Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| measurement of serum levels of eosinophil cationic protein in the diagnosis of acute gastrointestinal anisakiasis. | thirty-two patients with abdominal pain and/or intestinal pseudo-obstruction who had consumed raw or undercooked fish in the previous 72 h, were included in a study of anisakiasis, a parasitation of the human gastrointestinal tract by third stage anisakis simplex larvae. skin prick test (spt) against a. simplex were positive in all the patients. high median eosinophil cationic protein (ecp) serum concentrations (> 15 mg/l) at day 0 with normal serum levels at day 30 and a rise of median total an ... | 2003 | 12848764 |
| a case of acute gastric anisakiasis provoking severe clinical problems by multiple infection. | acute gastric anisakiasis with multiple anisakid larvae infection is reported. a 68-year-old woman residing in busan, korea, had epigastric pain with severe vomiting about 5 hours after eating raw anchovies. four nematode larvae penetrating the gastric mucosae in the great curvature of the middle body and fundus were found and removed during gastro-endoscopic examination. another one thread-like moving larva was found in the great curvature of upper body on the following day. on the basis of the ... | 2003 | 12815320 |
| [acute abdomen due to anisakis simplex]. | 2003 | 12809594 | |
| [gastrointestinal anisakiasis. study of a series of 25 patients]. | infection with the parasite anisakis simplex is common in japan and northern european countries. the number of reported cases in spain has increased since the first description in 1991. the aim of the present study was to evaluate the incidence, clinical patterns, histopathological lesions, treatment, and outcome of anisakis simplex infection in our environment. | 2003 | 12809570 |
| human anisakiasis: diversity in antibody response profiles to the changing antigens in larval excretions/secretions. | anisakiasis is an infectious parasitic disease contracted by eating third stage larvae of anisakis simplex (l3) carried by marine fishes. human anisakiasis was researched for specific igg with l3 excretory secretory products (esp). l3esp were prepared by daily harvesting of culture supernatant from day 2 to day 5, using two kinds culture media of rpmi-1640 and phosphate buffered saline (pbs). when the sera from persons diagnosed with anisakiasis by means of endoscopy were analyzed using indirect ... | 2003 | 12753432 |
| [risk of sensitization to anisakis simplex among the members of the families of patients with anisakiasis]. | infection by anisakid nematodes is associated with consumption of raw fish. the aim of this paper was to determine the prevalence of sensitization among members of families of patients with anisakiasis. individuals and methods: eleven asymptomatic members of families of patients diagnosed with anisakiasis were studied by means of ige-immunoblotting. the frequency of sensitization was compared with the prevalence in a normal population from the same geographic area. | 2003 | 12681218 |
| metazoan parasites and food composition of juvenile etmopterus spinax (l., 1758) (dalatiidae, squaliformes) from the norwegian deep. | a total of 37 juvenile etmopterus spinax from the norwegian deep were examined for stomach contents and metazoan ecto- and endoparasites. these squaliform elasmobranchs were caught by benthopelagic net in may 2001 at a depth of 194-214 m. the euphausiid meganyctiphanes norvegica and the teleost maurolicus muelleri were the principal prey organisms. with increasing total length of e. spinax, the frequency of meganyctiphanes norvegica prey decreased and that of m. muelleri increased. seven differe ... | 2003 | 12632160 |
| ultrastructural localization of ani s 1, a major allergen from the fish parasite anisakis simplex. | no data about the nature or function of the first major anisakis simplex allergen, named ani s 1, is available. the aim of this study was to investigate the ultrastructural localization of this protein, to obtain further information about it. ani s 1 was detected in secretory granules of the excretory gland and occasionally lining the main excretory canal. our results suggest that ani s 1 could be a secretory product and could have an enzymatic function related to mechanisms of infection. | 2003 | 12632151 |
| growth of whaleworm (anisakis simplex, nematodes, ascaridoidea, anisakidae) third-stage larvae in paratenic fish hosts. | the growth of anisakis simplex l3 larvae in the three paratenic hosts saithe ( pollachius virens), cod (gadus morhua) and redfish (sebastes marinus) was studied based on material collected off the island of vega on the west coast of norway over a period of 1 year. in all three fish species, a positive correlation between the length of larvae and the age of the fish was shown. the number of large larvae, here defined as l3>28 mm, increases with increasing age of the host. these findings clearly i ... | 2003 | 12632142 |
| digestive anisakiasis: clinical manifestations and diagnosis according to localization. | digestive anisakiasis is a parasitic disease whose clinical manifestations depend on the effect of anisakis simplex on the digestive tract wall. larvae are acquired by eating raw or poorly cooked fish. it is estimated that this entity is currently under-diagnosed, although publications are becoming increasingly common. we analyse our series of digestive anisakiasis checking localization and studying its relationship with symptoms and method of diagnosis. | 2002 | 12486851 |
| dietary recommendations for patients allergic to anisakis simplex. | anisakis simplex, a fish parasite, causes allergic urticaria, angioedema and anaphylactic shock through an ige-mediated hypersensitivity mechanism. consensus on the dietary recommendations that should be given to allergic patients is lacking. our objective was to evaluate the usefulness of different types of diets in preventing further reactions in patients allergic to a. simplex. | 2002 | 12464163 |
| the nematode anisakis simplex in american shad (alosa sapidissima) in two oregon rivers. | this paper represents the first report of the nematode anisakis simplex in the american shad (alosa sapidissima) in its introduced range in the american pacific northwest. all the adult shad sampled from spawning populations in the willamette (n = 9) and umpqua (n = 12) rivers were infected with a. simplex with intensities ranging from 6 to 89 worms per fish. this preliminary investigation contrasts sharply with previous studies in the native range of american shad and confirms that this fish ma ... | 2002 | 12435154 |
| [allergy to anisakis simplex]. | anisakis simplex is a nematode which infects marine fish. it requires marine mammals for its development. the larvae are found in fishes, crustaceans and cephalopods which are intermediate hosts. the parasite can be ingested by man -mainly with raw fishes- and induces an infestation called anisakiasis or anisakidosis with digestive tract symptoms. since 1990, we have known that the parasite can also induce allergic symptoms such urticaria. | 2002 | 12389449 |
| type i and type iv sensitization to anisakis simplex in 2 patients with hand eczema. | 2002 | 12190630 | |
| specific ige determination to ani s 1, a major allergen from anisakis simplex, is a useful tool for diagnosis. | the most serious limitation of the serodiagnosis of parasitoses is the occurrence of cross-reactions. | 2002 | 12141725 |
| acute allergic reactions to anisakis simplex after ingestion of anchovies. | anisakis simplex is a seafish nematode, which is responsible for the well-known human infection (anisakiasis) and can induce ige-mediated reactions. ige sensitization to anisakis simplex can be frequent in particular countries and should be suspected in patients with acute allergic symptoms after ingestion of fish. the etiological role of anisakis simplex was evaluated in 49 adult subjects with acute allergic symptoms after ingestion of anchovies. serum-specific ige and prick tests to anchovy we ... | 2002 | 12125940 |
| conjunctival provocation tests in the diagnosis of anisakis simplex hypersensitivity. | the prevalence of positive skin tests to anisakis simplex is high compared with the low incidence of true gastroallergic anisakiasis. there is little information about the prevalence of positive conjunctival tests in a. simplex-sensitized individuals. this study assesses the predictive value of conjunctival provocation test in two groups of a. simplex-sensitized subjects and negative controls. | 2002 | 12109527 |
| allozyme and pcr-rflp markers in anisakid nematodes, aethiological agents of human anisakidosis. | in the framework of the researches granted by murst cofin97, studies on anisakid nematodes, aethiological agents of human anisakidosis, were carried out. the project was aimed to implement the knowledge on the systematics, genetics, ecology and epizootiology of species of the genera anisakis and pseudoterranova by applying genetic markers obtained from multilocus allozyme electrophoresis and from pcr-based techniques. the results obtained by allozyme studies allowed to extend the geographic dist ... | 2001 | 12078474 |
| gastro-allergic anisakiasis as a consequence of simultaneous primary and secondary immune response. | gastro-allergic anisakiasis has been reported as an entity in which an acute parasitism by anisakis simplex is accompanied by an immunoglobulin (ig)e-mediated systemic allergic reaction. serum samples were obtained from 24 patients within 24 h after the onset of symptoms (day 0) and after 1 month (day 30) and in 13 patients after 6 months. total ige was assessed by the imx method. specific ige was assessed by cap-feia. specific igm, igg, igg4 and iga antibodies were assessed by enzyme-linked imm ... | 2002 | 12060318 |
| effects of ivermectin and albendazole against anisakis simplex in vitro and in guinea pigs. | the activity of ivermectin and albendazole against larval anisakis simplex was tested in vitro and in experimentally infected guinea pigs. before drug exposure the medium for half of the larvae was adjusted to ph 2.0 with 1 n hcl, whereas the other half was held at ph 7.0. to these solutions, ivermectin was added to full concentrations of 1, 2, 5, 10, 50, 100, or 200 microg/ml, and for albendazole, 300, 400, and 500 microg/ml. animals from group i were given 0.1 ml of 1% (3.3 mg/kg) ivermectin, ... | 2002 | 12054018 |
| identification of anisakid nematodes from the southern baltic sea using pcr-based methods. | four common species of baltic anisakids anisakis simplex, hysterothylacium auctum, contracaecum osculatum from fish (the last one also from the grey seal) and c. rudolphii from cormorants were examined using pcr-rflp technique for working out the method allowing their identification and differentiation. a fragment of nuclear dna containing intergenic regions (its-1 and its-2) of ribosomal dna together with adjacent sequences of genes coding 18s and 28s rrna (in total; approximately 1500 bp) was ... | 2002 | 12030761 |
| evaluation by elisa of anisakis simplex larval antigen purified by affinity chromatography. | in order to improve the specificity and sensitivity of the techniques for the human anisakidosis diagnosis, a method of affinity chromatography for the purification of species-specific antigens from anisakis simplex third-stage larvae (l3) has been developed. new zealand rabbits were immunized with a. simplex or ascaris suum antigens or inoculated with toxocara canis embryonated eggs. the igg specific antibodies were isolated by means of protein a-sepharose cl-4b beads columns. igg anti-a. simpl ... | 2002 | 12016451 |
| immunoglobulins anti-anisakis simplex in patients with gastrointestinal diseases. | the nematode anisakis simplex causes anisakidiasis, a disease that often mimics other gastrointestinal diseases. patients with digestive haemorrhaging, crohn's disease, digestive cancer and appendicitis were analysed for antibodies to a. simplex. antibody detection was carried out by enzyme-linked immunosorbent assay (elisa) and immunoblotting using crude extract (ce) antigen and excretory-secretory (es) products. total immunoglobulin (igs), igg, igm, iga and ige were studied. the highest percen ... | 2002 | 12015825 |
| uterine adenocarcinoma with generalised metastasis in a bottlenose dolphin tursiops truncatus from northern patagonia, argentina. | an endometrial adenocarcinoma with areas of squamous differentiation and generalised metastasis was observed in a bottlenose dolphin tursiops truncatus stranded in northern patagonia in july 1997. this is the second report of a uterine adenocarcinoma in a free-living cetacean and the first in a delphinidae. this neoplasm likely compromised reproduction for several years. in addition, the dolphin presented tattoo-like skin lesions and its digestive tract was infested by anisakis simplex, pseudote ... | 2002 | 12005238 |
| do only live larvae cause anisakis simplex sensitization? | 2002 | 11991288 | |
| [anisakis simplex and immediate hypersensitivity reactions]. | anisakis simplex is one of the nematode worms that parasitize sea mammals. when its larvae are accidentally ingested by humans, they can infect the host, resulting in anisakiasis manifested by digestive symptoms, or they may cause an allergic reaction, which in some cases may be severe. | 2002 | 11988686 |
| anisakis simplex: dangerous--dead and alive? | the risk of infection with anisakis simplex and related parasites of fish has been recognized for some time, but it is now emerging that ingestion of material from dead parasites in food is also potentially dangerous. the resulting allergic reactions range from rapid onset and potentially lethal anaphylactic reactions to chronic, debilitating conditions. this review discusses the problems and clinical implications associated with a. simplex, other related conditions, and the way in which disease ... | 2002 | 11850010 |
| anisakis simplex larva killed by high-hydrostatic-pressure processing. | anisakis simplex is a common nematode parasite present in many marine fish, including finfish and squid. it can pose a public health problem if it is not destroyed during food processing. anisakis larvae were isolated from fish tissue, and their survival of high-pressure treatments in distilled water and physiological isotonic solution was assayed. treatment at a pressure of 200 mpa for 10 min at a temperature between 0 and 15 degrees c kills all anisakis larvae, with a lack of motility being us ... | 2002 | 11848572 |
| interleukin-4 production in balb/c mice immunized with anisakis simplex. | we investigated the interleukin (il-4) levels in balb/c mice immunized with anisakis extract in single or multiple doses and in mice orally infected with a larva. from animals immunized maximum responses were obtained with the multiple doses with an only il-4 peak. conversely, in the mice inoculated with a larva per os, the il-4 levels showed two peaks of different rates. | 2001 | 11685265 |
| natural antibodies to nematode biotinyl-enzymes in human sera. | biotinyl-enzymes are conservative molecules present in helminths, as well as in other animals, bacteria and plants. they have recently been found to be antigenic in mice, and a potential source of cross-reactivity among helminths. this study investigated the presence in human sera of antibodies reactive with biotinyl-enzymes from the nematodes anisakis simplex, toxocara canis and ascaris suum. biotinyl-enzymes from all these nematodes were recognized by igg1 antibodies in sera from healthy subje ... | 2001 | 11599787 |
| in vitro cultivation of anisakis simplex: pepsin increases survival and moulting from fourth larval to adult stage. | this paper describes the in vitro cultivation of the 3rd-larval stage (l3) of anisakis simplex to adulthood in a much simpler and easier to prepare medium than those described to date. the adult males obtained are between 3.8 and 6.5 cm long and the females between 4.5 and 8.0 cm. some individually cultivated females laid eggs which had an average size of 44.4 x 50.5 microm. the culture conditions were as follows: medium rpmi-1640 supplemented with 20% heat-inactivated fetal bovine serum and 1% ... | 2001 | 11578092 |
| serum cd23 is not altered in gastroallergic anisakiasis, but correlates with the production of specific ige and the amount of polyclonal stimulation. | previous studies have shown elevated serum levels of the cytokines il-4 and scd23 in atopic patients and parasitic disease. gastroallergic anisakiasis is an acute parasitic disease, accompanied by ige-mediated clinical symptoms and an important increase of specific and total ige. | 2001 | 11576082 |
| purification of anisakis simplex antigen by affinity chromatography. | in order to improve the specificity and sensitivity of the techniques for the diagnosis of human anisakidosis, a method of affinity chromatography for the purification of species-specific antigens from anisakis simplex third-stage larvae (l3) has been developed. new zealand rabbits were immunized with a. simplex or ascaris suum antigens or inoculated with toxocara canis embryonated eggs. the igg-specific antibodies were isolated by means of protein a-sepharose cl-4b bead columns. igg anti-anisak ... | 2001 | 11570559 |
| possible presence of common tyvelose-containing glycans in trichinella l1 larvae and embryonated eggs of several nematodes. | a monoclonal antibody (mab us4) recognising an epitope containing tyvelose within the t. spiralis l-1 muscle larvae (tsl-1) antigens was tested in western-blot against various antigenic preparations from different stages of the following nematodes: t. spiralis (l1, adult), t. muris (egg, l1, l3, adult), ascaris suum (egg, adult), toxocara canis (egg, adult), anisakis simplex (l3) and haemochus contortus (egg). positive reaction was present in antigen preparations from l1 larvae of t. spiralis an ... | 2001 | 11484333 |
| fitness of the marine parasitic nematode anisakis simplex s. str. in temperate waters of the ne atlantic. | in temperate waters of the ne atlantic, third-stage larvae of anisakis simplex sensu stricto collected from 3 paratenic host species were identified by restriction fragment length polymorphisms. the condition of wild larval infrapopulations was assessed by examining morphometric and growth characteristics. the differentiation patterns and the excretory/secretory products of larvae grown in vitro were also examined. an extensive morphometric, growth and differentiation variability was found betwe ... | 2001 | 11463100 |
| element concentration variability in the whaleworm anisakis simplex s.l. | concentrations of the elements c, n, fe, na, k, ca and mg were analysed by chromatography and atomic absorption spectrometry in the whaleworm anisakis simplex sensu lato. nearly all mean inorganic element concentrations measured were higher in adult worms than in larval forms. similarly, adults contained significantly higher nitrogen (i.e. lower c/n ratio values) than either eggs or larvae. evidence for the significance of the parasitic life cycle stage and the systematic position of their hosts ... | 2001 | 11438433 |
| freezing protects against allergy to anisakis simplex. | allergic reactions to anisakis simplex have been described following ingestion of fish and were thought to be possible even if seafood is frozen or well-cooked. | 2001 | 11436972 |
| evidence of infectious diseases in harbour porpoises (phocoena phocoena) hunted in the waters of greenland and by-caught in the german north sea and baltic sea. | the pathological, microbiological and serological findings in harbour porpoises hunted in greenlandic waters were compared with the findings in animals accidentally caught in fishing gear in the german north sea and baltic sea. the body condition of the greenlandic animals was good, whereas nine of 23 german harbour porpoises were moderately to markedly emaciated. both groups were infested with parasites. in the greenlandic animals parasitism of the aural peribullar cavity with stenurus minor, o ... | 2001 | 11430682 |
| anisakis simplex-sensitized patients: should fish be excluded from their diet? | anisakis simplex (a.s.) allergy is an emerging disease. the third-stage larvae of this nematode are a source of hidden allergens in fish. there are no clear guidelines concerning dietary restrictions for patients with serum-specific ige to this parasite. | 2001 | 11428742 |
| prevalence of and risk factors for ige sensitization to anisakis simplex in a spanish population. | the number of allergic reactions to a. simplex reported in spain has increased dramatically in the last decade. nevertheless, there have been no studies of the prevalence of and possible risk factors for ige sensitization to this parasite, possibly because suitably specific diagnostic methods have only recently become available. the objective was to investigate the prevalence of and risk factors for ige sensitization to a. simplex in galicia, a region of northwestern spain with a population of a ... | 2001 | 11421926 |
| allergenic cross-reactivity between the nematode anisakis simplex and the dust mites acarus siro, lepidoglyphus destructor, tyrophagus putrescentiae, and dermatophagoides pteronyssinus. | the nematode anisakis simplex is a common parasite on fish and other seafood. it is considered to be a food allergen and to induce ige-mediated reactions. allergenic cross-reactivity between a. simplex and other nematodes has been reported, as has cross-reactivity with arthropods: red mosquito larvae and german cockroach. we have here studied the allergenic relationship between a. simplex and four different dust-mite species. | 2001 | 11421925 |
| characterization of two monoclonal antibodies raised in btk(xid) mice that recognize phosphorylcholine-bearing antigens from trichinella and other helminths. | this study investigated the binding properties of two monoclonal antibodies (mabs us1 and us2) raised in (cba/n x balb/c)f1 (nbf1) btk(xid) male mice. both mabs show unusual specificity for phosphorylcholine (pc)-containing tsl4 antigens of trichinella. specifically, and in contrast to mabs raised in normal mice, us1 and us2 mabs do not bind to artificial pc-protein conjugates and are not inhibited by either free pc or nppc, although us2 was partially inhibited by nppc at high concentration (10( ... | 2001 | 11412384 |
| anisakis infestation in marine fish and cephalopods from galician waters: an updated perspective. | a total of 2,673 fresh specimens of cephalopod and fish representing 35 species were obtained from commercial local fisheries in galician waters (nw spain). they were examined for anisakid nematodes by digestion of the muscle and elution of the viscera and whole body cavity. all larval nematodes recovered were identified by light microscopy and multilocus electrophoresis as belonging to the species anisakis simplex sensu stricto and a. pegreffii. encysted larvae mostly occurred in the viscera bu ... | 2001 | 11411952 |
| cellular immune responses in mice immunized with anisakis simplex larval antigens. | cellular immune responses to anisakis simplex l3 antigens were investigated in balb/c mice injected subcutaneously with a homologous crude extract (ce). popliteal lymph nodes (pln) were found to be increased in size and weight after a. simplex ce footpad injection. the effects of a. simplex ce in vitro proliferation were assayed with non-fractionated pln cells or nylon-wool purified t cells derived from pooled lymph node cells of mice subcutaneously injected with ce. spleen cells from immunized ... | 2001 | 11403383 |
| [anisakiasis: a borderline disorder]. | anisakis simplex is a nematode which can parasitize many different kinds of fish or cephalopods (codfish, salmon, tuna, mackerel, hake, etc). anisakis simplex can cause different diseases in humans. the human being acquires the larvae by eating raw or undercooked seafood. acute anisakiasis is probably caused by an inflammatory and/or allergic response in the digestive tract mucosa with abdominal pain. it can also induce ige-mediated reactions with several clinical manifestations ranging from urt ... | 2001 | 11388051 |
| anaphylaxis due to hypersensitivity to anisakis simplex. | anaphylactic reactions are among the most frequent causes for requests for emergency medical care. it is possible that no clear precipitating factor can be found delaying the onset of adequate treatment and increasing the risk of new episodes of anaphylaxis in the future. hypersensitivity to anisakis simplex, an intestinal parasite found in fish, is an unusual cause of anaphylaxis but it should always be borne in mind in countries where a great deal of fish is consumed, especially if it is eaten ... | 2001 | 11385293 |
| occupational generalised urticaria and allergic airborne asthma due to anisakis simplex. | anisakis simplex (as), a fish and cephalopodes parasite, may cause allergic reactions in humans on eating and/or handling contaminated fish. we present a case of occupational hypersensitivity to as in a woman employed in a frozen-fish factory. she showed both generalised urticarial rash and asthmatic symptoms after work place exposure. all these symptoms immediately disappeared after work place exposure was ceased. the presence of a positive skin prick test and high specific ige values confirmed ... | 2001 | 11358735 |
| ascaris suum-derived products induce human neutrophil activation via a g protein-coupled receptor that interacts with the interleukin-8 receptor pathway. | infection with tissue-migrating helminths is frequently associated with intense granulocyte infiltrations. several host-derived factors are known to mediate granulocyte recruitment to the tissues, but less attention has been paid to how parasite-derived products trigger this process. parasite-derived chemotactic factors which selectively recruit granulocytes have been described, but nothing is known about which cellular receptors respond to these agents. the effect of products from the nematodes ... | 2001 | 11349070 |
| gastrointestinal parasites and prey items from a mass stranding of false killer whales, pseudorca crassidens, in rio grande do sul, southern brazil. | the gastrointestinal tract of 14 false killer whales, 6 males and 8 females, stranded in june 1995 in southern brazil, with total standard lengths from 338 to 507 cm, were analysed for endoparasites and food items. a pregnant female had a male foetus of 77.5 cm. parasites were found in all 14 false killer whales. the nematode anisakis simplex (rudolphi, 1809) was found in the stomach of 57% of the animals and the acanthocephalan bolbosoma capitatum (linstow, 1889) porta, 1908 was present in the ... | 2001 | 11340462 |
| apoptosis-inducing protein, aip, from parasite-infected fish induces apoptosis in mammalian cells by two different molecular mechanisms. | aip (apoptosis-inducing protein) is a protein purified and cloned from chub mackerel infected with the larval nematode, anisakis simplex, which induces apoptosis in various mammalian cells including human tumor cell lines. aip has shown structural and functional homology to l-amino acid oxidase (lao) which oxidizes several l-amino acids including l-lysine and aip-induced apoptosis has been suggested to be mediated by h2o2 generated by lao activity of aip. in this study, we confirmed that recombi ... | 2001 | 11319613 |
| presence of il-4-like molecules in larval excretory-secretory products and crude extracts from anisakis simplex. | acute symptoms of anisakidosis are caused by a type i allergic reaction in the gastrointestinal wall with elevated specific immunoglobulin (ig)e. the purpose of this study was to investigate the presence of interleukin (il)-4 in the larval antigens of anisakis simplex. we detected concentrations of il-4 in pg/ml when larval excretory--secretory products and crude extract from a. simplex were investigated by elisa. specific antibodies were obtained by immunization of rabbits with mouse il-4 and t ... | 2001 | 11309156 |
| [anisakiasis of the stomach--a case report from germany]. | anisakiasis or "herring worm disease" is one of the most important parasitic diseases of the gastrointestinal tract in japan. in 1988 lorenz and warzok published 8 cases of intestinal anisakiasis in eastern germany. in 1988 spehn et al. reported a case of gastric anisakiasis in an aids patient. here, we describe a case of gastric anisakiasis in germany with an impressive serious clinical course. the symptoms--acute abdominal cramps, severe chest pain, diarrhoea, sub-febrile temperatures and leuc ... | 2001 | 11253510 |
| anisakiasis presenting as an obstructive duodenal tumor. a scandinavian case. | a case of chronic anisakiasis presenting as an occluding duodenal tumor is described. significant falls in anisakis simplex-specific serum ige and total ige occurred after resection of the lesion. histopathologic examination showed a chronic eosinophilic granulomatous infiltrate and a tubular sclerotic structure in the antral submucosa consistent with, but not diagnostic for, an a. simplex larva. | 2001 | 11234986 |
| experimental infections of copepods and sticklebacks gasterosteus aculeatus with small ensheathed and large third-stage larvae of anisakis simplex (nematoda, ascaridoidea, anisakidae). | free-swimming ensheathed larvae of anisakis simplex were shown experimentally to be ingested by the copepods oitona similis and acartia tonsa and by the nauplii of barnacles balanus sp. the larvae did not grow in the copepod hemocoel. experimental infections of various malacostracans were unsuccessful. sticklebacks gasterosteus aculeatus are naturally infected with larvae of a. simplex in coastal brackish water. such sticklebacks may have acquired the infection by eating either a crustacean host ... | 2001 | 11199846 |
| larval anisakids parasitizing the blue whiting, micromesistius poutassou, from motril bay in the mediterranean region of southern spain. | a total of 301 blue whiting, micromesistius poutassou risso, 1826, ranging in length from 17 to 28 cm, from motril bay (mediterranean coast, south spain) were examined for anisakid nematodes, as these fish are common items in the spanish mediterranean diet. three anisakid species were morphologically identified with a total prevalence of 10.63%. anisakis simplex s.l. rudolphi, 1809 had a prevalence value of 6.65%, compared with 2.66% for a. physeteris baylis, 1923 and 2.33% for hysterothylacium ... | 2000 | 11138027 |
| [eosinophilic esophagitis associated with recurrent urticaria: is the worm anisakis simplex involved?]. | anisakis simplex, a fish parasite of the nematode family, typically infects marine mammals such as whales, dolphins and seals. human anisakiasis, which is acquired by eating raw or insufficiently heated fish or squid, has gained world-wide importance. infestation with living larvae caused by eating parasitised fish results in acute upper abdominal pain, nausea and vomiting and may be confused with acute abdomen due to appendicitis and other inflammatory abdominal disorders. extraintestinal organ ... | 2000 | 11130147 |
| shared epitope for monoclonal ir162 between anisakis simplex larvae and clonorchis sinensis and cross-reactivity between antigens. | ir162 is a rat monoclonal ige antibody (mab). in the investigation of rat ige production, the antigens recognized by ir162 (ir-ags) were found to be expressed by a variety of helminthic parasites. by western blot analysis, ir162 detected bands in crude extracts of anisakis simplex larvae, clonorchis sinensis, paragonimus westermani, plerocercoids of spirometra mansoni, and toxocara canis. excretory-secretory material from a. simplex larvae also contained a protein recognized by ir162. ir162 mab ... | 2000 | 11128497 |
| a pcr-rflp assay for identification of anisakis simplex from different geographical regions. | anisakis simplex, a nematode from the family anisakidae, is a parasite of fish and mammals. it is a casual agent of a human disease called anisakiosis. we found that the assay based on pcr amplification of the its-1-5.8 s-its-2 fragment of rdna and subsequent restriction fragment length polymorphism, previously described on the basis of a. simplex isolated solely from one geographical region, can be used as a general test for identification of this worm species. the restriction patterns analysed ... | 2000 | 11090264 |
| molecular cloning of paramyosin, a new allergen of anisakis simplex. | anisakis simplex is a fish parasite that, when accidentally ingested by humans, may cause allergic reactions in sensitized individuals. the main objectives of our study were to: (1) construct a cdna expression library of a. simplex; (2) identify clones producing specific ige binding protein antigens, and (3) produce and purify the protein/s codified by the isolated clones produced in escherichia coli. | 2000 | 11060483 |
| predictors of anisakis simplex symptoms. | 2000 | 11030385 | |
| prey, parasites and pathology associated with the mortality of a juvenile gray whale (eschrichtius robustus) stranded along the northern california coast. | an eastern pacific gray whale (eschrichtius robustus) stranded off pelican point, tomales bay, california, usa, was examined for physiological parameters, prey, parasites and associated pathology. the whale was emaciated, and hematological examination revealed an elevation in hematocrit, serum sodium, potassium, electrolyte values and hypoglycemia. parasites recovered included 5 species, 1 ectoparasite (cyamus scammoni), and 4 helminths (anisakis simplex, ogmogaster antarcticus, ogmogaster penta ... | 2000 | 11023250 |
| immunofluorescent localization of intermediate filaments (ifs) in helminths using anti-mammalian ifs monoclonal antibody. | intermediate filaments (ifs) make up the cytoskeleton of most eukaryotic cells. in vertebrates, a number of if proteins have been identified, showing distributions unique to tissue or cell type. information on helminth ifs is limited to some nematode species. to observe immunofluorescent localization of ifs in helminth tissues, we selected a murine hybridoma clone producing igm antibody to multiple types of mammalian if proteins and examined cross-reactivity to helminth proteins. the selected mo ... | 2000 | 10958445 |
| "spring rise" of whaleworm (anisakis simplex; nematoda, ascaridoidea) third-stage larvae in some fish species from norwegian waters. | the seasonal variation in the infection of saithe (pollachius virens), cod (gadus morhua), and redfish (sebastes marinus) from a coastal area of central norway with anisakis simplex third-stage larvae (l3) was studied over a period of 1 year. in all three host species there was an increase in the abundance of the parasite in spring, with a peak appearing in march and april. cod displayed the most distinct seasonal variation, showing a clear abundance peak in april. the abundance peak in april fo ... | 2000 | 10952259 |
| distribution of anisakis simplex in fish caught in the ligurian sea. | a total of 822 individuals of 5 fish species were sampled in the western coastal area of the mediterranean sea and examined for the presence of anisakis simplex larvae in the body cavity, muscle, and viscera. a. simplex mean intensity (mi) and prevalence (pr) values were very high in the common scad (trachurus trachurus; pr 80-100%, mi 19.3-36.8) and the blue scad (t. picturatus; pr 80-100%, mi 18.2-70.7), moderate in the blue whiting (micromesistius poutassou), and low in the red mullet (mullus ... | 2000 | 10935904 |
| usefulness of currently available methods for the diagnosis of anisakis simplex allergy. | serodiagnosis of anisakiosis and anisakis allergy is difficult since many anisakis antigens show cross-reactivity complications. in the present study, we assess the usefulness of the major immunologic methods currently available for the diagnosis of anisakis allergy. | 2000 | 10921461 |
| purification and cloning of an apoptosis-inducing protein derived from fish infected with anisakis simplex, a causative nematode of human anisakiasis. | while investigating the effect of marine products on cell growth, we found that visceral extracts of chub mackerel, an ocean fish, had a powerful and dose-dependent apoptosis-inducing effect on a variety of mammalian tumor cells. this activity was strikingly dependent on infection of the c. mackerel with the larval nematode, anisakis simplex. after purification of the protein responsible for the apoptosis-inducing activity, we cloned the corresponding gene and found it to be a flavoprotein. this ... | 2000 | 10903755 |
| isolation and characterization of a major allergen from the fish parasite anisakis simplex. | ingestion of raw or undercooked fish can lead to infection of human subjects by the fish parasite anisakis simplex, a disease known as anisakiasis or anisakidosis. patients sensitized to this fish parasite show high levels of total and specific ige. cross-reactions seem to explain the fact that specific ige antibodies are also found in a high number of normal subjects, as reported in other parasitoses. | 2000 | 10887322 |
| specific and total ige in patients with recurrent, acute urticaria caused by anisakis simplex. | titres of parasite-specific ige were investigated in 19 patients thought to have recurrent, acute urticaria caused by sensitization to anisakis simplex (dujardin, 1845), before and after they were placed on a fish-free diet. patients with other allergic disease and those being treated with corticosteroids or antihistaminics were excluded. skin-prick tests were carried out with a. simplex extract, and blue- and white-fish extracts. the cap system (pharmacia), a commercial test kit developed for t ... | 2000 | 10884871 |
| trichuris suis: a secretory chymotrypsin/elastase inhibitor with potential as an immunomodulator. | a serine protease inhibitor, termed tscei, was purified from adult-stage trichuris suis by acid precipitation, affinity chromatography (elastase-agarose), and reverse-phase hplc. the molecular weight of tscei was estimated at 6.437 kda by laser desorption mass spectrometry. tscei potently inhibited both chymotrypsin (k(i) = 33.4 pm) and pancreatic elastase (k(i) = 8.32 nm). neutrophil elastase, chymase (mouse mast cell protease-1, mmcp-1), and cathepsin g were also inhibited by tscei, whereas tr ... | 2000 | 10864516 |
| unusual sensitization to anisakis simplex. | 2000 | 10858998 | |
| a double-blind, placebo-controlled oral challenge study with lyophilized larvae and antigen of the fish parasite, anisakis simplex. | the third-stage larvae of anisakis simplex may be a hidden source of allergens in fish. the objective was to determine whether the ingestion of lyophilized a. simplex larvae, or antigen, induces clinical symptoms in a group of a. simplex-sensitized patients. | 2000 | 10858988 |
| gastroallergic anisakiasis: findings in 22 patients. | ingestion of anisakidae larvae in raw seafood may cause anisakiasis. however, despite the high level of consumption of seafood in spain, only a few cases of anisakiasis have been reported until now. anisakis simplex can cause allergic reactions in sensitized patients as a result of its parasitism in the gastrointestinal tract. the purpose of this study was to analyse the clinical findings in 22 patients with gastroallergic anisakiasis. | 2000 | 10847436 |
| cloning and high level expression in escherichia coli of an anisakis simplex tropomyosin isoform. | 2000 | 10838230 | |
| what does anisakis simplex parasitism in gastro-allergic anisakiasis teach us about interpreting specific and total ige values? | gastro-allergic anisakiasis is a mostly transitory clinical entity caused by anisakis simplex (a. simplex) and can be suspected by history and confirmed by fiberoptic gastroscopy and specific ige. | 2000 | 10804096 |
| anisakis simplex: a cause of intestinal pseudo-obstruction. | the ingestion of anisakis simplex larvae may lead to the appearance of gastrointestinal symptoms. however, the number of reported cases of parasitization by anisakis in spain is lower than would be expected in a country with the second-highest fish consumption per inhabitant in the world, particularly since fish is often eaten raw or only slightly cooked. we suggest that the incidence of anisakiasis in spain would be higher if complementary studies were used in all patients suspected of having a ... | 2000 | 10799943 |
| incidence of sensitivity to anisakis simplex in a risk population of fishermen/fishmongers. | anisakis simplex, a fish and cephalopodes parasite, can cause either gastrointestinal symptoms or allergic reactions in humans on eating/handling contaminated fish. | 2000 | 10795653 |
| gastrointestinal infection due to anisakis simplex in southern italy. | the authors present four cases of infection due to anisakis in an area where people are prone to infectious diseases transmitted by raw fish, but in which the presence of this parasite has never been reported. three of four cases were discovered accidently during surgical procedures for co-existing abdominal pathologies. raw fish was apparently not involved in all patients. characteristics of the patients are discussed. | 2000 | 10780346 |
| molecular cloning and characterization of an ige-reactive protein from anisakis simplex: ani s 1. | ingestion of the parasitic nematode anisakis simplex in undercooked fish can cause severe allergic reactions in some individuals. using pooled human sera from sensitized patients we have probed an expression library for a. simplex antigens. one positive clone was found to encode a full length 21 kda protein with strong homology to nematode troponins. the recombinant protein was expressed as a gst-fusion protein and found by immunoblot analysis to react with sera from 20% of allergic patients. th ... | 2000 | 10779602 |
| eosinophilic gastritis due to anisakis: a case report. | the parasite anisakis simplex is a helminth included in the nematode class. when man eats raw or rare fish and cephalopods infested by anisakis larvae, he can acquire the parasitic disease (anisakidosis). the parasite can also originate manifestations of immediate ige mediated hypersensitivity in patients with sensitisation to it. | 2000 | 10757855 |
| digestive haemorrhage in patients with anti-anisakis antibodies. | anisakis simplex parasite causes anisakidosis, a disease that often mimics other gastrointestinal diseases such as peritonitis, appendicitis, crohn's disease, ulcer, etc. patients with digestive haemorrhage caused by ulcers, varices or mallory syndrome were analysed for antibodies to the worm a. simplex. | 2000 | 10750655 |
| [study of 13 cases of anisakiasis in the province of cordoba]. | anisakiasis, or anisakidosis, is a parasitic zoonosis due to the infestation by nematodes of the anisakidae family, mainly by anisakis simplex. notwithstanding its world wide distribution, in our country its appearance is quite recent (1991) with only 19 cases previously reported. we refer 13 cases diagnosed in different hospitals in the province of córdoba, spain, from september 1994 to july 1998 which represents the biggest series described in spain so far. all the patients had a clinical onse ... | 2000 | 10738724 |
| o-glycans as a source of cross-reactivity in determinations of human serum antibodies to anisakis simplex antigens. | anisakis simplex is a seafood-borne parasite that may both infect humans and cause allergy. serodiagnosis of anisakiasis and allergy caused by this nematode is difficult since most anisakis antigens show cross-reactivity problems. | 2000 | 10718853 |
| genetic markers in ribosomal dna for the identification of members of the genus anisakis (nematoda: ascaridoidea) defined by polymerase-chain-reaction-based restriction fragment length polymorphism. | polymerase-chain-reaction-based restriction fragment length polymorphism analysis was performed to establish genetic markers in rdna, for the identification of the three sibling species of the anisakis simplex complex and morphologically differentiated anisakis species, i.e. anisakis physeteris, anisakis schupakovi, anisakis typica and anisakis ziphidarum. different restriction patterns were found between a. simplex sensu stricto and anisakis pegreffii with two of the restriction endonucleases u ... | 2000 | 10704605 |
| association of hypersensitivity to the nematode anisakis simplex with hla class ii drb1*1502-dqb1*0601 haplotype. | anisakiasis as well as allergic and anaphylactoid reactions to anisakis simplex antigens are recently identified clinical entities. they are relatively frequent in countries with habitual raw food consumption, often in the form of large amounts of fish and sea food products. in this communication the relationship between hla class ii alleles and the ige-specific immune response to a. simplex allergen was studied in a defined population in northern spain. individuals with immediate-type anisakis ... | 2000 | 10689122 |
| trichuris suis: a secretory serine protease inhibitor. | a trypsin inhibitor was identified in extracts of adult trichuris suis and culture fluids from 24-h in vitro cultivation of adult parasites. the inhibitor was isolated by acid precipitation, affinity chromatography (trypsin-agarose), and reverse phase hplc as a single polypeptide with a molecular weight estimated at 6.6 kda by laser desorption mass spectrometry. the purified inhibitor associated strongly with trypsin (equilibrium dissociation inhibitory constant (k(j)) of 3.07 nm) and chymotryps ... | 2000 | 10631074 |
| gastroallergic anisakiasis: borderline between food allergy and parasitic disease-clinical and allergologic evaluation of 20 patients with confirmed acute parasitism by anisakis simplex. | human subjects can be parasitized by anisakis simplex by eating raw or undercooked fish. gastric anisakiasis is probably the most frequent clinical entity presenting with severe epigastric pain, vomiting, and diarrhea. in gastroallergic anisakiasis hypersensitivity symptoms predominate. | 2000 | 10629469 |
| acute intestinal anisakiasis in spain: a fourth-stage anisakis simplex larva. | a case of acute intestinal anisakiasis has been reported; a nematode larva being found in the submucosa of the ileum of a woman in jaén (spain). the source of infection was the ingestion of raw engraulis encrasicholus. on the basis of its morphology, the worm has been identified as a fourth-stage larva of anisakis simplex. in spain, this is the ninth report of human anisakiasis and also probably the first case of anisakiasis caused by a fourth-stage larva of a. simplex. | 1999 | 10585662 |
| metazoan parasites of the jack mackerel trachurus murphyi (teleostei, carangidae) in a latitudinal gradient from south america (chile and peru). | the metazoa parasite fauna of the jack mackerel trachurus murphyi nichols 1920, obtained from seven ports from a latitudinal gradient of more than 30 degrees (from paita peru: 05 degrees 10' s to talcahuano, chile 36 degrees 40' s) was quantitatively described. 21 species of metazoan parasites were recorded (monogenea 4, digenea 3, cestoda 5, acanthocephala 2, nematoda 4, copepoda 2, isopoda 1). only four parasites (lernanthropus trachuri, ceratothoa gaudichaudii, corynosoma australis, anisakis ... | 1999 | 10511970 |
| immunopathogenesis of human gastrointestinal infection by anisakis simplex. | anisakis simplex is a parasite of fish, and in the case of human infestation, it should be considered as a possible cause of gastrointestinal disease, especially in countries where raw or undercooked fish is a frequent food. clinical features of anisakiasis may simulate acute abdominal pain, such as that found in patients with gastric ulcers, appendicitis, and crohn's disease. furthermore, many cases of anisakiasis are diagnosed as eosinophilic gastroenteritis, which is a broad term for a specif ... | 1999 | 10482840 |
| expression and characterization of elastase inhibitors from the ascarid nematodes anisakis simplex and ascaris suum. | two elastase inhibitors, aspi-1 and aspi-2, from the parasitic nematode anisakis simplex, have been isolated and characterized. because these inhibitors are similar in size (60 amino acids in length) and primary sequence (52 and 47% identical) to the ascaris suum chymotrypsin/elastase inhibitor-1 (asc/e-1), we suggest that these anisakis elastase inhibitors belong to the same unique class of canonical inhibitors formed by the family of ascaris inhibitors (huang k, strynadka ncj, bernard vd, pean ... | 1999 | 10477178 |
| usefulness of early serial measurement of specific and total immunoglobulin e in the diagnosis of gastro-allergic anisakiasis. | sensitization to anisakis simplex (a. simplex) has been documented to produce severe allergic reactions following ingestion of mainly raw or under-cooked parasitized fish. false positive skin prick tests (spt) or specific ige against this nematode and cross-reactivity restricts diagnosis. gastric anisakiasis and gastro-allergic anisakiasis occur if fish is parasitized by live a. simplex | 1999 | 10469036 |
| human immunoglobulin isotype profiles produced in response to antigens recognized by monoclonal antibodies specific to anisakis simplex. | anisakis simplex is a medically important pathogen which not only causes anisakiasis but may provoke allergy reactions, ranging from mild urticaria to anaphylactic shock. | 1999 | 10457114 |
| asymptomatic gastroduodenal anisakiasis as the cause of anaphylaxis. | a great number of parasites have been reported in fish, but only a few of them are capable of infecting human beings. anisakiasis or anisakidosis is caused by sea nematodes of the genus anisakis, with the main implicated species being anisakis simplex. infection with anisakis causes a wide spectrum of clinical manifestations, ranging from symptoms related to the upper and occasionally lower digestive tract to allergic manifestations, mainly urticaria and anaphylaxis. we report a case of asymptom ... | 1999 | 10445801 |
| survival of anisakis simplex in microwave-processed arrowtooth flounder (atheresthes stomias). | the purpose of this study was to define the relationship between survival and temperature of nematodes of the species anisakis simplex in microwave-processed arrowtooth flounder (atheresthes stomias). ten fillets (each 126 to 467 g, 0.5 to 1.75 cm thick), with an average of five larvae of anisakis simplex per fillet, were processed to target temperatures on high (100%) power using a commercial 700-w microwave oven. fillets were neither covered nor rotated and had a temperature probe inserted to ... | 1999 | 10419216 |
| dietary assessment in five cases of allergic reactions due to gastroallergic anisakiasis. | anisakis simplex can cause allergic reactions in sensitized patients. some of these reactions are related to acute parasitism, as is shown in gastroallergic anisakiasis (anisakiasis with digestive and predominantly allergic symptoms). at present, a nonseafood diet is recommended for all patients with any kind of a. simplex allergy. we wished to confirm the clinical suspicion that patients with allergic symptoms after ingestion of raw or undercooked seafood who are sensitized to a. simplex, and d ... | 1999 | 10380786 |
| analysis of the antigenicity in mice of biotinyl enzymes from anisakis simplex and other nematodes. | we analyzed the antigenicity of biotinyl enzymes contained in somatic extracts from anisakis simplex and other parasite nematodes and show in this report that these molecules are an important source of cross-reactivity problems among these nematodes. cross-reactivity was most pronounced among members of the superfamily ascaridoidea. these results suggest that the presence of biotinyl enzymes in whole somatic extracts of a. simplex and other parasites may make serodiagnostic assays based on this ... | 1999 | 10344536 |
| anti-anisakis antibodies in the clinical course of crohn's disease. | human anisakidosis is a parasitic disease whose intestinal form shares several characterictics with crohn's disease (cd), like the ileocolonic location predominantly and the presence of granuloma. | 1999 | 10343141 |
| anisakidae larval infection in fish fillets sold in belgium. | fish fillets (n = 1760) sold on the belgium market were examined for anisakidae larval infection. seven sea fish species were examined by means of the candling technique over a period of 4 months, from november 1996 to february 1997. third-stage larvae of anisakis simplex and pseudoterranova decipiens were identified. a. simplex was the most abundant species with a prevalence of 84.5%, and was found in 192 of the examined fillets (10.9%). pollock (pollachius pollachius) was the most heavily infe ... | 1999 | 10321017 |
| effects of anisakis simplex on nitric oxide production in j774 macrophages. | since nitric oxide (no) was recognized as a potent microbicidal agent, its role in host defence against intracellular parasites has been widely demonstrated. recent evidence suggests a role for no in combating extracellular and multicellular pathogens. this defence activity has been demonstrated toward the larvae of schistosoma mansoni, microfilariae of onchocerca linealis, several stages of brugia malayi and protoscoleces of echinococcus multilocularis. many parasites suppress th1 lymphocytes a ... | 1998 | 10225390 |
| [hypersensitivity to anisakis simplex: apropos of 36 cases]. | the authors report a group of 36 cases of hypersensitivity to anisakis simplex, a fish parasite. they examined 11 boys and 25 girls who attended the allergy service of the central hospital marques of valdecilla in santander (spain), between 12th may 1997 and 26th june 1998, for history of acute urticaria, most often after an aggravation of an allergic pathology. all were given an immunological examination that was composed of: food investigation, prick test, measurement of total and specific ige ... | 1999 | 10070595 |