Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
sulfenylated proteins in the medicago truncatula-sinorhizobium meliloti symbiosis.reactive oxygen species such as hydrogen peroxide (h(2)o(2)), play a crucial role as signaling molecules in the establishment and functioning of the nitrogen-fixing legume-rhizobium symbiosis. the regulation of protein function through oxidative modification has emerged as an important molecular mechanism modulating various biological processes. protein cysteine residues are known to be sensitive targets of h(2)o(2), in a posttranslational modification called sulfenylation. we trapped and identi ...201222634402
putative use of a bacillus subtilis l194 strain for biocontrol of phoma medicaginis in medicago truncatula seedlings.an antagonist l194 strain against phoma medicaginis pathogenic fungi was isolated from tunisian soil (vicinity of tunis) and identified as bacillus subtilis based on biochemical characteristics and partial 16s rdna sequence. when cells were grown in a minimal medium for 24 h, spore culture supernatant exhibited 2-fold higher antifungal activity than vegetative cells. maldi-tof mass spectrometry analysis showed that l194 spores produced mainly iturins, surfactins and fengycins with long-chain fat ...201222579659
transcriptional responses to drought stress in root and leaf of chickpea seedling.chickpea (cicer arietinum l.) is an important pulse crop grown mainly in the arid and semi-arid regions of the world. due to its taxonomic proximity with the model legume medicago truncatula and its ability to grow in arid soil, chickpea has its unique advantage to understand how plant responds to drought stress. in this study, an oligonucleotide microarray was used for analyzing the transcriptomic profiles of unigenes in leaf and root of chickpea seedling under drought stress, respectively. mic ...201222562393
tnt1 retrotransposon tagging of stf in medicago truncatula reveals tight coordination of metabolic, hormonal and developmental signals during leaf morphogenesis.tnt1 (transposable element if nicotiana tabaccum cell type 1) is one of the very few active ltr retrotransposons used for gene tagging in plants. in the model legume medicago truncatula, tnt1 has been effectively used as a gene knock-out tool to generate several very useful mutants. stenofolia (stf) is such a mutant identified by tnt1 insertion in a wuschel-like homeobox transcription factor. stf is required for blade outgrowth, leaf vascular patterning and female reproductive organ development ...201122545243
high toxicity and specificity of the saponin 3-glca-28-ararhaxyl-medicagenate, from medicago truncatula seeds, for sitophilus oryzae.because of the increasingly concern of consumers and public policy about problems for environment and for public health due to chemical pesticides, the search for molecules more safe is currently of great importance. particularly, plants are able to fight the pathogens as insects, bacteria or fungi; so that plants could represent a valuable source of new molecules.201222536832
overexpression of mtcas31 enhances drought tolerance in transgenic arabidopsis by reducing stomatal density.• dehydrins are a type of late embryogenesis abundant protein. some dehydrins are involved in the response to various abiotic stresses. accumulation of dehydrins enhances the drought, cold and salt tolerances of transgenic plants, although the underlying mechanism is unclear. mtcas31 (medicago truncatula cold-acclimation specific protein 31) is a y(2)k(4)-type dehydrin that was isolated from medicago truncatula. • we analyzed the subcellular and histochemical localization of mtcas31, and the exp ...201222510066
ros production during symbiotic infection suppresses pathogenesis-related gene expression.leguminous plants have exclusive ability to form symbiotic relationship with soil bacteria of the genus rhizobium. symbiosis is a complex process that involves multiple molecular signaling activities, such as calcium fluxes, production of reactive oxygen species (ros) and synthesis of nodulation genes. we analyzed the role of ros in defense gene expression in medicago truncatula during symbiosis and pathogenesis. studies in arabidopsis thaliana showed that the induction of pathogenesis-related ( ...201222499208
how to hierarchize the main physiological processes responsible for phenotypic differences in large-scale screening studies?one difficulty when analyzing the determinants at the origin of plant phenotypic differences is that measured plant traits are frequently integrative: they result from the integration of a large number of physiological processes under the control of genetic and environmental factors. in a previous report, we demonstrated that dissecting integrative traits into simpler components using a simple crop physiology model was a valuable method for detecting quantitative trait loci (qtl) related to the ...201222499204
aeschynomene evenia, a model plant for studying the molecular genetics of the nod-independent rhizobium-legume symbiosis.research on the nitrogen-fixing symbiosis has been focused, thus far, on two model legumes, medicago truncatula and lotus japonicus, which use a sophisticated infection process involving infection thread formation. however, in 25% of the legumes, the bacterial entry occurs more simply in an intercellular fashion. among them, some aeschynomene spp. are nodulated by photosynthetic bradyrhizobium spp. that do not produce nod factors. this interaction is believed to represent a living testimony of t ...201222475377
texture of cellulose microfibrils of root hair cell walls of arabidopsis thaliana, medicago truncatula, and vicia sativa.cellulose is the most abundant biopolymer on earth, and has qualities that make it suitable for biofuel. there are new tools for the visualisation of the cellulose synthase complexes in living cells, but those do not show their product, the cellulose microfibrils (cmfs). in this study we report the characteristics of cell wall textures, i.e. the architectures of the cmfs in the wall, of root hairs of arabidopsis thaliana, medicago truncatula and vicia sativa and compare the different techniques ...201222458271
identification of distinct quantitative trait loci associated with defence against the closely related aphids acyrthosiphon pisum and a. kondoi in medicago truncatula.aphids are a major family of plant insect pests. medicago truncatula and acyrthosiphon pisum (pea aphid, pa) are model species with a suite of resources available to help dissect the mechanism underlying plant-aphid interactions. a previous study focused on monogenic and relatively strong resistance in m. truncatula to pa and other aphid species. in this study a moderate resistance to pa was characterized in detail in the m. truncatula line a17 and compared with the highly susceptible line a20 a ...201222442407
the small gtpase rop6 interacts with nfr5 and is involved in nodule formation in lotus japonicus.nod factor receptor5 (nfr5) is an atypical receptor-like kinase, having no activation loop in the protein kinase domain. it forms a heterodimer with nfr1 and is required for the early plant responses to rhizobium infection. a rho-like small gtpase from lotus japonicus was identified as an nfr5-interacting protein. the amino acid sequence of this rho-like gtpase is closest to the arabidopsis (arabidopsis thaliana) rop6 and medicago truncatula rop6 and was designated as ljrop6. the interaction bet ...201222434040
transcriptome sequencing of field pea and faba bean for discovery and validation of ssr genetic markers.field pea (pisum sativum l.) and faba bean (vicia faba l.) are cool-season grain legume species that provide rich sources of food for humans and fodder for livestock. to date, both species have been relative 'genomic orphans' due to limited availability of genetic and genomic information. a significant enrichment of genomic resources is consequently required in order to understand the genetic architecture of important agronomic traits, and to support germplasm enhancement, genetic diversity, pop ...201222433453
characterization of an isoflavonoid-specific prenyltransferase from lupinus albus.prenylated flavonoids and isoflavonoids possess antimicrobial activity against fungal pathogens of plants. however, only a few plant flavonoid and isoflavonoid prenyltransferase genes have been identified to date. in this study, an isoflavonoid prenyltransferase gene, designated as lapt1, was identified from white lupin (lupinus albus). the deduced protein sequence of lapt1 shared high homologies with known flavonoid and isoflavonoid prenyltransferases. the lapt1 gene was mainly expressed in roo ...201222430842
multiple domains in mtenod8 protein including the signal peptide target it to the symbiosome.symbiotic nitrogen fixation occurs in nodules, specialized organs on the roots of legumes. within nodules, host plant cells are infected with rhizobia that are encapsulated by a plant-derived membrane forming a novel organelle, the symbiosome. in medicago truncatula, the symbiosome consists of the symbiosome membrane, a single rhizobium, and the soluble space between them, called the symbiosome space. the symbiosome space is enriched with plant-derived proteins, including the m. truncatula early ...201222415512
genome-enabled insights into legume biology.legumes are the third-largest family of angiosperms, the second-most-important crop family, and a key source of biological nitrogen in agriculture. recently, the genome sequences of glycine max (soybean), medicago truncatula, and lotus japonicus were substantially completed. comparisons among legume genomes reveal a key role for duplication, especially a whole-genome duplication event approximately 58 mya that is shared by most agriculturally important legumes. a second and more recent genome du ...201222404476
the autoregulation gene sunn mediates changes in root organ formation in response to nitrogen through alteration of shoot-to-root auxin transport.we tested whether a gene regulating nodule number in medicago truncatula, super numeric nodules (sunn ), is involved in root architecture responses to carbon (c) and nitrogen (n) and whether this is mediated by changes in shoot-to-root auxin transport. nodules and lateral roots are root organs that are under the control of nutrient supply, but how their architecture is regulated in response to nutrients is unclear. we treated wild-type and sunn-1 seedlings with four combinations of low or increa ...201222399647
silencing of the rac1 gtpase mtrop9 in medicago truncatula stimulates early mycorrhizal and oomycete root colonizations but negatively affects rhizobial infection.rac/rop proteins (ρ-related gtpases of plants) are plant-specific small g proteins that function as molecular switches within elementary signal transduction pathways, including the regulation of reactive oxygen species (ros) generation during early microbial infection via the activation of nadph oxidase homologs of plants termed rboh (for respiratory burst oxidase homolog). we investigated the role of medicago truncatula jemalong a17 small gtpase mtrop9, orthologous to medicago sativa rac1, via ...201222399646
systems biology-based approaches toward understanding drought tolerance in food crops.economically important crops, such as maize, wheat, rice, barley, and other food crops are affected by even small changes in water potential at important growth stages. developing a comprehensive understanding of host response to drought requires a global view of the complex mechanisms involved. research on drought tolerance has generally been conducted using discipline-specific approaches. however, plant stress response is complex and interlinked to a point where discipline-specific approaches ...201322364373
polar localization of a symbiosis-specific phosphate transporter is mediated by a transient reorientation of secretion.the arbuscular mycorrhizal (am) symbiosis, formed by land plants and am fungi, evolved an estimated 400 million years ago and has been maintained in angiosperms, gymnosperms, pteridophytes, and some bryophytes as a strategy for enhancing phosphate acquisition. during am symbiosis, the am fungus colonizes the root cortical cells where it forms branched hyphae called arbuscules that function in nutrient exchange with the plant. each arbuscule is enveloped in a plant membrane, the periarbuscular me ...201222355114
role of cysteine residues and disulfide bonds in the activity of a legume root nodule-specific, cysteine-rich peptide.the root nodules of certain legumes including medicago truncatula produce >300 different nodule-specific cysteine-rich (ncr) peptides. medicago ncr antimicrobial peptides (amps) mediate the differentiation of the bacterium, sinorhizobium meliloti into a nitrogen-fixing bacteroid within the legume root nodules. in vitro, ncr amps such as ncr247 induced bacteroid features and exhibited antimicrobial activity against s. meliloti. the bacterial baca protein is critical to prevent s. meliloti from be ...201222351783
role of n-glycosylation sites and cxc motifs in trafficking of medicago truncatula nod factor perception protein to plasma membrane.the lysin motif receptor-like kinase, nfp (nod factor perception), is a key protein in the legume medicago truncatula for the perception of lipochitooligosaccharidic nod factors, which are secreted bacterial signals essential for establishing the nitrogen-fixing legume-rhizobia symbiosis. predicted structural and genetic analyses strongly suggest that nfp is at least part of a nod factor receptor, but few data are available about this protein. characterization of a variant encoded by the mutant ...201222334694
repeated leaf wounding alters the colonization of medicago truncatula roots by beneficial and pathogenic microorganisms.in nature, plants are subject to various stresses that are often accompanied by wounding of the aboveground tissues. as wounding affects plants locally and systemically, we investigated the impact of leaf wounding on interactions of medicago truncatula with root-colonizing microorganisms, such as the arbuscular mycorrhizal (am) fungus glomus intraradices, the pathogenic oomycete aphanomyces euteiches and the nitrogen-fixing bacterium sinorhizobium meliloti. to obtain a long-lasting wound respons ...201222329418
mtpar myb transcription factor acts as an on switch for proanthocyanidin biosynthesis in medicago truncatula.mtpar (medicago truncatula proanthocyanidin regulator) is an myb family transcription factor that functions as a key regulator of proanthocyanidin (pa) biosynthesis in the model legume medicago truncatula. mtpar expression is confined to the seed coat, the site of pa accumulation. loss-of-function par mutants contained substantially less pa in the seed coat than the wild type, whereas levels of anthocyanin and other specialized metabolites were normal in the mutants. in contrast, massive accumul ...201222307644
loss of abaxial leaf epicuticular wax in medicago truncatula irg1/palm1 mutants results in reduced spore differentiation of anthracnose and nonhost rust pathogens.to identify genes that confer nonhost resistance to biotrophic fungal pathogens, we did a forward-genetics screen using medicago truncatula tnt1 retrotransposon insertion lines. from this screen, we identified an inhibitor of rust germ tube differentation1 (irg1) mutant that failed to promote preinfection structure differentiation of two rust pathogens, phakopsora pachyrhizi and puccinia emaculata, and one anthracnose pathogen, colletotrichum trifolii, on the abaxial leaf surface. cytological an ...201222294617
functional domain analysis of the remorin protein ljsymrem1 in lotus japonicus.in legumes rhizobial infection during root nodule symbiosis (rns) is controlled by a conserved set of receptor proteins and downstream components. mtsymrem1, a protein of the remorin family in medicago truncatula, was shown to interact with at least three receptor-like kinases (rlks) that are essential for rns. remorins are comprised of a conserved c-terminal domain and a variable n-terminal region that defines the six different remorin groups. while both n- and c-terminal regions of remorins be ...201222292047
boosting the globalization of plant proteomics through inppo: current developments and future prospects.the international plant proteomics organization (inppo) is a non-profit-organization consisting of people who are involved or interested in plant proteomics. inppo is constantly growing in volume and activity, which is mostly due to the realization among plant proteomics researchers worldwide for the need of such a global platform. their active participation resulted in the rapid growth within the first year of inppo's official launch in 2011 via its website (www.inppo.com) and publication of th ...201222290804
(homo)glutathione deficiency impairs root-knot nematode development in medicago truncatula.root-knot nematodes (rkn) are obligatory plant parasitic worms that establish and maintain an intimate relationship with their host plants. during a compatible interaction, rkn induce the redifferentiation of root cells into multinucleate and hypertrophied giant cells essential for nematode growth and reproduction. these metabolically active feeding cells constitute the exclusive source of nutrients for the nematode. detailed analysis of glutathione (gsh) and homoglutathione (hgsh) metabolism de ...201222241996
characterization and genetic analysis of an ein4-like sequence (caetr-1) located in qtl(ar1) implicated in ascochyta blight resistance in chickpea.two alleles of a chickpea (cicer arietinum l.) ethylene receptor-like sequence (caetr-1) were sequence-characterized using synteny analysis with genome sequences of medicago truncatula l. the full length of the sequence obtained in the accession flip84-92c resistant to ascochyta blight (caetr-1a) span 4,428 bp, including the polyadenylation signal in the 3'-untranslated region (utr), whereas it has a 730 bp deletion in the 3'-utr region in the susceptible accession pi359075 (caetr-1b). the deduc ...201222238063
wuschel-related homeobox5 gene expression and interaction of cle peptides with components of the systemic control add two pieces to the puzzle of autoregulation of nodulation.in legumes, the symbiotic nodules are formed as a result of dedifferentiation and reactivation of cortical root cells. a shoot-acting receptor complex, similar to the arabidopsis (arabidopsis thaliana) clavata1 (clv1)/clv2 receptor, regulating development of the shoot apical meristem, is involved in autoregulation of nodulation (aon), a mechanism that systemically controls nodule number. the targets of clv1/clv2 in the shoot apical meristem, the wuschel (wus)-related homeobox (wox) family transc ...201222232385
phoma medicaginis stimulates the induction of the octadecanoid and phenylpropanoid pathways in medicago truncatula.gene expression changes and metabolite abundances were measured during the interaction of medicago truncatula with the fungal necrotrophic pathogen phoma medicaginis in leaf tissue of susceptible and resistant accessions. over 330 genes were differentially expressed in plants infected with p. medicaginis relative to mock-inoculated plants at 12 h post-inoculation. of these, 191 were induced in either the resistant or the susceptible accession, with 143 genes repressed. expression changes were ob ...201222212347
the re-establishment of desiccation tolerance in germinated arabidopsis thaliana seeds and its associated transcriptome.the combination of robust physiological models with "omics" studies holds promise for the discovery of genes and pathways linked to how organisms deal with drying. here we used a transcriptomics approach in combination with an in vivo physiological model of re-establishment of desiccation tolerance (dt) in arabidopsis thaliana seeds. we show that the incubation of desiccation sensitive (ds) germinated arabidopsis seeds in a polyethylene glycol (peg) solution re-induces the mechanisms necessary f ...201122195004
Triterpene Functional Genomics in Licorice for Identification of CYP72A154 Involved in the Biosynthesis of Glycyrrhizin.Glycyrrhizin, a triterpenoid saponin derived from the underground parts of Glycyrrhiza plants (licorice), has several pharmacological activities and is also used worldwide as a natural sweetener. The biosynthesis of glycyrrhizin involves the initial cyclization of 2,3-oxidosqualene to the triterpene skeleton ß-amyrin, followed by a series of oxidative reactions at positions C-11 and C-30, and glycosyl transfers to the C-3 hydroxyl group. We previously reported the identification of a cytochrome ...201122128119
mining functional microsatellites in legume unigenes.highly polymorphic and transferable microsatellites (ssrs) are important for comparative genomics, genome analysis and phylogenetic studies. development of novel species-specific microsatellite markers remains a costly and labor-intensive project. therefore, interest has been shifted from genomic to genic markers owing to their high inter-species transferability as they are developed from conserved coding regions of the genome. this study concentrates on comparative analysis of genic microsatell ...201122125396
legumeip: an integrative database for comparative genomics and transcriptomics of model legumes.legumes play a vital role in maintaining the nitrogen cycle of the biosphere. they conduct symbiotic nitrogen fixation through endosymbiotic relationships with bacteria in root nodules. however, this and other characteristics of legumes, including mycorrhization, compound leaf development and profuse secondary metabolism, are absent in the typical model plant arabidopsis thaliana. we present legumeip (http://plantgrn.noble.org/legumeip/), an integrative database for comparative genomics and tran ...201222110036
the medicago genome provides insight into the evolution of rhizobial symbioses.legumes (fabaceae or leguminosae) are unique among cultivated plants for their ability to carry out endosymbiotic nitrogen fixation with rhizobial bacteria, a process that takes place in a specialized structure known as the nodule. legumes belong to one of the two main groups of eurosids, the fabidae, which includes most species capable of endosymbiotic nitrogen fixation. legumes comprise several evolutionary lineages derived from a common ancestor 60 million years ago (myr ago). papilionoids ar ...201122089132
contribution of nfp lysm domains to the recognition of nod factors during the medicago truncatula/sinorhizobium meliloti symbiosis.the root nodule nitrogen fixing symbiosis between legume plants and soil bacteria called rhizobia is of great agronomical and ecological interest since it provides the plant with fixed atmospheric nitrogen. the establishment of this symbiosis is mediated by the recognition by the host plant of lipo-chitooligosaccharides called nod factors (nfs), produced by the rhizobia. this recognition is highly specific, as precise nf structures are required depending on the host plant. here, we study the imp ...201122087221
Regulation of Compound Leaf Development in Medicago truncatula by Fused Compound Leaf1, a Class M KNOX Gene.Medicago truncatula is a legume species belonging to the inverted repeat lacking clade (IRLC) with trifoliolate compound leaves. However, the regulatory mechanisms underlying development of trifoliolate leaves in legumes remain largely unknown. Here, we report isolation and characterization of fused compound leaf1 (fcl1) mutants of M. truncatula. Phenotypic analysis suggests that FCL1 plays a positive role in boundary separation and proximal-distal axis development of compound leaves. Map-based ...201122080596
comparative analysis of mirnas and their targets across four plant species.microrna (mirna) mediated regulation of gene expression has been recognized as a major posttranscriptional regulatory mechanism also in plants. we performed a comparative analysis of mirnas and their respective gene targets across four plant species: arabidopsis thaliana (ath), medicago truncatula(mtr), brassica napus (bna), and chlamydomonas reinhardtii (cre).201122067256
lc/ms profiling of flavonoid glycoconjugates isolated from hairy roots, suspension root cell cultures and seedling roots of medicago truncatula.hairy roots and suspension cell cultures are commonly used in deciphering different problems related to the biochemistry and physiology of plant secondary metabolites. here, we address about the issue of possible differences in the profiles of flavonoid compounds and their glycoconjugates derived from various plant materials grown in a standard culture media. we compared profiles of flavonoids isolated from seedling roots, hairy roots, and suspension root cell cultures of a model legume plant, m ...201122039365
strigolactone biosynthesis in medicago truncatula and rice requires the symbiotic gras-type transcription factors nsp1 and nsp2.legume gras (gai, rga, scr)-type transcription factors nodulation signaling pathway1 (nsp1) and nsp2 are essential for rhizobium nod factor-induced nodulation. both proteins are considered to be nod factor response factors regulating gene expression after symbiotic signaling. however, legume nsp1 and nsp2 can be functionally replaced by nonlegume orthologs, including rice (oryza sativa) nsp1 and nsp2, indicating that both proteins are functionally conserved in higher plants. here, we show that n ...201122039214
CYP716A Subfamily Members are Multifunctional Oxidases in Triterpenoid Biosynthesis.Triterpenoids are a diverse group of secondary metabolites that are associated with a variety of biological activities. Oleanolic acid, ursolic acid and betulinic acid are common triterpenoids in plants with diverse biological activities, including antifungal, antibacterial, anti-human immunodeficiency virus (HIV) and/or antitumor activities. In the present study, using the gene co-expression analysis tool of Medicago truncatula, we found a strong correlation between CYP716A12 and ß-amyrin synth ...201122039103
laser microdissection unravels cell-type-specific transcription in arbuscular mycorrhizal roots, including caat-box transcription factor gene expression correlating with fungal contact and spread.arbuscular mycorrhizae (am) are the most widespread symbioses on earth, promoting nutrient supply of most terrestrial plant species. to unravel gene expression in defined stages of medicago truncatula root colonization by am fungi, we here combined genome-wide transcriptome profiling based on whole mycorrhizal roots with real-time reverse transcription-pcr experiments that relied on characteristic cell types obtained via laser microdissection. our genome-wide approach delivered a core set of 512 ...201122034628
A phylogenetic strategy based on a legume-specific whole genome duplication yields symbiotic cytokinin type-a response regulators.Legumes host their Rhizobium spp. symbiont in novel root organs called nodules. Nodules originate from differentiated root cortical cells that dedifferentiate and subsequently form nodule primordia, a process controlled by cytokinin. A whole-genome duplication has occurred at the root of the legume Papilionoideae subfamily. We hypothesize that gene pairs originating from this duplication event and are conserved in distinct Papilionoideae lineages have evolved symbiotic functions. A phylogenetic ...201122034625
protection of sinorhizobium against host cysteine-rich antimicrobial peptides is critical for symbiosis.sinorhizobium meliloti differentiates into persisting, nitrogen-fixing bacteroids within root nodules of the legume medicago truncatula. nodule-specific cysteine-rich antimicrobial peptides (ncr amps) and the bacterial baca protein are essential for bacteroid development. however, the bacterial factors central to the ncr amp response and the in planta role of baca are unknown. we investigated the hypothesis that baca is critical for the bacterial response towards ncr amps. we found that baca was ...201121990963
Analysis of the subcellular localisation of lipoxygenase in legume and actinorhizal nodules.Plant lipoxygenases (LOXs; EC 1.13.11.12) catalyse the oxygenation of polyunsaturated fatty acids, linoleic (18:2) and a-linolenic acid (18:3(n-3)) and are involved in processes such as stress responses and development. Depending on the regio-specificity of a LOX, the incorporation of molecular oxygen leads to formation of 9- or 13-fatty acid hydroperoxides, which are used by LOX itself as well as by members of at least six different enzyme families to form a series of biologically active molecu ...201221973171
Recent Progress in Development of Tnt1 Functional Genomics Platform for Medicago truncatula and Lotus japonicus in Bulgaria.Legumes, as protein-rich crops, are widely used for human food, animal feed and vegetable oil production. Over the past decade, two legume species, Medicago truncatula and Lotus japonicus, have been adopted as model legumes for genomics and physiological studies. The tobacco transposable element, Tnt1, is a powerful tool for insertional mutagenesis and gene inactivation in plants. A large collection of Tnt1-tagged lines of M. truncatula cv. Jemalong was generated during the course of the project ...201121966253
from model to crop: functional analysis of a stay-green gene in the model legume medicago truncatula and effective use of the gene for alfalfa improvement.medicago truncatula has been developed into a model legume. its close relative alfalfa (medicago sativa) is the most widely grown forage legume crop in the united states. by screening a large population of m. truncatula mutants tagged with the transposable element of tobacco (nicotiana tabacum) cell type1 (tnt1), we identified a mutant line (nf2089) that maintained green leaves and showed green anthers, central carpels, mature pods, and seeds during senescence. genetic and molecular analyses rev ...201121957014
whole-genome nucleotide diversity, recombination, and linkage disequilibrium in the model legume medicago truncatula.medicago truncatula is a model for investigating legume genetics, including the genetics and evolution of legume-rhizobia symbiosis. we used whole-genome sequence data to identify and characterize sequence polymorphisms and linkage disequilibrium (ld) in a diverse collection of 26 m. truncatula accessions. our analyses reveal that m. truncatula harbors both higher diversity and less ld than soybean (glycine max) and exhibits patterns of ld and recombination similar to arabidopsis thaliana. the p ...201121949378
proteomic analysis by two-dimensional differential in gel electrophoresis (2d dige) of the early response of pisum sativum to orobanche crenata.crenate broomrape (orobanche crenata) is considered to be the major constraint for legume crops in mediterranean countries. strategies of control have been developed, but only marginal successes have been achieved. for the efficient control of the parasite, a better understanding of its interaction and associated resistance mechanisms at the molecular level is required. the pea response to this parasitic plant and the molecular basis of the resistance was studied using a proteomic approach based ...201221920908
glutamine synthetase is a molecular target of nitric oxide in root nodules of medicago truncatula and is regulated by tyrosine nitration.nitric oxide (no) is emerging as an important regulatory player in the rhizobium-legume symbiosis, but its biological role in nodule functioning is still far from being understood. to unravel the signal transduction cascade and ultimately no function, it is necessary to identify its molecular targets. this study provides evidence that glutamine synthetase (gs), a key enzyme for root nodule metabolism, is a molecular target of no in root nodules of medicago truncatula, being regulated by tyrosine ...201121914816
standardizing seed dormancy research.seeds are very attractive and convenient for molecular genetic studies that challenge principal biological phenomena related to the initiation and suppression of growth (e.g., germination and dormancy, respectively). the number of reports in this field is rapidly expanding. seed dormancy is a widely misinterpreted biological attribute. one of the main reasons is the general neglect of reliable dormancy assays; often, the sole criterion of current dormancy assays is the total germination of a see ...201121898248
the mitochondrial proteome of the model legume medicago truncatula.legumes carry out special biochemical functions, e.g. the fixation of molecular nitrogen based on a symbiosis with proteobacteria. at the cellular level, this symbiosis has to be implemented into the energy metabolism of the host cell. to provide a basis for future analyses, we have characterized the protein complement of mitochondria of the model legume medicago truncatula using two-dimensional isoelectric focussing (ief) and blue-native (bn)-sds-page. while the ief reference map resulted mainl ...201121893218
the rice miniature inverted repeat transposable element mping is an effective insertional mutagen in soybean.insertional mutagenesis of legume genomes such as soybean (glycine max) should aid in identifying genes responsible for key traits such as nitrogen fixation and seed quality. the relatively low throughput of soybean transformation necessitates the use of a transposon-tagging strategy where a single transformation event will produce many mutations over a number of generations. however, existing transposon-tagging tools being used in legumes are of limited utility because of restricted transpositi ...201121844309
[cloning and characterization of 3-hydroxy-3-methylglutary coa reductase cdna of glycyrrhiza uralensis].to clone and analysis the sequence of 3-hydroxy-3-methylglutary coa reductase (hmgr) cdna from glycyrrhiza uralensis.201121837964
medicago truncatula cyp716a12 is a multifunctional oxidase involved in the biosynthesis of hemolytic saponins.saponins, a group of glycosidic compounds present in several plant species, have aglycone moieties that are formed using triterpenoid or steroidal skeletons. in spite of their importance as antimicrobial compounds and their possible benefits for human health, knowledge of the genetic control of saponin biosynthesis is still poorly understood. in the medicago genus, the hemolytic activity of saponins is related to the nature of their aglycone moieties. we have identified a cytochrome p450 gene (c ...201121821776
pseudonodule formation by wild type and symbiotic mutant medicago truncatula in response to auxin transport inhibitors.rhizobium and allied bacteria form symbiotic nitrogen fixing nodules on legume roots. plant hormones play key roles in nodule formation. we treated medicago truncatula roots with auxin transport inhibitors (atis) n-(1-naphthyl)phthalamic acid (npa) and 2,3,5-triiodobenzoic acid (tiba), to induce the formation of pseudonodules. m. truncatula mutants defective for rhizobial nod factor signal transduction still formed pseudonodules in response to atis. however, a m. truncatula ethylene-insensitive ...201121809981
molecular adaptation in flowering and symbiotic recognition pathways: insights from patterns of polymorphism in the legume medicago truncatula.abstract: background: we studied patterns of molecular adaptation in the wild mediterranean legume medicago truncatula. we focused on two phenotypic traits that are not functionally linked: flowering time and perception of symbiotic microbes. phenology is an important fitness component, especially for annual plants, and many instances of molecular adaptation have been reported for genes involved in flowering pathways. while perception of symbiotic microbes is also integral to adaptation in many ...201121806823
capturing cold-stress-related sequence diversity from a wild relative of common bean (phaseolus angustissimus).one restriction to the cultivation of common bean, phaseolus vulgaris l., is its limited tolerance to low temperatures. in the present study, subtraction suppression hybridization was employed to enrich for stress responsive genes in both a chilling-susceptible common bean and a relatively more chilling-tolerant wild bean species, phaseolus angustissimus. for each species, approximately 11 000 expressed sequence tags were generated. comparative sequence analysis of the est collection with the av ...201121797793
expression analysis of clavata1-like and nodulin21-like genes from pinus sylvestris during ectomycorrhiza formation.the ecology and physiology of ectomycorrhizal (ecm) symbiosis with conifer trees are well documented. in comparison, however, very little is known about the molecular regulation of these associations. in an earlier study, we identified three ecm-regulated pinus expressed sequence tags (est), two of which were identified as homologous to the medicago truncatula nodulin mtn21. the third est was a homologue to the receptor-like kinase clavata1. we have characterized the expression patterns of these ...201121751039
the exudate from an arbuscular mycorrhizal fungus induces nitric oxide accumulation in medicago truncatula roots.nitric oxide (no) is a signaling molecule involved in plant responses to abiotic and biotic stresses. while there is evidence for no accumulation during legume nodulation, almost no information exists for arbuscular mycorrhizas (am). here, we investigated the occurrence of no in the early stages of medicago truncatula-gigaspora margarita interaction, focusing on the plant response to fungal diffusible molecules. no was visualized in root organ cultures and seedlings by confocal microscopy using ...201121744141
symbiotic rhizobia bacteria trigger a change in localization and dynamics of the medicago truncatula receptor kinase lyk3.to form nitrogen-fixing symbioses, legume plants recognize a bacterial signal, nod factor (nf). the legume medicago truncatula has two predicted nf receptors that direct separate downstream responses to its symbiont sinorhizobium meliloti. nod factor perception encodes a putative low-stringency receptor that is responsible for calcium spiking and transcriptional responses. lysin motif receptor-like kinase3 (lyk3) encodes a putative high-stringency receptor that mediates bacterial infection. we l ...201121742993
the root determined nodulation1 gene regulates nodule number in roots of medicago truncatula and defines a highly conserved, uncharacterized plant gene family.the formation of nitrogen-fixing nodules in legumes is tightly controlled by a long-distance signaling system in which nodulating roots signal to shoot tissues to suppress further nodulation. a screen for supernodulating medicago truncatula mutants defective in this regulatory behavior yielded loss-of-function alleles of a gene designated root determined nodulation1 (rdn1). grafting experiments demonstrated that rdn1 regulatory function occurs in the roots, not the shoots, and is essential for n ...201121742814
durable broad-spectrum powdery mildew resistance in pea er1 plants is conferred by natural loss-of-function mutations in psmlo1.loss-of-function alleles of plant-specific mlo (mildew resistance locus o) genes confer broad-spectrum powdery mildew resistance in monocot (barley) and dicot (arabidopsis thaliana, tomato) plants. recessively inherited powdery mildew resistance in pea (pisum sativum) er1 plants is, in many aspects, reminiscent of mlo-conditioned powdery mildew immunity, yet the underlying gene has remained elusive to date. we used a polymerase chain reaction (pcr)-based approach to amplify a candidate mlo cdna ...201121726385
expression of coordinately regulated defence response genes and analysis of their role in disease resistance in medicago truncatula.microarray technology was used to identify the genes associated with disease defence responses in the model legume medicago truncatula. transcript profiles from m. truncatula cv. jemalong genotype a17 leaves inoculated with colletotrichum trifolii and erysiphe pisi and roots infected with phytophthora medicaginis were compared to identify the genes expressed in response to all three pathogens and genes unique to an interaction. the a17 genotype is resistant to c. trifolii and e. pisi, exhibiting ...201121726379
STENOFOLIA Regulates Blade Outgrowth and Leaf Vascular Patterning in Medicago truncatula and Nicotiana sylvestris.Dicot leaf primordia initiate at the flanks of the shoot apical meristem and extend laterally by cell division and cell expansion to form the flat lamina, but the molecular mechanism of lamina outgrowth remains unclear. Here, we report the identification of STENOFOLIA (STF), a WUSCHEL-like homeobox transcriptional regulator, in Medicago truncatula, which is required for blade outgrowth and leaf vascular patterning. STF belongs to the MAEWEST clade and its inactivation by the transposable element ...201121719692
transcriptional profiling of medicago truncatula under salt stress identified a novel cbf transcription factor mtcbf4 that plays an important role in abiotic stress responses.salt stress hinders the growth of plants and reduces crop production worldwide. however, different plant species might possess different adaptive mechanisms to mitigate salt stress. we conducted a detailed pathway analysis of transcriptional dynamics in the roots of medicago truncatula seedlings under salt stress and selected a transcription factor gene, mtcbf4, for experimental validation.201121718548
developmental analysis of a medicago truncatula smooth leaf margin1 mutant reveals context-dependent effects on compound leaf development.compound leaf development requires highly regulated cell proliferation, differentiation, and expansion patterns. we identified loss-of-function alleles at the smooth leaf margin1 (slm1) locus in medicago truncatula, a model legume species with trifoliate adult leaves. slm1 encodes an auxin efflux carrier protein and is the ortholog of arabidopsis thaliana pin-formed1 (pin1). auxin distribution is impaired in the slm1 mutant, resulting in pleiotropic phenotypes in different organs. the most strik ...201121693694
medicago truncatula ipd3 is a member of the common symbiotic signaling pathway required for rhizobial and mycorrhizal symbioses.legumes form endosymbiotic associations with nitrogen fixing bacteria and arbuscular mycorrhizal (am) fungi which facilitate nutrient uptake. both symbiotic interactions require a molecular signal exchange between the plant and the symbiont and this involves a conserved symbiosis (sym) signaling pathway. in order to identify plant genes required for intracellular accommodation of nitrogen-fixing bacteria and am fungi, we characterized medicago truncatula symbiotic mutants defective for rhizobial ...201121692638
the medicago flowering locus t homolog, mtfta1, is a key regulator of flowering time.flowering locus t (ft) genes encode proteins that function as the mobile floral signal, florigen. in this study, we characterized five ft-like genes from the model legume, medicago (medicago truncatula). the different ft genes showed distinct patterns of expression and responses to environmental cues. three of the ft genes (mtfta1, mtftb1, and mtftc) were able to complement the arabidopsis (arabidopsis thaliana) ft-1 mutant, suggesting that they are capable of functioning as florigen. mtfta1 is ...201121685176
mtbhlh1, a bhlh transcription factor involved in medicago truncatula nodule vascular patterning and nodule to plant metabolic exchanges.this study aimed at defining the role of a basic helix-loop-helix (bhlh) transcription factor gene from medicago truncatula, mtbhlh1, whose expression is upregulated during the development of root nodules produced upon infection by rhizobia bacteria. we used mtbhlh1 promoter::gus fusions and quantitative reverse-transcription polymerase chain reaction analyses to finely characterize the mtbhlh1 expression pattern. we altered mtbhlh1 function by expressing a dominantly repressed construct (cres-t ...201121679315
harvest-inducibility of the promoter of alfalfa s-adenosyl-l: -methionine: trans-caffeoyl-coa3-o-methyltransferase gene.a major limitation on the expression of some foreign proteins in transgenic plants is the toxic effect of such proteins on the host plant resulting in inhibition of normal growth and development. a solution to this problem is to control the expression of genes for such proteins by means of inducible promoters, as is frequently done in microbial systems. a cdna clone was obtained from subtractive hybridization of non-harvested and harvested alfalfa leaf tissue, named hi12. the hi12 cdna was ident ...201121667113
border sequences of medicago truncatula cle36 are specifically cleaved by endoproteases common to the extracellular fluids of medicago and soybean.cle (clavata3/esr-related) peptides are developmental regulators that are secreted into the apoplast. little is known about the role of the sequences that flank cle peptides in terms of their biological activity or how they are targeted by proteases that are known to liberate the final active cle peptides from their precursor sequences. the biological activity of medicago truncatula cle36, which possesses broadly conserved border sequences flanking the putative final active cle36 peptide product ...201121633083
large-scale transcriptome analysis in chickpea (cicer arietinum l.), an orphan legume crop of the semi-arid tropics of asia and africa.chickpea (cicer arietinum l.) is an important legume crop in the semi-arid regions of asia and africa. gains in crop productivity have been low however, particularly because of biotic and abiotic stresses. to help enhance crop productivity using molecular breeding techniques, next generation sequencing technologies such as roche/454 and illumina/solexa were used to determine the sequence of most gene transcripts and to identify drought-responsive genes and gene-based molecular markers. a total o ...201121615673
transcriptome sequencing of lentil based on second-generation technology permits large-scale unigene assembly and ssr marker discovery.abstract: background: lentil (lens culinaris medik.) is a cool-season grain legume which provides a rich source of protein for human consumption. in terms of genomic resources, lentil is relatively underdeveloped, in comparison to other fabaceae species, with limited available data. there is hence a significant need to enhance such resources in order to identify novel genes and alleles for molecular breeding to increase crop productivity and quality. results: tissue-specific cdna samples from si ...201121609489
transformed hairy roots of discaria trinervis: a valuable tool for studying actinorhizal symbiosis in the context of intercellular infection.among infection mechanisms leading to root nodule symbiosis, the intercellular infection pathway is probably the most ancestral but also one of the least characterized. intercellular infection has been described in discaria trinervis, an actinorhizal plant belonging to the rosales order. to decipher the molecular mechanisms underlying intercellular infection with frankia, we set up an efficient genetic transformation protocol for d. trinervis based on a. rhizogenes. we showed that composite plan ...201121585269
stars and symbiosis: microrna- and microrna*-mediated transcript cleavage involved in arbuscular mycorrhizal symbiosis.the majority of plants are able to form the arbuscular mycorrhizal (am) symbiosis in association with am fungi. during symbiosis development, plant cells undergo a complex reprogramming resulting in profound morphological and physiological changes. micrornas (mirnas) are important components of the regulatory network of plant cells. to unravel the impact of mirnas and mirna-mediated mrna cleavage on root cell reprogramming during am symbiosis, we carried out high-throughput (illumina) sequencing ...201121571671
in medicago truncatula, water deficit modulates the transcript accumulation of components of small rna pathways.small rnas (srnas) are 20-24 nucleotide (nt) rnas and are involved in plant development and response to abiotic stresses. plants have several srna pathways implicated in the transcriptional and post-transcriptional silencing of gene expression. two key enzyme families common to all pathways are the dicer-like (dcl) proteins involved in srnas maturation and the argonautes (agos) involved in the targeting and functional action of srnas. post-transcriptional silencing mediated by agos may occur by ...201121569262
crosstalk between the nodulation signaling pathway and the autoregulation of nodulation in medicago truncatula.• a subset of clavata3/endosperm-surrounding region-related (cle) peptides are involved in autoregulation of nodulation (aon) in medicago truncatula (e.g. mtcle12 and mtcle13). however, their linkage to other components of the aon pathways downstream of the shoot-derived inhibitor (sdi) is not understood. • we have ectopically expressed the putative peptide ligand encoding genes mtcle12 and mtcle13 in m. truncatula which abolished nodulation completely in wild-type roots but not in the supernodu ...201121507004
generation of composite plants in medicago truncatula used for nodulation assays.similar to agrobacterium tumerfaciens, agrobacterium rhizogenes can transfer foreign dnas into plant cells based on the autonomous root-inducing (ri) plasmid. a. rhizogenes can cause hairy root formation on plant tissues and form composite plants after transformation. on these composite plants, some of the regenerated roots are transgenic, carrying the wild type t-dna and the engineered binary vector; while the shoots are still non-transgenic, serving to provide energy and growth support. these ...201121490571
developing a method for customized induction of flowering.the ability to induce flowering on demand is of significant biotechnological interest. ft protein has been recently identified as an important component of the mobile flowering hormone, florigen, whose function is conserved across the plant kingdom. we therefore focused on manipulation of both endogenous and heterologous ft genes to develop a floral induction system where flowering would be inhibited until it was induced on demand. the concept was tested in the model plant arabidopsis thaliana ( ...201121481273
symbiont identity matters: carbon and phosphorus fluxes between medicago truncatula and different arbuscular mycorrhizal fungi.many studies have scrutinized the nutritional benefits of arbuscular mycorrhizal associations to their host plants, while the carbon (c) balance of the symbiosis has often been neglected. here, we present quantification of both the c costs and the phosphorus (p) uptake benefits of mycorrhizal association between barrel medic (medicago truncatula) and three arbuscular mycorrhizal fungal species, namely glomus intraradices, glomus claroideum, and gigaspora margarita. plant growth, p uptake and c a ...201121472448
mate2 mediates vacuolar sequestration of flavonoid glycosides and glycoside malonates in medicago truncatula.the majority of flavonoids, such as anthocyanins, proanthocyanidins, and isoflavones, are stored in the central vacuole, but the molecular basis of flavonoid transport is still poorly understood. here, we report the functional characterization of a multidrug and toxin extrusion transporter (mate2), from medicago truncatula. mate 2 is expressed primarily in leaves and flowers. despite its high similarity to the epicatechin 3'-o-glucoside transporter mate1, mate2 cannot efficiently transport proan ...201121467581
identification and characterisation of seed storage protein transcripts from lupinus angustifolius.in legumes, seed storage proteins are important for the developing seedling and are an important source of protein for humans and animals. lupinus angustifolius (l.), also known as narrow-leaf lupin (nll) is a grain legume crop that is gaining recognition as a potential human health food as the grain is high in protein and dietary fibre, gluten-free and low in fat and starch.201121457583
nitric oxide is required for an optimal establishment of the medicago truncatula-sinorhizobium meliloti symbiosis.nitric oxide (no) is a gaseous molecule that participates in numerous plant signalling pathways. it is involved in plant responses to pathogens and development processes such as seed germination, flowering and stomatal closure. using a permeable no-specific fluorescent probe and a bacterial reporter strain expressing the lacz gene under the control of a no-responsive promoter, we detected no production in the first steps, during infection threads growth, of the medicago truncatula-sinorhizobium ...201121457261
leveraging genomic resources of model species for the assessment of diversity and phylogeny in wild and domesticated lentil.advances in comparative genomics have provided significant opportunities for analysis of genetic diversity in species with limited genomic resources, such as the genus lens. medicago truncatula expressed sequence tags (ests) were aligned with the arabidopsis thaliana genome sequence to identify conserved exon sequences and splice sites in the ests. conserved primers (cps) based on m. truncatula est sequences flanking one or more introns were then designed. a total of 22% of the cps produced poly ...201121454287
evolution of the number of lrrs in plant disease resistance genes.the largest group of plant resistance (r) genes contain the regions that encode the nucleotide-binding site (nbs) and leucine-rich repeat (lrr) domains (nbs-lrr genes). to gain new resistance, amino acid substitutions and changes in number of the lrrs that recognize the presence of pathogens are considered important. in this study, we focus on the evolution of the number of lrrs and analyze the genome data of five plant species, arabidopsis thaliana, oryza sativa, medicago truncatula, lotus japo ...201121442326
plantmirnapred: efficient classification of real and pseudo plant pre-mirnas.micrornas (mirnas) are a set of short (21-24 nt) non-coding rnas that play significant roles as post-transcriptional regulators in animals and plants. while some existing methods use comparative genomic approaches to identify plant precursor mirnas (pre-mirnas), others are based on the complementarity characteristics between mirnas and their target mrnas sequences. however, they can only identify the homologous mirnas or the limited complementary mirnas. furthermore, since the plant pre-mirnas a ...201121441575
interactions of arabidopsis and m. truncatula with the same pathogens differ in dependence on ethylene and ethylene response factors.microbial pathogens inflict large losses to agriculture annually and thus mechanisms of plant resistance and how to deploy them to enhance disease resistance in crops are foci of much research interest. we recently described the important role of ethylene and ethylene response transcription factors (erfs), particularly mterf1-1, in mediating resistance to the fungal pathogen rhizoctonia solani in the model legume, medicago truncatula. previous studies on the closely related aterf14, a master reg ...201121389781
characterisation of the legume serk-nik gene superfamily including splice variants: implications for development and defence.somatic embryogenesis receptor-like kinase (serk) genes are part of the regulation of diverse signalling events in plants. current evidence shows serk proteins function both in developmental and defence signalling pathways, which occur in response to both peptide and steroid ligands. serks are generally present as small gene families in plants, with five serk genes in arabidopsis. knowledge gained primarily through work on arabidopsis serks indicates that these proteins probably interact with a ...201121385462
development and use of genic molecular markers (gmms) for construction of a transcript map of chickpea (cicer arietinum l.).a transcript map has been constructed by the development and integration of genic molecular markers (gmms) including single nucleotide polymorphism (snp), genic microsatellite or simple sequence repeat (ssr) and intron spanning region (isr)-based markers, on an inter-specific mapping population of chickpea, the third food legume crop of the world and the first food legume crop of india. for snp discovery through allele re-sequencing, primer pairs were designed for 688 genes/expressed sequence ta ...201121384113
a legume specific protein database (legprot) improves the number of identified peptides, confidence scores and overall protein identification success rates for legume proteomics.a legume specific protein database (legprot) has been created containing sequences from seven legume species, i.e., glycine max, lotus japonicus, medicago sativa, medicago truncatula, lupinusalbus, phaseolus vulgaris, and pisum sativum. the database consists of amino acid sequences translated from predicted gene models and 6-frame translations of tentative consensus (tc) sequences assembled from expressed sequence tags (ests) and singleton ests. this database was queried using mass spectral data ...201121353266
phosphorous deficiency decreases nitrogenase activity but increases proton efflux in n2-fixing medicago truncatula.effects of sinorhizobium strain and p nutrition on n(2)-dependent growth, nitrogenase activity and proton efflux by nodulated roots were investigated in the model legume medicago truncatula cultivar jemalong grown in hydroaeroponic culture in symbioses with sinorhizobium meliloti strains 102f51 and 2011. sinorhizobium strain had strong effects on nitrogenase activity and n(2)-dependent growth, with s. meliloti 102f51 being the more efficient strain. apparent and total nitrogenase activities, mea ...201121334214
genetic dissection of rhizobium-induced infection and nodule organogenesis in pea based on enod12a and enod5 expression analysis.in legumes, perception of rhizobial lipochitooligosacharide-based molecules (nod factors) and subsequent signal transduction triggers transcription of plant symbiosis-specific genes (early nodulins). we present genetic dissection of nod factor-controlled processes in pisum sativum using two early nodulin genes psenod12a and psenod5, that are differentially up-regulated during symbiosis. a novel set of non-nodulating pea mutants in fourteen loci was examined, among which seven loci are not descri ...201021309975
transcription reprogramming during root nodule development in medicago truncatula.many genes which are associated with root nodule development and activity in the model legume medicago truncatula have been described. however information on precise stages of activation of these genes and their corresponding transcriptional regulators is often lacking. whether these regulators are shared with other plant developmental programs also remains an open question. here detailed microarray analyses have been used to study the transcriptome of root nodules induced by either wild type or ...201121304580
a stress-associated protein containing a20/an1 zing-finger domains expressed in medicago truncatula seeds.mtsap1 (medicago truncatula stress-associated protein 1) was revealed as a down-regulated gene by suppressive subtractive hybridization between two mrna populations of embryo axes harvested before and after radicle emergence. mtsap1 is the first gene encoding a sap with a20 and an1 zinc-finger domains characterized in m. truncatula. mtsap1 protein shares 54% and 62% homology with atsap7 (arabidopsis thaliana) and osisap8 (oryza sativa) respectively, with in particular a strong homology in the a2 ...201121296585
comparative evolution of photosynthetic genes in response to polyploid and nonpolyploid duplication.the likelihood of duplicate gene retention following polyploidy varies by functional properties (e.g. gene ontologies or protein family domains), but little is known about the effects of whole-genome duplication on gene networks related by a common physiological process. here, we examined the effects of both polyploid and nonpolyploid duplications on genes encoding the major functional groups of photosynthesis (photosystem i, photosystem ii, the light-harvesting complex, and the calvin cycle) in ...201121289102
the pea gigas gene is a flowering locus t homolog necessary for graft-transmissible specification of flowering but not for responsiveness to photoperiod.garden pea (pisum sativum) was prominent in early studies investigating the genetic control of flowering and the role of mobile flowering signals. in view of recent evidence that genes in the flowering locus t (ft) family play an important role in generating mobile flowering signals, we isolated the ft gene family in pea and examined the regulation and function of its members. comparison with medicago truncatula and soybean (glycine max) provides evidence of three ancient subclades (fta, ftb, an ...201121282524
identification and characterization of thioredoxin h isoforms differentially expressed in germinating seeds of the model legume medicago truncatula.thioredoxins (trxs) h, small disulfide reductases, and nadp-thioredoxin reductases (ntrs) have been shown to accumulate in seeds of different plant species and play important roles in seed physiology. however, little is known about the identity, properties, and subcellular location of trx h isoforms that are abundant in legume seeds. to fill this gap, in this work, we characterized the trx h family of medicago truncatula, a model legume, and then explored the activity and localization of trx h i ...201121239621
vapyrin, a gene essential for intracellular progression of arbuscular mycorrhizal symbiosis, is also essential for infection by rhizobia in the nodule symbiosis of medicago truncatula.intracellular invasion of root cells is required for the establishment of successful endosymbioses in legumes of both arbuscular mycorrhizal (am) fungi and rhizobial bacteria. in both interactions a requirement for successful entry is the activation of a common signalling pathway that includes five genes required to generate calcium oscillations and two genes required for the perception of the calcium response. recently, it has been discovered that in medicago truncatula, the vapyrin (vpy) gene ...201021223389
Refresh
firstprev
12345678910...
nextlast
Displaying items 401 - 500 of 1111