Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| detection of mycoplasma hyopneumoniae in lung and nasal swab samples from pigs by nested pcr and culture methods. | we examined nasal swab and lung homogenate samples collected from pigs experimentally and naturally infected with mycoplasma hyopneumoniae for the detection of m. hyopneumoniae by the nested pcr (npcr) and culture methods. in the 23 experimentally infected pigs, m. hyopneumoniae was commonly detected in nasal swabs by the npcr and culture methods at 4 weeks after inoculation, and there was a significant correlation (p<0.01) between the titers of viable organisms in nasal swabs and in lung homoge ... | 2005 | 16141667 |
| comparative efficacy of two single-dose bacterins in the control of mycoplasma hyopneumoniae in swine raised under commercial conditions in brazil. | a field study was conducted in brazil to evaluate the efficacy of single vaccination of pigs with two bacterins to prevent mycoplasma hyopneumoniae lung lesions. the first (t1) treatment group (174 pigs) was injected with 2 ml of saline solution; group t2 (177 pigs) with 2 ml of bacterin a, and group t3 (174 pigs) with 2 ml of bacterin b. on days-on-test (dot) 0, 35, 66, 97 and 125, blood samples and tonsil swabs were collected from selected pigs for antibody determination (indirect elisa) and p ... | 2006 | 16129636 |
| [vaccination of sows against mycoplasma hyopneumoniae with hyoresp]. | the aim of the study was to investigate the serological reactions of pregnant sows to vaccination with hyoresp. further investigations were performed in the offspring of these sows to follow the dynamics of maternal antibodies and the reaction to vaccination at different points in time. the study was conducted in three farrow-to-finish herds endemically infected with m. hyopneumoniae. a total of 30 gilts and 31 sows were vaccinated 8 and 4 weeks ante partum with hyoresp (merial gmbh) or given ph ... | 2005 | 16124699 |
| occurrence of salmonella serotype typhimurium dt104 on a commercial swine farm before, during, and after depopulation and repopulation. | to determine whether depopulation-repopulation could be used to eradicate salmonella serotype typhimurium dt104 from a commercial swine farm in the midwestern united states. | 2005 | 16121615 |
| efficacy of tulathromycin injectable solution for the treatment of naturally occurring swine respiratory disease. | tulathromycin, a novel triamilide antimicrobial, was evaluated for treatment of swine respiratory disease (srd) in field efficacy studies involving 720 pigs in six north american swine herds. in each study, feeder pigs with clinical srd were randomly assigned in equal numbers to a group treated with tulathromycin given as a single injection at 2.5 mg/kg of body weight or to a saline-treated control group. four of the studies included a third group treated with ceftiofur sodium for 3 consecutive ... | 2005 | 16094568 |
| evaluation of the therapeutic activity of tulathromycin against swine respiratory disease on farms in europe. | the clinical efficacy of tulathromycin in the treatment of natural outbreaks of swine respiratory disease (srd) was evaluated at five european sites. pigs (1 to 6 months of age) exhibiting clinical signs of srd were treated intramuscularly with tulathromycin (n = 247) at 2.5 mg/kg on day 0 versus either tiamulin (n = 102) at 15 mg/kg on days 0, 1, and 2 (germany, the netherlands, and the united kingdom) or florfenicol (n = 20) at 15 mg/kg on days 0 and 2 (france). actinobacillus pleuropneumoniae ... | 2005 | 16094567 |
| evaluation of tulathromycin for the treatment of pneumonia following experimental infection of swine with mycoplasma hyopneumoniae. | tulathromycin was evaluated in the treatment of pneumonia in weaned pigs inoculated intranasally with mycoplasma hyopneumoniae. five days postchallenge, the pigs were randomized to treatment with a single im administration of saline, a single im administration of tulathromycin (2.5 mg/kg; day 0), or three im administrations of enrofloxacin (5.0 mg/kg; days 0, 1, 2). pigs were necropsied on day 12 or 13. unchallenged controls remained healthy with no lung pathology. compared with saline, coughing ... | 2005 | 16094566 |
| swine and poultry pathogens: the complete genome sequences of two strains of mycoplasma hyopneumoniae and a strain of mycoplasma synoviae. | this work reports the results of analyses of three complete mycoplasma genomes, a pathogenic (7448) and a nonpathogenic (j) strain of the swine pathogen mycoplasma hyopneumoniae and a strain of the avian pathogen mycoplasma synoviae; the genome sizes of the three strains were 920,079 bp, 897,405 bp, and 799,476 bp, respectively. these genomes were compared with other sequenced mycoplasma genomes reported in the literature to examine several aspects of mycoplasma evolution. strain-specific region ... | 2005 | 16077101 |
| epitope mapping of mycoplasma hyopneumoniae using phage displayed peptide libraries and the immune responses of the selected phagotopes. | phage display techniques have been widely employed to map the epitope structures which served as the basis for developing molecular vaccines. in the present study, we applied this technique to map the epitopes of mycoplasma hyopneumoniae, the etiologic agent causing swine enzootic pneumonia, and evaluated directly the immune responses in mice of the selected phage-displayed epitopes (phagotopes). two phage-displayed random peptide libraries were biopanned with the protein a-purified igg of the r ... | 2005 | 16054642 |
| the effect of polyclonal antibody on intracellular calcium increase induced by mycoplasma hyopneumoniae in porcine tracheal cells. | an investigation was undertaken to assess whether polyclonal convalescent and hyperimmune sera obtained from pigs inhibit mycoplasma hyopneumoniae induced increases in intracellular calcium [ca2+](i) in ciliated porcine tracheal cells. basal [ca2+](i) in the tracheal cells was 97+/-13 nm (n=22 cells in four experiments) and after exposure to m. hyopneumoniae (300 micro g/ml or 10(11) ccu/ml), [ca2+](i) increased by 246+/-56 nm within 100 s. after pre-treatment with hyperimmune or convalescent se ... | 2006 | 16051507 |
| evaluation of the aerosol transmission of a mixed infection of mycoplasma hyopneumoniae and porcine reproductive and respiratory syndrome virus. | to evaluate the transmission of mycoplasma hyopneumoniae and porcine reproductive and respiratory syndrome virus (prrsv) by aerosol as either a single or mixed infection, 28 pigs were inoculated intratracheally with m hyopneumoniae on day 0 and infected intranasally with prrsv on day 35; they were housed together in a barn. to assess the aerosol transmission of m hyopneumoniae as a single infection, one trailer (a) containing 10 five-week-old sentinel pigs was placed along the south side of the ... | 2005 | 16040942 |
| development of nested polymerase chain reaction for the detection of mycoplasma hyopneumoniae in formalin-fixed paraffin-embedded lung tissue. | 2005 | 16035187 | |
| the occurrence of mycoplasmas in the lungs of pigs in new zealand. | attempts were made to recover and serologically identify mycoplasmas from the lungs of 50 pigs with mycoplasmal pneumonia and from 50 lungs without gross evidence of pneumonia. mycoplasma hyopneumoniae and m. hyorhinis were respectively cultured from 30% and 50% of pneumonic lungs and the former species was also recovered from 12% of non-pneumonic lungs. three other isolates (one from pneumonic and two from non-pneumonic lungs) differed in colonial morphology from m. hyopneumoniae and m. hyorhin ... | 1985 | 16031118 |
| diagnosis of mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae, the cause of enzootic pneumonia, remains an important pathogen in the swine industry. this small, complex organism colonizes the ciliated cells of the respiratory tract, resulting in little exposure to the immune system. confirming the presence of m. hyopneumoniae, as well as identifying its role in respiratory disease and pneumonia, remains challenging to the veterinary profession. while culture of the organism remains the gold standard for identification, the use of s ... | 2004 | 15984347 |
| the diversity of mycoplasma hyopneumoniae within and between herds using pulsed-field gel electrophoresis. | over the years, pulsed-field gel electrophoresis (pfge) has been proven a robust technique to type isolates with a high resolution and a good reproducibility. in this study, a pfge protocol is described for the typing of mycoplasma hyopneumoniae isolates. the potential of this technique was demonstrated by comparing m. hyopneumoniae isolates obtained from the same as well as from different herds. the use of two different restriction enzymes, sali and apai, was evaluated. for each enzyme, the res ... | 2005 | 15963665 |
| efficacy of in-feed medication with tylosin for the treatment and control of mycoplasma hyopneumoniae infections. | the efficacy of in-feed medication with tylosin for the treatment of enzootic pneumonia was examined in an experimental mycoplasma hyopneumoniae infection model. one group of 10 conventional m. hyopneumoniae-free pigs was inoculated intratracheally with a highly virulent field isolate of m. hyopneumoniae; a second group of 10 pigs was inoculated in the same way and after 12 days was given tylosin at 100 mg/kg feed for 21 days; a third group of 10 pigs was inoculated with sterile culture medium, ... | 2005 | 15879541 |
| evaluation of mycoplasma hyopneumoniae growth by flow cytometry. | in the present study we evaluated the potential application of the flow cytometry (fc) technique to determine the growth rates of mycoplasma hyopneumoniae in a broth medium. | 2005 | 15836473 |
| enzootic pneumonia of pigs--a diagnostic dilemma. | in an enzootic pneumonia-free australian pig herd, an outbreak of a severe respiratory disease in the grow-out herd was initially diagnosed as acute tracheitis and pneumonia precipitated by the dusty environment, with a superimposed mixed infection of pasteurella multocida and arcanobacterium pyogenes. culture for mycoplasma hyopneumoniae, salmonella sp and fungi was negative. the outbreak persisted. subsequently, gross lesions consistent with enzootic pneumonia occurred, but histological lesion ... | 2005 | 15825621 |
| evidence of mycoplasma hyopneumoniae infection among slaughtered pigs in sri lanka. | 2005 | 15729894 | |
| serological diagnosis of enzootic pneumonia of swine by a double-sandwich enzyme-linked immunosorbent assay using a monoclonal antibody and recombinant antigen (p46) of mycoplasma hyopneumoniae. | to facilitate the control of enzootic pneumonia (ep) of swine caused by mycoplasma hyopneumoniae, the complement fixation (cf) test has been used for the detection of m. hyopneumoniae antibodies. however, the cf test is a cumbersome and time-consuming technique and cross-reactivity are major drawbacks associated with this method. to circumvent these drawbacks, we have developed a double-sandwich enzyme-linked immunosorbent assay (elisa), consisting of purified monoclonal antibody (mab) against t ... | 2004 | 15708823 |
| comparison of two swine mycoplasma hyopneumoniae enzyme-linked immunosorbent assays for detection of antibodies from vaccinated pigs and field serum samples. | mycoplasma hyopneumoniae (mhyo) causes mycoplasmal pneumonia, an economically important disease of swine. serodiagnosis of mhyo is based on the current available commercial enzyme immunoassays for detection of swine antibodies against mhyo, which are the indirect enzyme-linked immunosorbent assay (elisa) and the blocking elisa (b-elisa). because of the limited information available for these elisas, these 2 assays were compared by testing 347 serum samples collected from vaccinated pigs at 0, 13 ... | 2005 | 15690953 |
| assessing mycoplasma hyopneumoniae aerosol movement at several distances. | 2005 | 15689041 | |
| postweaning multisystemic wasting syndrome--pmws. the first year with the disease in sweden. | postweaning multisystemic wasting syndrome (pmws) is causally associated with porcine circovirus type 2 (pcv2) infection of pigs. pcv2 has been present in the swedish pig population for at least ten years. despite this, no signs of pmws were observed in pigs of sweden until 2003. since then the disease has spread slowly in sweden. the pig population of sweden is geographically isolated, the density of pigs and the pathogen load in the country is low and the trade of animals is organised in a res ... | 2004 | 15663213 |
| genome update: proteome comparisons. | 2005 | 15632419 | |
| establishment of swine interleukin-6 sandwich elisa. | we established a sandwich enzyme-linked immunosorbent assay (elisa) for swine interleukin-6 (swil-6), which was applied for detection of swil-6 in vitro and in vivo. anti-swil-6 rabbit- and goat-polyclonal antibodies, and monoclonal antibody (mab) were prepared, conforming that all of the antibodies were reactive with recombinant swil-6 by western blotting and indirect elisa. a sandwich elisa was developed using the mab as a capture antibody and biotinylated goat-polyclonal antibody as a detecti ... | 2005 | 15582688 |
| quantification of the spread of mycoplasma hyopneumoniae in nursery pigs using transmission experiments. | mycoplasma hyopneumoniae (m. hyopneumoniae) is present in almost all swine herds worldwide, but transmission of the pathogen through herds is not yet fully clarified. the aim of this study, performed in 2003, was to investigate and to quantify the transmission of m. hyopneumoniae under experimental conditions by means of an adjusted reproduction ratio (rn). this rn-value, calculated according to the final size method, expresses the mean number of secondary infections due to one typical infectiou ... | 2004 | 15579347 |
| the effect of antimicrobial growth promoter withdrawal on the health of weaned pigs in finland. | the use of the antimicrobial growth promoters (agps) carbadox and olaquindox has been banned in the european union (eu) since september 1999. we studied the effects of the withdrawal on the health of weaned piglets on two types of piglet-producing farms (farrowing herds and farrow-to-finish herds) from the different regions of finland. farms with no major problems with post-weaning diarrhoea were selected for the study to better evaluate the effect of agps alone. data on production, medication a ... | 2004 | 15579342 |
| a biological network describing infection with mycoplasma hyopneumoniae in swine herds. | we have made a biological model based on a bayesian network for risk factors for infection with mycoplasma hyopneumoniae (mycoplasma) in slaughter pigs; prevalence of infection is based on veterinary examinations. the model is a probabilistic/stochastic determination based on the inherent biological uncertainty of the severity of infection with mycoplasma and the derived stochastic effects on productivity for the herd. the conditional probabilities in the bayesian network are based on published ... | 2004 | 15579341 |
| blending of a conventional mycoplasma hyopneumoniae vaccine with a positive marker: tracking of immunised pigs by peptide-specific antibodies raised to the marker component. | highly immunodominant marker antigens simply blended to existing veterinary vaccines may represent a smart approach for addressing the still open issue of vaccination compliance. this approach was evaluated by blending a widely deployed mycoplasma hyopneumoniae vaccine with a peptide-klh (keyhole limpet hemocyanin) conjugate as marker. piglets were vaccinated twice with: (i) a combination of the m. hyopneumoniae-specific vaccine and the marker, (ii) m. hyopneumoniae-specific vaccine, (iii) marke ... | 2005 | 15563920 |
| experimental reproduction of postweaning multisystemic wasting syndrome in pigs by dual infection with mycoplasma hyopneumoniae and porcine circovirus type 2. | the objectives of this study were to investigate the interactions between mycoplasma hyopneumoniae and porcine circovirus type 2 (pcv2) and to establish a model for studying the pathogenesis of and testing intervention strategies for the control of pcv2-associated porcine respiratory disease complex (prdc). sixty-seven pigs were randomly assigned to four groups. group 1 (n=17) pigs served as controls, group 2 (n=17) pigs were inoculated with m. hyopneumoniae, group 3 (n=17) pigs were dual infect ... | 2004 | 15557072 |
| a system response to an outbreak of enzootic pneumonia in grow/finish pigs. | a mycoplasma hyopneumoniae-negative commercial swine production system broke with enzootic pneumonia at their grow/finish site in southern manitoba in october, 2003. system responses included feed medication, depopulation, delayed shipment of pigs to the infected site, vaccination of at risk sow herds, and disinfection when grow/finish site depopulation was completed. | 2004 | 15532888 |
| decreased protein accretion in pigs with viral and bacterial pneumonia is associated with increased myostatin expression in muscle. | chronic respiratory infections reduce growth in pigs but protein accretion (pa) during an ongoing multifactorial respiratory infection has not been determined, and the mechanisms underlying growth inhibition are largely unknown. the objectives of this study were to determine whether viral and bacterial pneumonia in young pigs decrease pa, increase serum il-1beta and il-6, and increase myostatin (mstn) mrna in biceps femoris and triceps muscles. mycoplasma hyopneumoniae (mh) or medium was given i ... | 2004 | 15514274 |
| in vitro susceptibilities of mycoplasma hyopneumoniae field isolates. | the in vitro susceptibilities of 21 mycoplasma hyopneumoniae field isolates were determined using a broth microdilution technique. one isolate showed acquired resistance to lincomycin, tilmicosin, and tylosin, while five isolates were resistant to flumequine and enrofloxacin. acquired resistance against these antimicrobials in m. hyopneumoniae field isolates was not reported previously. | 2004 | 15504886 |
| the genome sequence of mycoplasma hyopneumoniae strain 232, the agent of swine mycoplasmosis. | we present the complete genome sequence of mycoplasma hyopneumoniae, an important member of the porcine respiratory disease complex. the genome is composed of 892,758 bp and has an average g+c content of 28.6 mol%. there are 692 predicted protein coding sequences, the average protein size is 388 amino acids, and the mean coding density is 91%. functions have been assigned to 304 (44%) of the predicted protein coding sequences, while 261 (38%) of the proteins are conserved hypothetical proteins a ... | 2004 | 15489423 |
| increased production of proinflammatory cytokines following infection with porcine reproductive and respiratory syndrome virus and mycoplasma hyopneumoniae. | induction of the proinflammatory cytokines interleukin-1 (il-1) (alpha and beta), il-6, il-8, il-10, il-12, and tumor necrosis factor alpha (tnf-alpha) in pulmonary alveolar macrophages (pams) was assessed following experimental infection with porcine reproductive and respiratory syndrome virus (prrsv) and/or mycoplasma hyopneumoniae by using in vivo and in vitro models. the in vivo model consisted of pigs infected with prrsv and/or m. hyopneumoniae and necropsied at 10, 28, or 42 days postinfec ... | 2004 | 15358650 |
| characterization of specific antibodies and the establishment of sandwich elisa and elispot systems for swine il-4. | we produced four monoclonal antibodies (mab) and two polyclonal antibodies using the purified cytokine expressed in bacteria and characterized them. specific binding of each of the mab and polyclonal antibodies to recombinant swine il-4 (rswil-4) purified from escherichia coli and baculovirus was demonstrated in an indirect elisa and/or in western blotting. we established a sandwich enzyme-linked immunosorbent assay (elisa) for measuring concentration of swil-4 in biological samples and establis ... | 2004 | 15325518 |
| mycoplasma hyopneumoniae p65 surface lipoprotein is a lipolytic enzyme with a preference for shorter-chain fatty acids. | mycoplasma hyopneumoniae is the most significant bacterial pathogen of the respiratory tract of swine. p65 is an immunodominant surface lipoprotein of m. hyopneumoniae that is specifically recognized during disease. analysis of the translated amino acid sequence of the gene encoding p65 revealed similarity to the gdsl family of lipolytic enzymes. to examine the lipolytic activity of p65, the gene was cloned and expressed in escherichia coli after truncation of the prokaryotic lipoprotein signal ... | 2004 | 15317784 |
| development of two real-time pcr assays for the detection of mycoplasma hyopneumoniae in clinical samples. | in order to improve the diagnosis of enzootic pneumonia (ep) in pigs two real-time polymerase chain reaction (rtpcr) assays for the detection of mycoplasma hyopneumoniae in bronchial swabs from lung necropsies were established and validated in parallel. as a gold standard, the current "mosaic diagnosis" was taken, including epidemiological tracing, clinical signs, macro- and histopathological lesions of the lungs and immunofluorescence. one rtpcr is targeting a repeated dna element of the m. hyo ... | 2004 | 15288927 |
| association between mycoplasma hyopneumoniae at different respiratory sites and presence of histopathological lung lesions. | 2004 | 15285285 | |
| robust bayesian prediction of subject disease status and population prevalence using several similar diagnostic tests. | sometimes several diagnostic tests are performed on the same population of subjects with the aim of assessing disease status of individuals and the prevalence of the disease in the population, but no test is a reference test. although the diagnostic tests may have the same biological underpinnings, test results may disagree for some specific animals. in that case, it may be difficult to determine disease status for individual subjects, and consequently population prevalence estimation becomes di ... | 2004 | 15236427 |
| effects of mycoplasma hyopneumoniae vaccine on pigs naturally infected with m. hyopneumoniae and porcine reproductive and respiratory syndrome virus. | the effects of a single-dose mycoplasma hyopneumoniae vaccine was studied in growing pigs. each of 24 vaccinated cohorts of approximately 1200 pigs reared in separate barns was matched by time, farm site, and sex with unvaccinated cohorts. pigs were naturally exposed to m. hyopneumoniae and porcine reproductive respiratory syndrome virus (prrsv). daily weight gain was 42 g per pig per day higher and mortality rate was 15.2/1000 pigs lower for vaccinated cohorts. age at prrsv onset was associated ... | 2004 | 15149792 |
| efficacy of vaccines against bacterial diseases in swine: what can we expect? | this paper discusses what can be expected with regard to efficacy of antibacterial vaccines used in swine, based on the present knowledge of pathogen-host interactions. first, vaccination against bacteria that mainly cause disease by production of exotoxins is considered. vaccines containing the inactivated toxin or a non-toxic but antigenic recombinant protein derived from the exotoxin can be expected to provide protection against disease. the degree of protection induced by such vaccines varie ... | 2004 | 15145504 |
| phylogeny of firmicutes with special reference to mycoplasma (mollicutes) as inferred from phosphoglycerate kinase amino acid sequence data. | the phylogenetic position of the mollicutes has been re-examined by using phosphoglycerate kinase (pgk) amino acid sequences. hitherto unpublished sequences from mycoplasma mycoides subsp. mycoides, mycoplasma hyopneumoniae and spiroplasma citri were included in the analysis. phylogenetic trees based on pgk data indicated a monophyletic origin for the mollicutes within the firmicutes, whereas bacilli (firmicutes) and clostridia (firmicutes) appeared to be paraphyletic. with two exceptions, i.e. ... | 2004 | 15143038 |
| bf, hp, dqb and drb are associated with haemolytic complement activity, acute phase protein reaction and antibody response in the pig. | in order to examine the loci factor b (bf), c3, corticotropin releasing hormone (crh), dqb, drb, haptoglobin (hp) and transforming growth factor beta 1 (tgfb1) for association with traits of humoral, specific and unspecific defence f2-animals of a porcine resource family were genotyped at single nucleotide polymorphisms within these loci. haemolytic complement activity in the alternative and classical pathway, c3c and haptoglobin serum concentration and antibody titres were determined immediatel ... | 2004 | 15135987 |
| proteolytic processing of the mycoplasma hyopneumoniae cilium adhesin. | mycoplasma hyopneumoniae is an economically significant swine pathogen that colonizes the respiratory ciliated epithelial cells. cilium adherence is mediated by p97, a surface protein containing a repeating element (r1) that is responsible for binding. here, we show that the cilium adhesin is proteolytically processed on the surface. proteomic analysis of strain j proteins identified cleavage products of 22, 28, 66, and 94 kda. n-terminal sequencing showed that the 66- and 94-kda proteins posses ... | 2004 | 15102789 |
| intra-unit correlations in seroconversion to actinobacillus pleuropneumoniae and mycoplasma hyopneumoniae at different levels in danish multi-site pig production facilities. | in this paper, multilevel logistic models which take into account the multilevel structure of multi-site pig production were used to estimate the variances between pigs produced in danish multi-site pig production facilities regarding seroconversion to actinobacillus pleuropneumoniae serotype 2 (ap2) and mycoplasma hyopneumoniae (mh). based on the estimated variances, three newly described computational methods (model linearisation, simulation and linear modelling) and the standard method (laten ... | 2004 | 15099713 |
| immunohistochemical labelling of cytokines in lung lesions of pigs naturally infected with mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae (mh) is the primary agent of porcine enzootic pneumonia (pen), a chronic respiratory disease endemic to pig farms, and characterized histologically by infiltration of mononuclear cells in airways and prominent hyperplasia of the bronchus-associated lymphoid tissue (balt). to gain further insight into the pathogenesis of pen, cytokine expression in the lung, with particular attention to the balt, was examined immunohistochemically in pigs naturally infected with mh. an in ... | 2004 | 15053934 |
| experimental dual infection of pigs with an h1n1 swine influenza virus (a/sw/hok/2/81) and mycoplasma hyopneumoniae. | dual infection of pigs with swine influenza virus (siv) and mycoplasma hyopneumoniae was carried out to compare the clinical and pathological effects of dual infection in caesarian derived and colostrums deprived (cdcd) pigs, with that of a single infection with m. hyopneumoniae. in experiment 1, 40-day-old cdcd pigs were inoculated only with siv (a/sw/hok/2/81, h1n1). the virus was isolated from nasal swabs for 5-6 days. none of these pigs showed clinical signs of infection throughout the exper ... | 2004 | 15036530 |
| antibody response in sows and piglets following vaccination against mycoplasma hyopneumoniae, toxigenic pasteurella multocida, and actinobacillus pleuropneumoniae. | the aim of the experimental study was to compare the humoral immune response and occurrence of adverse effects following single or multiple simultaneous vaccination of sows against mycoplasma hyopneumonia, toxigenic pasteurella multocida, and actinobacillus pleuropneumoniae. in addition, passively transferred antibodies to piglets were studied until weaning at 3 weeks of age. fever was seen in a few sows within the first 12 hours after the 1st and 2nd vaccination. no difference in the occurrence ... | 2004 | 14979438 |
| dynamics of mycoplasma hyopneumoniae infection in 12 farms with different production systems. | this study had 2 objectives: 1) to determine the involvement of mycoplasma hyopneumoniae in respiratory outbreaks in herds of pigs, with the use of a nested polymerase chain reaction (npcr) and an enzyme-linked immunosorbent assay (elisa); and 2) to determine if the dynamics of m. hyopneumoniae infection differ between 3-site versus 1- or 2-site production systems (in which at least farrowing/gestation and nursery pigs are on the same site). animals of different ages from 12 spanish farms with r ... | 2004 | 14979430 |
| porcine circovirus-2 and concurrent infections in the field. | porcine circovirus-2 (pcv-2) is the necessary cause of post-weaning multisystemic wasting syndrome (pmws) in swine; however, a variety of co-factors, including other infectious agents, are thought to be necessary in the full expression of disease. porcine parvovirus (ppv) was found in the inoculum used in the first experiments to reproduce pmws in gnotobiotic swine. retrospective and prospective studies in the field and laboratory have demonstrated pcv-2 can act synergistically with ppv to enhan ... | 2004 | 14741128 |
| effects of gestational and lactational exposure to organochlorine compounds on cellular, humoral, and innate immunity in swine. | few studies have characterized the immunotoxic potential of complex mixtures of organochlorines (ocs) that bear environmental relevance. we monitored immune parameters in male piglets exposed in utero and through lactation to an oc mixture which was designed to approximate that found in the traditional diet of arctic aboriginal populations. prepubertal sows were administered orally either corn oil (control group) or the oc mixture in increasing doses (low, medium, and high). the sows were insemi ... | 2004 | 14704374 |
| evaluation of virulence of mycoplasma hyopneumoniae field isolates. | the course of enzootic pneumonia, caused by mycoplasma hyopneumoniae, is strongly influenced by management and housing conditions. other factors, including differences in virulence between m. hyopneumoniae strains, may also be involved. the aim of this study was to evaluate the virulence of six m. hyopneumoniae field isolates and link it to genetic differences as determined by randomly amplified polymorphic dna (rapd) analysis. ninety, conventional m. hyopneumoniae-free piglets were inoculated i ... | 2003 | 14654289 |
| the pyruvate dehydrogenase complex of mycoplasma hyopneumoniae contains a novel lipoyl domain arrangement. | the genes encoding the pyruvate dehydrogenase (pdh) complex (pdha, pdhb, pdhc and pdhd) from mycoplasma hyopneumoniae have been cloned and sequenced. the genes are arranged into two operons, designated pdhab and pdhcd, which are not found together in the chromosome. the pdha, pdhb, pdhc and pdhd genes encode proteins of predicted molecular masses of 44.2 kda (pyruvate dehydrogenase major subunit; e1alpha), 36.6 kda (pyruvate dehydrogenase minor subunit; e1beta), 33.1 kda (dihydrolipoyl acetyltra ... | 2003 | 14597175 |
| porcine tlr2 and tlr6: identification and their involvement in mycoplasma hyopneumoniae infection. | we successfully cloned and sequenced porcine toll-like receptor (tlr2) and tlr6 cdna from porcine alveolar macrophages stimulated with 10 microg/ml lipopolysaccharide (lps). the open reading frames (orfs) of the porcine tlr2 and tlr6 cdna were shown to be 2358 and 2391 bp in length and to encode 785 and 796 amino acids, respectively. the predicted amino acid sequence of porcine tlr2 was 72.3% homologous to human tlr2 and 61.0% homologous to murine tlr2. that of porcine tlr6 was 74.4% homologous ... | 2003 | 14585198 |
| interleukin-10, interleukin-12, and interferon-gamma levels in the respiratory tract following mycoplasma hyopneumoniae and prrsv infection in pigs. | the cytokine profile associated with either a t helper 1 (th1) or th2 response in a porcine respiratory disease model was assessed by measuring il-12, il-10 and ifn-gamma using rt-pcr and elisa, respectively. il-10, il-12, and ifn-gamma levels in pulmonary alveolar macrophages and bronchial lavage fluid were increased in pigs infected with porcine reproductive and respiratory syndrome virus (prrsv), mycoplasma hyopneumoniae, or both pathogens. at 10 days post-infection (dpi), both il-10 and il-1 ... | 2003 | 14583150 |
| association of porcine circovirus 2 with porcine respiratory disease complex. | a retrospective study was performed on natural cases of porcine respiratory disease complex (prdc) to determine the association and prevalence of prdc with porcine circovirus 2 (pcv2) and other co-existing pathogens in korea. histologically, alveolar septa were markedly thickened by infiltrates of mononuclear cells. moderate to marked multifocal peribronchial and peribronchiolar fibrosis were present and often extended into the airway lamina propria. among the 105 pigs with prdc, 85 were positiv ... | 2003 | 14550736 |
| evaluation of bacterial rnase p rna as a drug target. | rna has gained increasing importance as a therapeutic target. however, so far mrnas rather than stable cellular rnas have been considered in such studies. in bacteria, the trna-processing enzyme rnase p has a catalytic rna subunit. fundamental differences in structure and function between bacterial and eukaryotic rnase p, and its indispensability for cell viability make the bacterial enzyme an attractive drug target candidate. herein we describe two approaches utilized to evaluate whether the ca ... | 2003 | 14523922 |
| evaluation of antibody formation, daily weight gain and meat quality after vaccination of piglets against mycoplasma hyopneumoniae. | a mycoplasma hyopneumoniae vaccine (respisure, pfizer ah) was tested for its effects on antibody formation, daily weight gain (dwg) in different growing periods, lung lesions and quality of meat (chemical composition, physicochemical properties and fatty acid composition). two groups of conventional piglets were used for the investigation. one group of 11 females and 11 males was vaccinated intramuscularly at the age of 1 and 3 weeks. the other group of 22 piglets was left nonvaccinated as contr ... | 2003 | 14516156 |
| reduction of economic losses caused by mycoplasmal pneumonia of pigs by vaccination with respisure and by tiamutin treatment. | the possibilities and economic benefits of controlling mycoplasmal pneumonia of pigs caused by mycoplasma hyopneumoniae by immunisation with respisure and by tiamutin treatment were studied. the experiment was carried out in a herd comprising 1000 sows which was free of prrs, aujeszky's disease, swine dysentery and leptospirosis, and the prevalence of mycoplasmal pneumonia was low because the farm had recently been restocked. groups c1 and c2 served as untreated controls, while groups r1 and r2 ... | 2003 | 14516155 |
| new species: mycoplasma hyopneumoniae; a causative agent of virus pig pneumonia. | 1965 | 14323369 | |
| comparison of two commercial enzyme-linked immunosorbent assays for the detection of antibodies against mycoplasma hyopneumoniae and correlation with herd status. | 1999 | 12968742 | |
| effect of vaccination with selective bacterins on conventional pigs infected with type 2 porcine circovirus. | the objective of this study was to determine whether vaccination with bacterins commonly used in the usa, when administered at a time typical of us protocol, enhances porcine circovirus type 2 (pcv2) replication and the incidence and severity of clinical signs and lesions characteristic of postweaning multisystemic wasting syndrome (pmws) in conventional pigs. sixty-one pigs free of pcv2 were randomly assigned to four groups. groups 1 (n = 15) and 2 (n = 15) pigs served as sham-inoculated negati ... | 2003 | 12949409 |
| a morphologic and immunohistochemical study of the bronchus-associated lymphoid tissue of pigs naturally infected with mycoplasma hyopneumoniae. | porcine enzootic pneumonia (pen), caused by mycoplasma hyopneumoniae (mh), has been described in pigs in all geographic areas. the disease is characterized by high morbidity and low mortality rates in intensive swine production systems. a morphologic and immunohistochemical study was done to determine the cellular populations present in lung parenchyma of infected pigs, with special attention to the bronchus-associated lymphoid tissue (balt). polyclonal and monoclonal antibodies were used for th ... | 2003 | 12824511 |
| monoclonal antibodies to escherichia coli-expressed p46 and p65 membranous proteins for specific immunodetection of mycoplasma hyopneumoniae in lungs of infected pigs. | the p46 and p65 proteins of mycoplasma hyopneumoniae are two membranous proteins carrying species-specific antigenic determinants. based on the genomic sequence of the reference strain atcc 25934, primers were designed for pcr amplification of the genes encoding entire p46 (1,260 bp) and p65 (1,803 bp) and n-terminally truncated p65(c) (1,200 bp). these primers were shown to be specific to m. hyopneumoniae since no dna amplicons could be obtained with other mycoplasma species that commonly colon ... | 2003 | 12738649 |
| plaque reduction test: an alternative method to assess specific antibody response to piii-displayed peptide of filamentous phage m13. | phage-displayed peptide systems have been used to identify the immunogenic epitopes and to develop the design of peptide-based or peptide-displaying phages themselves as vaccine candidates. to estimate the humoral immunity of phage-based vaccine, it is necessary to evaluate the antibody response specifically directed at the displayed peptide. enzyme-linked immunosorbent assays (elisas) and western blot analysis are commonly used for this purpose. however, using these methods, it is not easy to d ... | 2003 | 12738371 |
| identification and characterization of cytopathogenic mycoplasma hyorhinis from swine farms with a history of abortions. | a virus-like cytopathic agent isolated from swine farms with a history of recurrent abortion episodes was investigated. we employed a differential display reverse transcription-polymerase chain reaction (ddrt-pcr) to obtain genetic information of the cytopathic agent. partial nucleotide sequence (527 bp) obtained from differentially displayed pcr fragments showed 88.7% similarity with the 23s rrna gene of mycoplasma hyopneumoniae. unexpectedly, the 5' portion (1-333 bp) of the sequence shared 96 ... | 2003 | 12736433 |
| enhanced murine antigen-specific gamma interferon and immunoglobulin g2a responses by using mycobacterial esat-6 sequences in dna vaccines. | the mycobacterium tuberculosis protein esat-6 has unusual immune stimulating activities, has been implicated in the recall of long-lived immunity, and induces protection against tuberculosis in mice. for many diseases caused by bacterial or viral pathogens, a strong cell-mediated immune (i.e., type 1) response is often required for recovery or protection. therefore, it is important to design immunization regimens that induce agent-specific type 1 immunity. we have shown in previous studies that ... | 2003 | 12654848 |
| infection of growing swine with porcine reproductive and respiratory syndrome virus and mycoplasma hyopneumoniae--effects on growth, serum metabolites, and insulin-like growth factor-i. | this study evaluated the influence of concomitant infections with porcine reproductive and respiratory syndrome virus (prrsv) and mycoplasma hyopneumoniae on growth performance, serum metabolite concentrations, and serum insulin-like growth factor-i (igf-i) in growing pigs. twenty-two barrows (10 weeks of age) were treated with either an intranasal administration of prrsv and an intratracheal infusion of m. hyopneumoniae (treatment; n = 8) or a sham inoculation with medium (sham; n = 8), or were ... | 2003 | 12619553 |
| expression and immunogenicity of mycoplasma hyopneumoniae heat shock protein antigen p42 by dna vaccination. | mycoplasma hyopneumoniae is the etiological agent of swine enzootic pneumonia, a chronic nonfatal disease affecting pigs of all ages. the goal of this study was to design dna vaccines by constructing plasmid pcdna3/p42, carrying the heat shock protein gene p42 of m. hyopneumoniae, and to evaluate the immune responses elicited in balb/c mice. the expression of p42 was first examined in transfected nih 3t3 cells by reverse transcription-pcr to ensure that the construct was functional. the humoral ... | 2003 | 12595427 |
| protective effects of oral microencapsulated mycoplasma hyopneumoniae vaccine prepared by co-spray drying method. | the efficacy of mycoplasma hyopneumoniae oral vaccine was investigated in microsphere dosage form. a co-spray drying process was used to apply an encapsulating material, eudragit l30 d-55, to microspheres containing mycoplasma hyopneumoniae antigens. the microspheres were generally effective (>93%) with protein release at ph 7.4, but almost none were released at ph 1.2, for 3 hr in an in vitro dissolution test. an spf-swine model was used to evaluate the effectiveness of the microspheres as an o ... | 2003 | 12576707 |
| incidence of reinfections with mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae in pig farms located in respiratory-disease-free regions of switzerland--identification and quantification of risk factors. | the objective of the study was to identify risk factors for reintroduction of actinobacillus pleuopneumoniae and mycoplasma hyopneumoniae (enzootic pneumonia) onto pig farms in areas in switzerland that were involved in an eradication programme from 1996 to 1999 and to assess the role of dealers in relation to these reinfections. the study was based on the comparison of pig farms that were reinfected in the year 2000 (cases) and pig farms that remained uninfected in the same area (controls). add ... | 2002 | 12564544 |
| molecular characterization of the pig c3 gene and its association with complement activity. | the complement system catalyzes direct lysis of micro-organisms and modulates phagocytosis, inflammation, humoral and cellular immune responses. since the complement protein c3 is the central component within all pathways of complement activation, c3 is a candidate gene for complement activity and also for improved protection against many pathogens. the pig c3 gene was sequenced, screened for polymorphisms, and analyzed for association with hemolytic complement activity of the alternative and cl ... | 2003 | 12557058 |
| vaccine efficacy of the attenuated erysipelothrix rhusiopathiae ys-19 expressing a recombinant protein of mycoplasma hyopneumoniae p97 adhesin against mycoplasmal pneumonia of swine. | the attenuated erysipelothrix rhusiopathiae ys-19 strain was constructed for the purpose of delivering the c-terminal portion of the mycoplasma hyopneumoniae p97 adhesin to the mucosal surface of the respiratory tract of pigs. in this study, the efficacy of the ys-19 vaccine against mycoplasmal pneumonia of swine was evaluated. animal experiments revealed that intranasal immunization of pigs with the ys-19 strain significantly reduced the severity of pneumonic lung lesions caused by m. hyopneumo ... | 2003 | 12531653 |
| studies of the field efficacy and safety of a single-dose mycoplasma hyopneumoniae vaccine for pigs. | the field efficacy and safety of a single-dose mycoplasma hyopneumoniae vaccine were evaluated in three-to five-week-old pigs. two field efficacy studies were conducted, one in england with 673 pigs, and one in germany with 719 pigs. the pigs were injected intramuscularly with either the vaccine or saline (control) at a ratio of 2:1 and reared under commercial conditions to slaughter weight. the efficacy of the vaccine was evaluated by comparing the lung lesions associated with infection with m. ... | 2002 | 12448490 |
| porcine circovirus type 2 (pcv-2) coinfections in us field cases of postweaning multisystemic wasting syndrome (pmws). | the prevalence of different pathogens detected in combination with porcine circovirus type 2 (pcv-2) was studied retrospectively in field cases of postweaning multisystemic wasting syndrome (pmws) diagnosed at the iowa state university veterinary diagnostic laboratory, ames, iowa, between january 2000, and september 2001. the presence of pcv-2 antigen in lymphoid tissues and/or lung, demonstrated by immunohistochemistry, together with moderate to severe lymphoid depletion and/or granulomatous ly ... | 2002 | 12423038 |
| use of a mycoplasma hyopneumoniae nested polymerase chain reaction test to determine the optimal sampling sites in swine. | a number of polymerase chain reaction (pcr)-based diagnostic tests have been developed for mycoplasma hyopneumoniae, including one from this research group. this report presents further development, optimization, and standardization of a nested pcr test. detection sensitivity was 1 fg of m. hyopneumoniae chromosomal dna (approximately 1 organism). this exceeded the sensitivity of or compared favorably with other published pcr tests. polymerase chain reaction primers to porcine beta2-microglobuli ... | 2002 | 12423027 |
| patterns of mycoplasma hyopneumoniae infections in belgian farrow-to-finish pig herds with diverging disease-course. | patterns of mycoplasma hyopneumoniae (mh) infections were investigated in five clinically infected herds and in five herds subclinically infected with mh. in the clinically infected herds, housing and management conditions were good whereas these conditions were poor in the subclinically infected herds. in each herd, serum antibodies against mh were detected in pigs of different ages and nasal swabs were taken for mh detection using nested pcr (npcr). the percentage of seropositive pigs in the c ... | 2002 | 12420871 |
| analysis of heat shock protein 60 encoding genes of mycoplasmas and investigations concerning their role in immunity and infection. | only little is known about the heat shock proteins (hsp) and hsp-encoding genes of mycoplasmas. the aim of this study was to identify and sequence the hsp60 gene of mycoplasma agalactiae, mycoplasma arthritidis, mycoplasma bovis, and mycoplasma hyopneumoniae, and to investigate the immune response to hsp60. fragments of the hsp60 genes of m. agalactiae, m. arthritidis, m. bovis and m. hyopneumoniae representing almost the entire coding region were amplified by pcr. two fragments of a hsp60 gene ... | 2002 | 12243891 |
| chronologic localization of mycoplasma hyopneumoniae in experimentally infected pigs. | the chronologic localization of mycoplasma hyopneumoniae was examined by in situ hybridization in experimentally infected pigs for a period of 35 days after intratracheal inoculation. m. hyopneumoniae dna was detected in bronchial and bronchiolar epithelial cells from infected pigs at 7, 14, 21, and 28 days postinoculation (dpi) and in alveolar and interstitial macrophages and type i pneumocytes from infected pigs at 14, 21, 28, and 35 dpi. strong hybridization signals for m. hyopneumoniae were ... | 2002 | 12243470 |
| antibodies to selected viral and bacterial pathogens in european wild boars from southcentral spain. | serum samples from 78 european wild boars (sus scrofa) harvested during the 1999-2000 hunting season were tested for antibodies to brucella spp., classical swine fever virus, erysipelothrix rhusiopathiae, haemophilus parasuis, leptospira interrogans serovar pomona, mycoplasma hyopneumoniae, pseudorabies virus (prv), porcine parvovirus (ppv), porcine reproductive and respiratory syndrome virus, salmonella serogroups b, c, and d, streptococcus suis, and swine influenza virus (siv) serotypes h1n1 a ... | 2002 | 12238391 |
| effectiveness of treatment with lincomycin hydrochloride and/or vaccination against mycoplasma hyopneumoniae for controlling chronic respiratory disease in a herd of pigs. | a herd of pigs infected with mycoplasma hyopneumoniae was used in a double-blind randomised trial to assess the effectiveness of three control strategies against chronic respiratory disease in growing-finishing pigs. one group of 61 pigs received 220 ppm lincomycin hydrochloride in the feed from day 71 to day 91, a second group was vaccinated against m. hyopneumoniae at four and 28 days of age, and a third group received both treatments; a fourth group was left untreated as a control. throughout ... | 2002 | 12199431 |
| mycobacterial esat-6 protein enhances mouse ifn-gamma responses to mycoplasma hyopneumoniae p71 protein. | type 1 immune responses play an important role in the resolution of diseases with infectious or oncogenic etiologies. vaccines for production animals frequently target humoral immune responses and are often ineffective in protecting against disease. in order to shift the immune response more toward cellular immunity (i.e., type 1 response), we tested the ability of a mycobacterial protein, early secretory antigenic target (esat-6), to enhance interferon-gamma (ifn-gamma) secretion during the rec ... | 2002 | 12184919 |
| in vivo and in vitro comparisons of spray-drying and solvent- evaporation preparation of microencapsulated mycoplasma hyopneumoniae for use as an orally administered vaccine for pigs. | to evaluate the efficacy of an orally administered vaccine of mycoplasma hyopneumoniae that was prepared by spray drying or solvent evaporation. | 2002 | 12171164 |
| infectious and rearing-system related risk factors for chronic pleuritis in slaughter pigs. | chronic pleuritis (cp) in danish pigs for slaughter is by far the most frequent finding at the routine post-mortem meat inspection. an initial investigation published in 1990 demonstrated infectious and management-related risk factors. serological testing for additional infectious agents, as well as the need to consider the effect of disease clustering at the herd level, required a re-analysis of the data. our re-analysis used a representative sample of 4,800 pigs originating from 623 danish her ... | 2002 | 12163250 |
| a meta-analysis comparing the effect of vaccines against mycoplasma hyopneumoniae on daily weight gain in pigs. | our aims were to evaluate the published literature concerning the effect of swine vaccination against mycoplasma hyopneumoniae on the average daily weight gain (adwg). this was done by re-evaluating the influence of selected factors on adwg by a meta-analysis of published studies from 1991 to 1999, fulfilling certain inclusion criteria. with adwg as the outcome, an analysis of variance was performed for such variables as treatment, vaccination schedule, age during study, housing system and publi ... | 2002 | 12114013 |
| in vivo studies on cytokine involvement during acute viral respiratory disease of swine: troublesome but rewarding. | the early cytokines interferon-alpha (ifn-alpha), tumour necrosis factor-alpha (tnf-alpha), interleukin-1, -6 and -8 (il-1, -6, -8) are produced during the most early stage of an infection. the activities of these cytokines have been studied extensively in vitro and in rodents, but in vivo studies on the role of these cytokines in infectious diseases of food animals are few. this review concentrates on in vivo studies of cytokine involvement in infectious respiratory diseases of swine, with an e ... | 2002 | 12072230 |
| mycoplasma hyopneumoniae colonization of pigs sired by different boars. | differences in mycoplasma hyopneumoniae colonization were evaluated in experimentally inoculated pigs sired by 3 different boars of the same genetic line. forty-six pigs were used, including a treatment group and positive and negative control groups. the pigs were intratracheally inoculated with an m. hyopneumoniae suspension or with friis media as a placebo. to evaluate differences in the magnitude of colonization during a 35-day period, nasal and tracheal swabs were collected weekly and tested ... | 2002 | 11989738 |
| mycoplasma hyopneumoniae increases intracellular calcium release in porcine ciliated tracheal cells. | we investigated the effects of intact pathogenic mycoplasma hyopneumoniae, nonpathogenic m. hyopneumoniae, and mycoplasma flocculare on intracellular free ca2+ concentrations ([ca2+]i) in porcine ciliated tracheal epithelial cells. the ciliated epithelial cells had basal [ca2+]i of 103 +/- 3 nm (n = 217 cells). the [ca2+]i increased by 250 +/- 19 nm (n = 47 cells) from the basal level within 100 s of the addition of pathogenic m. hyopneumoniae strain 91-3 (300 microg/ml), and this increase laste ... | 2002 | 11953388 |
| in situ hybridisation for identification and differentiation of mycoplasma hyopneumoniae, mycoplasma hyosynoviae and mycoplasma hyorhinis in formalin-fixed porcine tissue sections. | oligonucleotide probes targeting 16s ribosomal rna were designed for species-specific identification of the porcine mycoplasmas mycoplasma hyopneumoniae, mycoplasma hyorhinis and mycoplasma hyosynoviae using a fluorescent in situ hybridisation assay. the specificity of the probes was evaluated using pure cultures as well as porcine tissue sections with artificial presence of mycoplasma, and the probes were found specific for the target organisms. the assay was applied on sections of 28 tissue sa ... | 2001 | 11890568 |
| regional eradication of mycoplasma hyopneumoniae from pig herds and documentation of freedom of the disease. | the objectives of this study were to 1) screen all sow herds in a region for m. hyopneumoniae, 2) to effectuate an eradication programme in all those herds which were shown to be infected with m. hyopneumoniae, and 3) to follow the success of the screening and the eradication programmes. the ultimate goal was to eradicate m. hyopneumoniae from all member herds of a cooperative slaughterhouse (153 farrowing herds + 85 farrowing-to-finishing herds + 150 specialised finishing herds) before year 200 ... | 2001 | 11887396 |
| growth performance and whole-body composition of pigs experimentally infected with mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae (mh) is the primary infectious pathogen responsible for enzootic pneumonia in pigs. although mh is thought to impair growth performance, whole-body composition, and fat and protein accretion in pigs with pneumonia have not been reported and the mechanism through which mh reduces growth is unknown. the objectives of this study were to evaluate the effects of mh on growth performance, whole-body composition, and protein and fat accretion in nursery pigs and to determine wh ... | 2002 | 11883429 |
| a comparative study of the preventive use of tilmicosin phosphate (pulmotil premix) and mycoplasma hyopneumoniae vaccination in a pig herd with chronic respiratory disease. | this study was conducted to compare the effects of a preventive in-feed medication programme using tilmicosin (pulmotil 200 premix, elanco animal health) at 200 p.p.m. with those of mycoplasma hyopneumoniae (mh) vaccination programme (stellamune mycoplasma, pfizer animal health). a pig herd with chronic respiratory disease in which infection with mh played an important role was selected, and a total of 204 piglets were randomly allocated to either the medication (p) or the vaccination (v) group. ... | 2001 | 11846018 |
| emergency-culling and mortality in growing/fattening pigs in a large hungarian "farrow-to-finish" production unit. | the trial was carried out in a large hungarian 2000 sow "farrow-to-finish" production unit. out of a total number of 15,841 evaluated growing/finishing pigs, 1319 pigs died or were emergency-culled and were post-mortem routinely examined. for the purpose of this study the emergency-culling and mortality were recorded into the same category. the average annual emergency-culling/mortality rate was unusual high (8.62%). five body condition related categories were registered. the majority of the mor ... | 2002 | 11838293 |
| induction of enzootic pneumonia in pigs by the administration of an aerosol of in vitro-cultured mycoplasma hyopneumoniae. | the aim of this study was to establish whether enzootic pneumonia could be induced reliably in piglets by administering an aerosolised culture of mycoplasma hyopneumoniae. groups of five m hyopneumoniaefree landrace x large white piglets weaned at 11 to 14 days of age were exposed to aerosols of in vitro cultures of a virulent strain of m hyopneumoniae. in three separate trials, 14 of 15 pigs exposed to the bacteria developed pneumonia, but pigs exposed to the culture medium alone did not develo ... | 2002 | 11817868 |
| the effects of immuno-modulation on the clinical and pathological expression of postweaning multisystemic wasting syndrome. | post-weaning multisystemic wasting syndrome (pmws), primarily caused by porcine circovirus type 2 (pcv-2), is an economically important disease of pigs in many countries. a trial was designed to investigate the hypothesis that non-specific immuno-modulation can influence the clinical and pathological expression of pmws in pigs naturally infected with pcv-2. eighty-four pigs on a commercial pig farm were allocated to three groups of 28 pigs each, during an outbreak of pmws. the pigs in the first ... | 2002 | 11814320 |
| treatment of pigs experimentally infected with mycoplasma hyopneumoniae, pasteurella multocida, and actinobacillus pleuropneumoniae with various antibiotics. | the authors have performed a comparative study of the efficacy of various in-feed medications for the treatment of 5- to 6-week-old specific pathogen-free (spf) piglets experimentally infected on day 1 with mycoplasma hyopneumoniae, on day 8 with pasteurella multocida (serotype a), and on day 15 with actinobacillus pleuropneumoniae (serotype 2). the treatment started on day 9 and continued for 12 consecutive days, then the piglets were euthanized for examination of macroscopic, histologic, and p ... | 2001 | 11768127 |
| field study on the efficacy of two different vaccination schedules with hyoresp in a mycoplasma hyopneumoniae-infected commercial pig unit. | a trial was carried out with hyoresp a mycoplasma hyopneumoniae (m. hyo) vaccine in order to confirm the benefit of vaccination under field conditions in a commercial industrial farrow-to-finish unit, contaminated with m. hyo. infection with m. hyo was confirmed through positive blood and colostrum samples [enzyme-linked immunosorbent assay (elisa) test] combined with positive gross lesions of the lung at slaughter. two different vaccination schedules were tested. pigs were randomly allocated to ... | 2001 | 11765803 |
| erysipelothrix rhusiopathiae ys-1 as a live vaccine vehicle for heterologous protein expression and intranasal immunization of pigs. | we have developed a system in which a foreign antigen is delivered and expressed on the surface of an attenuated strain of erysipelothrix rhusiopathiae ys-1 and have examined the ability of a such recombinant e. rhusiopathiae strain to function as a mucosal vaccine vector. the c-terminal portion, including two repeat regions, r1 and r2, of the p97 adhesin of mycoplasma hyopneumoniae strain e-1 was successfully translocated and expressed on the e. rhusiopathiae ys-1 cell surface after it was fuse ... | 2002 | 11748187 |
| evaluation of conjugated linoleic acid and dietary antibiotics as growth promotants in weanling pigs. | an experiment was conducted to determine the efficacy of dietary conjugated linoleic acid (cla) as a growth promotant in weanling swine. weanling pigs (n = 192; 7.6 kg and 29 d of age) were randomly assigned to four treatments that were arranged as a 2 x 2 factorial. concentrations of dietary cla (0 or 0.6%) and antibiotics (+/-) constituted the main effect variables. dietary cla treatments consisted of a 1% addition of an oil containing 60% cla isomers or 1% soybean oil, and dietary antibiotic ... | 2001 | 11721832 |