Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| The influence of the fungal pathogen Mycocentrospora acerina on the proteome and polyacetylenes and 6-methoxymellein in organic and conventionally cultivated carrots (Daucus carota) during post harvest storage. | Many carrots are discarded during post harvest cold storage due to development of fungal infections, caused by, e.g., Mycocentrospora acerina (liquorice rot). We compared the susceptibility of carrots grown under conventional and organic agricultural practices. In one year, organically cultivated carrots showed 3× to 7× more symptoms than conventionally cultivated, when studying naturally occurring disease at 4 and 6months, respectively. On the other hand, we have developed a bioassay for infect ... | 2012 | 22075166 |
| [cloning and bioinformatic analysis of pqerf1 gene in panax quinquefolius]. | erf family transcription factor (tf) represented ethylene-responsive protein which harbored a conserved ap2 domain. after searching the plant transcription factor database, a total of 75 unigenes was found which contained ap2 domain from the transcriptome dataset of panax quinquefolius l. one unique sequence of erf transcript, named as pqerf1, was cloned with entire open reading frame of 933 base pairs (bp). protein prediction result indicated that the gene was localized in nucleus and had a con ... | 2011 | 22007529 |
| Uranium in vegetable foodstuffs: should residents near the Cunha Baixa uranium mine site (Central Northern Portugal) be concerned? | Large uranium accumulations in vegetable foodstuffs may present risks of human health if they are consumed. The objective of this study was to evaluate the uranium concentrations in different vegetable foodstuffs and grown in agricultural soils, which are then consumed by the residents of the village of Cunha Baixa (Portugal),-located in an former uranium mining area. This study was conducted to address concerns expressed by the local farmers as well as to provide data for uranium-related health ... | 2011 | 21964853 |
| carryover of perfluorooctanoic acid (pfoa) and perfluorooctane sulfonate (pfos) from soil to plant and distribution to the different plant compartments studied in cultures of carrots (daucus carota ssp. sativus), potatoes (solanum tuberosum), and cucumbers (cucumis sativus). | a vegetation study was carried out to investigate the carryover of perfluorooctanoic acid (pfoa) and perfluorooctane sulfonate (pfos) from soil mixed with contaminated sewage sludge to potato, carrot, and cucumber plants. analysis was done by liquid-extraction using acetonitrile with dispersive spe cleanup and subsequent hplc-ms/ms. in order to assess the transfer potential from soil, transfer factors (tf) were calculated for the different plant compartments: tf = [pfc](plant (wet substance))/[p ... | 2011 | 21905714 |
| A novel chimeric MOMP antigen expressed in Escherichia coli, Arabidopsis thaliana, and Daucus carota as a potential Chlamydia trachomatis vaccine candidate. | The major outer membrane protein (MOMP) of Chlamydia trachomatis is a highly antigenic and hydrophobic transmembrane protein. Our attempts to express the full-length protein in a soluble form in Escherichia coli and in transgenic plants failed. A chimeric gene construct of C. trachomatis serovar E MOMP was designed in order to increase solubility of the MOMP protein but with retained antigenicity. The designed construct was successfully expressed in E. coli, in Arabidopsis thaliana, and in Daucu ... | 2011 | 21903168 |
| bioactive chemicals from carrot (daucus carota) juice extracts for the treatment of leukemia. | overwhelming evidence indicates that consumption of fruits and vegetables with antioxidant properties correlates with reduced risk for cancers, including leukemia. carrots contain beneficial agents, such as β-carotene and polyacetylenes, which could be effective in the treatment of leukemia. this study investigated the effect of carrot juice extracts on myeloid and lymphoid leukemia cell lines together with normal hematopoietic stem cells. leukemia cell lines and nontumor control cells were trea ... | 2011 | 21864090 |
| spectroscopic studies on bioactive polyacetylenes and other plant components in wild carrot root. | polyacetylenes and other common plant components, such as starch, pectin, cellulose, and lignin, were studied in roots of the wild carrot (daucus carota) subspecies d. carota subsp. gummifer and d. carota subsp. maximus by raman spectroscopy. the components were measured in situ, directly in the plant tissue and without any preliminary sample preparation. the analysis was performed on the basis of the intense and characteristic key bands observed in the raman spectrum. the two main carrot polyac ... | 2011 | 21800857 |
| purification, crystallization and x-ray diffraction study of extracellular dermal glycoprotein from carrot and the inhibition complex that it forms with an endo-ß-glucanase from aspergillus aculeatus. | extracellular dermal glycoprotein (edgp) may play an important role in the plant defence system of the carrot (daucus carota) as it has inhibitory activity against endo-ß-glucanase produced by invading pathogens. here, edgp and the inhibition complex that it forms with fi-cmcase, a carboxyl methyl cellulase from aspergillus aculeatus, were successfully crystallized. the hexagonal crystal of edgp belonged to space group p6(2), with unit-cell parameters a = b = 130.4, c = 44.5 å, ? = 120°. the mon ... | 2011 | 21795806 |
| chemopreventive effects of wild carrot oil against 7,12-dimethyl benz(a)anthracene-induced squamous cell carcinoma in mice. | context: daucus carota l. ssp. carota (apiacea) is widely distributed throughout the world and has many uses in traditional medicine. objective: the present study investigates the chemopreventive effects of oil extract of d. carota umbels on 7,12-dimethyl benz(a)anthracene (dmba)-induced skin cancer in mice. materials and methods: d. carota oil extract (dcoe) was prepared by extracting the dried umbels with 50:50 acetone:methanol. skin papilloma were initiated by dmba and promoted by 12-o-tetrad ... | 2011 | 21777042 |
| influence of high-pressure processing on the profile of polyglutamyl 5-methyltetrahydrofolate in selected vegetables. | in plants, folate occurs predominantly as 5-methyltetrahydrofolate (5mthf) polyglutamyl forms. differences in stability and bioavailability of food folate compared to synthetic folic acid have been attributed to the presence of the polyglutamyl chain. high-pressure processing (hpp) was tested for whether it might shorten polyglutamyl chains of 5mthf species in fresh vegetables by enabling action of native γ-glutamylhydrolase (ggh). a validated ultrahigh-performance reversed-phase liquid chromato ... | 2011 | 21770413 |
| quantitative proteomics by 2de and maldi ms/ms uncover the effects of organic and conventional cropping methods on vegetable products. | organic farming aims to be environmentally sound, but the question as to whether organic cropping brings more nutritional benefits to farmers and consumers than the conventional cropping needs still to be answered. to gain insights into the molecular effects of organic farming we used proteome analysis to analyze cabbage (brassica oleracea l. var 'capitata') and carrot (daucus carota var. 'sativus') our aim was to identify the metabolic pathways that are affected by different cropping regimes an ... | 2011 | 21757040 |
| characterisation of polyacetylenes isolated from carrot (daucus carota) extracts by negative ion tandem mass spectrometry. | the potential use of negative electrospray ionisation mass spectrometry (esi-ms) in the characterisation of the three polyacetylenes common in carrots (daucus carota) has been assessed. the ms scans have demonstrated that the polyacetylenes undergo a modest degree of in-source decomposition in the negative ionisation mode while the positive ionisation mode has shown predominantly sodiated ions and no [m+h](+) ions. tandem mass spectrometric (ms/ms) studies have shown that the polyacetylenes foll ... | 2011 | 21735506 |
| molecular systematics of apiaceae subfamily apioideae: phylogenetic analyses of nuclear ribosomal dna internal transcribed spacer and plastid rpo c1 intron sequences. | evolutionary relationships among representatives of apiaceae (umbelliferae) subfamily apioideae have been inferred from phylogenetic analyses of nuclear ribosomal dna internal transcribed spacer (its 1 and its 2) and plastid rpoc1 intron sequences. high levels of nucleotide sequence variation preclude the use of the its region for examining relationships across subfamilial boundaries in apiaceae, whereas the rpoc1 intron is more suitably conserved for family-wide phylogenetic study but is too co ... | 1998 | 21684940 |
| post-dispersal seed fates of four prairie species. | after dispersal, seeds can germinate and establish as seedlings, persist as seeds, or die. knowledge of these three seed fates is crucial for understanding the abundance and distribution of plant populations and ultimately, community composition and diversity. few studies, however, have simultaneously measured these fates, while also examining the factors causing mortality. the goal of this research was to simultaneously quantify the three seed fates and factors causing death (predation and fung ... | 2003 | 21659169 |
| occurrence of clostridium perfringens from different cultivated soils. | the occurrence of clostridium perfringens was estimated in 750 samples originated from a variety of soils bearing various bulb crops: brawnica oderacea (vegetable), olea europaea, daucus carota (carote), solanum tuberosum (potato), phaseolus vulgaris (green haricot), beta vulgaris var. rapaceum (beetroot), cucurbita pepo (squash), allium cepa (onion), cucumis sativus (cucumber) and capsicum annum (pepper). all isolated strains were tested for their antimicrobial activities to amoxicillin, penici ... | 2011 | 21621626 |
| metabolic fingerprinting reveals differences between shoots of wild and cultivated carrot (daucus carota l.) and suggests maternal inheritance or wild trait dominance in hybrids. | differences between the metabolic content of cultivars and their related wild species not only have implications for breeding and food quality, but also for the increasingly studied area of crop to wild introgression. wild and cultivated western carrots belong to the same outcrossing species and hybridize under natural conditions. the metabolic fingerprinting of dutch wild carrot and of western orange carrot cultivar shoots using (1)h nmr showed only quantitative differences in chemical content, ... | 2011 | 21601898 |
| plants as biofactories: physiological role of reactive oxygen species on the accumulation of phenolic antioxidants in carrot tissue under wounding and hyperoxia stress. | plants subjected to postharvest abiotic stresses synthesize secondary metabolites with health-promoting properties. here, we report the potential use of carrots (daucus carota) as biofactories of caffeoylquinic acids when subjected to wounding and hyperoxia stresses. wounding stress induced an increase of ∼287% in total phenolic content (pc) in carrots stored for 48 h at 20 °c. this increase was higher (∼349%) in the wounded tissue treated with hyperoxia stress. to further understand the physiol ... | 2011 | 21553806 |
| comparative fish mapping of daucus species (apiaceae family). | the cytogenetic characterization of the carrot genome (daucus carota l., 2n = 18) has been limited so far, partly because of its somatic chromosome morphology and scant of chromosome markers. here, we integrate the carrot linkage groups with pachytene chromosomes by fluorescent in situ hybridization (fish) mapping genetically anchored bacterial artificial chromosomes (bacs). we isolated a satellite repeat from the centromeric regions of the carrot chromosomes, which facilitated the study of the ... | 2011 | 21547583 |
| dchsp17.7, a small heat shock protein in carrot, is tissue-specifically expressed under salt stress and confers tolerance to salinity. | the expression and function of dchsp17.7, a small heat shock protein in carrot (daucus carota l.), were examined under salt stress, which is an exacerbating environmental condition due to water shortage and irrigation. dchsp17.7 was constitutively expressed in leaf and stem tissues under normal growth conditions. upon exposure to 300mm nacl, the protein level of dchsp17.7 increased dramatically in leaf tissue, but did not significantly change in stem tissue. native-page analysis showed tissue-sp ... | 2011 | 21539946 |
| [qtl mapping for contents of main carotenes and lycopene in carrot (daucus carota l.)]. | an f2 population derived from two carrot inbred lines, p50006 and hcm a.c. with high carotene accumulation, was developed and used to map and analyze quantitative trait locus (qtl) associated with the accumulation of alpha and beta-carotene, total carotene and lycopene. broad-sense heritabilities of these traits were 0.75, 0.50, 0.31, and 0.93, respectively. a genetic map with 91 srap (sequence-related amplified polymorphism) markers was developed, which spanned 502.9 cm in 9 linkage groups with ... | 2010 | 21513156 |
| [spontaneous spectinomycin resistance mutations of the chloroplast rrn16 gene in daucus carota callus lines]. | bioballistic transformation of carrot daucus carota l. callus cultures with a plasmid containing the aada (aminoglycoside 3'-adenyltransferase) gene and subsequent selection oftransformants on a selective medium containing spectinomycin (100-500 mg/l) yielded ten callus lines resistant to this antibiotic. pcr analysis did not detect exogenous dna in the genomes of spectinomycin-resistant calluses. resistance proved to be due to spontaneous mutations that occurred in two different regions of the ... | 2011 | 21446181 |
| differences in protodermal cell wall structure in zygotic and somatic embryos of daucus carota (l.) cultured on solid and in liquid media. | the ultrastructure, cuticle, and distribution of pectic epitopes in outer periclinal walls of protodermal cells of daucus carota zygotic and somatic embryos from solid and suspension culture were investigated. lipid substances were present as a continuous layer in zygotic and somatic embryos cultured on solid medium. somatic embryos from suspension cultures were devoid of cuticle. the ultrastructure of the outer walls of protodermis of embryos was similar in zygotic and somatic embryos from soli ... | 2012 | 21424614 |
| carotene and novel apocarotenoid concentrations in orange-fleshed cucumis melo melons: determinations of β-carotene bioaccessibility and bioavailability. | muskmelons, both cantaloupe (cucumis melo reticulatus group) and orange-fleshed honeydew (c. melo inodorus group), a cross between orange-fleshed cantaloupe and green-fleshed honeydew, are excellent sources of β-carotene. although β-carotene from melon is an important dietary antioxidant and precursor of vitamin a, its bioaccessibility/bioavailability is unknown. we compared β-carotene concentrations from previously frozen orange-fleshed honeydew and cantaloupe melons grown under the same glassh ... | 2011 | 21417375 |
| anthocyanin composition of black carrot (daucus carota ssp. sativus var. atrorubens alef.) cultivars antonina, beta sweet, deep purple, and purple haze. | this study aimed to identify the pigment composition of black carrot (daucus carota ssp. sativus var. atrorubens alef.) cultivars antonina, beta sweet, deep purple, and purple haze. cyanidin 3-xylosyl(glucosyl)galactosides acylated with sinapic acid, ferulic acid, and coumaric acid were detected as major anthocyanins by high-performance liquid chromatography with diode array detection (hplc-dad) and with electrospray ionization multiple mass spectrometry (hplc-esi-ms(n)) analyses. the preparativ ... | 2011 | 21381748 |
| [influence of arbuscular mycorrhizal inoculation on growth and phoxim residue of carrot (daucus carota l.)]. | a pot culture experiment was carried out to study the influence of arbuscular mycorrhizal (am) fungi on the growth and phoxim residue of carrot (daucus carota l). four levels of phoxim (0, 200, 400, 800 mg x l(-1)) and two am fungal inocula, glomus intraradices beg 141(141), glomus mosseae beg 167 (167),and one nonmycorrhizal inoculum (ck), were applied to the sterilized soil. the plants were harvested after 5 months of growth and phoxim was irrigated into the root zone 14 d before plant harvest ... | 2010 | 21360902 |
| essential oils of daucus carota subsp. carota of tunisia obtained by supercritical carbon dioxide extraction. | the essential oils and supercritical co2 extracts of wild daucus carota l. subsp. carota from two different sites in tunisia were investigated. the main components of the essential oil of the flowering and mature umbels with seeds from sejnane were eudesm-7(11)-en-4-ol (8.2 - 8.5%), carotol (3.5 - 5.2%), sabinene (12.0 -14.5%), a-selinene (7.4 - 8.6) and 11-alpha-(h)-himachal-4-en-1-beta-ol (12.7 - 17.4%), whereas the oils from tunis were predominantly composed of elemicin (31.5 - 35.3%) and car ... | 2010 | 21299130 |
| molecular characterization and functional analysis of sucrose-cleaving enzymes in carrot (daucus carota l.). | the amount of carbon transported into storage organs of crop plants to a large degree determines crop yield. the role of sucrose-cleaving enzymes in this process is not clear and it is the main goal of our work to tackle this question. sucrose cleavage is catalysed either by invertase or sucrose synthase both of which exist in several isoforms with different subcellular locations. carrot (daucus carota l.) contains three major isoenzymes of acid invertase, which either accumulate as soluble poly ... | 1996 | 21245247 |
| a study on the effect of few eco-friendly manures on the growth attributes of carrot (daucus carota l.). | a pot culture study was carried out at avinashilingam deemed university, coimbatore to assess the effect of few organic manures (herbal microfertilizer, humic acid, farm yard manure (fym), biofertilizer) and npk on the growth attributes of carrot. among the parameters observed, the germination percentage was highest in fym treated pots on 7 das (days after sowing) and in npk treated pots on 21, 28 and 30 das. the parameters, such as root length, crown length and vigour index, were studied on 30, ... | 2009 | 21114148 |
| effects of ten antibiotics on seed germination and root elongation in three plant species. | we applied a screening-level phytotoxicity assay to evaluate the effects of 10 antibiotics (at concentrations ranging from 1 to 10,000 μg/l) on germination and early plant growth using three plant species: lettuce (lactuca sativa), alfalfa (medicago sativa), and carrot (daucus carota). the range of phytotoxicity of the antibiotics was large, with ec₂₅s ranging from 3.9 μg/l to >10,000 μg/l. chlortetracycline, levofloxacin, and sulfamethoxazole were the most phytotoxic antibiotics. d. carota was ... | 2011 | 21107831 |
| high-level expression of human interferon alpha-2b in transgenic carrot (daucus carota l.) plants. | in this study, we report the obtaining of carrot plants expressing human interferon alpha-2b via agrobacterium-mediated transformation using two vector constructs containing the sequence coding for interferon gene fused with nicotiana plumbagenifolia calreticulin apoplast targeting signal driven by 35s camv promoter and root-specific mll promoter. the human interferon alpha-2b gene was correctly translated in carrot plants according to western blot analysis. the recombinant protein exhibited ant ... | 2010 | 21046110 |
| comparison of two liquid-state nmr methods for the determination of saccharides in carrot (daucus carota l.) roots. | in order to determine the saccharide content of plant tissues, we studied a new non-destructive and fast analytical method that we call "direct quantitative proton nuclear magnetic resonance spectroscopy" (d q (1)h nmr): the application of quantitative proton nuclear magnetic resonance spectroscopy (q (1)h nmr) to non modified plant tissues along with capillary tubes containing a reference compound (for quantification) and deuterium oxide (for locking). using this method, the saccharide content ... | 2011 | 21046086 |
| development and in-house validation of allergen-specific elisa tests for the quantification of dau c 1.01, dau c 1.02 and dau c 4 in carrot extracts (daucus carota). | even though carrot allergy is common in europe, the amount of different allergens in carrots is still unknown due to a lack of methods for quantitative allergen measurements. the current study aimed at the development of quantitative elisa tests for the known carrot allergens, namely dau c 1.01, dau c 1.02, and dau c 4 in pure carrot extracts. monoclonal antibodies targeting the major carrot allergen isoforms dau c 1.01 and dau c 1.02 were generated and combined in sandwich elisa with rabbit ant ... | 2010 | 21046078 |
| effects of harvesting date and storage on the amounts of polyacetylenes in carrots, daucus carota. | the amounts of three main polyacetylenes in carrots; falcarinol, falcarindiol, and falcarindiol-3-acetate, were determined by hplc, during three seasons, in carrots harvested several times per season and at different locations in sweden. the amounts of falcarindiol first decreased from a relatively high level and then increased later in the harvest season. the amounts of falcarindiol-3-acetate showed similar variations, whereas the amounts of falcarinol did not exhibit any significant variation ... | 2010 | 20964297 |
| ambispora granatensis, a new arbuscular mycorrhizal fungus, associated with asparagus officinalis in andalucia (spain). | a new dimorphic fungal species in the arbuscular mycorrhiza-forming glomeromycota, ambispora granatensis, was isolated from an agricultural site in the province of granada (andalucía, spain) growing in the rhizosphere of asparagus officinalis. it was propagated in pot cultures with trifolium pratense and sorghum vulgare. the fungus also colonized ri t-dna transformed daucus carota roots but did not form spores in these root organ cultures. the spores of the acaulosporoid morph are 90-150 μm diam ... | 2011 | 20952800 |
| arbuscular mycorrhizal hyphopodia and germinated spore exudates trigger ca2+ spiking in the legume and nonlegume root epidermis. | • the aim of this study was to investigate ca(2+) responses to endosymbiotic arbuscular mycorrhizal (am) fungi in the host root epidermis following pre-infection hyphopodium formation in both legumes and nonlegumes, and to determine to what extent these responses could be mimicked by germinated fungal spore exudate. • root organ cultures of both medicago truncatula and daucus carota, expressing the nuclear-localized cameleon reporter nupyc2.1, were used to monitor am-elicited ca(2+) responses in ... | 2011 | 20880223 |
| association of "candidatus liberibacter solanacearum" with the psyllid, trioza apicalis (hemiptera: triozidae) in europe. | the psyllid trioza apicalis förster (hemiptera: triozidae) is a serious pest of carrots, daucus carota l., in europe. carrots exhibiting symptoms of psyllid damage were observed in commercial fields in southern finland in 2008. symptoms in affected plants included leaf curling, yellow and purple discoloration of leaves, stunted growth of shoots and roots, and proliferation of secondary roots. mechanisms by which t. apicalis induces symptoms in plants are not understood, and no plant pathogens ha ... | 2010 | 20857712 |
| effect of carrot (daucus carota) microstructure on carotene bioaccessibility in the upper gastrointestinal tract. 2. in vivo digestions. | nutrient bioaccessibility and subsequent absorption will be directly influenced by changes in food structure during gastrointestinal processing. the accompanying paper (tydeman et al. j. agric. food chem. 2010, 58, doi: 10.1021/jf101034a) reported results on the effect of carrot processing on the release of carotene into lipid phases during in vitro gastric and small intestinal digestions. this paper describes results from in vivo digestion of two of the types of processed carrot used previously ... | 2010 | 20806973 |
| enhanced disease resistance in transgenic carrot (daucus carota l.) plants over-expressing a rice cationic peroxidase. | plant class iii peroxidases are involved in numerous responses related to pathogen resistance including controlling hydrogen peroxide (h(2)o(2)) levels and lignin formation. peroxidases catalyze the oxidation of organic compounds using h(2)o(2) as an oxidant. we examined the mechanisms of disease resistance in a transgenic carrot line (p23) which constitutively over-expresses the rice cationic peroxidase osprx114 (previously known as po-c1) and which exhibits enhanced resistance to necrotrophic ... | 2010 | 20730544 |
| effect of carrot (daucus carota) microstructure on carotene bioaccessibilty in the upper gastrointestinal tract. 1. in vitro simulations of carrot digestion. | studies investigating carotene bioaccessibility (release from the food matrix to a solubilized form) directly from plant material during the process of digestion are scarce, mainly due to the difficulties associated with obtaining such material. therefore, this paper examines the relationship between tissue microstructure and carotene bioaccessibility using an in vitro digestion model. dietary oil provides a pool for the initial solubilization. therefore, carotene partitioning into an emulsified ... | 2010 | 20698537 |
| historical and contemporary gene dispersal in wild carrot (daucus carota ssp. carota) populations. | wild carrot is the ancestor of cultivated carrot and is the most important gene pool for carrot breeding. transgenic carrot may be released into the environment in the future. the aim of the present study was to determine how far a gene can disperse in wild carrot populations, facilitating risk assessment and management of transgene introgression from cultivated to wild carrots and helping to design sampling strategies for germplasm collections. | 2010 | 20566679 |
| nutritional, physical, and sensory evaluation of hydroponic carrots (daucus carota l.) from different nutrient delivery systems. | carrot (daucus carota l.) has the highest carotenoid content among foods and is consumed in large quantities worldwide, while at the same time its market demand continues to increase. carotenoids have also been associated with protective effects against cancer and other chronic diseases. the most predominant carotenoids in carrots are beta- and alpha-carotenes. moisture, ash, fat, texture, color, carotene content, and consumer acceptance of carrots grown in a hydroponic system with nutrient film ... | 2013 | 20492130 |
| differential effect of sample preservation methods on plant and arbuscular mycorrhizal fungal dna. | a wide range of methods are commonly used for preserving environmental samples prior to molecular analyses. however, the effect of these preservation methods on fungal dna is not understood. the objective of this study was to test the effect of eight different preservation methods on the quality and yield of dna extracted from bromus inermis and daucus carota roots colonized by the arbuscular mycorrhizal (am) fungus, glomus intraradices. the total dna concentration in sample extracts was quantif ... | 2010 | 20470836 |
| obtaining carrot (daucus carota l.) plants in isolated microspore cultures. | microspores were cultured on the modified b5 liquid medium containing 2.4d (0.1 mg l(-1)), naa (0.1 mg l(-1)), l-glutamine (500 mg l(-1), l-serine (100 mg l(-1)), and sucrose (100 g l(-1)). the developmental stages of microspores and divisions were observed. initially, the formation of binuclear and multicellular structures was noticed. plants regenerated in the cultures in which the tetrad stage of microsporogenesis had predominated. embryoids were still forming 24 weeks after the cultures were ... | 2010 | 20453301 |
| carotenoid biosynthesis genes provide evidence of geographical subdivision and extensive linkage disequilibrium in the carrot. | according to the history of the cultivated carrot, root colour can be considered as a structural factor of carrot germplasm. therefore, molecular variations of carotenoid biosynthesis genes, these being involved in colour traits, represent a good putative source of polymorphism related to diversity structure. seven candidate genes involved in the carotenoid biosynthesis pathway have been analysed from a sample of 48 individual plants, each one from a different cultivar of carrot (daucus carota l ... | 2010 | 20411232 |
| effectiveness of different edu concentrations in ameliorating ozone stress in carrot plants. | ethylenediurea (edu) is suggested for use to evaluate plant response under ambient ozone (o(3)) concentrations. four edu treatments, viz. 0 (non-edu), 150, 300 and 450 mg l(-1), applied as soil drench at 10 days interval to carrot (daucus carota l. var. pusa kesar), grown at a tropical suburban site of varanasi experiencing mean o(3) concentration of 36.1 ppb during the experimental period. edu treated plants showed significantly higher antioxidative defense, assimilation capability and reduced ... | 2010 | 20403638 |
| deposition of carotenoids in egg yolk by short-term supplement of coloured carrot (daucus carota) varieties as forage material for egg-laying hens. | supplying egg-laying hens with different forage materials may influence egg production and quality. the aim of this study was to examine the short-term effects of standard feed plus 70 g day(-1) per hen of three coloured carrot varieties (orange, yellow and purple) as forage material in comparison with a standard feed control on egg production, egg yolk colour and deposition of carotenoids in the yolk. | 2010 | 20393997 |
| effect of storage and cooking on beta-carotene isomers in carrots ( daucus carota l. cv. 'stefano'). | carrots are one of the highest dietary sources of beta-carotene and are naturally high in the (all-e)-beta-carotene isomer, which has higher bioavailability, provitamin a activity, and antioxidant capacity compared to z (cis) isomers. the objectives of the present study were to investigate the effects of storage temperature, time, and cooking (boiling for 15 min) on the levels of carotene isomers in 'stefano' carrots. storing carrots at either 4 degrees c to simulate long-term storage or 20 degr ... | 2010 | 20359248 |
| influence of thermal processing on hydrolysis and stability of folate poly-gamma-glutamates in broccoli (brassica oleracea var. italica), carrot (daucus carota) and tomato (lycopersicon esculentum). | the folate poly-gamma-glutamate profile, their concentrations, and hydrolysis by endogenous gamma-glutamyl hydrolase (ggh) were evaluated in broccoli, carrot and tomato. further studies on the effect of time and temperature on folate poly-gamma-glutamate hydrolysis and stability were carried out in broccoli since this vegetable showed the highest long-chain and total folate poly-gamma-glutamate concentration. the evolution of l-ascorbic acid, total phenols and trolox equivalent antioxidant capac ... | 2010 | 20329724 |
| one new guaiane-type sesquiterpene glycoside from the fruits of daucus carota l. | a new guaiane-type sesquiterpene glycoside, torilolone 8-o-beta-d-glucopyranoside (1), together with a known analogue compound, torilolone 11-o-beta-d-glucopyranoside (2), was isolated from the fruits of daucus carota l. their chemical structures were elucidated on the basis of ms, nmr spectroscopic analyses coupled with chemical degradation. the cytotoxic activities of two isolated compounds were evaluated against two human gastric cancer cell lines bgc-823 and ags using mtt assay. | 2010 | 20187582 |
| anthocyanins in purple-orange carrots (daucus carota l.) do not influence the bioavailability of beta-carotene in young women. | purple carrots contain anthocyanins in addition to the provitamin a carotenoids in typical orange carrots. simultaneous consumption of these phytochemicals in carrots may affect the bioavailability of carotenoids. the bioavailability of beta-carotene in humans was assessed from an acute feeding of orange (oc) and purple (pc) carrots with white (wc) as a control. carrot smoothies were served to female subjects (n = 5, aged 21-26 years) for breakfast after 1 week on a low carotenoid diet and overn ... | 2010 | 20131807 |
| specific developmental pathways underlie host specificity in the parasitic plant orobanche. | parasitic angiosperms are an ecologically and economically important group of plants. however our understanding of the basis for host specificity in these plants is embryonic. recently we investigated host specificity in the parasitic angiosperm orobanche minor, and demonstrated that this host generalist parasite comprises genetically defined races that are physiologically adapted to specific hosts. populations occurring naturally on red clover (trifolium pratense) and sea carrot (daucus carota ... | 2010 | 20081361 |
| two new guaiane-type sesquiterpenoids from the fruits of daucus carota l. | two new guaiane-type sesquiterpenoids containing an interesting epoxy unit, daucuside (1) and daucusol (2) were isolated from the fruits of daucus carota l. their chemical structures were elucidated on the basis of ms, nmr spectroscopic analyses coupled with chemical degradation and they were also evaluated for the cytotoxic effects against two human gastric cancer cell lines bgc-823 and ags. | 2010 | 20060880 |
| two new guaiane-type sesquiterpene glycosides from the fruits of daucus carota l. | two new guaiane-type sesquiterpene glycosides, 11-o-acetyl-torilolone 8-o-beta-d-glucopyranoside (1) and 1beta-hydroxytorilolone 11-o-beta-d-glucopyranoside (2), were isolated from the fruits of daucus carota l. their chemical structures were elucidated on the basis of ms, nmr spectroscopic analyses coupled with chemical degradation. | 2010 | 20045981 |
| differential nuclear envelope assembly at the end of mitosis in suspension-cultured apium graveolens cells. | nmcp1 is a plant protein that has a long coiled-coil domain within the molecule. newly identified nmcp2 of daucus carota and apium graveolens showed similar peripheral localization in the interphase nucleus, and the sequence spanning the coiled-coil domain exhibited significant similarity with the corresponding region of nmcp1. to better understand disassembly and assembly of the nuclear envelope (ne) during mitosis, subcellular distribution of nmcp1 and nmcp2 was examined using a. graveolens ce ... | 2010 | 19997923 |
| polyacetylene diversity and bioactivity in orange market and locally grown colored carrots (daucus carota l.). | carrots contain a wide array of phytochemicals such as carotenoids, phenolics, alpha-tocopherol, and polyacetylenes. carrots are most known for their pro-vitamin a carotenoids but also contain other phytochemicals with documented health benefits. the phytochemicals in colored carrots present a challenge and opportunity due to the wide diversity of potent bioactive compounds. two commercial carrots, 1 wild carrot, and 13 colored carrot varieties were characterized phytochemically. the carrots wer ... | 2009 | 19950995 |
| carrot alternative oxidase gene aox2a demonstrates allelic and genotypic polymorphisms in intron 3. | single nucleotide polymorphisms (snps) and insertion-deletions (indels) are becoming important genetic markers for major crop species. in this study, we focus on variations at genomic level of the daucus carota l. aox2a gene. the use of gene-specific primers designed in exon regions on the boundaries of introns permitted to recognize intron length polymorphism (ilp) in intron 3 aox2a by simple polymerase chain reaction (pcr) assays. the length of intron 3 can vary in individual carrot plants. th ... | 2009 | 19941625 |
| structure of the major carrot allergen dau c 1. | dau c 1 is a major allergen of carrot (daucus carota) which displays ige cross-reactivity with the homologous major birch-pollen allergen bet v 1. the crystal structure of dau c 1 has been determined to a resolution of 2.7 a, revealing tight dimers. the structure of dau c 1 is similar to those of the major allergens from celery, api g 1, and birch pollen, bet v 1. electron density has been observed in the hydrophobic cavity of each monomer and has been modelled with polyethylene glycol oligomers ... | 2009 | 19923716 |
| quantitative permeability imaging of plant tissues. | a method for mapping tissue permeability based on time-dependent diffusion measurements is presented. a pulsed field gradient sequence to measure the diffusion encoding time dependence of the diffusion coefficients based on the detection of stimulated spin echoes to enable long diffusion times is combined with a turbo spin echo sequence for fast nmr imaging (mri). a fitting function is suggested to describe the time dependence of the apparent diffusion constant in porous (bio-)materials, even if ... | 2010 | 19921172 |
| response to uv-c radiation in topo i-deficient carrot cells with low ascorbate levels. | in animal cells, recent studies have emphasized the role played by dna topoisomerase i (topo i) both as a cofactor of dna repair complexes and/or as a damage sensor. all these functions are still unexplored in plant cells, where information concerning the relationships between dna damage, pcd induction, and topo i are also limited. the main goal of this study was to investigate the possible responses activated in topo i-depleted plant cells under oxidative stress conditions which induce dna dama ... | 2010 | 19917599 |
| alternative oxidase involvement in daucus carota somatic embryogenesis. | plant alternative oxidase (aox) is a mitochondrial inner membrane enzyme involved in alternative respiration. the critical importance of the enzyme during acclimation upon stress of plant cells is not fully understood and is still an issue of intensive research and discussion. recently, a role of aox was suggested for the ability of plant cells to change easily its fate upon stress. in order to get new insights about aox involvement in cell reprogramming, quantitative real-time polymerase chain ... | 2009 | 19863756 |
| broad-spectrum disease resistance to necrotrophic and biotrophic pathogens in transgenic carrots (daucus carota l.) expressing an arabidopsis npr1 gene. | the development of transgenic plants highly resistant to a range of pathogens using traditional signal gene expression strategies has been largely ineffective. modification of systemic acquired resistance (sar) through the overexpression of a controlling gene such as npr1 (non-expressor of pr genes) offers an attractive alternative for augmenting the plants innate defense system. the arabidopsis (at) npr1 gene was successfully introduced into 'nantes coreless' carrot under control of a camv 35s ... | 2009 | 19859731 |
| structure determination of bisacetylenic oxylipins in carrots (daucus carota l.) and enantioselective synthesis of falcarindiol. | although bisacetylenic oxylipins have been demonstrated to exhibit diverse biological activities, the chemical structures of many representatives of this class of phytochemicals still remain elusive. as carrots play an important role in our daily diet and are known as a source of bisacetylenes, an extract made from daucus carota l. was screened for bisacetylenic oxylipins, and, after isolation, their structures were determined by means of lc-ms and 1d/2d nmr spectroscopy. besides the previously ... | 2009 | 19845355 |
| gene transcription analysis of carrot allergens by relative quantification with single and duplex reverse transcription real-time pcr. | single and duplex real-time polymerase chain reaction (pcr) systems have been developed to quantify specific mrna transcription of genes coding for the major daucus carota allergen isoforms dau c 1.01 and dau c 1.02. methods were tested with samples from the local market. whereas the gene transcription levels for dau c 1.01 were consistently high in all investigated samples, significant differences for the dau c 1.02 transcription could be demonstrated in randomly collected market samples. the g ... | 2010 | 19834694 |
| differential expression and co-regulation of carrot aox genes (daucus carota). | alternative oxidase (aox) is a mitochondrial protein encoded by the nuclear genome. in higher plants aox genes form a small multigene family mostly consisting of the two subfamilies aox1 and aox2. daucus carota l. is characterized by a unique extension pattern of aox genes. different from other plant species studied so far it contains two genes in both subfamilies. therefore, carrot was recently highlighted as an important model in aox stress research to understand the evolutionary importance of ... | 2009 | 19825008 |
| splinkbes: a splinkerette-based method for generating long end sequences from large insert dna libraries. | we report on the development of a novel splinkerette-based method for generating long end sequences from large insert library clones, using a carrot (daucus carota l.) bac library as a model. the procedure involves digestion of the bac dna with a 6-bp restriction enzyme, followed by ligation of splinkerette adaptors that serve as primer-annealing sites for pcr amplification of the bac ends. the resulting amplicons are sequenced from both directions, and when overlapping, the pairs of sequences a ... | 2009 | 19737131 |
| transmission of different isolates of spiroplasma citri to carrot and citrus by circulifer tenellus (hemiptera: cicadellidae). | carrot purple leaf disease was first reported in 2006 in the state of washington and was associated with spiroplasma citri. the disease also was reported in california in 2008. the objectives of this work were to fulfill koch's postulates and to determine 1) whether the beet leafhopper, circulifer tenellus (baker) (hemiptera: cicadellidae), transmits carrot [daucus carota l. subsp. sativus (hoffm.) arcang] isolates of s. citri; and 2) whether carrot and citrus [citrus sinensis (l.) osb.]-derived ... | 2009 | 19736751 |
| proteomics as a way to identify extra-radicular fungal proteins from glomus intraradices- rit-dna carrot root mycorrhizas. | to identify fungal proteins involved in the arbuscular mycorrhizal symbiosis, root-inducing transferred-dna transformed roots of carrot (daucus carota l.) were in vitro inoculated with glomus intraradices. proteins extracted from the extra-radical fungus were analysed by two-dimensional gel electrophoresis. a fungal reference map displaying 438 spots was set up. four proteins, among the 14 selected for tandem mass spectrometry analysis, were identified including a nmra-like protein, an oxido-red ... | 2004 | 19712309 |
| a new sesquiterpene from the fruits of daucus carota l. | phytochemical investigation of the fruits of daucus carota l. resulted in the isolation of a new sesquiterpene named as daucucarotol (1). its structure was elucidated on the basis of 1d and 2d nmr experiments, coupled with ms studies. to our knowledge, compound 1 is the first example for a natural eudesmane sesquiterpene with a hydroxymethyl group located at a methine carbon rather than a usual quaternary carbon in the two fused six-membered ring systems. | 2009 | 19701130 |
| genetic and physiological determinants of streptomyces scabies pathogenicity. | summary common scab is a severe disease worldwide affecting tap root crops and potato tubers. it is caused by soil-borne filamentous bacteria belonging to the genus streptomyces. streptomycetes usually are saprophytic microorganisms, but a few species have acquired the ability to infect underground plant tissues. the predominant causal agent of potato scab worldwide is streptomyces scabies. the production of phytotoxins called thaxtomins is essential for the virulence of common scab-causing agen ... | 2009 | 19694949 |
| development and evaluation of monoclonal antibodies as probes to assess the differences between two tomato pectin methylesterase isoenzymes. | the enzyme pectin methylesterase (pme) was purified from red ripe tomatoes (lycopersicon esculentum) and through affinity chromatography two isoenzymes were fractionated (t1pme and t2pme). further analysis of these two isoenzymes, both having a molar mass of 34.5kda, revealed a difference in the n-terminal sequence and in amino acid composition. t1pme was identified as the major isoenzyme of pme in tomato fruit. in this study the aim was to develop a toolbox, consisting of monoclonal antibodies, ... | 2009 | 19686752 |
| overexpressing atpap15 enhances phosphorus efficiency in soybean. | low phosphorus (p) availability is a major constraint to crop growth and production, including soybean (glycine max), on a global scale. however, 50% to 80% of the total p in agricultural soils exists as organic phosphate, which is unavailable to plants unless hydrolyzed to release inorganic phosphate. one strategy for improving crop p nutrition is the enhanced activity of acid phosphatases (apases) to obtain or remobilize inorganic phosphate from organic p sources. in this study, we overexpress ... | 2009 | 19587103 |
| flower and root oils of the tunisian daucus carota l. ssp. maritimus (apiaceae): integrated analyses by gc, gc/ms, and 13c-nmr spectroscopy, and in vitro antibacterial activity. | the essential oils from flowers and roots of daucus carota l. ssp. maritimus were obtained by hydrodistillation and analyzed by combination of gc, gc/ms, and (13)c-nmr. the chemical composition of the flower and root oils of this subspecies is reported here for the first time. thirty-two and six compounds were identified in flower and root oils, respectively. a remarkable difference was found between the constituent percentages of the two organs. the chemical composition of the essential oil fro ... | 2009 | 19551729 |
| identification and characterization of bzip-type transcription factors involved in carrot (daucus carota l.) somatic embryogenesis. | seed dormancy is an important adaptive trait that enables seeds of many species to remain quiescent until conditions become favorable for germination. abscisic acid (aba) plays an important role in these developmental processes. like dormancy and germination, the elongation of carrot somatic embryo radicles is retarded by sucrose concentrations at or above 6%, and normal growth resumes at sucrose concentrations below 3%. using a yeast one-hybrid screening system, we isolated two bzip-type transc ... | 2009 | 19519801 |
| suboptimal temperature favors reserve formation in biennial carrot (daucus carota) plants. | in response to suboptimal temperatures, temperate annual plants often increase root:shoot ratios, build-up carbohydrates and display typical morphological and anatomical changes. we know less about the responses of biennials such as carrot. as a model plant, carrot has the additional feature of two functionally and morphologically distinct root parts: the taproot, which stores carbohydrate and other compounds, and the fibrous root system involved in acquisition of water and nutrients. here, we a ... | 2009 | 19493310 |
| expression of rabies virus g protein in carrots (daucus carota). | antigens derived from various pathogens can readily be synthesized at high levels in plants in their authentic forms. such antigens administered orally can induce an immune response and, in some cases, result in protection against a subsequent challenge. we here report the expression of rabies virus g protein into carrots. the g gene was subcloned into the pucpssrabg vector and then used to transform carrot embryogenic cells by particle bombardment. the carrot cells were selected in liquid mediu ... | 2009 | 19479338 |
| daucus carota as a novel model to evaluate the effect of light on carotenogenic gene expression. | carotenoids are synthesized in prokaryotic and eukaryotic organisms. in plants and algae, these lipophilic molecules possess antioxidant properties acting as reactive oxygen species scavengers and exert functional roles in hormone synthesis, photosynthesis, photomorphogenesis and in photoprotection. during the past decade almost all carotenogenic genes have been identified as a result of molecular, genetic and biochemical approaches utilizing arabidopsis thaliana as the model system. studies car ... | 2008 | 19399342 |
| host-driven divergence in the parasitic plant orobanche minor sm. (orobanchaceae). | many parasitic angiosperms have a broad host range and are therefore considered to be host generalists. orobanche minor is a nonphotosynthetic root parasite that attacks a range of hosts from taxonomically disparate families. in the present study, we show that o. minor sensu lato may comprise distinct, genetically divergent races isolated by the different ecologies of their hosts. using a three-pronged approach, we tested the hypothesis that intraspecific taxa o. minor var. minor and o. minor ss ... | 2008 | 19378406 |
| daucus carota l.--an old model for cell reprogramming gains new importance through a novel expansion pattern of alternative oxidase (aox) genes. | the paper highlights daucus carota l. as an ideal model to complement plant stress research on arabidopsis thaliana l. recently, alternative oxidase (aox) is discussed as functional marker candidate for cell reprogramming upon stress. carrot is the most studied species for cell reprogramming and our current research reveals that it is the only one that has expanded both aox sub-family genes. we point to recently published, but not discussed results on conserved differences in the vicinity of the ... | 2009 | 19372042 |
| antioxidant phytochemicals and antioxidant capacity of biofortified carrots (daucus carota l.) of various colors. | antioxidants and antioxidant capacity of seven colored carrots were determined. five anthocyanins, chlorogenic acid, caffeic acid, and four carotenoids were quantified by hplc. total phenolic content was determined according to the folin-ciocalteu method. antioxidant capacities of the hydrophilic and hydrophobic fractions were determined by using the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (abts) and 2,2'-diphenyl-1-picrylhydrazyl (dpph) methods. the relative antioxidant capacity i ... | 2009 | 19358535 |
| accumulation of p-hydroxybenzoic acid in hairy roots of daucus carota 2: confirming biosynthetic steps through feeding of inhibitors and precursors. | biosynthesis of hydroxybenzoates even at enzymatic level is poorly understood. in this report, effect of feeding of putative biosynthetic precursors and pathway-specific enzyme inhibitors of early phenylpropanoid pathway on p-hydroxybenzoic acid accumulation in chitosan-elicited hairy roots of daucus carota was studied. three selective metabolic inhibitors of plant phenylpropanoid pathway, namely, aminooxyacetic acid (aoaa), piperonylic acid (pip) and 3,4-methylenedioxycinnamic acid (mdca), whic ... | 2009 | 19342120 |
| ontogeny of daucus carota infected with meloidogyne hapla. | the ontogeny of carrots (daucus carota cv. 'spartan premium') grown under greenhouse conditions in pots of organic soil infected with meloidogyne hapla was influenced detrimentally as early as 4 days after seeding, as determined through analysis of plant surface area, dry weight, fresh weight, net assimilation rate, relative growth rate, and leaf-area ratio. only 58% of the diseased carrots were suitable for fresh market, compared with 97% of those grown in nematode-free soil. growth and develop ... | 1978 | 19305837 |
| varietal response and estimates of heritability of resistance to meloidogyne javanica in carrots. | with methods developed in this study, varietal responses to m. javanica were evaluated and heritability of resistance of two promising carrot cultivars was estimated. more egg masses were found on root systems inoculated with eggs added to the soil in three holes in 250 cm(3) cups than by mixing the inoculum with soil in the cups. a resistant breeding line, cnph 1437, was discriminated from susceptible cultivar nova kuroda with inoculum levels higher than 2,000 eggs per cup. greenhouse and field ... | 1986 | 19294218 |
| penetration, development, reproduction, and sex ratio of meloidogyne javanica in three carrot cultivars. | 1986 | 19294200 | |
| cropping effects of marigolds, corn, and okra on population levels of meloidogyne javanica and on carrot yields. | 1984 | 19294045 | |
| effect of combining soil solarization with certain nematicides on target and nontarget organisms and plant growth. | field experiments compared pesticidal and plant growth effects of soil solarization, alone and in combination, with overall applications of several nematicides. nematodes, including meloidogyne incognita j2, that were targeted for control were significantly reduced (p < 0.05) by solarization, 1,3-dichloropropene (44 and 132 liter/ha), ethoprop (13.5 kg/ha), metham sodium (64 liter/ha), formaldehyde (111 liter/ha), and by solarization-nematicide combinations. control of pythium ultimum also was o ... | 1987 | 19290287 |
| growth of carrot and tomato from oxamyl-coated seed and control of meloidogyne hapla. | oxamyl was coated on carrot (daucus carota l. cv. spartan fancy-80) and tomato (lycopersicon esculentum mill. cv. glamour) seeds with a polymer sticker for the control of meloidogyne hapla. the sticker diluted in water 1:1 delayed carrot seedling emergence. oxamyl at 40 mg/ml in a 1:5 dilution of sticker lowered the rate of carrot seedling emergence until day 13 and plant growth until day 28. oxamyl at 20 or 40 mg/ml in a 1:5 dilution of sticker on carrot seeds planted in m. hapla-infested muck ... | 1990 | 19287706 |
| distribution of heterodera carotae and meloidogyne hapla in michigan carrot production. | during 1986 and 1988, selected farms in all of the major carrot-growing counties of michigan were surveyed to determine the extent of infestation by heterodera carotae and meloidogyne hapla. both species were found in all eight counties surveyed, but not on all farms. heterodera carotae was recovered from 67.4% of the fields surveyed. meloidogyne hapla was detected in 24.8% of the samples and from 69.8% of the fields. in most cases, h. carotae and m. hapla occurred in the same field. | 1992 | 19283061 |
| effects of cropping sequences on population densities of meloidogyne hapla and carrot yield in organic soil. | the influence of various cropping sequences on population densities of meloidogyne hapla and carrot yield was studied in organic soil under microplot-and field conditions. spinach, radish, barley, oat, and wheat were poor or nonhosts for m. hapla. population densities of m. hapla were maintained or increased on cabbage, celery, lettuce, leek, marigold, and potato. marketable percent-age and root weight of carrots were greater following spinach, oat, radish, and fallow-onion than those following ... | 1992 | 19283022 |
| damage to italian crops caused by cyst-forming nematodes. | investigations were undertaken in 1982-88 to estimate yield losses of carrot, sugarbeet, wheat, and potato caused by cyst-forming nematodes (heterodera and globodera species) in several provinces of italy. soil samples were collected at planting in 0.5-ha sampling areas distributed in each crop's major production area. yield loss estimates were based on nematode population estimates and on curves derived earlier relating nematode densities with crop yields in italy. estimated yield loss values w ... | 1993 | 19279850 |
| survey of crop losses in response to phytoparasitic nematodes in the united states for 1994. | previous reports of crop losses to plant-parasitic nematodes have relied on published results of survey data based on certain commodities, including tobacco, peanuts, cotton, and soybean. reports on crop-loss assessment by land-grant universities and many commodity groups generally are no longer available, with the exception of the university of georgia, the beltwide cotton conference, and selected groups concerned with soybean. the society of nematologists extension committee contacted extensio ... | 1999 | 19270925 |
| a pathotype system to describe intraspecific variation in pathogenicity of meloidogyne chitwoodi. | tests of eight dutch meloidogyne chitwoodi isolates to the differential set for host races 1 and 2 in m. chitwoodi provided no evidence for the existence of host race 2 in the netherlands. the data showed deviations from expected reactions on the differential hosts, which raised doubts of the usefulness of the host race classification in m. chitwoodi. the term ''pathotype'' is proposed for groups of isolates of one meloidogyne sp. that exhibit the same level of pathogenicity on genotypes of one ... | 1999 | 19270911 |
| possible involvement of dna methylation on expression regulation of carrot lec1 gene in its 5'-upstream region. | dna methylation plays important roles in various developmental processes in many organisms. in carrots, the treatment of embryogenic cells (ecs) with dna methylation inhibitors induces hypomethylation and blocks somatic embryogenesis. carrot-leafy cotyledon 1 (c-lec1) is an important transcription factor for embryo development that shows embryo-specific expression in ecs and somatic and zygotic embryos. however, the regulation of embryo-specific transcription factor genes such as c-lec1 in plant ... | 2009 | 19264116 |
| host-specific races in the holoparasitic angiosperm orobanche minor: implications for speciation in parasitic plants. | orobanche minor is a root-holoparasitic angiosperm that attacks a wide range of host species, including a number of commonly cultivated crops. the extent to which genetic divergence among natural populations of o. minor is influenced by host specificity has not been determined previously. here, the host specificity of natural populations of o. minor is quantified for the first time, and evidence that this species may comprise distinct physiological races is provided. | 2009 | 19251714 |
| effect of home freezing and italian style of cooking on antioxidant activity of edible vegetables. | in this study, we analyzed the modifications of antioxidant activity consequent to 3 typical home cooking practices (steaming, boiling, and microwave cooking) in fresh and home frozen vegetables. six different vegetable species were examined: carrots (daucus carota l.), zucchini (cucurbita pepo l.), tomatoes (solanumn lycopersicum l.), green beans (phaseolus vulgaris l.), peas (pisum sativum l.), and yellow peppers (capsicum annuum l.). all vegetables were conventional products and were analyzed ... | 2008 | 19241586 |
| daucus carota mediated-reduction of cyclic 3-oxo-amines. | carrots (daucus carota) were used to reduce cyclic amino-ketones in high yields and enantiomeric excesses. this cheap, eco-compatible, and efficient reducing reagent allows the easy access to precursors of biologically active products. | 2009 | 19215083 |
| ginsenoside rb1 in asymmetric somatic hybrid calli of daucus carota with panax quinquefolius. | american ginseng (panax quinquefolius l.) is one of the most valuable herbs in the world. its major active components are ginsenosides. in order to produce ginsenoside heterogeneously, somatic hybridization, a novel approach for genetic introgression, was employed in this study. protoplasts derived from respective calli of carrot (daucus carota var. sativus hoffm.) and american ginseng (p. quinquefolius l.) were used as the fusion partners. hybrid calli derived from single cell lines containing ... | 2009 | 19199101 |
| production of the p24 capsid protein from hiv-1 subtype c in arabidopsis thaliana and daucus carota using an endoplasmic reticulum-directing sekdel sequence in protein expression constructs. | an optimized gene expression construct was designed in order to increase the accumulation of the hiv-1 subtype c p24 protein in arabidopsis thaliana and carrot (daucus carota) plants. an er retention signal was introduced into the genetic construct generating a p24 protein containing a sekdel amino acid sequence at its c-terminus. mature a. thaliana plants and carrot cells were transformed using agrobacterium tumefaciens carrying the improved pgreen0229/p24_sekdel vector. several transgenic plan ... | 2009 | 19167502 |
| molecular characterization of two alphacryptovirus dsrnas isolated from daucus carota. | 2009 | 19165417 | |
| effect of wild flowers on oviposition of hippodamia variegata (coleoptera: coccinellidae) in the laboratory. | marginal vegetation in crops is very important for natural enemies and their pest control capacity. the effects of brassica nigra l. (brassicaceae), daucus carota l. (apiaceae), and sonchus oleraceous l. (asteraceae) flowers as supplemental food on the number of eggs laid during 7 d and on the preoviposition time in hippodamia variegata (goeze, 1777) were studied in the laboratory under conditions of several densities of acyrthosiphon pisum (harris, 1776). the results show the presence of flower ... | 2008 | 19133458 |
| characterization of a deep-coverage carrot (daucus carota l.) bac library and initial analysis of bac-end sequences. | carrot is the most economically important member of the apiaceae family and a major source of provitamin a carotenoids in the human diet. however, carrot molecular resources are relatively underdeveloped, hampering a number of genetic studies. here, we report on the synthesis and characterization of a bacterial artificial chromosome (bac) library of carrot. the library is 17.3-fold redundant and consists of 92,160 clones with an average insert size of 121 kb. to provide an overview of the compos ... | 2009 | 19104839 |