they become clinically ill 2 to 5 days post-inoculation, with fever (40 c to 41.5 c), conjunctivitis, and diarrhea. all were euthanatized when moribund at 6 days postinoculation. the following tissues were collected in formalin, embedded in paraffin, and subsequently subjected to histopathologic and immunohistochemical exami ...19948203082
detection of anti-dna antibody responses in rabbits infected with rinderpest virus by elisa.elisa was standardized to detect anti-dna antibodies (adabs) in sera of rabbits infected with rinderpest virus (rpv). these antibodies were found in late (28th and 36th day post inoculation (dpi)) sera of rabbits infected with rabbit virulent lapinized rpv, but not in early (7th dpi) sera of the same rabbits. adabs were not found either in early or late sera of rabbits infected with attenuated rbok strain of rpv. rabbit anti-rpv hyperimmune serum was also found to be positive for adabs. detectio ...19938184791
cloning and sequence analysis of the matrix (m) protein gene of rinderpest virus and evidence for another bovine morbillivirus.we have cloned and sequenced the entire m gene of the vaccine strain of rinderpest virus and that of the virulent kabete "o" strain from which it was derived. the sequences of these two genes are essentially identical (99% at the nucleotide level), but were very different from a previously published kabete o m gene sequence (m. limo and t. yilma, 1990, virology 175, 323-327). inspection of the nucleotide and deduced amino acid sequences of known morbillivirus m genes showed that the earlier sequ ...19948128616
the detection of antibodies against peste des petits ruminants virus in cattle, sheep and goats and the possible implications to rinderpest control programmes.monoclonal antibody-based competitive elisa (c-elisa) have been used for the specific measurement of antibodies to both rinderpest and peste des petits ruminants (ppr) viruses in cattle, sheep and goats. examination of serum samples from sheep and goats in gambia, before and after vaccination with rinderpest vaccine, suggested that antibodies to ppr virus could prevent an immune response to the rinderpest vaccine. cattle sera from nigeria and ghana showed a high prevalence of antibody against pp ...19948119361
comparison of the pathogenicity of rinderpest virus in different strains of rabbits.pathogenicity of the lapinized nakamura-iii (l) strain of rinderpest virus (rpv) was examined in four strains of rabbits consisting of two inbred strains (nw-nibs and duy-nibs) and two outbred strains maintained in closed colony (nw-nibs and jw-nibs) using a marmoset lymphoblastoid cell line, b95a cell-passaged virus and tissue homogenates of virus-infected rabbits. the cell culture virus was found to maintain virulence for rabbits of both closed colony and inbred nw-nibs strain similar to the h ...19938117822
viral antigen distribution in organs of cattle experimentally infected with rinderpest virus.the distribution of viral antigen in various organs of four approximately 10-month-old castrated male friesian cattle experimentally infected with a highly virulent strain of rinderpest virus was studied. a monoclonal antibody with genus-specific reactivity for morbilliviruses was applied in an indirect immunoperoxidase method performed on formalin-fixed, paraffin-embedded tissue sections. rinderpest viral antigen was located mainly in the cytoplasm of the epithelial cells of the digestive, resp ...19938116148
visual detection of rinderpest viral antigens on surface. 19938105648
characteristics of an attenuated vaccinia virus strain, lc16m0, and its recombinant virus vaccines.this article reviews studies concerning the characteristics of a vaccinia virus strain, lc16m0, and its recombinant virus vaccines. the lc16m0 strain is one of several temperature-sensitive and further attenuated variants derived from the lister (elstree) strain of vaccinia virus, which has a proven safety record in human populations. several types of recombinant vaccinia viruses expressing a foreign antigen gene from a pathogenic virus have been constructed using the lc16m0 strain as a vector, ...19948091843
recombinant capripoxvirus expressing the hemagglutinin protein gene of rinderpest virus: protection of cattle against rinderpest and lumpy skin disease viruses.a cdna clone containing the complete coding sequence of the hemagglutinin (h) protein gene of the rbok vaccine strain of rinderpest virus, under the control of the vaccinia late promoter p11, was inserted by homologous recombination into the thymidine kinase gene of the ks-1 strain of capripoxvirus. the recombinant virus produced authentic h protein as judged by its electrophoretic mobility, transport to the cell surface of infected lamb testis cells, and reactivity with monoclonal antibodies sp ...19948091673
genomic heterogeneity of rice dwarf phytoreovirus field isolates and nucleotide sequences of variants of genome segment 12.electrophoretic profiles of the dsrnas of field isolates of rice dwarf virus (rdv) were compared with those of an isolate maintained at hokkaido university (rdv-h). unexpectedly, the genomic dsrnas of most of the field isolates showed distinct electrophoretic mobility profiles. this was the case even among isolates from the same region. genome segment 12 (s12) from some variants migrated faster than s12 from rdv-h. these rnas were converted to full-length cdnas and sequenced. s12 from all the va ...19948046386
immunodetection of rice dwarf phytoreoviral proteins in both insect and plant hosts.peptides encoded by truncated (s1 and s2) or full-length (s3 to s11) cdnas of 11 of the 12 rice dwarf phytoreovirus (rdv) genome segments were expressed in a baculovirus vector system. antibodies raised against each of the expressed peptides were used as probes to detect the authentic proteins encoded by the rdv open reading frame. polypeptides identified as gene products of s1 to s11 in both rdv-infected rice leaf and leafhopper (nephotettix cincticeps) homogenates were the p1 minor core (170 k ...19948009852
nucleotide sequence comparisons of the fusion protein gene from virulent and attenuated strains of rinderpest virus.we have cloned and sequenced the entire fusion (f) protein gene of the rbok vaccine strain of rinderpest virus and the coding regions for the f genes of two mild field isolates of the virus from africa. analysis of the nucleotide and the predicted amino acid sequences showed that the vaccine virus was more than 99% identical in the protein coding region to the virulent kabete o strain from which it was derived, whereas the field isolates differed by 10 to 12% from each other and from the vaccine ...19947996154
circulating immune complexes in rabbits surviving rinderpest virus infection.circulating immune complexes (cics) precipitated from the sera of rabbits recovered from infection with rabbit virulent (lapinized) rinderpest virus (rpv) were found to contain rpv antigen and rabbit igg. the cics persisted for at least 77 days post infection. the cics and the antigen isolated from them by chromatography on sepharose-6b were found to be non-infectious. rpv-specific antibodies could be detected in the antisera raised in rabbits against the cics and the isolated antigen. furthermo ...19947976861
detection of novel rna viruses: morbilliviruses as a model system.recent advances in molecular detection of viral-specific nucleic acid sequences have facilitated the search for pathogenic viruses in clinical samples. primers for the polymerase chain reaction can be targeted to unique or conserved regions of the viral genome. while the design of primers must be based on known sequences, it has been demonstrated in bacteria that amplification with primers which hybridize to highly-conserved regions of the genome can be exploited to detect new agents. by taking ...19947969194
applications of recombinant vaccinia virus for veterinary vaccines. 19947958475
progress in the development of a heat-stable recombinant rinderpest vaccine using an attenuated vaccinia virus vector.rinderpest is a fatal infectious disease of cattle and buffalo, and is prevalent in many parts of the developing world. eradication campaigns are at present under way in africa, the middle east and south asia. as these regions have very hot climates, it is difficult and expensive to establish reliable cold chains to deliver heat-sensitive vaccines and, for this reason, many previous vaccination/eradication campaigns have been ineffective. to overcome the problem of vaccine heat-lability, a recom ...19947949348
sequencing and analysis of the nucleocapsid (n) and polymerase (l) genes and the terminal extragenic domains of the vaccine strain of rinderpest virus.the nucleocapsid (n) and polymerase (l) genes of the vaccine strain of rinderpest, and the 5' and 3' terminal domains of the genome have been sequenced. together with previously published data, this completes the sequence of the entire genome of rinderpest virus. the viral genome is 15,881 bases in length, similar to that of measles virus and slightly longer than that of canine distemper virus. the l gene is identical in length to that of measles virus, encoding a 2183 amino acid protein with a ...19957897350
protection of cattle against rinderpest and lumpy skin disease with a recombinant capripoxvirus expressing the fusion protein gene of rinderpest virus.cattle were protected against challenge with rinderpest and lumpy skin disease viruses by vaccination with a recombinant capripoxvirus containing the fusion protein (f) gene of rinderpest virus. the minimum protective immunising doses for rinderpest and lumpy skin disease were 5.5 x 10(4) plaque forming units (pfu) and 1.5 x 10(3) pfu, respectively.19947880246
effect of ammonium chloride on multiplication of rinderpest virus in vero cells.ammonium chloride, a lysosomotropic weak base, inhibited replication of rinderpest virus in vero cells. the inhibition of replication was dose-dependent and the minimum effective dose of ammonium chloride was determined as 5-10 mmol/l. the fusion efficiency and the yield of both cell-free and cell-associated virus were reduced in the presence of the inhibitor. western blot analysis of rinderpest virus-infected vero cells revealed that synthesis of two virus-induced polypeptides were affected by ...19947817898
live virus vaccines for use in animals. 19957796966
vaccinia virus recombinant vaccines for rinderpest. 19957796955
capripoxvirus-based multivaccines. 19957796952
humoral antibody response in animals infected with virulent rinderpest virus.humoral antibody responses in cattle or rabbits infected with virulent rinderpest virus or lapinised rinderpest virus respectively were assessed. rinderpest specific antibodies could be first detected 6 days post-infection. no correlation could be established between antibody response and the course of the disease in infected animals during the early stages of infection. the animals with fatal infection either did not respond or had a transient antibody response. a gradual increase in antibody t ...19957770948
protection of goats against peste des petits ruminants with recombinant capripoxviruses expressing the fusion and haemagglutinin protein genes of rinderpest virus.goats were protected against a lethal challenge of peste des petits ruminants (ppr) virus following vaccination with a recombinant capripoxvirus containing either the fusion (f) gene of rinderpest virus or the haemagglutinin (h) gene of rinderpest virus. the h gene recombinant produced high titres of neutralizing antibody to rinderpest virus in the vaccinated goats, whereas the f gene recombinant failed to stimulate detectable levels of neutralizing antibody. a similar response to the two recomb ...19957762275
immunohistochemical studies of lymphoid tissues of rabbits infected with rinderpest virus.the pathogenesis of infection with the l-strain of rinderpest virus (rpv) in rabbits was investigated. of several lymphoid tissues examined, those associated with the gut showed the most marked virus growth. the virus titres were maximal 4 days after inoculation but had declined at day 6. the distribution of viral antigen was examined immunohistochemically with the recently established anti-rabbit cd5 monoclonal antibody (moab), which is a pan-t-cell marker, and the anti-rpv-nucleoprotein moab. ...19957722007
development of a competitive elisa for detecting antibodies to the peste des petits ruminants virus using a recombinant nucleoprotein.a competitive elisa based on the reaction between a monoclonal antibody (mab) and a recombinant nucleoprotein of the peste des petits ruminants virus (pprv) was developed. this protein was obtained in large quantities from insect cells infected with a ppr nucleoprotein recombinant baculovirus (n-b). the competitive elisa was compared with the virus neutralisation test (vnt) for detecting specific antibodies to pprv in sheep and goats. the time consuming vnt is the only prescribed test that is ca ...19957709061
identification of epitope expression on the internal proteins of rinderpest virus which is dependent upon virion maturation events.monoclonal antibodies (mab) identified the existence of both maturation-dependent and maturation-independent epitopes on rinderpest virus antigens. the former were divided into (i) post-maturation antigenic determinants, which were dependent upon the maturation of viral antigen into complete virions; and (ii) pre-maturation antigenic determinants, which were only expressed on what appeared to be immature particles before 'budding' into the extracellular environment. epitope expression could be r ...19937679394
differentiation of peste des petits ruminants and rinderpest viruses by neutralisation indices using hyperimmune rinderpest antiserum.six field isolates believed to be rinderpest viruses and 2 known strains of peste des petits ruminants (ppr) viruses were titrated in the presence of normal rabbit serum and with hyperimmune rinderpest antiserum prepared in rabbits. the known ppr viruses had indices less than 10 whereas 4 of the suspect field isolates had indices greater than a hundred. two suspect field isolates had indices less than 20; both were collected from small ruminant wild life and are probably ppr viruses.19957652944
rinderpest virus infection of bovine peripheral blood monocytes.the ability of rinderpest virus (rpv) to replicate in vitro in adherent peripheral blood monocytes and monocyte-derived macrophages under non-stimulation conditions was investigated. when flow cytometry analysis on bovine peripheral blood mononuclear cells (pbmc) was performed, monocytic cells were seen to be targets for infection by the cell culture-attenuated rbok vaccine strain of rpv. viral glycoprotein (h) and nucleoprotein (n) expression in adherent blood monocytes and monocyte-derived mac ...19957595385
high resolution genome typing and genomic reassortment events of rice dwarf phytoreovirus.genomic reassortment of rice dwarf phytoreovirus (rdv) was experimentally demonstrated for the first time in plant reoviruses. combinations of two genomic variants, most of the genomic segments of which could be distinguished by a high resolution polyacrylamide gel electrophoresis, were used to produce genomic reassortants. after artificial mixed injection of two of three isolates (rdv-s, rdv-ai, and rdv-an) into the insect vector nephotettix cincticeps, rice seedlings were sequentially inoculat ...19957571443
differential immunohistochemical staining of peste des petits ruminants and rinderpest antigens in formalin-fixed, paraffin-embedded tissues using monoclonal and polyclonal antibodies. 19947516718
studies on viral interference induced by rinderpest virus: inactivation of tissue culture rinderpest vaccine virus by heat treatment at 56 degrees c and by ultraviolet irradiation. 19807467995
the effect of trypanosoma congolense and t vivax infections on the antibody response of cattle to live rinderpest virus vaccine.infections with trypanosoma congolense or t vivax did not significantly depress the neutralising antibody response of cattle to live rinderpest vaccine when vaccination was carried out eight or 25 days after infection.19807414077
safety of tissue culture rinderpest vaccine in pregnant goats. 19817292614
establishment of a persistently infected cell line with rinderpest virus.persistent infection of rinderpest virus in vero cells was established and designated as vrp34. virus specific antigens were present in nearly 100 per cent of the cells. cytopathic effect (cpe) consisting of syncytium formation and vacuolation is a unique feature of vrp34. spontaneously released virus mainly consisted of non-temperature-sensitive virus populations and was able to initiate persistent infection in both normal vero and rk13 cells. the results indicate that mutation of virus is resp ...19817271459
rinderpest in nigeria. 19817257160
studies on viral interference induced by rinderpest virus: 3. interferon induction by attenuated and virulent strains of rinderpest virus in tissue culture. 19827186805
neutralising antibodies to rinderpest virus in sheep and goats in western kenya. 19827157610
precipitating antibody in sera of goats naturally affected with peste des petits ruminants. 19827101469
microtitre techniques for the assay of rinderpest virus and neutralising antibody.microtitre techniques were compared with conventional tube techniques for their ability to assay rinderpest virus and neutralising antibody to the virus. the microtitre technique was as sensitive and reliable for assaying the virus as the recommended tube technique, using cell suspensions. both of these methods, however, were less sensitive than tube titrations on preformed cell monolayers. the microtitre test was as sensitive as the tube test for detecting and assaying virus neutralising antibo ...19827079607
an enzyme-linked immunosorbent assay for the detection of igg, iga and igm antibodies to rinderpest virus in experimentally infected cattle. 19827043688
microelisa test for detecting antibodies to rinderpest virus antigens.a microplate enzyme-linked immunosorbent assay (elisa) was developed which detected antibodies to a soluble antigen prepared from sonicated rinderpest virus-infected cells. the elisa detected titres of antibody to the virus in the sera of cattle 3 weeks after immunisation with tissue culture rinderpest virus vaccine which were similar to those detected by the virus neutralisation test. the elisa test shows potential as a rapid and economic technique for screening large numbers of sera for antibo ...19817015643
comparative aspects of pathogenicity of measles, canine distemper, and rinderpest viruses. 19806997585
comparison of autoimmunity induction with virulent and attenuated rinderpest virus in rabbits.two strains of rinderpest virus which differ each other in virulence to rabbits were compared in their capacity to produce autoantibodies and their effects on the function of the lymphoid system. the virulent l strain induced two autoantibodies, i.e., antinuclear antibody (ana) and cold hemagglutinating antibody (ha), and suppressed lymphocyte response to phytohemagglutinin and to concanavalin a for at least 4 weeks after infection. the attenuated la strain, on the other hand, failed to induce t ...19816975843
rapid detection of rinderpest virus antigens by counter-immunoelectrophoresis.counter-immunoelectrophoresis was compared with immunodiffusion for its ability to detect rinderpest virus antigens. counter-immunoelectrophoresis detected antigens in lymph node biopsies from all of 9 infected cattle whereas immunodiffusion detected them in 8 only. counter-immunoelectrophoresis was between 4 and 16 times more sensitive than immunodiffusion for detecting rinderpest virus antigens in the tissues of diseased animals and could detect positive reactions within 40 min. counter-immuno ...19806779407
detection of rinderpest virus antigens in vitro and in vivo by direct immunofluorescence.cell-culture attenuated and virulent strains of rinderpest virus (rv) were inoculated on to bovine kidney cell cultures. a direct immunofluorescent antibody test detected rv antigens in cell cultures within one to three days after inoculation whereas rv cytopathic effects usually took three to nine days to develop. cells containing rv antigens were also detected in impression smears and frozen sections of tissues collected from rv infected animals at post mortem examination, and in smears of lym ...19826755594
the use of an enzyme-labelled assay as an aid to reading micro virus-neutralisation tests.an indirect immunoperoxidase technique has been developed for the detection of virus-infected cells after completion of a micro virus-neutralisation test. this allows visual rather than microscopic reading of the plates, which is less time-consuming and does not require a technician skilled in distinguishing between specific viral cytopathic effect and non-specific cytotoxicity. the technique proved to be more sensitive than microscopic reading for the detection of low levels of antibody and cou ...19826754814
the duration of immunity in cattle following inoculation of rinderpest cell culture vaccine.the duration of immunity following a single administration of rinderpest cell culture vaccine, of 90 or more monolayer passages, was studied in e. african zebu (boran) and grade (cross-bred european) cattle. all animals were kept for periods of 6-11 years in rinderpest-free environments; groups of them (in all 23 borans and 10 grades) were then challenged by parenteral or intranasal inoculation of virulent virus or by contact exposure to reacting cattle. nasal excretion of virus was studied dail ...19846736639
a microneutralization test for the detection of rinderpest virus antibodies. 19846531493
the detection of peste des petits ruminants (ppr) virus antigen by agar gel precipitation test and counter-immunoelectrophoresis.the detectability of peste des petits ruminants (ppr) viral antigen in both ante-mortem secretions and necropsy samples from experimentally infected goats was investigated by both the agar gel precipitation test (agpt) and counter-immunoelectrophoresis (cie). viral antigen was detected from 42.6% of the samples tested by the agpt and 80.3% by cie. the detection of viral antigen in a high proportion of the ocular and nasal secretions as well as the faeces and buccal scrapings, particularly from t ...19846512258
the shedding of a virulent kabete o strain of rinderpest virus by cattle.a muguga substrain of the virulent kabete o strain of rinderpest virus was demonstrated in the ocular, nasal, oral and rectal swabs collected from infected cattle. ocular shedding was detected at the onset of viraemia and before the onset of clinical signs whilst virus shedding in nasal, oral and rectal discharges appeared at the same time as lesions. it is suggested that virus isolation from ocular and nasal swabs should be considered in the diagnosis of rinderpest in addition to the other meth ...19846495633
serum-virus simultaneous method of immunisation against peste des petits ruminants.peste des petits ruminants (ppr) hyperimmune serum was prepared in cattle and used to protect goats against ppr. the serum was well tolerated by goats and 8 ml of serum was found to protect goats against 4 ml of pooled virulent ppr virus. a durable active immunity was conferred on goats when serum and virulent ppr virus were given simultaneously. passive immunity lasted for 15 days.19846485097
serological studies with peste des petits ruminants and rinderpest viruses in nigeria.one hundred and ninety-five goat and 67 sheep sera collected from various parts of southern nigeria were screened for neutralising antibodies to both the peste des petits ruminants (ppr) and rinderpest viruses. neutralising antibodies against both viruses were found in the sheep and goat sera examined. parallel titration of samples which neutralised both viruses indicated a primary infection with the ppr virus (pprv). however, some samples which failed to neutralise pprv neutralised the rinderpe ...19846485096
detection of rinderpest antigen by agar gel diffusion and counter-immunoelectrophoresis. 19836408775
rapid diagnosis of rinderpest by countercurrent immuno-electro-osmo-phoresis in nigeria. 19846395580
detection of rinderpest antibodies.rinderpest antibodies were detected by employing the fluorescent antibody test (fat) and the immunoperoxidase test (ipt) and the results were compared with the counterimmuno electrophoresis test (cie). fat was found to be the most sensitive in detecting post-vaccinal antibodies followed by ipt and cie tests.19846385428
use of an enzyme-linked immunosorbent assay for the detection of igg antibodies to rinderpest virus in epidemiological surveys.a comparison was made between an indirect enzyme-linked immunosorbent assay (elisa) and the virus neutralisation test (vnt) for the detection of antibodies to rinderpest virus in field sera. the results did not agree for 6 per cent of the sera tested where 3 per cent of the samples gave elisa positive/vnt negative and 3 per cent gave elisa negative/vnt positive. the latter sera all had high levels of igm antibody, which may indicate animals being at an early stage of infection or detection of a ...19836340173
isolation of peste des petits ruminants virus from the sudan.based on clinical signs, the presence of a rinderpest related antigen and the ability of rinderpest immune serum to prevent virus isolation, rinderpest was diagnosed in two outbreaks in goats in central sudan during 1971 and 1972. two viruses isolated from these goats have been re-examined both serologically and by the inoculation of experimental cattle, sheep and goats. each is now considered to represent the virus of peste des petits ruminants, thereby extending the geographical range of this ...19846200906
studies on viral interference induced by rinderpest virus: 4. induction of circulating interferon and antibodies in buffaloes following inoculation with tissue culture rinderpest vaccine (tcrv) virus. 19826194837
the separation of three antibody populations from anti-poly(a).poly(u) antibodies elicited in mice or rabbits and antigenic features of poly(a).poly(u)).anti-poly(a).poly(u) antibodies in ascitic fluid of ddy mice immunized with poly(a).poly(u)-methylated bovine serum albumin complexes were fractionated into three major antibody populations, ab-1, ab-2 and ab-3, by precipitating with poly(i).poly(c), poly(a).poly(u), and poly(a).2 poly(u), respectively. antibody population one, ab-2, reacted with various double-stranded rnas [poly(i).poly(c), poly(a).poly(u), and rice dwarf virus ribonucleic acid (rdv-rna)] and poly(a).2 poly(u). ab-2 reacted wi ...19826178956
analysis of structural proteins of measles, canine distemper, and rinderpest viruses.serological relationships among measles virus (mv), canine distemper virus (cdv), and rinderpest virus (rv), which constitute morbillivirus subgroup of paramyxoviridae, were investigated by immunoprecipitation and sds-polyacrylamide gel electrophoresis for their major structural proteins, i.e., hemagglutinin (h), nucleocapsid (nc), fusion (f), and matrix (m) proteins. the molecular weights of the four structural proteins of mv and cdv were confirmed to correspond to those previously reported by ...19816174760
production of interferon by rinderpest virus in calf kidney cell cultures. 19816165231
studies on viral interference induced by rinderpest virus: interference and interferon induction by tissue culture rinderpest vaccine (tcrv) virus in vitro. 19806162300
antigenic differences in the hemagglutinin of measles and related viruses. 19816162273
observations on rinderpest in sheep and goats and transmission to cattle. 19676067705
transmission of rinderpest by contact from cattle to sheep and goats. 19676067704
susceptibility of exotic breeds of cattle to rinderpest lapinised and lapinised avianised vaccines. 19676066401
the use of tissue culture rinderpest vaccine for egyptian cattle and water buffaloes. 19676058184
[note on the cultivation of lapinized rinderpest virus in bovine leukocyte cultures]. 19676057337
electron microscopy of cytoplasmic inclusion bodies in cells infected with rinderpest virus. 19676017409
[note on the action of sodium sulfate, magnesium sulfate and magnesium chloride on rinderpest virus adapted to cell culture]. 19666008652
[hemagglutination inhibition reaction with measles antigen in rinderpest. ii. antibody formation after inoculation of various live vaccines in cattle]. 19665994742
identification and control of a rinderpest outbreak in saudi arabia. 19665967349
response of virus diarrhea-mucosal disease-convalescent calves and rinderpest-vaccinated calves to inoculation with heterologous virus. 19665959401
resistance of two bulls against rinderpest without detectable neutralising antibodies in their sera. 19665909635
a secondary effect from the use of caprinised rinderpest virus vaccine on heavily tick-infested cattle. 19635896617
production, evaluation and use of lapinized-avianized rinderpest virus vaccine in egypt. 19645896557
duration of rinderpest immunity in cattle following vaccination with caprinised rinderpest virus. 19655893785
propagation of rinderpest virus in suckling mice and its comparison to murine adapted strains of measles and distemper. 19655882876
[some basic investigations on the rinderpest virus]. 19655881271
response of calves, sheep, and pigs to a cell-culture-modified rinderpest virus. 19655860391
[possibilities and limits of the measles hemagglutination inhibition test in the serology of rinderpest. ii. dissimilarity between the results obtained with this test and the rinderpest virus seroneutralization]. 19695817939
studies on rinderpest culture vaccine. i. some factors affecting virus production. 19695809570
[note on experimental bovine plague in the dromedary]. 19685754444
[duration of rinderpest virus elimination in cattle immunized with inactivated vaccine]. 19685748244
the susceptibility of the one-humped camel (camelus dromedarius) to infection with rinderpest virus. 19685731205
[effect of osmotic pressure on the multiplication of rinderpest virus in cell culture]. 19685713826
19 s neutralizing antibody produced in guinea pigs by rabies virus. 19685712546
role of interferon in protection of rabbits against rinderpest virus. 19685712545
[conservation of lyophilized rinderpest virus]. 19685709381
transmission of rinderpest from goats to cattle in tanzania. 19685693873
Displaying items 401 - 500 of 725