Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
severe fever with thrombocytopenia syndrome in japan and public health communication.a fatal case of severe fever with thrombocytopenia syndrome was reported in japan in 2013. the ensuing process of public communication offers lessons on how to balance public health needs with patient privacy and highlights the importance of multilateral collaborations between scientific and political communities.025695132
transcriptomic responses to emamectin benzoate in pacific and atlantic canada salmon lice lepeophtheirus salmonis with differing levels of drug resistance.salmon lice lepeophtheirus salmonis are an ecologically and economically important parasite of wild and farmed salmon. in scotland, norway, and eastern canada, l. salmonis have developed resistance to emamectin benzoate (emb), one of the few parasiticides available for salmon lice. drug resistance mechanisms can be complex, potentially differing among populations and involving multiple genes with additive effects (i.e., polygenic resistance). indicators of resistance development may enable early ...201425685190
antiviral responses of arthropod vectors: an update on recent advances.arthropod vectors, such as mosquitoes, ticks, biting midges and sand flies, transmit many viruses that can cause outbreaks of disease in humans and animals around the world. arthropod vector species are invading new areas due to globalisation and environmental changes, and contact between exotic animal species, humans and arthropod vectors is increasing, bringing with it the regular emergence of new arboviruses. for future strategies to control arbovirus transmission, it is important to improve ...201425674592
strategic applications of long-acting acaricides against rhipicephalus (boophilus) microplus in northwestern argentina, with an analysis of tick distribution among cattle.strategic applications of long-acting acaricides for the control of rhipicephalus (boophilus) microplus in northwestern argentina were evaluated for one year. in addition, tick distribution among cattle was analyzed to evaluate if partial selective treatment or culling the small proportion of most heavily infested animals were feasible options to control r. (b.) microplus. two different treatments schemes based on two applications of fluazuron and one application of 3.15% ivermectin were perform ...201525670006
histoarchitecture of the ovary of rhipicephalus (boophilus) annulatus during pre- and postengorgement period.the present communication describes the detailed day wise study of histological changes of the ovary of rhipicephalus (boophilus) annulatus in the postengorgement period together with the systematic classification of their oocytes. the ovary of r. (b.) annulatus is panoistic type with an asynchronous development of oocytes. all the stages (ii, iii, iv, and v) of oocytes except stage i were similar to r. (b.) microplus. the stage i oocytes showed basophilia, which was not reported earlier in othe ...201525664337
esterase and glutathione s-transferase levels associated with synthetic pyrethroid resistance in hyalomma anatolicum and rhipicephalus microplus ticks from punjab, india.larval packet test was used for assessment of resistance status against cypermethrin and deltamethrin in hyalomma anatolicum and rhipicephalus microplus from various districts of punjab (india). among the various field isolates of h. anatolicum susceptible status was recorded against cypermethrin in all isolates, whereas against deltamethrin resistance status (level i-iii) was recorded. in r. microplus lower resistance levels (i-ii) were recorded against cypermethrin in comparison to deltamethri ...201525652614
native strains of beauveria bassiana for the control of rhipicephalus sanguineus sensu lato.rhipicephalus sanguineus sensu lato ticks are widespread worldwide due to their adaptability to survive under different environmental conditions. they may act as vectors of a wide range of pathogens to humans and animals and their control is based on the use of chemical products on dogs and in the environment. alternative control strategies, such as the use of entomopathogenic fungi as bio-control agents have also been investigated. the ability of native strains of beauveria bassiana sensu lato ...201525651851
bovine genetic resistance effects on biological traits of rhipicephalus (boophilus) microplus.this study aimed to verify the influence of bovine genetic resistance on biological traits of the rhipicephalus (boophilus) microplus tick. genetic resistance or susceptibility was determined according to breeding values for tick counts, predicted using a dataset of 9007 hereford and braford (hereford×zebu) bovines naturally infested and raised under extensive production systems in southern brazil. from a total of 974 braford heifers born in 2008, 20 were classified as genetically tick-resistant ...201525648284
insecticidal and repellent activity of siparuna guianensis aubl. (negramina) against aedes aegypti and culex quinquefasciatus.this study investigated the toxic effects of essential oils isolated from siparuna guianensis against aedes aegypti, culex quinquefasciatus (eggs, larvae, pupae, and adult) and aedes albopictus (c6/36) cells. the oviposition-deterring activity, egg viability, and repellence activity in the presence of different essential oils concentrations were determined. the essential oils showed high toxicity to all developmental stages of a. aegypti and c. quinquefasciatus. furthermore, the oils also showed ...201525646797
rickettsial infection in animals, humans and ticks in paulicéia, brazil.a previous study in paulicéia municipality, south-eastern brazil, reported 9.7% of the amblyomma triste ticks to be infected by rickettsia parkeri, a bacterial pathogen that causes spotted fever in humans. these a. triste ticks were shown to be associated with marsh areas, where the marsh deer blastocerus dichotomus is a primary host for this tick species. during 2008-2009, blood serum samples were collected from 140 horses, 41 dogs, 5 opossums (didelphis albiventris) and 26 humans in farms from ...201525643912
an evolutionarily-unique heterodimeric voltage-gated cation channel found in aphids.we describe the identification in aphids of a unique heterodimeric voltage-gated sodium channel which has an atypical ion selectivity filter and, unusually for insect channels, is highly insensitive to tetrodotoxin. we demonstrate that this channel has most likely arisen by adaptation (gene fission or duplication) of an invertebrate ancestral mono(hetero)meric channel. this is the only identifiable voltage-gated sodium channel homologue in the aphid genome(s), and the channel's novel selectivity ...201525637326
unprecedented genomic diversity of rna viruses in arthropods reveals the ancestry of negative-sense rna viruses.although arthropods are important viral vectors, the biodiversity of arthropod viruses, as well as the role that arthropods have played in viral origins and evolution, is unclear. through rna sequencing of 70 arthropod species we discovered 112 novel viruses that appear to be ancestral to much of the documented genetic diversity of negative-sense rna viruses, a number of which are also present as endogenous genomic copies. with this greatly enriched diversity we revealed that arthropods contain ...201525633976
in vitro acaricidal activity of murraya koenigii (l.) spreng (rutaceae) extracts against synthetic pyrethroid-resistant rhipicephalus (boophilus) microplus.larval packet test was used for detection of resistance status against cypermethrin and deltamethrin, the most commonly used synthetic pyrethroids in rhipicephalus (boophilus) microplus collected from faridkot district, punjab (india). the slope of mortality, lethal concentration for 50 % (lc50) and resistance levels were determined from the regression graphs of probit mortality of ticks plotted against log values of increasing concentrations of cypermethrin and deltamethrin. results indicated p ...201525633856
detection of spotted fever group rickettsiae in ticks from zhejiang province, china.tick species distribution and prevalence of spotted fever group rickettsiae (sfgr) in ticks were investigated in zhejiang province, china in 2010 and 2011. pcr was used to detect sfgr and positive amplicons were sequenced, compared to published sequences and phylogenic analysis was performed using mega 4.0. a total of 292 adult ticks of ten species were captured and 7.5 % (22/292) of the ticks were pcr-positive for sfg rickettsia. the pcr-positive rates were 5.5 % (6/110) for haemaphysalis longi ...201525633265
natural babesia bovis infection in water buffaloes (bubalus bubalis) and crossbred cattle under field conditions in egypt: a preliminary study.there is a little or no data available on the natural babesia bovis (b. bovis) infection in water buffaloes (bubalus bubalis) comparing to the available one for cattle. this study was conducted to investigate the natural b. bovis infection in water buffaloes in comparison to crossbred cattle under field conditions in egypt.201325629060
potentially novel ehrlichia species in horses, nicaragua.ehrlichia sp. dna was amplified from 4 ehrlichia-seroreactive horses from mérida, nicaragua. sequencing of 16s rdna, sodb, and groel genes indicated that the bacterium is most likely a novel ehrlichia species. the tick vector and the potential for canine and human infection remain unknown.201525625228
role of rhipicephalus microplus cheliceral receptors in gustation and host differentiation.rhipicephalus microplus is considered the most economically important ectoparasite of cattle worldwide. it is known that zebuine breeds of cattle are less susceptible to tick infestation than taurine breeds. contact chemoreceptors in the cheliceral pit sensilla of ticks respond selectively to phagostimulant compounds, however their role in blood feeding relative to host susceptibility to infestation remains to be fully understood. we addressed this topic by conducting taste electrophysiology exp ...201525616714
investigation of tick-borne bacteria (rickettsia spp., anaplasma spp., ehrlichia spp. and borrelia spp.) in ticks collected from andean tapirs, cattle and vegetation from a protected area in ecuador.ixodid ticks play an important role in the transmission and ecology of infectious diseases. information about the circulation of tick-borne bacteria in ticks is lacking in ecuador. our aims were to investigate the tick species that parasitize andean tapirs and cattle, and those present in the vegetation from the buffer zone of the antisana ecological reserve and cayambe-coca national park (ecuador), and to investigate the presence of tick-borne bacteria.201525616567
complete genome sequence of ehrlichia mineirensis, a novel organism closely related to ehrlichia canis with a new host association.we report here the complete genome sequencing of ehrlichia mineirensis, an ehrlichia organism that was isolated from the hemolymph of rhipicephalus microplus-engorged females. e. mineirensis is the best characterized ehrlichia isolate from a novel cattle-related clade closely related to the monocytotropic pathogen e. canis.201525614563
theileria equi isolates vary in susceptibility to imidocarb dipropionate but demonstrate uniform in vitro susceptibility to a bumped kinase inhibitor.the apicomplexan hemoparasite theileria equi is a causative agent of equine piroplasmosis, eradicated from the united states in 1988. however, recent outbreaks have sparked renewed interest in treatment options for infected horses. imidocarb dipropionate is the current drug of choice, however variation in clinical response to therapy has been observed.201525600252
non-classical gluconeogenesis-dependent glucose metabolism in rhipicephalus microplus embryonic cell line bme26.in this work we evaluated several genes involved in gluconeogenesis, glycolysis and glycogen metabolism, the major pathways for carbohydrate catabolism and anabolism, in the bme26 rhipicephalus microplus embryonic cell line. genetic and catalytic control of the genes and enzymes associated with these pathways are modulated by alterations in energy resource availability (primarily glucose). bme26 cells in media were investigated using three different glucose concentrations, and changes in the tra ...201525594873
tick-borne haemoparasites in african buffalo (syncerus caffer) from two wildlife areas in northern botswana.the african buffalo (syncerus caffer) is a host for many pathogens known to cause economically important diseases and is often considered an important reservoir for livestock diseases. theileriosis, heartwater, babesiosis and anaplasmosis are considered the most important tick-borne diseases of livestock in sub-saharan africa, resulting in extensive economic losses to livestock farmers in endemic areas. information on the distribution of tick-borne diseases and ticks is scarce in northern botswa ...201525589064
molecular characterization of a defensin gene from a hard tick, dermacentor silvarum.ticks are distributed worldwide and considered as vectors of many human diseases. tick defensins, a family of antimicrobial peptides, form the first line of defense against pathogens.201525588982
tick-borne pathogens and the vector potential of ticks in china.ticks, as obligate blood-sucking ectoparasites, attack a broad range of vertebrates and transmit a great diversity of pathogenic microorganisms. they are considered second only to mosquitoes as vectors of human disease, and the most important vector of pathogens of domestic and wild animals. of the 117 described species in the chinese tick fauna, 60 are known to transmit one or more diseases: 36 species isolated within china and 24 species isolated outside china. moreover, 38 of these species ca ...201525586007
integrated control of an acaricide-resistant strain of the cattle tick rhipicephalus microplus by applying metarhizium anisopliae associated with cypermethrin and chlorpyriphos under field conditions.the efficacy of the fungus metarhizium anisopliae to control ticks has been shown in several in vitro experiments. however, few studies have been undertaken in field conditions in order to demonstrate the applicability of its use as a biological control of ticks and its combination with chemical acaricides. the aim of the present study was to evaluate the efficacy of m. anisopliae to control an acaricide-resistant strain of rhipicephalus microplus under laboratory and field conditions. first, th ...201525577676
susceptibility of rhipicephalus (boophilus) microplus to ivermectin (200, 500 and 630 μg/kg) in field studies in brazil.the present study aimed to determine the susceptibility of 17 rhipicephalus (boophilus) microplus populations, originating in the southeast and southern regions of brazil, to different ivermectin concentrations (200, 500 and 630 μg/kg), administered through subcutaneous or topical (pour-on) routes. r. (b.) microplus populations from the states of minas gerais (seven populations), são paulo (seven populations) and paraná (three populations) were chosen for the tests. the selected cattle were allo ...201525576441
cattle ticks in cameroon: is rhipicephalus (boophilus) microplus absent in cameroon and the central african region?in most parts of the world, ticks are rapidly developing resistance to commonly used acaricides thus rendering control difficult. this constraint is further compounded by the introduction of new species in areas where they did not exist before. such is the case with the introduction into and rapid spread of rhipicephalus (boophilus) microplus in some countries of west africa. with the looming threat of its further spread in the region, the objective of the present study was to update knowledge o ...201525575435
evolutionary process of bos taurus cattle in favourable versus unfavourable environments and its implications for genetic selection.the evolutionary processes that have enabled bos taurus cattle to establish around the globe are at the core to the future success of livestock production. our study focuses on the history of cattle domestication including the last 60 years of b. taurus breeding programmes in both favourable and unfavourable environments and its consequences on evolution and fitness of cattle. we discuss the emergence of 'production diseases' in temperate production systems and consider the evolutionary genetics ...025567936
rhipicephalus microplus serine protease inhibitor family: annotation, expression and functional characterisation assessment.rhipicephalus (boophilus) microplus evades the host's haemostatic system through a complex protein array secreted into tick saliva. serine protease inhibitors (serpins) conform an important component of saliva which are represented by a large protease inhibitor family in ixodidae. these secreted and non-secreted inhibitors modulate diverse and essential proteases involved in different physiological processes.201525564202
reverse genetics system for severe fever with thrombocytopenia syndrome virus.severe fever with thrombocytopenia syndrome virus (sftsv) is an emerging tick-borne pathogen that was first reported in china in 2009. phylogenetic analysis of the viral genome showed that sfts virus represents a new lineage within the phlebovirus genus, distinct from the existing sandfly fever and uukuniemi virus groups, in the family bunyaviridae. sfts disease is characterized by gastrointestinal symptoms, chills, joint pain, myalgia, thrombocytopenia, leukocytopenia, and some hemorrhagic mani ...201425552716
development of a multilocus sequence typing scheme for the study of anaplasma marginale population structure over space and time.bovine anaplasmosis caused by anaplasma marginale is a worldwide disease prevalent in tropical and subtropical regions where rhipicephalus microplus is considered the most significant biological vector. molecular markers previously applied for a. marginale typing are efficient for isolate discrimination but they are not a suitable tool for studying population structure and dynamics. here we report the development of an mlst scheme based on the study of seven genes: dnaa, ftsz, groel, lipa, reca, ...201525550150
prevalence of borrelia burgdorferi sensu lato in ticks from eastern china.to explore the tick distribution and prevalence of borrelia in zhejiang province, we performed a survey in nine sites. a total of 447 adult ticks of 11 species were captured and the dominant tick species were haemaphysalis longicornis and ixodes sinensis and the abundance of tick species in different areas varied significantly. overall, 4.70% of the ticks were polymerase chain reaction (pcr) positive for borrelia. the average pcr positive rates were 5.19% for h. longicornis, 3.45% for amblyomma ...201525548382
3β-o-tigloylmelianol from guarea kunthiana: a new potential agent to control rhipicephalus (boophilus) microplus, a cattle tick of veterinary significance.chemical investigation of guarea kunthiana fruits, guided by their effect on the reproductive cycle of engorged females of the cattle tick rhipicephalus (boophilus) microplus-a major economic problem to the livestock industry worldwide-led to isolation of 3β-o-tigloylmelianol, a new protolimonoid, from the bioactive hexane phase obtained by partitioning the crude ethanol extract. an adult immersion test was performed. the compound strongly inhibited egg-laying and hatchability (99.2% effectivene ...201425546624
co-transmission of the non-transmissible south african babesia bovis s24 vaccine strain during mixed infection with a field isolate.the south african babesia bovis live blood vaccine, originating from a field isolate attenuated by 23 serial syringe passages in splenectomized calves, has lost the ability to infect the natural vector rhipicephalus (boophilus) microplus. in this study, infection with mixed parasites from the vaccine strain and a field isolate, resulted in transmission of both genotype populations. comparing the field isolate and transmitted combination indicated no significant difference in their virulence, whi ...201525544307
anopheles gambiae salivary protein expression modulated by wild plasmodium falciparum infection: highlighting of new antigenic peptides as candidates of an. gambiae bites.malaria is the major parasitic disease worldwide caused by plasmodium infection. the objective of integrated malaria control programs is to decrease malaria transmission, which needs specific tools to be accurately assessed. in areas where the transmission is low or has been substantially reduced, new complementary tools have to be developed to improve surveillance. a recent approach, based on the human antibody response to anopheles salivary proteins, has been shown to be efficient in evaluatin ...201425526764
in vitro evaluation of ethanolic extracts of ageratum conyzoides and artemisia absinthium against cattle tick, rhipicephalus microplus.in vitro efficacy of ethanolic extracts obtained from the aerial parts of ageratum conyzoides and artemisia absinthium was assessed on rhipicephalus microplus using adult immersion test (ait). five concentrations of the extract (1.25%, 2.5%, 5%, 10%, and 20%) with three replications for each concentration were used in the bioassay. in ait, the maximum mortality was recorded as 40% and 66.7% at 20% concentration for a. conyzoides and a. absinthium, respectively. acaricidal activity was found to b ...201425525626
effect of a spray formulation on the reproductive parameters of a susceptible population of rhipicephalus (boophilus) microplus.the number of studies emphasizing the possible damage that acaricidal spray formulations can cause on engorged female ticks' reproductive parameters is small. the present study evaluated the deleterious effects of a spray formulation (dichlorvos 60% + chlorpyrifos 20%) on the reproductive parameters of a susceptible population of rhipicephalus (b.) microplus females, using the stall test. the ticks were allocated randomly to treatments according to the mean numbers of females detached from each ...201725517518
in vitro acaricidal activity of atropa belladonna and its components, scopolamine and atropine, against rhipicephalus (boophilus) microplus.in vitro efficacy of methanolic extract of atropa belladonna and its components scopolamine and atropine was assessed against rhipicephalus (boophilus) microplus. five concentrations of the extract (1.25%, 2.5%, 5%, 10%, and 20%) were used whereas scopolamine and atropine were each tested at 0.1%. in adult immersion test, the extract was lethal to ticks at 20% concentration. the lc50 and lc95 values were determined as 6.875% and 17.306%, respectively. the extract caused a significant reduction ( ...201425516877
transmission-blocking vaccines: focus on anti-vector vaccines against tick-borne diseases.tick-borne diseases are a potential threat that account for significant morbidity and mortality in human population worldwide. vaccines are not available to treat several of the tick-borne diseases. with the emergence and resurgence of several tick-borne diseases, emphasis on the development of transmission-blocking vaccines remains increasing. in this review, we provide a snap shot on some of the potential candidates for the development of anti-vector vaccines (a form of transmission-blocking v ...201425503555
the glycoprotein trp36 of ehrlichia sp. ufmg-ev and related cattle pathogen ehrlichia sp. ufmt-bv evolved from a highly variable clade of e. canis under adaptive diversifying selection.a new species of ehrlichia, phylogenetically distant from e. ruminantium, was found in 2010 infecting cattle in canada. in 2012 and 2013, we reported the in vitro propagation, molecular and ultrastructural characterization of ehrlichia sp. ufmg-ev (e. mineirensis), a new species of ehrlichia isolated from the haemolymph of brazilian rhipicephalus (boophilus) microplus ticks. a new organism, named ehrlichia sp. ufmt-bv, closely related to ehrlichia sp. ufmg-ev, was recently described in brazil an ...201425499826
inducible expression of mu-class glutathione s-transferases is associated with fenpropathrin resistance in tetranychus cinnabarinus.the carmine spider mite, tetranychus cinnabarinus (boisduval), is a serious pest on a variety of economically important crops widely distributed in china, and its resistance to acaricides has quickly developed. in this study, we fully sequenced 13 gst genes of t. cinnabarinus (tcgsts). the phylogenetic tree showed that five of them belonged to the delta class and the other eight belonged to the mu class. the alignment of gene sequences and comparison of gene expressions between a fenpropathrin-r ...201425493473
immunoprotective potential of a rhipicephalus (boophilus) microplus metalloprotease.ticks have serious impacts on animal and human health, causing significant economic losses in cattle breeding. besides damage due to the hematophagous behavior, they transmit several pathogens. low cost and environmental safety have made vaccines a promising alternative control method against tick infestation. metalloproteases (mps) have been shown to be essential for diverse biological functions in hematophagous organisms, inhibiting blood clotting, degrading extracellular matrix proteins, and ...201525480468
a spatially explicit metapopulation model and cattle trade analysis suggests key determinants for the recurrent circulation of rift valley fever virus in a pilot area of madagascar highlands.rift valley fever (rvf) is a vector-borne zoonotic disease that causes high morbidity and mortality in ruminants. in 2008-2009, a rvf outbreak affected the whole madagascar island, including the anjozorobe district located in madagascar highlands. an entomological survey showed the absence of aedes among the potential rvf virus (rvfv) vector species identified in this area, and an overall low abundance of mosquitoes due to unfavorable climatic conditions during winter. no serological nor virolog ...201425474116
a comparison of three different methodologies for evaluating rhipicephalus (boophilus) microplus susceptibility to topical spray compounds.this study aimed to compare three different methodologies (adult immersion tests, field trials with naturally infected animals, and a stall test using artificially infested cattle) to evaluate the efficacy of two topical formulations that we administered as whole body sprays (15% cypermethrin+30% chlorpyriphos+15% fenthion-colosso(®) fc 30, ouro fino agronegócios; and 60% dichlorvos+20% chlorpyriphos-ectofós(®), vallée saúde animal ltd.), against a susceptible strain of rhipicephalus (boophilus) ...201525468670
species distribution modelling for rhipicephalus microplus (acari: ixodidae) in benin, west africa: comparing datasets and modelling algorithms.rhipicephalus microplus is one of the most widely distributed and economically important ticks, transmitting babesia bigemina, b. bovis and anaplasma marginale. it was recently introduced to west africa on live animals originating from brazil. knowing the precise environmental suitability for the tick would allow veterinary health officials to draft vector control strategies for different regions of the country. to test the performance of modelling algorithms and different sets of environmental ...201525466219
an atypical residue in the pore of varroa destructor gaba-activated rdl receptors affects picrotoxin block and thymol modulation.gaba-activated rdl receptors are the insect equivalent of mammalian gabaa receptors, and play a vital role in neurotransmission and insecticide action. here we clone the pore lining m2 region of the varroa mite rdl receptor and show that it has 4 atypical residues when compared to m2 regions of most other insects, including bees, which are the major host of varroa mites. we create mutant drosophila rdl receptors containing these substitutions and characterise their effects on function. using two ...201425460510
amblyomma americanum tick calreticulin binds c1q but does not inhibit activation of the classical complement cascade.in this study we characterized amblyomma americanum (aam) tick calreticulin (crt) homolog in tick feeding physiology. in nature, different tick species can be found feeding on the same animal host. this suggests that different tick species found feeding on the same host can modulate the same host anti-tick defense pathways to successfully feed. from this perspective it's plausible that different tick species can utilize universally conserved proteins such as crt to regulate and facilitate feedin ...025454607
comparative microarray analyses of adult female midgut tissues from feeding rhipicephalus species.the cattle tick, rhipicephalus microplus, has a debilitating effect on the livestock industry worldwide, owing to its being a vector of the causative agents of bovine babesiosis and anaplasmosis. in south africa, co-infestation with r. microplus and r. decoloratus, a common vector species on local livestock, occurs widely in the northern and eastern parts of the country. an alternative to chemical control methods is sought in the form of a tick vaccine to control these tick species. however, seq ...201525448423
chemical composition and acaricidal activity of the essential oil of baccharis dracunculifolia de candole (1836) and its constituents nerolidol and limonene on larvae and engorged females of rhipicephalus microplus (acari: ixodidae).baccharis dracunculifolia dc (common name "alecrim-do-campo" in brazil) is a plant with widespread distribution in south america that is the botanical origin of green propolis. the aim of this study was to evaluate the chemical composition and acaricidal activity of the essential oil of b. dracunculifolia and its constituents nerolidol and limonene on unengorged larvae and engorged females of rhipicephalus microplus (acari: ixodidae). the essential oil yield was 0.8% of dry mass and the major co ...201525448290
ticks infesting wild and domestic animals and humans of sri lanka with new host records.an island-wide collection of tick species infesting humans, domesticated and wild animals and questing ticks in domestic and peridomestic environments was carried out during 2009-2011. a total of 30,461 ticks were collected from 30 different hosts and free living stages from the ground. the collection consisted of 22 tick species from 30 different hosts recording 12 tick species from humans, 19 from domesticated animals and 21 from wild animals, with a total of 97 new host records. the most comm ...201525445744
key sites for p2x receptor function and multimerization: overview of mutagenesis studies on a structural basis.p2x receptors constitute a seven-member family (p2x1-7) of extracellular atp-gated cation channels of widespread expression. because p2x receptors have been implicated in neurological, inflammatory and cardiovascular diseases, they constitute promising drug targets. since the first p2x cdna sequences became available in 1994, numerous site-directed mutagenesis studies have been conducted to disclose key sites of p2x receptor function and oligomerization. the publication of the 3-a crystal struct ...025439586
seroepidemiologic survey in thailand of coxiella burnetii infection in cattle and chickens and presence in ticks attached to dairy cattle.a seroepidemiologic survey of coxiella burnetii in cattle and chickens in thailand was carried out using indirect fluorescent antibody test. nine of the 130 serum samples from cattle were positive for antibodies against c. burnetii, with antibody titers ranging from 32 to 64. only one of 113 serum samples from chickens was seropositive, with antibody titer of 16. no c. burnetii-specific dna was detected using restriction fragment length polymorphism-nested pcr in spleens of cattle and chickens. ...201425417520
contributions of the hair sheep breed santa ines as a maintenance host for rhipicephalus (boophilus) microplus (acari: ixodidae) in brazil.hair sheep breeds are a new, cost-effective option for the diversification of livestock in the midwest region of brazil. they are grazed extensively with cattle as well as in isolation in small areas. hair sheep breeds are vulnerable to infestation by parasites such as the cattle tick, rhipicephalus microplus, which causes various types of damage and can transmit diseases.201425404176
efficacy of larvicidal activity of green synthesized titanium dioxide nanoparticles using mangifera indica extract against blood-feeding parasites.titanium dioxide nanoparticles (tio2 nps) are considered to be among the best photocatalytic materials due to their long-term thermodynamic stability, strong oxidizing power, and relative non-toxicity. nano-preparations with tio2 nps are currently under investigation as novel treatments for acne vulgaris, recurrent condyloma acuminata, atopic dermatitis, hyperpigmented skin lesions, and other non-dermatologic diseases. the present study was to investigate the acaricidal and larvicidal activity o ...201525403378
phytotherapeutic activity of euphorbia cyparissias extracts on ixodidae (acari) female ticks.given its numerous biologically active components, euphorbiaceae has been found to be a large plant family and polyvalent with quite interesting therapeutic activity that can be studied.201425392580
acaricidal activity of ocimum basilicum and spilanthes acmella against the ectoparasitic tick, rhipicephalus (boophilus) microplus (arachinida: ixodidae).the ectoparasitic tick, rhipicephalus (boophilus) microplus collected at various cattle farms in and around chennai was subjected to treatment of different crude solvent extracts of leaves of ocimum basilicum and spilanthes acmella for acaricidal activity. among various solvent extracts of leaves of o. basilicum and s. acmella used, chloroform extract of o. basilicum at concentrations between 6% and 10% exhibited 70% and 100% mortality of ticks when compared to control. the lc50 and lc90 values ...201425382467
bartonella chomelii is the most frequent species infecting cattle grazing in communal mountain pastures in spain.the presence of bartonella spp. was investigated in domestic ungulates grazing in communal pastures from a mountain area in northern spain, where 18.3% (17/93) of cattle were found to be positive by pcr combined with a reverse line blot (pcr/rlb), whereas sheep (n = 133) or horses (n = 91) were found not to be infected by this pathogen. bartonella infection was significantly associated with age, since older animals showed a higher prevalence than heifers and calves. in contrast to other studies, ...201425381240
selection and validation of reference genes for functional studies in the calliphoridae family.the genera cochliomyia and chrysomya contain both obligate and saprophagous flies, which allows the comparison of different feeding habits between closely related species. among the different strategies for comparing these habits is the use of qpcr to investigate the expression levels of candidate genes involved in feeding behavior. to ensure an accurate measure of the levels of gene expression, it is necessary to normalize the amount of the target gene with the amount of a reference gene having ...201425373149
development of a spatially targeted field sampling technique for the southern cattle tick, rhipicephalus microplus, by mapping white-tailed deer, odocoileus virginianus, habitat in south texas.the objective of our study was to determine whether satellite remote sensed data could be used to identify white-tailed deer, odocoileus virginianus (zimmerman) (artiodactyla: cervidae), habitat and target locations for sampling free-living larvae of the southern cattle tick, rhipicephalus (boophilus) microplus (canestrini) (ixodida: ixodidae) in south texas. two methods for mapping white-tailed deer habitat were used, an object-oriented method to identify closed canopies and waterways for deer ...025368044
genetic diversity of tick-borne rickettsial pathogens; insights gained from distant strains.the ability to capture genetic variation with unprecedented resolution improves our understanding of bacterial populations and their ability to cause disease. the goal of the pathogenomics era is to define genetic diversity that results in disease. despite the economic losses caused by vector-borne bacteria in the order rickettsiales, little is known about the genetic variants responsible for observed phenotypes. the tick-transmitted rickettsial pathogen anaplasma marginale infects cattle in tro ...025364572
antihistamine response: a dynamically refined function at the host-tick interface.ticks counteract host inflammatory responses by secreting proteins from their saliva that compete for histamine binding. among these tick salivary proteins are lipocalins, antiparallel beta-barrel proteins that sequester small molecules. a tick salivary lipocalin has been structurally resolved and experimentally shown to efficiently compete for histamine with its native receptor (e.g., h1 histamine receptor). to date, molecular dynamics simulations focus on protein-protein and protein-ligand int ...201425358914
physiological changes in rhipicephalus microplus (acari: ixodidae) experimentally infected with entomopathogenic fungi.carbohydrate metabolism plays an important role in the physiology and maintenance of energy stores within living organisms. however, when organisms are exposed to adverse physiological conditions, such as during pathogenic infection, these organisms begin to use alternative substrates (proteins and lipids) for energy production. this paper studied the carbohydrate metabolism of rhipicephalus microplus after infection with beauveria bassiana and metarhizium anisopliae. the parameters evaluated we ...201525346195
risk factors for bunyavirus-associated severe fever with thrombocytopenia syndrome, china.severe fever with thrombocytopenia syndrome (sfts) is an emerging disease that is caused by a novel bunyavirus, referred to as sfts virus. during january 2011 to december 2011 we conducted a case-control study in henan, hubei and shandong provinces of china to determine the risk factors for sfts.201425330383
the evolutionary history and spatiotemporal dynamics of the fever, thrombocytopenia and leukocytopenia syndrome virus (ftlsv) in china.in 2007, a novel bunyavirus was found in henan province, china and named fever, thrombocytopenia and leukocytopenia syndrome virus (ftlsv); since then, ftlsv has been found in ticks and animals in many chinese provinces. human-to-human transmission has been documented, indicating that ftlsv should be considered a potential public health threat. determining the historical spread of ftlsv could help curtail its spread and prevent future movement of this virus.201425329580
malathion resistance in rhipicephalus (boophilus) microplus from ludhiana district, punjab.the resistance status of rhipicephalus (boophilus) microplus collected from noorpur bet village of ludhiana district, punjab was evaluated against malathion by adult immersion test. the adult female ticks showed an upward trend in the mortality percentage with increase in drug concentration. the regression graph of probit mortality of ticks plotted against log values of progressively increasing concentrations of malathion was utilized for the determination of slope of mortality (95 % confidence ...201325320479
host resistance in cattle to infestation with the cattle tick rhipicephalus microplus.resistance to rhipicephalus microplus infestation in cattle has many effector mechanisms, each of which is likely to be modulated by complex, interacting factors. some of the mechanisms of host resistance and their modulating factors have been identified and quantified, although much remains to be explained. the variation in resistance to tick infestation is most marked between bos taurus and bos indicus cattle, taurine cattle given the same exposure carrying between five and 10 times as many ti ...201425313455
evaluation and comparison of the potential of two ferritins as anti-tick vaccines against haemaphysalis longicornis.tick control is an essential aspect of controlling the spread of tick-borne diseases affecting humans and animals, but it presently faces several challenges. development of an anti-tick vaccine is aimed at designing cost-effective and environmentally friendly protection against ticks and tick-borne diseases as an alternative to the use of chemical acaricides. a single vaccine from the tick midgut protein bm86 is currently available for field applications, but its efficacy is limited to only some ...201425306467
rhipicephalus (boophilus) microplus aquaporin as an effective vaccine antigen to protect against cattle tick infestations.vaccination as a control method against the cattle tick, rhipicephalus (boophilus) microplus has been practiced since the introduction of two products in the mid-1990s. there is a need for a vaccine that could provide effective control of r. microplus in a more consistent fashion than existing products. during our transcriptome studies of r. microplus, several gene coding regions were discovered to encode proteins with significant amino acid similarity to aquaporins.201425306139
acaricidal activity of five essential oils of ocimum species on rhipicephalus (boophilus) microplus larvae.the aim of this study was to evaluate the acaricidal activity on the cattle tick rhipicephalus (boophilus) microplus of essential oils from three ocimum species. acaricidal activity of five essential oils extracted from ocimum gratissimum l. (three samples), o. urticaefolium roth, and o. canum sims was evaluated on 14- to 21-day-old rhipicephalus microplus tick larvae using larval packet test bioassay. these essential oils were analyzed by gas chromatography (gc) and gas chromatography-mass spec ...201525300420
acaricidal properties of the essential oil from zanthoxylum caribaeum against rhipicephalus microplus.zanthoxylum caribaeum lamarck (rutaceae) is plant species with a variety of medical applications, including insecticidal activity. this study determined the bioacaricidal activity of the essential oil from z. caribaeum leaves against engorged rhipicephalus (boophilus) microplus (canestrini, 1887) females using the adult immersion test. for this purpose, three serial concentrations (5.0, 2.5, and 1.25%, vol:vol, in 1% dimetilsulfoxide) of the essential oil were used. essential oil 5% caused 65% m ...201425276925
rhipicephalus (boophilus) microplus in the western-central region of rio grande do sul, brazil: multiresistant tick.the aim of the present study was to assess the acaricide resistance of tick populations in the western-central region of rio grande do sul (brazil), which has not previously been reported. fifty-four cattle farms were visited and specimens of rhipicephalus (boophilus) microplus were collected and subjected to the adult immersion test, using nine commercial acaricides in the amidine, pyrethroid and organophosphate groups. climatic data, including monthly precipitation, were recorded. the results ...201625271453
multiple mutations in the para-sodium channel gene are associated with pyrethroid resistance in rhipicephalus microplus from the united states and mexico.acaricide resistant rhipicephalus microplus populations have become a major problem for many cattle producing areas of the world. pyrethroid resistance in arthropods is typically associated with mutations in domains i, ii, iii, and iv of voltage-gated sodium channel genes. in r. microplus, known resistance mutations include a domain ii change (c190a) in populations from australia, africa, and south america and a domain iii mutation (t2134a) that only occurs in mexico and the u.s.201425266983
comparative genome analysis of entomopathogenic fungi reveals a complex set of secreted proteins.metarhizium anisopliae is an entomopathogenic fungus used in the biological control of some agricultural insect pests, and efforts are underway to use this fungus in the control of insect-borne human diseases. a large repertoire of proteins must be secreted by m. anisopliae to cope with the various available nutrients as this fungus switches through different lifestyles, i.e., from a saprophytic, to an infectious, to a plant endophytic stage. to further evaluate the predicted secretome of m. ani ...201425263348
artificial feeding of rhipicephalus microplus female ticks with anti calreticulin serum do not influence tick and babesia bigemina acquisition.ticks are obligate haematophagous ectoparasites considered the principal vectors of disease among animals. rhipicephalus microplus and r. annulatus ticks are the most important vectors for babesia bigemina and b. bovis, two of the most important intraerythrocytic protozoan parasites species in cattle, responsible for babesiosis which together with anaplasmosis account for substantial economic losses in the livestock industry worldwide. anti-tick vaccines are a proved alternative to traditional t ...201525262467
nhumirim virus, a novel flavivirus isolated from mosquitoes from the pantanal, brazil.we describe the isolation of a novel flavivirus, isolated from a pool of mosquitoes identified as culex (culex) chidesteri collected in 2010 in the pantanal region of west-central brazil. the virus is herein designated nhumirim virus (nhuv) after the name of the ranch from which the mosquito pool was collected. flavivirus rna was detected by real-time rt-pcr of homogenized mosquitoes and from the corresponding c6/36 culture supernatant. based on full-genome sequencing, the virus isolate was gene ...201425252815
influence of the biotope on the tick infestation of cattle and on the tick-borne pathogen repertoire of cattle ticks in ethiopia.the majority of vector-borne infections occur in the tropics, including africa, but molecular eco-epidemiological studies are seldom reported from these regions. in particular, most previously published data on ticks in ethiopia focus on species distribution, and only a few molecular studies on the occurrence of tick-borne pathogens or on ecological factors influencing these. the present study was undertaken to evaluate, if ticks collected from cattle in different ethiopian biotopes harbour (had ...201425248165
first molecular detection of rickettsia africae in ticks from the union of the comoros.rickettsia africae is the agent of african tick bite fever, a disease transmitted by ticks in sub-saharan africa. in union of the comoros, a recent study reported the presence of a rickettsia africae vector but no information has been provided on the circulation of the pathogenic agent in this country.201425245895
bioinformatic analyses of male and female amblyomma americanum tick expressed serine protease inhibitors (serpins).serine protease inhibitors (serpins) are a diverse family of proteins that is conserved across taxa. the diversity of amblyomma americanum serpins (aas) is far more complex than previously thought as revealed by discovery of 57 and 33 aas transcripts that are respectively expressed in male and female a. americanum ticks, with 30 found in both. while distinct reproductively, both male and female metastriate ticks, such as a. americanum, require a blood meal. thus, 30 aas sequences found in both m ...201425238688
a list of the 70 species of australian ticks; diagnostic guides to and species accounts of ixodes holocyclus (paralysis tick), ixodes cornuatus (southern paralysis tick) and rhipicephalus australis (australian cattle tick); and consideration of the place of australia in the evolution of ticks with comments on four controversial ideas.seventy species of ticks are known from australia: 14 soft ticks (family argasidae) and 56 hard ticks (family ixodidae). sixteen of the 70 ticks in australia may feed on humans and domestic animals (barker and walker 2014). the other 54 species of ticks in australia feed only on wild mammals, reptiles and birds. at least 12 of the species of ticks in australian also occur in papua new guinea. we use an image-matching system much like the image-matching systems of field guides to birds and flower ...201425236960
genomic analyses and transcriptional profiles of the glycoside hydrolase family 18 genes of the entomopathogenic fungus metarhizium anisopliae.fungal chitin metabolism involves diverse processes such as metabolically active cell wall maintenance, basic nutrition, and different aspects of virulence. chitinases are enzymes belonging to the glycoside hydrolase family 18 (gh18) and 19 (gh19) and are responsible for the hydrolysis of β-1,4-linkages in chitin. this linear homopolymer of n-acetyl-β-d-glucosamine is an essential constituent of fungal cell walls and arthropod exoskeletons. several chitinases have been directly implicated in str ...201425232743
influence of ph control in the formation of inclusion bodies during production of recombinant sphingomyelinase-d in escherichia coli.inclusion bodies (ibs) are aggregated proteins that form clusters when protein is overexpressed in heterologous expression systems. ibs have been considered as non-usable proteins, but recently they are being used as functional materials, catalytic particles, drug delivery agents, immunogenic structures, and as a raw material in recombinant therapeutic protein purification. however, few studies have been made to understand how culture conditions affect the protein aggregation and the physicochem ...201425213001
impact of humidity on clustered tick eggs.eclosion of larvae from clustered tick eggs (1, 5, 10, 15, 20, 25, 50, and ±1,000 eggs) of rhipicephalus sanguineus (rs), rhipicephalus microplus (rm), amblyomma cajennense (ac), and dermacentor nitens (dn) was investigated at 43, 75, and > 98% relative humidity (rh) and 25 °c. susceptibility of eggs to dehydration varied among tick species (rs < rm < ac and dn) and the number of clustered eggs but even singularized eggs of all tested species matured, and larvae eclosed at rh > 98%. a minimum of ...201425199556
acaricidal properties of the formulations based on essential oils from cymbopogon winterianus and syzygium aromaticum plants.the cattle tick, rhipicephalus (boophilus) microplus, has caused serious harm to livestock raising in brazil, considering the costs of controlling it, loss of revenue due to smaller production of milk and meat, and damage to leather, in addition to transmitting diseases. the use of medicinal plants is considered an alternative to the recurring resistance to chemicals. due to the need for efficient alternatives with less environmental impact, this study aimed to develop contact formulations with ...201425199555
dual silencing of long and short amblyomma americanum acidic chitinase forms weakens the tick cement cone stability.this study demonstrates that amblyomma americanum (aam) constitutively and ubiquitously expresses the long (l) and short (s) putative acidic chitinases (ach) that are distinguished by a 210 base pair (bp) deletion in aamach-s. full-length aamach-l and aamach-s cdna are 1959 and 1718 bp long, containing 1332 and 1104 bp open reading frames that code for 443 and 367 amino acid residues proteins with the former predicted to be extracellular and the latter intracellular. both aamach-l and aamach-s m ...201425189365
parasiticide resistance in flies, lice and ticks in new zealand and australia: mechanisms, prevalence and prevention.this review outlines the history of parasiticide resistance in the principal ectoparasites of sheep and cattle in new zealand and australia, blowflies, buffalo fly (haematobia irritans exigua), sheep biting louse (bovicola ovis) and cattle ticks, and discusses recent changes in their response to insecticides and acaricides. mechanisms of resistance and evaluation methods are described, with ways in which insecticide resistance can be avoided or ameliorated also discussed. resistance in sheep blo ...201525185062
survey on cattle ticks in nur, north of iran.to survey the prevalence of cattle ticks in nur county and prepare a list of tick fauna in this district.201425182439
molecular cloning and characterization of a glycine-like receptor gene from the cattle tick rhipicephalus (boophilus) microplus (acari: ixodidae).the cattle tick rhipicephalus (boophilus) microplus is the most economically important ectoparasite affecting the cattle industry in tropical and subtropical areas around the world. the principal method of tick control has relied mainly on the use of chemical acaricides, including ivermectin; however, cattle tick populations resistant to ivermectin have recently been reported in brazil, mexico, and uruguay. currently, the molecular basis for ivermectin susceptibility and resistance are not well ...201425174962
tick passage results in enhanced attenuation of babesia bovis.serial blood passage of virulent babesia bovis in splenectomized cattle results in attenuated derivatives that do not cause neurologic disease. tick transmissibility can be lost with attenuation, but when retained, attenuated b. bovis can revert to virulence following tick passage. this study provides data showing that tick passage of the partially attenuated b. bovis t2bo derivative strain further decreased virulence compared with intravenous inoculation of the same strain in infected animals. ...201425114111
microarray analysis of tick-infested skin in resistant and susceptible cattle confirms the role of inflammatory pathways in immune activation and larval rejection.tick bites promote activation of an inflammatory process that is influenced by bovine genetic composition and its history of previous exposure. taurine and indicine breeds are known to differ on its immune response development against rhipicephalus microplus. nevertheless, further investigation about the complex molecular pathways involved in the development of immune response to tick infestation in cattle presenting the same genetic background is mandatory. the aim of this work was to access th ...201425108850
genetic parameters and investigation of genotype × environment interactions in nellore × hereford crossbred for resistance to cattle ticks in different regions of brazil.data from 6,156 nellore × hereford crossbred cattle, distributed in 18 herds located in the brazilian states of mato grosso do sul (ms), são paulo (sp), paraná (pr) and rio grande do sul (rs), were analysed in order to investigate genetic variation for resistance to the cattle tick through the estimation of variance components and genetic parameters for counting ticks (rhipicephalus (boophilus) microplus) in natural infestation, and also a possible genotype × environment interaction. the tick co ...201525108748
commercial formulation of metarhizium anisopliae for the control of rhipicephalus microplus in a pen study.the present study evaluated, for the first time, the effect of the commercial formulation metarril(®) sp organic of metarhizium anisopliae plus 10% mineral oil to control rhipicephalus microplus in a pen study. three groups were formed with six animals each: the first group was exposed to metarril(®) plus 10% mineral oil and 1% tween 80; the second group was exposed to sterile distilled water, mineral oil and tween 80 (oil control group); and the third group received no treatment (control group) ...201425086495
in vitro and in vivo acaricide action of juvenoid analogs produced from the chemical modification of cymbopogon spp. and corymbia citriodora essential oil on the cattle tick rhipicephalus (boophilus) microplus.the present study aimed to evaluate the acaricidal action of the chemically modified essential oil of cymbopogon spp. and corymbia citriodora on rhipicephalus (boophilus) microplus. citronellal was converted into n-butylcitronellylamine and in n-prop-2-inylcitronellylamine, analogs of juvenoids, by reductive amination using butylamine (n1 to n3) and propargylamine (n4 to n7). in vitro assays included the adult immersion, and larval packet tests. engorged females were weighed in groups of 10 and ...201425085774
transcriptome of the female synganglion of the black-legged tick ixodes scapularis (acari: ixodidae) with comparison between illumina and 454 systems.illumina and 454 pyrosequencing were used to characterize genes from the synganglion of female ixodes scapularis. go term searching success for biological processes was similar for samples sequenced by both methods. however, for molecular processes, it was more successful for the illumina samples than for 454 samples. functional assignments of transcripts predicting neuropeptides, neuropeptide receptors, neurotransmitter receptors and other genes of interest was done, supported by strong e-value ...201425075967
understanding anaplasmataceae pathogenesis using "omics" approaches.this paper examines how "omics" approaches improve our understanding of anaplasmataceae pathogenesis, through a global and integrative strategy to identify genes and proteins involved in biochemical pathways key for pathogen-host-vector interactions. the anaplasmataceae family comprises obligate intracellular bacteria mainly transmitted by arthropods. these bacteria are responsible for major human and animal endemic and emerging infectious diseases with important economic and public health impac ...201425072029
rmcystatin3, a cysteine protease inhibitor from rhipicephalus microplus hemocytes involved in immune response.the rhipicephalus microplus tick is responsible for losses in the livestock production estimated in 2 billions usd. despite its economical importance the knowledge in tick's physiology is sparse. in order to contribute to this scenario we describe the characterization of a cysteine proteinase inhibitor named rmcystatin-3. purified recombinant rmcystatin-3 was able to inhibit cathepsin l (ki = 2.5 nm), bmcl1 (ki = 1.8 nm) and cathepsin b (ki = 136 nm). western blot and quantitative pcr analysis r ...201425064361
severe fever with thrombocytopenia syndrome virus in ticks collected from humans, south korea, 2013.we investigated the infection rate for severe fever with thrombocytopenia syndrome virus (sftsv) among ticks collected from humans during may-october 2013 in south korea. haemaphysalis longicornis ticks have been considered the sftsv vector. however, we detected the virus in h. longicornis, amblyomma testudinarium, and ixodes nipponensis ticks, indicating additional potential sftsv vectors.025061851
irspi, a tick serine protease inhibitor involved in tick feeding and bartonella henselae infection.ixodes ricinus is the most widespread and abundant tick in europe, frequently bites humans, and is the vector of several pathogens including those responsible for lyme disease, tick-borne encephalitis, anaplasmosis, babesiosis and bartonellosis. these tick-borne pathogens are transmitted to vertebrate hosts via tick saliva during blood feeding, and tick salivary gland (sg) factors are likely implicated in transmission. in order to identify such tick factors, we characterized the transcriptome of ...201425057911
effect of various acaricides on hatchability of eggs of rhipicephalus (boophilus) microplus.the effect of commonly used acaricides (amitraz, cypermethrin, deltamethrin, fenvalerate, and flumethrin) on the eggs of rhipicephalus (boophilus) microplus collected from ludhiana, punjab, was evaluated by egg hatch assay (eha). the regression graph of probit hatchability and per cent inhibition of hatching (ih%) of eggs was plotted against log values of concentration of various acaricides. all concentrations of flumethrin and amitraz caused complete inhibition of hatching, whereas a hatchabili ...201425057486
reassessment of the potential economic impact of cattle parasites in brazil.the profitability of livestock activities can be diminished significantly by the effects of parasites. economic losses caused by cattle parasites in brazil were estimated on an annual basis, considering the total number of animals at risk and the potential detrimental effects of parasitism on cattle productivity. estimates in u.s. dollars (usd) were based on reported yield losses among untreated animals and reflected some of the effects of parasitic diseases. relevant parasites that affect cattl ...201425054492
rhipicephalus (boophilus) microplus resistant to acaricides and ivermectin in cattle farms of mexico.ticks and the diseases they transmit cause great economic losses to livestock in tropical countries. non-chemical control alternatives include the use of resistant cattle breeds, biological control and vaccines. however, the most widely used method is the application of different chemical classes of acaricides and macrocyclic lactones. populations of the cattle tick, rhipicephalus (boophilus) microplus, resistant to organophosphates (op), synthetic pyrethroids (sp), amitraz and fipronil have bee ...201425054487
Displaying items 401 - 500 of 2301