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bromelain enzyme from pineapple: in vitro activity study under different micropropagation conditions.the aim of this work was to evaluate the activity of bromelain in pineapple plants (ananas comosus var. comosus), pérola cultivar, produced in vitro in different culture conditions. this enzyme, besides its pharmacological effects, is also employed in food industries, such as breweries and meat processing. in this work, the enzymatic activity was evaluated in the tissues of leaves and stems of plants grown in culture medium without plant growth regulator. the most significant levels of bromelain ...201222736274
isolation and characterization of a flowering locus t homolog from pineapple (ananas comosus (l.) merr).flowering locus t (ft)-like genes are crucial regulators of flowering in angiosperms. a homolog of ft, designated as acft (genbank id: hq343233), was isolated from pineapple cultivar comte de paris by reverse transcriptase polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race). the cdna sequence of acft is 915 bp in length and contains an orf of 534 bp, which encodes a protein of 177 aa. molecular weight was 19.9 kda and isoelectric point was 6.96. the deduced protein seq ...201222710136
anaerobic digestion of pineapple pulp and peel in a plug-flow reactor.the objective of this research was to study the production of biogas by using pineapple pulp and peel, the by-products from fruit processing plants, in a plug-flow reactor (17.5 l total volume). the effects of feed concentration, total solids (ts) and hydraulic retention time (hrt) on degradation of the waste were investigated. the increase of pineapple pulp and peel of 2% (wt/vol) at hrt 7 d to 4% (wt/vol) at hrt 10 d showed increases in biogas production rate, biogas yield and methane yield - ...201222705859
occupational exposure to pesticides and parkinson's disease: a systematic review and meta-analysis of cohort studies.to systematically review available cohort studies and estimate quantitatively the association between occupational exposure to pesticides and parkinson's disease (pd).201222698719
chemical shift assignments of the canecystatin-1 from saccharum officinarum.cystatins are cysteine proteases inhibitors that are widely distributed among insects, mammalians and plants. here we report the complete resonance assignment of canecystatin-1 from saccharum officinarum obtained by heteronuclear multidimensional high-resolution nuclear magnetic resonance spectroscopy. the consensus chemical shift index was calculated and showed the presence of one α-helix (residues 27-43) and three β-strands (residues 48-74, 78-89 and 94-104), a secondary structure pattern that ...201322696137
effects of wax treatment on the physiology and cellular structure of harvested pineapple during cold storage.pineapple (ananas comosus (l.) merr. cv. 'paris') is sensitive to low temperature and highly susceptible to blackheart during cold storage, which causes serious fruit decay. this work investigated the effect of wax treatment (sta-fresh 2952, 60 g/l) on blackheart of pineapple under chilling stress. wax treatment significantly reduced blackheart symptoms after 14 days of storage and markedly delayed changes in firmness and flesh color during the whole period of storage. the weight loss of wax-tre ...201222681417
stem bromelain-induced macrophage apoptosis and activation curtail mycobacterium tuberculosis persistence.mycobacterium tuberculosis, the causative agent of tuberculosis, has a remarkable ability to usurp its host's innate immune response, killing millions of infected people annually. one approach to manage infection is prevention through the use of natural agents. in this regard, stem bromelain (sbm), a pharmacologically active member of the sulfhydryl proteolytic enzyme family, obtained from ananas comosus and possessing a remarkable ability to induce the innate and acquired immune systems, is imp ...201222615313
extensive citrus triploid hybrid production by 2x×4x sexual hybridizations and parent-effect on the length of the juvenile phase.the citrus fresh market demands the production of seedless citrus fruits, as seedy fruits are not accepted by consumers. the recovery of triploid plants has proven to be the most promising approach to achieve this goal, since triploids have very low fertility, are generally seedless and do not induce seeds in other cultivars by cross pollination. triploid plants can be recovered by 2x×4x sexual hybridization. in this work, we present an effective methodology to recover triploid plants from 2x×4x ...201222614256
synergy between enge, xyna and mana from clostridium cellulovorans on corn stalk, grass and pineapple pulp substrates.the synergistic interaction between various hemi/cellulolytic enzymes has become more important in order to achieve effective and optimal degradation of complex lignocellulose substrates for biofuel production. this study investigated the synergistic effect of three enzymes endoglucanase (enge), mannanase (mana) and xylanase (xyna) on the degradation of corn stalk, grass, and pineapple fruit pulp and determined the optimal degree of synergy between combinations of these enzymes. it was establish ...201122558536
bromelain, a cysteine protease from pineapple (ananas comosus) stem, is an inhibitor of fungal plant pathogens.this study aimed to evaluate the effect of bromelain, a cysteine protease isolated from pineapple (ananas comosus), on growth of several agronomically important fungal pathogens.201222537505
production of cellulases from aspergillus niger ns-2 in solid state fermentation on agricultural and kitchen waste residues.various agricultural and kitchen waste residues were assessed for their ability to support the production of a complete cellulase system by aspergillus niger ns-2 in solid state fermentation. untreated as well as acid and base-pretreated substrates including corn cobs, carrot peelings, composite, grass, leaves, orange peelings, pineapple peelings, potato peelings, rice husk, sugarcane bagasse, saw dust, wheat bran, wheat straw, simply moistened with water, were found to be well suited for the or ...201222503148
impact of six fruits--banana, guava, mangosteen, pineapple, ripe mango and ripe papaya--on murine hepatic cytochrome p450 activities.the effects of six thai fruits, namely banana, guava, mangosteen, pineapple, ripe mango and ripe papaya, on cytochrome p450 (p450) activities were investigated. the median inhibitory concentrations (ic(50) ) of each of the fruit juices on cyp1a1, cyp1a2, cyp2e1 and cyp3a11 activities were determined. pineapple juice showed the strongest inhibitory effect against all the evaluated p450 isozyme activities in mouse hepatic microsomes, followed by mangosteen, guava, ripe mango, ripe papaya and banan ...201222499231
a highly sensitive single-tube nested pcr assay for the detection of pineapple mealybug wilt associated virus-2 (pmwav-2).an assay was developed for the detection of pineapple mealybug wilt associated virus-2 (pmwav-2), an important factor in the etiology of mealybug wilt of pineapple. the assay combines reverse transcription of rna isolated from pineapple with a specific and very sensitive, single, closed-tube nested polymerase chain reaction (pcr) to amplify a segment of the coat protein gene of the pmwav-2. the outer primers were designed to anneal at higher temperatures than the nested primers to prevent primer ...201222484612
characterization and expression analysis of acserk2, a somatic embryogenesis and stress resistance related gene in pineapple.the isolation and characterization of acserk2, another somatic embryogenesis receptor-like kinase (serk) gene homolog, confirmed that there was a serk gene family in pineapple. acserk2 shared high similarity to acserk1 and other serks, containing all the characteristic domains of the serk family. the expressed recombinant protein of pet-acserk2 was soluble. acserk2 expression was effectively induced by 2,4-dichlorophenoxyacetic acid (2,4-d) and maintained in high level at the early stage of soma ...201222465534
in vitro anthelmintic activity of crude extracts of selected medicinal plants against haemonchus contortus from sheep.ethanol extracts of 25 plant species were screened for anthelmintic effects against haemonchus contortus. ethanol extracts of each plant were used at various concentrations (10, 20 and 30%) to treat 10-day faecal cultures, incubated at 27°c with control cultures which were treated with ethanol for 48 h. five plants with high efficacies (ananas comosus, aloe ferox, allium sativum, lespedeza cuneata and warburgia salutaris) were selected from the first screening for further investigation using eth ...201322449714
bioactivity of phytochemicals in some lesser-known plants and their effects and potential applications in livestock and aquaculture production systems.livestock and aquaculture production is under political and social pressure, especially in the european union (eu), to decrease pollution and environmental damage arising due to animal agriculture. the eu has banned the use of antibiotics and other chemicals, which have been shown to be effective in promoting growth and reducing environment pollutants because of the risk caused to humans by chemical residues in food and by antibiotic resistance being passed on to human pathogens. as a result of ...200722444893
loss-of-function screen reveals novel regulators required for drosophila germline stem cell self-renewal.the germline stem cells (gscs) of drosophila melanogaster ovary provide an excellent model system to study the molecular mechanisms of stem cell self-renewal. to reveal novel factors required for drosophila female gsc maintenance and/or division, we performed a loss-of-function screen in gscs by using a collection of p-element-induced alleles of essential genes. mutations in genes of various functional groups were identified to cause defects in gsc self-renewal. here we report that a group of mu ...201222413088
a participatory assessment of ecosystem services and human wellbeing in rural costa rica using photo-voice.human well-being is intricately connected to ecosystem services. a keystone contribution to the ecosystem service literature has been the millennium ecosystem assessment, ma, (ecosystems and human well-being: a framework for assessment, island press, washington, dc; 2003, 2005). much of the work on ecosystem services to date has focused on the assessment and classification of environmental functions. the need for inclusion of community perspectives in ecosystem assessments has been widely recogn ...201222402931
bacterial reduction of cr(vi) at technical scale--the malaysian experience.the bacterial reduction of cr(vi) from industrial wastewater was evaluated using a 2.0-m(3) bioreactor. liquid pineapple waste was used as a nutrient for the biofilm community formed inside the bioreactor. the use of rubber wood sawdust as packing material was able to immobilize more than 10(6) cfu ml(-1) of acinetobacter haemolyticus cells after 3 days of contact time. complete reduction of 15-240 mg l(-1) of cr(vi) was achieved even after 3 months of bioreactor operation. cr(vi) was not detect ...201222350941
adsorption and kinetic behavior of recombinant multifunctional xylanase in hydrolysis of pineapple stem and bagasse and their hemicellulose for xylo-oligosaccharide production.a recombinant multifunctional xylanase (rmfxase) was utilized to hydrolyze xylan in pineapple stems and bagasse. the feasibility of using rmfxase and the two agricultural by-products for producing xylo-oligosaccharides was demonstrated. adsorption dynamics showed that lignin and cellulose delayed the adsorption equilibrium. the adsorption data fit the langmuir-type adsorption isotherm. the extent of hydrolysis was quantified by measuring the reducing and total sugars in substrate-rmfxase complex ...201222342034
population dynamics of earthworms in relation to soil physico-chemical parameters in agroforestry systems of mizoram, india.earthworm population dynamics was studied in two agroforestry systems in the tropical hilly terrain of mizoram, north-east india, over a period of 24 months, from july 2002 to june 2004. two sites of agroforestry situated at sakawrtuichhun (skt) and pachhunga university college (puc) campus, aizawl, having pineapple as the main crop, were selected for detail studies on population dynamics. five of the total twelve species of earthworm reported from the state were recorded in the study sites. the ...201122319875
cloning and expression analysis of a pistillata homologous gene from pineapple (ananas comosus l. merr).pistillata (pi)-like genes are crucial regulators of flowering in angiosperms. a homologue of pi, designated as acpi (genbank accession number hq717796), was isolated from pineapple cultivar comte de paris by reverse transcriptase polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race). the cdna sequence of acpi is 907 bp in length and contains an open reading frame of 594 bp, which encodes a protein of 197 amino acids. the molecular weight was 2.29 kda and the isoelectric ...201222312303
[development of cereal bar with pineapple skin].the cereal bars are multi-component products consisting of cereals, dried fruit and syrup binder and may be added to the consumable parts of fruits and vegetables which usually are not exploited and have high nutritional value, thereby reducing food waste. it was developed a jam with pineapple skin, which it was utilized in 13.5% in the cereal bar formulation. the cereal bar was sensorial evaluated and had its centesimal and mineral composition determined. the new product achieved average of 8.3 ...201122308949
production and characterization of violacein by locally isolated chromobacterium violaceum grown in agricultural wastes.the present work highlighted the production of violacein by the locally isolated chromobacterium violaceum (genbank accession no. hm132057) in various agricultural waste materials (sugarcane bagasse, solid pineapple waste, molasses, brown sugar), as an alternative to the conventional rich medium. the highest yield for pigment production (0.82 g l⁻¹) was obtained using free cells when grown in 3 g of sugarcane bagasse supplemented with 10% (v/v) of l-tryptophan. a much lower yield (0.15 g l⁻¹) wa ...201222278051
real-time magnetic resonance imaging of normal swallowing.to evaluate the use of a novel real-time magnetic resonance imaging (mri) technique for the assessment of normal swallowing dynamics.201222271426
amperometric fructose sensor based on ferrocyanide modified screen-printed carbon electrode.the first fructose sensor using a commercial screen-printed ferrocyanide/carbon electrodes (spfce) is reported here. the ferrocyanide is included in the carbon ink of the commercial screen-printed carbon electrode. the immobilization of enzyme d-fructose dehydrogenase (fdh) was carried out in an easy way. an aliquot of 10μl fdh was deposited on the electrode surface and left there until dried (approximately 1h) at room temperature. the sensor, so constructed, shows a good sensitivity to fructose ...201222265522
antioxidant, antimicrobial, 15-lox, and ages inhibitions by pineapple stem waste.pineapple stem has been extensively used for bromelain extraction; however, almost no attention has been given to the waste obtained during bromelain manufacturing. in this regard, antioxidant, antimicrobial, and inhibitions against 15-lipoxygenase and advanced glycation end product formations by pineapple stem waste (psw) obtained during bromelain manufacturing process were studied. the psw had moderate bioactivities in all the performed assays. it also showed a considerable inhibition against ...201222260109
soft fruit traceability in food matrices using real-time pcr.food product authentication provides a means of monitoring and identifying products for consumer protection and regulatory compliance. there is a scarcity of analytical methods for confirming the identity of fruit pulp in products containing soft fruit. in the present work we have developed a very sensible qualitative and quantitative method to determine the presence of berry dnas in different food matrices. to our knowledge, this is the first study that shows the applicability, to soft fruit tr ...200922253987
inhibition or enhancement by 4 pacific island food plants against cancers induced by 2 amino-3-methylimidazo[4,5-f]quinoline in male fischer 344 rats.a 1-yr carcinogenicity bioassay was conducted in rats fed 2-amino-3-methylimidazo[4,5-f]quinoline (iq), simultaneously with ain-76/ high-fat (hf) diet alone, or with 10% starch replaced with kumara, pineapple, coconut, or taro, prepared as for a human diet. all of the non-iq treated control, kumara, pineapple, or taro but not coconut-fed rats survived to 1 yr. none of the iq-fed animals survived to 1 yr and although there were minor survival time differences among the groups, none was statistica ...201222211787
high fat meal increase of il-17 is prevented by ingestion of fruit juice drink in healthy overweight subjects.an emerging role of il-17 in the inflammatory response associated with pathogenesis of neurodegeneration has been recently suggested. however, though diet represents a key factor in the modulation of inflammatory processes, evidence is not currently available on the nutritional regulation of il-17 in humans. in a double blind, randomized, placebo controlled, crossover study, we investigated the effect of high fat meal (hfm) on il-17 circulating levels in presence of a placebo (hfm-p) or with a f ...201222211683
development of ests and data mining of pineapple est-ssrs.improving the quality of the non-climacteric fruit, pineapple, is possible with information on the expression of genes that occur during the process of fruit ripening. this can be made known though the generation of partial mrna transcript sequences known as expressed sequence tags (ests). ests are useful not only for gene discovery but also function as a resource for the identification of molecular markers, such as simple sequence repeats (ssrs). this paper reports on firstly, the construction ...201222207174
[consumption of fruit juices and fruit drinks: impact on the health of children and teenagers, the dentist's point of view].the french dietary guidelines published in 2001 recommend daily consumption of 5 portions of fruit or vegetable. despite this advice, the consumption of fruit in france, especially in the north of france, is low, whereas sale of 100% fruit juices, fruit drinks, and fruit-flavored beverages is increasing. the impact of contemporary changes in beverage patterns on dental caries has received less attention than the impact on childhood obesity. nevertheless, the cariogenic potential of soft drinks i ...201222206891
bromelain-induced apoptosis in gi-101a breast cancer cells.bromelain is a proteolytic enzyme extracted from the stems and the immature fruits of pineapple that was found to be antitumorigenic in different in vitro models. bromelain has been reported to promote apoptosis, particularly in breast cancer cells, with the up-regulation of c-jun n-terminal kinase and p38 kinase. our study was designed to determine if bromelain could induce apoptosis in gi-101a breast cancer cells. gi-101a cells were treated with increasing concentrations of bromelain for 24 ho ...201222191568
effect of flammulina velutipes on spent-hen breast meat tenderization.an experiment was carried out to investigate the effects of powdered vegetable dip sauces to improve the tenderness of spent-hen breast meat. our overall purpose was to find lower-priced materials for the tenderization of spent-hen breast meat. the spent-hen breast meat was dipped into vegetable powder for 24 h at 4°c, and then the samples were analyzed. in the results for vegetable-powder treated samples, those treated with papain and pineapple had higher (p ≤ 0.05) myofibrillar fragmentation i ...201222184449
the physiology of ex vitro pineapple (ananas comosus l. merr. var md-2) as cam or c3 is regulated by the environmental conditions.many plant species grown under in vitro controlled conditions can be used as models for the study of physiological processes. adult pineapple can display cam physiology while in vitro it functions as a c3 plant. ex vitro ananas comosus has plastic morphology and physiology, both easy to modify from c3 to cam by changing the environmental conditions. the yield of survival for a rentable propagation protocol of pineapple is closely related with the c3/cam shift and the associated physiological cha ...201222134875
effect of acidic agents on surface roughness of dental ceramics.an increase in surface roughness of ceramics may decrease strength and affect the clinical success of ceramic restorations. however, little is known about the effect of acidic agents on ceramic restorations. the aim of this study was to evaluate the surface roughness of dental ceramics after being immersed in acidic agents.201122132009
Candida krusei and Kloeckera apis inhibit the causal agent of pineapple fusariosis, Fusarium guttiforme.Studies based on microbial ecology and antagonistic interactions play an important role in the development of new alternative strategies in controlling plant pathogens and are relevant to further biotechnological applications. Antagonistic interactions between the yeasts Candida krusei and Kloeckera apis isolated from rotten pineapple fruits, and two isolates of the pathogenic filamentous fungus Fusarium guttiforme (Syn.: Fusarium subglutinans f. sp. ananas) resistant and susceptible to fungicid ...201122115444
Biotransformation of pineapple juice sugars into dietetic derivatives by using a cell free oxidoreductase from Zymomonas mobilis together with commercial invertase.An easy procedure for cell free biotransformation of pineapple juice sugars into dietetic derivatives was accomplished using a commercial invertase and an oxidoreductase from Zymomonas mobilis. First, pineapple juice sucrose was quantitatively converted into glucose and fructose by invertase, thus increasing the concentration of each monosaccharide in the original juice to almost twice. In a second step, glucose-fructose oxidoreductase (GFOR) transformed glucose into gluconolactone, and fructose ...201122112775
quantification of allyl hexanoate in pineapple beverages and yogurts as a case study to characterise a source of uncertainty in dietary exposure assessment to flavouring substances.one source of uncertainty in the estimation of dietary exposure to flavouring substances is the uncertainty in the occurrence and concentration levels of these substances naturally present or added to foodstuffs. the aim of this study was to assess the variability of concentration levels of allyl hexanoate, considered as a case study, in two main food categories to which it is often added: pineapple juice-based beverages and yogurts containing pineapple. thirty-four beverages and 29 yogurts, wit ...201222043838
a comparative study of ph modulation and trace elements of various fruit juices on enamel erosion: an in vitro study.fruit juices are popular worldwide with children of all ages as they are sweet and perceived to be healthful. this in vitro study was sought to measure ph of 10 different fruit juices, to find out possible erosive effects on human dental enamel of 40 extracted sound premolars and also to measure fluoride and trace elements of these juices. the estimation of ph of fruit juices was done by using systronic uph 362 ph meter. the erosive effects of fruit juices were tested by using polarized light mi ...201121985876
glycaemic response to some commonly eaten fruits in type 2 diabetes mellitus.it is not known which of the commonly consumed fruits in nigeria are suitable for persons with diabetes mellitus especially with regards to the attendant plasma glucose response (pgr) to consumption of such fruits.201121984455
use of a multivariate approach to assess the incidence of alicyclobacillus spp. in concentrate fruit juices marketed in argentina: results of a 14-year survey.the purpose of this study was to determine the incidence of alicyclobacillus spp. in fruit/vegetable juices (concentrated pulps and clarified and non-clarified juices) marketed in argentina between 1996 and 2009. the presence of alicyclobacillus was determined in a total of 8556 samples of fruit and vegetable juices (apple, pear, grape, peach, blend of juices, tangerine, pineapple, orange, mango, plum, guava, apricot, lemon, banana, kiwi, carrot, strawberry, grapefruit, and beetroot) collected i ...201121962940
comparison of the carbon-sequestering abilities of pineapple leaf residue chars produced by controlled combustion and by field burning.this study was undertaken to compare the chemical properties and yields of pineapple leaf residue (plr) char produced by field burning (cf) with that produced by a partial combustion of air-dried plr at 340 °c for 3 h in a furnace (cl). higher total c, lignin content, and yield from cl as well as the presence of aromatic compounds in the fourier transform infrared spectra of the char produced from cl suggest that the cl process was better in sequestering c than was the cf process. although the c ...201121958525
juice blends--a way of utilization of under-utilized fruits, vegetables, and spices: a review.the post-harvest shelf life of maximum of fruits and vegetables is very limited due to their perishable nature. in india more then 20-25 percent of fruits and vegetables are spoiled before utilization. despite being the world's second largest producer of fruits and vegetables, in india only 1.5 percent of the total fruits and vegetables produced are processed. maximum amounts of fruit and vegetable juices turn bitter after extraction due to conversion of chemical compounds. in spite of being und ...201121929332
content of phenolic compounds in aerial parts of chamomilla suaveolens from estonia.the content of total polyphenols, flavonoids and coumarins in the aerial parts of pineapple weed (chamomilla suaveolens (pursh) rydb.), growing wild in estonia, was determined chromato-spectrophotometrically, and individual polyphenols were quantified using hplc-dad-ms/ms. the total content of polyphenols was rather similar in flowers (9.1-11.5%) and in whole aerial parts (8.4-10.9%) of pineapple weed. the upper one-third (1/3) and upper two-thirds (2/3) of the aerial parts contained more flavon ...201121922910
the role of aqueous extract of pineapple fruit parts on the healing of acute crush tendon injury.the pineapple plant contains the enzyme bromelain which has been acclaimed to reduce pain and swellings following acute muscle injuries as well as carotenoids and polyphenols which are powerful antioxidants. it is yet to be determined if these constituents are distributed throughout the plant and what effect they have on the healing of acute tendon injuries.201021913532
gradual surface degradation of restorative materials by acidic agents.the aim of this study was to investigate the effect of acidic agents on surface roughness and characteristics of four restorative materials. fifty-two discs were created from each restorative material: metal-reinforced glass ionomer cement (ketac-s), resin-modified glass ionomer cement (fuji ii lc), resin composite (filtek z250), and amalgam (valiant-phd); each disc was 12 mm in diameter and 2.5 mm thick. the specimens were divided into four subgroups (n=13) and immersed for 168 hours in four st ...201321903509
genomic analysis of grapevine leafroll associated virus-5 and related viruses.the grapevine leafroll-associated viruses (glravs) (closteroviridae) represent an emerging threat to world grape production. one group of glravs within the genus ampelovirus, the glrav-4-like viruses (glrav-4lvs), contains a fragmented collection of seven viruses only two of which (glrav-pr and glracv) are fully sequenced. here in reporting the sequence of glrav-5, a member of glrav-4lvs, we identify genomic elements common to the glrav-4lv group. exclusive properties include a highly conserved ...201121893115
evaluation of antioxidant activity and antiproliferative effect of fruit juices enriched with pycnogenol® in colon carcinoma cells. the effect of in vitro gastrointestinal digestion.the aim of this study was to examine the effect of in vitro gastrointestinal digestion on the antioxidant and antiproliferative effect of fruit juices enriched with pycnogenol® (0.5 g/l) on a colon carcinoma cell line (caco-2). the total phenolic concentration (tpc), antioxidant activity and inhibition cell growth were studied in fresh and digested pineapple juice and red fruits juice (both enriched with pine bark extract and not). after in vitro digestion the level of detectable phenolic compou ...201121887808
modification of pineapple peel fibre with succinic anhydride for cu2+, cd2+ and pb2+ removal from aqueous solutions.research on chemical modification of pineapple peel fibre with succinic anhydride was carried out to create a novel adsorbent for cu2+, cd2+ and pb2+ removal from aqueous solution. after pretreatment with iso-propyl alcohol and naoh, pineapple peel fibre was modified via reaction with succinic anhydride for introduction of carboxylic functional groups. the modified pineapple peel fibre was characterized with fourier transform infrared (ftir) spectroscopy and evaluated for its adsorptive ability ...201321879549
headspace-solid phase microextraction coupled to gas chromatography-combustion-isotope ratio mass spectrometer and to enantioselective gas chromatography for strawberry flavoured food quality control.authenticity assessment of flavoured strawberry foods was performed using headspace-solid phase microextraction (hs-spme) coupled to gas chromatography-combustion-isotope ratio mass spectrometry (gc-c-irms). an authenticity range was achieved, investigating on the carbon isotope ratio of numerous selected aroma active volatile components (methyl butanoate, ethyl butanoate, hex-(2e)-enal, methyl hexanoate, buthyl butanoate, ethyl hexanoate, hexyl acetate, linalool, hexyl butanoate, octyl isovaler ...201121872872
dissolution of undigested food in a diabetic patient's stomach by drinking pineapple juice. 201121837823
ananas comosus: is the unripe fruit juice an abortifacient in pregnant wistar rats?abstract objective the juice of unripe pineapple (ananas comosus) was investigated for abortifacient activity in pregnant wistar rats. method animals in groups a, b, c and d received orally 0.5 ml of distilled water, 250, 500 and 1000 mg/kg body weight of the juice, respectively, once daily from day 7 until day 14 of pregnancy. results the juice contained tannins, cardenolides, dienolides, cardiac glycoside and flavonoids. the number and weights of live fetuses, number of implantation sites, cor ...201121827351
extraction of pesticides, dioxin-like pcbs and pahs in water based commodities using liquid-liquid microextraction and analysis by gas chromatography-mass spectrometry.water based samples such as flavored drinks, juices and drinking water may contain contaminants at ultra trace level belonging to different chemical classes. a novel, simple, low-cost and fast method was developed and validated for trace residue extraction of pesticides, dioxin-like pcbs and pahs from water and water based samples followed by analysis through gas chromatography (gc) coupled with time-of-flight mass spectrometry (tofms). the extraction solvent type, volume; sample volume and othe ...201121820665
resonance assignments and secondary structure of a phytocystatin from ananas comosus.a cdna encoding a cysteine protease inhibitor, cystatin was cloned from pineapple (ananas comosus l.) stem. this clone was constructed into an expression vector and expressed in e. coli and purified to homogeneous. the recombinant pineapple cystatins (accys) showed effectively inhibitory activity toward cysteine proteases including papain, bromelain, and cathepsin b. in order to unravel its inhibitory action from structural point of view, multidimensional heteronuclear nmr techniques were used t ...201221814766
extraction of bromelain from pineapple peels.large amount of pineapple peels (by-products) is left over after processing and they are a potential source for bromelain extraction. distilled water (di), di containing cysteine and ethylenediaminetetraacetic acid (edta) (di-ce), sodium phosphate buffer ph 7.0 (pb) and pb containing cysteine and edta (pb-ce) were used as extractants for bromelain from the pineapple peels. the highest bromelain activity was obtained when it was extracted with pb-ce (867 and 1032 units for nang lae and phu lae cu ...201121813595
genetic and phenotypic variation of fusarium proliferatum isolates from different host species.fusarium proliferatum (matsushima) nirenberg is a common pathogen infecting numerous crop plants and occurring in various climatic zones. it produces large amounts of fumonisins, a group of polyketide-derived mycotoxins. fumonisin biosynthesis is determined by the presence and activity of the fum cluster, several co-regulated genes with a common expression pattern. in the present work, we analyzed 38 f. proliferatum isolates from different host plant species, demonstrating host-specific polymorp ...201121796391
emerging risk of infestation and contamination of dried fruits by mites in the czech republic.the introduction of live insects into human food is rare in developed countries. however, we report, for the first time, an emerging risk that exists from dried fruit in central europe. recently, massive and frequent infestation of dried fruit imported from the mediterranean region by the mite, carpoglpyhus lactis l. (acarina: carpoglyphidae), has been found. in 180 samples taken from supermarkets, 13% were contaminated; the contamination levels ranged from 0 to 660 mites per g of dried fruit. t ...201121793685
effect of pineapple peel extract on total phospholipids and lipid peroxidation in brain tissues of rats.to investigate the ability of the methanolic extract of pineapple peel to attenuate alcohol-induced changes in total phospholipids and lipid peroxidation in brain tissues.201121771449
colonization of citrus seed coats by 'candidatus liberibacter asiaticus': implications for seed transmission of the bacterium.huanglongbing is an economically damaging disease of citrus associated with infection by 'ca. liberibacter asiaticus'. transmission of the organism via infection of seeds has not been demonstrated but is a concern since some citrus varieties, particularly those used as rootstocks in commercial plantings are propagated from seed. we compared the incidence of detection of 'ca. liberibacter asiaticus' dna in individual fruit peduncles, seed coats, seeds and in germinated seedlings from 'sanguenelli ...201121714779
quantitative analysis of essential oils in perfume using multivariate curve resolution combined with comprehensive two-dimensional gas chromatography.the use of multivariate curve resolution (mcr) to build multivariate quantitative models using data obtained from comprehensive two-dimensional gas chromatography with flame ionization detection (gc×gc-fid) is presented and evaluated. the mcr algorithm presents some important features, such as second order advantage and the recovery of the instrumental response for each pure component after optimization by an alternating least squares (als) procedure. a model to quantify the essential oil of ros ...201121704766
characteristic aroma compounds from different pineapple parts.characteristic aroma volatile compounds from different parts of cayenne pineapple were analyzed by headspace-solid phase microextraction (hs-spme) and gas chromatography-mass spectrometry (gc/ms). the main volatile compounds were esters, terpenes, ketones and aldehydes. the number and content of aroma compounds detected in pulp were higher than those found in core. in pulp, the characteristic aroma compounds were ethyl 2-methylbutanoate, ethyl hexanoate, 2,5-dimethyl-4-hydroxy-3(2h)-furanone (dm ...201121694674
inhibitory effects of bromelain, a cysteine protease derived from pineapple stem (ananas comosus), on intestinal motility in mice.bromelain (br) is a cysteine protease with inhibitory effects on intestinal secretion and inflammation. however, its effects on intestinal motility are largely unexplored. thus, we investigated the effect of this plant-derived compound on intestinal contractility and transit in mice.201121689210
identification of volatiles from pineapple (ananas comosus l.) pulp by comprehensive two-dimensional gas chromatography and gas chromatography/mass spectrometry.combining qualitative data from the chromatographic structure of 2-d gas chromatography with flame ionization detection (gc×gc-fid) and that from gas chromatography-mass spectrometry (gc/ms) should result in a more accurate assignment of the peak identities than the simple analysis by gc/ms, where coelution of analytes is unavoidable in highly complex samples (rendering spectra unsuitable for qualitative purposes) or for compounds in very low concentrations. using data from gc×gc-fid combined wi ...201121644251
use of pineapple juice with gadopentetate dimeglumine as a negative oral contrast for magnetic resonance cholangiopancreatography: a multicentric study.we evaluated the efficacy of pineapple juice with gadopentetate dimeglumine as a negative oral contrast agent for magnetic resonance cholangiopancreatography (mrcp). images were obtained before and after the intake of a negative oral contrast agent. images obtained from six different areas of the biliary tree were analyzed by three different radiologists, who were blind to the exams; scores regarding image quality were given to each area. the statistical analysis showed a significant difference ...201221630051
in silico mining for simple sequence repeat loci in a pineapple expressed sequence tag database and cross-species amplification of est-ssr markers across bromeliaceae.a collection of 5,659 expressed sequence tags (ests) from pineapple [ananas comosus (l.) merr.] was screened for simple sequence repeats (est-ssrs) with motif lengths between 1 and 6 bp. lower thresholds of 15, 7 and 5 repeat units were used to define microsatellites of the mono-, di-, and tri- to hexanucleotide repeat type, respectively. based on these criteria, 696 ssrs were identified among 3,389 est unigenes, together representing 2,840 kb. this corresponds to an average density of one ssr e ...201121625993
enhanced iron and zinc accumulation in genetically engineered pineapple plants using soybean ferritin gene.pineapple (ananas comosus l. merr., cv. "queen") leaf bases were transformed with agrobacterium tumefaciens strain eha 105 harboring the psf and pefesf plasmids with soybean ferritin cdna. four to eight percent of the co-cultivated leaf bases produced multiple shoots 6 weeks after transfer to murashige and skoog's medium supplemented with α-naphthalene acetic acid 1.8 mg/l, indole-3-butyric acid 2.0 mg/l, kinetin 2.0 mg/l, cefotaxime 400 mg/l, and kanamycin 50 mg/l. putatively transformed shoots ...201121625916
bromelain proteinase-f9 augments human lymphocyte-mediated growth-inhibition of various tumor-cells in-vitro.bromelain, a crude extract from pineapple stem containing various thiol proteases, has previously been suggested for adjuvant therapy of malignant diseases. we hence tested in vitro whether a highly purified bromelain proteinase (f9) would affect the antitumor activity by human peripheral blood lymphocytes (pbl) against mcf-7 breast cancer, kb squamous carcinoma and sk-mel-28 melanoma cells. the antiproliferative effects by pretreated pbl were determined using the microculture tetrazolium (mtt) ...199421559575
flexural strength of fluorapatite-leucite and fluorapatite porcelains exposed to erosive agents in cyclic immersion.the aim of this study was to evaluate the flexural strength of two porcelain materials (ips d.sign and ips e.max ceram) exposed to erosive agents.201121552708
effect of cassava starch coating on quality and shelf life of fresh-cut pineapple (ananas comosus l. merril cv "pérola").this research studied the influence of treatment with ascorbic acid, citric acid, and calcium lactate dipping and cassava starch edible coatings on quality parameters and shelf life of fresh-cut pineapple in slices during 12 d at 5 °c. after previous tests, the treatments selected for this study were samples dipped into antibrowning solution with 0.5% of ascorbic acid and 1% of citric acid, with and without 2% of calcium lactate and coated with 2% of cassava starch suspensions. changes in weight ...201121535677
aroma profile and volatiles odor activity along gold cultivar pineapple flesh.physicochemical attributes, aroma profile, and odor contribution of pineapple flesh were studied for the top, middle, and bottom cross-sections cut along the central axis of gold cultivar pineapple. relationships between volatile and nonvolatile compounds were also studied. aroma profile constituents were determined by headspace solid-phase microextraction at 30 °c, followed by gas chromatography/mass spectrometry analysis. a total of 20 volatile compounds were identified and quantified. among t ...201621535624
synergistic effects of sour taste and low temperature in suppressing the bitterness of aminoleban(®) en.aminoleban(®) en, a nutritional product for patients with liver failure, contains three branched-chain amino acids (bcaas): l-leucine, l-isoleucine, and l-valine. as bcaas are extremely bitter, aminoleban(®) en has a low palatability, which is a major cause of patient noncompliance. nutrients for liver failure often need to be taken for long periods, and poor medication compliance can cause serious problems, such as encephalopathy. therefore it is important to suppress the bitter taste of aminol ...201121532188
development and evaluation of a new method for sampling and monitoring the symphylid population in pineapple.background: symphylids (hanseniella sp.) are polyphagous soilborne parasites. today, symphylid populations on pineapple are monitored by observing root symptoms and the presence of symphylids at the bottom of basal leaves. the authors developed a reliable method with a bait and trap device to monitor symphylid populations in pineapple or fallow crops. the spatial distribution of the symphylid populations was evaluated using the variance/mean ratios and spatial analyses based on moran's and geary ...201121520484
postprandial glucose response to selected tropical fruits in normal glucose-tolerant nigerians.the glycemic response to commonly eaten fruits in nigeria has not been reported. therefore, this study assessed the plasma glucose response to selected fruits in nigeria.201421493998
developmental toxicity of orally administered pineapple leaf extract in rats.the extract of pineapple leaves (epl) has anti-diabetic and anti-dyslipidemic effects and can be developed into a promising natural medicine. this study was conducted to evaluate epl's effects on developmental parameters in order to provide evidence of its safety before potential medical use. five groups were included: a negative control that was given distilled water daily, a positive control that was dosed 7 mg/kg cyclophosphamide (cp) every two days, and three groups that were respectively do ...201121457745
bromelain treatment leads to maturation of monocyte-derived dendritic cells but cannot replace pge2 in a cocktail of il-1ß, il-6, tnf-a and pge2.immunotherapy using dendritic cells (dc) has shown promising results. however, the use of an appropriate dc population is critical for the outcome of this treatment, and the search for an optimal dc subset is still ongoing. the dc used in immunotherapy today are usually matured with a cytokine cocktail consisting of tnf-a, il-1ß, il-6 and pge(2). these cells have deficits in their cytokine production, particularly il-12p70, mainly because of the presence of pge(2). bromelain is a pineapple stem ...201121449940
in vivo genotoxicity evaluation of atrazine and atrazine-based herbicide on fish carassius auratus using the micronucleus test and the comet assay.atrazine is a selective triazine herbicide used to control broadleaf and grassy weeds mainly in corn, sorghum, sugarcane, pineapple, and other crops, and in conifer reforestation planting fields. it has been showed that atrazine is one of the most frequently detected pesticides in agricultural streams and rivers, over the past two decades. although the toxic properties of atrazine are well known, the data on the genotoxic effects of atrazine on aquatic organisms are rather scarce. thus, in the p ...201121443921
bromelain inhibits nuclear factor kappa-b translocation, driving human epidermoid carcinoma a431 and melanoma a375 cells through g(2) /m arrest to apoptosis.bromelain, obtained from pineapple, is already in use clinically as adjunct in chemotherapy. our objective was to test its ability to act as a sole anti-cancer agent. therefore, we describe its anti-proliferative, anti-inflammatory and subsequent anti-cancer effects in vitro, against human epidermoid carcinoma-a431 and melanoma-a375 cells. bromelain exhibited reduction in proliferation of both these cell-lines and suppressed their potential for anchorage-independent growth. further, suppression ...201121432909
effective treatment with pineapple juice in small bowel obstruction due to phytobezoar in a gastrectomized patient. 201121429030
survival of lactobacillus plantarum in model solutions and fruit juices.the aim of the work was to study the survival of lactobacillus plantarum ncimb 8826 in model solutions and develop a mathematical model describing its dependence on ph, citric acid and ascorbic acid. a central composite design (ccd) was developed studying each of the three factors at five levels within the following ranges, i.e., ph (3.0-4.2), citric acid (6-40 g/l), and ascorbic acid (100-1000 mg/l). in total, 17 experimental runs were carried out. the initial cell concentration in the model so ...201121411170
two phosphoenolpyruvate carboxykinases coexist in the crassulacean acid metabolism plant ananas comosus. isolation and characterization of the smaller 65 kda form.two phosphoenolpyruvate carboxykinase (pepck, ec 4.1.1.49) isoforms of 74 and 65 kda were found to coexist in vivo in pineapple leaves, a constitutive crassulacean acid metabolism plant. the 65 kda form was not the result of proteolytic cleavage of the larger form since extraction methods reported to prevent pepck proteolysis in other plant tissues failed to yield a single immunoreactive pepck polypeptide in leaf extracts. in this work, the smaller form of 65 kda was purified to homogeneity and ...201121398135
spider-fed bromeliads: seasonal and interspecific variation in plant performance.several animals that live on bromeliads can contribute to plant nutrition through nitrogen provisioning (digestive mutualism). the bromeliad-living spider psecas chapoda (salticidae) inhabits and breeds on bromelia balansae in regions of south america, but in specific regions can also appear on ananas comosus (pineapple) plantations and aechmea distichantha.201121385776
[analytical method of hydramethylnon in agricultural products by lc-ms/ms].a simple determination method of hydramethylnon in agricultural products by liquid chromatography-tandem mass spectrometry (lc-ms/ms) was developed. the sample was homogenized with phosphoric acid and extracted with acetone. an aliquot of crude extract was re-extracted with hexane and sat. nacl solution. in the case of tea leaf, solidification processing using ammonium chloride and phosphoric acid was performed before re-extraction with hexane. clean-up was performed using a silica-gel mini colu ...201121383534
effect of wine inhibitors on free pineapple stem bromelain activity in a model wine system.the influence of potential inhibitors, naturally present in wine, on the activity of stem bromelain was investigated in order to evaluate the applicability of this enzyme for protein stabilization in white wine. bromelain proteolytic activity was tested against a synthetic substrate (bz-phe-val-arg-pna) in a model wine system after adding ethanol, sulfur dioxide (so(2)), skin, seed, and gallic and ellagic tannins at the average range of their concentration in wine. all the inhibitors of stem bro ...201121381670
influence of unclogging agents on the surface state of enteral feeding tubes.despite standardized prevention procedures, recalcitrant clogging of enteral feeding tubes is observed, which requires recourse to varied unclogging agents. some of these agents have proved effective in routine use, but their impact on the surface state of the tube materials has never been studied. in this work, the authors tested the impact of different unclogging agents on the materials used for these tubes (polyurethane and silicone).201121378255
prevalence, concentration, spoilage, and mitigation of alicyclobacillus spp. in tropical and subtropical fruit juice concentrates.the presence of alicyclobacillus in fruit juices and concentrates poses a serious problem for the juice industry. this study was undertaken to determine the (i) prevalence, concentration, and species of alicyclobacillus in tropical and subtropical concentrates; (ii) efficacy of aqueous chlorine dioxide in reducing alicyclobacillus spp. spores on tropical and subtropical fruit surfaces; and (iii) fate of and off-flavor production by alicyclobacillus acidoterrestris in mango and pineapple juices. ...201021356453
biosorption of basic green 4 from aqueous solution by ananas comosus (pineapple) leaf powder.biosorption characteristics of ananas comosus (pineapple) leaf powder was investigated for decolorization of basic green 4 (bg 4), a cationic dye from its aqueous solutions employing a batch experimental set-up. parameters that influence the sorption process such as ph, biosorbent dosage, contact time, initial dye concentration and temperature were systematically studied. the optimum conditions for removal of bg 4 were found to be ph 9.0, contact time=150 min, biosorbent dosage=5.0 g l(-1), init ...201121349693
preparation of antioxidant enzymatic hydrolysates from honeybee-collected pollen using plant enzymes.enzymatic hydrolysates of honeybee-collected pollen were prepared using food-grade proteinase and aminopeptidases entirely of plant origin. bromelain from pineapple stem was applied (8 mau/g substrate) in the first hydrolysis stage. aminopeptidase (0.05 u/g substrate) and proline iminopeptidase (0.03 u/g substrate) from cabbage leaves (brassica oleracea var. capitata), and aminopeptidase (0.2 u/g substrate) from chick-pea cotyledons (cicer arietinum l.) were involved in the additional hydrolysis ...201121318132
effect of naturally acidic agents on microhardness and surface micromorphology of restorative materials.this study investigated the titratable acidity and erosive potential of acidic agents on the microhardness and surface micromorphology of four restorative materials.201121311608
use of dispersive liquid-liquid microextraction for the determination of carbamates in juice samples by sweeping-micellar electrokinetic chromatography.dispersive liquid-liquid microextraction (dllme) has been proposed for the extraction and preconcentration of 12 carbamate pesticides in juice samples, followed by their determination by micellar electrokinetic chromatography with diode-array detection. to improve sensitivity, an on-capillary sample concentration method based on sweeping has been developed. also, separations were performed in an extended light path fused-silica capillary; the separation buffer consisted of 100 mm borate and 50 m ...201121279629
surface characteristic changes of dental ceramics after cyclic immersion in acidic agents and titratable acidity.the potential erosive effect of acidic food, sour fruits and drinks on all-ceramic restorations used in dentistry has not been clearly documented. surface characteristic changes have been evaluated and compared for disc-shaped specimens (diameter 12.0 mm and thickness 2.0 mm) of fluorapatite-leucite and fluorapatite ceramics using various storage agents (deionized water, citrate buffer solution, pineapple juice, green mango juice, cola soft drink and 4% acetic acid). immersion in pineapple juice ...201021265437
a comparative study of the effects of bromelain and fresh pineapple juice on the early phase of healing in acute crush achilles tendon injury.bromelain, an enzyme extracted from the stem of the pineapple plant, has been reported to reduce pain and swelling in acute soft tissue injuries, but no study has been done to compare its effect with that of fresh pineapple juice on the healing of acute tendon injuries. this study compared the effects of commercial bromelain and fresh pineapple juice on tenocyte proliferation and the malondialdehyde (mda) level in the early stage of healing in a crush injury to the achilles tendon of sprague-daw ...201121254908
pesticide residue analysis of fruit juices by lc-ms/ms direct injection. one year pilot survey.for this work, thirteen types of fruit juices (orange, pineapple, peach, apple, multifruit, mango, strawberry, tomato, pear, mandarin, grape, banana and grapefruit) were selected to develop an analytical method for the analysis of 53 pesticides by direct injection in lc-ms/ms. the preparation of the samples was very simple: an aliquot of the juice was centrifuged and it was ten-times diluted prior to analysis, which allowed reducing considerably the time and cost of the analyses. besides, diluti ...201121238751
online intelligent controllers for an enzyme recovery plant: design methodology and performance.this paper focuses on the development of intelligent controllers for use in a process of enzyme recovery from pineapple rind. the proteolytic enzyme bromelain (ec 3.4.22.4) is precipitated with alcohol at low temperature in a fed-batch jacketed tank. temperature control is crucial to avoid irreversible protein denaturation. fuzzy or neural controllers offer a way of implementing solutions that cover dynamic and nonlinear processes. the design methodology and a comparative study on the performanc ...201021234106
polyphenol oxidase and peroxidase expression in four pineapple varieties (ananas comosus l.) after a chilling injury.pineapple internal browning (ib) is a chilling injury that produces enzymatic browning associated with flesh translucency. pineapple biodiversity allowed the investigation of how polyphenol oxidase (ppo) and peroxidase (pod) activities with their different isoforms are involved in the ib mechanism. fruits of four varieties that expressed ib symptoms differently, smooth cayenne (scay) and the hybrids md2, flhoran 41 (flh 41), and flhoran 53 (flh 53), were stressed by cold. the susceptible varieti ...201121133422
food allergy in ghanaian schoolchildren: data on sensitization and reported food allergy.epidemiological data on food allergy are scarce in african countries. we studied the prevalence of food sensitization in ghanaian schoolchildren.201121109750
development of a yeast strain for xylitol production without hydrolysate detoxification as part of the integration of co-product generation within the lignocellulosic ethanol process.the present study verified an applicable technology of xylitol bioconversion as part of the integration of co-product generation within second-generation bioethanol processes. a newly isolated yeast strain, candida tropicalis jh030, was shown to have a capacity for xylitol production from hemicellulosic hydrolysate without detoxification. the yeast gives a promising xylitol yield of 0.71 g(p) g(s)(-1) from non-detoxified rice straw hydrolysate that had been prepared by the dilute acid pretreatme ...201021095119
simultaneous analysis of amino acids and carboxylic acids by capillary electrophoresis-mass spectrometry using an acidic electrolyte and uncoated fused-silica capillary.a simple, low-cost capillary electrophoresis-mass spectrometry (ce-ms) method is demonstrated for the simultaneous analysis of amino acids and small carboxylic acids (glycerate, lactate, fumarate, succinate, malate, tartrate, citrate, iso-citrate, cis-aconitate, and shikimate). all ce-ms experiments were performed using a single uncoated fused-silica capillary and with a single separation electrolyte, formic acid. for ce polarity, the ce inlet was set as the anode, and the ms side was set as the ...201021080676
specificity of monoclonal antibodies to strains of dickeya sp. that cause bacterial heart rot of pineapple.during a severe outbreak of bacterial heart rot that occurred in pineapple plantations on oahu, hawaii, in 2003 and years following, 43 bacterial strains were isolated from diseased plants or irrigation water and identified as erwinia chrysanthemi (now dickeya sp.) by phenotypic, molecular, and pathogenicity assays. rep-pcr fingerprint patterns grouped strains from pineapple plants and irrigation water into five genotypes (a-e) that differed from representatives of other dickeya species, pectoba ...201021050038
improved characterization of the botanical origin of sugar by carbon-13 snif-nmr applied to ethanol.until now, no analytical method, not even isotopic ones, had been able to differentiate between sugars coming from c4-metabolism plants (cane, maize, etc.) and some crassulacean acid metabolism plants (e.g., pineapple, agave) because in both cases the isotope distributions of the overall carbon-13/carbon-12 and site-specific deuterium/hydrogen isotope ratios are very similar. following recent advances in the field of quantitative isotopic carbon-13 nmr measurements, a procedure for the analysis ...201021028824
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