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vector potential of blattella germanica (l.) (dictyoptera: blattidae) for medically important bacteria at food handling establishments in jimma town, southwest ethiopia.cockroaches have been regarded as possible vectors of human enteropathogens. their presence and crawl particularly in food handling establishments could be risky for human health. therefore, this study was done to determine the vector potential of cockroach for medically important bacterial pathogens in restaurants and cafeterias. a cross-sectional study was conducted on cockroaches from restaurants and cafeterias in jimma town from may to september 2014. standard taxonomic keys and microbiologi ...201627294115
innovative bionanocomposite films of edible proteins containing liposome-encapsulated nisin and halloysite nanoclay.films and coatings based on natural polymers have gained increased interest for food packaging applications. in this work, halloysite and phosphatidylcholine liposomes encapsulating nisin were used to develop nanocomposite films of gelatin and casein. liposomes prepared with either soybean lecithin or phospholipon(®) showed particle size ranging from 124 to 178nm and high entrapment efficiency (94-100%). considering their stability, phospholipon(®) liposomes with 1.0mg/ml nisin were selected for ...201627289315
rational design, synthesis, anti-hiv-1 rt and antimicrobial activity of novel 3-(6-methoxy-3,4-dihydroquinolin-1(2h)-yl)-1-(piperazin-1-yl)propan-1-one derivatives.in the present study, fifteen novel 3-(6-methoxy-3,4-dihydroquinolin-1(2h)-yl)-1-(piperazin-1-yl)propan-1-one (6a-o) derivatives were designed as inhibitor of hiv-1 rt using ligand based drug design approach and in-silico evaluated for drug-likeness properties. designed compounds were synthesized, characterized and in-vitro evaluated for rt inhibitory activity against wild hiv-1 rt strain. among the tested compounds, four compounds (6a, 6b, 6j and 6o) exhibited significant inhibition of hiv-1 rt ...201627288643
mob/orit, a mobilizable site-specific recombination system for unmarked genetic manipulation in bacillus thuringiensis and bacillus cereus.bacillus thuringiensis and bacillus cereus are two important species in b. cereus group. the intensive study of these strains at the molecular level and construction of genetically modified bacteria requires the development of efficient genetic tools. to insert genes into or delete genes from bacterial chromosomes, marker-less manipulation methods were employed.201627286821
cxcl1, but not il-6, significantly impacts intraocular inflammation during infection.during intraocular bacterial infections, the primary innate responders are neutrophils, which may cause bystander damage to the retina or perturb the clarity of the visual axis. we hypothesized that cytokine il-6 and chemokine cxcl1 contributed to rapid neutrophil recruitment during bacillus cereus endophthalmitis, a severe form of intraocular infection that is characterized by explosive inflammation and retinal damage that often leads to rapid vision loss. to test this hypothesis, we compared e ...201627286792
whole-genome sequences of seven strains of bacillus cereus isolated from foodstuff or poisoning incidents.we present here the whole shotgun genome sequences of seven strains of bacillus cereus isolated from foodstuff samples or food poisoning incidents.201627284145
kinetic study of the active site structure of β-amylase from bacillus cereus var. mycoides.the subsite affinities of the active site of β-amylase from bacillus cereus var. mycoides were evaluated based on hiromi's theory, using (14)c-radiolabeled maltooligosaccharides as substrate. it was estimated that the active site consisted of six subsites, and all subsite affinities could be evaluated. the active site had a common subsite arrangement with those of β -amylases from soybean and wheat bran. the intrinsic breakdown rate constant of α-1,4 glucosidic linkage (kint) was five to seven t ...199627281142
time to stop holding the elevator: a new piece of the transport protein mechanism puzzle.in this issue of structure, mccoy et al. (2016) describe the 2.55-å x-ray structure of the outward-facing occluded conformation of the bacillus cereus maltose transporter malt. this structure represents the penultimate piece needed to complete the picture of the transport cycle of the glucose superfamily of membrane-spanning eiic components.201627276425
evaluation of the toxicity and toxicokinetics of cereulide from an emetic bacillus cereus strain of milk origin.bacillus cereus is an opportunistic foodborne agent causing food poisoning and many infectious diseases. the heat-stable emetic toxin cereulide is one of the most prevalent toxins produced by pathogenic b. cereus, resulting in symptoms such as emesis and liver failure. in the present work, the toxicity and toxicokinetics of cereulide from an emetic b. cereus isolate (cau45) of raw milk were evaluated. the production of cereulide was tested by a cytotoxicity test and enzyme immunoassay, and confi ...201627275834
linking bacillus cereus genotypes and carbohydrate utilization capacity.we characterised carbohydrate utilisation of 20 newly sequenced bacillus cereus strains isolated from food products and food processing environments and two laboratory strains, b. cereus atcc 10987 and b. cereus atcc 14579. subsequently, genome sequences of these strains were analysed together with 11 additional b. cereus reference genomes to provide an overview of the different types of carbohydrate transporters and utilization systems found in b. cereus strains. the combined application of api ...201627272929
the impact of oxygen on bacterial enteric pathogens.bacterial enteric pathogens are responsible for a tremendous amount of foodborne illnesses every year through the consumption of contaminated food products. during their transit from contaminated food sources to the host gastrointestinal tract, these pathogens are exposed and must adapt to fluctuating oxygen levels to successfully colonize the host and cause diseases. however, the majority of enteric infection research has been conducted under aerobic conditions. to raise awareness of the import ...201627261784
biochemical characterization and antimicrobial and antifungal activity of two endemic varieties of garlic (allium sativum l.) of the campania region, southern italy.extracts of the bulbs of the two endemic varieties "rosato" and "caposele" of allium sativum of the campania region, southern italy, were analyzed. the phenolic content, ascorbic acid, allicin content, and in vitro antimicrobial and antifungal activity were determined. ultra performance liquid chromatography with diode array detector performed polyphenol profile. the polyphenolic extracts showed antioxidant activity (ec50) lower than 120 mg. the amount of ascorbic acid and allicin in the two ext ...201627259073
anthrax toxin-expressing bacillus cereus isolated from an anthrax-like eschar.bacillus cereus isolates have been described harboring bacillus anthracis toxin genes, most notably b. cereus g9241, and capable of causing severe and fatal pneumonias. this report describes the characterization of a b. cereus isolate, bcfl2013, associated with a naturally occurring cutaneous lesion resembling an anthrax eschar. similar to g9241, bcfl2013 is positive for the b. anthracis pxo1 toxin genes, has a multi-locus sequence type of 78, and a paga sequence type of 9. whole genome sequenci ...201627257909
elucidation of enterotoxigenic bacillus cereus outbreaks in austria by complementary epidemiological and microbiological investigations, 2013.identifying bacillus cereus as the causative agent of a foodborne outbreak still poses a challenge. we report on the epidemiological and microbiological investigation of three outbreaks of food poisoning (a, b, and c) in austria in 2013. a total of 44% among 32 hotel guests (a), 22% among 63 employees (b) and 29% among 362 residents of a rehab clinic (c) fell sick immediately after meal consumption. b. cereus isolated from left overs or retained samples from related foods were characterized by t ...201627257745
draft genome sequence of bacillus cereus 905, a plant growth-promoting rhizobacterium of wheat.bacillus cereus 905 is a plant growth-promoting rhizobacterium, isolated from wheat rhizosphere. the draft genome sequence of this strain is 5.39 mb and harbors 5,412 coding sequences.201627257205
draft whole-genome sequences of 11 bacillus cereus food isolates.bacillus cereus is a foodborne pathogen causing emetic and diarrheal-type syndromes. here, we report the whole-genome sequences of 11 b. cereus food isolates.201627257202
comparative bioinformatics and experimental analysis of the intergenic regulatory regions of bacillus cereus hbl and nhe enterotoxin operons and the impact of cody on virulence heterogeneity.bacillus cereus is a food contaminant with greatly varying enteropathogenic potential. almost all known strains harbor the genes for at least one of the three enterotoxins nhe, hbl, and cytk. while some strains show no cytotoxicity, others have caused outbreaks, in rare cases even with lethal outcome. the reason for these differences in cytotoxicity is unknown. to gain insight into the origin of enterotoxin expression heterogeneity in different strains, the architecture and role of 5' intergenic ...201627252687
medicinal attributes of major phenylpropanoids present in cinnamon.excessive production of free radicals has been implicated in many diseases including cancer. they are highly reactive and bring about oxidation of biomolecules i.e., proteins, lipids and nucleic acids which are associated with many degenerative diseases. natural products acting as antioxidants have ability to neutralize free radicals and their actions and hence they mitigate their harmful effects. the present study was designed to investigate pharmacological properties viz., antioxidant, antibac ...201627245453
probiotic bacillus cereus strains, a potential risk for public health in china.bacillus cereus is an important cause of foodborne infectious disease and food poisoning. however, b. cereus has also been used as a probiotic in human medicine and livestock production, with low standards of safety assessment. in this study, we evaluated the safety of 15 commercial probiotic b. cereus preparations from china in terms of mislabeling, toxin production, and transferable antimicrobial resistance. most preparations were incorrectly labeled, as they contained additional bacterial spe ...201627242738
bacillus cereus ar156 extracellular polysaccharides served as a novel micro-associated molecular pattern to induced systemic immunity to pst dc3000 in arabidopsis.non-host resistance (nhr) is a broad-spectrum plant defense. upon colonizing on the surface on the root or leaves of non-host species, pathogens initial encounter preform and induce defense response in plant, such as induced hypersensitive response, pamps triggered immunity (pti), and effector triggered immunity (eti). the ability of plants to develop an induced systemic response (isr) in reaction to the colonization by non-pathogenic rhizobacterium depends on interactions between host plants an ...201627242694
contamination of healthcare workers' hands with bacterial spores.clostridium species and bacillus spp. are spore-forming bacteria that cause hospital infections. the spores from these bacteria are transmitted from patient to patient via healthcare workers' hands. although alcohol-based hand rubbing is an important hand hygiene practice, it is ineffective against bacterial spores. therefore, healthcare workers should wash their hands with soap when they are contaminated with spores. however, the extent of health care worker hand contamination remains unclear. ...201627236515
the role of active site flexible loops in catalysis and of zinc in conformational stability of bacillus cereus 569/h/9 β-lactamase.metallo-β-lactamases catalyze the hydrolysis of most β-lactam antibiotics and hence represent a major clinical concern. the development of inhibitors for these enzymes is complicated by the diversity and flexibility of their substrate-binding sites, motivating research into their structure and function. in this study, we examined the conformational properties of the bacillus cereus β-lactamase ii in the presence of chemical denaturants using a variety of biochemical and biophysical techniques. t ...201627235401
suitability of monotypic and mixed diets for anopheles hermsi larval development.the developmental time and survival to eclosion of anopheles hermsi barr & guptavanij fed monotypic and mixed diets of ten food types were examined in laboratory studies. larvae fed monotypic diets containing animal detritus (freeze-dried rotifers, freeze-dried daphnia pulicaria, and tetramin® fish food flakes) and the mixotrophic protistan cryptomonas ovata developed faster and survived better than larvae that were fed other monotypic diets. survival to adulthood of larvae fed several concentra ...201627232128
central venous access device-related bacillus cereus endocarditis: a case report and review of the literature.bacillus cereus typically presents as a gastrointestinal infection, but rarely manifests as systemic disease. this report describes a case of b. cereus-related endocarditis that presented as a sickle cell crisis and bacteremia. initial clinical suspicion was for laboratory contamination of blood cultures. the case herein described is intended to demonstrate an uncommon presentation of b. cereus infection and highlights the value of an aggressive need to further investigate and interpret unexpect ...201627231118
occurrence and behavior of bacillus cereus in naturally contaminated ricotta salata cheese during refrigerated storage.the present study shows the fate of bacillus cereus in refrigerated ricotta salata cheese during shelf-life. 144 ricotta salata cheese belonging to nine naturally contaminated batches were stored refrigerated and analyzed at 24 h, 30, 60 and 90 days of storage. total bacterial count, b. cereus spores and vegetative forms, intrinsic properties and composition were determined. the presence of spores was sporadic while the prevalence and the level of b. cereus vegetative cells decreased respectivel ...201627217369
destruction of bacillus cereus spores in a thick soy bean paste (doenjang) by continuous ohmic heating with five sequential electrodes.this study selected spores from bacillus cereus fsp-2 strain (the isolate from a commercial doenjang processing line) as the test strain which showed significantly higher thermal resistance (p < 0·05) than b. cereus reference strain (atcc 27348). the spores in doenjang were subjected to ohmic heating (oh) at 95, 105, 115 and 125°c for 30, 60 or 90 s using a five sequential electrode system (electrical field: 26·7 v cm(-1) ; alternating current frequency: 25 khz). oh at 105°c for 30-90 s reduced ...201627214292
application of volcanic ash particles for protein affinity purification with a minimized silica-binding tag.we recently reported that the spore coat protein, cotb1 (171 amino acids), from bacillus cereus mediates silica biomineralization and that the polycationic c-terminal sequence of cotb1 (14 amino acids), designated cotb1p, serves as a silica-binding tag when fused to other proteins. here, we reduced the length of this silica-binding tag to only seven amino acids (sb7 tag: rqssrgr) while retaining its affinity for silica. alanine scanning mutagenesis indicated that the three arginine residues in t ...201627212265
detection and quantification of bacillus cereus group in milk by droplet digital pcr.droplet digital pcr (ddpcr) is one of the newest and most promising methods for the detection and quantification of molecular targets by pcr. here, we optimized and used a new ddpcr assay for the detection and quantification of the bacillus cereus group in milk. we also compared the ddpcr to a standard qpcr assay. the new ddpcr assay showed a similar coefficient of determination and a better limit of detection compared to the qpcr assay during quantification of the target molecules in the sample ...201627211508
phytosynthesis of silver nanoparticles using artemisia marschalliana sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties.a rapid phytosynthesis of silver nanoparticles (agnps) using an extract from the aerial parts of artemisia marschalliana sprengel was investigated in this study. the synthesized agnps using a. marschalliana extract was analyzed by uv-visible spectroscopy, x-ray diffraction, and fourier transform infrared spectroscopy and further characterized by transmission electron microscopy, scanning electron microscopy, zeta potential, and energy-dispersive spectroscopy. characteristic absorption bands of a ...201627199558
three important amino acids control the regioselectivity of flavonoid glucosidation in glycosyltransferase-1 from bacillus cereus.glycosyltransferase-1 from bacillus cereus (bcgt1) catalyzes a reaction that transfers a glucosyl moiety to flavonoids, such as quercetin, kaempferol, and myricetin. the enzymatic glucosidation shows a broad substrate specificity when the reaction is catalyzed by wild-type bcgt1. preliminary assays demonstrated that the f240a mutant significantly improves the regioselectivity of enzymatic glucosidation toward quercetin. to unveil and further to control the catalytic function of bcgt1, mutation o ...201627198725
maldi-tof ms portrait of emetic and non-emetic bacillus cereus group members.the number of foodborne intoxications caused by emetic bacillus cereus isolates has increased significantly. as such, rapid and reliable methods to identify emetic strains appear to be clinically relevant. in this study, intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) was used to differentiate emetic and non-emetic bacilli. the phyloproteomic clustering of 34 b. cereus emetic and 88 non-emetic isolates classified as b. cereus, bacillus thur ...201627196540
successful treatment of bacillus cereus bacteremia in a patient with propionic acidemia.bacillus cereus can cause serious, life-threatening, systemic infections in immunocompromised patients. the ability of microorganism to form biofilm on biomedical devices can be responsible for catheter-related bloodstream infections. other manifestations of severe disease are meningitis, endocarditis, osteomyelitis, and surgical and traumatic wound infections. the most common feature in true bacteremia caused by bacillus is the presence of an intravascular catheter. herein, we report a case of ...201627195164
characterization of bacillus cereus isolates from local dairy farms in china.bacillus cereus is an important opportunistic foodborne pathogen. in the present work, a total of 306 milk and environmental samples were collected from 10 local dairy farms in beijing, china. of the 92 b. cereus-like isolates, 88 and 4 belonged to b. cereus and b. thuringiensis, respectively. the prevalence of b. cereus isolates in bedding, feces, feed, liquid manure and raw milk was 93.3%, 78.9%, 41.2%, 100.0% and 9.8%, respectively. three main toxin genes nhe, hbl and ces were detected with r ...201627190168
characterization of lyspbc4, a novel bacillus cereus-specific endolysin of bacteriophage pbc4.bacillus cereus is a spore-forming, gram-positive bacterium and is a major food-borne pathogen. a b. cereus-specific bacteriophage pbc4 was isolated from the soil of a stock farm, and its genome was analyzed. pbc4 belongs to the siphoviridae family and has a genome consisting of 80 647-bp-long double-stranded dna, including 123 genes and two trnas. lyspbc4, the endolysin of pbc4, has an enzymatically active domain (ead) on its n-terminal region and a putative cell wall-binding domain (cbd) on it ...201627190165
the global regulator cody is required for the fitness of bacillus cereus in various laboratory media and certain beverages.the impact of gene mutations on the growth of the cells can be studied using pure cultures. however, the importance of certain proteins and pathways can be also examined via co-culturing wild type and its mutant derivative. here, the relative fitness of a mutant strain that lacks the global nitrogen regulator, cody, was examined in bacillus cereus, a food poisoning gram-positive bacterium. fitness measurements revealed that the δcody strain was outcompeted when cocultured with the wild-type atcc ...201627190142
the two-component signal transduction system yvcpq regulates the bacterial resistance to bacitracin in bacillus thuringiensis.yvcpq is one of the two-component signal transduction systems that respond to specific stimuli and enable cells to adjust multiple cellular functions. it consists of a histidine kinase yvcq and a response regulator yvcp. in this study, through searching the consensus sequence recognized by yvcp, we found four yvcp-binding motifs in the promoter regions of genes yvcr (bmb171_c4100), bmb171_c4385, kapd (bmb171_c4525) and bmb171_c4835 in bacillus thuringiensis bmb171 which is a representative of ba ...201627188294
the lipopeptide 6-2 produced by bacillus amyloliquefaciens anti-ca has potent activity against the biofilm-forming organisms.both the whole cells and protoplasts of pseudomonas aeruginosa pao1 and bacillus cereus, two biofilm-forming bacteria, were disrupted by the lipopeptide 6-2 produced by bacillus amyloliquefaciens anti-ca. the lipopeptide 6-2 could also effectively inhibit the formation of biofilms and disperse pre-formed biofilms. live/dead staining of the biofilms grown in the absence or presence of the lipopeptide 6-2 showed that more dead bacterial cells in the presence of the lipopeptide than those in the ab ...201627184127
label-free bimodal waveguide immunosensor for rapid diagnosis of bacterial infections in cirrhotic patients.spontaneous bacterial peritonitis is an acute bacterial infection of ascitic fluid; it has a high incidence in cirrhotic patients and it is associated with high mortality. in such a situation, early diagnosis and treatment is crucial for the survival of the patient. however, bacterial analysis in ascitic fluid is currently based on culture methods, which are time-consuming and laborious. we report here the application of a photonic interferometer biosensor based on a bimodal waveguide (bimw) for ...201627183281
antibacterial, antifungal, phytotoxic, antioxidant and hemagglutination activities of organic fractions of arisaema tortuosum.in the current study, the antimicrobial, phytotoxic, haemagglutination and antioxidant potential of crude methanolic extract (crd. meoh ext.) and four organic fractions of arisaema tortuosum was investigated. all fractions have been screened for antimicrobial properties against eight bacterial pathogens and six fungal pathogens using agar well diffusion and tube dilution method, respectively. furthermore, the organic fractions were also screened for its phytotoxicity against lemna minor. haemagg ...201627166544
plantazolicin is an ultra-narrow spectrum antibiotic that targets the bacillus anthracis membrane.plantazolicin (pzn) is a ribosomally synthesized and post-translationally modified natural product from bacillus methylotrophicus fzb42 and bacillus pumilus. extensive tailoring to twelve of the fourteen amino acid residues in the mature natural product endows pzn with not only a rigid, polyheterocyclic structure, but also antibacterial activity. here we report a remarkably discriminatory activity of pzn toward bacillus anthracis, which rivals a previously-described gamma (γ) phage lysis assay i ...201627152321
coumarin derivatives as adjuvants: from in silico physicochemical characterization to in vitro evaluation against gram positive bacteria.a series of acylated coumarin derivatives have been evaluated for their in silico admet properties and in vitro antibacterial activities.201627151485
synthesis, anti-inflammatory and antibacterial activities of novel pyrazolo[4,3-g]pteridines.a novel series of 6-substituted pyrazolo[3,4-g]pteridines 2-9 and pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pteridin-2(3h)-one (thione) 10 and 11 was synthesized using the starting compound 3,7-dimethyl-1-phenylpyrazolo[4',3':5,6]pyrazino[2,3-d][1,3]oxazin-5(1h)-one 2. the structure of the newly synthesized compounds was elucidated by ir, (1)h-nmr, (13)c-nmr, mass spectroscopy and elemental analyses. the anti-inflammatory activity of all the newly synthesized compounds was evaluated using the carrage ...201627150479
pulmonary illness as a consequence of occupational exposure to shrimp shell powder.an employee with no prior history of allergy or asthma, experienced respiratory and flu-like symptoms during production of shrimp shell powder in a seafood savory factory in norway. we aimed to clarify the diagnosis and to identify the cause of the symptoms by specific inhalation challenge (sic) and by characterizing the powder's biocontaminants, particle size fractions and inflammatory potential.201627148672
in vitro evaluation of the antioxidant, 3,5-dihydroxy-4-ethyl-trans-stilbene (dets) isolated from bacillus cereus as a potent candidate against malignant melanoma.3,5-dihydroxy q1 -4-ethyl-trans-stilbene (dets) is a natural stilbene, which was first identified as bioactive bacterial secondary metabolite isolated from bacillus cereus associated with a rhabditid entomopathogenic nematode. the present study was intended to investigate the antioxidant and anticancer activity of this compound in vitro. antioxidant activity was investigated by assaying dpph free radical scavenging, superoxide radical-(o2..) scavenging, hydroxyl radical scavenging and metal chel ...201627148169
biosynthesis of silver nanoparticles by novosphingobium sp. thg-c3 and their antimicrobial potential.the present study described biosynthesis of silver nanoparticles (agnps) using a bacterial strain novosphingobium sp. thg-c3, isolated from soil, and their application in antibacterial activity. the maximum absorbance values of the synthesized agnps was measured at 406 nm in ultraviolet-visible spectrophotometry and were mostly spherical in shape with particle size in range of 8-25 nm by field emission transmission electron microscopy analysis. x-ray diffraction pattern corresponding to planes ( ...201727145847
microbiome associations in pigs with the best and worst clinical outcomes following co-infection with porcine reproductive and respiratory syndrome virus (prrsv) and porcine circovirus type 2 (pcv2).on a world-wide basis, co-infections involving porcine reproductive and respiratory syndrome virus (prrsv) and porcine circovirus type 2 (pcv2) are common and contribute to a range of polymicrobial disease syndromes in swine. both viruses compromise host defenses, resulting in increased susceptibility to infections by primary and secondary pathogens that can affect growth performance as well as increased morbidity and mortality. an experimental population of 95 pigs was co-infected with prrsv an ...201627139023
preparation and biological activity of new collagen composites, part i: collagen/zinc titanate nanocomposites.the aim of this investigation was to develop new antimicrobial collagen/zinc titanate (zntio3) biomaterials using a sol-gel cryogenic draying technology in keeping the native collagen activity. broad-spectrum antimicrobial activity was demonstrated against firmicutes (staphylococcus epidermidis, bacillus cereus, and candida lusitaniae) and gracilicutes (escherichia coli, salmonella enterica, and pseudomonas putida) microorganisms. the antimicrobial activity as well as the cytotoxicity were speci ...201627138724
survey of the antibiofilm and antimicrobial effects of zingiber officinale (in vitro study).candidiasis is one of the most prevalent and important opportunistic fungal infections of the oral cavity caused by candida yeast species like candida albicans, c. glabrata, and c. krusei. in addition, several bacteria can cause oral infections. the inhibition of microbial biofilm is the best way to prevent oral infections.201627127591
structural and biochemical characterization of the bacillus cereus 3-hydroxyisobutyrate dehydrogenase.the 3-hydroxyisobutyrate dehydrogenase (hibadh) family catalyzes the nad(+)- or nadp(+)-dependent oxidation of various β-hydroxyacid substrates into their cognate semialdehydes for diverse metabolic pathways. because hibadh group members exhibit different substrate specificities, the substrate-recognition mode of each enzyme should be individually characterized. in the current study, we report the biochemical and structural analysis of a hibadh group enzyme from bacillus cereus (bchibadh). bchib ...201627120461
relapsing peritonitis with bacillus cereus in a patient on continuous ambulatory peritoneal dialysis.we present a case where bacillus cereus was determined to be the causative agent of relapsing peritonitis in a patient on continuous ambulatory peritoneal dialysis (capd). the patient, a 70-year-old man from the faroe islands, was admitted with relapsing peritonitis four times over a 3-month period. peritoneal cultures were positive for growth of b. cereus, a rare bacterial cause of peritonitis. the cultures demonstrated susceptibility to vancomycin, and therefore the patient was treated with in ...201627118739
cyclic diguanylate regulation of bacillus cereus group biofilm formation.biofilm formation can be considered a bacterial virulence mechanism. in a range of gram-negatives, increased levels of the second messenger cyclic diguanylate (c-di-gmp) promotes biofilm formation and reduces motility. other bacterial processes known to be regulated by c-di-gmp include cell division, differentiation and virulence. among gram-positive bacteria, where the function of c-di-gmp signalling is less well characterized, c-di-gmp was reported to regulate swarming motility in bacillus sub ...201627116468
culture-dependent and -independent methods to investigate the predominant microorganisms associated with wet processed coffee.the fermentation process of yunnan arabica coffee is a typical wet fermentation. its excellent quality is closely related to microbes in the process of fermentation. the purpose of this study was to isolate and identify the microorganisms in the wet method of coffee processing in yunnan province, china. microbial community structure and dominant bacterial species were evaluated by traditional cultivated separation method and pcr-dgge technology, and were further analyzed in combination with the ...201627113591
potential antimicrobial and antiproliferative activities of autochthonous starter cultures and protease epg222 in dry-fermented sausages.this work studied the presence of nitrogen compounds with bioactive properties in iberian pork sausages that were manufactured using different autochthonous starter cultures (pediococcus acidilactici ms200 and staphylococcus vitulus rs34) and protease epg222. nitrogen compounds were extracted and evaluated for their antimicrobial effect against spoilage and pathogenic bacteria, such as bacillus cereus, escherichia coli, salmonella choleraesuis, staphylococcus aureus and listeria monocytogenes, a ...201627112426
optimization of pulsed-field gel electrophoresis procedure for bacillus cereus.in order to develop a rapid and reliable method for b. cereus genotyping, factors influencing pfge results, including preparation of bacterial cells embedded in agarose, lysis of embedded cells, enzymatic digestion of intact genomic dna, and electrophoresis parameters allowing for reproducible and meaningful dna fragment separation, were controlled. optimal cellular growth (luria-bertani agar plates for 12-18 h) and lysis conditions (4 h incubation with 500 µg/ml lysozyme) produced sharp bands on ...201627109136
alternative biotransformation of retinal to retinoic acid or retinol by an aldehyde dehydrogenase from bacillus cereus.a novel bacterial aldehyde dehydrogenase (aldh) that converts retinal to retinoic acid was first identified in bacillus cereus the amino acid sequence of aldh from b. cereus (bcaldh) was more closely related to mammalian aldhs than to bacterial aldhs. this enzyme converted not only small aldehydes to carboxylic acids but also the large aldehyde all-trans-retinal to all-trans-retinoic acid with nad(p)(+) we newly found that bcaldh and human aldh (aldh1a1) could reduce all-trans-retinal to all-tra ...201627107124
unicellular cyanobacteria synechocystis accommodate heterotrophic bacteria with varied enzymatic and metal resistance properties.the interactions between heterotrophic bacteria and primary producers have a profound impact on the functioning of marine ecosystem. we characterized the enzymatic and metal resistance properties of fourteen heterotrophic bacteria isolated from a unicellular cyanobacterium synechocystis sp. that came from a heavy metal contaminated region of cochin estuary, southwest coast of india. based on 16s rrna gene sequence similarities, the heterotrophic bacteria were grouped into three phyla: namely act ...201627106264
two distinct pathways lead bacillus thuringiensis to commit to sporulation in biofilm.the spore-forming bacterium bacillus thuringiensis is an efficient biofilm producer, responsible for persistent contamination of industrial food processing systems. b. thuringiensis biofilms are highly heterogeneous bacterial structures in which three distinct cell types controlled by quorum sensing regulators were identified: plcr-controlled virulent cells, nprr-dependent necrotrophic cells and cells committed to sporulation, a differentiation process controlled by rap phosphatases and spo0a-p. ...201627106256
genome sequence of bacillus cereus phage vb_bces-my192.italic! bacillus cereusis an opportunistic foodborne pathogen. the phage vb_bces-my192 was isolated from italic! b. cereus192 in a cooked rice sample. the temperate phage belongs to the italic! siphoviridaefamily, italic! caudoviralesorder. here we announce the phage genome sequence and its annotation, which may expand the understanding of italic! b. cereussiphophages.201627103733
chemical composition, antioxidant and antibacterial activities of essential oils from ferulago angulata.ferulago angulata boiss. (apiaceae), a perennial aromatic herb, grows wild in iran. the aerial parts of f. angulata are used as a flavouring in foods, especially dairy foods by indigenous people in western and southwestern iran.201627102982
diversity of indigenous endophytic bacteria associated with the roots of chinese cabbage (brassica campestris l.) cultivars and their antagonism towards pathogens.the study aimed to reveal the diversity of endophytic bacteria in the roots of chinese cabbage (cc) cultivated in two areas in korea, namely, seosang-gun (ss) and haenam-gun (hn), and also in a transgenic plant (tp) from the laboratory. a total of 653 colonies were isolated from the interior of cc roots, comprising 118, 302, and 233 isolates from ss, hn, and tp samples, respectively. based on 16s rrna gene sequence analysis, the isolates belonged to four major phylogenetic groups: high-g+c gram- ...201627095454
cleaning and disinfection of bacillus cereus biofilm.methodology has been evolving for the testing of disinfectants against bacterial single-species biofilms, as the difficulty of biofilm remediation continues to gain much-needed attention. bacterial single-species biofilm contamination presents a real risk to good manufacturing practice-regulated industries. however, mixed-species biofilms and biofilms containing bacterial spores remain an even greater challenge for cleaning and disinfection. among spore-forming microorganisms frequently encounte ...201627091884
microbiological and modeling approach to derive performance objectives for bacillus cereus group in ready-to-eat salads.in this article, the performance objectives (pos) for bacillus cereus group (bc) in celery, cheese, and spelt added as ingredients in a ready-to-eat mixed spelt salad, packaged under modified atmosphere, were calculated using a bayesian approach. in order to derive the pos, bc detection and enumeration were performed in nine lots of naturally contaminated ingredients and final product. moreover, the impact of specific production steps on the bc contamination was quantified. finally, a sampling p ...201627088886
increased biomass production by mesophilic food-associated bacteria through lowering the growth temperature from 30°c to 10°c.five isolates from chilled food and refrigerator inner surfaces and closely related reference strains of the species escherichia coli, listeria monocytogenes, staphylococcus xylosus, bacillus cereus, pedobacter nutrimenti, and pedobacter panaciterrae were tested for the effect of growth temperature (30°c and 10°c) on biomass formation. growth was monitored via optical density, and biomass formation was measured at the early stationary phase based on the following parameters in complex and define ...201627084015
different antibacterial activity of novel theophylline-based ionic liquids - growth kinetic and cytotoxicity studies.the aim of this study was to investigate novel theophylline-based ionic liquids and their cytotoxic effects towards model gram-positive and gram-negative bacteria (bacillus cereus and escherichia coli, respectively). growth kinetics, respiratory rates and dehydrogenase activities were studied in the presence of ionic liquids at concentrations ranging from 10 to 1000mg/l. additionally, the influence of ionic liquids on bacterial cells associated with specific interactions based on the structure o ...201627082812
discovery and characterization of bicereucin, an unusual d-amino acid-containing mixed two-component lantibiotic.lantibiotics are a group of ribosomally synthesized and post-translationally modified peptides (ripps) exhibiting antimicrobial activity. they are characterized by the presence of the thioether-containing bisamino acids lanthionine and methyllanthionine. here, we report a two-component lantibiotic from bacillus cereus sj1 with unusual structural features that we named bicereucin. unlike all previous two-component lantibiotics, only one of the two peptides of bicereucin contains a lanthionine. th ...201627074593
rhizosphere of avicennia marina (forsk.) vierh. as a landmark for polythene degrading bacteria.due to high durability, cheap cost, and ease of manufacture, 311 million tons of plastic-based products are manufactured around the globe per annum. the slow/least rate of plastic degradation leads to generation of million tons of plastic waste per annum, which is of great environmental concern. of the total plastic waste generated, polythene shared about 64 %. various methods are available in the literature to tackle with the plastic waste, and biodegradation is considered as the most accepted, ...201627072028
effect of surfactants and inducers on increased uricase production under submerged fermentations by bacillus cereus.uricase is a clinical enzyme used for the oxidation of uric acid crystals in gout disease. the present study aimed to increase the suitable surfactant-mediated uricase production on induction by different concentrations of inducers. the efficiency of bacillus cereus to produce extracellular uricase enzyme was studied in uric acid-containing agar plates. among the studied inducers, uric acid is the potential inducer for uricase production under submerged fermentations (smf), which induced 19.41 u ...201727071031
rhizospheric bacteria of maize with potential for biocontrol of fusarium verticillioides.the stalk, ear and root rot (serr) of maize caused by fusarium verticillioides (fv) severely impacts crop production in tropical and subtropical regions. the aim of the present work was to screen bacterial isolates in order to find novel native biocontrol agents against fv. a culturable bacterial collection consisting of 11,520 isolates enriched in firmicutes and proteobacteria was created from rhizosphere samples taken from serr symptomatic or asymptomatic maize plants. the complete collection ...201627066355
synthesis and biological evaluation of novel structural hybrids of benzofuroxan derivatives and fluoroquinolones.a series of novel hybrids based on benzofuroxan derivatives and fluoroquinolones (4a-d-6a-d) have been synthesized. unexpectedly, the reactions have resulted in salt products formation during the hydrolysis of benzofuroxans by water molecules being present in the solvent instead of usual substitution products. all the compounds have been screened for antimicrobial and toxic activities. all resulting compounds retain high activity characteristic for fluoroquinolones. many of the salts based on be ...201627061980
bacillus cereus response to a proanthocyanidin trimer, a transcriptional and functional analysis.proanthocyanidins are abundant in peanut skin, and in this study, the antibacterial effects of a peanut skin extract (pse) against food-borne bacteria were investigated to find its minimum inhibitory concentration. food-borne gram-positive bacteria, and in particular bacillus cereus, was more sensitive to pse. in particular, the inhibitory activity of epicatechin-(4β → 6)-epicatechin-(2β → o→7, 4β → 8)-catechin (eec), a proanthocyanidin trimer from peanut skin, against b. cereus was stronger tha ...201627061585
occurrence of toxigenic bacillus cereus and bacillus thuringiensis in doenjang, a korean fermented soybean paste.this study determined the prevalence and toxin profile of bacillus cereus and bacillus thuringiensis in doenjang, a fermented soybean food, made using both traditional and commercial methods. the 51 doenjang samples tested were broadly contaminated with b. cereus; in contrast, only one sample was positive for b. thuringiensis. all b. cereus isolates from doenjang were positive for diarrheal toxin genes. the frequencies of nheabc and hblacd in traditional samples were 22.7 and 0%, respectively, w ...201627052865
microbial dynamics during shelf-life of industrial ricotta cheese and identification of a bacillus strain as a cause of a pink discolouration.dairy products are perishable and have to be preserved from spoilage during the food chain to achieve the desired shelf-life. ricotta is a typical italian soft dairy food produced by heat coagulation of whey proteins and is considered to be a light and healthy product. the shelf-life of ricotta could be extended, as required by the international food trade market; however, heat resistant microflora causes spoilage and poses issues regarding the safety of the product. next-generation sequencing ( ...201627052696
optimization of process parameters for the rapid biosynthesis of hematite nanoparticles.hematite (α-fe2o3) nanoparticles are widely used in various applications including gas sensors, pigments owing to its low cost, environmental friendliness, non-toxicity and high resistance to corrosion. these nanoparticles were generally synthesized by different chemical methods. in the present study, nanoparticles were synthesized rapidly without heat treatment by biosynthesis approach using culture supernatant of bacillus cereus svk1. the physiochemical parameters for rapid synthesis were opti ...201627045277
development of a powder formulation based on bacillus cereus sensu lato strain b25 spores for biological control of fusarium verticillioides in maize plants.maize is an economically important crop in northern mexico. different fungi cause ear and root rot in maize, including fusarium verticillioides (sacc.) nirenberg. crop management of this pathogen with chemical fungicides has been difficult. by contrast, the recent use of novel biocontrol strategies, such as seed bacterization with bacillus cereus sensu lato strain b25, has been effective in field trials. these approaches are not without their problems, since insufficient formulation technology, ...201627038945
isolation of aluminum-tolerant bacteria capable of nitrogen removal in activated sludge.four strains of bacteria capable of withstanding 20mm concentration of aluminum were isolated from activated sludge in a bioreactor. 16s rrna identification and morphological characteristics indicated that these strains were chryseobacterium sp. b1, brevundimonas diminuta b3, hydrogenophaga sp. b4, and bacillus cereus b5. phylogenetic analysis revealed the position and interrelationships of these bacteria. b. diminuta b3 and hydrogenophaga sp. b4 could achieve nitrate nitrogen removal of 94.0% a ...201627038879
impact of bacillus cereus nrkt on grape ripe rot disease through resveratrol synthesis in berry skin.vine growers are faced with the difficult problem of how to control grape ripe rot disease in vineyards because of fear of accumulation of pesticide residues on grape berries near harvest. biological control is an alternative non-hazardous technique to control the diseases.201727038426
molecular phylogeny of heterotrophic nitrifiers and aerobic denitrifiers and their potential role in ammonium removal.to investigate the physiology and taxonomic composition of the key players of nitrification and denitrification processes in paddy fields, culture dependent and independent studies have been carried out. a total of 28 bacterial strains have been screened in which six were capable of reducing nitrate and nitrite as well as having significant ammonium removal potential. 16s rrna-pcr-dgge-based molecular typing of enriched batch culture was done with time duration to explore and identify dominant a ...201627037833
hygienic characteristics and microbiological hazard identification in horse and donkey raw milk.today the interest toward horse (equus caballus) and donkey (equus asinus) milk for human consumption is receiving a renewed attention because of its particular composition, hypoallergenicity, and nutraceutical properties. the realistic perspective of global use of this aliment in balanced diets, especially for infancy and geriatrics, poses the need for a more in depth knowledge on milk hygiene and on the health status of dairy animals, as a prerequisite of consumers' safety. the aim of this pap ...201727033528
identification and pathogenic potential of clinical bacillus and paenibacillus isolates.the soil-related bacillus and paenibacillus species have increasingly been implicated in various human diseases. nevertheless, their identification still poses problems in the clinical microbiology laboratory and, with the exception of bacillus anthracis and bacillus cereus, little is known on their pathogenicity for humans. in this study, we evaluated the use of matrix-assisted laser desorption-ionization time of flight mass spectrometry (maldi-tof ms) in the identification of clinical isolates ...201627031639
noninvasive ph monitoring for bacterial detection in platelet concentrates.bacterial contamination of platelet concentrates (pcs) remains the prevalent posttransfusion infectious risk. the ph safe system, a noninvasive method used to measure ph of pc for quality control, was evaluated herein as a rapid method to detect bacterial contamination in pcs.201627028108
antibacterial properties and effects of fruit chilling and extract storage on antioxidant activity, total phenolic and anthocyanin content of four date palm (phoenix dactylifera) cultivars.phoenix dactylifera or date palm fruits are reported to contain natural compounds that exhibit antioxidant and antibacterial properties. this research aimed to study the effect of fruit chilling at 4 °c for 8 weeks, extract storage at -20 °c for 5 weeks, and extraction solvents (methanol or acetone) on total phenolic content (tpc), antioxidant activity and antibacterial properties of saudi arabian p. dactylifera cv mabroom, safawi and ajwa, as well as iranian p. dactylifera cv mariami. the stora ...201627023514
optimization of peptide nucleic acid fluorescence in situ hybridization (pna-fish) for the detection of bacteria: the effect of ph, dextran sulfate and probe concentration.fluorescence in situ hybridization (fish) is a molecular technique widely used for the detection and characterization of microbial populations. fish is affected by a wide variety of abiotic and biotic variables and the way they interact with each other. this is translated into a wide variability of fish procedures found in the literature. the aim of this work is to systematically study the effects of ph, dextran sulfate and probe concentration in the fish protocol, using a general peptide nuclei ...201627021959
adhesion and removal kinetics of bacillus cereus biofilms on ni-ptfe modified stainless steel.biofilm control remains a challenge to food safety. a well-studied non-fouling coating involves codeposition of polytetrafluoroethylene (ptfe) during electroless plating. this coating has been reported to reduce foulant build-up during pasteurization, but opportunities remain in demonstrating its efficacy in inhibiting biofilm formation. herein, the initial adhesion, biofilm formation, and removal kinetics of bacillus cereus on ni-ptfe-modified stainless steel (ss) are characterized. coatings lo ...201627020838
erratum to: seasonal trend and clinical presentation of bacillus cereus bloodstream infection: association with summer and indwelling catheter.bacillus cereus, an opportunistic pathogen, can cause fatal infection. however, b. cereus bloodstream infections (bsis) have not been well characterised. from 2008 to 2013, b. cereus isolates from all of the specimens and patients with b. cereus bsis were identified. environmental samples were collected to detect b. cereus contamination. we also characterised the clinical presentation of b. cereus bsi through analyses of risk factors for bsi and mortality. a total of 143 clinical b. cereus isola ...201627010814
identification of cereulide-producing bacillus cereus by nucleic acid chromatography and reverse transcription real-time pcr.rna extracts were analyzed with a nucleic acid sequence-based amplification (nasba) - nucleic acid chromatography and a reverse transcription-quantitative pcr assay (rt-qpcr) based on the taqman probe for identification of cereulide-producing bacillus cereus. all 100 emetic b. cereus strains were found to give positive results, but 50 diarrheal b. cereus strains and other bacterial species showed negative results in the nasba-chromatography. that is, the assay could selectively identify the emet ...201627009509
antibacterial activity and action mechanism of the essential oil from enteromorpha linza l. against foodborne pathogenic bacteria.foodborne illness and disease caused by foodborne pathogenic bacteria is continuing to increase day by day and it has become an important topic of concern among various food industries. many types of synthetic antibacterial agents have been used in food processing and food preservation; however, they are not safe and have resulted in various health-related issues. therefore, in the present study, essential oil from an edible seaweed, enteromorpha linza (aeo), was evaluated for its antibacterial ...201627007365
synthesis and evaluation of ester derivatives of 10-hydroxycanthin-6-one as potential antimicrobial agents.as part of our continuing research on canthin-6-one antimicrobial agents, a new series of ester derivatives of 10-hydroxycanthin-6-one were synthesized using a simple and effective synthetic route. the structure of each compound was characterized by nmr, esi-ms, ft-ir, uv, and elemental analysis. the antimicrobial activity of these compounds against three phytopathogenic fungi (alternaria solani, fusarium graminearum, and fusarium solani) and four bacteria (bacillus cereus, bacillus subtilis, ra ...201627007362
phytochemical analysis, antimicrobial and antioxidant activities of euphorbia golondrina l.c. wheeler (euphorbiaceae juss.): an unexplored medicinal herb reported from cameroon.this study aimed at determining the phytochemical constituents of euphorbia golondrina l.c. wheeler, an alien invasive medicinal herb that is used for the treatment of gastroenteritis related ailments, diabetes, conjunctivitis, gastritis, enterocolitis, tonsillitis, vaginitis, hemorrhoids, prostatism, warts and painful swellings by the mundani people of the mount bambouto caldera in southwestern cameroon, and to evaluate its in vitro antimicrobial and antioxidant activities. susceptibility testi ...201627006873
secreted compounds of the probiotic bacillus clausii strain o/c inhibit the cytotoxic effects induced by clostridium difficile and bacillus cereus toxins.although the use of probiotics based on bacillus strains to fight off intestinal pathogens and antibiotic-associated diarrhea is widespread, the mechanisms involved in producing their beneficial effects remain unclear. here, we studied the ability of compounds secreted by the probiotic bacillus clausii strain o/c to counteract the cytotoxic effects induced by toxins of two pathogens, clostridium difficile and bacillus cereus, by evaluating eukaryotic cell viability and expression of selected gen ...201627001810
evaluation of bacterial contamination of dental unit waterlines and use of a newly designed measurement device to assess retraction of a dental chair unit.dental unit waterline (duwl) output water is delivered through instruments of a dental chair unit (dcu) to irrigate and cool teeth. however, these waterlines can be heavily contaminated with bacteria.201627000421
the phylogeny of bacillus cereus sensu lato.the three main species of the bacillus cereus sensu lato, b. cereus, b. thuringiensis, and b. anthracis, were recognized and established by the early 1900 s because they each exhibited distinct phenotypic traits. b. thuringiensis isolates and their parasporal crystal proteins have long been established as a natural pesticide and insect pathogen. b. anthracis, the etiological agent for anthrax, was used by robert koch in the 19th century as a model to develop the germ theory of disease, and b. ce ...201626999390
shelf life of donkey milk subjected to different treatment and storage conditions.the aim of this study was to investigate the effects of different treatment conditions on microbiological indicators of donkey milk hygiene and their evolution during shelf life at 4 and 12°c from 3 to 30d, simulating a farm-scale pasteurization and packing system. four treatment conditions were tested: no treatment (raw milk), pasteurization (65°c × 30 min), high-pressure processing (hpp), and pasteurization plus hpp. the microbiological quality of the raw donkey milk investigated was not optim ...201626995124
extracellular synthesis of silver and gold nanoparticles by sporosarcina koreensis dc4 and their biological applications.the present study highlights the microbial synthesis of silver and gold nanoparticles by sporosarcina koreensis dc4 strain, in an efficient way. the synthesized nanoparticles were characterized by ultraviolet-visible spectrophotometry, which displayed maximum absorbance at 424nm and 531nm for silver and gold nanoparticles, respectively. the spherical shape of nanoparticles was characterized by field emission transmission electron microscopy. the energy dispersive x-ray spectroscopy and elemental ...201626992796
detection of carboxylesterase and esterase activity in culturable gut bacterial flora isolated from diamondback moth, plutella xylostella (linnaeus), from india and its possible role in indoxacarb degradation.diamondback moth (dbm), plutella xylostella (linnaeus), is a notorious pest of brassica crops worldwide and is resistant to all groups of insecticides. the insect system harbors diverse groups of microbiota, which in turn helps in enzymatic degradation of xenobiotic-like insecticides. the present study aimed to determine the diversity of gut microflora in dbm, quantify esterase activity and elucidate their possible role in degradation of indoxacarb. we screened 11 geographic populations of dbm i ...201626991291
purification and characterization of plantaricin jla-9: a novel bacteriocin against bacillus spp. produced by lactobacillus plantarum jla-9 from suan-tsai, a traditional chinese fermented cabbage.bacteriocins are ribosomally synthesized peptides with antimicrobial activity produced by numerous bacteria. a novel bacteriocin-producing strain, lactobacillus plantarum jla-9, isolated from suan-tsai, a traditional chinese fermented cabbage, was screened and identified by its physiobiochemical characteristics and 16s rdna sequence analysis. a new bacteriocin, designated plantaricin jla-9, was purified using butanol extraction, gel filtration, and reverse-phase high-performance liquid chromatog ...201626985692
formation and biopharmaceutical characterization of electrospun pvp mats with propolis and silver nanoparticles for fast releasing wound dressing.antibacterial, antiviral, antifungal, antioxidant, anti-inflammatory, and anticancer activities of propolis and its ability to stimulate the immune system and promote wound healing make it a proper component for wound dressing materials. silver nanoparticles are recognized to demonstrate strong antiseptic and antimicrobial activity; thus, it also could be considered in the development of products for wound healing. combining propolis and silver nanoparticles can result in improved characteristic ...201626981531
assessment of the bacterial contamination of hand air dryer in washrooms.the present study was carried out, using standard techniques, to identify and count the bacterial contamination of hand air dryers, used in washrooms. bacteria were isolated from the air flow, outlet nozzle of warm air dryers in fifteen air dryers used in these washrooms. bacteria were found to be relatively numerous in the air flows. bacterially contaminated air was found to be emitted whenever a warm air dryer was running, even when not being used for hand drying. our investigation shows that ...201626981009
development of a double-antibody sandwich elisa for rapid detection of bacillus cereus in food.bacillus cereus is increasingly recognized as one of the major causes of food poisoning in the industrialized world. in this paper, we describe a sensitive double-antibody sandwich enzyme-linked immunosorbent assay (elisa) that was developed for rapid detection of b. cereus in food to minimize the risk of contamination. the polyclonal antibody (pab) and monoclonal antibodies (mabs) specific to b. cereus were generated from rabbit antiserum and mouse ascites, respectively, using the octanoic acid ...201626976753
recombinant broad-range phospholipase c from listeria monocytogenes exhibits optimal activity at acidic ph.the broad-range phospholipase c (plc) from listeria monocytogenes has been expressed using an intein expression system and characterized. this zinc metalloenzyme, similar to the homologous enzyme from bacillus cereus, targets a wide range of lipid substrates. with monomeric substrates, the length of the hydrophobic acyl chain has significant impact on enzyme efficiency by affecting substrate affinity (km). based on a homology model of the enzyme to the b. cereus protein, several active site resi ...201626976751
recombinant thiopeptides containing noncanonical amino acids.thiopeptides are a subclass of ribosomally synthesized and posttranslationally modified peptides (ripps) with complex molecular architectures and an array of biological activities, including potent antimicrobial activity. here we report the generation of thiopeptides containing noncanonical amino acids (ncaas) by introducing orthogonal amber suppressor aminoacyl-trna synthetase/trna pairs into a thiocillin producer strain of bacillus cereus .we demonstrate that thiopeptide variants containing nc ...201626976568
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