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changes in polyamine content and localization of pinus sylvestris adc and suillus variegatus odc mrna transcripts during the formation of mycorrhizal interaction in an in vitro cultivation system.the involvement of polyamines (pas) in the interaction between pinus sylvestris l. seedlings and an ectomycorrhizal fungus suillus variegatus (swatz: fr.) o. kunze was studied in an in vitro cultivation system. pa concentrations in seedlings were analysed after 1, 3, and 5 weeks in dual culture with s. variegatus, and changes in pa pools were compared with the growth of the seedlings. pinus sylvestris arginine decarboxylase (adc) and s. variegatus ornithine decarboxylase (odc) mrna transcripts w ...200616868043
root penetration of sealing layers made of fly ash and sewage sludge.fly ash and sewage sludge are suggested materials for constructing sealing layers covering mine tailings impoundments. little is known, however, of their effect on vegetation or resistance to root penetration. we investigate: (i) the ability of different plant species to grow in sealing layers comprising fly ash and sewage sludge, (ii) the impact on plant growth of freshly hardened fly ash compared to aged and leached ash, and (iii) the plant stress response to fly ashes of different properties. ...200616825445
uptake, metabolism and distribution of organic and inorganic nitrogen sources by pinus sylvestris.although an increasing number of studies show that many plant species have the capacity to take up amino acids from exogenous sources, the importance of such uptake for plant nitrogen nutrition is largely unknown. moreover, little is known regarding metabolism and distribution of amino acid-n following uptake or of the regulation of these processes in response to plant nitrogen status. here results are presented from a study following uptake, metabolism, and distribution of nitrogen from no(3)(- ...200616820399
correlating gene expression to physiological parameters and environmental conditions during cold acclimation of pinus sylvestris, identification of molecular markers using cdna microarrays.scots pine (pinus sylvestris l.) seedlings were grown under different conditions (three field locations, two seasons and two climate room regimes), and then analyzed for freezing tolerance of shoots and roots and for transcript abundance in apical buds based on a cdna microarray containing about 1500 expressed sequence tags (ests) from buds of cold-treated scots pine seedlings. in a climate room providing long daily photoperiods and high temperatures, seedlings did not develop freezing tolerance ...200616815832
foliar application of ga3 during terminal long-shoot bud development stimulates shoot apical meristem activity in pinus sylvestris seedlings.the effect of exogenous gibberellin (ga3) on shoot apical meristem activity in conifer vegetative buds was investigated by spraying 0 or 0.1% ga3 on the foliage of first-year scots pine (pinus sylvestris l.) seedlings twice weekly for 9 weeks during development of the terminal long-shoot bud. exogenous ga3 promoted mitotic activity in the apical zone, thereby increasing both the rate and duration of cataphyll formation and giving rise to a higher and wider apical meristem. the increase in number ...200616815829
[age dynamics of genetic variation in an isolated population of chalk pine pinus sylvestris var. cretacea kalenicz. ex kom. from donbass].based on analysis of variation at ten allozyme loci in three age groups (25-35, 40-80, and more than 100 years of age) of plants and in seed embryos, demographic dynamics of the gene pools was studied in a small (60.5 ha) isolated relict population of chalk pine pinus sylvestris var. cretacea kalenicz. ex kom. from the steppe zone of ukraine. the observed grenotype proportions in these tree groups were shown to fit hardy-weinberg expectations, while in the embryos of their seeds, an excess of ho ...200616808246
spermidine and the ectomycorrhizal fungus pisolithus tinctorius synergistically induce maturation of scots pine embryogenic cultures.exogenous spermidine (spd) and the ectomycorrhizal (ecm) fungus pisolithus tinctorius (pers.) coker and couch had a synergistic effect on the maturation of scots pine (pinus sylvestris l.) somatic embryos. induced maturation was expressed as a higher number of cell masses able to form embryos and a greater number of embryos formed per cell mass. in contrast, treatment with p. tinctorius alone on the hormone-free medium resulted in the lowest embryo-forming capacity. retarded proliferation growth ...200716777261
relation between individual tree mortality and tree characteristics in a polluted and non-polluted environment.data on individual tree mortality in relatively healthy (berezinskiy biosphere reserve) and damaged (surroundings of the mineral fertilizer plant 'achema') even-aged scots pine (pinus sylvestris l.) stands are presented. tree size and competition intensity were found to be the most significant predictors of individual tree mortality in all age groups of the relatively healthy scots pine stands, however, an essential reduction in the closeness of relations between the tree mortality rate and thes ...200616738768
effect of organic amendment and plant roots on the solubility and mobilization of lead in soils at a shooting range.lead (pb) dissolving gradually from spent pellets constitutes a serious environmental risk in and near shooting ranges, and remediation measures are necessary to prevent its movement to deeper soil layers and ground water. in this study, the effectiveness of organic amendment and plant roots in stabilizing pb was assessed in a microcosm experiment. planted (scots pine, pinus sylvestris l.) and unplanted microcosms consisting of coarse-textured mineral soil covered with pb-contaminated humic tops ...200616738387
effects of heavy metal pollution on genetic variation and cytological disturbances in the pinus sylvestris l. population.this isoenzymatic and cytogenetic study has shown significant differences in genetic composition between two groups of pinus sylvestris trees: tolerant and sensitive to heavy metal pollution. total and mean numbers of alleles and genotypes per locus were higher in the pollution-sensitive group of trees, but heterozygosity (ho) was lower in this group. fixation index (f) indicates that trees tolerant for pollution were in the hardy-weinberg equilibrium, while the sensitive group had a significant ...200616682749
biochemical plant responses to ozone : ii. induction of stilbene biosynthesis in scots pine (pinus sylvestris l.) seedlings.formation of the stilbenes pinosylvin and pinosylvin 3-methyl ether, as well as the activity of the biosynthetic enzyme stilbene synthase (pinosylvin-forming), were induced several hundred- to thousandfold in primary needles of 6-week-old pine (pinus sylvestris l.) seedlings upon exposure to a single pulse of ozone of at least 0.15 microliters per liter. the seedlings required 4 hours of exposure as a minimum for the induction of stilbene biosynthesis when exposed to 0.2 microliters per liter oz ...199116668544
defense mechanisms of conifers : relationship of monoterpene cyclase activity to anatomical specialization and oleoresin monoterpene content.cell-free extracts from pinus ponderosa lawson (ponderosa pine) and pinus sylvestris l. (scotch pine) wood exhibited high levels of monoterpene synthase (cyclase) activity, whereas bark extracts of these species contained no detectable activity, and they inhibited cyclase activity when added to extracts from wood, unless polyvinylpyrrolidone was included in the preparation. the molecular mass of the polyvinylpyrrolidone added was of little consequence; however, polyvinylpolypyrrolidone (a cross- ...199116668182
isolation, purification, and subcellular localization of isozymes of superoxide dismutase from scots pine (pinus sylvestris l.) needles.two of four isozymes of superoxide dismutase (sod) (ec 1.15.1.1) were purified from scots pine (pinus sylvestris l.) needles. one form was cytosolic (sod-1) and the other was associated with chloroplasts (sod-3). the holoenzyme molecular masses was estimated at approximately 35 kilodaltons by gel filtration. the subunit molecular weight of the dimeric enzymes was estimated to 16.5 kilodaltons (sod-1) and 20.4 kilodaltons (sod-3) on sodium dodecyl sulfatepolyacrylamide gels. the nh(2)-terminal se ...199116667953
tracheid production in response to changes in the internal level of indole-3-acetic acid in 1-year-old shoots of scots pine.different concentrations of indole-3-acetic acid (iaa) were applied in lanolin to 1-year-old shoots of pinus sylvestris (l.) in a manner known to stimulate cambial activity. the internal concentration of free iaa was measured at a distance below the application point by combined gas chromatography-selected ion monitoring-mass spectrometry using [(13)c(6)]iaa as a quantitative internal standard, and related to the production of tracheids at the same site. the experiment was performed with: (a) de ...199016667908
distribution of indole-3-acetic acid and the occurrence of its alkali-labile conjugates in the extraxylary region of pinus sylvestris stems.free and conjugated indole-3-acetic acid (iaa) were measured by quantitative gas chromatography-selected ion monitoringmass spectrometry in the extraxylary region of the stem of large pinus sylvestris (l.) trees during the annual cycle of cambial activity and dormancy. the extraxylary region at the stem top and bottom was divided into 3 and 4 fractions, respectively, for the free iaa measurements, while the entire extraxylary region was extracted when the iaa-conjugates were analyzed. the effect ...199016667616
extended host range of agrobacterium tumefaciens in the genus pinus.two-to 4-month-old seedlings of nine pine species (pinus eldarica medw., pinus elliottii engelm., pinus jeffreyi grev. & balf., pinus lambertiana dougl., pinus ponderosa laws., pinus radiata d. don, pinus sylvestris l., pinus taeda l., pinus virginiana mill), douglas fir (pseudotsuaa menziesii (mirb.) franco) and incense cedar (libocedrus decurrens torr.) were inoculated with five strains of agrobacterium tumefaciens. transformation occurred in all conifer species tested as determined by gall fo ...199016667394
rapid effects of red light on the isopentenyladenosine content in scots pine seeds.red light (r) stimulates germination in scots pine seed (pinus sylvestris l.). the response is far red (fr) reversible. the dynamics of cytokinin changes following light treatment was investigated. extracts were purified by immunoaffinity and high performance liquid chromatography. n(6)-(delta(2)-isopentenyl) adenosine (ipa) and trans-zeatin riboside (zr) were quantified by both uv-absorbance of high performance liquid chromatography peaks and by enzyme-linked immunosorbent assay. identification ...198916667042
assay and electrophoresis of superoxide dismutase from red spruce (picea rubens sarg.), loblolly pine (pinus taeda l.), and scotch pine (pinus sylvestris l.) : a method for biomonitoring.this paper reports a method for extracting the antioxidant enzyme superoxide dismutase (sod) from the needles of red spruce (picea rubens sarg.), loblolly pine (pinus taeda l.), and scotch pine (pinus sylvestris l.) with high efficiency and free from interfering compounds. the extraction employs phosphate buffer with polyvinylpolypyrrolidone and triton x-100 followed by dialysis overnight. the isozymes of sod in each species were separated electrophoretically and tested for their sensitivity to ...198916666837
identification and quantification of indole-3-methanol in etiolated seedlings of scots pine (pinus sylvestris l.).combined gas chromatography-mass spectrometry has been used to identify indole-3-methanol in a purified buffer extract from etiolated seedlings of pinus sylvestris l. quantitative estimates obtained by high performance liquid chromatography with fluorescence detection, corrected for losses occurring during purification, indicated that etiolated seedlings of p. sylvestris contained 19.7 +/- 1.4 nanograms (+/- standard deviation) indole-3-methanol per gram fresh weight. the stability of indole-3-m ...198516664170
cold acclimation of suspension cultures of pinus sylvestris in response to light and temperature treatments.suspension cultures of pinus sylvestris l. (provenance södra ydre) were used to determine frost hardiness after manipulating daylengths and temperatures. frost hardiness was determined with the triphenyltetrazolium chloride (ttc) reduction method. the cultures were able to acclimate and increase frost hardiness levels; both low temperature (2 degrees c) and short day (8 h) treatments were used at the same time, but increased survival temperatures were not achieved when only one type of stimulus ...198316663151
influence of the irradiance on carbohydrate content and rooting of cuttings of pine seedlings (pinus sylvestris l.).seedlings of pinus sylvestris l. were grown for 6 weeks at an irradiance of either 8 or 40 watts per square meter in a controlled environment room. cuttings from these plants were rooted in tap water for 75 days at either 8 or 40 watts per square meter. the photoperiod was 17 hours.during the first 30 days of the rooting period quantitative changes in carbohydrates were recorded in cuttings from the different treatments. the carbohydrate contents of the cuttings were mainly regulated by the irra ...197816660437
searching for a new putative cryptic virus in pinus sylvestris l.double-stranded rnas (dsrnas) were detected in different pine populations in germany and hungary. two dsrna species of 1.5 and 1.58 kbp, respectively, persisted in the same trees for at least 2 years and their presence was not associated with any symptoms. the dsrnas were found to sediment in the vlp (virus-like particles) fraction and to be protected by protein(s) against rnase a digestion at low salt. cdna cloning and sequencing of the smaller segment (dsrna2) led to the identification of a pu ...200616604450
intra-annual radial growth and water relations of trees: implications towards a growth mechanism.there is a missing link between tree physiological and wood-anatomical knowledge which makes it impossible mechanistically to explain and predict the radial growth of individual trees from climate data. empirical data of microclimatic factors, intra-annual growth rates, and tree-specific ratios between actual and potential transpiration (t pet(-1)) of trees of three species (quercus pubescens, pinus sylvestris, and picea abies) at two dry sites in the central wallis, switzerland, were recorded f ...200616556628
comparison of reml and gibbs sampling estimates of multi-trait genetic parameters in scots pine.multi-trait (co)variance estimation is an important topic in plant and animal breeding. in this study we compare estimates obtained with restricted maximum likelihood (reml) and bayesian gibbs sampling of simulated data and of three traits (diameter, height and branch angle) from a 26-year-old partial diallel progeny test of scots pine (pinus sylvestris l.). based on the results from the simulated data we can conclude that the reml estimates are accurate but the mode of posterior distributions f ...200616544126
endobacteria in some ectomycorrhiza of scots pine (pinus sylvestris).the diversity of cultivable endobacteria associated with four different ectomycorrhizal morphotypes (suillus flavidus, suillus variegatus, russula paludosa and russula sp.) of scots pine (pinus sylvestris) was analysed by restriction fragment length polymorphism profiling of pcr-amplified rdna intergenic spacer regions and by sequence analyses of 16s rrna genes. ectomycorrhizal root tip surface-sterilization methods were developed and assessed for their efficiencies. bacterial communities from s ...200616542403
importance of mycorrhization helper bacteria cell density and metabolite localization for the pinus sylvestris-lactarius rufus symbiosis.mycorrhization helper bacteria, paenibacillus sp. ejp73 and burkholderia sp. ejp67, were used to study the importance of bacterial inoculum dose and bacterial derived soluble and volatile metabolites localization for enhancing mycorrhiza formation in the pinus sylvestris-lactarius rufus symbiosis, using a laboratory based microcosm. ejp73 and ejp67 produced different responses in relation to the inoculum dose; ejp73 significantly enhanced mycorrhiza formation to the same degree at all doses test ...200616542402
organic anion exudation by ectomycorrhizal fungi and pinus sylvestris in response to nutrient deficiencies.low molecular weight organic anions (lmwoa) can enhance weathering of mineral grains. we tested the hypothesis that ectomycorrhizal (ecm) fungi and tree seedlings increase their exudation of lmwoa when supply of magnesium, potassium and phosphorus is low to enhance the mobilization of mg, k and p from mineral grains. ectomycorrhizal fungi and pinus sylvestris seedlings were cultured in symbiosis and in isolation on glass beads with nutrient solution or with sand as a rooting medium, with a compl ...200616539612
long-term exposure to enhanced uv-b radiation has no significant effects on growth or secondary compounds of outdoor-grown scots pine and norway spruce seedlings.the effects of long-term enhanced uv-b radiation on growth and secondary compounds of two conifer species were studied in an outdoor experiment. scots pine (pinus sylvestris) seedlings were exposed for two growing seasons and norway spruce (picea abies) seedlings for three growing seasons to supplemental uv-b radiation, corresponding to a 30% increase in ambient uv-b radiation. the experiment also included appropriate controls for ambient and increased uv-a radiation. enhanced uv-b did not affec ...200616515828
lignans from the bark extract of pinus sylvestris l.three lignans were isolated from a bioactive fraction of the 70% aqueous acetone extract of the pine (pinus sylvestris l.) bark. their structures were identified by high-resolution positive ion ei mass spectrometry and nmr spectroscopy. the complete assignment of proton and carbon signals was achieved by 2d nmr experiments: hsqc, hmbc, dqf-cosy and noesy.200616489551
the role of below-ground competition during early stages of secondary succession: the case of 3-year-old scots pine (pinus sylvestris l.) seedlings in an abandoned grassland.in abandoned or extensively managed grasslands, the mechanisms involved in pioneer tree species success are not fully explained. resource competition among plants and microclimate modifications have been emphasised as possible mechanisms to explain variation of survivorship and growth. in this study, we evaluated a number of mechanisms that may lead to successful survival and growth of seedlings of a pioneer tree species (pinus sylvestris) in a grass-dominated grassland. three-year-old scots pin ...200616489460
qualitative and quantitative determination of extractives in heartwood of scots pine (pinus sylvestris l.) by gas chromatography.a method for quantitative determination of extractives from heartwood of scots pine (pinus sylvestris l.) using gas chromatography (gc) with flame ionization detection (fid) was developed. the limit of detection (lod) was 0.03 mg/g wood and the linear range (r = 0.9994) was up to 10 mg/g with accuracy within +/- 10% and precision of 18% relative standard deviation. the identification of the extractives was performed using gas chromatography combined with mass spectrometry (gc-ms). the yields of ...200616472534
high levels of asparagine synthetase in hypocotyls of pine seedlings suggest a role of the enzyme in re-allocation of seed-stored nitrogen.a pine asparagine synthetase gene expressed in developing seedlings has been identified by cloning its cdna (psas1) from scots pine (pinus sylvestris l.). genomic dna analysis with psas1 probes and a sequence-based phylogenetic tree are consistent with the possibility of more than one gene encoding asparagine synthetase in pine. however, the parallel patterns of free asparagine content and psas1 products indicate that the protein encoded by this gene is mainly responsible for the accumulation of ...200616425030
habitat factors associated with bank voles (clethrionomys glareolus) and concomitant hantavirus in northern sweden.puumala virus (puuv), genus hantavirus, causes nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome in humans. in this study, bank voles, the natural reservoir of puuv, were captured at locations of previous human puuv exposure and paired controls within a region of high incidence in northern sweden. the aim of the study was to evaluate the influence of environmental factors on the abundance of bank voles and the occurrence of puuv. the total number of voles and the numbe ...200516417427
oxalate and ferricrocin exudation by the extramatrical mycelium of an ectomycorrhizal fungus in symbiosis with pinus sylvestris.accurate estimates of mycelial exudation in time and space are crucial for the assessment of ectomycorrhizal involvement in biogeochemical processes. knowledge of exudation from mycelia of ectomycorrhizal fungi is still limited, especially for fungi in symbiosis with a host. pinus sylvestris seedlings colonized by hebeloma crustuliniforme were grown in aseptic multicompartment dishes. this novel system enabled identification of exudates originating only from extramatrical mycelium. at harvest, h ...200616411939
radial growth of pinus sylvestris growing on alluvial terraces is sensitive to water-level fluctuations.along the alpine river lech (tyrol, austria), poorly grown scots pine (pinus sylvestris) stands dominate on dry alluvial terraces, which are made up of coarse calcareous gravel. here we evaluated the impact of environmental factors, such as precipitation, temperature and water table, on annual variability of radial growth. tree-ring chronologies from six stands comprising different age classes were developed by extracting two core samples from more than 15 trees per plot. pearson correlations we ...200616411933
molecular characterization of a receptor-like protein kinase gene from pine (pinus sylvestris l.).we are developing molecular approaches to study the growth and development of woody plants. as part of our research efforts, we report the molecular cloning and characterization of psrlk here, a cdna from the conifer scots pine (pinus sylvestris l.) encoding a polypeptide similar to the receptor protein kinases described in angiosperms. a full-length clone was isolated from a cdna library constructed with poly (a)+ enriched rna prepared from germinating pine seeds. characterization of the isolat ...200616395588
estimation of wood density and chemical composition by means of diffuse reflectance mid-infrared fourier transform (drift-mir) spectroscopy.sitka spruce (picea sitchensis) samples (491) from 50 different clones as well as 24 different tropical hardwoods and 20 scots pine (pinus sylvestris) samples were used to construct diffuse reflectance mid-infrared fourier transform (drift-mir) based partial least squares (pls) calibrations on lignin, cellulose, and wood resin contents and densities. calibrations for density, lignin, and cellulose were established for all wood species combined into one data set as well as for the separate sitka ...200616390174
effects of four tanniferous plant extracts on the in vitro exsheathment of third-stage larvae of parasitic nematodes.the anthelmintic properties of tanniferous plants and of their secondary metabolites represent one possible alternative to chemotherapy that is currently being explored as a means of achieving sustainable control of gastrointestinal nematodes in ruminants. previous in vivo and in vitro results suggest that tanniferous plants can have direct anti-parasitic effect against different stages of nematodes. however, the mode of action of the bioactive plant compounds remains obscure. the objectives of ...200616388690
anatomical-based defense responses of scots pine (pinus sylvestris) stems to two fungal pathogens.we investigated the cellular responses of stem tissues of mature scots pine (pinus sylvestris l.) trees to inoculations with two fungal pathogens. the bark beetle vectored fungus, leptographium wingfieldii morelet, induced longer lesions in the bark, stronger swelling of polyphenolic parenchyma cells, more polyphenol accumulation and increased ray parenchyma activity compared with the root rot fungus, heterobasidion annosum (fr.) bref., or mechanical wounding. axial resin ducts in the xylem are ...200616356912
excitation energy partitioning and quenching during cold acclimation in scots pine.we studied the influence of two irradiances on cold acclimation and recovery of photosynthesis in scots pine (pinus sylvestris l.) seedlings to assess mechanisms for quenching the excess energy captured by the photosynthetic apparatus. a shift in temperature from 20 to 5 degrees c caused a greater decrease in photosynthetic activity, measured by chlorophyll fluorescence and oxygen evolution, in plants exposed to moderate light (350 micromol m(-2) s(-1)) than in shaded plants (50 micromol m(-2) s ...200616356904
[characteristics of gene expression in morphologically abnormal needles of scots pine].expression of 373 genes in scots pine morphologically abnormal needles has been analyzed with microarray analysis. it was found microarray technique allows detecting the minor changes in gene expression. the results obtained showed that changes in expression of 42 genes occur during the process of abnormal morphogenesis in the pinus sylvestris needles. the increasing of expression level of genes encoding ubiquitin-conjugating enzyme and l-ascorbate peroxidase was revealed for all samples analyze ...200516335273
phenology, natural enemies, and efficacy of horticultural oil for control of chionaspis heterophyllae (homoptera: diaspididae) on christmas tree plantations.pine needle scale, chionaspis pinifoliae (fitch), and chionaspis heterophyllae cooley are important pests of scots pine, pinus sylvestris l., and other conifers in much of north america. on christmas tree plantations, these insects are typically controlled by spraying broad-spectrum insecticides when the vulnerable immature stages are present. however, effective control of bivoltine populations can be difficult to achieve due to asynchronous hatch and development of the second generation. our ob ...200516334330
characterization of the photosynthetic apparatus in cortical bark chlorenchyma of scots pine.winter-induced inhibition of photosynthesis in scots pine (pinus sylvestris l.) needles is accompanied by a 65% reduction of the maximum photochemical efficiency of photosystem ii (psii), measured as fv/fm, but relatively stable photosystem i (psi) activity. in contrast, the photochemical efficiency of psii in bark chlorenchyma of scots pine twigs was shown to be well preserved, while psi capacity was severely decreased. low-temperature (77 k) chlorophyll fluorescence measurements also revealed ...200616333639
connections between climatic variables and the growth and needle dynamics of scots pine (pinus sylvestris l.) in estonia and lapland.the relationships between climatic variables and scots pine (pinus sylvestris l.) growth and needle dynamics were studied in three stands in estonia and in four stands located near the northern timberline in lapland. the trees sampled in estonia had low correlations with the analysed climatic variables (air temperature, precipitation and indices of atmospheric circulation). moreover, the weak cross-correlation of the time-series of the estonian sample trees indicated that scots pine is affected ...200616331502
presence of nitrosospira cluster 2 bacteria corresponds to n transformation rates in nine acid scots pine forest soils.the relation between environmental factors and the presence of ammonia-oxidising bacteria (aob), and its consequences for the n transformation rates were investigated in nine scots pine (pinus sylvestris l.) forest soils. in general, the diversity in aob appears to be strikingly low compared to other ecosystems. nitrosospira cluster 2, as determined by temporal temperature gradient electrophoresis and sequencing, was the only sequence cluster detected in the five soils with high nitrification ra ...200516329965
degradability of dissolved soil organic carbon and nitrogen in relation to tree species.the degradability and chemical characteristics of water-extractable dissolved organic carbon (doc) and nitrogen (don) from the humus layer of silver birch (betula pendula roth), norway spruce (picea abies (l.) karst.) and scots pine (pinus sylvestris l.) stands were compared in short-term incubation of soil solutions. for all extracts the degradation of doc and don was low (12-17% loss) and increased in the order: birch, spruce and pine. in the humus layer under pine a relatively larger pool of ...200516329927
the chemistry of soils, rocks and plant bioindicators in three ecosystems of the holy cross mountains, poland.in june of 2000, biogeochemical study was carried out in three ecosystems of the holy cross mountains (south-central poland). this paper presents element concentrations and stable sulfur (and in one site lead) isotope ratios in rocks, detailed soil profiles, and plant bioindicators including epiphytic lichen hypogymnia physodes (l.) nyl., mosses (pleurozium schreberi (brid.) mitt., hypnum cupressiforme hedw. s. str., hylocomium splendens (hedw.) b.s.g.), scots pine (pinus sylvestris l.), common ...200516308778
effects of composted sewage sludge on microbial biomass, activity and pine seedlings in nursery forest.the investigation was carried out in a 2 year experiment to evaluate the benefits and hazards of the use of composted sewage sludge as a restoration agent for the soil of the nursery forest intended for growing pinus sylvestris seedlings. the grey forest soil (haplic greyzem) was amended with compost at the 25, 50, 75, 100, 150 and 175 t ha(-1) application rates on a dry matter basis. the organic matter content increased with the increase in sludge amendment as well as the metal content. however ...200616307873
copper-adapted suillus luteus, a symbiotic solution for pines colonizing cu mine spoils.natural populations thriving in heavy-metal-contaminated ecosystems are often subjected to selective pressures for increased resistance to toxic metals. in the present study we describe a population of the ectomycorrhizal fungus suillus luteus that colonized a toxic cu mine spoil in norway. we hypothesized that this population had developed adaptive cu tolerance and was able to protect pine trees against cu toxicity. we also tested for the existence of cotolerance to cu and zn in s. luteus. isol ...200516269769
species-specific allometric scaling under self-thinning: evidence from long-term plots in forest stands.experimental plots covering a 120 years' observation period in unthinned, even-aged pure stands of common beech (fagus sylvatica), norway spruce (picea abies), scots pine (pinus sylvestris), and common oak (quercus petraea) are used to scrutinize reineke's (1933) empirically derived stand density rule [see text], n=tree number per unit area, [see text]=mean stem diameter), yoda's (1963) self-thinning law based on euclidian geometry ([see text] [see text]=mean biomass per tree), and basic assumpt ...200616247620
13c-isotopic fingerprint of pinus pinaster ait. and pinus sylvestris l. wood related to the quality of standing tree mass in forests from nw spain.pine forest plantations of pinus pinaster ait. and p. sylvestris l. located in galicia, nw spain, were selected to study the 13c/12c-isotopic fingerprint in wood core samples in order to find possible relationships between the delta(13)c at natural abundance levels and the quality of the standing tree mass. for each pine species, 24 forests growing on acidic soils were studied: half developed over granite and half over schists. two dominant trees from each plot, corresponding to all possible com ...200516208761
fungal communities in mycorrhizal roots of conifer seedlings in forest nurseries under different cultivation systems, assessed by morphotyping, direct sequencing and mycelial isolation.fungi colonising root tips of pinus sylvestris and picea abies grown under four different seedling cultivation systems were assessed by morphotyping, direct sequencing and isolation methods. roots were morphotyped using two approaches: (1) 10% of the whole root system from 30 seedlings of each species and (2) 20 randomly selected tips per plant from 300 seedlings of each species. the first approach yielded 15 morphotypes, the second yielded 27, including 18 new morphotypes. the overall community ...200516177926
expression of transgenic stilbene synthases in wheat causes the accumulation of unknown stilbene derivatives with antifungal activity.the expression of foreign phytoalexins in a new host is thought to increase fungal resistance, since host-specific pathogens have not experienced selection for detoxifying or metabolising the novel antifungal compounds. two resveratrol synthase genes vst1 and vst2 from grapevine (vitis vinifera l.) and the pinosylvin synthase gene pss from pine (pinus sylvestris l.) were stably transformed into bread wheat. the expression of the target genes is regulated by stress-inducible grapevine promoters. ...200516173460
response surface optimization of the removal of nickel from aqueous solution by cone biomass of pinus sylvestris.response surface methodology was applied to optimize the removal efficiency of ni(ii). pinus sylvestris ovulate cones were used in this study. a 2(3) full-factorial central composite design was employed for experimental design and analysis of the results. the initial ni(ii) concentration (10-30 mg/l), ph (2.5-6.5) and biomass concentration (5-25 g/l) were the critical components of the removal optimized. the optimum ph, m (biomass concentration) and c0 (initial ni(ii) concentration) were found t ...200616162409
[effects of polyethylene glycol (peg)-simulated drought stress on pinus sylvestris var. mongolica seed germination on sandy land].with introduced sandy land mongolian pine (pinus sylvestris var. mongolica) seeds as test material, this paper studied the effects of polyethylene glycol (peg)-simulated drought stress on their germination. the results showed that peg could retard the germination of stressed seeds, and the germination rate, germination index, and germination potential were all decreased with increasing peg concentration. the seeds treated with 30% peg (about -1.20 mpa water potential) could not germinate at all ...200516110648
[variability of the cytological parameters of pinus sylvestris l. seeds from the unique hrenovskoy pine forest].hrenovskoy pine forest is a unique island stand at the boundary of the species range of scots pine pinus sylvestris l. this object is of exceptional economic value, because it serves as a forest-seed base for the voronezh oblast and some other regions of russia; therefore, the stand and seed qualities have to be monitored constantly. the results of the first cytogenetic study of the seed progeny of p. sylvestris from the morozov grove, a high-quality stand in a reserved site within the hrenovsko ...200516080602
variation in needle longevity is related to needle-fascicle production rate in pinus sylvestris.latitudinal variation in needle longevity of conifers in response to climatic variability is a well-known phenomenon, but its significance has only rarely been studied. scots pine (pinus sylvestris l.) stands were investigated in four locations in lapland (northern finland and northern sweden, 67-68 degrees n) and in four locations in estonia (59 degrees n) to test the hypothesis that plasticity in needle longevity results in similar needle biomass per foliated shoot length across latitudes. the ...200516076775
seasonal patterns of ascorbate in the needles of scots pine (pinus sylvestris l.) trees: correlation analyses with atmospheric o3 and no2 gas mixing ratios and meteorological parameters.in the present field study the role of ascorbate in scavenging the harmful atmospheric trace gases o3 and no2 was examined. for this purpose ascorbate contents were determined in needles of adult scots pine trees (pinus sylvestris l.) during three consecutive years. ascorbate contents were correlated with ambient tropospheric o3 and no2 concentrations and with meteorological parameters. the results showed a strong correlation of atmospheric o3 but not of atmospheric no2 concentrations with the a ...200616051404
comparison of the moss pleurozium schreberi with needles and bark of pinus sylvestris as biomonitors of pollution by industry in stalowa wola (southeast poland).concentrations of heavy metals were determined in the terrestrial bryophyte pleurozium schreberi and in samples of bark and current and previous year needles of pinus sylvestris collected along transects around the stalowa wola industry center (southeast poland) and compared with material of the same species from a control site. the suitability of bark and pine needles for use in monitoring of serious heavy metal pollution was investigated. in the examined area current and previous year pine nee ...200616029892
long-term evaluation of the needle surface wax condition of pinus sylvestris around different industries in lithuania.the aim of our study was to evaluate the annual dynamics of needle surface wax erosion and wettability in scots pines exposed to a gradient of industrial pollutants emitted from the main factories of lithuania: a nitrogen fertilizer factory, an oil refinery and a cement factory. decreased emissions (in the case of the oil refinery and the cement factory) were reflected in the increased structural surface area (ssa, i.e. area covered by tubular waxes) on the needles. the nearly constant amount of ...200516005772
changes in the concentrations of phenolics and photosynthates in scots pine (pinus sylvestris l.) seedlings exposed to nickel and copper.studies were done on the effects of elevated soil concentrations of copper (cu) and (ni) on foliar carbohydrates and phenolics in scots pine (pinus sylvestris l.). four year-old seedlings were planted in pots filled with metal-treated mineral forest soil in early june. the experimental design included all combinations of four levels of cu (0, 25, 40 and 50 mg kg(-1) soil dw) and ni (0, 5, 15 and 25 mg kg(-1) soil dw). current year needles were sampled for soluble sugar, starch and phenolics at t ...200516005771
meteorological factors and air pollution in lithuanian forests: possible effects on tree condition.this study investigates changes in tree condition and environmental factors in lithuania during the active growing season in 1991-2001. the average crown defoliation and the proportion of healthy trees of pinus sylvestris, picea abies, betula sp., fraxinus excelsior, alnus incana, alnus glutinosa, populus tremula, and quercus robur, meteorological (average temperature, amount of precipitation, hydrothermal coefficient) and air pollution data (acidity of precipitation, concentrations of so2, no2 ...200516005769
regeneration patterns in boreal scots pine glades linked to cold-induced photoinhibition.regeneration patterns of pinus sylvestris l. juveniles in central siberian glades were studied in relation to cold-induced photoinhibition. spatial distribution of seedlings in different height classes revealed higher seedling densities beneath the canopy than beyond the canopy, and significantly higher densities of seedlings < 50 cm tall on the north side of the trees. these patterns coincided with differences in light conditions. compared with plants on the north side of canopy trees (north-ex ...200515996957
indications of limited altered gene expression in pinus sylvestris trees from the chernobyl region.to evaluate the consequences of irradiation on the vegetation of the chernobyl region, gene expression was compared in morphologically normal and dwarf needles from the same pinus sylvestris trees in a region where the absorbed dose was 3-5 gy. to compare the levels of gene expression, arrays consisting of 373 pinus taeda cdnas were hybridized with labeled cdna derived from normal and dwarf needles of p. sylvestris. twelve genes were significantly (p<0.01) up- or down-regulated between normal an ...200515961195
measuring the genetic structure of the pollen pool as the probability of paternal identity.contemporary pollen flow in forest plant species is measured by the probability of paternal identity (ppi) for two randomly sampled offspring, drawn from a single female, and contrasting that with ppi for two random offspring, drawn from different females. two different estimation strategies have emerged: (a) an indirect approach, using the 'genetic structure' of the pollen received by different mothers and (b) a direct approach, based on parentage analysis. the indirect strategy is somewhat lim ...200515940275
comparing bayesian estimates of genetic differentiation of molecular markers and quantitative traits: an application to pinus sylvestris.comparison of the level of differentiation at neutral molecular markers (estimated as f(st) or g(st)) with the level of differentiation at quantitative traits (estimated as q(st)) has become a standard tool for inferring that there is differential selection between populations. we estimated q(st) of timing of bud set from a latitudinal cline of pinus sylvestris with a bayesian hierarchical variance component method utilizing the information on the pre-estimated population structure from neutral ...200515940273
effects of timing of soil frost thawing on scots pine.effects of the timing of soil thawing in the spring on scots pine (pinus sylvestris l.) were studied under controlled laboratory conditions. sixteen 6-year-old saplings were lifted from the field, replanted in containers and placed in four treatments in controlled environment (ce) chambers with four replicate saplings per chamber. the saplings were held in the ce chambers during one simulated winter and one simulated growing season. the soil was frozen to -2 degrees c during a second simulated w ...200515929936
removal of lead (ii) ions from synthetic and real effluents using immobilized pinus sylvestris sawdust: adsorption on a fixed-bed column.the purpose of this work was to evaluate the potential of pinus sylvestris sawdust, in a continuous flow removal of lead (ii) ions from synthetic and industrial aqueous effluents. the kinetic parameters obtained in a batch process were used to scale-up the process on a mini-column and to choose the breakthrough model. the column experimental data concerning the volumes treated were correlated using the bed depth service time model. these experimental data closely fitted the bed depth service tim ...200515894423
antioxidative activity of volatile extracts isolated from angelica tenuissimae roots, peppermint leaves, pine needles, and sweet flag leaves.volatile extracts were isolated from dried medicinal plants [angelica tenuissimae roots (at, angelica tenuissima nakai), peppermint leaves (pl, mentha arvensis l.), pine needles (pn, pinus sylvestris l.), and sweet flag leaves (sf, acorus gramineus rhizoma)] using steam distillation under reduced pressure, followed by continuous liquid-liquid extraction (drp-lle). the extracts were then analyzed by gas chromatography (gc) and gas chromatography-mass spectrometry (gc-ms). the major volatile const ...200515884849
insect egg deposition induces defence responses in pinus sylvestris: characterisation of the elicitor.egg deposition by the phytophagous sawfly diprion pini l. (hymenoptera, diprionidae) is known to induce locally and systemically the emission of volatiles in scots pine (pinus sylvestris l.) that attract the egg parasitoid chrysonotomyia ruforum krausse (hymenoptera, eulophidae). the egg parasitoids kill the eggs and thus prevent damage to the plant from feeding sawfly larvae. the elicitor inducing the pine's response is known to be located in the oviduct secretion which the female sawfly applie ...200515879065
the upward shift in altitude of pine mistletoe (viscum album ssp. austriacum) in switzerland--the result of climate warming?pine mistletoe (viscum album ssp. austriacum) is common in natural scots pine (pinus sylvestris l.) forests in the alpine rhone valley, switzerland. this semi-parasite, which is regarded as an indicator species for temperature, increases the drought stress on trees and may contribute to the observed pine decline in the region. we recorded mistletoes on representative plots of the swiss national forest inventory ranging from 450 to 1,550 m a.s.l. we found mistletoe on 37% of the trees and on 56% ...200515875222
estimating foliage biomass in scots pine (pinus sylvestris) and norway spruce (picea abies) plots.dynamic decomposition models are needed to estimate changes in the carbon stock of boreal soil because these changes are difficult to measure directly. an important aboveground carbon flux to the soil is foliage litterfall. to estimate this flux, both the amount and the turnover rate of the foliage biomass component must be known. several methods for estimating foliage biomass of scots pine (pinus sylvestris l.) and norway spruce (picea abies (l.) karst.), including biomass equations and biomass ...200515870050
analysis of biomass accumulation and stem size distributions over long periods in managed stands of pinus sylvestris in finland using the 3-pg model.we tested the performance of a process-based model (pbm) in relation to long-term mensuration data from two sites in finland where the stands were up to 90 years old and had been thinned at approximately 5-year intervals over the last 50 years. the pbm used was based on the 3-pg (physiological principles to predict growth) model developed by landsberg and waring (1997), with modifications in the biomass allocation routine, for which we used data and calculations by vanninen (2003) to estimate th ...200515870048
elemental analyses of pine bark and wood in an environmental study.bark and wood samples were taken from the same individuals of scots pine (pinus sylvestris l.) from a polluted area close to a cu-ni smelter in harjavalta and from some relatively unpolluted areas in western finland. the samples were analysed by thick-target particle induced x-ray emission (pixe) after preconcentration by dry ashing at 550 degrees c. the elemental contents of pine bark and wood were compared to study the impact of heavy metal pollution on pine trees. by comparison of the element ...200415862848
[decline regularity and causes of pinus sylvestris var. mongolica plantation on sandy land].the study showed that the decline of pinus sylvestris var. mongolica plantation out of its natural distribution area was a phenomenon of premature senility. the infected harm of shoot blight was the direct cause of pinus sylvestris var. mongolica dying, but wasn't the basic reason. the decline of pinus sylvestris var. mongolica was caused by the interaction of biological and other factors, which was "the third disease" beyond infectious and non-infectious diseases, namely, "decline disease of fo ...200415825431
[major features of decline of pinus sylvestris var. mongolica plantation on sandy land].in view of the decline of man-made sand-fixation forest of pinus sylvestris var. mongolica in zhanggutai sand land of liaoning province, this paper studied the major characteristics of the decline. the appearance of the declining man-made sand-fixation forest of pinus sylvestris var. mongolica was grey green, its needle leaf was very thin, the blooming and fruiting rate was low, the average quantity of cones per tree was only 10.4-16.5, with only 6.96 g to 7.39 g per thousand seeds, and there we ...200415825430
a plant notices insect egg deposition and changes its rate of photosynthesis.scots pine (pinus sylvestris) is known to change its terpenoid metabolism in response to egg deposition by the sawfly diprion pini (hymenoptera, diprionidae). three days after egg deposition, parts of the pine twig adjacent to the egg-laden one are induced to emit volatiles, which attract egg parasitoids. in this study, we investigated whether egg deposition by this sawfly affects pine photosynthesis. measurements of photosynthesis were taken from untreated control twigs and from pine twigs adja ...200515821143
free radical generation in pinus sylvestris and larix decidua seeds primed with polyethylene glycol or potassium salt solutions.electron paramagnetic resonance (epr) spectra of pinus sylvestris and larix decidua seeds show that priming with peg+200 mg kg(-1) gibberelic acid (ga(3)) results in appreciably higher free radical contents than in unprimed control samples. only relatively minor changes in the free radical levels were observed in seeds primed with k(+) salts. however, both priming treatments have been reported previously to result in faster germination rates compared to controls without changing the germination ...200515820658
[population-genetic variation in scots pine pinus sylvestris l. from the main forest regions of ukraine].using electrophoretic analysis of 22 isozyme loci controlling ten enzyme systems, we studied intrapopulation and interpopulation variation of scots pine pinus sylvestris l. in the main forest regions of ukraine. in 15 of the populations examined, 76.5% of genes were polymorphic, and an average plant was shown to be heterozygous at 23.4% of the genes. the lowest and highest values of major polymorphism parameters were characteristic of respectively the relic populations of ukrainian carpathians a ...200515810611
in-vitro anti-inflammatory activity of pinus sylvestris and plantago lanceolata extracts: effect on inducible nos, cox-1, cox-2 and their products in j774a.1 murine macrophages.extracts of the plant species pinus sylvestris l. and plantago lanceolata l. have been used in traditional medicine for the treatment of certain respiratory diseases, but little is known about their precise effects and mechanisms of action. in this study, we investigated the effect of these plant extracts on the production of nitric oxide (no) and prostaglandin e(2), no synthase (nos) type ii, cyclooxygenase-1 (cox-1) and cox-2 mrna expression in the murine macrophage cell line j774a.1. we found ...200515807995
allometry and evaluation of in situ optical lai determination in scots pine: a case study in belgium.we evaluated several optical methods for in situ estimation of leaf area index (lai) in a belgian scots pine (pinus sylvestris l.) stand. the results obtained were compared with lai determined from allometric relationships established in the same stand. we found high correlations between branch cross-sectional area, diameter at breast height (dbh) and basal area as dependent variables, and leaf mass, needle area and crown projection as independent variables. we then compared lai estimated by all ...200515805092
contrasting net primary productivity and carbon distribution between neighboring stands of quercus robur and pinus sylvestris.standing biomass, net primary production (npp) and soil carbon (c) pools were studied in a 67-year-old pedunculate oak (quercus robur l.) stand and a neighboring 74-year- old scots pine (pinus sylvestris l.) stand in the belgian campine region. despite a 14% lower tree density and a lower tree height in the oak stand, standing biomass was slightly higher than in the pine stand (177 and 169 mg ha(-1) in oaks and pines, respectively), indicating that individual oak trees contained more biomass tha ...200515805090
uranium distribution and cycling in scots pine (pinus sylvestris l.) growing on a revegetated u-mining heap.we determined the uranium distribution in soil and its allocation in compartments of 35-year-old scots pine developed on a revegetated u-mining heap. the processes controlling the dynamics of u recycling were identified and further quantified in terms of annual fluxes. as pine developed, an acid humus layer emerged leading to weathering of the alkaline mining debris but this had little effect on u mobility in the soil profile. increased u mobility mainly involved a translocation of u to metal-hu ...200515795035
enhancement of attraction and trap catches of the old-house borer, hylotrupes bajulus (coleoptera: cerambycidae), by combination of male sex pheromone and monoterpenes.monoterpenoid compounds extracted from wood of scots pine, pinus sylvestris l, synergized the attraction of the old-house borer, hylotrupes bajulus (l), to the male pheromone (3r)-3-hydroxy-2-hexanone ((3r)-ketol) + 1-butanol. glasshouse experiments using ground traps baited with extracts derived from scots pine wood or the monoterpenes (+)-alpha-pinene, (-)-verbenone, (-)-trans-pinocarveol and (+)-terpinen-4-ol attracted significantly more h bajulus females, but caught fewer of them, than the s ...200515776406
chitosan (biochikol 020 pc) in the control of some ornamental foliage diseases.chitosan, (biochikol 020 pc) a potential elicitor of plant defence and also an active inhibitor of fungal growth was used in experiments. the compound was used at concentrations 0.01 to 0.2% as a plant spray for rose protection against sphaerotheca pannosa var. rosae, peronospora sparsa and diplocarpon rosae, oidium chrysanthemi and puccinia horiana on chrysanthemum, against melampsora epitea on willow. myrothecium roridum on dieffenbachia and against lophodermium spp on scots pine. effectivenes ...200415756862
flavonol 3-o-glycoside hydroxycinnamoyltransferases from scots pine (pinus sylvestris l.).flavonol 3-o-glucosides esterified with ferulic or p-coumaric acid at positions 3'' and 6'' are the major uv-b screening pigments of the epidermal layer of scots pine (pinus sylvestris) needles. the last steps in the biosynthesis of these compounds are catalyzed by enzymes that transfer the acyl part of hydroxycinnamic acid coa esters to flavonol 3-o-glucosides. a newly developed enzyme assay revealed three flavonol 3-o-glucoside hydroxycinnamoyltransferases (hcts) in scots pine needles with spe ...200515752358
reciprocal controlled crosses between pinus sylvestris and p. mugo verified by a species-specific cpdna marker.a species-specific marker of cpdna (paternally inherited in pines) was used to verify the hybrid origin of seedlings from controlled reciprocal crosses between pinus sylvestris and p. mugo. a very low degree of compatibility between those two species has been revealed. in the three consecutive years of experiments, no filled seeds were obtained in the combination with p. mugo as the seed parent. from p. sylvestris as the seed parent and p. mugo as the pollen donor, we succeeded to obtain four fi ...200515741663
soil respiration in a mixed temperate forest and its contribution to total ecosystem respiration.soil respiration (sr) was measured with an infrared gas analyzer in nine plots representative of the heterogeneous vegetation in a mixed coniferous-deciduous forest in the belgian campine region. selected plots included the two most representative overstory species (pinus sylvestris l. and quercus robur l.) in combination with the most representative understory species of the forest. a model that includes temperature and water as the main controlling variables was fitted to the data. we found la ...200515741145
trace elements in fruiting bodies of ectomycorrhizal fungi growing in scots pine (pinus sylvestris l.) stands in poland.the trace metal contents in fruiting bodies of ectomycorrhizal (ecm) fungi, symbiotic partners of scots pine, were studied on three sites situated in west-central poland. elements were determined by atomic absorption spectrometry in 123 samples of 16 species. the study explored the differences in metal accumulation in relation to site, fungal species, age and part of the fruiting body and results were related to metal content in soil and plant material (roots and needles). soil analysis revealed ...200515740762
pine microsatellite markers allow roots and ectomycorrhizas to be linked to individual trees.linking roots and ectomycorrhizas (ecm) to individual host trees in the field is required to test whether individual trees support different ectomycorrhizal communities. here we describe a method that identifies the source of ecm roots by pcr of polymorphic pine nuclear microsatellite loci using fluorescently labelled primers and high-throughput fragment analysis. its-pcr can also be performed on the same ecm dna extract for fungal identification. the method was tested on five neighbouring scots ...200515720641
[electrical impedance spectroscopy method for measuring cold hardiness of plants].electrical impedance spectroscopy (eis) is a method for measuring cold hardiness of plants. it has been widely used in the fields of agriculture, forestry and horticulture. in this paper the following aspects were introduced and discussed: (1) physical and physiological factors of impedance measurements in plants, (2) suitable models for measuring eis, and (3) method for assessing cold hardiness by means of eis. in traditional eis analysis, after completion of the artificially controlled freezin ...200515692174
phenolic extractives from the bark of pinus sylvestris l. and their effects on inflammatory mediators nitric oxide and prostaglandin e2.the anti-inflammatory properties of phenolic pine (pinus sylvestris l.) bark extract were studied. the pine bark extract was fractionated by liquid-liquid extractions and semipreparative high-performance liquid chromatography to reveal the most potent constituents. the phenolic compositions of three pine bark samples obtained, a crude extract, a chloroform fraction, and a semipreparative fraction, were analyzed using high-performance liquid chromatography with uv diode array detection and/or ele ...200415675800
a new dihydroflavonol from pinus sylvestris l.a novel dihydroflavonol, c-6,o-7-dimethylaromadendrin, was isolated from a 70% aqueous acetone extract of pine (pinus sylvestris l.) bark. its structure was determined by high-resolution negative fast atom bombardment mass spectrometry and nmr spectroscopy. the complete assignment of proton and carbon signals was achieved by 2d nmr experiments: hsqc, hmbc, dqf-cosy and noesy.200515674796
carbon budget of pinus sylvestris saplings after four years of exposure to elevated atmospheric carbon dioxide concentration.to study the responses of scots pine (pinus sylvestris l.), a commercially important tree species in europe, to future increases in atmospheric co2 concentration ([co2]), we grew saplings for 4 years in the ground in open-top chambers in ambient or ambient + 400 micromol mol(-1) co2, without supplemental addition of nutrients and water. carbon (c) budgets were developed for trees in both co2 treatments based on productivity and biomass data obtained from destructive harvests at the end of the th ...200515631981
seasonal variations in location and population structure of endophytes in buds of scots pine.we studied the location and distribution of a bacterial isolate, a mycobacterium sp., in buds of scots pine (pinus sylvestris l.). using a probe specific for the 16s rrna of the mycobacterium sp., the bacterium was found by in situ hybridization in the meristematic tissues of 40% of all bud samples examined. because we had previously found other bacterial and fungal endophytes in the meristematic tissues of scots pine buds, we studied their occurrence in buds during shoot development and dormanc ...200515631977
plant resistance to cold stress: mechanisms and environmental signals triggering frost hardening and dehardening.this introductory overview shows that cold, in particular frost, stresses a plant in manifold ways and that the plant's response, being injurious or adaptive, must be considered a syndrome rather than a single reaction. in the course of the year perennial plants of the temperate climate zones undergo frost hardening in autumn and dehardening in spring. using scots pine (pinus sylvestris l.) as a model plant the environmental signals inducing frost hardening and dehardening, respectively, were in ...200415625401
gas chromatography with mass spectrometric and electroantennographic detection: analysis of wood odorants by direct coupling of insect olfaction and mass spectrometry.a gas chromatography-mass spectrometry-electroantennographic detection (gc-ms/ead) setup has been designed by adapting a commercially available "olfactory detector port" to the use with an insect antenna. measurements were performed with antennae of the old house borer hylotrupes bajulus, a widespread insect pest of coniferous timbers. headspace volatiles from timber of pinus sylvestris were collected and analysed by gc-ms. about 30 compounds were identified in the kovacs range from 500 to 1200, ...200415595552
microfibril angle in wood of scots pine trees (pinus sylvestris) after irradiation from the chernobyl nuclear reactor accident.the secondary cell wall structure of tracheids of scots pine (pinus sylvestris l.), especially the angle of microfibrils in the s(2) layer, was examined in wood deposited prior to and after the chernobyl accident in 1986. microscopic analysis was carried out on wood samples collected in october 1997 from breast height of three pine trees 16, 30 and 42 years old. the polluted site was located in a distance of 5 km south from the chernobyl nuclear power plant where radioactive contamination in 199 ...200515589646
modeling tree water deficit from microclimate: an approach to quantifying drought stress.tree water deficit estimated by measuring water-related changes in stem radius (deltaw) was compared with tree water deficit estimated from the output of a simple, physiologically reasonable model (deltawe), with soil water potential (psisoil) and atmospheric vapor pressure deficit (vpd) as inputs. values of deltaw were determined by monitoring stem radius changes with dendrometers and detrending the results for growth. we followed changes in deltaw and deltawe in pinus sylvestris l. and quercus ...200515574396
effect of attract and kill formulations and application rates on trap catches of european pine shoot moth (lepidoptera: tortricidae) and shoot damage in scots pine saplings.attract and kill technology was tested for management of european pine shoot moth, rhyacionia buoliana (denis & schiffermüller), in 4-6-yr-old scots pine, pinus sylvestris l., plantations managed by jablonna and pultusk forest districts, poland. in 2001, two formulations based on ricinoleic acid and hydrocarbon fraction (petroleum jelly) in combination with (e)-9-dodecenyl acetate, the sex pheromone of the pine shoot moth; permethrin as a contact insecticide; and tinuvin uv absorber were used. i ...200415568351
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