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temporal expression of hepatic estrogen receptor 1, vitellogenin1 and vitellogenin2 in european silver eels.because european silver eels have never been caught during or after their 6000-km reproductive migration to the sargasso sea, all existing knowledge on their sexual maturation comes from hormonal stimulation. silver eels that start their oceanic migration are still immature with pre-vitellogenic oocytes. hence we assumed that vitellogenesis should start with the expression of the estrogen receptor in the liver before the circulating 17beta-estradiol (e2) can have any effect. in this study we fol ...201019766647
phospholamban s-nitrosylation modulates starling response in fish heart.the frank-starling mechanism is a fundamental property of the vertebrate heart, which allows the myocardium to respond to increased filling pressure with a more vigorous contraction of its lengthened fibres. in mammals, myocardial stretch increases cardiac nitric oxide (no) release from both vascular endothelium and cardiomyocytes. this facilitates myocardial relaxation and ventricular diastolic distensibility, thus influencing the frank-starling mechanism. in the in vitro working heart of the e ...200919726482
molecular and physiological study of the artificial maturation process in european eel males: from brain to testis.european eel males can be artificially matured (1.5iu hcg/g fish), but the regulatory mechanisms of their reproductive development are practically unknown. spermatogenic stages (s1-s6), biometric characters [eye index (ei), gonadosomatic index (gsi), hepatosomatic index (hsi)] and sperm quality parameters (motility, viability and head spermatozoa morphometry) were analysed. moreover, the present study evaluated the expression of gnrhs (mammal and chicken ii gonadotropin release hormone i) and go ...201019699741
expression and functional characterization of four aquaporin water channels from the european eel (anguilla anguilla).the european eel is a euryhaline teleost which has been shown to differentially up- and downregulate aquaporin (aqp) water channels in response to changes in environmental salinity. we have characterized the transport properties of four aquaporins localized to osmoregulatory organs - gill, esophagus, intestine and kidney. by sequence comparison these four aqp orthologs resemble human aqp1 (eel aqp1), aqp3 (eel aqp3) and aqp10 (aqpe). the fourth member is a duplicate form of aqp1 (aqp1dup) though ...200919684221
sex-related differences in aerobic capacities and reactive oxygen species metabolism in the silver eel.silver european eels (anguilla anguilla l.) need to develop important aerobic capacities to cope with their long fasting spawning migration at depth, particularly males which are about half the size of females. moreover, they have to face potential oxidative stress because reactive oxygen species (ros) production is linked to the increase in metabolic rate. thus, aerobic metabolism was globally evaluated in male and female silver eels exposed to a 10.1 mpa hydrostatic pressure (1,000 m depth). o ...201019680762
anthropogenic impacts on the orbetello lagoon ecosystem.the aim of the present study was to assess the environmental quality of orbetello lagoon (tyrrhenian coast, italy), using a biomonitoring method based on measuring organochlorinated pollutants in the anguilla anguilla, chosen as sentinel organism, and remote sensing data obtained by elaboration of landsat 7 enhanced thematic mapper (etm) data of 2002. polychlorinated biphenyls and organochlorine pesticides were determined by gas chromatography/mass spectrometry in muscle of european eels capture ...200919651809
abundant expression and purification of biologically active mitochondrial citrate carrier in baculovirus-infected insect cells.heterologous expression of recombinant proteins is an essential technology for protein characterization. a major obstacle to investigating the biochemical properties of membrane proteins is the difficulty in obtaining sufficient amounts of functional protein. here we report the successful expression of the tricarboxylate (or citrate) carrier (cic) of eel (anguilla anguilla) from spodoptera frugiperda (sf9) cells using the baculovirus expression system. the recombinant cic was purified by affinit ...200919629661
transcript profiling and dna damage in the european eel (anguilla anguilla l.) exposed to 7,12-dimethylbenz[a]anthracene.the molecular responses induced during and after an acute exposure to 7,12-dimethylbenz[a]anthracene (dmba) were analysed in liver, gill and blood cells of juvenile anguilla anguilla with the aim of developing molecular biomarkers of environmental pah pollution. changes in the mrna expression levels of the cell cycle checkpoint-related rad1 gene and the mrnas of differentially expressed genes by suppression subtractive hybridization (ssh) were analysed in the liver, and related to well-establish ...200919586669
brain derived neurotrophic factor and trk b mrna expression in the brain of a brain stem-spinal cord regenerating model, the european eel, after spinal cord injury.evidence from mammalian studies suggests that brain derived neurotrophic factor (bdnf) and its receptor, trk b, are upregulated in neuronal cell bodies after injury. although fish possess neurotrophins and display rapid functional and morphological recovery after central nervous system (cns) injury, to date few studies have examined neurotrophin expression during cns regeneration. in this study, rt-pcr was used to investigate the effect of complete spinal cord transection on the mrna expression ...200919545610
improvement of european eel sperm cryopreservation method by preventing spermatozoa movement activation caused by cryoprotectants.sperm production has been obtained from european and japanese eels, but its quality and quantity tend to be changeable. so, its cryopreservation has been tried in both species. dimethyl sulfoxide (me(2)so) is the best cryoprotectant for european eel sperm, but increases the medium osmolality, inducing the activation of spermatozoa motility. to avoid this, different combinations of ph (6.5 and 8.5) and nahco(3) concentrations (20, 40 and 80mm) were tested with two me(2)so concentrations (5% and 1 ...200919508865
ovarian gene transcription and effect of cadmium pre-exposure during artificial sexual maturation of the european eel (anguilla anguilla).european eels are dangerously threatened with extinction. recent advances tend to show that pollution could, in addition to other already identified factors, contribute to this drama. in a previous report, cadmium (cd) pre-exposure was found to strongly stimulate the pituitary-liver-gonad axis of maturing female silver eels, leading, lastly, to oocytes atresia and eels mortality. the present work was performed to get more insights into the effects of cd preexposure on eels' ovaries. the transcri ...200919484408
monitoring of herpesvirus anguillae (hva) infections in european eel, anguilla anguilla (l.), in northern germany. 200919460086
regulation of expression of the myo-inositol monophosphatase 1 gene in osmoregulatory tissues of the european eel anguilla anguilla after seawater acclimation.previous microarray studies in our laboratory identified a number of genes that were differentially expressed in "silver" eels after transfer from freshwater (fw) to seawater (sw). a group of genes, which are related to the synthesis, processing, and transport of certain known osmolytes in mammalian cells, have been identified. one gene implicated with osmolyte production is myo-inositol monophosphatase (impa1). the aim of this study was to compare the expression of impa1 in the major osmoregula ...200919456379
panmixia in european eel revisited: no genetic difference between maturing adults from southern and northern europe.previous studies of genetic structure in the european eel have resulted in seemingly conflicting results, ranging from no detectable heterogeneity to small but statistically significant differences and isolation by distance patterns among eels sampled across the continental range. differences with respect to sampling design and choice of molecular markers, combined with a lack of power estimates, complicate comparisons of existing results. in this study we have used six microsatellite markers an ...200919417764
pcbs and the energy cost of migration in the european eel (anguilla anguilla l.).the effect of polychlorinated biphenyls (pcbs) on the energy consumption of fasting silver european eel (anguilla anguilla l.) was studied over a 27-day period during which the animals were at rest or were swimming 800 km in blazka swim tunnels. three-year-old female hatchery eels (silver stage) between 73 and 80 cm long weighing around 1 kg were dosed intraperitoneally with pcbs at a nominal dosage of 10x the consumption standard as a mixture representative for planar (7 microg pcb126/kg eel), ...200919371957
nitrite modulates contractility of teleost (anguilla anguilla and chionodraco hamatus, i.e. the antarctic hemoglobinless icefish) and frog (rana esculenta) hearts.being the largest form of intravascular and tissue storage of nitric oxide (no) and a signalling molecule itself, the nitrite anion (no(2)(-)) has emerged as a key player in many biological processes. since the heart is under an important no-mediated autocrine-paracrine control, in mammals the cardiac effects of nitrite are under intensive investigation. in contrast, nothing is known in non-mammalian vertebrates. we evaluated nitrite influence on cardiac performance in the perfused beating heart ...200919298789
brain-derived neurotrophic factor mrna expression in the brain of the teleost fish, anguilla anguilla, the european eel.the spatial expression pattern of brain-derived neurotrophic factor (bdnf) mrna in the brain of the european eel (anguilla anguilla) was determined using non-radioactive in situ hybridization, and mapped and compared to that of other vertebrates. riboprobes were prepared based on a partial cdna coding sequence for a. anguilla bdnf that was amplified using degenerate primers, cloned and sequenced. as in other animal groups, in the eel, bdnf mrna expression was seen in the telencephalon, hypothala ...200919246895
experimental infection and detection of aphanomyces invadans in european catfish, rainbow trout and european eel.european catfish silurus glanis, european eel anguilla anguilla and rainbow trout oncorhynchus mykiss were challenged by intramuscular injection of zoospores of aphanomyces invadans, the oomycete associated with epizootic ulcerative syndrome (eus). the tropical three-spot gourami trichogaster trichopterus is known to be highly susceptible and was used as a positive control. european catfish were highly susceptible and rainbow trout had moderate to low susceptibility, whereas eels appeared largel ...200819244971
cortisol mobilizes mineral stores from vertebral skeleton in the european eel: an ancestral origin for glucocorticoid-induced osteoporosis?endogenous excess cortisol and glucocorticoid (gc) therapy are a major cause of secondary osteoporosis in humans. intense bone resorption can also be observed in other vertebrates such as migratory teleost fish at the time of reproductive migration and during fasting when large amounts of calcium and phosphate are required. using a primitive teleost, the european eel, as a model, we investigated whether cortisol could play an ancestral role in the induction of vertebral skeleton demineralization ...200919223398
f-type lectin from the sea bass (dicentrarchus labrax): purification, cdna cloning, tissue expression and localization, and opsonic activity.recently described biochemical and structural aspects of fucose-binding lectins from the european eel (anguilla anguilla) and striped bass (morone saxatilis) led to the identification of a novel lectin family ("f-type" lectins) characterized by a unique sequence motif and a characteristic structural fold. the f-type fold is shared not only with other members of this lectin family, but also with apparently unrelated proteins ranging from prokaryotes to vertebrates. here we describe the purificati ...200919162197
yellow eel (anguilla anguilla) development in nw portuguese estuaries with different contamination levels.the aims of the present study were to compare the health status of yellow eels (anguilla anguilla) developing in three estuaries of the nw portuguese coast with different levels of pollution and their physiological responses to combined effects of environmental variation and pollution. for this, a field study was performed using a multi-parameter approach, including eels condition indexes and biomarkers, water quality variables and other environmental factors. sixteen biological parameters were ...200919123036
physiological responses to starvation in the european eel (anguilla anguilla): effects on haematological, biochemical, non-specific immune parameters and skin structures.the physiological effects of short-term starvation on some haematological, biochemical and non-specific immune response parameters together with the histological structure of the skin, were investigated in the european eel, anguilla anguilla. blood haemoglobin and haematocrit, serum glucose and cortisol, hemolysins, haemagglutinins, and lysozyme in the plasma, kidney and epidermal extract, were measured in fish after 31, 42 and 58 days of starvation, and compared to those of fed fish. starvation ...201019093221
a preliminary observation on the pond culture of european eel, anguilla anguilla (linnaeus, 1758) in egypt: recommendations for future studies.an experiment was conducted to assess the potential of the european eel, anguilla anguilla for earthen pond aquaculture without supplementary feeding at lake manzala, egypt. juvenile a. anguilla of mean length 11.7 cm and 2.4 g weight were stocked in earthen ponds measuring 3 feddans (about 12,600 m2) and 1 m deep. stocking was done in may 2003 at a rate of 5000 fish feddan(-1) in a polyculture system including tilapia and mullets and fed mainly on natural occurring prey (natural spawned tilapia ...200719070049
guanylin-like peptides, guanylate cyclase and osmoregulation in the european eel (anguilla anguilla).three guanylin-like peptides, guanylin, uroguanylin and renoguanylin and two guanylate cyclase type c (gc-c) receptor isoforms were cloned and sequenced from the european eel (anguilla anguilla). all peptides and both receptors (gc-c1 and gc-c2) were predominantly expressed within the intestine and kidney of both sexually immature yellow, and sexually maturing, migratory silver eels. the derived amino acid sequences for the pre-prohormones and guanylate cyclase isoforms had structural features i ...200919028495
molecular cloning and characterization analysis of immunoglobulin m heavy chain gene in european eel (anguilla anguilla).in this study, the immunoglobulin m heavy chain gene of european eel (anguilla anguilla) was cloned and analyzed. the full-length cdna of the igm heavy chain gene (genbank accession no. ef062515) has 2089 nucleotides encoding a putative protein of 581 amino acids. the igm heavy chain was composed of leader peptide (l), variable domain (vh), ch1, ch2, hinge, ch3, ch4, and c-terminus and two novel continuous putative n-glycosylation sites were found close to the second cysteine of ch3 in a. anguil ...200919013650
trace element concentrations in proteocephalus macrocephalus (cestoda) and anguillicola crassus (nematoda) in comparison to their fish host, anguilla anguilla in ria de aveiro, portugal.the use of some fish parasites as bioindicators of heavy metal pollution has been demonstrated as particularly adequate due to their capacity of bioconcentration. this study evaluated the effect of proteocephalus macrocephalus on the accumulation of trace elements in the edible fish, anguilla anguilla, in a contaminated area in portugal (ria de aveiro). also, the model p. macrocephalus/a. anguilla was assessed as a bioindicator system in the presence of the highly prevalent nematode anguillicola ...200919010518
two distinct dopamine d2 receptor genes in the european eel: molecular characterization, tissue-specific transcription, and regulation by sex steroids.two full-length cdna encoding putative dopamine d2-like receptors were cloned from the brain of female european eel. the deduced protein sequences, termed d2a- and d2b-r, exhibit closer phylogenetic relationships to vertebrate d2 receptors compared with d3 and d4 or d1 receptors. the two protein sequences share 100% identity within the transmembrane domains containing the highly conserved amino acids involved in dopamine binding. accordingly, an apparent single population of sites on eel brain m ...200918974275
effects of propanil on the european eel anguilla anguilla and post-exposure recovery using selected biomarkers as effect criteria.the aim of this study was to assess the physiological response of anguilla anguilla to propanil and the degree of recovery after being moved to clean water. preliminary acute toxicity test was carried out in the laboratory and the median lethal concentration (lc50) at 96 h was calculated as 31.33 mg/l (29.60-33.59 mg/l). noec and loec values (at 96 h) were also calculated as 20 and 25mg/l, respectively. the fish were exposed to 0.63 and 3.16 mg/l of propanil for 72 h and allowed to recover for 1 ...200918973941
european eel sperm diluent for short-term storage.the sperm of european eel shows a high density and the time of spermatozoa motility is very short after activation with sea water. these characteristics make difficult the sperm handling and its quality assessment. several diluents were previously described for the japanese eel obtaining over 3 weeks' conservation times under refrigeration, but they rendered bad results in the european species. in the present study, several diluents were developed taking as basis the p1 medium, and using differe ...201018954399
regulation of anguillicola crassus (nematoda) infrapopulations in their definitive host, the european eel, anguilla anguilla.the parasitic nematode anguillicola crassus was recently introduced into populations of the european eel, anguilla anguilla. we investigated, under experimental conditions, the regulation of a. crassus infrapopulations. we tested the effects of (1) the resource-limited habitat of the parasite and (2) the coexistence of several developmental stages in its niche (the swim-bladder) on the composition of the infrapopulations. the results revealed that the respective effects of these factors differed ...200818940021
the inflammatory response of fish to helminth parasites.fish serve as a good model for studying vertebrate immune systems because they have a relatively simple system. descriptions of histopathological effects of helminth parasites on fish are few and far between with limited observations made on the identification of the inflammatory cells involved in the host reaction. recently, two cell types found within teleosts received a great deal of attention, namely mast cells and rodlet cells. fish most cells also known as eosinophilic granule cells, are m ...200818814717
glutathione transferases from anguilla anguilla liver: identification, cloning and functional characterization.glutathione transferases (gsts) constitute a class of detoxifying enzymes involved in phase ii metabolism. using gsh-affinity chromatografy followed by hplc analysis, two gst isoforms were isolated from the anguilla anguilla liver cytosol. the major gst belongs to the piscine-specific rho class and accounted for about 59% of total gst affinity eluted fraction, while the remaining 41% was represented by a pi class gst. both isoforms were cloned, heterologously expressed in escherichia coli and th ...200818804293
glycolytic fluxes in european silver eel, anguilla anguilla: sex differences and temperature sensitivity.european silver eels migrate 6000 km to their supposed spawning area in the sargasso sea. as the eel is fasting, this intense swimming activity is realised only with fat stores, involving mainly red muscle i.e. aerobic metabolism. however, eel migration is performed at depth and thus in cold water, both being known to induce changes in muscle energy metabolism. during migration, white and red muscles can operate together or separately in order to counteract the eventual effects of low temperatur ...200818789394
population structure of the parasitic nematode anguillicola crassus, an invader of declining north atlantic eel stocks.probably half of all animal species exhibit a parasitic lifestyle and numerous parasites have recently expanded their distribution and host ranges due to anthropogenic activities. here, we report on the population genetic structure of the invasive nematode anguillicola crassus, a parasite in freshwater eels, which recently spread from asia to europe and north america. samples were collected from the newly colonized naive host species anguilla anguilla (europe) and anguilla rostrata (north americ ...200818727770
ammonia sensitivity of the glass eel (anguilla anguilla l.): salinity dependence and the role of branchial sodium/potassium adenosine triphosphatase.eel aquaculture is capture based and thus dependent on the fishery for juvenile glass eels. this fishery typically takes place in estuaries where salinity varies and ammonia levels can be elevated. also, during capture and transport glass eels are kept at high densities and ammonia from endogenous production can increase to toxic levels. ammonia is known to have detrimental effects on fish growth and survival. in the present study, the salinity dependence of ammonia sensitivity in glass eels acc ...200918717620
the suitability of cytochrome-p4501a1 as a biomarker for pcb contamination in european eel (anguilla anguilla).european eel (anguilla anguilla) elvers were intraperitoneally injected with different doses of 3,3',4,4'-tetrachlorobiyphenyl (pcb77) to examine and characterize the inductive effect of coplanar pcbs on cyp1a1 gene expression in liver and gills by using a semi-quantitative rt-pcr analysis. the influence of pcb77 injection on transcription activity of the housekeeping gene glyceraldehyde-3-phosphate-dehydrogenase (gapdh) was tested to determine its suitability as a reference gene for further qua ...200818657580
demonstration of bone resorbing cells in elasmobranchs: comparison with osteoclasts.we have previously shown that elasmobranchs-characterized by a partially calcified cartilaginous endoskeleton-presented a bony vertebral arch containing osteoblasts, osteocytes and resorbing cells. the aim of this study is to test the ability of elasmobranchs to resorb bone tissue. the subcutaneous implantation in dogfish (scyliorhinus canicula) of devitalized mineral-containing bone particles, obtained from a bony fish (the eel, anguilla anguilla) resulted, after 21 2 months, in the formation o ...198918620290
gossypol affects ion transport in the isolated intestine of the seawater adapted eel, anguilla anguilla.cottonseed (gossypium sp.) meals are protein rich and inexpensive, but the presence of the polyphenolic dialdehyde, gossypol, is responsible of many toxic effects in animals including fishes. recently an effect on the transepithelial ion transport in rat colon has been demonstrated. in this study we investigated the effect of gossypol on the transepithelial electrical parameters of the isolated intestine of seawater adapted eel, anguilla anguilla, by employing a ussing chamber technique. we show ...200818606239
differential gene expression analysis in european eels (anguilla anguilla, l. 1758) naturally infected by macroparasites.we analyzed the relationships between the macroparasite community of the european eel and the expression of genes involved in the host physiology during its continental life. the genes studied are implicated in (1) host response to environmental stress, i.e., heat shock protein 70 (hsp70) and metallothionein (mt); (2) osmoregulation, i.e., beta thyroid hormone receptor (betathr) and na+/k+atpase; and (3) silvering, i.e., betathr, freshwater rod opsin (fwo), and deep-sea rod opsin (dso). all were ...200818605780
how cadmium could compromise the completion of the european eel's reproductive migration.the european eel (anguilla anguilla l.) is severely threatened with extinction. surprisingly, even though their unusual life cycle makes them particularly vulnerable to pollution, the possible contribution of contamination remains especially poorly known. here we have investigated the possible effect of cadmium (cd), a widespread nonessential metal, on eel reproductive capacities. both control and cd precontaminated female silver eels were experimentally matured and forced to swim in metal-free ...200818605594
cost of transport and optimal swimming speed in farmed and wild european silver eels (anguilla anguilla).a swimming speed of 0.4 meters per second (m s(-1)) is the minimal speed for european female silver eels to reach the spawning sites in the sargasso sea in time. as silver eels cease feeding when they start their oceanic migration, the cost of transport (cot) should be minimised and the swimming speed optimised to attain the highest energetic efficiency. in this study, we have investigated the optimal swimming speed (u(opt)) of silver eels since u(opt) may be higher than the minimal swimming spe ...200818599333
description of a new echinorhynchid species (acanthocephala) from the european eel, anguilla anguilla, in germany, with a key species of acanthocephalus in europe.acanthocephalus rhinensis n. sp. is described from the european eel. anguilla anguilla (linnaeus, 1758), collected in the rhine river near the city of karlsruhe, germany. it is the sixth species of acanthocephalus koelreuther, 1771 described from european fish. four other species are known from amphibians. the new species is distinguished from the other 5 species infecting fish by having a 1.2-mm-long proboscis armed with 15-21 rows of 13-16 hooks each, lemnisci about as long as receptacle, oblo ...200818576852
biased sex ratio in the european eel (anguilla anguilla) swim-bladder parasite anguillicola crassus, experimentally induced by 11-ketotestosterone.parasites are intimately connected to the host in which they live, and some may be affected by the polluted environment of their host. the present study describes the effect of a steroid hormone (11-ketotestosterone) on the sex ratio of the invasive hematophagous nematode anguillicola crassus kuwahara, niimi & itagaki, 1974, when experimentally injected to european eels, anguilla anguilla. our results showed that this steroid induced a significant male-biased ratio in the nematode a. crassus inf ...200818576815
biomonitoring of polybrominated diphenyl ether (pbde) pollution: a field study.polybrominated diphenyl ethers (pbdes) and cytochrome p450 enzyme activities were investigated in european eels (anguilla anguilla) collected from seven sites in a coastal lagoon in the north-western mediterranean sea, orbetello lagoon (italy). twelve pbde congeners were measured in muscle and two cyp1a enzyme activities, 7-ethoxyresorufin-o-deethylase (erod) and benzo(a)pyrene monooxygenase (bp(a)pmo), were investigated in liver microsomal fraction in order to obtain insights into the health of ...200818485829
characteristics of the peripheral blood of the anguillicola crassus (kuwahara, niimi et tagaki, 1974) and trypanosoma granulosum laveran et mesnil, 1902 infested eel (anguilla anguilla l.) ascending the river rega (north-western poland).the study was aimed at characterising the peripheral blood of eel (anguilla anguilla l.) ascending the river rega and infested with anguillicola crassus (kuwahara, niimi et itagaki, 1974) and trypanosoma granulosum laveran et mesnil, 1902. material and methods: haematological assays were performed on 58 eel individuals caught in august and september 2001 and in july 2002. the nematode anguillicola crassus was identified in the swim bladder lumen of 44 eel individuals. the mean infestation intens ...200718441878
interactions of 2,4,6-trinitrotoluene (tnt) with xenobiotic biotransformation system in european eel anguilla anguilla (linnaeus, 1758).the aim of the present study was to investigate the interaction of 2,4,6-trinitrotoluene (tnt) with liver biotransformation enzymes in european eel anguilla anguilla (linnaeus, 1758). eels were exposed to 0.5, 1 and 2.5mg/l nominal concentrations of tnt for 6 and 24h. modulation of cyp1a1, udpgt and gst genes was investigated by real-time pcr. total cyp450 content, nadph cytochrome c reductase activity, cyp1a and cyp2b-like activities, such as erod, mrod and brod, as well as gst and udpgt activi ...200818407354
brominated flame retardants and polychlorinated biphenyls in fish from the river scheldt, belgium.levels of polybrominated diphenyl ethers (pbdes), hexabromocyclododecanes (hbcds), and polychlorinated biphenyls (pcbs) were measured in several fish species originating from the river scheldt (belgium). five sampling locations were chosen in a highly industrialized area along the river, while two ponds in the vicinity of the river served as reference sites. the present study is a follow-up of a survey performed in 2000 which reported extremely high levels of pbdes and hbcds in eel (anguilla ang ...200818400299
effects of 2,4,6-trinitrotoluene (tnt) on phase i and phase ii biotransformation enzymes in european eel anguilla anguilla (linnaeus, 1758).the aim of this study was to investigate effects of the explosive 2,4,6-trinitrotoluene (tnt) on liver drug metabolizing genes and enzymes in the european eel anguilla anguilla as a model fish species. eels were exposed in vivo for 6h and 24h to 0.5, 1 and 2.5mg/l nominal concentrations of tnt. expression of cyp1a, glutathione-s-transferase (pi-class; gst) and uridine-diphosphate glucuronosyltransferase (1-family) (udpgt) genes was investigated by rt-pcr, and 7-ethoxy- and 7-methoxyresorufin-o-d ...200818378298
pressure and temperature interactions on aerobic metabolism in migrating silver eels: results in vitro.the european eel (anguilla anguilla) migrates (6000 km) from european coast towards the supposed spawning area: the sargasso sea. this intensive and sustained swimming activity is performed without feeding and by using essentially red muscle i.e. aerobic metabolism. temperature and hydrostatic pressure vary during migration and have known effects on energy metabolism, mainly on mitochondrial functioning. we raise the question about the existence of a pressure-temperature combination that optimiz ...200818351124
migratory response of european eel (anguilla anguilla) phagocytes to the eel swimbladder nematode anguillicola crassus.the migratory response of peripheral blood granulocytes and monocytes from the european eel anguilla anguilla (l.) to infective larvae of the swimbladder nematode anguillicola crassus kuwahara, niimi and hagaki, 1974 was examined by means of light microscopical histology and with an in vitro assay using a modified boyden chamber. histological examination of experimentally infected eels revealed that, already 8 days postinfection, an infiltration of inflammatory cells around l3 of a. crassus in t ...200818311570
is cl- protection against silver toxicity due to chemical speciation?in freshwater teleosts, the primary mechanism of acute silver toxicity is inhibition of na(+)/k(+) atpase and carbonic anhydrase at the gill, leading to net na(+) and cl(-) loss due to the continued diffusion of these ions into the hypoosmotic external environment. external cl(-) has been shown to protect rainbow trout (oncorhychus mykiss) against silver toxicity presumably by complexation to form agcl. however, cl(-) does not appear to greatly influence silver toxicity to at least two other spe ...200818304659
modulatory role of copper on β-naphthoflavone-induced dna damage in european eel (anguilla anguilla l.).gill and kidney dna integrity (alkaline unwinding assay) was assessed in anguilla anguilla exposed for 24-h to copper (cu: 1 or 2.5 μm), with or without 24-h pre-exposure to a polycyclic aromatic hydrocarbon (pah)-like compound--β-naphthoflavone (bnf: 2.7 μm). gill showed dna integrity loss in all the exposure conditions, reflecting a dual mode of bnf-cu interaction depending on the metal concentration. thus, antagonistic or additive effects were observed for bnf+cu 1 μm or bnf+cu 2.5 μm, respec ...200818304634
a field survey of metal binding to metallothionein and other cytosolic ligands in liver of eels using an on-line isotope dilution method in combination with size exclusion (se) high pressure liquid chromatography (hplc) coupled to inductively coupled plasma time-of-flight mass spectrometry (icp-tofms).the effect of metal exposure on the accumulation and cytosolic speciation of metals in livers of wild populations of european eel with special emphasis on metallothioneins (mt) was studied. four sampling sites in flanders showing different degrees of heavy metal contamination were selected for this purpose. an on-line isotope dilution method in combination with size exclusion (se) high pressure liquid chromatography (hplc) coupled to inductively coupled plasma time-of-flight mass spectrometry (i ...200818302969
vaccination of eels (anguilla japonica and anguilla anguilla) against anguillicola crassus with irradiated l3.the original host of the swimbladder nematode anguillicola crassus, the japanese eel (anguilla japonica) and the recently colonized european eel (anguilla anguilla) were immunized with 40 irradiated (500 gy) 3rd-stage larvae (l3) of this parasite and challenged with an infection of 40 normal l3. the immunization induced a significant reduction of the number of adult worms developing from the challenge infection in a. japonica, but not in a. anguilla. the induced resistance (calculated using the ...200818302804
first observations of histopathological effects of 2,4,6-trinitrotoluene (tnt) in gills of european eel anguilla anguilla (linnaeus, 1758): histopathological effects of 2,4,6-trinitrotoluene in gills of european eel.the aim of the present study was to investigate the effects on gill morphology of the explosive 2,4,6-trinitrotoluene (tnt) in a model fish, the european eel, anguilla anguilla (linnaeus, 1758) to assess potential detrimental effects in marine fish due to its presence in dumping areas. juvenile specimens of a. anguilla were exposed in vivo for 6 and 24 h to 0.5, 1 and 2.5 mg/l nominal concentrations of tnt using dimethyl sulfoxide (0.1 per thousand) as solvent carrier. histological analysis of g ...200818297406
offshore windmills and the effects of electromagnetic fields on fish.with the large scale developments of offshore windpower the number of underwater electric cables is increasing with various technologies applied. a wind farm is associated with different types of cables used for intraturbine, array-to-transformer, and transformer-to-shore transmissions. as the electric currents in submarine cables induce electromagnetic fields there is a concern of how they may influence fishes. studies have shown that there are fish species that are magneto-sensitive using geom ...200718240676
an investigation into the prevalence of renibacterium salmoninarum in farmed rainbow trout, oncorhynchus mykiss (walbaum), and wild fish populations in selected river catchments in england and wales between 1998 and 2000.a cross-sectional survey of renibacterium salmoninarum infection in farmed rainbow trout (rbt) and wild fish populations was carried out in 10 farms and six river catchments, respectively, in england and wales. the majority of the wild fish were sampled in 1998 and the farmed fish in 2000. grayling, thymallus thymallus, and brown trout, salmo trutta, were the main wild species sampled. two fish, one grayling and one salmon, salmo salar, were r. salmoninarum culture-positive, compared with 40 con ...200818234016
cadmium uptake by the european eel: trophic transfer in field and experimental investigations.due to its status of threatened species and being heavily contaminated by metals, the european eel (anguilla anguilla) was selected to investigate cadmium contamination levels of fish settled along a historically cadmium-contaminated hydrosystem, the garonne-gironde continuum (france), according to its various location sites and fish length. results have shown an important site effect on cadmium concentrations in liver but not in gills, highlighting the possible predominance of the trophic expos ...200818207239
effects of ph, sodium bicarbonate, cryoprotectants and foetal bovine serum on the cryopreservation of european eel sperm.the main objective of the present study was to evaluate the influence of ph and bicarbonate concentration in the activation or inhibition of european eel (anguilla anguilla) spermatozoa and to evaluate the effect of different cryoprotectants: dimethyl sulphoxide (dmso), acetamide, ethylene glycol, propanol, glycerol and methanol (meoh). the effect of these factors was evaluated comparing the percentage of motile cells, the percentage of alive cells (by hoechst staining) and the spermatozoa morph ...200818199265
european eel (anguilla anguilla l.) metallothionein, endocrine, metabolic and genotoxic responses to copper exposure.this study investigated anguilla anguilla (european eel) physiological and genotoxic responses to copper (cu) and their relation with metallothionein (mt) protection. eels were exposed during 7 days to cu 0.2 micromol/l. mt induction was assessed in gill and liver, revealing significant response only in liver. endocrine responses displayed a plasma free triiodothyronine (t3) and cortisol significant decrease, though the thyroid-stimulating hormone (tsh) and free thyroxine (t4) concentrations wer ...200818192012
pathogenesis of herpesvirus anguillae (hva) in juvenile european eel anguilla anguilla after infection by bath immersion.a clinical infection in post-larval (glass) european eels anguilla anguilla was successfully induced after artificial bath immersion with herpesvirus anguillae (hva), isolated from diseased european eel. hva caused a clinical infection after 7 d post-inoculation (pi); virus was detected by polymerase chain reaction (pcr) from day 1 pi; virus isolation was positive from day 7 pi, and hva antigen was detected by immunohistochemistry in gills and stomach from day 4 pi. tissue changes were found by ...200718159668
attempts to induce experimentally maturation of the gonads of the european eel, anguilla anguilla l. 194918133484
perfluoroalkyl compounds in danish wastewater treatment plants and aquatic environments.this study reports the results of a screening survey of perfluoroalkyl compounds (pfcs) in the danish environment. the study included point sources (municipal and industrial wastewater treatment plants and landfill sites) and the marine and freshwater environments. effluent and influent water and sewage sludge were analysed for point sources. sediment, blue mussels (mytilus edulis) and liver from plaice (pleuronectes platessa), flounder (platichthys flesus) and eel (anguilla anguilla) were analy ...200818029290
relationships between locomotor behavior, morphometric characters and thyroid hormone levels give evidence of stage-dependent mechanisms in european eel upstream migration.in order to decipher movements during freshwater eel colonization, we experimentally characterized individual locomotor behavior of two eel life history stages: elvers and yellow eels. a ramp located at the flume tank upstream side required a specific locomotor behavior to be ascended. placing individually tagged eels in the middle of the tank three times successively tested behavioral consistency. eels climbing the ramp on each trial were classified as "upstream climbers" whereas eels settling ...200817950736
differential expression of absorptive cation-chloride-cotransporters in the intestinal and renal tissues of the european eel (anguilla anguilla).duplicate pairs of isoforms of each of the nkcc2 and the ncc absorptive cation-chloride-cotransporters have been isolated from the european eel. as with mammalian nkcc2, nkcc2alpha isoform mrna expression was restricted to renal tissues, whereas nkcc2beta isoform expression was present in intestine and urinary bladder. similar to mammalian ncc, nccalpha mrna expression was also found in the kidney, whereas, expression of nccbeta mrna was found at low levels in a number of tissues but particularl ...200817950642
bioaccumulation of polychlorinated biphenyls in the eel (anguilla anguilla) at the camargue nature reserve - france.fish consumption is a potential source of human exposure to pollutants. here, we study residue levels of pcbs in the eel, anguilla anguilla, from the nature camargue reserve in southern france. chromatographic analysis (gc-ecd) found seventy identifiable congeners, among which, 10 are considered as dioxin-like pcbs, such as the non-ortho pcb 81 and the mono-ortho chlorobiphenyls pcb105, 114, 118, 123, 156, 157, 167, 170, 180. toxic equivalents (teq, who 2005 tef-toxic equivalent factors) varied ...200817900770
parasite communities of eels anguilla anguilla in freshwater and marine habitats in iceland in comparison with other parasite communities of eels in europe.ninety-five eels from one marine and three freshwater localities in iceland were examined for parasites. twenty species were found, 12 from marine habitat, 12 from freshwater and 4 species were found in both habitats. these are: eimeria anguillae, chilodonella hexasticha, trichodina fultoni, t. jadranica, myxidium giardi, myxobolus kotlani, two zschokkella spp., derogenes varicus, deropristis inflata, diplostonmum sp., plagioporus angulatus, podocotyle atomon, anisakis simplex (larva), eustrongy ...200717886743
probabilistic approach to polychlorinated biphenyl (pcb) exposure through eel consumption in recreational fishermen vs. the general population.concentrations of the sum of the seven indicator pcbs (sigma7 ipcbs) measured in non-commercial european eel (anguilla anguilla l.) in flanders are high: in 80% of all sampled localities, the belgian pcb standard for fish was exceeded. the objective of this study was to assess the intake of the sigma7 ipcbs through consumption of eel by recreational fishermen and to compare it to the intake of a background population. the median estimated intake for recreational fishermen varied between 18.4 and ...200717852389
spatial variations and temporal trends between 1994 and 2005 in polychlorinated biphenyls, organochlorine pesticides and heavy metals in european eel (anguilla anguilla l.) in flanders, belgium.in flanders, the northern region of belgium, european yellow eel muscle tissue was used as an indicator of environmental and potential human dietary exposure by hazardous chemicals of surface waters and sediments. between 1994 and 2005, over 2800 eel captured at 365 stations were analysed for pcbs, pesticides and heavy metals. contamination of eel in flanders fell within the range of reported concentrations in other watersheds of western europe. a spatial analysis of the data demonstrated that t ...200817825968
heavy metal distribution in tissues of six fish species included in human diet, inhabiting freshwaters of the nature park "hutovo blato" (bosnia and herzegovina).the aim of the study was to quantify heavy metal (mercury, lead, cadmium, and arsenic) concentration in tissues (muscles, liver, kidney, gills, and gonads) of six fish species (carp: cyprinus carpio, tench: tinca tinca, pumpkinseed: lepomis gibosus, prussian carp: carassius auratus gibelio, hasselquist: salmo dentex, eel: anguilla anguilla) from the freshwaters of the nature park hutovo blato, bosnia and herzegovina, and determine whether they are potentially harmful for human health if included ...200817687582
transcriptomic approach to the study of osmoregulation in the european eel anguilla anguilla.in euryhaline teleosts, osmoregulation is a fundamental and dynamic process that is essential for the maintenance of ion and water balance, especially when fish migrate between fresh water (fw) and sea water (sw) environments. the european eel has proved to be an excellent model species to study the molecular and physiological adaptations associated with this osmoregulatory plasticity. the life cycle of the european eel includes two migratory periods, the second being the migration of fw eels ba ...200717666525
element concentrations in the swimbladder parasite anguillicola crassus (nematoda) and its host the european eel, anguilla anguilla from asi river (hatay-turkey).the european eel's swimbladder nematode, anguillicola crassus, sampled from the asi river (orontes river) in antakya (hatay, turkey) in may 2006 were analysed by inductively coupled plasma-atomic emission spectrometry (icp-aes) for their some heavy metal (cd, cr, cu, fe, hg, mn, pb and zn) levels. the metal concentrations of the parasites were compared to different organs (swimbladder, liver, muscle and skin) of the fish hosts. the parasite contained statistically highly significantly amounts of ...200817661155
quality attributes of farmed eel (anguilla anguilla) stored under air, vacuum and modified atmosphere packaging at 0 degrees c.the shelf life of fresh eel in various packaging conditions of atmospheric air, vacuum and modified atmosphere packaging (map) (40% co(2), 30% n(2) and 30% o(2)) at 0 degrees c was investigated. all raw eel samples received acceptable sensory scores during the first 11+/-1 days of storage in atmospheric air, 11+/-1 days of storage in vacuum and finally 18+/-1 days of storage in map conditions. using the microbial quality indicators the shelf life of eel packed in air, vacuum and map was estimate ...200717613370
nitric oxide modulates ionic transport in the isolated intestine of the eel, anguilla anguilla.we investigated the role of no (nitric oxide) in the isolated intestine of the sea water adapted eel, by testing the effect of various donors on i(sc) (short-circuit current), due to net cl(-) absorption in the control conditions. we found that the endogenous no-synthase substrate l-arginine as well as two different no donors, snp (sodium nitroprusside) and sin-1 (3-morpholinosydnonimine), produced a slow and gradual decrease of i(sc). the effect of snp was reduced by the pretreatment with odq ( ...200717604669
do eel parasites reflect the local crustacean community? a case study from the rhine river system.in 2003, the parasite fauna of 197 european eels anguilla anguilla, captured at three different locations (laufenburg, karlsruhe and beneeden leeuwen) in the river rhine, was analysed. the eels harboured a total of 18 species, among them the protozoa (myxidium giardi, myxobolus kotlani and trypanosoma granulosum), acanthocephalans (acanthocephalus anguillae, acanthocephalus lucii, echinorhynchus truttae, pomphorhynchus laevis), nematodes (paraquimperia tenerrima, pseudocapillaria tomentosa, cama ...200717578598
energy and migratory behavior in glass eels (anguilla anguilla).the influence of energy content of glass eels (anguilla anguilla) on their migratory behavior was investigated in november 2004 and february 2005 under laboratory conditions. fish were sorted according to their response to a decrease in light intensity: glass eels becoming active at dusk and using water flow to move with the current were considered as "active", whereas those remaining in the substratum throughout the catching period (24 h) were categorized as "inactive". "active" glass eels exhi ...200717561177
to what extent are hepatic concentrations of heavy metals in anguilla anguilla at a site in a contaminated estuary related to body size and age and reflected in the metallothionein concentrations?we explored how hepatic [metal]s in anguilla anguilla at a contaminated estuarine site are influenced by body size, age and season, and the extent that [cu], [cd] and [zn]s are reflected in [metallothionein (mt)]s. although each [metal] and [mt] increased significantly with length, weight and age, those biotic variables explained <10% of the variation in each [metal] and only 11-16% for [mt]. seasonal changes in [cu] and [cd] were paralleled by [mt]. the variation in [mt] explained by cu (42%) w ...200817548139
methylmercury bioconcentration in muscle tissue of the european eel (anguilla anguilla) from the adour estuary (bay of biscay, france). 200717543349
common pattern of gene expression in response to hypoxia or cadmium in the gills of the european glass eel (anguilla anguilla).european eel (anguilla anguilla) populations are in decline. glass eel recruitment has fallen 10-fold since the early 1980s. estuaries play a fundamental role in the life history of eels because glass eels must pass through them to reach freshwater ecosystems. unfortunately, because of their geographical position at the upstream basin slopes, estuaries accumulate metals like cadmium and are important sites of hypoxia events. in this context, we studied the effect of the oxygen level on the venti ...200717533871
biochemical characterization of a s-glutathionylated carbonic anhydrase isolated from gills of the antarctic icefish chionodraco hamatus.gill cytoplasmic carbonic anhydrase of the haemoglobinless antarctic icefish chionodraco hamatus (ice-ca) was directly sequenced and consists in 259 residues with an acetylated n-terminus. the molecular mass, deduced from the sequence, was 28.45 kda, while mass spectrometry analysis of the native protein gave higher values. treatment with dithiothreitol abolished this difference, indicating possible post-translational modifications. isoelectric focusing analysis of ice-ca suggested s-thiolation, ...200717510781
the pathogenic helminth parasites of eels.although 63 and 55 species of helminths have been reported from each species of atlantic eel and from 29 to 19 for each species of pacific eel only the monogeneans pseudodactylogyrus bini and p. anguillae and the nematode anguillicola crassus, originally specific to species of pacific eels, can be considered serious pathogens. none of the three are normally pathogenic to their preferred natural eel host species in the wild. pseudodactylogyrus spp. only cause serious local gill damage when presen ...200717498176
spermiogenesis and sperm ultrastructure of deropristis inflata molin, 1859 (digenea, deropristidae), a parasite of anguilla anguilla.to our knowledge, this paper describes the first ultrastructural results on spermiogenesis and the spermatozoon of a digenea belonging to the family deropristidae, deropristis inflata. spermiogenesis follows the usual pattern found in the digeneans, but a single noteworthy characteristic concerns the centriole, which presents a "prominent pear-shaped electron-dense region." spermiogenesis in d. inflata begins with the formation of a differentiation zone. the two centrioles give rise to flagella. ...200717484073
does a 5500-km swim trial stimulate early sexual maturation in the european eel (anguilla anguilla l.)?the catadromous european eel (anguilla anguilla l.) undertakes a 6000-km spawning migration from its freshwater habitats to the sargasso sea. in large blazka swim tunnels of 127 l, the physiological effect of such a prolonged swimming performance on sexual maturation in adult female eels was investigated. two groups of eels were placed in swim tunnels for 173 days, one group was able to swim at 0.5 body lengths/second (swim group) covering a distance of c. 5500-km over the experimental period, a ...200717451986
environmental stress and life-stage dependence on the detection of heterozygosity-fitness correlations in the european eel, anguilla anguilla.heterozygosity-fitness correlations (hfcs) have been reported in populations of many species, although hfcs can clearly vary across species, conspecific populations, temporal samples, and sexes. we studied (i) the temporal stability of the association between genetic variation and growth rate (length and mass increase) and (ii) the influence of genetic variability on survival in the european eel (anguilla anguilla l). hfcs were assessed using genotypes from 10 allozyme and 6 microsatellite marke ...200617426758
detection of hexabromocyclododecane and its metabolite pentabromocyclododecene in chicken egg and fish from the official food control.during routine gas chromatography with electron capture detection (gc/ecd) analysis of chicken eggs, we observed that the most prominent peak in some samples did not match the retention time of any of the food contaminants screened. subsequent gc coupled with mass spectrometry (gc/ms) studies clarified that the mass spectrum of the peak was very similar to hexabromocyclododecane (hbcd), which was also identified by gc/ms in the egg. the unknown compound was positively identified as pentabromocyc ...200717419640
development of real-time rt-pcr assays for eel gonadotropins and their application to the comparison of in vivo and in vitro effects of sex steroids.gonadotropins, luteinizing hormone (lh) and follicle-stimulating hormone (fsh), are key factors in the brain-pituitary-gonad axis and understanding their regulation remains essential for future management of eel reproduction. in this regard, we developed quantitative real-time rt-pcr (qrtrt-pcr) assays for the expression of european eel lhbeta, fshbeta and gpalpha subunits, using the light cycler system. the qrtrt-pcr was adapted to permit detection of the three gonadotropin subunit mrnas in ind ...200917418843
dioxins and dioxin-like pcbs in different fish from the river elbe and its tributaries, germany.in a long-term program polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (pcdd/fs) as well as dioxin-like polychlorinated biphenyls (dl-pcbs) were analyzed in the muscle tissue of eels (anguilla anguilla), bream (abramis brama), european chub (leuciscus cephalus) and ide (leuciscus idus) from the river elbe and its tributaries mulde and saale. the variation of the pcdd/f and dl-pcb concentrations in all fish samples is very large, whereby the dl-pcbs predominate in comparison t ...200717382467
cortisol regulates eel (anguilla anguilla) aquaporin 3 (aqp3) mrna expression levels in gill.previous studies in eel (anguilla anguilla) gill have shown that the expression of the aquaporin 3 (aqp3) water and small solute channel is dramatically decreased (mrna abundance decreased by up to 97%) when these euryhaline fish are acclimated from freshwater (fw) to seawater (sw). however, aqp3 mrna expression levels in the intestine following sw-acclimation do not change. the sw-acclimating corticosteroid hormone, cortisol has previously been shown to regulate the expression of aquaporins (pa ...201317353012
effect of sperm cryopreservation on the european eel sperm viability and spermatozoa morphology.the main objective of the present work was to study the effect of cryopreservation of european eel sperm both on the sperm viability and the spermatozoa head morphology. spermatozoa morphology was evaluated with computer-assisted morphology analysis after collection in fresh samples, after adding the freezing medium containing dimethyl sulfoxide as cryoprotectant and, finally, after the cryopreservation process and thawing. cell viability was assessed, in both fresh and thawed samples, by hoechs ...200717348973
effects of high hydrostatic pressure on the pituitary-gonad axis in the european eel, anguilla anguilla (l.).european silver eels are thought to undergo sexual maturation during their oceanic reproductive migration from the european continent to their spawning area in the sargasso sea. tracking data and various anatomical and physiological features suggest that silver eels migrate in deep sea, leading us to hypothesise that high hydrostatic pressure (hp) influences the induction of eel reproduction. we subjected female and male silver eels to 101ata for 3 and 7 weeks, respectively, in a hyperbaric cham ...201517324430
salinity adaptation and gene profiling analysis in the european eel (anguilla anguilla) using microarray technology.the life cycle of the european eel (anguilla anguilla) includes two long migratory periods, when the newly hatched leptocephali larvae drift on ocean currents from the sargasso sea to the shores of western europe and then again up to 30 years later when adult eels swim back to their place of birth for reproductive purposes. prior to the migration from fresh water (fw) to sea water (sw) adult yellow eels undergo various anatomical and physiological adaptations (silvering) which promote sexual dev ...200717324422
the role of volume-sensitive ion transport systems in regulation of epithelial transport.this review focuses on using the knowledge on volume-sensitive transport systems in ehrlich ascites tumour cells and nih-3t3 cells to elucidate osmotic regulation of salt transport in epithelia. using the intestine of the european eel (anguilla anguilla) (an absorptive epithelium of the type described in the renal cortex thick ascending limb (ctal)) we have focused on the role of swelling-activated k+- and anion-conductive pathways in response to hypotonicity, and on the role of the apical (lumi ...200717289411
thyroid hormone-induced demineralisation of the vertebral skeleton of the eel, anguilla anguilla.the role of thyroid hormones (th) in bone remodelling is controversial. indeed, in humans, while they are necessary for normal growth and development, their overproduction can induce important mineral bone loss and osteoporosis. intense bone resorption is a natural phenomenon also observed in some teleosts, during reproductive migration and fasting. our work aimed at investigating the effects of chronic treatments with th (thyroxin, t4 or triiodothyronine, t3) on bone resorption in a migratory f ...200717280664
impairment of lipid storage by cadmium in the european eel (anguilla anguilla).because european silver eels (anguilla anguilla) fast during their reproductive migration to the sargasso sea, the successful completion of their unusual life cycle depends on quantity of lipids stored beforehand. these lipids are mainly accumulated during the growth phase stage of the animals, called yellow eel, as triglycerides in muscle. they are then catabolized to provide sufficient energy to enable migration, gonad maturation and spawning. in the laboratory, we investigated the possible im ...200717276523
occurrence of edwardsiella tarda in wild european eels anguilla anguilla from mediterranean spain.pure cultures of edwarsiella tarda were isolated from body ulcers and internal organs of wild european eels caught in a mediterranean freshwater coastal lagoon (albufera lake, valencia, spain) over a 1 yr period. overall, the e. tarda isolation rate from wild eels was 9%, but this increased to 22.8% in diseased individuals. all 22 e. tarda isolates belonged to the 'wild-type' biogroup of the species and were virulent for eels (lethal dose that kills 50% of exposed individuals [ld50 dose]: 10(4.8 ...200617240756
genotyping of marine viral haemorrhagic septicaemia virus isolated from the flemish cap by nucleotide sequence analysis and restriction fragment length polymorphism patterns.a total of 14 viral haemorrhagic septicaemia virus (vhsv) isolates obtained from greenland halibut reinhardtius hippoglossoides caught at the flemish cap, a fishing ground in the north atlantic ocean near newfoundland, were characterised using restriction fragment length polymorphism (rflp) and nucleotide sequence analysis. rflp analysis was performed on a 1259 bp fragment of the glycoprotein (g) gene, and a 305 nucleotide region within the nucleoprotein (n) gene was used for sequence analysis. ...200617240749
ultrastructure of the spermatozoon of bothriocephalus claviceps (cestoda, pseudophyllidea): a parasite of anguilla anguilla (fish, teleostei).the mature bothriocephalus claviceps spermatozoon is filiform and tapered at both extremities. it contains two axonemes of unequal lengths of the 9+"1" pattern of the trepaxonemata. the anterior extremity exhibits an apical cone of an electron-dense material about 6.6 microm long by 0.2 microm wide at its base and a crest-like body making an angle of about 40 degrees with the spermatozoon axis. it is spiralized around the whole extremity of the spermatozoon. the nucleus is a cord of electron-den ...200717235546
stocking density at early developmental stages affects growth and sex ratio in the european eel (anguilla anguilla).to investigate the effect of stocking density on growth and sex ratio in european eel, four constant density conditions were tested during the transition from the glass to the elver stage for 90 days (period 1). the test conditions combined the weight of fish per unit surface or volume (surface density or volume density) resulting in four experimental groups: low surface density (0.5 kg/m(2)) and low volume density (5 kg/m(3)) (group s(0.5)v(5)); low surface density (0.5 kg/m(2)) and high volume ...200617179387
metal and metallothionein content in tissues from wild and farmed anguilla anguilla at commercial size.metallothionein and metal content (cd, zn, hg, cu, fe, pb and mn) were determined in various organs of commercially available eel (anguilla anguilla) of similar size obtained from a local farm and from the albufera lake in valencia (spain). farmed fish showed statistically significant higher cd concentrations in liver and kidney whereas wild individuals had higher levels of pb in blood and zn in kidney. significant positive correlations were found between metallothionein and cd in kidney of farm ...200717157913
endocrine and metabolic responses of anguilla anguilla l. caged in a freshwater-wetland (pateira de fermentelos--portugal).the present short-term in situ study was carried out in a freshwater-wetland--pateira de fermentelos--considered an enlargement of cértima river, in the centre of portugal. this ecosystem is an important fishing and recreation place, receiving effluents from different origins namely, electroplating industrial effluents containing heavy metals, domestic wastes, as well as pesticides and fertilizers resulting from agriculture activities. the aim of the present research work was to monitor the effe ...200717150242
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