Publications

the selenium requirements of the hen and effects of a deficiency.	hens were fed a diet low in selenium made up mostly of corn and torula yeast. egg production and hatchability increased significantly when selenium was supplemented, while the percentage of infertile eggs and early dead embryos decreased. anemia also resulted from the deficiency. a total of .05 mg. selenium per kg. (.04 from the basal diet plus .01 supplemental) was adequate to prevent signs of deficiency in a diet which contained no supplemental vitamin e or antioxidant. in a second experiment ...	1977	611492
dna sequence similarity, cell wall mannans and physiological characteristics in some strains of candida utilis, hansenula jadinii and hansenula petersonii.	the physiological characteristics, proton magnetic resonance spectra of cell wall mannans, dna base composition and dna sequence similarity of some strains of candida utilis, hansenula jadinii and hansenula petersonii were examined. it was found that c. utilis was not distinguishable from h. jadinii by any of these criteria. these findings show the close genetic relationship between c. utilis and its perfect form h. jadinii. in contrast, h. petersonii was found to differ from c. utilis and h. ja ...	1979	576029
determination of conspecificity of candida utilis and hansenula jadinii through dna reassociation.		2000	575194
the primary structure of cytoplasmic initiator transfer ribonucleic acid from torulopsis utilis.	cytoplasmic initiator transfer ribonucleic acid (trnainit) was purified from bulk torulopsis (candida) utilis trna by a series of column chromatography procedures. sequence analysis of the products of complete and partial digestion of this trna with ribonuclease a [ec 3.1.4.22] and ribonuclease t1 [ec 3.1.4.8] enabled us to determine the complete primary structure of the molecule. the chain length of this trna was 76, including 11 modified nucleotides. the structure of the trna was arranged into ...	1979	573264
structure and function of 5s ribosomal ribonucleic acid from torulopsis utilis. iv. detection of exposed guanine residues by chemical modification with kethoxal.	the reaction of torulopsis (candida) utilis 5s ribosomal rna with kethoxal (beta-ethoxy-alpha-ketobutyraldehyde) was studied in an attempt to identify the exposed guanine residues. at most 7-8 out of 32 guanine residues in t.utilis 5s rna were kethoxalated after reaction at 37 degrees c for 4 h in the presence of magnesium ions. localization of the kethoxalated guanine residues in t.utilis 5s rna was achieved by sequence analyses of rnase t1 digests of the kethoxalated 5s rna. these analyses sho ...	1978	568134
a general method for characterizing naturally occurring folate compounds, illustrated by characterizing torula yeast (candida utilis) folates.		1978	567943
structure and function of 5s ribosomal ribonucleic acid from torulopsis utilis. iii. detection of single-stranded regions by digestion with nuclease s1.	identification of single-stranded regions in torulopsis utilis 5s rna was attempted by the use of nuclease s1, a single-strand specific endonuclease. when t. utilis 5s rna was subjected to prolonged incubation with nuclease s1, about 50% of the substrate 5s rna remained as large oligonucleotide "cores." such nuclease s1-resistant fragments were purified and sequenced by column chromatographic procedures. these analyses revealed that regions around positions 12, 40, 57, and 110 are in exposed sin ...	1977	560370
reduction of hepatic lipid deposition in laying hens by dietary selenium-yeast interaction.	experiments were conducted to study the effect of chromiun and selenium on liver lipid deposition and incidence of liver hemorrhage in caged layers. commercial strains of layers were fed ad libitum equicaloric and isonitrogenous diets. corn-torula dried yeast diets containing added selenium (.1 microgram/g) with or without supplementary chromium (10 microgram/g) significantly reduced total liver lipid and liver hemorrhage. the effects of protein source (soybean meal vs. yeast) and selenium were ...	1979	537985
effect of graded dietary levels of selenium on tracheal carcinomas induced by 1-methyl-1-nitrosourea.	an investigation was conducted to determine the effect of the dietary level of selenium on the induction of tracheal cancer by 1-methyl-1-nitrosourea (mnu). male syrian golden hamsters received intratracheal instilations of a 0.5% mnu solution once weekly for 12 weeks. two weeks prior to the initiation of carcinogen treatment, animals were placed on a semisynthetic, 30% torula yeast diet to which either no selenium or 1 or 5 mg selenium/kg of diet as sodium selenite was added. animals were maint ...	1979	509405
glutathione peroxidase acitvity in intestinal and liver tissues of rats fed various levels of selenium, sulfur and alpha-tocopherol.	the effects of various levels of selenium, alpha-tocopherol and sulfur on glutathione peroxidase (gsh-px) activity in intestinal and liver tissues were determined in male rats fed corn-soybean or torula yeast diets. rats fed corn-soybean diets had greater gsh-px activity in the small intestine, colon and liver tissues, catalase activity and selenium in the liver, and body weight gains than those fed torula yeast diets. gsh-px activity in the small intestine, colon, and liver tissues as well as c ...	1979	430245
oxygen isotope fractionation during respiration for different temperatures of t. utilis and e. coli k12.	the temperature dependence of the oxygen isotope fractionation factor during respiration has been examined for two different microorganisms, namely torulopsis utilis and escherichia coli k12 representing a yeast and a bacterium, respectively. the investigation covered a temperature range of 18 degrees c, that is from 16 degrees c to 34 degrees c for t. utilis and from 19 degrees c to 37 degrees c for e. coli k12. within this temperature range the fractionation factor of t. utilis increases by 0. ...	1979	396549
nucleotide sequence of leucine transfer rna 1 from candida (torulopsis) utilis.	two species of 32p-labelled leucine trna were highly purified from candida (torulopsis) utilis by successive column chromatographies. the purified major species of leucine trna 1 was completely digested with ribonuclease t1 [ec 3.1.4.8] and with pancreatic ribonuclease a [ec 3.1.4.22]. the resulting fragments were fractionated, and their nucleotide sequences were determined according to barrell (1). the results of analyses of the two ribonuclease digests were consistent with each other, and indi ...	1978	350863
sequence of an oligonucleotide derived from the 3' end of each of the four brome mosaic viral rnas.	a 3'-terminal oligonucleotide fragment, 161 bases long, can be obtained from each of the four brome mosaic virus rnas by means of nuclease digestion. like the four intact brome mosaic virus rnas, each fragment accepts tyrosine in a reaction catalyzed by wheat germ aminoacyl-trna synthetase. the complete nucleotide sequence of the rna 4 fragment has been determined by use of standard radiochemical methods. comparative data for the fragments from rnas 1, 2, and 3 show that they have nearly the sam ...	1977	270723
in vitro binding of 2-acetaminofluorene to rna and protein with rat liver microsomes.	covalent binding of 2-acetaminofluorene[9-14c] with exogenous torula yeast rna and endogenous protein was investigated in liver microsome system in vitro. the binding to protein was 100 times higher than that to rna. requirement of nadph, effectiveness of methylcholanthrene treatment, and inhibition by 7,8-benzoflavone suggest possible involvement of mixed-function oxidases in this binding. the binding was not due to contaminated cytosol in the microsome fraction. addition of cytosol, sulfate io ...	1978	30668