Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| isolation and characterization of a gs2 gene in melon (cucumis melo l.) and its expression patterns under the fertilization of different forms of n. | we isolated a novel glutamine synthetase (gs, ec 6.3.1.2) gene m-gs2 (accession: ay773090) by the race approach from melon. the full-length cdna of m-gs2 is 1807 bp and contains a 1296 bp open reading frame (orf) encoding 432 amino acids. the deduced protein contains conserved structural domains among plant gs2 proteins and shares extensive sequence homology with gs2 enzymes from other higher plants. m-gs2 expresses with specificity in leaf, and identification of a chloroplast transit peptide (c ... | 2010 | 19669951 |
| a set of est-snps for map saturation and cultivar identification in melon. | there are few genomic tools available in melon (cucumis melo l.), a member of the cucurbitaceae, despite its importance as a crop. among these tools, genetic maps have been constructed mainly using marker types such as simple sequence repeats (ssr), restriction fragment length polymorphisms (rflp) and amplified fragment length polymorphisms (aflp) in different mapping populations. there is a growing need for saturating the genetic map with single nucleotide polymorphisms (snp), more amenable for ... | 2009 | 19604363 |
| a conserved ethylene biosynthesis enzyme leads to andromonoecy in two cucumis species. | andromonoecy is a widespread sexual system in angiosperms, characterized by plants carrying both male and bisexual flowers. monoecy is characterized by the presence of both male and female flowers on the same plant. in cucumber, these sexual forms are controlled by the identity of the alleles at the m locus. in melon, we recently showed that the transition from monoecy to andromonoecy result from a mutation in 1-aminocyclopropane-1-carboxylic acid synthase (acs) gene, cmacs-7. to isolate the and ... | 2009 | 19578542 |
| a consensus linkage map identifies genomic regions controlling fruit maturity and beta-carotene-associated flesh color in melon (cucumis melo l.). | the nutritional value and yield potential of us western shipping melon (usws; cucumis melo l.) could be improved through the introgression of genes for early fruit maturity (fm) and the enhancement of the quantity of beta-carotene (qbetac) in fruit mesocarp (i.e., flesh color). therefore, a set of 116 f(3) families derived from the monoecious, early fm chinese line 'q 3-2-2' (no beta-carotene, white mesocarp) and the andromonoecious, late fm usws line 'top mark' (possessing beta-carotene, orange ... | 2009 | 19551368 |
| superoxide dismutase activity in mesocarp tissue from divergent cucumis melo l. genotypes. | muskmelons (cucumis melo l.) are well-known as excellent sources of several vitamins, minerals and non-enzymatic antioxidant phytochemicals such as vitamin c and pro-vitamin a. less well-studied is their potential role as sources of enzymatic antioxidants such as superoxide dismutase (sod), which have been associated with enhanced reactive oxygen species scavenging capacity in some muskmelon fruits. in this study, we investigated the variability in sod activities among diverse advanced breeding ... | 2009 | 19529987 |
| influence of thermal and dense-phase carbon dioxide pasteurization on physicochemical properties and flavor compounds in hami melon juice. | the influence of thermal and dense-phase carbon dioxide (dp-co(2)) pasteurization on physicochemical properties and flavor compounds in hami melon juice was investigated. melon juice was pasteurized using dp-co(2) treatment and compared to a conventional high-temperature-short-time (htst) method. the dp-co(2) treatment was carried out using a dp-co(2) unit (55 degrees c, 60 min, and 35 mpa). the thermal pasteurization was performed at 90 degrees c for 60 s with an adapted laboratory setup. effec ... | 2009 | 19522496 |
| sensitivity to azoxystrobin in didymella bryoniae isolates collected before and after field use of strobilurin fungicides. | isolates of didymella bryoniae (auersw.) rehm, causal agent of gummy stem blight on cucurbits, developed insensitivity to azoxystrobin in the eastern united states 2 years after first commercial use in 1998. baseline sensitivity of this fungus to azoxystrobin has never been reported. the objectives were to compare baseline sensitivities of d. bryoniae from south carolina and other locations to sensitivities of isolates exposed to azoxystrobin for one or more seasons, and to compare sensitivity i ... | 2009 | 19488995 |
| generation of transgenic oriental melon resistant to zucchini yellow mosaic virus by an improved cotyledon-cutting method. | production of melon (cucumis melo l.) worldwide is often limited by the potyvirus, zucchini yellow mosaic virus (zymv). in order to engineer melon lines resistant to zymv, a construct containing the translatable coat protein (cp) sequence coupled with the 3' non-translatable region of the virus was generated and used to transform an elite cultivar of oriental melon (silver light) mediated by agrobacterium using an improved cotyledon-cutting method. removal of 1-mm portion from the proximal end o ... | 2009 | 19479262 |
| hypersensitive response to aphis gossypii glover in melon genotypes carrying the vat gene. | aphis gossypii glover causes direct and indirect damage to cucumis melo l. crops. to decrease the harmful effects of this pest, one of the most economically and environmentally acceptable options is to use genetically resistant melon varieties. to date, several sources of resistance carrying the vat gene are used in melon breeding programmes that aim to prevent a. gossypii colonization and the subsequent aphid virus transmission. the results suggest that the resistance conferred by this gene is ... | 2009 | 19474089 |
| comparison of the efficacy of narrow band ultraviolet b and narrow band ultraviolet b plus topical catalase-superoxide dismutase treatment in vitiligo patients. | accumulation of free radicals in the epidermis and the role of oxidative stress have been demonstrated in the pathogenesis of vitiligo. so, new treatment modalities that support antioxidant systems may be a choice for treatment. we sought to determine the clinical efficacy of narrow band ultraviolet b plus topical formulation including cucumis melo superoxide dismutase and catalase (vitix), over the narrow band ultraviolet b treatment alone. thirty vitiligo patients (18 female, 12 male; mean age ... | 2016 | 19467974 |
| identification of host attractants for the ethiopian fruit fly, dacus ciliatus loew. | the ethiopian fruit fly, dacus ciliatus, is an oligophagous pest of cucurbit crops, particularly melons, cucumbers, and marrows (summer squash). the present study aimed to identify host attractants for d. ciliatus and was guided by a behavioral bioassay and an electrophysiological assay. we tested volatile compounds from the fruits of a host plant, ripe and unripe galia melon, cucumis melo var. reticulates. both sexes were attracted to melon volatiles. those of ripe melon were preferred. gas chr ... | 2009 | 19440796 |
| cloning and characterization of a sucrose synthase-encoding gene from muskmelon. | a full-length cdna clone encoding sucrose synthase (ss; ec 2.4.1.13) was isolated from muskmelon (cucumis melo l.) by rt-pcr and race. the clone, designated as cmss1, contains 2,585 nucleotides with an open reading frame of 2,412 nucleotides. the deduced 804 amino acid sequence showed high identities with other plant sucrose synthase. real time pcr analysis indicated that cmss1 expression differed among root, stem, leaf, flower and fruit tissues. the analysis during fruit development indicated t ... | 2010 | 19415524 |
| determining nitrogen requirements of aceros and buceros hornbills. | over 2 months, seven feeding trials were conducted at st. catherines island, ga, to quantify protein intake and utilization in captive mature nonreproducing aceros (n=3 spp.) and buceros (n=2 spp.) hornbills were fed homogeneous isocaloric diets. a mixture of bird of paradise pellets, grapes, and raisins was offered to the birds as grape-sized balls, supplemented with diced cantaloupe melon to maintain hydration. to vary the protein level (range 10.8-22.6%) within the diets, different amounts of ... | 2008 | 19360624 |
| cucurbitane-type triterpenoids from the stems of cucumis melo. | phytochemical investigation of the stems of cucumis melo led to the isolation and identification of 21 cucurbitane-type triterpenoids, including nine new compounds (1-9) and 12 known compounds. their structures were determined on the basis of spectroscopic analyses, chemical methods, and comparison with spectroscopic data in the literature. two known compounds, cucurbitacin b (10) and cucurbitacin a (11), showed significant cytotoxic activity against the proliferation of a549/atcc and bel7402 ce ... | 2009 | 19348465 |
| virus-specific read-through codon preference affects infectivity of chimeric cucumber green mottle mosaic viruses displaying a dengue virus epitope. | a cucumber green mottle mosaic virus (cgmmv) was used to present a truncated dengue virus type 2 envelope (e) protein binding region from amino acids 379 to 423 (eb4). the eb4 gene was inserted at the terminal end of the cgmmv coat protein (cp) open reading frame (orf). read-through sequences of tmv or cgmmv, caa-uag-caa-uua, or aaa-uag-caa-uua were, respectively, inserted in between the cp and the eb4 genes. the chimeric clones, prt, prg, and pcg+fsrtre, were transcribed into full-length capped ... | 2009 | 19325913 |
| the cucurbitaceae and solanaceae illustrated in medieval manuscripts known as the tacuinum sanitatis. | beginning in the last two decades of the 14th century, richly illuminated versions of the tacuinum sanitatis, the latin translation of an 11th-century arabic manuscript known as taqwim al-sihha bi al-ashab al-sitta, were produced in northern italy. these illustrated manuscripts provide a window on late medieval life in that region by containing some 200 full-page illustrations, many of which vividly depict the harvest of vegetables, fruits, flowers, grains, aromatics and medicinal plants. our ob ... | 2009 | 19318382 |
| pathogenicity and histopathology of rotylenchulus reniformis infecting cantaloup. | rotylenchulus reniformis was pathogenic to cantaloup (cucumis melo 'perlita') under greenhouse conditions. these findings confirm field symptoms of cantaloup infected with r. reniformis. histopathological studies show that the nematode penetrates the cortex perpendicular to the vascular system and comes to rest with the head against the endodermis in young roots. feeding stimulated the pericycle to either side of the endodermal feeding cell and caused cell hypertrophy with enlargement of the nuc ... | 1975 | 19308147 |
| phytoparasitic nematodes adjacent to established strawberry plantations. | plant-nematode populations associated with uncultivated vegetation, adjacent strawberry plants, and alternate crop sites were studied at three locations in minnesota. at one site (forest lake), paratylenchus projectus, meloidogyne hapla, and pratylenchus tenuis were frequently associated with the roots of native vegetation. these nematode species were also present in adjacent strawberry beds. among alternate crops observed, oats and muskmelon usually supported the fewest nematodes although moder ... | 1978 | 19305841 |
| 1h nmr, gc-ei-tofms, and data set correlation for fruit metabolomics: application to spatial metabolite analysis in melon. | a metabolomics approach combining (1)h nmr and gas chromatography-electrospray ionization time-of-flight mass spectrometry (gc-ei-tofms) profiling was employed to characterize melon (cucumis melo l.) fruit. in a first step, quantitative (1)h nmr of polar extracts and principal component analyses (pca) of the corresponding data highlighted the major metabolites in fruit flesh, including sugars, organic acids, and amino acids. in a second step, the spatial localization of metabolites was investiga ... | 2009 | 19298059 |
| effect of gamma rays on different explants of callus treatment of multiple shoots in cucumis melo cv. bathasa. | a mutagenesis programme was carried out using physical mutagens (gamma rays) on cucumis melo cv. bathasa. in irradiated seeds the number of shoots formed in the lower doses was significantly higher than the controls. decrease in the number of shoots and shoot bud formation was observed with higher doses in all the explants studies. there was complete lethality in the 10, 15 and 20 kr doses. in irradiated stem cultures the maximum number of shoots were observed in 2 kr. the number of shoots decre ... | 2008 | 19295084 |
| effect of temperature on infection and survival of rotylenchulus reniformis. | from infestation of lettuce with preinfective females to egg deposition, populations of rotylenchulus reniformis from baton rouge, louisiana; lubbock and weslaco, texas; and mayaguez, puerto rico, required 41, 13, 7, and 7 days at 15, 20, 25, and 34 c, respectively. no nematode infection occurred at 10 c with any r. reniformis population, and the population from puerto rico did not reproduce at 15 c. nematode survival was not influenced by temperature, since populations from texas and louisiana ... | 1988 | 19290223 |
| influence of glomus intraradices and soil phosphorus on meloidogyne incognita infecting cucumis melo. | the interaction among glomus intraradices, meloidogyne incognita, and cantaloupe was studied at three soil phosphorus (p) levels in a greenhouse. all plants grew poorly in soil not amended with p, regardless of mycorrhizal or nematode status. in soil amended with 50 mug p /g soil, m. incognita suppressed the growth of nonmycorrhizal plants by 84%. in contrast, growth of mycorrhizal plants inoculated with m. incognita was retarded by only 21%. a similar trend occurred in plants grown in soil with ... | 1989 | 19287577 |
| possible amelioration of atherogenic diet induced dyslipidemia, hypothyroidism and hyperglycemia by the peel extracts of mangifera indica, cucumis melo and citrullus vulgaris fruits in rats. | hitherto unknown efficacy of the peel extracts of mangifera indica (mi), cucumis melo (cm) and citrullus vulgaris (cv) fruits in ameliorating the diet-induced alterations in dyslipidemia, thyroid dysfunction and diabetes mellitus have been investigated in rats. in one study, out of 4 different doses (50-300 mg/kg), 200 mg/kg of mi and 100 mg/kg for other two peel extracts could inhibit lipidperoxidation (lpo) maximally in liver. in the second experiment rats were maintained on pre-standardized a ... | 2008 | 19276533 |
| relationship between crop losses and initial population densities of meloidogyne arenaria in winter-grown oriental melon in korea. | to determine the economic threshold level, oriental melon (cucumis melo l. cv. geumssaragi-euncheon) grafted on shintozoa (cucurbita maxima x cu. moschata) was planted in plots (2 x 3 m) under a plastic film in february with a range of initial population densities (pi) of meloidogyne arenaria. the relationships of early, late, and total yield to pi measured in september and january were adequately described by both linear regression and the seinhorst damage model. initial nematode densities in s ... | 2002 | 19265907 |
| effects of application strategies of fumigant and nonfumigant nematicides on cantaloupe grown in deep sand soils in florida. | a 2-year study was conducted in which three treatment tactics of oxamyl (at planting application, application every 2 weeks, and rescue applications, as determined by crop symptoms) were compared to fumigant treatments with methyl bromide, 1,3-dichloropropene (1,3-d), and 1,3-d plus chloropicrin for management of meloidogyne spp. in 2002, treatments that included 1,3-d produced higher yields as determined both by number and weight of marketable fruit. all treatment tactics relying solely on oxam ... | 2005 | 19262874 |
| use of cucumis metuliferus as a rootstock for melon to manage meloidogyne incognita. | root-knot nematode-susceptible melons (cantaloupe) were grown in pots with varying levels of meloidogyne incognita and were compared to susceptible melons that were grafted onto cucumis metuliferus or cucurbita moschata rootstocks. in addition, the effect of using melons as transplants in nematode-infested soil was compared to direct seeding of melons in nematode-infested soil. there were no differences in shoot or root weight, or severity of root galling between transplanted and direct-seeded n ... | 2005 | 19262873 |
| cadmium accumulation by muskmelon under salt stress in contaminated organic soil. | human-induced salinization and trace element contamination are widespread and increasing rapidly, but their interactions and environmental consequences are poorly understood. phytoaccumulation, as the crucial entry pathway for biotoxic cd into the human foodstuffs, correlates positively with rhizosphere salinity. hypothesising that organic matter decreases the bioavailable cd(2+) pool and therefore restricts its phytoextraction, we assessed the effects of four salinity levels (0, 20, 40 and 60 m ... | 2009 | 19162301 |
| [bee (apis mellifera, hymenoptera: apoidea) visitation to cantaloupe cucumis melo (cucurvitaceae) flowers in panama]. | flower visits by bees were observed in a melon cultivated field of san lorenzo district, in chiriqui, panama, from january 6 to february 19, 2002, from 6:30 am to 4:30 pm we recorded the duration of each foraging event and the type of resource collected. flower visits were mostly for nectar collection (approximately 75 %). pollen foraging was concentrated in the first hours of the morning and ended by 11:00 am the mean collection time was similar for both food resources, but was different betwee ... | 2007 | 19069779 |
| protective role of mangifera indica, cucumis melo and citrullus vulgaris peel extracts in chemically induced hypothyroidism. | an investigation was made to evaluate the pharmacological importance of fruit peel extracts of mangifera indica (mi), citrullus vulgaris (cv) and cucumis melo (cm) with respect to the possible regulation of tissue lipid peroxidation (lpo), thyroid dysfunctions, lipid and glucose metabolism. pre-standardized doses (200mg/kg of mi and 100mg/kg both of cv and cm), based on the maximum inhibition in hepatic lpo, were administered to wistar albino male rats for 10 consecutive days and the changes in ... | 2009 | 19059228 |
| curtovirus-cucurbit interaction: acquisition host plays a role in leafhopper transmission in a host-dependent manner. | curly top disease (ctd) of vegetable crops is caused by viruses in the genus curtovirus (family geminiviridae). cucurbits are reported to be susceptible to ctd; however, the disease is rare in california despite annual outbreaks in other hosts (e.g., common bean, pepper, sugar beet, and tomato). consistent with these observations, no obvious curly top symptoms were observed in melon fields surveyed for ctd in central california in 2004 and 2005, whereas the disease was readily observed in tomato ... | 2009 | 19055441 |
| alternative sanitizers to chlorine for use on fresh-cut "galia" (cucumis melo var. catalupensis) melon. | chlorine is commonly used to reduce microbial load in fresh-cut vegetables. however, the production of chlorinated organic compounds, such as trihalomethanes, which are potential carcinogens, has created the need to investigate the efficiency of nontraditional sanitizers and alternative techniques. the effects of 4 novel sanitizers were tested in fresh-cut "galia" melon: chlorine dioxide (clo(2)) at 3 mg/l, peracetic acid (paa) at 80 mg/l, hydrogen peroxide (h(2)o(2)) at 50 mg/l, and nisin at 25 ... | 2008 | 19021810 |
| pathogenic and genetic variation in the japanese strains of fusarium oxysporum f. sp. melonis. | abstract pathogenic variation among 41 japanese strains of fusarium oxysporum f. sp. melonis was analyzed by pathogenicity tests with muskmelon, oriental melon, and oriental pickling melon cultivars. based on pathogenicity to muskmelon cvs. amus and ohi and oriental melon cv. ogon 9, 41 strains were divided into 3 groups that corresponded completely to risser's races 0, 2, and 1,2y. to further characterize pathogenic variation within the forma specialis and races, strains were assayed for pathog ... | 1998 | 18944886 |
| the relationship of host range, physiology, and genotype to virulence on cantaloupe in pseudomonas syringae from cantaloupe blight epidemics in france. | abstract in 1993, a bacterial blight caused important losses of cantaloupe (cucumis melo var. cantalupensis) in southwestern france and has now been reported in all cantaloupe-growing regions of france. the causal agent of this blight is pseudomonas syringae, although on a worldwide basis this bacterium has not been a major pathogen of melon for over 50 years. to identify the pathovar of the cantaloupe pathogen, we employed biochemical tests, plasmid and chromosomal profiling, and host range stu ... | 2000 | 18944544 |
| induced systemic resistance and promotion of plant growth by bacillus spp. | abstract elicitation of induced systemic resistance (isr) by plant-associated bacteria was initially demonstrated using pseudomonas spp. and other gram-negative bacteria. several reviews have summarized various aspects of the large volume of literature on pseudomonas spp. as elicitors of isr. fewer published accounts of isr by bacillus spp. are available, and we review this literature for the first time. published results are summarized showing that specific strains of the species b. amyloliquef ... | 2004 | 18944464 |
| internal transcribed spacer regions 1 and 2 and random amplified polymorphic dna analysis of didymella bryoniae and related phoma species isolated from cucurbits. | abstract didymella bryoniae (anamorph phoma cucurbitacearum) is the causal agent of gummy stem blight, although other phoma species are often isolated from cucurbit plants exhibiting symptoms of the disease. the molecular and phylogenetic relationships between d. bryoniae and these phoma species are unknown. isolates of d. bryoniae and phoma obtained from cucurbits grown at various geographical locations in the united states were subjected to random amplified polymorphic dna (rapd) analysis and ... | 2002 | 18944025 |
| recovery from cucurbit leaf crumple virus (family geminiviridae, genus begomovirus) infection is an adaptive antiviral response associated with changes in viral small rnas. | a strong recovery response occurs in cantaloupe (cucumis melo) and watermelon (citrullus lanatus) infected with the bipartite begomovirus cucurbit leaf crumple virus (culcrv). this response is characterized by initially severe symptoms, which gradually become attenuated (almost symptomless). an inverse relationship was detected between viral dna levels and recovery, indicating that recovered tissues had reduced viral titers. recovered tissues also were resistant to reinfection with culcrv; i.e., ... | 2008 | 18943741 |
| a new strain of melon necrotic spot virus that is unable to systemically infect cucumis melo. | we report a new strain of melon necrotic spot virus (mnsv) that is unable to systemically infect cucumis melo. a spherical virus (w-isolate), about 30 nm in diameter like a carmovirus, was isolated from watermelons with necrotic symptoms. the w-isolate had little serological similarity to mnsv, and it did not cause any symptoms in six melon cultivars susceptible to mnsv; however, the host range of the w-isolate was limited exclusively to cucurbitaceous plants, and transmission by o. bornovanus w ... | 2008 | 18943404 |
| characterization of a regional population of fusarium oxysporum f. sp. niveum by race, cross pathogenicity, and vegetative compatibility. | abstract eighty-eight isolates of fusarium oxysporum f. sp. niveum, collected from wilted watermelon plants and infested soil in maryland and dela-ware, were characterized by cross pathogenicity to muskmelon, race, and vegetative compatibility. four isolates (4.5%) were moderately pathogenic to >/=2 of 18 muskmelon cultivars in a greenhouse test, and one representative isolate also was slightly pathogenic in field microplots. the four isolates all were designated as race 2, and were in vegetativ ... | 2007 | 18943287 |
| melon resistance to cucurbit yellow stunting disorder virus is characterized by reduced virus accumulation. | abstract the pattern of accumulation of cucurbit yellow stunting disorder virus (cysdv; genus crinivirus, family closteroviridae) rna has been analyzed in several cucurbit accessions. in susceptible accessions of melon (cucumis melo), cucumber (cucumis sativus), marrow (cucurbita maxima), and squash (cucurbita pepo), cysdv rna accumulation peaked during the first to second week postinoculation in the first to third leaf above the inoculated one; younger leaves showed very low or undetectable lev ... | 2003 | 18943165 |
| cerebroside elicitor confers resistance to fusarium disease in various plant species. | abstract in the rice blast fungus pathosystem, cerebroside, a compound categorized as a sphingolipid, was found in our previous study to be a non-racespecific elicitor, which elicits defense responses in rice. here we describe that cerebroside c is produced in diverse strains of fusarium oxysporum, a common soilborne agent of wilt disease affecting a wide range of plant species. in addition, some type of cerebroside elicitor involving cerebroside a, b, or c was detected in other soilborne phytop ... | 2004 | 18943100 |
| isolation of nonpathogenic mutants of fusarium oxysporum f. sp. melonis for biological control of fusarium wilt in cucurbits. | abstract two nonpathogenic mutant strains 4/4 and 15/15 of fusarium oxysporum f. sp. melonis (race 1,2) were isolated by a continuous dipinoculation technique following uv mutagenesis of the virulent wild-type isolate fom1.2. no disease symptoms or detrimental effects were observed following inoculation of muskmelon seedlings by strain 4/4. in contrast, strain 15/15 caused mortality of susceptible cultivars although to a lesser extent than the wild-type isolate. strain 4/4 colonized a variety of ... | 2002 | 18943089 |
| inheritance of resistance to watermelon mosaic virus in cucumis melo that impairs virus accumulation, symptom expression, and aphid transmission. | abstract the cucumis melo accession tgr-1551 was found to be resistant to watermelon mosaic virus (wmv, genus potyvirus, family potyviridae). the resistance resulted in a drastic and significant reduction of virus titer and infected plants were asymptomatic or exhibited mild disease symptoms. the same gene or closely linked genes restricted virus accumulation and ameliorated symptom expression. no effect was observed on virus accumulation in inoculated leaves, which suggested that the initial ph ... | 2005 | 18943018 |
| a seed-borne virus of muskmelon. | 1947 | 18895500 | |
| interactions of bacillus spp. and plants--with special reference to induced systemic resistance (isr). | biological control of soil-borne pathogens comprises the decrease of inoculum or of the disease producing activity of a pathogen through one or more mechanisms. interest in biological control of soil-borne plant pathogens has increased considerably in the last few decades, because it may provide control of diseases that cannot or only partly be managed by other control strategies. recent advances in microbial and molecular techniques have significantly contributed to new insights in underlying m ... | 2009 | 18845426 |
| employment of pumpkin (cucumis melo) urease entrapped in alginate beads for quantitation of cadmium in aqueous media. | the potential of employment of free as well as alginate-immobilized urease for the quantitation of cadmium (cd(2+)) was explored. urease from the seeds of pumpkin (cucumis melo) was purified to apparent homogeneity by heat treatment at 48 +/- 0.1 degrees c and gel filtration through sephadex g-200. the purified enzyme exhibited a single band on native page under coomassie brilliant blue and silver staining. the enzyme entrapped in 3.5% alginate beads (with 86% immobilization) exhibited no leachi ... | 2008 | 18830881 |
| on the genetic control of heterosis for fruit shape in melon (cucumis melo l.). | the objective of the present work is to study the genetic basis of heterosis for fruit shape (fs) in melon observed in a cross between the spanish cultivar "piel de sapo" (ps) and the korean accession pi 161375 (songwang charmi [sc]) using a set of near-isogenic lines (nils) with contrasting phenotypes for fs, each carrying a single chromosomal introgression from sc within the genetic background of ps. we investigated the fs of homozygous nils, hybrids nil x ps, and all 2-way crosses between nil ... | 2009 | 18815117 |
| bin mapping of genomic and est-derived ssrs in melon (cucumis melo l.). | we report the development of 158 primer pairs flanking ssr motifs in genomic (gssr) and est (est-ssr) melon sequences, all yielding polymorphic bands in melon germplasm, except one that was polymorphic only in cucurbita species. a similar polymorphism level was found among est-ssrs and gssrs, between dimeric and trimeric est-ssrs, and between est-ssrs placed in the open reading frame or any of the 5'- or 3'-untranslated regions. correlation between ssr length and polymorphism was only found for ... | 2008 | 18806992 |
| identification of qtls for resistance to powdery mildew and ssr markers diagnostic for powdery mildew resistance genes in melon (cucumis melo l.). | powdery mildew caused by podosphaera xanthii is an important foliar disease in melon. to find molecular markers for marker-assisted selection, we constructed a genetic linkage map of melon based on a population of 93 recombinant inbred lines derived from crosses between highly resistant ar 5 and susceptible 'earl's favourite (harukei 3)'. the map spans 877 cm and consists of 167 markers, comprising 157 simple sequence repeats (ssrs), 7 sequence characterized amplified region/cleavage amplified p ... | 2008 | 18797839 |
| mapping of genetic loci that regulate quantity of beta-carotene in fruit of us western shipping melon (cucumis melo l.). | melon (cucumis melo l.) is highly nutritious vegetable species and an important source of beta-carotene (vitamin a), which is an important nutrient in the human diet. a previously developed set of 81 recombinant inbred lines (ril) derived from group cantalupensis us western shipper market type germplasm was examined in two locations [wisconsin (wi) and california (ca), usa] over 2 years to identify quantitative trait loci (qtl) associated with quantity of beta-carotene (qbetac) in mature fruit. ... | 2008 | 18773190 |
| molecular cloning, characterization and expression analysis of cmapx. | the rt pcr and race methods were used to obtain the cdna sequence of an apx gene of muskmelon after the leaves were induced with powdery mildew. the cdna length of the apx gene is 1,047 bp with a 750 bp orf encoded a 249 amino acid and the molecular weight of apx protein is 27.3 kda. the analysis showed that the cmapx genomic dna contained 10 extrons and 9 introns. the identity of the amino acid sequence deduced from the cdna with the apx family of other homologous members was about 74-97%. a fu ... | 2009 | 18756374 |
| distribution and pathway for phloem-dependent movement of melon necrotic spot virus in melon plants. | the translocation of melon necrotic spot virus (mnsv) within tissues of inoculated and systemically infected cucumis melo l. 'galia' was studied by tissue-printing and in situ hybridization techniques. the results were compatible with the phloem vascular components being used to spread mnsv systemically by the same assimilate transport route that runs from source to sink organs. virus rnas were shown to move from the inoculated cotyledon toward the hypocotyl and root system via the external phlo ... | 2008 | 18705860 |
| functional characterization of the arabidopsis atsuc2 sucrose/h+ symporter by tissue-specific complementation reveals an essential role in phloem loading but not in long-distance transport. | atsuc2 (at1g22710) encodes a phloem-localized sucrose (suc)/h(+) symporter necessary for efficient suc transport from source tissues to sink tissues in arabidopsis (arabidopsis thaliana). atsuc2 is highly expressed in the collection phloem of mature leaves, and its function in phloem loading is well established. atsuc2, however, is also expressed strongly in the transport phloem, where its role is more ambiguous, and it has been implicated in mediating both efflux and retrieval to and from flank ... | 2008 | 18650401 |
| antisense suppression of an acid invertase gene (mai1) in muskmelon alters plant growth and fruit development. | to unravel the roles of soluble acid invertase in muskmelon (cucumis melo l.), its activity in transgenic muskmelon plants was reduced by an antisense approach. for this purpose, a 1038 bp cdna fragment of muskmelon soluble acid invertase was expressed in antisense orientation behind the 35s promoter of the cauliflower mosaic virus. the phenotype of the antisense plants clearly differed from that of control plants. the transgenic plant leaves were markedly smaller, and the stems were obviously t ... | 2008 | 18641398 |
| salmonella litchfield outbreak associated with a hotel restaurant--atlantic city, new jersey, 2007. | on july 10, 2007, the pennsylvania department of health notified the new jersey department of health and senior services (njdhss) of three culture-confirmed cases of salmonella litchfield infection with matching pulsed-field gel electrophoresis (pfge) patterns. data from pulsenet, the national molecular subtyping network for foodborne disease surveillance, confirmed 11 cases (including the three from pennsylvania) of this rarely identified salmonella serotype in five states during a 5-week perio ... | 2008 | 18636066 |
| nutritional composition, microbiological and sensory properties of dried melon: a traditional turkmen product. | seven different cultivars (taze vaharman, gökjek, arap hatli, türkmen vaharmani, torli payendeke, yazki bişek, gizil gülabi) of honeydew melons (cucumis melo l.) from turkmenistan were processed to a dried traditional product known as 'kavun kaki' in order to evaluate the most suitable cultivar for drying process. the chemical composition (dry matter, crude oil, crude protein, crude fibre, energy, ash, water-soluble solids, total sugar, invert sugar, titratable acidity, ph and mineral contents) ... | 2009 | 18608557 |
| comparative histochemical analyses of oxidative burst and cell wall reinforcement in compatible and incompatible melon-powdery mildew (podosphaera fusca) interactions. | the spatial-temporal expression patterns of oxidative burst and cell wall reinforcement were analyzed in leaves of resistant and susceptible melon (cucumis melo l.) cultivars in response to podosphaera fusca (fr.) braun & shishkoff, the main causal agent of powdery mildew in cucurbits. extensive development of powdery mildew mycelia and a progressive increase in haustorial count were recorded in the susceptible cultivar after 4d, while in the resistant cultivar powdery mildew failed to grow and ... | 2008 | 18585824 |
| occurrence and distribution of ten viruses infecting cucurbit plants in guilan province, iran. | during the 2006 and 2007 growing seasons, a systematic survey was conducted in open-field of melon (cucumis melo l.), cucumber (c. sativus l.), squash (cucurbita sp.), and watermelon (citrulus lanatus l.) crops in 16 major cucurbit-growing areas of guilan province in iran. symptomatic leaf samples were collected and screened by double-antibody sandwich elisa (das-elisa) or rt-pcr to detect zucchini yellow mosaic virus (zymv), watermelon mosaic virus (wmv), cucurbit aphid-borne yellows virus (cab ... | 2008 | 18564898 |
| a multi-state outbreak of salmonella saintpaul in australia associated with cantaloupe consumption. | a multi-state outbreak of salmonella enterica serovar saintpaul infection occurred in australia during october 2006. a case-control study conducted in three affected jurisdictions, new south wales, victoria and australian capital territory, included 36 cases with the outbreak-specific strain of s. saintpaul identified by multiple locus variable-number tandem repeat analysis (mlva) in a faecal specimen and 106 controls. consumption of cantaloupe (rockmelon) was strongly associated with illness (a ... | 2009 | 18559128 |
| survival of sa11 rotavirus in fresh fruit juices of pineapple, papaya, and honeydew melon. | survival of rotavirus in fresh fruit juices of papaya (caraca papaya l.), honeydew melon (cucumis melo l.), and pineapple (ananas comosus [l.] merr.) was studied. clarified juices were prepared from pulps of ripe fruits and sterilized by ultrafiltration. one milliliter of juice from each fruit was inoculated with 20 microl of 1 x 10(6) pfu of sa11 rotavirus and sampled immediately (0-h exposure) and 1 and 3 h later at 28 degrees c. mean viral titers in juices of papaya (ph 5.1) and honeydew melo ... | 2008 | 18522042 |
| molecular authentication of three italian melon accessions by arms-pcr and its1 (internal transcribed spacer 1) secondary structure prediction. | genetic assessment was carried out on three italian melon accessions by sequence and structural analysis of the internal transcribed spacer 1 (its1) from three populations belonging to two cucumis melo l. varieties (madras and tendral). alignment of the 18s-5.8s-26s sequences from three melon accessions showed that there were three single-nucleotide polymorphisms (snps) and one short insertion-deletion (indel) at the 5'end its1. an amplification refractory mutation system (arms)-pcr-based analys ... | 2008 | 18478086 |
| reduction by gaseous ozone of salmonella and microbial flora associated with fresh-cut cantaloupe. | this research investigates the efficacy of gaseous ozone, applied under partial vacuum in a controlled reaction chamber, for the elimination of salmonella inoculated on melon rind. the performance of high dose, short duration treatment with gaseous ozone, in this pilot system, on the microbial and sensory quality of fresh-cut cantaloupes was also evaluated. gaseous ozone (10,000 ppm for 30 min under vacuum) reduced viable, recoverable salmonella from inoculated physiologically mature non-ripe an ... | 2008 | 18456110 |
| antioxidant, sugar, mineral, and phytonutrient concentrations across edible fruit tissues of orange-fleshed honeydew melon (cucumis melo l.). | orange-fleshed, non-netted honeydew ( cucumis melo l.) is a relatively new melon in the marketplace and has shown a lot of potential as an alternative to netted muskmelons (cantaloupes), which are often prone to surface contamination by enteric bacteria. orange-fleshed honeydew is a cross between orange-fleshed cantaloupe and non-netted, green-fleshed honeydew. this glasshouse study investigated the nutritional profile (phytonutrient and sugar contents) in different tissues of mature orange-fles ... | 2008 | 18454549 |
| 3-methylthiopropionic acid ethyl ester, isolated from katsura-uri (japanese pickling melon, cucumis melo var. conomon), enhanced differentiation in human colon cancer cells. | the fully ripened fruit of katsura-uri japanese pickling melon ( cucumis melo var. conomon) has rarely been used for food because the midripened fruit is utilized for making pickles, but the fully ripened fruit is no longer valuable for pickles due to the fruit body being too soft. we have considered the utilization of the fully ripened katsura-uri fruit that may be used for nonpickling products, particularly if the fully ripened fruit demonstrated health benefits such as anticarcinogenic proper ... | 2008 | 18426216 |
| factorial design for the optimization of enzymatic detection of cadmium in aqueous solution using immobilized urease from vegetable waste. | free as well as alginate immobilized urease was utilized for detection and quantitation of cadmium (cd2+) in aqueous samples. urease from the seeds of pumpkin (cucumis melo), being a vegetable waste, was extracted and purified to apparent homogeneity (sp. activity 353 u/mg protein; a280/a260=1.12) by heat treatment at 48+/-0.1 degrees c and gel filtration through sephadex g-200. the homogeneous enzyme preparation was immobilized in 3.5% alginate leading to 86% immobilization and no leaching of t ... | 2008 | 18378444 |
| [effects of air temperature, solar radiation and soil water on dry matter accumulation and allocation of greenhouse muskmelon seedlings and related simulation models]. | with different sowing dates and irrigation upper limits, the effects of air temperature, solar radiation and soil water on the dry matter accumulation and allocation of greenhouse muskmelon seedlings were studied, with related simulation models established. the results showed that the dry matter accumulation and allocation of the seedlings had correlations with the changes of effective accumulative temperature, accumulative solar radiation, and irrigation upper limits at different seasons in a y ... | 2007 | 18333445 |
| ethylene production in plants during transformation suppresses vir gene expression in agrobacterium tumefaciens. | ethylene evolution from plants inhibits agrobacterium-mediated genetic transformation, but the mechanism is little understood. in this study, the possible role of ethylene in agrobacterium-mediated genetic transformation was clarified. it was tested whether or not plant ethylene sensitivity affected genetic transformation; the sensitivity might regulate bacterial growth during co-cultivation and vir gene expression in agrobacterium tumefaciens. for these experiments, melon (cucumis melo) was use ... | 2008 | 18331427 |
| melon fruits: genetic diversity, physiology, and biotechnology features. | among cucurbitaceae, cucumis melo is one of the most important cultivated cucurbits. they are grown primarily for their fruit, which generally have a sweet aromatic flavor, with great diversity and size (50 g to 15 kg), flesh color (orange, green, white, and pink), rind color (green, yellow, white, orange, red, and gray), form (round, flat, and elongated), and dimension (4 to 200 cm). c. melo can be broken down into seven distinct types based on the previously discussed variations in the species ... | 2008 | 18322855 |
| cloning and characterization of a soluble acid invertase-encoding gene from muskmelon. | soluble acid invertase (s-aiv; ec 3.2.1.26) is thought to play a critical role in sucrose hydrolysis in muskmelon (cucumis melo l.) fruit. a full-length cdna clone encoding s-aiv was isolated from muskmelon by rt-pcr and race. the clone, designated as cms-aiv1, contains 2178 nucleotides with an open reading frame of 1908 nucleotides. the deduced 636 amino acid sequence showed high identities with other plant soluble acid invertases. northern blot analysis indicated that cms-aiv1 was expressed in ... | 2009 | 18317948 |
| construction of a fosmid library of cucumber (cucumis sativus) and comparative analyses of the eif4e and eif(iso)4e regions from cucumber and melon (cucumis melo). | a fosmid library of cucumber was synthesized as an unrestricted resource for researchers and used for comparative sequence analyses to assess synteny between the cucumber and melon genomes, both members of the genus cucumis and the two most economically important plants in the family cucurbitaceae. end sequencing of random fosmids produced over 680 kilobases of cucumber genomic sequence, of which 25% was similar to ribosomal dnas, 25% to satellite sequences, 20% to coding regions in other plants ... | 2008 | 18273646 |
| monitoring the incidence and causes of diseases potentially transmitted by food in australia: annual report of the ozfoodnet network, 2006. | in 2006, ozfoodnet sites reported 24,598 notifications of seven diseases or conditions that are commonly transmitted by food, representing an increase of 2.5% over the mean of the previous 5 years. the most frequently notified aetiological agents were campylobacter (15,492 notifications) and salmonella (8,331 notifications). salmonella notifications increased in 2006 by 5.2% when compared to historical reports. the most common salmonella serotype notified in australia during 2006 was salmonella ... | 2007 | 18268875 |
| the molecular and biochemical basis for varietal variation in sesquiterpene content in melon (cucumis melo l.) rinds. | a combined chemical, biochemical and molecular study was conducted to understand the differential accumulation of volatile sesquiterpenes in melon fruits. sesquiterpenes were present mainly in the rinds of climacteric varieties, and a great diversity in their composition was found among varieties. sesquiterpenes were generally absent in non-climacteric varieties. two climacteric melon varieties, the green-fleshed 'noy yizre'el', and the orange-fleshed 'dulce' were further examined. in 'noy yizre ... | 2008 | 18264780 |
| [effects of moisture and light intensity on ecophysiological characteristics of muskmelon seedlings]. | the muskmelon variety 'xiholuotuo' was used as a test material in order to study the combined effects of substrate moisture and light intensity on growth, development and ecophysiological characteristics of muskmelon seedings in greenhouse. the results showed that the different substrate moisture and light intensity remarkably influenced the growth and development of muskmelon seedlings. with reduction of substrate moisture, chl a, chl b and chl a + b increased, while relative water content (rwc ... | 2007 | 18260450 |
| complete nucleotide sequence of a new isolate of tomato leaf curl new delhi virus infecting cucumber, bottle gourd and muskmelon in thailand. | 2008 | 18193155 | |
| edible alginate-based coating as carrier of antimicrobials to improve shelf-life and safety of fresh-cut melon. | the effect of malic acid and essential oils (eos) of cinnamon, palmarosa and lemongrass and their main active compounds as natural antimicrobial substances incorporated into an alginate-based edible coating on the shelf-life and safety of fresh-cut "piel de sapo" melon (cucumis melo l.) was investigated. melon pieces (50 g) were coated with alginate-based edible coating containing malic acid (ec) and eos or their active compounds before to be packed in air filled polypropylene trays and stored a ... | 2008 | 18164505 |
| dielectric properties of honeydew melons and correlation with quality. | dielectric properties of three honeydew melon cultivars, grown and harvested to provide a range of maturities, were measured with an open-ended coaxial-line probe and impedance analyzer over the frequency range from 10 mhz to 1.8 ghz. probe measurements were made on the external surface of the melons and also on tissue samples from the edible internal tissue. moisture content and soluble solids content (ssc) were measured for internal tissue samples, and ssc (sweetness) was used as the quality f ... | 2007 | 18161422 |
| effect of freezing and freezer storage on the ascorbic acid content of muskmelon, grapefruit sections, and strawberry purée. | 2016 | 18150591 | |
| effect of hand wash agents on controlling the transmission of pathogenic bacteria from hands to food. | the goals of this study were to evaluate the effectiveness of two hand wash regimens in reducing transient bacteria on the skin following a single hand wash and the subsequent transfer of the bacteria to a ready-to-eat food item, freshly cut cantaloupe melon. the number of bacteria recovered from hands and the quantity transferred to the melon were significantly less following the use of an antibacterial soap compared with plain soap. the antimicrobial soap achieved > 3-log reductions versus esc ... | 2007 | 18095447 |
| microbial survey of selected ontario-grown fresh fruits and vegetables. | recent produce-related outbreaks have been receiving heightened media coverage, which has increased public concern toward the safety of fresh fruits and vegetables. in response, the microbial contamination of ontario-grown fresh fruits and vegetables was evaluated by the ontario ministry of agriculture, food and rural affairs during the summer of 2004. prior to this survey, information specific to the microbial contamination of ontario-produced fruits and vegetables was limited. this nonregulato ... | 2007 | 18095445 |
| characterization and role of polyphenol oxidase and peroxidase in browning of fresh-cut melon. | polyphenol oxidase (ppo) and peroxidase (pod) were extracted from two different varieties of melon ( cucumis melo l. cantalupensis cv. charentais and c. melo l. inodorus cv. amarillo) and characterized using reliable spectrophotometric methods. in both cases the enzymes followed michaelis-menten kinetics, showing different values of kinetics parameters between the two cultivars: k m = 7.18 +/- 0.70 mm ('charentais') and 6.66 +/- 0.20 mm ('amarillo') mm; v max = 7.93 +/- 0.35 units/min ('charenta ... | 2008 | 18069784 |
| reme1, a copia retrotransposon in melon, is transcriptionally induced by uv light. | for the first time, numerous sequences of copia and gypsy retrotransposons from the cucumis melo genome have been obtained and analyzed. phylogenetic analyses of sequences of both types of long terminal repeat (ltr) retrotransposons were carried out. the melon genome contains approximately 20,000 gypsy and 6,800 copia elements, comprising about 26% of its total size. starting from a retrotransposon fragment, we have cloned and characterized an entire melon retrotransposon, named reme1, which is ... | 2008 | 18034313 |
| effect of gaseous ozone and hot water on microbial and sensory quality of cantaloupe and potential transference of escherichia coli o157:h7 during cutting. | the effect of gaseous ozone and hot water, alone or in combination, on the sensory and microbial quality of cantaloupe melon was investigated. escherichia coli o157:h7 transmission from the rind to edible melon flesh during cutting practices was also investigated. four different treatments consisting of hot water (75 degrees c, 1min), gaseous ozone (10,000ppm, 30min), gaseous ozone supplied by carbon monoxide gas and the combination of hot water and gaseous ozone were evaluated. sensory quality ... | 2008 | 17993390 |
| induction of a small heat shock protein and its functional roles in nicotiana plants in the defense response against ralstonia solanacearum. | in tobacco (nicotiana tabacum), ralstonia solanacearum oe1-1 (rsoe1-1) is pathogenic, whereas r. solanacearum 8107 (rs8107) is nonpathogenic and induces the hypersensitive response (hr). to elucidate the molecular mechanisms of plant-r. solanacearum interactions, we used differential display to isolate a cdna fragment, a6, regulated in tobacco by inoculation with rsoe1-1. the deduced amino acid sequence predicted from full-length a6-cdna showed similarity to small heat shock proteins from arabid ... | 2007 | 17965181 |
| correlating volatile compounds, sensory attributes, and quality parameters in stored fresh-cut cantaloupe. | changes in post-cutting volatiles, quality, and sensory attributes during fresh-cut storage (4 degrees c) of cantaloupe (cucumis melo l. var. reticulatus, naudin, cv. 'sol real') harvested at four distinct maturities (1/4-, 1/2-, 3/4-, and full-slip) were investigated after 0, 2, 5, 7, 9, 12, and 14 days in a 2-year study. increased fruity and sweet taste attributes were negatively correlated with percent acetates, aromatic acetates, and total aromatic compounds, and positively correlated with p ... | 2007 | 17944532 |
| enzymatic detection of mercuric ions in ground-water from vegetable wastes by immobilizing pumpkin (cucumis melo) urease in calcium alginate beads. | present report describes a quick and simple test based on enzyme inhibition for the detection of mercury in aqueous medium by urease immobilized in alginate beads. urease was extracted from the discarded seeds of pumpkin (cucumis melo) and was purified to apparent homogeneity (5.2-fold) by heat treatment at 48+/-0.1 degrees c and gel filtration through sephadex g-200. the homogeneous enzyme preparation (sp activity 353 u/mg protein, a(280)/a(260)=1.12) was immobilized in 3.5% alginate leading to ... | 2008 | 17933526 |
| the cucurbits of mediterranean antiquity: identification of taxa from ancient images and descriptions. | a critical analysis was made of cucurbit descriptions in dioscorides' de materia medica, columella's de re rustica and pliny's historia naturalis, works on medicine, agriculture and natural science of the 1st century ce, as well as the mishna and tosefta, compilations of rabbinic law derived from the same time period together with cucurbit images dating from antiquity including paintings, mosaics and sculpture. the goal was to identify taxonomically the mediterranean cucurbits at the time of the ... | 2007 | 17932073 |
| herbal medicines as diuretics: a review of the scientific evidence. | there is increasing interest in the health and wellness benefits of herbs and botanicals. this is with good reason as they might offer a natural safeguard against the development of certain conditions and be a putative treatment for some diseases. one such area may be the lowering of blood pressure in those where it is elevated (i.e., hypertension). one class of clinical medicines used to lower blood pressure are known as diuretics and work by increasing the excretion of urine from the body as w ... | 2007 | 17804183 |
| longevity of fusarium oxysporum in soil tube culture. | in soil tube culture, representatives of three biologic forms of fusarium oxysporum survived unchanged morphologically for 11 years or more. an isolate of the muskmelon wilt fungus remained viable after 17 years' storage in dry air at a temperature of from 3 degrees to 4 degrees c. the surviving unit was found to be the chlamydospore. | 1961 | 17800128 |
| melogen: an est database for melon functional genomics. | melon (cucumis melo l.) is one of the most important fleshy fruits for fresh consumption. despite this, few genomic resources exist for this species. to facilitate the discovery of genes involved in essential traits, such as fruit development, fruit maturation and disease resistance, and to speed up the process of breeding new and better adapted melon varieties, we have produced a large collection of expressed sequence tags (ests) from eight normalized cdna libraries from different tissues in di ... | 2007 | 17767721 |
| [effects of potassium level on photosynthetic characteristics and chloroplast submicroscopic structure of muskmelon leaves]. | with soilless culture in greenhouse, this paper studied the effects of three potassium (k) levels (120, 240, and 360 mg x l(-1)) on the photosynthetic characteristics and chloroplast submicroscopic structure of muskmelon cv. 'tiantian no. 1' leaves. the results showed that at low (120 mg x l(-1)) and high (360 mg x l(-1)) k levels, the net photosynthetic rate (pn) decreased, chloroplast lamellar structure disordered, and lamella number decreased. no significant effects were observed on co2 compe ... | 2007 | 17650859 |
| an alternative sampling technique for cucumber beetles (coleoptera: chrysomelidae) and diurnal beetle activity on muskmelon. | field studies were conducted to investigate the effectiveness of yellow sticky traps as an alternative sampling technique for striped, acalymma vittatum (f.), and spotted, diabrotica undecimpunctata howardi barber, cucumber beetles (coleoptera: chrysomelidae) and the diurnal beetle activity on muskmelon, cucumis melo l., near vincennes, in, in 2003 and 2004. the experimental design included six replications of seven 20-m-long rows each of muskmelon with 1.5 m between rows. on each sampling date, ... | 2007 | 17598544 |
| phylogenetics of cucumis (cucurbitaceae): cucumber (c. sativus) belongs in an asian/australian clade far from melon (c. melo). | melon, cucumis melo, and cucumber, c. sativus, are among the most widely cultivated crops worldwide. cucumis, as traditionally conceived, is geographically centered in africa, with c. sativus and c. hystrix thought to be the only cucumis species in asia. this taxonomy forms the basis for all ongoing cucumis breeding and genomics efforts. we tested relationships among cucumis and related genera based on dna sequences from chloroplast gene, intron, and spacer regions (rbcl, matk, rpl20-rps12, trnl ... | 2007 | 17425784 |
| one-step purification of histidine-tagged profilin with high purity and yield by using metal precipitation. | a simple one-step method for the purification of recombinant his-tagged profilin from the bacterial cell lysate is reported. noting the greater ease with which hexahistidine-tagged proteins can be metalprecipitated compared with unwanted protein impurities, we investigated the effect of lysis-buffer additives and optimization of other conditions to recover selectively desired proteins in a one-step metal precipitation without using biopolymers. purification of the his-tagged melon (cucumis melo) ... | 2007 | 17313372 |
| ethylene regulation of fruit softening and cell wall disassembly in charentais melon. | cell wall disassembly in ripening fruit is highly complex, involving the dismantling of multiple polysaccharide networks by diverse families of wall-modifying proteins. while it has been reported in several species that multiple members of each such family are expressed in the same fruit tissue, it is not clear whether this reflects functional redundancy, with protein isozymes from a single enzyme class performing similar roles and contributing equally to wall degradation, or whether they have d ... | 2007 | 17308329 |
| detection of qtl for yield-related traits using recombinant inbred lines derived from exotic and elite us western shipping melon germplasm. | the inheritance of yield-related traits in melon (cucumis melo l.; 2n = 2x = 24) is poorly understood, and the mapping of quantitative trait loci (qtl) for such traits has not been reported. therefore, a set of 81 recombinant inbred lines (ril) was developed from a cross between the monoecious, highly branched line usda 846-1 and a standard vining, andromonoecious cultivar, 'top mark'. the ril, parental lines, and three control cultivars ('esteem', 'sol dorado', and 'hales best jumbo') were grow ... | 2007 | 17294163 |
| effects of a cantaloupe melon extract/wheat gliadin biopolymer during aortic cross-clamping. | we previously reported in healthy volunteers that a cantaloupe melon extract chemically combined with wheat gliadin (melon extract/gliadin) and containing sod, catalase and residual glutathione peroxidase (gpx), protected against dna strand-break damage induced by hyperbaric oxygen (hbo), a well-established model of dna damage resulting from oxidative stress. aortic cross-clamping is a typical example of ischemia/reperfusion injury-related oxidative stress, and therefore we investigated whether ... | 2007 | 17237934 |
| effect of time before storage and storage temperature on survival of salmonella inoculated on fresh-cut melons. | the effects of a waiting period at room temperature ( approximately 22 degrees c) before refrigerating fresh-cut watermelon, cantaloupe and honeydew pieces contaminated with salmonella on survival of the inoculated pathogen were investigated. whole cantaloupes, honeydew melons and watermelons were washed with water, and fresh-cut pieces from individual melons were prepared and inoculated with a five strain cocktail of salmonella at 10(5)cfu/ml. populations of aerobic mesophilic bacteria, yeast a ... | 2007 | 17188207 |
| mutator-like elements identified in melon, arabidopsis and rice contain ulp1 protease domains. | the transposon mutator was first identified in maize, and is one of the most active mobile elements in plants. the arabidopsis thaliana genome contains at least 200 mutator-like elements (mules), which contain the mutator-like transposase gene, and often additional genes. we have detected a novel type of mules in melon (cumule), which, besides the transposase, contains two ubiquitin-like specific protease-like sequences (ulp1). this element is not present in the observed location in some melon c ... | 2007 | 17136348 |
| development of loop-mediated isothermal amplification (lamp)-based snp markers for shelf-life in melon (cucumis melo l.). | in this study, lamp markers linked to shelf-life in melon (cucumis melo l.) were developed by converting a cleaved amplified polymorphic sequences (caps) marker (c2). the caps-pcr fragments from the long-shelf-life melon (o-3) and short-shelf-life melon (nat-2) were cloned and sequenced to construct lamp primers. a single nucleotide polymorphism (snp) was identified between o-3 and nat-2. lamp primers were designed to detect the snp. in the lamp reaction to detect long-shelf-life melon, the turb ... | 2006 | 17132894 |
| the melon fruit fly, bactrocera cucurbitae: a review of its biology and management. | the melon fruit fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae) is distributed widely in temperate, tropical, and sub-tropical regions of the world. it has been reported to damage 81 host plants and is a major pest of cucurbitaceous vegetables, particularly the bitter gourd (momordica charantia), muskmelon (cucumis melo), snap melon (c. melo var. momordica), and snake gourd (trichosanthes anguina). the extent of losses vary between 30 to 100%, depending on the cucurbit species and ... | 2005 | 17119622 |
| hydroperoxide lyases (cyp74c and cyp74b) catalyze the homolytic isomerization of fatty acid hydroperoxides into hemiacetals. | the conversion of linoleic acid 9-hydroperoxide (9-hpod) by recombinant melon (cucumis melo l.) hydroperoxide lyase (hpl, cyp74c subfamily) was studied. short (5 s-1 min) incubations at 0 degrees c followed by rapid extraction and trimethylsilylation made it possible to trap a new unstable (t(1/2) <30 s) product, i.e. the hemiacetal (1'e,3'z)-9-hydroxy-9-(1',3'-nonadienyloxy)-nonanoic acid. identification was performed by gc-ms analysis and substantiated by the formation of trimethylsilyl 9-trim ... | 2006 | 17049304 |