Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| "nonrandom" dna sequence analysis in bacteriophage m13 by the dideoxy chain-termination method. | we describe a rapid "nonrandom" dna sequence analysis procedure that facilitates the nucleotide sequence determination of large contiguous regions of dna. the method consists of cloning a restriction endonuclease fragment of interest into bacteriophage m13 followed by construction of a series of nuclease bal-31 deletion mutants originating from a single site in m13 that is close to the dna insert. determination of the size of the deletion mutant is accomplished by hybridization to a complementar ... | 1982 | 6956859 |
| genes vi, vii, and ix of phage m13 code for minor capsid proteins of the virion. | the minor capsid proteins c and d from phage m13 have been characterized by differential amino acid labeling and amino-terminal sequence analysis. we demonstrate that d protein (mr 12,260) is the product of gene vi, whereas the c component is composed of the products of both gene vii (mr 3580) and gene ix (mr 3650). our data further show that the proteins of genes vi, vii, and ix are not subject to proteolytic processing but are packaged into mature virions as their primary translational product ... | 1981 | 6945579 |
| replication of the plasmid pbr322 under the control of a cloned replication origin from the single-stranded dna phage m13. | the replication origins of viral and complementary strands of bacteriophage m13 dna are contained within a 507-nucleotide intergenic region of the viral genome. chimeric plasmids have been constructed by inserting restriction endonuclease fragments of the m13 intergenic region into the plasmid pbr322. replication of these hybrid plasmids, under conditions not permissive for the plasmid replicon, depends on specific segments of the m13 origin region and on the presence of m13 helper virus. thus m ... | 1980 | 6933512 |
| mechanisms of membrane assembly: effects of energy poisons on the conversion of soluble m13 coliphage procoat to membrane-bound coat protein. | the coat protein (gene 8 product) of coliphage m13 spans the host cell plasma membrane prior to its assembly into extruding virions. it is made as a soluble precursor, termed procoat, with an extra 23 nh2-terminal amino acid residues. we have examined the effect of metabolic poisons on the assembly of procoat into the plasma membrane and its proteolytic conversion to coat protein. protein synthesis and proline uptake were measured to assess the effect of each poison on cellular high-energy phosp ... | 1980 | 6928682 |
| nucleotide-sequence heterogeneity and sequence rearrangements in influenza virus cdna. | double-stranded cdna has been synthesized from influenza virus rna and cloned into derivatives of the bacteriophage m13 for sequence analysis. the characterization of over 200 clones has permitted an analysis both of nucleotide sequence heterogeneity and of clones containing unusual rearrangements of sequence. heterogeneity, due to genetic variability in the rna population and to in vitro synthetic errors, was detected at the low level of one nucleotide difference per 3 700 nucleotides. by contr ... | 1981 | 6895362 |
| characterization and properties of a modified human interferon-alpha containing an additional 18 amino acids at the n-terminus. | a modified human interferon-alpha 2 was produced in escherichia coli cells infected with phage m13 mp7 containing an interferon-alpha gene. after purification by immunochromatography with the monoclonal antibody nk2, the n-terminal amino acid sequence was determined. the n-terminal methionine was absent but an additional sequence of 18 amino acids at the n-terminus was retained. the modified interferon-alpha 2 was indistinguishable from authentic interferon-alpha 2 in its ability to activate nat ... | 1983 | 6875519 |
| proteins encoded near the adenovirus late messenger rna leader segments. | small fragments of adenovirus 2 dna cloned into the single-strand phage m13 were used to select adenoviral messenger rnas transcribed from the r-strand between map positions 16 and 30. cell-free translation of these mrnas produced proteins of 13.5k, 13.6k, and 11.5k, respectively encoded between the first and second segments of the tripartite major late leader, within the "i"-leader segment, and immediately preceding the third leader segment. partial sequence analysis of the 13.6k protein is con ... | 1983 | 6857999 |
| coat protein conformation in m13 filaments, i-forms and spheroids. | circular dichroism studies of the filamentous coliphage m13 were carried out to determine conformational changes in the major capsid protein (the b protein) that occur during contraction of the filaments to i-forms and spheroids. the alpha-helicity of the b protein is somewhat lower in the i-forms than in filaments and much lower in spheroids. this conformational change may explain the increased detergent and lipid solubility of both i forms and spheroids relative to filaments. | 1983 | 6847652 |
| the yeast his3 promoter contains at least two distinct elements. | phenotypic analysis of 65 mutations indicates that the yeast his3 promoter is composed of at least two separate regions of dna. each is necessary, but neither is sufficient for wild-type levels of his3 expression. deletion mutations that destroy either promoter element express his3 poorly or not at all. the upstream element is located between 112 and 155 base pairs before the site of transcriptional initiation (nucleotides -112 to -155). a comparison of derivatives strongly suggests that the dow ... | 1982 | 6760196 |
| an efficient synthetic primer for the m13 cloning dideoxy sequencing system. | the deoxytetradecamer d(aaaacgacggccag) has been shown to be an excellent universal primer for sequence determination of dna cloned into the bacteriophage m13 mp7, mp8, and mp9 series. this new primer offers several advantages over others currently available and it has been used to define the cloning of hinf i fragments of bacteriophage s13 dna into the eco ri site of m13 mp7, utilizing the homologous complementary base pairing of the two restriction sites. of the four possible sequence derivati ... | 1982 | 6753962 |
| a 500-mhz proton nuclear magnetic resonance study of the structure and structural alterations of gene-5 protein-oligo(deoxyadenylic acid) complexes. | the complex of the gene-5 protein of bacteriophage m13 with octadeoxyadenylic acid [d(a)8] has been shown earlier to differ in various respects from the complex with polynucleotides [alma, n. c. m., harmsen, b. j. m., van boom, j. h., van der marel, g., & hilbers, c. w. (1982) eur. j. biochem. 122, 319-326]. in this paper the gene-5 protein-d(a)8 complex is compared with the complex formation between the gene-5 protein and a mixture of longer oligonucleotides, i.e., d(a)25-30. nuclear overhauser ... | 1983 | 6602628 |
| fluorescence studies of the complex formation between the gene 5 protein of bacteriophage m13 and polynucleotides. | 1983 | 6601193 | |
| splicing of adenovirus rna in a cell-free transcription system. | a soluble whole-cell extract prepared accurately from hela cells splices 2-3% of the rna transcribed from a dna template containing the first and second leader exons of late adenovirus rna. the spliced rna was detected by a sensitive technique using hybridization to a single-stranded phage m13 cdna clone, followed by binding to nitrocellulose filters. the identity of the spliced rna was established by rnase t1 and pancreatic rnase two-dimensional peptide mapping. the bond formed during the in vi ... | 1983 | 6577417 |
| a detailed mutational analysis of the eucaryotic trnamet1 gene promoter. | we have isolated phage m13 clones containing the x. laevis trnamet1 gene, each having one or a few c leads to t transitions in the trna coding sequence. nearly every g-c and c-g base pair in the tdna has been mutagenized. the importance of these altered nucleotides in transcription by rna polymerase iii has been assessed by injecting the cloned dnas into frog oocyte nuclei together with alpha-32p-gtp and measuring the synthesis of labeled trnamet1. several g-c and c-g base pairs in the structura ... | 1983 | 6552939 |
| cloned single- and double-stranded dna copies of potato spindle tuber viroid (pstv) rna and co-inoculated subgenomic dna fragments are infectious. | a set of monomeric and oligomeric potato spindle tuber viroid (pstv) specific dna forms representing complete dna copies of the circular pstv rna genome were constructed and cloned in plasmid pbr322 and bacteriophage m13. both single- and double-stranded pstv dnas are capable of initiating viroid replication in mechanically inoculated tomato plants where it normally proceeds via the rna-rna pathway without dna being involved. all dimeric and higher multimeric forms were infectious irrespective o ... | 1984 | 6549294 |
| structure of murine complement component c3. i. nucleotide sequence of cloned complementary and genomic dna coding for the beta chain. | the nucleotide sequence coding for the beta chain of murine c3 was determined from cloned cdna and genomic dna fragments. sonicated subfragments were randomly inserted into the bacteriophage m13 and sequenced using the dideoxynucleotide technique. each nucleotide was sequenced on average six times in these studies. the derived amino acid sequence includes a signal peptide and a tetra-arginine sequence between the beta and alpha subunits in the precursor polypeptide prepro-c3. together with the a ... | 1984 | 6548745 |
| effect of spermine on interaction of dna polymerase alpha from the loach (misgurnus fossilis) eggs with dna. | polyamines (putrescine, spermidine and spermine) cause a marked increase in the activity of the loach misgurnus fossilis dna polymerase alpha on activated (gapped) dna. the stimulatory effect increases in the order: putrescine, spermidine, spermine. kinetic analysis shows that spermine does not change the affinity of the polymerase for dttp, but it decreases the enzyme affinity for dna. the apparent km of the polymerase for activated dna progressively increases from 14 to 1200 microm (nucleotide ... | 1984 | 6548155 |
| replication origin of the bacillus subtilis chromosome determined by hybridization of the first-replicating dna with cloned fragments from the replication origin region of the chromosome. | the replication origin (ori) on the bacillus subtilis genome was determined by the hybridization between the first-replicating dna region and the cloned fragments from the ori region. the first-replicating dna region was labeled specifically by [3h]thymidine in the presence of an inhibitor for dna polymerase during a synchronous initiation of the chromosomal replication by germinating spores starved for thymine, and isolated by a sucrose density gradient centrifugation. most of the labeled dna m ... | 1984 | 6439606 |
| rapid mutational analysis of regulatory loci in escherichia coli k-12 using bacteriophage m13. | a derivative of bacteriophage m13mp8 , designated m13mp8 /p, was prepared in which the promoter and nh2-terminal codons of bacterial genes may be fused to a portion of beta-galactosidase, resulting in an easily scorable phenotype. because transcription from the inserted promoter remains responsive to the host regulatory system, it is simple to screen mutagenized phage for isolates with aberrant regulatory phenotypes and to determine the mutational changes by dideoxy sequence analysis. the feasib ... | 1984 | 6427775 |
| on the processivity of dna replication. | in this paper we describe the nature and importance of processive enzymatic reactions in biological processes. a model is set up to describe the processive synthetic process in dna replication, and experiments are presented to define and test the model, using the components of the t4 phage-coded five-protein (in vitro) dna replication system of alberts. nossal and coworkers. these experiments are performed either with a homogeneous oligo dt-poly da primer-template system, or with a natural prime ... | 1983 | 6400896 |
| different base/base mismatches are corrected with different efficiencies by the methyl-directed dna mismatch-repair system of e. coli. | the efficiency of methyl-directed dna mismatch-repair of e. coli acting in vivo on heteroduplex genomes of phage m13 was found to be strongly dependent on the nature of the base/base mismatch to be corrected. three efficiency classes were characterized:high (t/g, c/a and g/g); intermediate (a/a); and low (g/a, a/g, t/t, c/c, c/t and t/c). methyl-directed dna mismatch repair was lost completely for any type of mismatch in strains carrying either mutl or muts mutations. data obtained with a muth m ... | 1984 | 6386179 |
| transitory recombination between plasmid phv33 and phage m13. | plasmid phv33 and phage m13 which have no homology exceeding 13 bp, combine in escherichia coli cells. the chimeric genome is encapsidated in phage proteins and injected into a recipient cell, where it decombines to regenerate the two parental genomes. we call this combination-decombination process 'transitory recombination'. | 1983 | 6365530 |
| in vitro deletional mutagenesis for bacterial production of the 20,000-dalton form of human pituitary growth hormone. | the 20,000-dalton (20k) variant form of human growth hormone (hgh) present in extracts from pituitary glands differs from the major form of hgh (22k, 191 amino acids) by the deletion of amino acid residues 32-46. using oligonucleotide-mediated mutagenesis, the dna coding for these amino acids was deleted from the gene previously constructed by us (goeddel et al., 1979) for microbial hgh production. the dna to be deleted was looped out by the annealing of a synthetic oligodeoxyribonucleotide to t ... | 1983 | 6357679 |
| the use of bacteriophage m13 carrying defined fragments of the escherichia coli glta gene to determine the location and structure of the citrate synthase promoter region. | the glta gene from escherichia coli, which encodes citrate synthase, has been located on a 3.24 kb hindiii/ecorl restriction fragment. this region contains one restriction site for bamhl and two for bglii. defined restriction fragments from this region were cloned into suitably cleaved replicative form m13mp8 and m13mp9. the recombinants (m13gtla1 leads to 10) were isolated as single stranded dna and characterised on the basis of molecular weight and dna sequence. the single stranded dna was con ... | 1983 | 6355771 |
| m13 procoat and a pre-immunoglobulin share processing specificity but use different membrane receptor mechanisms. | bacteriophage m13 procoat is accurately processed to transmembrane coat protein by salt-washed or n-ethylmaleimide-treated rough microsomes from dog pancreas. these treatments inhibit the processing of eukaryotic secreted protein precursors. m13 procoat can assemble into dog pancreas microsomes post-translationally. thus, the microsomal proteins needed for assembly may be determined by the nature of the precursor protein itself. these results, and our finding that the mouse igg kappa chain fragm ... | 1983 | 6344069 |
| interference between m13 and orim13 plasmids is mediated by a replication enhancer sequence near the viral strand origin. | the origin of replication for the viral strand of bacteriophage m13 dna is contained within a 507 base-pair intergenic region of the phage chromosome. the viral strand origin is defined as the specific site at which the m13 gene ii protein nicks the duplex replicative form of m13 dna to initiate rolling-circle synthesis of progeny viral dna. using in vitro techniques we have constructed deletion mutations in m13 dna at the unique avai site which is located 45 nucleotides away on the 3' side of t ... | 1984 | 6332917 |
| initiation and termination signals for transcription in bacteriophage m13. | transcription of the infrequently expressed phage m13 genome domain, comprising genes iii, vi, i and iv, has been studied in detail by hybridization and s1-nuclease mapping studies. the contiguous genes iii and vi are transcribed via an 1800 nucleotide-long rna molecule that is initiated at a promoter which overlaps with the rho-independent termination signal between genes iii and viii. its synthesis is terminated at a rho-dependent terminator in the proximal part of gene i. transcription of gen ... | 1984 | 6328409 |
| some extrachromosomal circular dnas containing the alu family of dispersed repetitive sequences may be reverse transcripts. | a 300 base-pair (bp) size class of small polydisperse circular dna (spcdna) isolated from the bsc-1 line of african green monkey kidney cells was cleaved with the restriction endonuclease sau3a, and the resulting fragments (100 to 200 bp) were cloned in bacteriophage m13 mp7. the nucleotide sequence of each of 24 clones containing dna sequences homologous to the alu family of mobile, dispersed, repetitive elements was then determined. analysis of these sequences revealed that many, and perhaps a ... | 1984 | 6325707 |
| screening recombinant phage m13 plaques with rna probes; a one-step procedure which identifies clones containing either of the complementary dna strands. | we describe a method for detecting specific dna sequences cloned in m13 phage vectors, based on the procedure of woo (in wu, r., methods in enzymology, vol. 68, academic press, new york, 1979, pp. 389-395). m13 plaques are adsorbed to a nitrocellulose filter that has been pre-saturated with bacteria. the filter is incubated on an agar plate to amplify the phage; the dna is alkali-denatured and then hybridized with a radioactive rna probe. unlike standard procedures, this method detects and disti ... | 1984 | 6325302 |
| in vitro generation of specific deletions in dna cloned in m13 vectors using synthetic oligodeoxyribonucleotides: mutants in the 5'-flanking region of the yeast alcohol dehydrogenase ii gene. | deletion mutants are particularly useful in defining the boundaries of noncoding genetic functions. such mutants can be precisely generated using synthetic oligodeoxyribonucleotides as mutagens. in this paper we describe the application of this method to recombinant dna cloned in a phage m13-derived vector. the mutagenic oligodeoxyribonucleotides, 20 and 21 nucleotides in length, were used to delete a tract of 20 da-dt base-pairs and an adjacent 22 base-pair perfect dyad from the adr3 locus, the ... | 1984 | 6324118 |
| use of m13mp phages to study gene regulation, structure and function: cloning and recombinational analysis of genes of the salmonella typhimurium histidine operon. | a restriction map was determined for a phi 80 lambda dhis transducing phage dna carrying the salmonella typhimurium histidine operon. dna fragments containing the promoter/regulatory region and the first two structural genes of the histidine operon (hisogd) were identified by their ability to direct regulated synthesis of histidinol dehydrogenase (product of hisd) in a coupled in vitro protein synthesizing system. a 3.1-kb sali-ecori restriction fragment containing the hisogd region, was subclon ... | 1983 | 6323256 |
| new versatile cloning and sequencing vectors based on bacteriophage m13. | a new pair of cloning and sequencing vectors based on bacteriophage m13mp7 has been developed. these vectors (m13tg130 and m13tg131) contain, in addition to the ecori, bamhi, hindiii, smai, sali and psti sites present in other vectors [cf., m13mp8 and m13mp9, messing and vieira, gene 19 (1982) 269-276], unique restriction recognition sequences for the enzymes ecorv, kpni, sphi, ssti and xbai. a restriction site for the enzyme bglii has been incorporated into the polylinker region of one of the v ... | 1983 | 6323254 |
| genes involved in transitory recombination between phage m13 and plasmid phv33. | plasmid phv33 and phage m13 combine in escherichia coli cells to form a chimera, which decombines to regenerate two parental genomes. combination can occur via two genetic pathways, one defined by the recbc genes, the other by reca, recf and possibly recl genes. decombination can also occur via two pathways, one defined again by the recbc genes, the other by a gene not identified, but active only in the absence of the recl gene product. | 1984 | 6323172 |
| replication functions of pc194 are necessary for efficient plasmid transduction by m13 phage. | escherichia coli plasmids pbr313 and pbr322 were transduced by phage m13 with low efficiency (10(-8) transductants/phage). hybrid plasmids phv12 or phv33, composed of staphylococcus aureus plasmid pc194 and pbr313 or pbr322, respectively, were transduced much more efficiently (10(-4) transductants/phage). inactivation of either of the two zones necessary for pc194 replication, one coding for a protein, the other not, reduced the transforming efficiency of hybrids to the level of pbr322. activity ... | 1984 | 6323171 |
| template requirements for the initiation of adenovirus dna replication. | the first step in the replication of the adenovirus genome is the covalent attachment of the 5'-terminal nucleotide, dcmp, to the virus-encoded terminal protein precursor (ptp). this reaction can be observed in vitro and has been previously shown to be dependent upon either viral dna or linearized plasmid dna containing viral terminal sequences. plasmids containing deletions or point mutations within the viral terminal sequence were constructed by site-directed mutagenesis. in the case of linear ... | 1984 | 6320160 |
| characterization of the dna binding protein encoded by the n-specific filamentous escherichia coli phage ike. binding properties of the protein and nucleotide sequence of the gene. | a dna binding protein encoded by the filamentous single-stranded dna phage ike has been isolated from ike-infected escherichia coli cells. fluorescence and in vitro binding studies have shown that the protein binds co-operatively and with a high specificity to single-stranded but not to double-stranded dna. from titration of the protein to poly(da) it has been calculated that approximately four bases of the dna are covered by one monomer of protein. these binding characteristics closely resemble ... | 1983 | 6312049 |
| expression of the human interferon-beta gene cloned in phage m13 mp7. | the single-stranded dna phage, m13 mp7 was used in the construction of an expression vector containing the coding sequence for mature interferon-beta (ifn-beta). two clones expressed a fused polypeptide showing the biological and physicochemical properties of ifn-beta, despite the fact that the n-terminal amino acid sequence had been changed; 10(6) i.u./l of culture were produced with a molecular weight of 20 000. | 1983 | 6311267 |
| kilo-sequencing: creation of an ordered nest of asymmetric deletions across a large target sequence carried on phage m13. | 1983 | 6310345 | |
| microinjected simian virus 40 crna is spliced, as evidenced by electron microscopy. | simian virus 40 crna was transcribed in vitro from the early viral dna strand. the rna was injected through glass capillaries into the nuclei of monkey cells. after a 2-h incubation, the rnas were extracted and hybridized to single-stranded simian virus 40 dna sequences contained in a bacteriophage m13 vector. electron microscopy revealed processed crnas with splice loops in the region of the intron of large t antigen. | 1983 | 6310149 |
| construction of a cloned library of adenovirus dna fragments in bacteriophage m13. | the construction of recombinant m13 phages containing adenovirus dna inserts was undertaken to provide strand-specific hybridization probes for analyses of adenovirus type 2 rna transcripts. a library of molecular probes was constructed by cloning restriction endonuclease fragments of adenovirus types 2 and 5 dna in the duplex replicative form dna of the single-stranded bacteriophage vectors, m13mp7, m13mp8, and m13mp9 (messing, j., and vieira, j. (1982) gene 19,269-276). adenovirus dna segments ... | 1983 | 6309766 |
| sandwich hybridization as a convenient method for the detection of nucleic acids in crude samples. | a method based on three-dna-component, sandwich hybridization has been designed for the detection and quantitation of nucleic acids in crude samples using adenovirus dna as a model. two non-overlapping restriction fragments of adenovirus type 2 (ad2) dna were cloned into two vectors, the pbr322 plasmid and m13 phage. the recombinant plasmid dna was immobilized onto nitrocellulose filters and the single-stranded recombinant phage dna was labeled with 125i and used as a probe. when these two reage ... | 1983 | 6301952 |
| nucleotide sequence of bacteriophage f1 dna. | the nucleotide sequence of the dna of the filamentous coliphage f1 has been determined. in agreement with earlier conclusions, the genome was found to comprise 6,407 nucleotides, 1 less than that of the related phage fd. phage f1 dna differs from that of phage m13 by 52 nucleotide changes, which lead to 5 amino acid substitutions in the corresponding proteins of the two phages, and from phage fd dna by 186 nucleotide changes (including the single-nucleotide deletion), which lead to 12 amino acid ... | 1982 | 6292494 |
| cloning and transcriptional control of a eucaryotic permease gene. | the uracil permease gene of the yeast saccharomyces cerevisiae was cloned on a hybrid plasmid which replicates autonomously in both yeast and escherichia coli. cloning was carried out by complementation in yeast. the smallest dna fragment found to complement the uracil permease deficiency in recipient yeast cells measured approximately 2.3 kilobases. in strains transformed by the plasmid with the uracil permease gene inserted, initial rates of uracil uptake increased up to 25 times more than the ... | 1982 | 6290876 |
| construction of gapped circular dna from phage m13 by in vitro hybridization. | 1982 | 6282331 | |
| sequencing long dna fragments cloned in bacteriophage m13 by using internal primers. the sequence analysis of a yeast dna fragment containing a replication origin. | in the ;shotgun' procedure for sequencing dna, dna fragments are cloned into a phage m13 vector and sequenced by using a flanking primer. in a variation of this procedure a longer dna sequence is cloned into m13, the two single-stranded recombinants identified and sequenced by using a set of internal primers prepared by exonuclease iii digestion of restriction fragments. | 1981 | 6280678 |
| oligonucleotide-directed mutagenesis of gene ix of bacteriophage m13. | the synthetic oligodeoxyribonucleotide pcgaaagactacac has been applied as a site-specific mutagen to introduce a t leads to g transversion mutation at nucleotide position 1223 of the m13 dna sequence. the in vitro-induced conversion of a tat codon into a tag at this position resulted in gene ix mutants with an amber mutant character thereby confirming that this reading frame defines a gene of an essential phage protein. the gene ix amber mutants obtained grew well on sui (ser) and suiii (tyr) su ... | 1982 | 6278437 |
| viable deletions of the m13 complementary strand origin. | the single-stranded dna of bacteriophage m13 is converted to a duplex replicative form by a mechanism involving rna-primed initiation at a single unique site on the viral dna. the dna sequence that specifies the rna primer is contained largely within one of two adjacent hairpin structures protected from dnase degradation by rna polymerase. we have used in vitro techniques to construct a series of m13 mutants having deletions in the region of the complementary strand origin. deletions of the dupl ... | 1981 | 6273888 |
| the atp operon: nucleotide sequence of the region encoding the alpha-subunit of escherichia coli atp-synthase. | part of the atp (or unc) operon encoding the alpha, beta, gamma, delta, and epsilon subunits of escherichia coli atp-synthase has been cloned into the plasmid pacyc 184. the dna coding for the largest of these proteins, the alphas subunit, has been sequenced by cloning into the bacteriophage m13 and sequencing with dideoxy nucleotide chain terminators. it comprises 1539 nucleotides corresponding to a protein of 513 amino acids. | 1981 | 6272228 |
| [substrate specificity of ca2+,mg2+-dependent dnaase from sea urchin (strongylocentrotus intermedius) embryos]. | ca2+,mg2+-dependent dnase from sea urchin embryos is specific to the secondary structure of substrates irrespective of the nature of activating cations. the enzyme does not split synthetic single-stranded oligo and polynucleotides, such as d(ptptptpcpc), d(pgpgptptpt). d(papaptptpc), d(pgpapaptptpc), d(pa)5-poly(dt), d(papaptptpc)-poly(dt), poly(da) and poly (dt) and hydrolyses the double-stranded substrates poly d(at), poly (da) . poly (dt) and highly polymerized dna. native double-stranded dna ... | 1981 | 6271260 |
| the dna sequences of cloned complex satellite dnas from hawaiian drosophila and their bearing on satellite dna sequence conservation. | a class of restriction endonuclease fragments near 185 bp in length and comprising approximately 20% of the genomes of 3 species of hawaiian drosophila has been cloned using bacteriophage m13. the nucleotide sequences of 14 clones have been determined and the variation between clones has been found to be due to deletions and base changes. analyses of uncloned material show that the cloning system itself does not introduce the variation. the variation of the basic repeat within and between specie ... | 1981 | 6262029 |
| construction and characterization of new coliphage m13 cloning vectors. | new single-stranded dna cloning vectors have been constructed by the insertion of additional dna fragments into a haeii restriction site in the bacteriophage m13 duplex replicative form (rf). these inserts into the m13 genome bring a single restriction sites useful for cloning, including psti, xorii, ecori, ssti, xhoi, kpni, and pvuii. drug-resistance genes cloned into m13 include the beta-lactamase (bla) gene and the chloramphenicol acetyl transferase (cat) gene. these vectors provide a conveni ... | 1980 | 6260570 |
| nucleotide sequence of the filamentous bacteriophage m13 dna genome: comparison with phage fd. | the 6407 nucleotide-long sequence of bacteriophage m13 dna has been determined using both the chemical degradation and chain-termination methods of dna sequencing. this sequence has been compared with that of the closely related bacteriophage fd (beck et al., 1978). m13 dna appears to be only a single nucleotide shorter than fd dna. there is an average of 3.0% of nucleotide-sequence differences between the two genomes, but the distribution of these changes is not random; the sequence of some gen ... | 1980 | 6254849 |
| template function of restriction enzyme fragments of phage m13 replicative form dna. | 1980 | 6246375 | |
| replication of bacteriophage m13. xv. location of the specific nick in m13 replicative form ii accumulated in escherichia coli polaex1. | m13 replicative form ii (rfii) dna was prepared from escherichia coli rs5052 (polaex1) cells in the late stage of infection, and the dna sequence at the discontinuity was examined. the data presented here suggest that the single discontinuity in the late stage of infection rfii maps at the same position as the gene ii protein nicking site on fd rfi which was determined in vitro (meyer et al., nature (london) 278:365-367, 1979) and has a 5' terminal nucleotide sequence identical to that at the ni ... | 1980 | 6246252 |
| the nucleotide sequence of the akv murine leukemia virus genome. | the nucleotide sequence of an infectious molecular clone of the akv murine leukemia virus has been determined by the dideoxy chain termination method after subcloning in bacteriophage m13 vectors. the sequence predicts an rna genome of 8371 nucleotides containing three large open reading frames corresponding to the gag, pol, and env genes. signal sequences for transcription, splicing, and translation have been identified. the positions of 95 major rnase t1 resistant oligonucleotides of the akv r ... | 1984 | 6200992 |
| rapid purification of bacterial plasmids and coliphage m13 rf without cscl centrifugation. | 1982 | 6187239 | |
| decay of mrna in escherichia coli: investigation of the fate of specific segments of transcripts. | an assay was developed to investigate the fate of specific segments of beta-lactamase (bla) and ompa gene transcripts in escherichia coli. dna probes cloned in bacteriophage m13 were treated with an endonuclease capable of cleaving single-stranded dna, the fragments produced were annealed with total cellular rna, and the resulting rna . dna hybrids were subjected to s1 nuclease treatment and gel fractionation. by using this assay, direct evidence was obtained for 3'-to-5' directionality in the d ... | 1983 | 6187001 |
| use of a rapid dna sequencing system to demonstrate the induction of frameshift mutations by bleomycin. | the rapid dna sequencing system based on the single-stranded bacteriophage m13 and the chain-terminator method has been used to look directly for mutational alterations. a small dna fragment that primes dna synthesis through the n-terminal 200 base pairs of the beta-galactosidase gene was prepared, and used to detect changes in base sequence among phages that give white plaques after treatment of the host cells with bleomycin. bleomycin treatment of e. coli in which m13 mp2 was growing gave an i ... | 1982 | 6183148 |
| carboxy terminus of polyoma middle-sized tumor antigen is required for attachment to membranes, associated protein kinase activities, and cell transformation. | we have constructed a transformation-defective polyoma virus mutant (py 1387-t) that directs the synthesis of a normal small tumor antigen, a functional large tumor antigen, and a truncated (51,000-dalton) middle-sized tumor (mt) antigen that lacks 37 amino acids at its cooh terminus. the shortened mt polypeptide is missing the hydrophobic "tail" thought to be responsible for the anchorage of this protein into the plasma membrane and is in fact in cytosol fractions. this truncated mt polypeptide ... | 1982 | 6179082 |
| minimal size plasmids containing an m13 origin for production of single-strand transducing particles. | we have studied the requirements for efficient production of single-strand transducing particles from minimal size plasmids containing the phage m13 origin of replication. the most favorable origin fragment for production of transducing particles was found to extend from nucleotides 5372 to 5943 of the phage sequence, which includes a segment of the phage gene iv coding region and eliminates part of the gene ii promoter. minimizing vector size for m13 origin plasmids appears to be beneficial sin ... | 1984 | 6099398 |
| the gapped duplex dna approach to oligonucleotide-directed mutation construction. | a simple and efficient method is described to introduce structurally pre-determined mutations into recombinant genomes of filamentous phage m13. the method rests on gapped duplex dna (gddna) molecules of the phage m13 genome as the key intermediate. in this gddna, the (+) and the (shorter) (-) strand carry different genetic markers in such a way, that a rigorous selection can be applied for phage carrying the markers of the (-) strand. for introduction of the mutation, a synthetic oligonucleotid ... | 1984 | 6096830 |
| integration of a temperate phage infecting spiroplasma citri. | a physical map of the genome of a temperate type 3 spiroplasma-virus, ai, has been constructed. host dna has been digested with restriction enzymes, and recombinant dna clones of ai fragments in coliphage m13 vectors have been used as probes to detect viral dna sequences integrated into spiroplasmas. all strains of spiroplasma citri examined contained a deleted form of ai integrated as a cryptic prophage which was unable to confer resistance to ai superinfection. stable ai lysogens also containe ... | 1984 | 6096304 |
| high-sensitivity s1 mapping with single-stranded [32p]dna probes synthesized from bacteriophage m13mp templates. | a method is described by which high-specific-activity single-stranded (ss) [alpha-32p]dna of a defined size complementary to sequences cloned into bacteriophage m13 is synthesized. the ss dna template is annealed with a universal sequencing primer, the primer extended with dna polymerase i klenow fragment and the dna duplex cut at a unique site 5' to the multiple cloning sites in the m13 phage. the reaction products are denatured and the ss alpha-32p probe fragment complementary to the cloned se ... | 1984 | 6096224 |
| use of short dna oligonucleotides for determination of dna sequence modifications induced by benzo[a]pyrene diol epoxide. | various organic agents that alkylate dna are known to induce mutations in bacterial and animal cells. the precise nature and location of modified dna sequences in such mutants are often difficult to ascertain. in this report, a 10-base-pair oligomer (bamhi linker) is treated with (+/-)-trans-benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide and inserted into replicative form dna of phage m13 by ligation at a specific restriction site. escherichia coli are transfected with the recombinant dna containin ... | 1984 | 6091121 |
| ribonucleoprotein organization of eukaryotic rna. xxxi. structure of the u1 small nuclear ribonucleoprotein. | a small nuclear ribonucleoprotein, u1 snrnp, has been implicated in mrna processing. in this investigation sites of protein binding on u1 rna were mapped by nuclease protection and rna sequencing. partially purified human u1 snrnp was sequentially digested with escherichia coli rnaase iii and s1 nuclease. the resistant ribonucleoprotein fragments were deproteinized, preparatively hybridized to the u1 rna--complementary dna strand of a human u1 gene cloned in bacteriophage m13, and displayed by e ... | 1984 | 6084724 |
| conditional lethal mutants of the small filamentous coliphage m13. i. isolation, complementation, cell killing, time of cistron action. | 1966 | 5921643 | |
| conditional lethal mutants of the small filamentous coliphage m13. ii. two genes for coat proteins. | 1969 | 5807970 | |
| replication of the small coliphage m13: evidence for long-living m13 specific messenger rna. | 1970 | 5422625 | |
| replication of bacteriophage m13. 3. identification of the intracellular single-straned dna. | 1969 | 5401239 | |
| replication of bacteriophage m13. ii. the role of replicative forms in single-strand synthesis. | 1969 | 5401238 | |
| enzymatic mechanisms of dna replication. | dna polymerases purified from several sources are characterized by replication of the 3'-hydroxy-terminated strand of a helical template. failure to achieve simultaneous replication of the 5'-strand leads to aberrations in the synthesized dna, described as nondenaturability and branching. aberrations in synthesized dna were not observed when (a) the 5'-strand was destroyed by a specific nuclease during the course of replication or (b) a single-stranded (circular) phage (m13) dna served as templa ... | 1966 | 5338562 |
| purification and properties of diploid particles of coliphage m13. | 1967 | 5337710 | |
| the proteins of bacteriophage m13. | particles of the small filamentous coliphage m13 contain not only the major coat protein, which is the product of phage gene 8, but also a minor coat protein, the a protein, which is the product of gene 3. the a protein has a molecular weight of approximately 70,000 daltons, is present in one copy per virion, and is responsible for phage attachment to host cells. also associated with purified m13 particles is a minor quantity of very small proteinaceous material, but its origin as a phage-coded ... | 1969 | 5257006 |
| ultraviolet-induced cross-links in the deoxyribonucleic acid of single-stranded deoxyribonucleic acid viruses as a probe of deoxyribonucleic acid packaging. | deoxyribonucleic acid (dna) from ultraviolet (uv)-irradiated phix174 sediments in alkali at rates up to 1.7 times that of unirradiated phix174 dna and is observed as a condensed, cross-linked structure when examined in the electron microscope by the formamide spreading technique. this structure appears to result from multiple cross-links induced in the tightly coiled dna contained within the spherical phix174 capsid. in contrast, the dna extracted after uv irradiation of the filamentous bacterio ... | 1972 | 5036669 |
| role of coliphage m13 gene 5 in single-stranded dna production. | 1971 | 4943754 | |
| replication of bacteriophage m13. detachment of the parental dna from the host membrane and transfer to progeny phages. | 1971 | 4942146 | |
| a possible role for rna polymerase in the initiation of m13 dna synthesis. | the conversion of single-stranded dna of bacteriophage m13 to the double-stranded replicative form in escherichia coli is blocked by rifampicin, an antibiotic that specifically inhibits the host-cell rna polymerase. chloramphenicol, an inhibitor of protein synthesis, does not block this conversion. the next stage in phage dna replication, multiplication of the doublestranded forms, is also inhibited by rifampicin; chloramphenicol, although inhibitory, has a much smaller effect. an e. coli mutant ... | 1971 | 4941987 |
| replication of bacteriophage m13. vi. attachment of m13 dna to a fast-sedimenting host cell component. | 1971 | 4940969 | |
| genetic control of bacteriophage m13 dna synthesis. | 1968 | 4939035 | |
| non-essentiality of the reca- mutation in the phenomenon of bacteriophage m13-induced elimination of f' factors. | the elimination of f' factors promoted by coliphage m13 infection can occur in reca(+) as well as reca(-) merodiploid strains of escherichia coli k-12. | 1971 | 4934058 |
| replication of bacteriophage m13. v. single-strand synthesis during m13 infection. | 1971 | 4930572 | |
| membrane attachment of replicating parental dna molecules of bacteriophage m13. | 1971 | 4927799 | |
| replication of bacteriophage m13. iv. synthesis of m13-specific dna in the presence of chloramphenicol. | 1970 | 4923999 | |
| conformations of the single-stranded dna of bacteriophage m13. | at least two conformations of m13 single-stranded dna have been demonstrated by measuring differences in sedimentation coefficient and by direct visualization in the electron microscope. which form is obtained from infected cells and/or intact phage depends on the ph, ionic strength, and temperature. the slower-sedimenting form can be converted to the faster-sedimenting, single-stranded form by low ionic strength, alkali treatment, formamide, or formaldehyde, but not by exposure to 100 degrees c ... | 1970 | 4922292 |
| increased fragility of escherichia coli after infection with bacteriophage m13. | male strains of escherichia coli infected with filamentous phage m13 released the progeny phage particles from intact cells. at the same time, the cells continued to grow and multiply at a slightly lower rate than the uninfected cells. concomitant with the phage release, lipopolysaccharide from the cell wall of the infected cells was also released. the buoyant density of e. coli hfrc in diaginol, 1.25 g/cc, did not change as a result of infection. detergents like sodium dodecyl sulfate and sarko ... | 1970 | 4921123 |
| susceptibility of e. coli k-12 to actinomycin d after infection with phage m13. | 1970 | 4919773 | |
| interference by bacteriophage t4 in the reproduction of the single-stranded dna phage m13. | 1970 | 4918275 | |
| replication of the single-stranded dna bacteriophage m13: absence of intracellular phages. | 1970 | 4915300 | |
| loss of an episomal fertility factor following the multiplication of coliphage m13. | 1969 | 4904513 | |
| replication of the single-stranded dna bacteriophage m13: messenger rna synthesis directed by m13 replicative form dna. | 1969 | 4902522 | |
| replication of bacteriophage m13. i. sedimentation analysis of crude lysates of m13-infected bacteria. | 1969 | 4890599 | |
| replication of bacteriophage m13. 8. differential effects of rifampicin and nalidixic acid on the synthesis of the two strands of m13 duplex dna. | 1974 | 4817800 | |
| role of bacteriophage m13 gene 2 in viral dna replication. | 1972 | 4648116 | |
| replicative intermediates in bacteriophage m13 single stranded dna synthesis. | 1974 | 4611894 | |
| binding, eclipse, and penetration of the filamentous bacteriophage m13 in intact and disrupted cells. | 1974 | 4608378 | |
| complex of bacteriophage m13 single-stranded dna and gene 5 protein. | 1974 | 4594145 | |
| loss of r factors promoted by bacteriophage m13 and the m13 carrier state. | the infection of different hfr strains of escherichia coli bearing derepressed r factors of the fi(+) or fi(-) type can result in the loss of the r factor and the conversion of the infected cells to the r(-) state. this extends earlier observations on the elimination of f' factors by bacteriophage m13 infection. variability in the efficiency of this conversion can arise because of genetic factors independent of the r factor being eliminated. a fraction of the infected but unconverted r(+) cells ... | 1972 | 4591485 |
| genetic transfer of episomic elements among erwinia species and other enterobacteria: f'lac+. | the episomic element f'lac(+) was transferred, probably by conjugation, from escherichia coli to lac(-) strains of erwinia herbicola, erwinia amylovora, and erwinia chrysanthemi (but not to several other erwinia spp. in preliminary trials). the lac genes in the exconjugants of the erwinia spp. showed varying degrees of stability depending on the strain (stable in e. herbicola strains y46 and y74 and e. amylovora strain ea178, but markedly unstable in e. chrysanthemi strain ec16). the lac genes a ... | 1972 | 4591473 |
| antiserum inactivation of electrophoretically purified m13 diploid virions: model for the f-specific filamentous bacteriophages. | antiserum inactivation experiments were carried out on electrophoretically purified diploid virions from a cross between two complementing amber mutants of phage m13. the total (homozygous plus heterozygous) diploid population, assayed on a permissive host where only one genome is needed for plaque formation, was inactivated at the same rate as haploids. heterozygous diploids, assayed on a nonpermissive host, where both genomes are needed for plaque formation, were twice as sensitive as haploids ... | 1974 | 4589858 |
| synthesis of bacteriophage m13-specific proteins in a dna-dependent cell-free system. ii. in vitro synthesis of biologically active gene 5 protein. | it is shown that gene 5 protein of bacteriophage m13 is one of the major proteins synthesized in vitro under the direction of m13 replicative-form dna. by means of dna-cellulose chromatography, this protein has been purified to homogeneity and its biological characteristics have been compared with those of its native counterpart. like native gene 5 protein, the purified, in vitro-synthesized protein binds tightly and selectively to single-stranded, but not to double-stranded, dnas. these results ... | 1973 | 4586780 |