Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| secret agent, an arabidopsis thaliana o-glcnac transferase, modifies the plum pox virus capsid protein. | the capsid protein of plum pox virus (ppv-cp) is modified with o-linked glcnac (o-glcnac). while arabidopsis has two o-glcnac transferases, secret agent (sec) and spindly (spy), previous work suggests that sec modifies ppv-cp and that the modification plays a role in the infection process. here, we show that when co-expressed in escherichia coli sec modifies ppv-cp. deletion mapping and site-directed mutagenesis identified three threonine and a serine located near the n-terminus of ppv-cp that a ... | 2006 | 17027982 |
| salicylic acid-mediated and rna-silencing defense mechanisms cooperate in the restriction of systemic spread of plum pox virus in tobacco. | plum pox virus (ppv) is able to replicate in inoculated leaves of nicotiana tabacum, but is defective in systemic movement in this host. however, ppv produces a systemic infection in transgenic tobacco expressing the silencing suppressor p1/hc-pro from tobacco etch virus (tev). in this work we show that ppv is able to move to upper non-inoculated leaves of tobacco plants expressing bacterial salicylate hydroxylase (nahg) that degrades salicylic acid (sa). replication and accumulation of ppv is h ... | 2006 | 17018032 |
| mapping of two o-glcnac modification sites in the capsid protein of the potyvirus plum pox virus. | a large number of o-linked n-acetylglucosamine (o-glcnac) residues have been mapped in vertebrate proteins, however targets of o-glcnacylation in plants still have not been characterized. we show here that o-glcnacylation of the n-terminal region of the capsid protein of plum pox virus resembles that of animal proteins in introducing o-glcnac monomers. thr-19 and thr-24 were specifically o-glcnacylated. these residues are surrounded by amino acids typical of animal o-glcnac acceptor sites, sugge ... | 2006 | 17014851 |
| use of reverse transcription loop-mediated isothermal amplification for the detection of plum pox virus. | a one step, accelerated reverse transcription loop-mediated isothermal amplification (rt-lamp) procedure was developed for the detection of plum pox virus (ppv). the six primers required for accelerated rt-lamp were designed using a conserved region in the c-terminus of the coat protein coding region of ppv. rt-lamp was used to detect isolates of five strains of ppv including the strains d, m, ea, c, and w. the virus was detected reliably in both infected herbaceous and woody hosts. rt-lamp was ... | 2006 | 17011051 |
| self-compatible peach (prunus persica) has mutant versions of the s haplotypes found in self-incompatible prunus species. | this study demonstrates that self-compatible (sc) peach has mutant versions of s haplotypes that are present in self-incompatible (si) prunus species. all three peach s haplotypes, s (1), s (2), and s (2m), found in this study encode mutated pollen determinants, sfb, while only s (2m) has a mutation that affects the function of the pistil determinant s-rnase. a cysteine residue in the c5 domain of the s (2m)-rnase is substituted by a tyrosine residue, thereby reducing rnase stability. the peach ... | 2007 | 17006593 |
| rna silencing suppression by a second copy of the p1 serine protease of cucumber vein yellowing ipomovirus, a member of the family potyviridae that lacks the cysteine protease hcpro. | the p1 protein of viruses of the family potyviridae is a serine proteinase, which is highly variable in length and sequence, and its role in the virus infection cycle is not clear. one of the proposed activities of p1 is to assist hcpro, the product that viruses of the genus potyvirus use to counteract antiviral defense mediated by rna silencing. indeed, an hcpro-coding region is present in all the genomes of members of the genera potyvirus, rymovirus, and tritimovirus that have been sequenced. ... | 2006 | 17005683 |
| phenolic acids in berries, fruits, and beverages. | the contents of soluble and total phenolic acids were analyzed in samples of 29 berries and berry products, 24 fruits and fruit peels, and 12 beverages. variation of phenolic acids in berries was also studied. soluble phenolic acids were extracted with methanolic acetic acid, and a tentative quantification was performed by high-performance liquid chromatography (hplc). the total phenolic acid content was determined by hplc after alkaline and acid hydrolyses. the content of total phenolic acids a ... | 2006 | 16968082 |
| monocytic/macrophagic pneumonitis after intrabronchial deposition of vascular endothelial growth factor in neonatal lambs. | preterm and young neonates are prone to inadequate surfactant production and are susceptible to respiratory distress syndrome characterized by alveolar damage and hyaline-membrane formation. glucocorticoid therapy is commonly used in preterm and young infants to enhance lung maturation and surfactant synthesis. recently, vascular endothelial growth factor (vegf) was suggested to be a novel therapeutic agent for lung maturation that lacked adverse effects in mice. the purpose of this study was to ... | 2006 | 16966446 |
| novel "plum pudding" gels as potential drug-eluting stent coatings: controlled release of fluvastatin. | this study evaluated novel structural motifs known as "plum pudding" gels as potential drug-eluting stent coatings. controlled delivery of a hmg-coa reductase inhibitor (statin) from the intravascular stent surface represents a potential therapeutic modality for prevention of in-stent restenosis (isr). in this study, gels were comprised of fluvastatin-loaded thermoresponsive microgel particles containing the relatively hydrophilic n-isopropylacrylamide (nipaam), mixed with the more hydrophobic n ... | 2006 | 16941598 |
| analysis of 5-hydroxymethylfurfual in coffee, dried fruits and urine. | 5-hydroxymethylfurfural has become a substance of interest since recent results showed a possible carcinogenic potential in consequence of a metabolic activation by sulfotransferases. 5-hydroxymethylfurfural is formed either by acid catalysed degradation of reducing sugars or via the maillard reaction. this work provides an overview of foods potentially containing high amounts of 5-hydroxymethylfurfural. it comprises dried fruits with a high sugar content that were exposed to heat for a long tim ... | 2006 | 16917810 |
| characterization of the recombinant forms arising from a potato virus x chimeric virus infection under rna silencing pressure. | recombination is a frequent phenomenon in rna viruses whose net result is largely influenced by selective pressures. rna silencing in plants acts as a defense mechanism against viruses and can be used to engineer virus resistance. here, we have investigated the influence of rna silencing as a selective pressure to favor recombinants of pvx-hct, a chimeric potato virus x (pvx) vector carrying the helper-component proteinase (hc-pro) gene from plum pox virus (ppv). all the plants from two lines ex ... | 2006 | 16903356 |
| dried plum prevents bone loss in a male osteoporosis model via igf-i and the rank pathway. | previously, dietary supplementation with dried plums, a rich source of polyphenolic compounds with antioxidant and anti-inflammatory properties, has been shown to improve bone density, microstructure and biomechanics in female animal models of osteopenia. we designed this study to determine the extent to which dried plum prevents skeletal deterioration in gonadal hormone deficient male animals and to begin to understand its mechanism of action. sixty 6-month-old male sprague-dawley rats were eit ... | 2006 | 16890505 |
| [total calvarial reconstruction for sagittal synostosis]. | to study the technique of total calvaria reconstruction for sagittal synostosis. | 2006 | 16883885 |
| use of dried high-phenolic laden host leaves for virus and viroid preservation and detection by pcr methods. | the efficiency of rna extraction for apricot latent virus (aplv), plum bark necrosis stem pitting associated virus (pbnspav), prunus necrotic ring spot virus (pnrsv), potato virus y (pvy), and apple scar skin viroid (assvd) from infected hosts is of great importance for molecular diagnosis by the polymerase chain reaction (pcr). a method is described for drying tissue to overcome phenolic inhibitors of viral rna. this study showed that the infected host leaves, dried at 65 degrees c for 2 days a ... | 2006 | 16879877 |
| simultaneous release of hydrophobic and cationic solutes from thin-film "plum-pudding" gels: a multifunctional platform for surface drug delivery? | the release of two compositionally different solutes from a composite gel composed of two different populations of microgel particles embedded in a single bulk gel matrix is described, showing the potential of the "plum-pudding gel" as a multifunctional platform for controlled surface release. one hydrophobic solute (pyrene) and one hydrophobic and charged solute (rhodamine 123) were chosen as the solutes to be released. hydrophobic microgels composed of 50% n-isopropylacrylamide (nipam) and 50% ... | 2005 | 16851694 |
| detection of 6k1 as a mature protein of 6 kda in plum pox virus-infected nicotiana benthamiana. | the rna genome of plum pox virus (ppv) encodes one large polyprotein that is subsequently cleaved into mature viral proteins. one of the products of proteolytic processing, the 6k1 protein, has not yet been identified in vivo for any member of the genus potyvirus. in this study, 6k1-specific polyclonal antiserum was raised against ppv 6k1 expressed in escherichia coli as a translational fusion with the n terminus of avian troponin c and an unusual metal-binding cluster of troponin t-1. for detec ... | 2006 | 16847134 |
| transfection of arabidopsis protoplasts with a plum pox virus (ppv) infectious clone for studying early molecular events associated with ppv infection. | the development of novel strategies against plant viral diseases relies on a better understanding of molecular virus-host interactions. here, we report an easy, efficient and reproducible protocol for arabidopsis protoplast isolation and transfection to study the infection and replication of a potyvirus, plum pox virus (ppv). macerozyme and cellulose were used to release protoplasts from arabidopsis leaf tissues, and polyethylene glycol-mediated dna uptake was employed for transfection of a ppv ... | 2006 | 16777241 |
| the biosynthesis of l-rhamnose of plum-leaf polysaccharides. | 1. the utilization of d-[1-(14)c]- and d-[6-(14)c]-glucose in the biosynthesis of l-rhamnose units of plum-leaf polysaccharides has been studied. 2. after the precursors had been metabolized in the leaves, polysaccharide fractions were prepared therefrom and the constituent l-rhamnose was isolated and purified. 3. both the specific activity and the distribution of (14)c along the carbon chain of l-rhamnose from two polysaccharide fractions from each experiment were determined. 4. the results ind ... | 1965 | 16749113 |
| the role of sorbitol in the c-metabolism of the kelsey plum: relation of carbohydrate and acid loss to co(2) production in stored fruit. | 1939 | 16747117 | |
| the role of sorbitol in the carbon-metabolism of the kelsey plum: changes in chemical composition during growth and storage. | 1939 | 16747073 | |
| the nitrogen metabolism of the kelsey plum. | 1938 | 16746629 | |
| [observation on therapeutic effect of blood-letting therapy on apoplectic hemiplegia numbness syndrome]. | to observe therapeutic effect of blood-letting therapy on apoplectic hemiplegia numbness syndrome and search for clinically effective therapy. | 2006 | 16739847 |
| dna microarraying on compact disc surfaces. application to the analysis of single nucleotide polymorphisms in plum pox virus. | the potential of using compact discs as high throughput screening platforms for dna microarraying is discussed and applied to discriminate genetic variations of plum pox virus. | 2006 | 16733582 |
| the complete nucleotide sequence of the plum pox virus el amar isolate. | 2006 | 16732422 | |
| multiple resistance traits control plum pox virus infection in arabidopsis thaliana. | twelve arabidopsis accessions were challenged with plum pox potyvirus (ppv) isolates representative of the four ppv strains. each accession supported local and systemic infection by at least some of the ppv isolates, but high variability was observed in the behavior of the five ppv isolates or the 12 arabidopsis accessions. resistance to local infection or long-distance movement occurred in about 40% of all the accession-isolate combinations analyzed. except for nd-1, all accessions showed resis ... | 2006 | 16673941 |
| pyruvate orthophosphate dikinase of c(3) seeds and leaves as compared to the enzyme from maize. | pyruvate orthophosphate dikinase (ppdk) was found in various immature seeds of c(3) plants (wheat, pea, green bean, plum, and castor bean), in some c(3) leaves (tobacco, spinach, sunflower, and wheat), and in c(4) (maize) kernels. the enzyme in the c(3) plants cross-reacts with rabbit antiserum against maize ppdk. based on protein blot analysis, the apparent subunit size of ppdk from wheat seeds and leaves and from sunflower leaves is about 94 kdaltons, the same as that of the enzyme from maize, ... | 1984 | 16663632 |
| the occurrence of nitrate reductase in leaves of prunus species. | nitrate reductase was found in leaves of apricot prunus armeniaca, sour cherry p. cerasus, sweet cherry p. avium, and plum p. domestica, but not in peach p. persica, from trees grown in sand culture receiving a nitrate containing nutrient solution. nitrate was found in the leaves of all species. nitrate and nitrate reductase were found in leaves of field-grown apricot, sour cherry, and plum trees. the enzyme-extracting medium contained insoluble polyvinylpyrrolidone, and including dithiothreitol ... | 1972 | 16658037 |
| absorption, distribution, and destruction of indoleacetic acid in plum stem cuttings. | 1960 | 16655369 | |
| chronic lymphocytic leukaemia skin infiltrates affecting prominent parts of the face and the scalp. | chronic lymphocytic leukaemia (cll) infiltrating the skin is uncommon and can present in different forms. we report a case of cll infiltrating the prominent parts of the face and the scalp. a 63-year-old male with a 10-year history of cll presented with plum-coloured swelling of the skin of the ears, eyebrows, tip of the nose and the scalp. histopathology showed dense sheets of lymphoid infiltrate of the dermis which stained positive with b-cell markers cd20 and cd5 in keeping with the infiltrat ... | 2006 | 16634906 |
| asian prunus viruses: new related members of the family flexiviridae in prunus germplasm of asian origin. | serological reactivity to plum pox virus (ppv) antisera has been described in several prunus sources of asian origin that are free of ppv infection. using polyvalent or specific pcr assays, the presence of three closely related agents in two of these sources, prunus mume cv. bungo and p. persica cv. ku chu'a hung, was demonstrated. similarities in genome organization and sequence comparisons indicate that these agents should be regarded as members of the genus foveavirus, their only singular tra ... | 2006 | 16621102 |
| identification of a plum pox virus ci-interacting protein from chloroplast that has a negative effect in virus infection. | the cylindrical inclusion (ci) protein of potyviruses is involved in virus replication and cell-to-cell movement. these two processes should rely on multiple plant-virus interactions; however, little is known about the host factors that are involved in, or that may interfere with, ci functions. by using a yeast two-hybrid system, the ci protein from plum pox virus (ppv) was found to interact with the photosystem i psi-k protein, the product of the gene psak, of nicotiana benthamiana. coexpressio ... | 2006 | 16570664 |
| plum pox virus induces differential gene expression in the partially resistant stone fruit tree prunus armeniaca cv. goldrich. | we investigated the changes in the expression profiles of the partially resistant apricot (prunus armeniaca l.) cultivar goldrich following inoculation with plum pox virus (ppv) using cdna-amplification fragment length polymorphism (aflp). altered expression patterns were detected and twenty-one differentially expressed cdna had homologies with genes in databases coding for proteins involved in metabolism, signal transduction, defense, stress and intra/intercellular connections. seven of the mod ... | 2006 | 16564142 |
| the cover. branche de prunier, fond vert (plum blossoms, green background). | 2006 | 16537726 | |
| diversity of opines and opine-catabolizing bacteria isolated from naturally occurring crown gall tumors. | the diversity of opines from 43 naturally occurring crown gall tumors on several plant species was analyzed for the presence of agropine, chrysopine, iminodiacid, an unidentified leucinopine-like iminodiacid (ida-b), mannopine, octopine, nopaline, dl- and ll-succinamopine, leucinopine and heliopine. opine utilization patterns of agrobacteria and fluorescent pseudomonads resident in a tumor were then analyzed and compared for agreement with the opine isolated from that tumor. nopaline was the mos ... | 1997 | 16535484 |
| calibration of sap flow estimated by the compensation heat pulse method in olive, plum and orange trees: relationships with xylem anatomy. | the compensation heat pulse method is widely used to estimate sap flow in conducting organs of woody plants. being an invasive technique, calibration is crucial to derive correction factors for accurately estimating the sap flow value from the measured heat pulse velocity. we compared the results of excision and perfusion calibration experiments made with mature olive (olea europaea l. 'manzanilla de sevilla'), plum (prunus domestica l. 'songal') and orange (citrus sinensis (l.) osbeck. 'cadener ... | 2006 | 16510387 |
| ppv long-distance movement is occasionally permitted in resistant apricot hosts. | the interactions between plum pox virus (ppv), a member of the potyvirus genus, and prunus host plants are, up to now, poorly understood. in the current paper, fluorescence stereomicroscopy, in situ hybridisation and immunogold detection were performed in order to evaluate the virus transport and cellular distribution. the behavior of ppv in several susceptible (cv. "moniqui" and "screara") and resistant apricot genotypes (cv. "harlayne", "henderson", "harcot", "goldrich", "stella" and "stark ea ... | 2006 | 16504333 |
| gamma-irradiated dry fruits. an example of a wide variety of long-time dependent epr spectra. | epr spectra of dry, sugar containing fruits--raisins, sultanas, figs, dates, peaches, blue plums and chokeberry recorded before and after irradiation with gamma-rays, are reported. it is shown that weak singlet epr line with 2.0031+/-0.0005 can be recorded before irradiation of seeds, stones or skin of chokeberry, figs and raisins as well as flesh of blue plum, raisins and peaches. epr signals of various shape are distinguished after irradiation in different parts of the fruits, as well as in ra ... | 2006 | 16497546 |
| microrna-guided processing impairs plum pox virus replication, but the virus readily evolves to escape this silencing mechanism. | since the discovery of microrna (mirna)-guided processing, a new type of rna silencing, the possibility that such a mechanism could play a role in virus defense has been proposed. in this work, we have analyzed whether plum pox virus (ppv) chimeras bearing mirna target sequences (mir171, mir167, and mir159), which have been reported to be functional in arabidopsis, were affected by mirna function in three different host plants. some of these ppv chimeras had clearly impaired infectivity compared ... | 2006 | 16474149 |
| distinct viral populations differentiate and evolve independently in a single perennial host plant. | the complex structure of virus populations has been the object of intensive study in bacteria, animals, and plants for over a decade. while it is clear that tremendous genetic diversity is rapidly generated during viral replication, the distribution of this diversity within a single host remains an obscure area in this field of science. among animal viruses, only human immunodeficiency virus and hepatitis c virus populations have recently been thoroughly investigated at an intrahost level, where ... | 2006 | 16474141 |
| peacock or plum? reflections on academic research career pathways. | purpose of the paper: this paper critically deconstructs academic career pathways in research and explores possible future directions. | 2006 | 16458998 |
| blackberry (rubus spp.): a ph-dependent oral contrast medium for gastrointestinal tract images by magnetic resonance imaging. | in this study, seven fruits have been tested on their magnetic properties, paramagnetic metal content and contrast enhancement in magnetic resonance imaging (mri) of phantom and in vivo. magnetic susceptibility was determined for the fruit pulps, as well as the contents of paramagnetic metals; iron, manganese and copper. the total content of these metals was 4.3, 8.6, 11.1, 10.9, 12.3, 8.3 and 29.3 mg/kg of fruit for plum, blueberry, apple (red), pineapple, beet, grape, blackberry, respectively, ... | 2006 | 16455409 |
| questions location of plum island facility. | 2006 | 16426161 | |
| [observation on therapeutic effect of plum-blossom needle therapy on peripheral facial neuritis at acute stage]. | to compare difference between plum-blossom needle therapy and hormonotherapy in the the treatment group of 32 cases with periphtherapeutic effect on peripheral facial neuritis at acute stage. | 2005 | 16419706 |
| inhibitory effects of fruit juices on cyp3a activity. | there have been very limited reports on the effects of commercial fruit juices on human cyp3a activity. therefore, the inhibitory effects of readily available commercial fruit juices on midazolam 1'-hydroxylase activity, a marker of cyp3a, were evaluated in pooled human liver microsomes. the fruit juices investigated were black raspberry, black mulberry, plum, and wild grape. white grapefruit, pomegranate, and orange juice were used as positive and negative controls. the black mulberry juice sho ... | 2006 | 16415112 |
| pesticides and metabolites in cassava, eucalyptus, plum and cashew leaves and roots in relation to a point source in kibaha, tanzania. | leaves of eucalyptus sp., prunus domestica (plum), and anacardium occidentale (cashew), as well as roots and leaves of manihot esculenta (cassava) were used to study the local distribution of pesticides and metabolites from a point source, an old storage site at vikuge farm in tanzania. the gpc-cleaned extracts were analyzed by gc-ecd and gc/ms. eleven organochlorine pesticide residues namely pentachloroanisole, p,p'-ddt, o,p'-ddt, p,p'-dde, o,p'-dde, p,p'-ddd, o,p'-ddd, alpha-hch, beta-hch, del ... | 2006 | 16403548 |
| the complete genome sequence of an el amar isolate of plum pox virus (ppv) and its phylogenetic relationship to other ppv strains. | the genomic sequence of an el amar isolate of plum pox virus (ppv) from egypt was determined by sequencing overlapping cdna fragments. this is the first complete sequence of a member of the el amar (ea) strain of ppv. the genome consists of 9791 nt, excluding a poly(a) tail at the 3' terminus. the complete nt sequence of ppv ea is 79-80%, 80%, 77%, and 77% homologous with isolates of strains d/m, rec (bor3), c, and w, respectively. the polyprotein identity ranged from 87-91%. phylogenetic analys ... | 2006 | 16397750 |
| characterization and partial genome sequence of stocky prune virus, a new member of the genus cheravirus. | characterization of a seemingly new spherical virus isolated from severely affected plum trees in south-western france indicated that its divided genome is composed of two single-stranded, polyadenylated rnas of approximately 7.4 and 3.7 kb. its particles are composed of three coat protein subunits of approximately 23, 23.5, and 24.5 kda. partial sequencing of the genomic rnas indicated that this new virus, tentatively named stocky prune virus (stpv), is distantly related to the two sequenced ch ... | 2006 | 16380812 |
| flower fertilization and fruit development prompt changes in free polyamines and ethylene in damson plum (prunus insititia l.). | the flower opening of damson plum (prunus insititia l.) was accompanied by an increase in the content of free-polyamines (pa) in the sepals, petals and sex organs, the ovary being most active in accumulating spermine (spm). the fertilization process and senescence brought on a decline in ovarian spm, but stimulated putrescine (put) and spermidine (spd) content in the sepals. the endocarp of this climacteric fruit produced only ethylene at the end of the s1 phase and throughout s2, in which there ... | 2006 | 16360807 |
| usefulness of diurnal trunk shrinkage as a water stress indicator in plum trees. | we compared seasonal changes in maximum diurnal trunk shrinkage (mds) with seasonal changes in midday stem water potential (psi(s)) over three years in plum trees grown in differing drip-irrigated regimes. in well-irrigated trees, day-to-day variations in psi(s) and mds were related to evaporative demand. reference equations were obtained to predict mds and psi(s) values for well-irrigated trees as functions of environmental conditions. a decrease in plant water status toward the end of the grow ... | 2006 | 16356902 |
| effect of dried plums on colon cancer risk factors in rats. | dried plums (that is, prunes) are a fruit that show promise as a food to lower colon cancer risk, based on their high content of dietary fiber and polyphenolics. in this study, we have examined the effect of diets containing dried plums on the number of colonic precancerous lesions (aberrant crypts, acs), fecal bile acid concentration, and cecal bacterial enzyme activities related to colon cancer risk. rats were fed one of four diets: a basal diet (a modified ain-93g diet), a low-concentration d ... | 2005 | 16351514 |
| evidence of biological control of agrobacterium tumefaciens strains sensitive and resistant to agrocin 84 by different agrobacterium radiobacter strains on stone fruit trees. | the effectiveness of agrobacterium radiobacter k84, 0341, and a k84 non-agrocin-producing mutant (k84 agr) in biological control of crown gall on rootstocks of stone fruit trees was determined in three experiments. in experiment 1, k84 and 0341 controlled crown gall on plum plants in soil inoculated with two strains of agrobacterium tumefaciens resistant to agrocin 84. in experiment 2, k84 controlled crown gall on peach plants in soils inoculated with strains of a. tumefaciens sensitive or resis ... | 1989 | 16347881 |
| medium for isolation and growth of bacteria associated with plum leaf scald and phony peach diseases. | rickettsia-like bacteria associated with plum leaf scald and phony peach diseases were isolated from diseased but not from healthy tissues and cultured on charcoal-yeast extract medium (bcye) buffered with aces (2-[(2-amino-2-oxoethyl) amino]-ethanesulfonic acid). optimum conditions for isolation and growth on bcye medium were ph 6.5 to 6.9 at 20 and 25 degrees c under normal atmosphere. growth of primary colonies and first-passage subcultures was restricted, and colonies reached a maximum diame ... | 1981 | 16345835 |
| [clinical observation on plum-blossom needle therapy combined with cupping for treatment of acute facial paralysis]. | to observe clinical therapeutic effect of plum-blossom needle therapy combined with cupping at the affected side on acute facial paralysis. | 2005 | 16335199 |
| aphid (hemiptera: aphididae) species composition and potential aphid vectors of plum pox virus in pennsylvania peach orchards. | plum pox, an invasive disease recently identified in pennsylvania stone fruit orchards, is caused by the aphid-transmitted plum pox virus (genus potyvirus, family potyviridae, ppv). to identify potential vectors, we described the aphid species communities and the seasonal dynamics of the dominant aphid species within pennsylvania peach orchards. aphids were trapped weekly in 2002 and 2003 from mid-april through mid-november within two central pennsylvania orchards by using yellow and green water ... | 2005 | 16334309 |
| hairpin rna-mediated silencing of plum pox virus p1 and hc-pro genes for efficient and predictable resistance to the virus. | we report the application of the hairpin-mediated rna silencing technology for obtaining resistance to plum pox virus (ppv) infection in nicotiana benthamiana plants. four sequences, covering the p1 and silencing suppressor hc-pro genes of an italian ppv m isolate, were introduced into n. benthamiana plants as two inverted repeats separated by an intron sequence under the transcriptional control of the cauliflower mosaic virus 35s promoter. in a leaf disk infection assay, 38 out of 40 t0 transge ... | 2005 | 16315100 |
| [treatment of 58 cases of vitiligo by electro-plum-blossom needle therapy combined with catgut implantation at acupoints under tdp radiation]. | to search for a method for increasing therapeutic effect on vitiligo. | 2005 | 16312885 |
| [comparison of therapeutic effects of acupuncture and moxibustion on diabetic peripheral neuropathies]. | to observe and compare therapeutic effects of acupuncture, acupoint injection and tapping with plum-blossom needle and its influences on the nerve conduction velocity in the patient of diabetic peripheral neuropathies. | 2005 | 16309050 |
| anti-hyperglycemic effects of plum in a rat model of obesity and type 2 diabetes, wistar fatty rat. | dried plums, considered a healthy food in the west and used as medicine in india, contain phenolic compounds with protective actions against age-related diseases. effects of oral plum ekisu (concentrated juice) on lipid and glucose tolerance were assessed in insulin-resistant obese wistar fatty rats. plum ingestion decreased blood glucose (p < 0.05) and plasma triglyceride concentrations (p < 0.01) compared with controls. plum treatment for 2 weeks reduced areas under the curve (aucs) for glucos ... | 2005 | 16295695 |
| real-time rt-pcr and sybr green i melting curve analysis for the identification of plum pox virus strains c, ea, and w: effect of amplicon size, melt rate, and dye translocation. | real-time rt-pcr and sybr green i melt curve analysis of a 74 bp amplicon enabled identification of plum pox virus strains c, ea, and w, with distinct t(m)'s associated with each strain. this test is a useful supplement to a real-time rt-pcr test described earlier that was used to distinguish ppv strains d and m. a longer fragment of 155 bp was not effective for strain identification. a simplified one-tube protocol, with dithiothreitol eliminated from the reaction, showed similar sensitivity whe ... | 2006 | 16293321 |
| [relationship between pollen allergy and oral allergy syndrome]. | we recently evaluated the relationship between pollen allergy and oral and pharyngeal hypersensitivity to fruits and vegetables (oral allergy syndrome) over 8 years (1995 approximately 2002) in sapporo. 1. of 843 patients with birch pollen (bp) allergy (cap score 2 or more), 378 patients (37%) had episodes of oral allergy syndrome (oas), and the rate in bp allergy was higher than in other allergies. 2. in patients with bp allergy, the most frequent foods causing oas were apple, peach and cherry, ... | 2005 | 16285612 |
| dried plum reverses bone loss in an osteopenic rat model of osteoporosis. | we previously reported the efficacy of dried plum (prunus domestica l.) in preventing ovariectomy-induced bone loss in a rat model of osteoporosis and improving bone biomarkers in postmenopausal women. the present study evaluated whether dried plum was able to restore bone mass in osteopenic ovariectomized rats. | 2005 | 16278620 |
| yellow pigments used in rapid identification of aflatoxin-producing aspergillus strains are anthraquinones associated with the aflatoxin biosynthetic pathway. | studies on biological control of aflatoxin production in crops by pre-infection with non-toxigenic aspergillus flavus strains have created a need for improved methods to screen isolates for aflatoxigenicity. we have evaluated two empirical aflatoxigenicity tests: (i) yellow pigment production, and (ii) the appearance of a plum-red color in colonies exposed to ammonium hydroxide vapor. yellow pigments from aflatoxigenic a. flavus were shown to function as ph indicator dyes. seven pigments represe ... | 2005 | 16260026 |
| genetic analysis of the function of the plum pox virus ci rna helicase in virus movement. | the ci protein forms the cylindrical inclusions typical of potyviral infections and is involved in genome replication and virus movement. in this work, we have analyzed the effect of a series of point mutations at the n-terminal region of the ci protein of plum pox virus (ppv) on the enzymatic activities and the self-interaction ability of the protein, and on virus replication and movement. dd3,4aa mutation, which had no apparent effects on atpase and rna helicase activities in vitro, and on vir ... | 2006 | 16256236 |
| [geostatistics analysis on spatial patterns of myzus persicae and erigonidium graminicola in plum orchard]. | investigations on the spatial construction and distribution of myzus persicae and erigonidium graminicola in a plum orchard were conducted from march 2003 to november 2003. the results indicated that the semivariogram of myzus persicae could be described by spherical model, except on june 27 and november 22, which should be described by lined model, and that of erigonidium graminicola could be described by spherical model, except on may 21, may 31, october 19 and november 22, which should be des ... | 2005 | 16252873 |
| treatment of hypersaline-containing wastewater with salt-tolerant microorganisms. | two salt-tolerant bacteria were isolated from soil samples to treat a high-salt-content wastewater from a pickled plum production plant. the strains, which were identified as staphylococcus sp. and bacillus cereus, grew well in a medium containing 0 to 15% nacl. when wastewater in a flask was treated with the strains for 72 h, the cod removal was about 70%, and this was increased to about 90% when they were used in a pilot plant (working volume 1 m3) in a batch culture carried out for 7 d. the s ... | 2001 | 16232979 |
| u.s. plans to modernize plum island facility. | 2005 | 16220662 | |
| effect of the stone content on the quality of plum and cherry spirits produced from mash fermentations with commercial and laboratory yeast strains. | to evaluate the influence of stone content on spirit quality from stone fruit, cherry and plum mashes were prepared and fermented with a commercial and a diploid laboratory yeast strain. fermentation parameters such as sugar content and ethanol production were followed. despite an initial lag phase in cherry spirits, both yeast strains performed similarly, as substantiated by the determination of specific flavor compounds, ethyl carbamate, and methanol in the mashes and after distillation. the s ... | 2005 | 16218669 |
| role of host feeding niches and host refuges in habitat-related behaviour of hyssopus pallidus (hymenoptera: eulophidae), a larval parasitoid of the codling moth. | parasitoid fitness depends largely on the capability to locate a host in an ecosystem. a parasitoid of a polyphagous host might not be able to find or to access the host in all its feeding niches. this study evaluated the niche selection of hyssopus pallidus (askew), a larval parasitoid of cydia pomonella (linnaeus), at the plant level with the goal of assessing its potential for biological control on different fruit crops throughout the plant cycle. parasitoid behaviour during host location and ... | 2005 | 16197563 |
| [ecological effects of intercrops in comprehensive management patterns of hilly area plum orchard]. | the study on the ecological effects of intercrops in four cropping patterns showed that with the increase of multiple cropping, the annual increase of soil organic matter, total n, total p and total k was 5% - 20%, 7% - 40%, 8% - 70% and 15% - 80%, respectively. potato-soybean had the best benefit in soil and water conservation, followed by cole-peanut, potato-watermelon, and wheat. compared with control, the average soil erosion module and runoff amount of 4 patterns were decreased by 44.19%, 3 ... | 2005 | 16180746 |
| one hundred and eighty-nine cases of acute articular soft tissue injury treated by blood-letting puncture with plum-blossom needle and cupping. | 2005 | 16136937 | |
| ulcerative colitis treated by acupuncture at jiaji points (ex-b2) and tapping with plum-blossom needle at sanjiaoshu (bl22) and dachangshu (bl 25)--a report of 43 cases. | 2005 | 16136930 | |
| accumulation of the long class of sirna is associated with resistance to plum pox virus in a transgenic woody perennial plum tree. | we investigated the hallmarks of posttranscription gene silencing (ptgs) in mature plants, embryos, and seedlings of the transgenic plum trees (prunus sp.) that are resistant to plum pox virus (ppv). we previously demonstrated that the transgene insert and resistance to ppv were mutually inherited in progeny of line c5. we show here that c5 constitutively produces a short (22 nt) and a long (25 to 26 nt) species of short interfering (si)rna from embryo to mature plant in the absence of ppv inocu ... | 2005 | 16134891 |
| strain variability in the dna immigration control region (icr) of xylella fastidiosa. | the genome of the bacterium xylella fastidiosa contains four orfs (xf2721, xf2725, xf2739 and xf0295) related to the restriction modification type i system, ordinarily named r-m. this system belongs to the dna immigration control region (icr). each orf is related to different operon structures, which are homologues among themselves and with subunit hsd r from the endonuclease coding genes. in addition, these orfs are highly homologous to genes in pseudomonas aeruginosa, methylococcus capsulatus ... | 2006 | 16125907 |
| magnetic resonance imaging of the small bowel in crohn's disease. | there may be advantages in using magnetic resonance imaging (mri) in small-bowel disease. the aims of this study were to optimize the mri examination technique and to evaluate the capabilities of mri compared with those of conventional enteroclysis (ce). | 2005 | 16109660 |
| loss of diversity of ammonia-oxidizing bacteria correlates with increasing salinity in an estuary system. | ammonia-oxidizing bacteria (aob) play an important role in nitrogen cycling in estuaries, but little is known about aob diversity, distribution and activity in relation to the chemical and physical changes encountered in estuary systems. although estuarine salinity gradients are well recognized to influence microbial community structure, few studies have examined the influence of varying salinity on the diversity and stability of aob populations. to investigate these relationships, we collected ... | 2005 | 16104852 |
| influence of selected fruit tree pollen on life history of euseius stipulatus (acari: phytoseiidae). | euseius stipulatus (athias-henriot) is a predatory mite widespread in the mediterranean region considered to be important for the biological control of spider mites in citrus orchards. development, survival and reproduction of this phytoseiid mite feeding on seven commercially obtained pollen were studied under constant laboratory conditions (20 +/- 1 degrees c, rh 65 +/- 5%, photoperiod 16l: 8d h). mites were kept individually at rearing units with ample quantity of almond (prunus amygdalus bat ... | 2005 | 16082919 |
| real-time pcr for simultaneous and quantitative detection of quarantine phytoplasmas from apple proliferation (16 srx) group. | a real time pcr assay conjugated with the fluorescent sybr green i dye has been developed for rapid, sensitive and quantitative detection of 'ca. phytoplasma pyri', 'ca. p. prunorum' and 'ca. p. mali', quarantine members of apple proliferation (16 srx) group. the selected primers amplify specifically a target of 217-bp fragment from the 16 sr gene region of the 16 srx group and not from any other tested phytoplasma groups. an artificial template consisting in a plasmid clone of a 1785-bp dna fra ... | 2005 | 16039094 |
| retrospective trends and current status of ethyl carbamate in german stone-fruit spirits. | ethyl carbamate (urethane, c(2)h(5)oconh(2)) is a known genotoxic carcinogen of widespread occurrence in fermented food and beverages with highest concentrations found in stone-fruit spirits. between 1986 and 2004, 631 cherry, plum or mirabelle (yellow plum) spirits were analysed for ethyl carbamate using gas chromatography in combination with mass spectrometry after extrelut extraction. the ethyl carbamate concentration of the samples ranged between 0.01 mg l(-1) and 18 mg l(-1) (mean 1.4 mg l( ... | 2005 | 16019810 |
| identification of secret agent as the o-glcnac transferase that participates in plum pox virus infection. | serine and threonine of many nuclear and cytoplasmic proteins are posttranslationally modified with o-linked n-acetylglucosamine (o-glcnac). this modification is made by o-linked n-acetylglucosamine transferases (ogts). genetic and biochemical data have demonstrated the existence of two ogts of arabidopsis thaliana, secret agent (sec) and spindly (spy), with at least partly overlapping functions, but there is little information on their target proteins. the n terminus of the capsid protein (cp) ... | 2005 | 16014901 |
| [cluster analysis on the temporal dynamics of arthropod community in a plum orchard]. | in this paper, twelve investigations were conducted on the structural feature of arthropod community in a plum orchard, and a cluster analysis was made on the temporal dynamics of the community. the total community could be clustered into 5 clusters (d = 0.2000), i.e., that in march, in june, in july, in november, and in other months. the natural enemy and pest-neutral sub-communities could be also clustered into 5 clusters, respectively. for natural enemy sub-community, the clusters (d = 0.2000 ... | 2005 | 16011157 |
| determination of malondialdehyde (mda) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stress. application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants from fruits. | a high-performance liquid chromatography (hplc) method to determine malondialdehyde (mda) as the 2,4-dinitrophenylhydrazine (dnph) derivative was applied to biological samples (serum and liver homogenates). since mda is considered a presumptive biomarker for lipid peroxidation in live organisms, a model for nutritionally induced oxidative stress (hypercholesterolemic rats) was studied in comparison with normocholesterolemic animals. the effect of diet supplementation with fruits rich in antioxid ... | 2005 | 16009604 |
| blue rubber bleb naevus syndrome associated with cortical blindness. | an 83-year-old woman was admitted for investigation of sudden loss of vision. extensive plum-purple papules and nodules involved her skin and lips but not mucosae. a cutaneous biopsy demonstrated irregular vascular cavernous channels in the dermis and subcutis; deeper vessels displayed smooth muscle. blue rubber bleb naevus syndrome was diagnosed. this patient had no gastrointestinal symptoms, no family history of blue rubber bleb naevus syndrome, and only developed cutaneous signs after 60 year ... | 2005 | 16008655 |
| [fertility of large fruit-tree tortrix archips podana scop. (lepidoptera: tortricidae) on different edible plants]. | we studied pupal weight, total fertility, and potential and actual fertility of imagoes of large fruit-tree tortrix archips podana scop. for caterpillars reared on apple, currant, pear, lilac, and plum plants. pupal weight had no effect on total fertility in the first generation but was reflected in the second generation. the dependence of potential and actual fertility of imago on pupal weight was nonlinear with a trend to optimum within certain limits. | 2005 | 16004289 |
| a multigene phylogenetic study of clonal diversity and divergence in north american strains of the plant pathogen xylella fastidiosa. | xylella fastidiosa is a pathogen that causes leaf scorch and related diseases in over 100 plant species, including pierce's disease in grapevines (pd), phony peach disease (pp), plum leaf scald (pls), and leaf scorch in almond (als), oak (oak), and oleander (ols). we used a high-resolution dna sequence approach to investigate the evolutionary relationships, geographic variation, and divergence times among the x. fastidiosa isolates causing these diseases in north america. using a large data set ... | 2005 | 16000795 |
| application of gfp-tagged plum pox virus to study prunus-ppv interactions at the whole plant and cellular levels. | the sharka disease caused by the potyvirus plum pox virus (ppv) is one of the most serious viral diseases affecting stone fruit trees. the study of ppv/prunus interaction under greenhouse controlled conditions is space, time, labor consuming. while the ppv/prunus interactions are now quite well known at the whole plant level, few data however are available on the interactions between the virus and the prunus host plants at the cellular level. using a green fluorescent protein (gfp)-tagged m type ... | 2005 | 15993953 |
| real-time assay for quantitative detection of non-persistently transmitted plum pox virus rna targets in single aphids. | a taqman real-time rt-pcr was developed to detect and quantify rna-targets from the non-circulative, non-persistently transmitted plum pox virus (ppv) in individual fresh or aphids captured previously and squashed on paper. reliable quantitation ranged from 40 up to 4 x 10(8) copies of control transcripts. this technique was applied successfully to plant material and to individual ppv vector (myzus persicae) and non-vector of ppv (aphis nerii) aphid species demonstrating acquisition of viral tar ... | 2005 | 15964640 |
| analysis of recombinant plum pox virus (ppv) isolates from serbia confirms genetic homogeneity and supports a regional origin for the ppv-rec subgroup. | the recent observation of the frequent occurrence of natural recombinant plum pox virus (ppv) isolates has led to the identification of a distinct ppv subgroup, named ppv-rec. the diversity, origin and geographical spread of the recombinant ppv isolates belonging to this subgroup remain, however, relatively poorly known. in an effort to further our understanding of these isolates, eight ppv isolates from serbia, the country from which the first such recombinant (ppv-o6) originated, were characte ... | 2005 | 15906106 |
| cx31 and cx43 double-deficient mice reveal independent functions in murine placental and skin development. | the overlapping expression of gap junctional connexins in tissues has indicated that the channels may compensate for each other. during development, cx31 and cx43 are coexpressed in preimplantation embryos, in the spongiotrophoblast of the placenta and in the epidermis. this study shows that cx31/cx43 double-deficient mice exhibit the known phenotypes of the single-knockout strains but no combined effects. thus, cx43, coexpressed with cx31 at midgestation in the spongiotrophoblast of the placent ... | 2005 | 15895417 |
| gender difference in hiv-1 rna viral loads. | to test and characterize the dependence of viral load on gender in different countries and racial groups as a function of cd4 t-cell count. | 2005 | 15876283 |
| synthetic ion channels with rigid-rod pi-stack architecture that open in response to charge-transfer complex formation. | we describe the design, synthesis, and evaluation of synthetic ion channels with a new rigid-rod pi-stack architecture that open in response to guest binding by aromatic electron donor-acceptor interactions. highly cooperative and highly selective ligand gating is shown to yield anion selective, small ion channels that have the characteristic plum color of the charge-transfer complexes formed between the dialkoxynaphthalene ligands and the stacked naphthalenediimide acceptors of the channel. | 2005 | 15869262 |
| novel reference gene, high-mobility-group protein i/y, used in qualitative and real-time quantitative polymerase chain reaction detection of transgenic rapeseed cultivars. | with the development of transgenic crops, regulations to label the genetically modified organisms (gmos) and their derived products have been issued in many countries. polymerase chain reaction (pcr) methods are thought to be reliable and useful techniques for qualitative and quantitative detection of gmos. these methods are generally needed to amplify the transgene and compare the amplified results with that of a corresponding reference gene to get the reliable results. specific primers were de ... | 2005 | 15859086 |
| comparison of the antioxidant activities of nine different fruits in human plasma. | oxidative stress in humans is associated with damage to dna, proteins, and biological membranes. oxidative stress, which often arises as a result of an imbalance in the human antioxidant status, has been implicated in aging and a number of human diseases such as cancer, atherosclerosis, and rheumatoid arthritis. this study was performed to test the hypothesis that the consumption of fruit juices may improve antioxidant status in human plasma. ten healthy men 25-26 years old were recruited for th ... | 2005 | 15857208 |
| [characteristics, dynamics and niche of insect community in plum orchard]. | the insect community in plum orchard was investigated on organization level and temporal-spatial niche. the results showed that the insect community was abundant, which included 6 orders, 23 families. the individuals of species, diversity indices, and evenness increased with time. myzus persicae and asiaarposina sasokii had the widest spatial niche breadth, while didesmococcus koreauus borchs had the widest temporal niche breadth. among the natural enemies, chilocorus rubidus had the widest both ... | 2005 | 15852928 |
| nucleotide sequence analysis of plum pox virus isolate w3174: evidence of a new strain. | the nucleotide sequence of plum pox virus (ppv) isolate w3174 was determined. the virus genome consists of 9788 nucleotides (nt), excluding the poly(a) tail at the 3'-terminus, with 5'- and 3'-untranslated regions (utrs) of 146 and 219 nt, respectively. the deduced polyprotein consists of 3141 amino acid (aa) residues, with the coat protein region consisting of 993 nt (331 aa). in comparisons with ppv-d, -m, -ea and -c isolates, nucleotide identity levels ranged from 79 to 80% for the entire gen ... | 2005 | 15845265 |
| genomic variability of prune dwarf virus as affected by agricultural practice. | twelve new sequences of the coat protein gene of prune dwarf virus (pdv) variants, obtained from almond trees, are presented. comparison with previously reported sequences of the same region, obtained from other hosts (plum, cherry and peach) revealed not only the existence of a wider range of variants of pdv than formerly predicted, but also the frequent presence of a mixture of variants in each sample. in spite of the heterogeneity found in almond, the amino acid composition of the domain at t ... | 2005 | 15789267 |
| spermatophore deposition throughout the day by the plum rust mite, aculus fockeui. | 2005 | 15777004 | |
| characterization of pseudomonas pathovars isolated from rosaceous fruit trees in east algeria. | a survey of bacterial diseases due to pseudomonas on rosaceous fruit trees was conducted. in forty two orchards located in the constantine region ( east algeria). pseudomonas isolates were identified on the bases of their cultural and biochemical characteristics . a total of fifty nine phytopathogenic bacteria were isolated from diseased pome and stone fruit trees. thirty one strains comparable to pseudomonas syringae pv. syringae were isolated from cherry (prunus avium l.), plum (p. domestica l ... | 2004 | 15756824 |
| properties of a virus isolated from vernonia amygdalina del. in lagos, nigeria. | a previously uncharacterized virus tentatively named vernonia green vein-banding virus (vgvbv) was isolated from vernonia amygdalina del. ("bitterleaf") from lagos, nigeria. the virus was mechanically transmissible but had a narrow host range restricted to nicotiana benthamiana, chenopodium quinoa and c. amaranticolor. it was also transmissible in a non-persistent manner by myzus persicae. the virus was purified from n. benthamiana and about 750 nm long flexuous rod-shaped particles were observe ... | 2004 | 15745049 |
| comparative effects of food-derived polyphenols on the viability and apoptosis of a human hepatoma cell line (hepg2). | consumption of fruits and vegetables, which are rich in polyphenols, has been associated with a reduced risk of chronic diseases such as cancer. dietary polyphenols have antioxidant and antiproliferative properties that might explain their beneficial effect on cancer prevention. the aim of this study was to investigate the effects of different pure polyphenols [quercetin, chlorogenic acid, and (-)-epicatechin] and natural fruit extracts (strawberry and plum) on viability or apoptosis of human he ... | 2005 | 15713052 |
| [inhaled foreign bodies in 50 patients in south vietnam, mainly caused by naseberry (sapodilla)]. | inhalation of foreign bodies is relatively frequent in children, but exceptional in adults. various kinds of foreign bodies can be inhaled, the type generally depends on eating habits in adults. we reviewed a series of 50 consecutive cases of inhaled foreign bodies and found that naseberry fruits (sapodilla plum) was the primary cause, followed by bone debris. this series is typical of eastern asia, particularly south vietnam. most of the foreign bodies were extracted under local anesthesia usin ... | 2004 | 15699907 |