Publications

plant water stress effects on stylet probing behaviors of homalodisca vitripennis (hemiptera: cicadellidae) associated with acquisition and inoculation of the bacterium xylella fastidiosa.	abstract the glassy-winged sharpshooter, homalodisca vitripennis (germar) (hemiptera: cicadellidae), is a xylem fluid-ingesting leafhopper that transmits xylella fastidiosa wells et al., a plant-infecting bacterium that causes several plant diseases in the americas. although the role of plant water stress on the population density and dispersal ofh. vitripennis has been studied, nothing is known about the effects of plant water stress on the transmission of x. fastidiosa by h. vitripennis. a lab ...	2014	24665686
preliminary examination of herbal extracts on the inhibition of helicobacter pylori.	this work aims at describing the traditional uses, to determine the antimicrobial potential of two different extracts hexane, acetone of the leaves of citrus unshiu, citrus sinensis, citrus limon, laurus nobilis, citrus paradisi on clinical strain of h. pylori in a bid to identify potential sources of cheap starting materials for the synthesis of new drugs. h. pylori strain was a culture collection of hacettepe university, turkey.	2014	24653559
antioxidant and antifungal activity of extracts of the aerial parts of thymus capitatus (l.) hoffmanns against four phytopathogenic fungi of citrus sinensis.	many medicinal plants from the lamiaceae family can be easily found in algeria. these plants have been used as traditional medicines by local ethnic groups. thymus capitatus is known in algeria as "zaatar" and has been commonly used as a spice, and reported to have many biological effects.	2014	24644439
physicochemical and functional properties of micronized jincheng orange by-products (citrus sinensis osbeck) dietary fiber and its application as a fat replacer in yogurt.	orange by-products from juice extraction are generally discarded or used in animal feed due to their low market value. however, orange by-products show potential as dietary fiber (df) and fat replacers in products such as yogurt. this study assessed the benefits of using orange by-products in df-enriched materials such as df powders (op) and micronized df with ball-milling (mdf). the study also investigated the effects of adding different levels of op and mdf on the quality of low-fat yogurt. re ...	2014	24625022
operating conditions for the continuous bioremediation of free cyanide contaminated wastewater using aspergillus awamori.	generation of cyanide-containing wastewater is a growing problem worldwide as numerous cyanide complexes are highly unstable and degrade to form free cyanide (f-cn), the most toxic form of cyanide. agro-waste materials, such as sweet orange (citrus sinensis) waste from the citrus industry, are rich in readily metabolisable carbohydrates that can supplement microbial activity and thus support biodegradation of toxic compounds in wastewater. this study reports on optimal operating conditions for t ...	2014	24622547
temporal progression of 'candidatus liberibacter asiaticus' infection in citrus and acquisition efficiency by diaphorina citri.	over the last decade, the plant disease huanglongbing (hlb) has emerged as a primary threat to citrus production worldwide. hlb is associated with infection by phloem-limited bacteria ('candidatus liberibacter' spp.) that are transmitted by the asian citrus psyllid, diaphorina citri. transmission efficiency varies with vector-related aspects (e.g., developmental stage and feeding periods) but there is no information on the effects of host-pathogen interactions. here, acquisition efficiency of 'c ...	2014	24620723
an integrative analysis of the transcriptome and proteome of the pulp of a spontaneous late-ripening sweet orange mutant and its wild type improves our understanding of fruit ripening in citrus.	fruit ripening is a complex, genetically programmed process that occurs in conjunction with the differentiation of chloroplasts into chromoplasts and involves changes to the organoleptic properties of the fruit. in this study, an integrative analysis of the transcriptome and proteome was performed to identify important regulators and pathways involved in fruit ripening in a spontaneous late-ripening mutant ('fengwan' orange, citrus sinensis l. osbeck) and its wild type ('fengjie 72-1'). at the t ...	2014	24600016
the post-transcriptional regulator rsma/csra activates t3ss by stabilizing the 5' utr of hrpg, the master regulator of hrp/hrc genes, in xanthomonas.	the rsma/csra family of the post-transcriptional regulators of bacteria is involved in the regulation of many cellular processes, including pathogenesis. in this study, we demonstrated that rsma not only is required for the full virulence of the phytopathogenic bacterium xanthomonas citri subsp. citri (xcc) but also contributes to triggering the hypersensitive response (hr) in non-host plants. deletion of rsma resulted in significantly reduced virulence in the host plant sweet orange and a delay ...	2014	24586158
differential transcriptional regulation of l-ascorbic acid content in peel and pulp of citrus fruits during development and maturation.	citrus fruits are an important source of ascorbic acid (asa) for human nutrition, but the main pathways involved in its biosynthesis and their regulation are still not fully characterized. to study the transcriptional regulation of asa accumulation, expression levels of 13 genes involved in asa biosynthesis, 5 in recycling and 5 in degradation were analyzed in peel and pulp of fruit of two varieties with different asa concentration: navel orange (citrus sinensis) and satsuma mandarin (citrus uns ...	2014	24567029
isolation, classification and transcription profiles of the ap2/erf transcription factor superfamily in citrus.	the ap2/erf gene family encodes plant-specific transcription factors. in model plants, ap2/erf genes have been shown to be expressed in response to developmental and environmental stimuli, and many function downstream of the ethylene, biotic, and abiotic stress signaling pathways. in citrus, ethylene is effective in regulation citrus fruit quality, such as degreening and aroma. however, information about the citrus ap2/erf family is limited, and would enhance our understanding of fruit responses ...	2014	24566692
identification of putative tal effector targets of the citrus canker pathogens shows functional convergence underlying disease development and defense response.	transcriptional activator-like (tal) effectors, formerly known as the avrbs3/ptha protein family, are dna-binding effectors broadly found in xanthomonas spp. that transactivate host genes upon injection via the bacterial type three-secretion system. biologically relevant targets of tal effectors, i.e. host genes whose induction is vital to establish a compatible interaction, have been reported for xanthomonads that colonize rice and pepper; however, citrus genes modulated by the tal effectors pt ...	2014	24564253
characterization of mango (mangifera indica l.) transcriptome and chloroplast genome.	we characterized mango leaf transcriptome and chloroplast genome using next generation dna sequencing. the rna-seq output of mango transcriptome generated >12 million reads (total nucleotides sequenced >1 gb). de novo transcriptome assembly generated 30,509 unigenes with lengths in the range of 300 to ≥3,000 nt and 67× depth of coverage. blast searching against nonredundant nucleotide databases and several viridiplantae genomic datasets annotated 24,593 mango unigenes (80% of total) and identifi ...	2014	24515595
furocoumarins and coumarins photoinactivate colletotrichum acutatum and aspergillus nidulans fungi under solar radiation.	the increasing tolerance to currently-used fungicides is a major problem both in clinical and agricultural areas leading to an urgent need for the development of novel antifungal strategies. this study investigated the in vitro antimicrobial photo treatment (apt) of conidia of the plant-pathogenic fungus colletotrichum acutatum and the ascomycete aspergillus nidulans with the furocoumarins 8-methoxypsoralen (8-mop) and isopimpinellin, and a mixture of two coumarins (7-methoxy coumarin and citrop ...	2014	24509069
oral dose of citrus peel extracts promotes wound repair in diabetic rats.	diabetic patients wound healing is slower than the healthy individuals. three citrus peel extracts; lemon (citrus limon), grapes fruits (citrus paradise) and orange (citrus sinensis) promote wound healing in experimental animals. this study investigated the effect of oral treatment with citrus peel extracts on wound repair of the skin of diabetic rats. the extracts were estimated for vitamin c and total carotenoid contents prior to animal study. diabetes mellitus was induced in rats by intraperi ...	2013	24506007
postharvest control of western flower thrips (thysanoptera: thripidae) and california red scale (hemiptera: diaspididae) with ethyl formate and its impact on citrus fruit quality.	the postharvest control of arthropod pests is a challenge that the california citrus industry must overcome when exporting fruit overseas. currently, methyl bromide fumigation is used to control postharvest pests on exported citrus, but it may soon be unavailable because of use restrictions and cost of this health-hazard ozone-depleting chemical. ethyl formate is a natural plant volatile and possible alternative to methyl bromide in postharvest insect control. the objectives of this study were 1 ...	2013	24498732
scanning electron-microscopy of abscission-zone surfaces of valencia-orange fruit.	the scanning electron-microscope was used to obtain a more adequate view of the separation surfaces exposed when the stem was pulled from the fruit of valencia orange. separations were made at 0, 24 and 48 h after ethylene treatment of the fruit. the most noticeable differences are the lessened amount of tearing in proximal vascular bundles at 48 h and decreased rupture of distal parenchyma cells first observed at 24 h.	1971	24493280
comparative analysis of surface wax in mature fruits between satsuma mandarin (citrus unshiu) and 'newhall' navel orange (citrus sinensis) from the perspective of crystal morphology, chemical composition and key gene expression.	surface wax of mature satsuma mandarin (citrus unshiu) and 'newhall' navel orange (citrus sinensis) was analysed by crystal morphology, chemical composition, and gene expression levels. the epicuticular and total waxes of both citrus cultivars were mostly composed of aldehydes, alkanes, fatty acids and primary alcohols. the epicuticular wax accounted for 80% of the total wax in the newhall fruits and was higher than that in the satsuma fruits. scanning electron microscopy showed that larger and ...	2014	24491718
characterization of the glutathione s-transferase gene family through ests and expression analyses within common and pigmented cultivars of citrus sinensis (l.) osbeck.	glutathione s-transferases (gsts) represent a ubiquitous gene family encoding detoxification enzymes able to recognize reactive electrophilic xenobiotic molecules as well as compounds of endogenous origin. anthocyanin pigments require gsts for their transport into the vacuole since their cytoplasmic retention is toxic to the cell. anthocyanin accumulation in citrus sinensis (l.) osbeck fruit flesh determines different phenotypes affecting the typical pigmentation of sicilian blood oranges. in th ...	2014	24490620
citrus sinensis annotation project (cap): a comprehensive database for sweet orange genome.	citrus is one of the most important and widely grown fruit crop with global production ranking firstly among all the fruit crops in the world. sweet orange accounts for more than half of the citrus production both in fresh fruit and processed juice. we have sequenced the draft genome of a double-haploid sweet orange (c. sinensis cv. valencia), and constructed the citrus sinensis annotation project (cap) to store and visualize the sequenced genomic and transcriptome data. cap provides gbrowse-bas ...	2014	24489955
determination of allergenic hydroperoxides in essential oils using gas chromatography with electron ionization mass spectrometry.	fragrance monoterpenes are widely used commercially due to their pleasant scent. in previous studies, we have shown that air-exposed monoterpenes form hydroperoxides that are strong skin sensitizers. methods for detection and quantification of the hydroperoxides in essential oils and scented products are thus desirable. due to thermolability and low uv absorbance, this is a complicated task. we have recently developed a sensitive lc-esi-ms method, but with limited structural information and sepa ...	2014	24482420
adventive plants from ovules and nucelli in citrus.	1- to 8-week-old ovules and nucelli from three citrus cultivars-shamouti and valencia (citrus sinensis) oranges and marsh seedless (c. paradisi) grapefruit-were cultured in vitro. no embryo differentiation was observed in the explants prior to culture. the shamouti ovules had degenerated and were apparently unfertilized. embryoids formed on murashige and tucker nutrient medium supplemented with 500 mg/l malt extract. whole plants developed on the same basal medium supplemented with kinetin and i ...	1972	24477995
comparative de novo transcriptome analysis and metabolic pathway studies of citrus paradisi flavedo from naive stage to ripened stage.	grapefruit (citrus pardisi) is a popular citrus fruit that is a cross between a sweet orange and pummelo. this research article focuses on an in silico approach for comparative analysis of c. paradisi green flavedo (gf) and ethylene treated flavedo (etf) transcriptome data. our pathway analysis provides comprehensive information of genes playing significant role in different stages of ripening in fruit. de novo assembly was carried out using six different assemblers namely gs assembler, seqman n ...	2014	24477585
antioxidant potential of a polyherbal antimalarial as an indicator of its therapeutic value.	nefang is a polyherbal product composed of mangifera indica (bark and leaf), psidium guajava, carica papaya, cymbopogon citratus, citrus sinensis, and ocimum gratissimum (leaves), used for the treatment of malaria. compounds with antioxidant activity are believed to modulate plasmodial infection. antioxidant activity of the constituent aqueous plants extracts, in vitro, was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (dpph), total phenolic content (tpc), and ferric reducing antioxidant pow ...	2013	24454347
population of endogenous pararetrovirus genomes in carrizo citrange.	the complete genome sequences of three related endogenous pararetroviruses (eprvs) were obtained by 454 sequencing of nucleic acid extracts from carrizo citrange, used as a citrus rootstock. numerous homologous sequences have been found in the sweet orange genome. the new eprvs are most closely related to petunia vein-clearing virus.	2014	24435856
pectin-polygalacturonase in citrus.	extracts from citrus [citrus sinensis (l.) osbeck] pedicles rated as containing polygalacturonase and cellulase gave activities both as measured with carboxymethylcellulose and with sodium polypectate. gel electrophrosis of the extract demonstrated two region areas that contained cellulytic activity.	1976	24430758
evidence of recombinant citrus tristeza virus isolate occurring in acid lime cv. pant lemon orchard in uttarakhand terai region of northern himalaya in india.	the present study for the first time describes biological and molecular characterization of citrus tristeza virus (ctv) occurring in the terai area of uttarakhand state in northern himalaya region of india. direct antigen coated-elisa and reverse transcriptase-polymerase chain reaction (rt-pcr) detected the ctv infection in acid lime cv. pant lemon (citrus aurantifolia) orchards of pantnagar with an estimated disease incidence of 16.6-20.5 %. to know the biological and genetic properties, an iso ...	2013	24426255
recent introduction and recombination in colletotrichum acutatum populations associated with citrus postbloom fruit drop epidemics in são paulo, brazil.	citrus crops in são paulo state, brazil, have been severely affected by postbloom fruit drop disease (pfd), which is caused by colletotrichum acutatum. this disease leads to the drop of up to 100% of young fruits. previous studies have assumed that this pathogen exhibits a clonal reproductive mode, although no population genetic studies have been conducted so far. thus, the genetic structure of six c. acutatum populations from sweet orange orchards showing pfd symptoms was determined using nine ...	2014	24423403
the effects of nigella sativa (ns), anthemis hyalina (ah) and citrus sinensis (cs) extracts on the replication of coronavirus and the expression of trp genes family.	extracts of anthemis hyalina (ah), nigella sativa (ns) and peels of citrus sinensis (cs) have been used as folk medicine to fight antimicrobial diseases. to evaluate the effect of extracts of ah, ns and cs on the replication of coronavirus (cov) and on the expression of trp genes during coronavirus infection, hela-ceacam1a (hela-epithelial carcinoembryonic antigen-related cell adhesion molecule 1a) cells were inoculated with mhv-a59 (mouse hepatitis virus-a59) at moi of 30. 1/50 dilution of the ...	2014	24413991
[estimation of the tn and som contents in soil from gan nan navel orange plant area by nir diffuse spectroscopy].	the soil sampled from gan nan navel orange plant area was selected as research object, and the feasibility of analyzing the total nitrogen (tn) and soil organic matter (som) of soil was investigated by near infrared spectroscopy (nir) techniques in the wavelength range of 4 000 - 7 500 cm(-1). different pretreatment methods including multiplicative scatter correction (msc), first derivative(1st d), second derivative (2nd d), savitzkv-golay (sg), standard normalized variate (snv) and baseline wer ...	2013	24409716
extraction process optimization of polyphenols from indian citrus sinensis - as novel antiglycative agents in the management of diabetes mellitus.	diabetes mellitus is a chronic metabolic disorder characterized by increased blood glucose level. it has become an epidemic disease in the 21st century where, india leads the world with largest number of diabetic subjects. non-enzymatic glycosylation (glycation) is severe form of diabetes, occurs between reducing sugar and proteins which results in the formation of advanced glycation end products (ages) that leads to the other complicated secondary disorders. in this context, mangifera indica (m ...	2014	24397983
temporal and spatial scaling of the genetic structure of a vector-borne plant pathogen.	the ecology of plant pathogens of perennial crops is affected by the long-lived nature of their immobile hosts. in addition, changes to the genetic structure of pathogen populations may affect disease epidemiology and management practices; examples include local adaptation of more fit genotypes or introduction of novel genotypes from geographically distant areas via human movement of infected plant material or insect vectors. we studied the genetic structure of xylella fastidiosa populations cau ...	2014	24397266
[new directions of research related to chronic wound healing].	optimal nutrition, immunological state and psychological condition play an important role in the process of chronic wound healing. infections caused by pathogens resistant to commonly used antibiotics additionally complicate and disturb regeneration of wounds. as part of the treatment, modern wound dressings are used, for example designed on the basis of alginates, dextranomers, hydrogels, hydrofiber, polyurethanes foams, hydrocolloids and liquids for wound debridement such us 0.9% nacl, the pwe ...	2013	24377187
in vitro photodynamic inactivation of plant-pathogenic fungi colletotrichum acutatum and colletotrichum gloeosporioides with novel phenothiazinium photosensitizers.	the increasing tolerance to currently used fungicides in both clinical and agricultural areas is of great concern. the nonconventional light-based approach of antimicrobial photodynamic treatment (apdt) is a promising alternative to conventional fungicides. we evaluated the effects of apdt with four phenothiazinium derivatives (methylene blue [mb], new methylene blue n [nmbn], toluidine blue o [tbo], and the novel pentacyclic phenothiazinium photosensitizer [ps] s137) on conidia of three fungal ...	2014	24362436
structural characterization of the thermally tolerant pectin methylesterase purified from citrus sinensis fruit and its gene sequence.	despite the longstanding importance of the thermally tolerant pectin methylesterase (tt-pme) activity in citrus juice processing and product quality, the unequivocal identification of the protein and its corresponding gene has remained elusive. tt-pme was purified from sweet orange [ citrus sinensis (l.) osbeck] finisher pulp (8.0 mg/1.3 kg tissue) with an improved purification scheme that provided 20-fold increased enzyme yield over previous results. structural characterization of electrophoret ...	2013	24328246
citrange fruit extracts alleviate obesity-associated metabolic disorder in high-fat diet-induced obese c57bl/6 mouse.	obesity is becoming one of the global epidemics of the 21st century. in this study, the effects of citrange (citrus sinensis × poncirus trifoliata) fruit extracts in high-fat (hf) diet-induced obesity mice were studied. female c57bl/6 mice were fed respectively a chow diet (control), an hf diet, hf diet supplemented with 1% w/w citrange peel extract (cpe) or 1% w/w citrange flesh and seed extract (cfse) for 8 weeks. our results showed that both cpe and cfse regulated the glucose metabolic disord ...	2013	24317433
first evidence of c- and o-glycosyl flavone in blood orange (citrus sinensis (l.) osbeck) juice and their influence on antioxidant properties.	rp-lc-dad-esi-ms-ms separation/identification protocol has been employed for the identification and characterisation of nine c- and o-glycosyl flavonoids in moro (citrus sinensis (l.) osbeck) juice grown in southern italy. for the first time we reported the presence of five c-glycosyl flavones (lucenin-2, vicenin-2, stellarin-2, lucenin-2 4'-methyl ether and scoparin), a 3-hydroxy-3-methylglutaryl glycosyl flavonol (3-hydroxy-3-methylglutaryl glycosyl quercetin) and a flavone o-glycosides (chrys ...	2014	24295703
characterization of the antioxidant properties of phenolic extracts from some citrus peels.	this study sought to determine the distribution of free and bound phenolics in some nigerian citrus peels [orange (citrus sinensis), grapefruit (citrus paradisii) and shaddock (citrus maxima)] and characterize the antioxidant properties. the free phenolics were extracted with 80% acetone, while the bound phenolics were extracted from the alkaline and acid hydrolyzed residue with ethyl acetate. free phenolic extracts had significantly higher (p < 0.05) dpph* scavenging ability than the bound phen ...	2012	24293692
structural and physiological analyses of the alkanesulphonate-binding protein (ssua) of the citrus pathogen xanthomonas citri.	the uptake of sulphur-containing compounds plays a pivotal role in the physiology of bacteria that live in aerobic soils where organosulfur compounds such as sulphonates and sulphate esters represent more than 95% of the available sulphur. until now, no information has been available on the uptake of sulphonates by bacterial plant pathogens, particularly those of the xanthomonas genus, which encompasses several pathogenic species. in the present study, we characterised the alkanesulphonate uptak ...	2013	24282519
molecular characterization of two glutathione peroxidase genes of panax ginseng and their expression analysis against environmental stresses.	glutathione peroxidases (gpxs) are a group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ros). gpx catalyzes the reduction of hydrogen peroxide (h2o2) or organic hydroperoxides to water or alcohols by reduced glutathione. the presence of gpxs in plants has been reported by several groups, but the roles of individual members of this family in a single plant species have not been studied. two gpx cdnas were isolated and characterized from the embryoge ...	2014	24269671
the lov protein of xanthomonas citri subsp. citri plays a significant role in the counteraction of plant immune responses during citrus canker.	pathogens interaction with a host plant starts a set of immune responses that result in complex changes in gene expression and plant physiology. light is an important modulator of plant defense response and recent studies have evidenced the novel influence of this environmental stimulus in the virulence of several bacterial pathogens. xanthomonas citri subsp. citri is the bacterium responsible for citrus canker disease, which affects most citrus cultivars. the ability of this bacterium to coloni ...	2013	24260514
chlorophyllase activity in chlorophyll-free citrus chromoplasts.	chromoplast fractions from mature, chlorophyll-less 'valencia' orange (citrus sinensis l. osbeck) flavedo (= the outer coloured layer of citrus peel) showed considerable chlorophyllase activity. acetone powders prepared from chromoplast fractions had 2.5× higher specific activity than those prepared from whole flavedo. exposure of mature, chlorophyll-less fruit to ethylene caused a 2.5 to 4.0 fold increase in chlorophyllase activity. juice chromoplasts showed negligible chlorophyllase activity. ...	1983	24257977
somatic hybrid plants obtained by protoplast fusion between citrus sinensis and poncirus trifoliata.	somatic hybrid plants of rutaceae were obtained by protoplast fusion between citrus sinensis osb. ('trovita' orange) and poncirus trifoliata. protoplasts isolated from embryogenic cells of c. sinensis and from leaves of p. trifoliata, and the culture of fusion products in the presence of high concentrations of sucrose were essential requirements for the selection of hybrids. green globular embryoids derived from protoplasts resulted in the regeneration of trifoliate plants. other morphological c ...	1985	24247330
uniformity of plants regenerated from orange (citrus sinensis osb.) protoplasts.	using 25 plants (protoclones) regenerated from orange (citrus sinensis osb.) protoplasts, several characters, including leaf and flower morphology, leaf oil, isozyme patterns and chromosome number, were examined. no significant variations in each character were recorded among the protoclones. uniformity observed among protoclones was identical to that of nucellar seedlings.	1987	24241449
intergeneric somatic hybrid plants of citrus sinensis cv. hamlin and poncirus trifoliata cv. flying dragon.	intergeneric somatic hybrid plants between 'hamlin' sweet orange [citrus sinensis (l.) osbeck] and 'flying dragon' trifoliate orange (poncirus trifoliata raf.) were regenerated following protoplast fusion. 'hamlin' protoplasts, isolated from an habituated embryogenic suspension culture, were fused chemically with 'flying dragon' protoplasts isolated from juvenile leaf tissue. the hybrid selection scheme was based on complementation of the regenerative ability of the 'hamlin' protoplasts with the ...	1988	24241403
plantlet regeneration from a nacl-selected salt-tolerant callus culture of shamouti orange (citrus sinensis l. osbeck).	plantlets were regenerated from a selected salt-tolerant cell line of shamouti orange (citrus sinensis l. osbeck). embryogenesis was carried out both in the presence and absence of nacl, yielding green and white globular embryos, respectively. greening could be induced subsequently and normal heart shape embryo development was obtained. plantlet formation required exposure to kinetin prior to the introduction of the root-inducing hormone naphthalene acetic acid. this system differs from the desi ...	1989	24240461
somatic hybrid plants from sexually incompatible woody species: citrus reticulata and citropsis gilletiana.	allotetraploid intergeneric somatic hybrid plants between citrus reticulata blanco cv. cleopatra mandarin and citropsis gilletiana swing. & m. kell. (common name gillet's cherry orange) were regenerated following protoplast fusion. cleopatra protoplasts were isolated from an ovule-derived embryogenic suspension culture and fused chemically with leaf-derived protoplasts of citropsis gilletiana. cleopatra mandarin and somatic hybrid plants were regenerated via somatic embryogenesis. hybrid plant i ...	1990	24232779
cryopreservation of nucellar cells of navel orange (citrus sinensis osb. var. brasiliensis tanaka) by vitrification.	the nucellar cells of navel orange(citrus sinensis osb. var. brasiliensis tanaka) were successfully cryopreserved by vitrification. in this method, cells were sufficiently dehydrated with highly concentrated cryoprotective solution(pvs2) prior to direct plunge in liquid nitrogen. the pvs2 contains(w/v) 30% glycerol, 15% ethylene glycol and 15% dmso in murashige-tucker medium(mt) containing 0.15 m sucrose. cells were treated with 60% pvs2 at 25°c for 5 min and then chilled pvs2 at 0°c for 3 min. ...	1990	24226373
somatic hybridization in citrus: navel orange (c. sinensis osb.) and grapefruit (c. paradisi macf.).	protoplasts of navel orange, isolated from embryogenic nucellar cell suspension culture, were fused with protoplasts of grapefruit isolated from leaf tissue. the fusion products were cultured in the hormone-free medium containing 0.6 m sucrose. under the culture conditions, somatic embryogenesis of navel orange protoplasts was suppressed, while cell division of grapefruit mesophyll protoplasts was not induced. six embryoids were obtained and three lines regenerated to complete plants through emb ...	1989	24225818
an hplc-ms characterization of the changes in sweet orange leaf metabolite profile following infection by the bacterial pathogen candidatus liberibacter asiaticus.	huanglongbing (hlb) presumably caused by candidatus liberibacter asiaticus (clas) threatens the commercial u.s. citrus crop of an annual value of $3 billion. the earliest shift in metabolite profiles of leaves from greenhouse-grown sweet orange trees infected with clas, and of healthy leaves, was characterized by hplc-ms concurrently with pcr testing for the presence of clas bacteria and observation of disease symptoms. twenty, 8-month-old 'valencia' and 'hamlin' trees were grafted with budwood ...	2013	24223954
fertile fruit trees obtained by somatic hybridization: navel orange (citrus sinensis) and troyer citrange (c. sinensis x poncirus trifoliata).	nucellar cell suspension protoplasts of navel orange (citrus sinsensis osb.) were chemically fused with mesophyll protoplasts of troyer citrange (c. sinensis x poncirus trifoliata) and cultured in hormone-free murashige and tucker medium containing 0.6 m sucrose. two types of plant were regenerated through embryogenesis. one type showed intermediate mono-and difoliate leaves and the other types was identical to troyer citrange. the regenerated plants with intermediate morphology were demonstrate ...	1991	24221194
induction of triploid citrus plants from endosperm calli in vitro.	triploid hybrid citrus plants were regenerated by somatic embryogenesis in vitro from endosperm derived calli. a sequence of media formulations was used to induce and support proliferation of primary callus from endosperm, to induce embryogenesis from primary callus, and to allow embryo development leading to viable plantlets. calli were induced from cellular endosperm of citrus sinensis (sweet orange), c. xparadisi (grapefruit), and c. grandis (pummelo) excised 12-14 weeks post-anthesis. induct ...	1990	24221109
comprehensive insights on how 2,4-dichlorophenoxyacetic acid retards senescence in post-harvest citrus fruits using transcriptomic and proteomic approaches.	auxin-like 2,4-dichlorophenoxyacetic acid (2,4-d), a high-efficiency anti-stalling agent for the post-harvest fresh fruit industry, has had its use restricted due to environmental concerns. however, no other substitutes for 2,4-d are available to the post-harvest industry. insights into the molecular mechanism underlying the effects of 2,4-d on fruit quality preservation will provide a theoretical basis for exploring new safe and effective anti-stalling agents. this study comprehensively analyse ...	2014	24215076
analysis of cytoplasmic genomes in somatic hybrids between navel orange (citrus sinensis osb.) and 'murcott' tangor.	somatic hybrid plants were produced by protoplast fusion of navel orange and 'murcott' tangor. hybridity of the plants was confirmed by the restriction endonuclease analysis of nuclear ribosomal dna. all of the plants (16 clones) were normal, uniform, and had the amphidiploid chromosome number of 36 (2n=2x=18 for each parent). the cpdna analysis showed that each of the 16 somatic hybrids contained either one parental chloroplast genome or the other. in all cases, the mitochondrial genomes of the ...	1991	24212853
intergeneric somatic hybridization of sexually incompatible parents: citrus sinensis and atalantia ceylanica.	protoplast fusion using polyethylene glycol (peg) was conducted to combine citrus sinensis (l.) osbeck cv. 'hamlin' sweet orange protoplasts, isolated from nucellus-derived embryogenic callus with atalantia ceylanica (arn.) oliv, leaf protoplasts. five plants regenerated from independent fusion events following protoplast culture were identified as intergeneric allotetraploid somatic hybrids of 'hamlin' sweet orange and a. ceylanica, and confirmed by isozyme analysis and chromosome number determ ...	1993	24201965
agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants.	transgenic sweet orange (citrus sinensis l. osbeck) plants have been obtained by agrobacterium tumefaciens-mediated gene transfer. an hypervirulent a. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase ii (npt ii) and ß-glucuronidase (gus) genes was cocultivated with stem segments from in vivo grown seedlings. shoots regenerated under kanamycin selection were harvested from the stem segments within 12 weeks. shoot basal portions were assayed for g ...	1995	24194307
terpene down-regulation triggers defense responses in transgenic orange leading to resistance against fungal pathogens.	terpenoid volatiles are isoprene compounds that are emitted by plants to communicate with the environment. in addition to their function in repelling herbivores and attracting carnivorous predators in green tissues, the presumed primary function of terpenoid volatiles released from mature fruits is the attraction of seed-dispersing animals. mature oranges (citrus sinensis) primarily accumulate terpenes in peel oil glands, with d-limonene accounting for approximately 97% of the total volatile ter ...	2013	24192451
pyrolysis-gas chromatographic method for polybutene residues on navel oranges.		1968	24190082
green fluorescent protein: an in vivo reporter of plant gene expression.	protoplasts were isolated from h89, an embryogenic sweet orange (citrus sinensis (l.) osbeck cv. hamlin) suspension culture, and electroporated with p35s-gfp, a plasmid carrying the gene for the green fluorescent protein (gfp) from the bioluminescent jellyfish aequorea victoria. p35s-gfp was constructed by replacing the gus coding sequence of pbi221 with a functional gfp gene, thereby placing the gfp gene under the control of the camv 35s promoter. protoplasts were viewed by incident-light fluor ...	1995	24185445
further evidence of a cybridization requirement for plant regeneration from citrus leaf protoplasts following somatic fusion.	somatic hybridization experiments in citrus that involve the fusion of protoplasts of one parent isolated from either nucellus-derived embryogenic callus or suspension cultures with leaf-derived protoplasts of a second parent, often result in the regeneration of diploid plants that phenotypically resemble the leaf parent. in this study, plants of this type regenerated following somatic fusions of the following three parental combinations were analyzed to determine their genetic origin (nuclear a ...	1996	24178608
mining of haplotype-based expressed sequence tag single nucleotide polymorphisms in citrus.	single nucleotide polymorphisms (snps), the most abundant variations in a genome, have been widely used in various studies. detection and characterization of citrus haplotype-based expressed sequence tag (est) snps will greatly facilitate further utilization of these gene-based resources.	2013	24175923
genetic diversity in the orange subfamily aurantioideae. i. intraspecies and intragenus genetic variability.	despite the great economic importance of citrus, its phylogeny and taxonomy remain a matter of controversy. moreover pathogens of increased virulence and dramatic environmental changes are currently spreading or emerging. the objectives of the present paper, measuring genetic variability and studying its pattern of distribution, are crucial steps to optimize sampling strategies in the search of genotypes that tolerate or resist these threatening factors within the huge array of citrus and citrus ...	1996	24166329
detection of mandarin in orange juice by single-nucleotide polymorphism qpcr assay.	a dual-probe real time pcr (qpcr) dna-based analysis was devised for the identification of mandarin in orange juice. a single nucleotide polymorphism at the trnl-trnf intergenic region of the chloroplast chromosome was confirmed in nine orange (citrus sinensis) and thirteen commercial varieties of mandarin, including citrus reticulata and citrus unshiu species and a mandarin × tangelo hybrid. two short minor-groove binding fluorescent probes targeting the polymorphic sequence were used in the du ...	2014	24128588
coumarin and furanocoumarin quantitation in citrus peel via ultraperformance liquid chromatography coupled with mass spectrometry (uplc-ms).	coumarins and furanocoumarins are secondary metabolites commonly found in citrus plants. these molecules are allelochemical compounds in plants that have controversial effects on humans, such as phototoxicity and the commonly described interactions with drugs, referred to as the "grapefruit juice effect". thus, it is important to develop a reliable method to identify and quantitate the coumarins and furanocoumarins in citrus extracts. for this purpose, we herein describe an ultraperformance liqu ...	2013	24117278
rna-seq analysis of citrus reticulata in the early stages of xylella fastidiosa infection reveals auxin-related genes as a defense response.	citrus variegated chlorosis (cvc), caused by xylella fastidiosa, is one the most important citrus diseases, and affects all varieties of sweet orange (citrus sinensis l. osb). on the other hand, among the citrus genus there are different sources of resistance against x. fastidiosa. for these species identifying these defense genes could be an important step towards obtaining sweet orange resistant varieties through breeding or genetic engineering. to assess these genes we made use of mandarin (c ...	2013	24090429
identification of transcription factors potentially involved in the juvenile to adult phase transition in citrus.	the juvenile to adult transition (jat) in higher plants is required for them to reach reproductive competence. however, it is a poorly understood process in woody plants, where only a few genes have been definitely identified as being involved in this transition. this work aims at increasing our understanding of the mechanisms regulating the jat in citrus.	2013	24052558
leaf cdna-aflp analysis of two citrus species differing in manganese tolerance in response to long-term manganese-toxicity.	very little is known about manganese (mn)-toxicity-responsive genes in citrus plants. seedlings of 'xuegan' (citrus sinensis) and 'sour pummelo' (citrus grandis) were irrigated for 17 weeks with nutrient solution containing 2 μm (control) or 600 μm (mn-toxicity) mnso₄. the objectives of this study were to understand the mechanisms of citrus mn-tolerance and to identify differentially expressed genes, which might be involved in mn-tolerance.	2013	24034812
metabolic engineering of β-carotene in orange fruit increases its in vivo antioxidant properties.	orange is a major crop and an important source of health-promoting bioactive compounds. increasing the levels of specific antioxidants in orange fruit through metabolic engineering could strengthen the fruit's health benefits. in this work, we have afforded enhancing the β-carotene content of orange fruit through blocking by rna interference the expression of an endogenous β-carotene hydroxylase gene (csβ-chx) that is involved in the conversion of β-carotene into xanthophylls. additionally, we h ...	2014	24034339
citric acid production from orange peel wastes by solid-state fermentation.	valencia orange (citrus sinensis) peel was employed in this work as raw material for the production of citric acid (ca) by solid-state fermentation (ssf) of aspergillus niger cect-2090 (atcc 9142, nrrl 599) in erlenmeyer flasks. to investigate the effects of the main operating variables, the inoculum concentration was varied in the range 0.5·10(3) to 0.7·10(8) spores/g dry orange peel, the bed loading from 1.0 to 4.8 g of dry orange peel (corresponding to 35-80 % of the total volume), and the mo ...	2011	24031646
[spider diversity in cultures of citrus sinensis (rutaceae) in corrientes province, argentina].	spiders are predators that reduce insect pest populations in agroecosystems. in spite that the presence of spider assemblages has been described in different crop plants, no reports have been done for citrus species in argentina. we studied the spider community associated with cultures of citrus sinensis in the province of corrientes, argentina, in two plots (am1 irrigated and am2 unirrigated) of 0.82 hectares each. spiders were collected monthly by using pitfall traps, hand capture, agitation o ...	2013	24027921
n-acetylcysteine in agriculture, a novel use for an old molecule: focus on controlling the plant-pathogen xylella fastidiosa.	xylella fastidiosa is a plant pathogen bacterium that causes diseases in many different crops. in citrus, it causes citrus variegated chlorosis (cvc). the mechanism of pathogenicity of this bacterium is associated with its capacity to colonize and form a biofilm in the xylem vessels of host plants, and there is not yet any method to directly reduce populations of this pathogen in the field. in this study, we investigated the inhibitory effect of n-acetylcysteine (nac), a cysteine analogue used m ...	2013	24009716
modification of valencene by bio- and chemical transformation.	highly efficient production is discussed of nootkatone, the most important fragrant component of grapefruit and which possesses anti-obesity activity, from valencene, obtained from valencia orange, by the green alga, chlorella, and the fungi, mucor, botryospaeria and botryodiplodia. the microorganisms introduce an oxygen atom at an allylic position to give secondary hydroxyl and keto groups. the presented methods are a cheap one step reaction, non-hazardous and very useful for the production of ...	2013	23980411
citrus nobiletin suppresses inducible nitric oxide synthase gene expression in interleukin-1β-treated hepatocytes.	nobiletin is a polymethoxylated flavone that is abundant in the peels of citrus fruits, such as citrus unshiu (satsuma mandarin) and citrus sinensis. the dried peels of c. unshiu (chinpi) have been included in several formulae of japanese kampo medicines. nobiletin may suppress the induction of inducible nitric oxide synthase (inos), which synthesizes the inflammatory mediator nitric oxide (no) in hepatocytes.	2013	23958298
species composition and abundance of brevipalpus spp. on different citrus species in mexican orchards.	we studied the abundance of brevipalpus spp. in citrus orchards in the mexican states of yucatan, quintana roo and campeche. mites were collected from 100 trees containing a mixture of citrus species where sweet orange was always the main species. eight collections were made at each location from february 2010 to february 2011. mites from the genus brevipalpus were separated from other mites surveyed and their abundance and relationships with the different citrus species were quantified througho ...	2013	23949863
first record of anastrepha serpentina (wiedemann) (diptera: tephritidae) in citrus in brazil.	anastrepha serpentina (wiedemann) is recorded for the first time in citrus (rutaceae) in brazil. specimens were obtained from sweet orange (citrus sinensis) sampled in the municipalities of belém and capitão poço, and from mandarin orange (citrus reticulata) from tomé-açu, state of pará, brazil.	2013	23939279
first report of maconellicoccus hirsutus (green, 1908) (hemiptera: coccoidea: pseudococcidae) and the associated parasitoid anagyrus kamali moursi, 1948 (hymenoptera: encyrtidae), in brazil.	the pink hibiscus mealybug (phm), maconellicoccus hirsutus (green) (hemiptera: pseudococcidae) and the associated hymenopterous parasitoid, anagyrus kamali moursi, 1948 (hymenoptera: encyrtidae), are reported for the first time in brazil. specimens of the phm were collected on nine hosts plants, annona muricata l. (anonnaceae), glycine max (l.) merr. (fabaceae), centrolobium paraensis tul. (fabaceae), inga edulis mart. (fabaceae), hibiscus rosa-sinensis l. (malvaceae), psidium guajava l. (myrtac ...	2013	23917571
anticholinesterase and antioxidative properties of water-extractable phytochemicals from some citrus peels.	aqueous extracts from citrus peels are used in many rural communities in nigeria in treating various degenerative conditions, although the scientific basis for its use has not been well established. this study sought to investigate the anticholinesterase and antioxidant properties of aqueous extracts from some citrus peels [orange (citrus sinensis), grapefruit (citrus paradisii), and shaddock (citrus maxima)].	2014	23907423
citrus maf1, a repressor of rna polymerase iii, binds the xanthomonas citri canker elicitor ptha4 and suppresses citrus canker development.	transcription activator-like (tal) effectors from xanthomonas species pathogens act as transcription factors in plant cells; however, how tal effectors activate host transcription is unknown. we found previously that tal effectors of the citrus canker pathogen xanthomonas citri, known as pthas, bind the carboxyl-terminal domain of the sweet orange (citrus sinensis) rna polymerase ii (pol ii) and inhibit the activity of cscyp, a cyclophilin associated with the carboxyl-terminal domain of the citr ...	2013	23898043
transgenic sweet orange plants expressing a dermaseptin coding sequence show reduced symptoms of citrus canker disease.	citrus canker provoked by xanthomonas axonopodis pv. citri is a bacterial disease causing severe losses in all citrus-producing areas around the world. xanthomonas infection is considered as an endemic disease in northeast and northwest argentina, affecting as much as 10% of commercial citrus plantations. there is not known natural resistance neither in orange varieties nor in rootstocks used for grafting of commercial cultivars. to introduce resistance to this disease, plants of pineapple sweet ...	2013	23896218
the off-line combination of high performance liquid chromatography and comprehensive two-dimensional gas chromatography-mass spectrometry: a powerful approach for highly detailed essential oil analysis.	the present contribution is focused on the off-line combination of high performance liquid chromatography (hplc) and comprehensive two-dimensional gas chromatography-quadrupole mass spectrometry (gc×gc-quadms), and its application to the detailed qualitative analysis of essential oils. specifically, a silica column was exploited for the separation of the essential oil constituents in two groups, namely hydrocarbon and oxygenated compounds. after, each hplc-fraction was reduced in volume, and the ...	2013	23890548
the role of 1-deoxy-d-xylulose-5-phosphate synthase and phytoene synthase gene family in citrus carotenoid accumulation.	three 1-deoxy-d-xylulose-5-phosphate synthases (dxs) and three phytoene synthases (psy) were identified in citrus, from affymetrix genechip citrus genome array, genbank and public orange genome databases. tissue-specific expression analysis of these genes was carried out on fruit peel and flesh, flower and leaf of satsuma mandarin (citrus unshiu marc.) in order to determine their roles in carotenoid accumulation in different tissues. expression of citdxs1 and citpsy1 was highest in all test tiss ...	2013	23883976
a direct sensitivity comparison between flow-modulated comprehensive 2d and 1d gc in untargeted and targeted ms-based experiments.	the present contribution is focused on the measurement of the analytical sensitivity attained in untargeted/targeted ms/ms experiments, performed using flow-modulator comprehensive 2d and 1d gc. the comprehensive 2d experiment was performed by diverting part of the high flow (circa 80%) to flush the accumulation loop (about 28 ml/min) to waste, to reduce the gas flow entering the ion source. 1d analyses were performed through: (i) unmodulated and (ii) single column applications. an equivalent te ...	2013	23868497
culture and serological detection of the xylem-limited bacterium causing citrus variegated chlorosis and its identification as a strain ofxylella fastidiosa.	a xylem-limited bacterium resemblingxylella fastidiosa has been shown previously by electron mmcroscopy to be associated with citrus variegated chlorosis (cvc), a new disease of sweet organe tress in brazil. a bacterium was consistently cultured from plant tissues from cvc twigs of sweet orange trees but not from tissues of healthy trees on several cell-free media known to support the growth ofxylella fastidiosa. bacterial colonies typical ofx. fastidiosa became visible on pw, cs20, and pd2 agar ...	1993	23835746
interplay between aba and phospholipases a(2) and d in the response of citrus fruit to postharvest dehydration.	the interplay between abscisic acid (aba) and phospholipases a2 and d (pla2 and pld) in the response of citrus fruit to water stress was investigated during postharvest by using an aba-deficient mutant from 'navelate' orange named 'pinalate'. fruit from both varieties harvested at two different maturation stages (mature-green and full-mature) were subjected to prolonged water loss inducing stem-end rind breakdown (serb) in full-mature fruit. treatment with pla2 inhibitor aristolochic acid (at) a ...	2013	23800664
draft genome sequence of methylobacterium mesophilicum strain sr1.6/6, isolated from citrus sinensis.	methylobacterium mesophilicum strain sr1.6/6 is an endophytic bacterium isolated from a surface-sterilized citrus sinensis branch. ecological and biotechnological aspects of this bacterium, such as the genes involved in its association with the host plant and the primary oxidation of methanol, were annotated in the draft genome.	2013	23788544
phosphoproteomic analysis of chromoplasts from sweet orange during fruit ripening.	like other types of plastids, chromoplasts have essential biosynthetic and metabolic activities which may be regulated via post-translational modifications, such as phosphorylation, of their resident proteins. we here report a proteome-wide mapping of in vivo phosphorylation sites in chromoplast-enriched samples prepared from sweet orange [citrus sinensis (l.) osbeck] at different ripening stages by titanium dioxide-based affinity chromatography for phosphoprotein enrichment with lc-ms/ms. a tot ...	2014	23786612
host susceptibility of citrus cultivars to queensland fruit fly (diptera: tephritidae).	citrus crops are considered to be relatively poor hosts for queensland fruit fly, bactrocera tryoni (froggatt), as for other tephritid species. australian citrus growers and crop consultants have reported observable differences in susceptibility of different citrus cultivars under commercial growing conditions. in this study we conducted laboratory tests and field surveys to determine susceptibility to b. tryoni of six citrus cultivars [(eureka lemon (citrus limon (l.) osbeck); navel and valenci ...	2013	23786078
increased resistance against citrus canker mediated by a citrus mitogen-activated protein kinase.	mitogen-activated protein kinases (mapk) play crucial roles in plant immunity. we previously identified a citrus mapk (csmapk1) as a differentially expressed protein in response to infection by xanthomonas aurantifolii, a bacterium that causes citrus canker in mexican lime but a hypersensitive reaction in sweet oranges. here, we confirm that, in sweet orange, csmapk1 is rapidly and preferentially induced by x. aurantifolii relative to xanthomonas citri. to investigate the role of csmapk1 in citr ...	2013	23777433
digital gene expression analysis of corky split vein caused by boron deficiency in 'newhall' navel orange (citrus sinensis osbeck) for selecting differentially expressed genes related to vascular hypertrophy.	corky split vein caused by boron (b) deficiency in 'newhall' navel orange was studied in the present research. the boron-deficient citrus exhibited a symptom of corky split vein in mature leaves. morphologic and anatomical surveys at four representative phases of corky split veins showed that the symptom was the result of vascular hypertrophy. digital gene expression (dge) analysis was performed based on the illumina hiseq™ 2000 platform, which was applied to analyze the gene expression profilin ...	2013	23755275
green synthesized silver nanoparticles for selective colorimetric sensing of hg2+ in aqueous solution at wide ph range.	in the present study, the syntheses of crystalline silver and gold nanoparticles (nps) has been demonstrated in a green and environmentally friendly approach using citrus fruit extracts (lemon, citrus limon (cl-1) and sweet orange, citrus limetta (cl-2)). in addition, potentially hazardous metal ion sensing properties of these nps in aqueous solution has been explored. cl-1 and cl-2 that predominantly contained citric and ascorbic acid, exhibited different reducing abilities towards silver and g ...	2013	23741735
genome-wide characterization and expression analysis of genetic variants in sweet orange.	heterozyosity is an important feature of many plant genomes, and is related to heterosis. sweet orange, a highly heterozygous species, is thought to have originated from an inter-species hybrid between pummelo and mandarin. to investigate the heterozygosity of the sweet orange genome and examine how this heterozygosity affects gene expression, we characterized the genome of valencia orange for single nucleotide variations (snvs), small insertions and deletions (indels) and structural variations ...	2013	23738603
red orange: experimental models and epidemiological evidence of its benefits on human health.	in recent years, there has been increasing public interest in plant antioxidants, thanks to the potential anticarcinogenic and cardioprotective actions mediated by their biochemical properties. the red (or blood) orange (citrus sinensis (l.) osbeck) is a pigmented sweet orange variety typical of eastern sicily (southern italy), california, and spain. in this paper, we discuss the main health-related properties of the red orange that include anticancer, anti-inflammatory, and cardiovascular prote ...	2013	23738032
the in vitro antimicrobial activity of lavandula angustifolia essential oil in combination with other aroma-therapeutic oils.	the antimicrobial activity of lavandula angustifolia essential oil was assessed in combination with 45 other oils to establish possible interactive properties. the composition of the selected essential oils was confirmed using gc-ms with a flame ionization detector. the microdilution minimum inhibitory concentration (mic) assay was undertaken, whereby the fractional inhibitory concentration (σfic) was calculated for the oil combinations. when lavender oil was assayed in 1 : 1 ratios with other o ...	2013	23737850
a redox 2-cys mechanism regulates the catalytic activity of divergent cyclophilins.	the citrus (citrus sinensis) cyclophilin cscyp is a target of the xanthomonas citri transcription activator-like effector ptha, required to elicit cankers on citrus. cscyp binds the citrus thioredoxin cstdx and the carboxyl-terminal domain of rna polymerase ii and is a divergent cyclophilin that carries the additional loop ksgkplh, invariable cysteine (cys) residues cys-40 and cys-168, and the conserved glutamate (glu) glu-83. despite the suggested roles in atp and metal binding, the functions o ...	2013	23709667
effects of citrus sinensis juice on blood pressure.	citrus sinensis juice (csj) is a rich source of dietary flavonoids which reduce the risk of adverse cardiovascular events. this study aimed to examine the effects of four-week intake of natural and commercial orange (citrus sinensis) juice on blood pressure in healthy volunteers.	2013	23696766
itraq protein profile analysis of citrus sinensis roots in response to long-term boron-deficiency.	seedlings of citrus sinensis were fertilized with boron (b)-deficient (0μm h3bo3) or -sufficient (10μm h3bo3) nutrient solution for 15weeks. thereafter, itraq analysis was employed to compare the abundances of proteins from b-deficient and -sufficient roots. in b-deficient roots, 164 up-regulated and 225 down-regulated proteins were identified. these proteins were grouped into the following functional categories: protein metabolism, nucleic acid metabolism, stress responses, carbohydrate and ene ...	2013	23628855
tropospheric ozone reduces carbon assimilation in trees: estimates from analysis of continuous flux measurements.	high ground-level ozone concentrations are typical of mediterranean climates. plant exposure to this oxidant is known to reduce carbon assimilation. ozone damage has been traditionally measured through manipulative experiments that do not consider long-term exposure and propagate large uncertainty by up-scaling leaf-level observations to ecosystem-level interpretations. we analyzed long-term continuous measurements (>9 site-years at 30 min resolution) of environmental and eco-physiological param ...	2013	23589473
candidatus liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype.	citrus huanglongbing (hlb) disease is caused by endogenous, phloem-restricted, gram negative, uncultured bacteria named candidatus liberibacter africanus (calaf), ca. l. asiaticus (calas), and ca. l. americanus (calam), depending on the continent where the bacteria were first detected. the asian citrus psyllid vector, diaphorina citri, transmits calas and calam and both liberibacter species are present in brazil. several studies of the transcriptional response of citrus plants manifesting hlb sy ...	2013	23586643
citrus biotechnology: achievements, limitations and future directions.	citrus is one of the most important commercial and nutritional fruit crops in the world, hence it needs to be improved to cater to the diverse needs of consumers and crop breeders. genetic manipulation through conventional techniques in this genus is invariably a difficult task for plant breeders as it poses various biological limitations comprising long juvenile period, high heterozygosity, sexual incompatibility, nucellar polyembryony and large plant size that greatly hinder cultivar improveme ...	2009	23572908
development of a small scale orange juice extractor.	a small scale motorized orange juice extractor was designed and fabricated, using locally-available construction materials. the essential components of the machine include feeding hopper, top cover, worm shaft, juice sieve, juice collector, waste outlet, transmission belt, main frame, pulleys and bearings. in operation, the worm shaft conveys, crushes, presses and squeezes the fruit to extract the juice. the juice extracted is filtered through the juice sieve into juice collector while the resid ...	2010	23572610
larvicidal and irritant activities of hexane leaf extracts of citrus sinensis against dengue vector aedes aegypti l.	to assess the larvicidal and irritant activities of the hexane extracts of leaves of citrus sinensis (c. sinensis) against the early fourth instars and female adults of aedes aegypti (ae. aegypti).	0	23569887