Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| temperature-induced alterations in 8-anilino-1-naphthalenesulfonate fluorescences with membranes from mycobacterium phlei. | 1974 | 4586935 | |
| mode of action of rifampin on mycobacteria. ii. biosynthetic studies on the inhibition of ribonucleic acid polymerase of mycobacterium bovis bcg by rifampin and uptake of rifampin- 14 c by mycobacterium phlei. | 1973 | 4576356 | |
| susceptibility of rabbits immunized with mycobacterium bovis (bcg) or mycobacterium phlei to shigella keratoconjunctivitis. | vaccination of rabbits with mycobacteria increased their resistance to eye infection with shigella flexneri. however, the severity of keratoconjunctivitis was not reduced in the vaccinated animals. | 1972 | 4565052 |
| synchronized growth of mycobacterium phlei. | 1972 | 4555268 | |
| transduction in mycobacterium phlei. | 1973 | 4540257 | |
| complex formation between mycobacterial polysaccharides and fatty acyl-coa derivatives. | the mycobacterial polysaccharides mmp and mglp, which contain numerous o-methyl-sugar residues, markedly stimulate fatty acid synthesis catalyzed by a multienzyme complex from mycobacterium phlei [ilton, m. et al. (1971) proc. nat. acad. sci. usa 68, 87-91]. when aqueous solutions containing mmp or mglp and palmitoyl-coa were chromatographed on sephadex g-75 under conditions that widely separate the individual components, polysaccharide and fatty acyl-coa were eluted in a single peak, indicating ... | 1973 | 4515612 |
| studies on inorganic polyphosphate metabolism in mycobacterium phlei. ii. direct utilization of inorganic polyphosphates for synthesis of nucleic acids in m. phlei. | 1974 | 4462736 | |
| separability of enzymes of the common aromatic biosynthetic pathway in mycobacterium phlei. | 1974 | 4447810 | |
| 3-deoxy-d-arabino-heptulosonate-7-phosphate synthetase of mycobacterium phlei: partial purification and feedback inhibition. | 1974 | 4447807 | |
| the formation of 6-ethylsalicylic acid by mycobacterium phlei. | 1974 | 4433717 | |
| the translocation of energy-rich phosphate in mycobacterium phlei protoplast ghosts by a soluble protein fraction. | 1974 | 4430684 | |
| mutagenesis by n-methyl-n-nitroso-n'-nitroguanidine in synchronized cultures of mycobacterium phlei. | 1974 | 4430476 | |
| active transport of proline in membrane preparations from mycobacterium phlei. | 1974 | 4425314 | |
| purification and properties of a membrane-bound phospholipase a1 from mycobacterium phlei. | 1974 | 4415399 | |
| appearance of an inhibitor of rna polymerase activity in thermophilic mycobacterium phlei exposed to sublethal temperatures. | 1974 | 4412068 | |
| [intermediary metabolism of mycobacteria. i. comparative study of 2 "metabolic snaps" realized respectively on mycobacterium phlei and mycobacterium tuberculosis, strain h37ra]. | 1970 | 4392117 | |
| properties of a new tpn-like electron transport component from mycobacterium phlei. | 1964 | 4378502 | |
| the effect of sulfhydryl inhibitors on substrate oxidation and proline transport with membrane preparations from mycobacterium phlei. | 1974 | 4370899 | |
| relationship of a proton gradient to the active transport of proline with membrane vesicles from mycobacterium phlei. | electron transport particles prepared from mycobacterium phlei were depleted of bound coupling factors by washing with water in the absence of inorganic ions. the depleted electron transport particles were void of latent atpase activity and were capable of oxidation, but were unable to support coupled phosphorylation. nevertheless, the depleted electron transport particles were capable of substrate-induced active transport of proline. changes in ph in response to substrate oxidation were measure ... | 1974 | 4364528 |
| multiple cytochromes b in mycobacterium phlei. | 1973 | 4356985 | |
| quantitative extraction of adenosine triphosphate from cultivable and host-grown microbes: calculation of adenosine triphosphate pools. | existing data on adenosine triphosphate (atp) pools in microbes are deficient for two reasons: (i) incomplete extractions of atp, and (ii) the failure to take into account that the adverse effects of extracting procedures on standard atp exert analogous effects on the atp released from bacterial cells. methods for correcting observed yields and calculating atp pools have been demonstrated. three bacterial species were used in the studies on extraction of atp: escherichia coli, mycobacterium phle ... | 1973 | 4356463 |
| ph profiles of proline transport with membrane vesicles from mycobacterium phlei with artificial and natural electron donors. | 1973 | 4350062 | |
| orientation of the cell membrane in ghosts and electron transport particles of mycobacterium phlei. | 1973 | 4349862 | |
| the 6-o-methylglucose-containing lipopolysaccharides of mycobacterium phlei. locations of the acyl groups. | 1972 | 4344992 | |
| the effects of technical chlordane on growth and energy metabolism of streptococcus faecalis and mycobacterium phlei: a comparison with bacillus subtilis. | 1971 | 4337270 | |
| a factor(s) required for activation of oxidative phosphorylation in protoplast ghosts of mycobacterium phlei. | 1972 | 4334978 | |
| an extracellular product of mycobacterium phlei. 3. relationship of production to microbial growth and evidence for a similar substance in other mycobacteria. | 1972 | 4334844 | |
| respiration dependent transport of proline by electron transport particles from mycobacterium phlei. | 1971 | 4334137 | |
| oxidative phosphorylation in fractionated bacterial systems: effect of chloramphenicol. | chloramphenicol was found to have a direct effect on the respiratory chain of mycobacterium phlei cells grown in the presence of this drug. analysis of the respiratory chain components revealed that the presence of chloramphenicol during growth resulted in a partial inhibition in the synthesis of the cytochromes. however, a stimulation in oxidative phosphorylation was observed with the cell-free extract of cells grown in the presence of chloramphenicol. the oxidation of succinate was found to be ... | 1971 | 4333316 |
| resolution and reconstitution of the succinoxidase pathway of mycobacterium phlei. | 1971 | 4332729 | |
| effect of n,n'-dicyclohexylcarbodiimide (dccd) on electron transport particles of mycobacterium phlei. | 1971 | 4332727 | |
| reversal of the effects of freezing on oxidative phosphorylation in the mycobacterium phlei system. | 1971 | 4327443 | |
| an extracellular product of mycobacterium phlei. ii. its effect on the mitochondrial nadh dehydrogenase and tetrazolium reductase systems. | 1971 | 4323770 | |
| anaerobic transformation of steroids by mycobacterium phlei. | 1970 | 4323640 | |
| the effect of trypsin and heat treatment on oxidative phosphorylation in mycobacterium phlei. | 1970 | 4320074 | |
| oxidative phosphorylation in fractionated bacterial systems. xxix. the involvement of nonheme iron in the respiratory pathways of mycobacterium phlei. | 1967 | 4319674 | |
| cytochromes from mycobacterium phlei. i. isolation and spectral properties of a mixture of cytochromes (a + a3) (o). | 1970 | 4319456 | |
| effects of heat treatment of electron-transport particles on bacterial oxidative phosphorylation. | the electron-transport particles from mycobacterium phlei exhibit low levels of phosphorylation unless supplemented with soluble coupling proteins. heat treatment of the electron transport particles for 15 min at 50 degrees c was found to result in a slight loss of oxidation and an activation of phosphorylation with nadh as substrate, while with succinate as substrate both activities increased. the heat-activated particles do not require the addition of soluble coupling factors and their level o ... | 1970 | 4318778 |
| enzymatic alkylenation of phospholipid fatty acid chains by extracts of mycobacterium phlei. | 1970 | 4313604 | |
| [presence of polyunsaturated acids in bacteria: isolation of hexatriacontapentaene-4,8,12,16,20-oic acid and acid analogs from mycobacterium phlei lipids]. | 1969 | 4310547 | |
| phosphorylation linked to ascorbate oxidation in mycobacterium phlei. | 1969 | 4310220 | |
| new light-sensitive cofactor required for oxidation of succinate by mycobacterium phlei. | irradiation of the electron transport particles of mycobacterium phlei with light at a wavelength of 360 manometers resulted in a loss of oxidase activities of succinate and the reduced form of nicotinamide adenine dinucleotide. the lesion in the two pathways caused by irradiation of the particles differs. the succinoxidase pathway was more labile to irradiation than the pathway linked to nicotinamide adenine dinucleotide. restoration of succinoxidase activity (up to 50 to 60 percent) occurred o ... | 1969 | 4304858 |
| separation of a factor indispensable for coupled phosphorylation from the particulate fraction of mycobacterium phlei. | 1969 | 4304303 | |
| exogenous nadh oxidation and particulate fumarate reductase in mycobacterium phlei. | 1969 | 4303095 | |
| induction of mannitol dissimilation in mycobacterium phlei. | 1968 | 4297733 | |
| biosynthesis of mannophosphoinositides by mycobacterium phlei. enzymatic acylation of the dimannophosphoinositides. | 1968 | 4297467 | |
| the 6-o-methylglucose-containing lipopolysaccharide of mycobacterium phlei. identification of d-glyceric acid and 3-o-methyl-d-glucose in the polysaccharide. | 1968 | 4295929 | |
| cis-trans isomerism in naphthoquinones: interconversion and participation in oxidative phosphorylation. | synthetic phylloquinone was resolved into cis and trans isomers by thin-layer chromatography. the two isomers had identical ultraviolet spectra characteristic of vitamin k(1) and were differentiated by nuclear-magnetic-resonance spectroscopy on the basis of the displacement of the peak corresponding to the olefinic methyl group in the naphthoquinone side chain. studies on the restoration of electron transport coupled to phosphorylation in irradiated preparations of mycobacterium phlei showed tha ... | 1967 | 4293760 |
| bacterial carotenoids xx. the carotenoids of mycobacterium phlei strain vera. 2. the structures of the phlei-xanthophylls--two novel tertiary glucosides. | 1967 | 4291656 | |
| biosynthesis of mannophosphoinositides by mycobacterium phlei. the family of dimannophosphoinositides. | 1967 | 4291069 | |
| oxidation of malate by mycobacterium phlei extracts. | 1965 | 4287523 | |
| biosynthesis of mannophospholipids by mycobacterium phlei. | 1966 | 4285847 | |
| search for inducible sugar kinases in mycobacterium phlei. | 1969 | 4253716 | |
| [carbohydrate metabolism in mycobacterium phlei and mycobacterium tuberculosis]. | 1973 | 4198569 | |
| [common antigenic fractions revealed by immunoprecipitation in gel, between mycobacterium phlei (mycobacteriaceae) and leishmania donovani (trypanosomidae)]. | 1972 | 4197659 | |
| oxidation of reduced nicotinamide adenine dinucleotide by mycobacterium phlei. | 1969 | 4189893 | |
| production of antibodies against mycobacterium phlei phage. | 1969 | 4187018 | |
| isolation and some properties of reduced diphosphopyridine nucleotide: 2,6-dichlorophenolindophenol soluble reductase from mycobacterium phlei. | 1967 | 4168405 | |
| enzymic activation of d-glutamic acid and d-alanine in mycobacterium phlei. | 1966 | 4160453 | |
| oxidative phosphorylation in fractionated bacterial systems. 18. phosphorylation coupled to different segments of the respiratory chains of mycobacterium phlei. | 1965 | 4158502 | |
| electron transfer mechanism involved in stearoyl-coenzyme a desaturation by particulate fraction of mycobacterium phlei. | 1973 | 4148376 | |
| acetyl-coa and propionyl-coa carboxylation by mycobacterium phlei. partial purification and some properties of the enzyme. | 1973 | 4147495 | |
| electronmicroscopic comparison of the acid-fast and non-acid-fast strain of mycobacterium phlei. | 1974 | 4136216 | |
| studies on the effect of starvation on mycobacteria. | ten cultures of mycobacterium tuberculosis, one of mycobacterium kansasii (nonsignificant), and one of mycobacterium phlei were submitted to starvation. as a result they lost first their acid fastness and then all other staining affinities but, in this chromophobic state, they survived for at least 2 years and, after that time, produced cultures of acid-fast bacilli when transferred onto nutrient media. chromophobic tubercle bacilli similar to those produced experimentally had previously been de ... | 1974 | 4132910 |
| [antigenic study of the "amorphous" layer, the outermost layer of mycobacterium phlei]. | 1971 | 4122828 | |
| [purification of l asparaginase from mycobacterium phlei and demonstration of antigenic similarity to the escherichia coli enzyme]. | 1972 | 4119672 | |
| [use of mycobacterium phlei of differentiated resistance for the detection of ethambutol and capreomycin in the sera of tuberculous patients]. | 1972 | 4113117 | |
| an extracellular product of mycobacterium phlei. i. production, recovery, and characterization. | 1971 | 4101357 | |
| the effect of daylight on the biosynthesis of carotenoids by non-acid-fast strains of mycobacterium phlei. | 1970 | 4097568 | |
| [antibacterial properties of some spice plants before and after heat treatment]. | this study was carried out to understand the antibacterial properties of some spice plants before and after heat treatment in boiling water. the samples included the core and the outer layers of onion, the white and the green parts of green onion, garlic bulb, ginger, ginger root, sweet pepper, chili pepper, brown pepper, and mustard. the test microorganisms included escherichia coli, salmonella typhimurium, vibrio parahaemolyticus, pseudomonas aeruginosa, proteus vulgaris, staphylococcus aureus ... | 1985 | 4064797 |
| nonspecific immunostimulation against viruses. | a trypsinized preparation from chromogenic selected strain of mycobacterium phlei (nsi) stimulated the recipient immune system non-specifically against a variety of viruses viz. rabies virus (rna virus). marek's disease (dna virus) and foot and mouth disease virus (rna virus) in phylogenetically different hosts like mice, chicks and guinea pigs respectively. investigation into mechanisms of such nonspecific immunostimulation revealed that there was induction of strong cell mediated immune respon ... | 1985 | 4064625 |
| absence of mycobactin in mycobacterium leprae; probably a microbe dependent microorganism implications. | ferric mycobactins were prepared from mycobacterium phlei. mycobacterium avium--intracellulare a and h, isolated respectively from armadillo and human leprosy specimens. attempts were made to extract mycobactin from host grown m. leprae cells. the crude ferric mycobactin extracts were tested for growth supporting effect on the mycobactin dependent m. paratuberculosis strain atcc 19698. mycobactins prepared from m. phlei and the two m. avium--intracellulare strains had growth promoting effect on ... | 1985 | 3897405 |
| a simple technique for increased sensitivity in immunoelectrophoresis by the use of antiserum against igg. | precipitates in two-dimensional immunoelectrophoresis (2d-ie) plates can be amplified by the application of an antiserum against igg after the second dimension run. thereby, the sensitivity of the ie method is increased; faint precipitates and antigen-antibody reactions close to but below the level of forming recognizable precipitates are made visible. the advantage of the method was demonstrated for a multilinear mycobacterium phlei system. | 1987 | 3819446 |
| purification and characterization of dihydrofolate reductase from mycobacterium phlei. | the dihydrofolate reductase from mycobacterium phlei was purified and characterized; it has an mr of 15 000 and a pi of 4.8. it is competitively inhibited by both methotrexate and trimethoprim, although the affinity is less than for other bacterial dihydrofolate reductases. | 1986 | 3741386 |
| solubilization, purification, and characterization of succinate dehydrogenase from membranes of mycobacterium phlei. | succinate dehydrogenase (sdh) was solubilized from membranes of mycobacterium phlei by triton x-100 with a recovery of about 90%. the solubilized sdh was purified about 90-fold by sephacryl s-300, deae-cellulose, hydroxylapatite, and isoelectric focusing in the presence of triton x-100 with a 20% recovery. sdh was homogeneous, as determined by polyacrylamide gel electrophoresis in nondenaturing gels containing triton x-100. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the enzyme ... | 1986 | 3722123 |
| isolation and characterization of the facultative methylotroph mycobacterium id-y. | a facultatively methylotrophic mycobacterium was isolated from cleveland harbor, ohio, usa. the isolate, designated id-y, used a wide range of carbon and energy sources including methane and several other hydrocarbons. it displayed a growth cycle from rod-shaped exponential-phase cells, with many cell pairs exhibiting v-formation, to cocco-bacillary stationary-phase cells. a fixation technique involving glutaraldehyde/alcian blue resulted in the observation of a three-layered cell wall. isolate ... | 1987 | 3655742 |
| patterns and distribution of aminoglycoside-acetylating enzymes in rapidly growing mycobacteria. | cell-free extracts from clinical, laboratory, and environmental isolates of mycobacterium fortuitum, mycobacterium smegmatis, mycobacterium phlei, and mycobacterium vaccae were tested for the presence of aminoglycoside-acetylating enzyme and compared with enzymes from gram-negative organisms. acetylating activity was detected in all strains examined despite variable levels of aminoglycoside susceptibility. substrate profiles revealed 2 different patterns of 3-n-acetyltransferase. one pattern exh ... | 1987 | 3619192 |
| antimicrobial activity of benzylisoquinoline alkaloids. | the antimicrobial in vitro activity of 14 benzylisoquinoline alkaloids was investigated by agar diffusion and agar dilution methods against several genera of microorganisms that included streptococcus, staphylococcus, bacillus, lysteria, escherichia, salmonella, klebsiella, pseudomonas, enterobacter, serratia, shigella, mycobacterium and candida. anolobine was the most active compound against grampositive bacteria with mic90 between 12 and 50 mg/l; less active were anonaine, lysicamine and lirio ... | 1987 | 3615557 |
| nadp-specific isocitrate dehydrogenase of mycobacterium phlei atcc 354: purification and characterization. | nadp-dependent isocitrate dehydrogenase (ec 1.1.1.42) from mycobacterium phlei atcc 354 was purified to homogeneity by ammonium sulphate fractionation, followed by deae cellulose and sephadex g-200 chromatography. the ph optimum of the enzyme was 8.5. the km values for isocitrate and nadp were 74 and 53 microm, respectively. mn2+ was essential for enzyme activity. the enzyme lost all activity on incubation at 70 degrees c for 15 min; isocitrate and nadp protected against this thermal inactivatio ... | 1987 | 3448153 |
| analysis by elisa and western blotting of antibody reactivities in cattle infected with mycobacterium paratuberculosis after absorption of serum with m phlei. | preabsorption of cattle serum with mycobacterium phlei was of value in eliminating falsely positive reactions in an enzyme-linked immunosorbent assay (elisa) for the detection of antibodies against m paratuberculosis. specific antibody titres from 16 animals naturally infected with m paratuberculosis were unaffected by absorption. analysis by western blotting indicated that a different set of antigens of m paratuberculosis were recognised by serum from falsely positive reactors compared with tha ... | 1987 | 3296062 |
| analysis of fatty acids by negative ion gas chromatography/tandem mass spectrometry: structural correlations between alpha-mycolic acid chains and delta-5-monounsaturated fatty acids from mycobacterium phlei. | the analysis of a complex mixture of monounsaturated fatty acids from mycobacterium phlei is achieved by capillary gas chromatographic separation of their pentafluorobenzyl esters, formation of gas-phase carboxylate anions by electron capture ionization and decomposition of these anions by collision activation. this method allows the structural determination of fatty acid isomers by examination of their collisionally activated dissociation mass-analysed ion kinetic energy spectra. a good correla ... | 1988 | 3242686 |
| isolation and structural characterization of a new non-phosphorylated lipoamino acid from mycobacterium phlei. | a new non-phosphorylated lipoamino acid was extracted from mycobacterium phlei, strain ist. it is particularly sensitive to alkaline hydrolysis, and contains a lysine residue joined to a 1,2-diglyceride via an ester linkage. the fab-positive mass spectrum shows the presence of various molecular species of which the most abundant contains a palmitic and a tuberculostearic acid residue. an analogue of this lipid was synthesized, 1,2-dipalmitoyl 3-lysyl glycerol. both its chromatographic behavior ( ... | 1988 | 3240562 |
| influence of oxygen tension on the respiratory activity of mycobacterium phlei. | growth of mycobacterium phlei under low oxygen tension resulted in specific activities two to twenty times lower for formate dehydrogenase, malate dehydrogenase, beta-hydroxybutyrate dehydrogenase, lactate oxidase and nadh dehydrogenase than when cultures were grown under high aeration. an increase in fumarate reductase and succinate dehydrogenase occurred with m. phlei grown under low oxygen tension. malate: vitamin k dehydrogenase and glucose-6-phosphate dehydrogenase activity were not signifi ... | 1988 | 3183614 |
| differentiation of mycobacterium thermoresistibile from mycobacterium phlei and other rapidly growing mycobacteria. | mycobacterium thermoresistibile is the only chromogenic rapidly growing mycobacterial species reported to cause infections in humans and animals. dna-dna hybridization (s1 nuclease method) showed that m. thermoresistibile formed a dna relatedness group which was only 24 to 30% related to m. phlei. alkaline phosphatase and beta-galactosidase differentiated m. thermoresistibile from m. phlei. among the rapidly growing mycobacteria, the mycolic acid pattern of m. thermoresistibile was unique (alpha ... | 1986 | 3122636 |
| phenotypic changes in mycobacteria grown in oxygen-limited conditions. | laboratory strains of mycobacterium phlei, m. smegmatis, m. fortuitum, m. gordonae, m. kansasi, m. bovis, m. tuberculosis and m. intracellulare were adapted to grow in an anaerobic environment. concomitant with the transition to anaerobic growth was loss of acid-fastness, loss or modification of colonial pigmentation, and loss of ability to grow on a malachite green-containing medium. the mycobacteria grown anaerobically produced acid from a greater range of carbohydrates than aerobically grown ... | 1986 | 3084791 |
| evidence for genetic divergence in ribosomal rna genes in mycobacteria. | dna was isolated from mycobacterium phlei and from m. smegmatis. each dna sample was restricted with endonucleases, the fragments were separated by agarose gel electrophoresis and transferred to nitrocellulose film. fragments of dna containing rrna sequences were identified by means of 125i-labelled rrna of m. phlei or of m. smegmatis. the distributions of restriction endonuclease sites within the rrna gene(s) and flanking sequences were found to be characteristic for each of the two species. hy ... | 1986 | 3002853 |
| 4-(2'-carboxyphenyl)-4-oxobutyryl coenzyme a ester, an intermediate in vitamin k2 (menaquinone) biosynthesis. | enzyme preparations from mycobacterium phlei, escherichia coli and galium mollugo cell suspension cultures were incubated in the presence of 4-(2'-carboxyphenyl)-4-oxobutyrate (i.e. o-succinylbenzoic acid, osb, 1), atp, coenzyme a and mg2+. the main product isolated from the incubation mixture was 4-(2'-carboxyphenyl)-4-oxobutyryl coenzyme a ester (2) as determined by comparison with synthetic coenzyme a esters. synthetic and enzymically formed 4-(2'-carboxyphenyl)-4-oxobutyryl coenzyme a ester ... | 1987 | 2966501 |
| aspects of mycobacterial response to beryllate ions in vitro. | comparative growth measurements of the mycobacterial species mycobacterium phlei and mycobacterium tuberculosis were carried out with liquid cultures supplemented with oxalatoberyllate ions in the concentration range 0-120 microm be. for both species, the effect of beryllium on specific growth parameters can be represented by a be concentration-dependent, exponential expression. evidence for beryllium binding to cellular phospholipids (approximately 15% of be uptake) is provided by 31p nmr spect ... | 1986 | 2946115 |
| regulation of glutamine synthetase activity by phosphorylation instead of by adenylylation. | o-phosphotyrosyl glutamine synthetase (p-gs) was isolated from highly adenylated glutamine synthetase (amp-gs) purified from mycobacterium phlei, by treatment with micrococcal nuclease. the physical characteristics of p-gs were quite similar to those of amp-gs except for the uv-absorption spectrum. in either mg2+- or mn2+-dependent biosynthetic reactions, the kinetic properties, such as optimum ph, vmax, and apparent km for each of three substrates of p-gs, were found to be in good agreement wit ... | 1988 | 2902854 |
| septic arthritis due to mycobacterium phlei presenting as infantile reiter's syndrome. | we describe a 7-year-old peruvian boy who presented with a 2 week history of conjunctivitis, urethritis and arthritis, in whom mycobacterium phlei was the only organism repeatedly isolated from synovial fluid and tissue and who responded to conventional antituberculous therapy. to our knowledge this is the first documented case of human disease caused by this microorganism. | 1989 | 2810265 |
| mycolic acid metabolic filiation and location in mycobacterium aurum and mycobacterium phlei. | synchronous cultures of mycobacterium aurum were used to prove a close relationship between cellular division and active synthesis of mycolic acids (characteristic long-chain 3-hydroxyacids, branched at position 2), confirming previous proposals. mycolic acid biosynthesis was studied in two species (mycobacterium phlei and m. aurum) each producing three types of mycolic acids: di-unsatured mycolates, oxomycolates and wax-ester mycolates (ester of dicarboxymycolic acid and 2-icosanol or 2-octadec ... | 1989 | 2714297 |
| trehalose dimycolate enhances resistance to infection in neutropenic animals. | bacterial infections are lethal complications of neutropenia, and antibiotics alone are inadequate therapy for these infections. irradiated mice become severely neutropenic and remain susceptible to infection for 2 to 3 weeks, depending on the dose and quality of radiation. some bacterial cell wall derivatives stimulate nonspecific host defense mechanisms against a variety of microbes which might cause postirradiation infection. in this study we determined if the cell wall glycolipid trehalose d ... | 1989 | 2663726 |
| physical and chemical characterization of glutamine synthetase purified from mycobacterium phlei. | glutamine synthetase (l-glutamate: ammonia ligase [adp forming]) [ec 6.3.1.2] has been purified from a gram-positive, acid-fast bacterium, mycobacterium phlei, by simple procedures with 57% recovery. the enzyme resembled that from mycobacterium smegmatis in the subunit size (56,000), molecular weight (670,000), amino acid composition, the amino acid sequence of the nh2-terminal, and the secondary structure. the enzyme activity was regulated by adenylylation of each subunit in the dodecameric mol ... | 1989 | 2569461 |
| nonspecific immunoprotection against rous sarcoma tumor in chicks. | an enzyme treated preparation of saprophytic mycobacterium phlei, referred as nsi, when administered intramuscularly has been found to protect the chicks against rous sarcoma virus induced tumor. a protection level of 35.4%, 24.1% and 21.2% were observed when challenged on 10th, 20th and 30th day post nsi inoculation. the tumor growth inhibitory-activity of nsi was significant (p less than 0.01). both, systemic and intralesional administration of nsi exhibited significant tumorostatic activity ( ... | 1989 | 2555299 |
| in vitro antibacterial effect of the essential oil of thymus longiflorus boiss. | the essential oil of thymus longiflorus boiss was tested for its in vitro antibacterial activity. the results showed antibacterial effects against gram-positive and gram-negative bacteria, especially against pseudomonas fluorescens and mycobacterium phlei. | 1989 | 2514339 |
| comparison of dot immunobinding assay, enzyme-linked immunosorbent assay and immunodiffusion for serodiagnosis of paratuberculosis. | a rapid, simple and inexpensive dot immunobinding assay (dia) was evaluated for the serodiagnosis of paratuberculosis in cattle. the assay was performed on nitrocellulose strips which were dotted with purified protoplasmic antigen of mycobacterium paratuberculosis. after incubation with test serum samples, the bound antibodies were detected using an enzyme-amplified immunostaining procedure. the efficacy of dia as a screening test for paratuberculosis was compared to that of an enzyme-linked imm ... | 1989 | 2512004 |
| induction of cell mediated immune response by nonspecific stimulator of immunity against antigenically unrelated oncogenic virus (marek's disease virus). | an enzyme treated preparation of mycobacterium phlei (nsi), induced strong cell mediated immune response (cmir) against specific as well as against nonspecific oncogenic marek's disease (mdv) in birds, as evinced by lymphocyte migration inhibition, (lmit) lymphocyte transformation test (lt) and lymphokine (lymphocyte migration inhibition factor) lyif assay. maximum cmir could be observed towards third week post inoculation. all the three tests exhibited a positive correlation. such phenomenon of ... | 1989 | 2479599 |
| [analysis of genes for ribosomal rna in mycobacterium phlei]. | 1988 | 2452272 | |
| step-wise isolation of rna and dna from mycobacteria. | mycobacterium phlei and m. smegmatis were lysed at -15 degrees c in the presence of guanidinium salts and poly(a)+-rna. nonpolyadenylated rna (mainly rrna) and dna were isolated in successive steps from the same lysate. dna isolated by this procedure was sufficient to yield distinct bands on treatment with restriction endonucleases as shown by hybridization to 125i-labeled rrna. the successive isolation of poly(a)+-rna, nonpolyadenylated rna, and high molecular weight dna from the same lysate by ... | 1986 | 2427627 |
| selective enzyme staining procedures for characterization of mycobacterial immunoprecipitates. | selective staining procedures for four different enzymes (malate dehydrogenase, glutamic oxaloacetic transaminase, leucine aminopeptidase and glucose phosphate isomerase) in combination with two-dimensional immunoelectrophoresis were successfully applied to the analysis of antigen preparations from mycobacteria. thus, a precipitate corresponding to malate dehydrogenase could e.g. be demonstrated in multi-linear precipitation patterns of mycobacterium avium, mycobacterium bovis bcg, mycobacterium ... | 1986 | 2417958 |