Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| vascular complications and surgical interventions after world's largest q fever outbreak. | since chronic q fever often develops insidiously, and symptoms are not always recognized at an early stage, complications are often present at the time of diagnosis. we describe complications associated with vascular chronic q fever as found in the largest cohort of chronic q fever patients so far. | 2015 | 26365665 |
| genetic variation in tlr10 is not associated with chronic q fever, despite the inhibitory effect of tlr10 on coxiella burnetii-induced cytokines in vitro. | coxiella burnetii, the causative agent of q fever, is recognized by tlr2. tlr10 can act as an inhibitory receptor on tlr2-derived immune responses. therefore, we investigated the role of tlr10 on c. burnetii-induced cytokine production and assessed whether genetic polymorphisms in tlr10 influences the development of chronic q fever. hek293 cells, transfected with tlr2, tlr10 or tlr2/tlr10, and human peripheral blood mononuclear cells (pbmcs) in the presence of anti-tlr10, were stimulated with c. ... | 2016 | 26364993 |
| diseases transmitted by man's best friend: the dog. | the relationship between dogs and humans is ancient and mutually beneficial. dogs have served people well as companions, workmates, guides, and protectors. however, on occasion, dogs may injure humans through biting or may transmit pathogens resulting in a large number of problems ranging from a trivial rash to life-threatening bacteremia. given that there are more than 80 million pet dogs in the united states, it is worth knowing the potential problems that can result from canine exposure. annu ... | 2015 | 26350317 |
| rickettsia species in fleas collected from small mammals in slovakia. | epidemiological and epizootiological studies of rickettsia felis and other rickettsia spp. are very important, because their natural cycle has not yet been established completely. in total, 315 fleas (siphonaptera) of 11 species of ceratophyllidae, hystrichopsyllidae and leptopsyllidae families were tested for the presence of rickettsia species and coxiella burnetii with conventional and specific quantitative real-time pcr assays. fleas were collected from five rodent hosts (myodes glareolus, ap ... | 2015 | 26346455 |
| coxiella burnetii dna detected in domestic ruminants and wildlife from portugal. | coxiella burnetii is the etiological agent of q fever or coxiellosis, a zoonosis mainly affecting domestic ruminants. information on the population structure and epidemiology of c. burnetii in animals is scarce in portugal. evidence of c. burnetti infection was sought in domestic, wild and captive animals based on the detection of bacterial dna. tissue samples from 152 domestic animals (cattle=24, goats=51, sheep=76 and swine=1), 55 wild carnivores (egyptian mongoose=45, red fox=4, common genet= ... | 2015 | 26345258 |
| pulmonary inflammatory pseudotumor due to coxiella burnetii. case report and literature review. | a 58-year-old man was admitted because of respiratory failure, episodic fever with chilling, cough, malaise, fatigue, myalgia and weight loss lasting for at least one month. chest x-rays and ct scan of the chest showed bilateral pulmonary consolidations in upper lobes, the left lower lobe, and mediastinal lymphadenopathy. bronchoscopy with cytology was unremarkable. a needle ct-guided lung biopsy documented an inflammatory pseudotumor, lymphoplasmacytic type. serology showed high titer antibodie ... | 2017 | 26342254 |
| impact of is1111 insertion on the mlva genotyping of coxiella burnetii. | q fever epidemiological investigations of the likely sources of contamination may involve coxiella burnetii mlva for direct and rapid typing from clinical samples. however, little information is available with regards to pcr amplification failures in c. burnetii mlva typing. this paper focuses on difficulties encountered with mlva loci that may impact the interpretation of mlva data and shows that some loci may constitute hotspots for mutational events. mlva genotyping, using 17 different loci, ... | 2016 | 26342253 |
| integrating interdisciplinary methodologies for one health: goat farm re-implicated as the probable source of an urban q fever outbreak, the netherlands, 2009. | in spring 2008, a goat farm experiencing q fever abortions ("farm a") was identified as the probable source of a human q fever outbreak in a dutch town. in 2009, a larger outbreak with 347 cases occurred in the town, despite no clinical q fever being reported from any local farm. | 2015 | 26336097 |
| an inventory of concerns behind blood safety policies in five western countries. | the availability of costly safety measures against transfusion-transmissible infections forces western countries to confront difficult ethical questions. how to decide about implementing such measures? when are such decisions justified? as a preliminary to addressing these questions, we assessed which concerns shape actual donor blood safety policymaking in five western countries. | 2015 | 26331441 |
| biogenesis of the lysosome-derived vacuole containing coxiella burnetii. | coxiella burnetii utilizes a type iv secretion system (t4ss) to modify host endomembrane transport systems to form a unique lysosome-derived niche called the coxiella-containing vacuole (ccv). although the ccv has lysosomal properties, this organelle displays distinct characteristics such as homotypic fusion and a cholesterol enriched limiting membrane, in addition to robustly interacting with autophagosomes. this review describes recent advances in understanding ccv biogenesis and the mechanism ... | 2017 | 26327296 |
| coxiella burnetii infections in sheep or goats: an opinionated review. | q fever is an almost ubiquitous zoonosis caused by coxiella burnetii, which is able to infect several animal species, as well as humans. cattle, sheep and goats are the primary animal reservoirs. in small ruminants, infections are mostly without clinical symptoms, however, abortions and stillbirths can occur, mainly during late pregnancy. shedding of c. burnetii occurs in feces, milk and, mostly, in placental membranes and birth fluids. during parturition of infected small ruminants, bacteria fr ... | 2015 | 26315774 |
| whole genome pcr scanning (wgps) of coxiella burnetii strains from ruminants. | coxiella burnetii is the causative agent of q fever, a zoonosis that spreads from ruminants to humans via the inhalation of aerosols contaminated by livestock's birth products. this study aimed to compare the genomes of strains isolated from ruminants by "whole genome pcr scanning (wgps)" in order to identify genomic differences. c. burnetii isolated from different ruminant hosts were compared to the nine mile reference strain using wgps. the identified genomic regions of differences (rds) were ... | 2016 | 26315064 |
| coxiella burnetii and leishmania mexicana residing within similar parasitophorous vacuoles elicit disparate host responses. | coxiella burnetii is a bacterium that thrives in an acidic parasitophorous vacuole (pv) derived from lysosomes. leishmania mexicana, a eukaryote, has also independently evolved to live in a morphologically similar pv. as coxiella and leishmania are highly divergent organisms that cause different diseases, we reasoned that their respective infections would likely elicit distinct host responses despite producing phenotypically similar parasite-containing vacuoles. the objective of this study was t ... | 2015 | 26300862 |
| doxycycline assay hair samples for testing long-term compliance treatment. | many patients undergoing long-term doxycycline treatment do not regularly take their treatment because of photosensitivity. our objective was to create an assay for determining doxycycline levels and to use hair samples for monitoring the compliance over a longer period of time. | 2015 | 26299894 |
| [detection of coxiella burnetii in dairy cattle bulk tank milk and single tank milk samples by confirmatory testing]. | q fever is a worldwide zoonotic disease caused by the pathogen coxiella (c.) burnetii. a wide range of animal species is susceptible to this intracellular bacterium with great importance in ruminants. human infections occur mainly by airborne transmission. c burnetii was detected in animal products such as raw milk, raw-milk cheese and butter prepared from raw milk as well as in the meat of infected animals. in cattle milk, the pathogen was detected up to 13 months after calving. the risk of hum ... | 2015 | 26281438 |
| chronic q fever diagnosis— consensus guideline versus expert opinion | chronic q fever, caused by coxiella burnetii, has high mortality and morbidity rates if left untreated. controversy about the diagnosis of this complex disease has emerged recently. we applied the guideline from the dutch q fever consensus group and a set of diagnostic criteria proposed by didier raoult to all 284 chronic q fever patients included in the dutch national chronic q fever database during 2006–2012. of the patients who had proven cases of chronic q fever by the dutch guideline, 46 (3 ... | 2015 | 26277798 |
| zoonotic risks from small ruminants. | zoonoses are infections that spread naturally between species (sometimes by a vector) from animals to other animal species or to humans or from humans to animals. most of the zoonoses diagnosed in sheep and goats are transmitted by close contact of man with these animals and are, more often, occupational diseases that principally affect breeders, veterinarians and/or slaughterhouse workers. some other diseases have an airborne transmission and affect the population in the vicinity of sheep/goat ... | 2015 | 26275853 |
| coxiella burnetii (q fever) infection in farmed ruminants in three northeastern provinces and inner mongolia autonomous region, china. | coxiella burnetii is the pathogenic agent of q fever, a zoonosis that occurs worldwide. in china, the organism infects people and a wide range of wild and farmed animals. however, little is known about c. burnetii infection in farmed ruminants in northeastern china, and there appears to have been no previous study on sika deer. the present study was conducted to determine the seroprevalence of c. burnetii in farmed ruminants in this region of china. a total of 3147 domestic ruminants (784 sheep, ... | 2015 | 26273813 |
| a comparison of patients with q fever fatigue syndrome and patients with chronic fatigue syndrome with a focus on inflammatory markers and possible fatigue perpetuating cognitions and behaviour. | comparison of q fever fatigue syndrome (qfs) and chronic fatigue syndrome (cfs) patients, with a focus on markers of inflammation and fatigue-related cognitive-behavioural variables. | 2015 | 26272528 |
| the is1111 insertion sequence used for detection of coxiella burnetii is widespread in coxiella-like endosymbionts of ticks. | coxiella is a genus of obligate intracellular bacteria engaged in a variety of interactions with eukaryotes. the type species, coxiella burnetii, infects several vertebrate species, including humans, and is the causative agent of q fever. multiple copies of a specific transposable element, the insertion sequence is1111, are present in the genome of c. burnetii and are routinely used for confirmation of q fever cases. recently, many coxiella-like bacteria that are closely related but genetically ... | 2015 | 26269380 |
| [q fever]. | the article summarizes some important recently identified findings about the coxiella burnetii disease, q fever. beside new diagnostic parameters for follow-up issues, the importance of a timely identification of chronic q fever and the peculiarities of the post q fever fatigue syndrome are depicted. | 2015 | 26261927 |
| circulation of coxiella burnetii in a naturally infected flock of dairy sheep: shedding dynamics, environmental contamination, and genotype diversity. | q fever is a worldwide zoonosis caused by coxiella burnetii. domestic ruminants are considered to be the main reservoir. sheep, in particular, may frequently cause outbreaks in humans. because within-flock circulation data are essential to implementing optimal management strategies, we performed a follow-up study of a naturally infected flock of dairy sheep. we aimed to (i) describe c. burnetii shedding dynamics by sampling vaginal mucus, feces, and milk, (ii) assess circulating strain diversity ... | 2015 | 26253679 |
| coxiella burnetii infection is lower in children than in adults after community exposure: overlooked cause of infrequent q fever reporting in the young. | q fever is rarely reported in children/adolescents. although lower reporting rates are commonly attributed to milder disease and subsequent underdiagnosis in infected children/adolescents, pertinent evidence is scarce. we present data from a large, well-defined single-point source outbreak of q fever to fill this gap. | 2015 | 26252570 |
| lamp proteins account for the maturation delay during the establishment of the coxiella burnetii-containing vacuole. | the obligate intracellular pathogen coxiella burnetii replicates in a large phagolysosomal-like vacuole. currently, both host and bacterial factors required for creating this replicative parasitophorous c. burnetii-containing vacuole (pv) are poorly defined. here, we assessed the contributions of the most abundant proteins of the lysosomal membrane, lamp-1 and lamp-2, to the establishment and maintenance of the pv. whereas these proteins were not critical for uptake of c. burnetii, they influenc ... | 2016 | 26249821 |
| estimated herd prevalence and sequence types of coxiella burnetii in bulk tank milk samples from commercial dairies in indiana. | coxiella burnetii is the etiologic agent of q fever, a zoonotic disease causing influenza-like illness, pregnancy loss, cardiovascular disease and chronic fatigue syndrome in people. c. burnetii is considered to be enzootic in ruminants, but clinical signs of infection do not always manifest. national studies have documented the presence of c. burnetii in dairy herds in indiana. this represents an opportunity to better characterize the distribution and prevalence of c. burnetii infection at the ... | 2015 | 26248712 |
| serological survey of antibodies to toxoplasma gondii and coxiella burnetii in rodents in north-western african islands (canary islands and cape verde). | coxiella burnetii and toxoplasma gondii are intracellular parasites that cause important reproductive disorders in animals and humans worldwide, resulting in high economic losses. the aim of the present study was to analyse the possible role of peridomestic small mammals in the maintenance and transmission of c. burnetii and t. gondii in the north-western african archipelagos of the canary islands and cape verde, where these species are commonly found affecting humans and farm animals. between 2 ... | 2015 | 26244685 |
| infective endocarditis in the military patient. | infective endocarditis (ie) is a potentially fatal cardiac infection associated with an inhospital mortality rate of up to 22%. fifty per cent of ie cases develop in patients with no known history of valve disease. it is therefore important to remain vigilant to the possibility of the diagnosis in patients with a febrile illness and unknown source. from a military perspective, our patients are unique due to the breadth of pathogens they are exposed to, and blood-culture-negative ie is a risk. in ... | 2015 | 26243804 |
| serum level of c-reactive protein is not a parameter to determine the difference between viral and atypical bacterial infections. | c-reactive protein (crp) is an acute-phase reactant that increases in the circulation in response to a variety of inflammatory stimuli. elevated levels in serum during several infectious diseases have been reported. in this study, a highly sensitive crp enzyme immunoassay was used to evaluate serum crp values in patients with viral and atypical bacterial infections. patients (n = 139) with different viral or atypical bacterial infections (systemic or respiratory) and healthy controls (n = 40) we ... | 2016 | 26241406 |
| a creative approach to mycotic abdominal aortic aneurysm secondary to coxiella burnetii infection. | 2015 | 26234001 | |
| coxiella burnetii seropositivity and associated risk factors in goats in ontario, canada. | coxiella burnetii is a zoonotic bacterium, and infection in goats with this bacterium can result in abortion, stillbirth or birth of non-viable kids. a cross-sectional study was conducted to identify the seroprevalence and risk factors for c. burnetii exposure in ontario goats. sera were collected between august 2010 and february 2012, and tested for c. burnetii specific antibodies using an enzyme-linked immunosorbent assay (idexx). overall, 63.2% (48/76, 95% ci=51.9-73.4) of farms had one or mo ... | 2015 | 26231909 |
| industrial food animal production and community health. | industrial food animal production (ifap) is a source of environmental microbial and chemical hazards. a growing body of literature suggests that populations living near these operations and manure-applied crop fields are at elevated risk for several health outcomes. we reviewed the literature published since 2000 and identified four health outcomes consistently and positively associated with living near ifap: respiratory outcomes, methicillin-resistant staphylococcus aureus (mrsa), q fever, and ... | 2015 | 26231503 |
| molecular detection of zoonotic tick-borne pathogens from ticks collected from ruminants in four south african provinces. | ticks carry and transmit a remarkable array of pathogens including bacteria, protozoa and viruses, which may be of veterinary and/or of medical significance. with little to no information regarding the presence of tick-borne zoonotic pathogens or their known vectors in southern africa, the aim of our study was to screen for anaplasma phagocytophilum, borrelia burgdorferi, coxiella burnetii, rickettsia species and ehrlichia ruminantium in ticks collected and identified from ruminants in the easte ... | 2015 | 26227797 |
| q fever chronic osteomyelitis in two children. | we report 2 cases of chronic q fever osteomyelitis in 10- and 5-year-old girls who presented with distal right femoral and left parasternal granulomatous osteomyelitis, respectively. both were treated with ciprofloxacin and rifampin with good response. q fever osteomyelitis is a challenging diagnosis in children, and the choice of antimicrobial treatment is difficult because of limited available data. | 2015 | 26226441 |
| seroprevalence and occupational risk survey for coxiella burnetii among exposed workers in sicily, southern italy. | the aim of this survey was to assess the seroprevalence of antibodies against coxiella burnetii (c. burnetii) in subjects at risk of exposure in sicily, southern italy. | 2015 | 26224501 |
| vaccination schedules in small ruminant farms. | development and implementation of health management plans is the cornerstone of profitable farms; prevention of microbial diseases by means of vaccination is an integral part of such a plan. in every production type and management system in small ruminants, microbial diseases have a major significance, hence their proper control must be based in good health management practices, including use of effective and safe vaccines. development of various types of vaccines is evolving very quickly in rec ... | 2015 | 26220514 |
| evaluation of patients with community-acquired pneumonia caused by zoonotic pathogens in an area with a high density of animal farms. | intensive animal farming could potentially lead to outbreaks of infectious diseases. clinicians are at the forefront of detecting unusual diseases, but the lack of specificity of zoonotic disease symptoms makes this a challenging task. we evaluated patients with community-acquired pneumonia (cap) with known and unknown aetiology in an area with a high livestock density and a potential association with animal farms in the proximity. between 2008 and 2009, a period coinciding with a large q fever ... | 2016 | 26214299 |
| bartonella spp. and coxiella burnetii associated with community-acquired, culture-negative endocarditis, brazil. | we evaluated culture-negative, community-acquired endocarditis by using indirect immunofluorescent assays and molecular analyses for bartonella spp. and coxiella burnetii and found a prevalence of 19.6% and 7.8%, respectively. our findings reinforce the need to study these organisms in patients with culture-negative, community-acquired endocarditis, especially b. henselae in cat owners. | 2015 | 26197233 |
| differentiation of acute q fever from other infections in patients presenting to hospitals, the netherlands(1). | differentiating acute q fever from infections caused by other pathogens is essential. we conducted a retrospective case-control study to evaluate differences in clinical signs, symptoms, and outcomes for 82 patients with acute q fever and 52 control patients who had pneumonia, fever and lower respiratory tract symptoms, or fever and hepatitis, but had negative serologic results for q fever. patients with acute q fever were younger and had higher c-reactive protein levels but lower leukocyte coun ... | 2015 | 26196955 |
| first report of coxiella burnetii seroprevalence in tibetan sheep in china. | query (q) fever is a vector-borne zoonosis caused by the obligate intracellular pathogen coxiella burnetii. animals, including dogs, cats, cattle, and sheep, can be infected by c. burnetii. however, little information is available about c. burnetii infection in tibetan sheep in china. antibodies against c. burnetii in tibetan sheep serums, which were collected from maqu county, tianzhu county and nyingchi prefecture, china, between 2011 and 2013, were examined by enzyme-linked immunosorbent assa ... | 2015 | 26186513 |
| applying an inducible expression system to study interference of bacterial virulence factors with intracellular signaling. | the technique presented here allows one to analyze at which step a target protein, or alternatively a small molecule, interacts with the components of a signaling pathway. the method is based, on the one hand, on the inducible expression of a specific protein to initiate a signaling event at a defined and predetermined step in the selected signaling cascade. concomitant expression, on the other hand, of the gene of interest then allows the investigator to evaluate if the activity of the expresse ... | 2015 | 26168006 |
| [brucellosis as a cause of hemophagocytic syndrome]. | hemophagocytic lymphohistiocytosis (hlh) is a rare syndrome of excessive inflammation and tissue destruction due to abnormal immune activation and inflammation. hlh can occur primarily due to genetic etiology, or secondarily associated with malignancies, autoimmmune diseases or infections. there are a number of reports that revealed the relationship of hemophagocytosis with brucellosis. in this report, we described a brucellosis-related hlh case. a 73-year-old male who work as farmer was admitte ... | 2015 | 26167830 |
| economic aspects of q fever control in dairy goats. | this paper presents an economic analysis of q fever control strategies in dairy goat herds in the netherlands. evaluated control strategies involved vaccination strategies (being either preventive or reactive) and reactive non-vaccination strategies (i.e., culling or breeding prohibition). reactive strategies were initiated after pcr positive bulk tank milk or after an abortion storm (abortion percentage in the herd of 5% or more). preventive vaccination eradicates q fever in a herd on average w ... | 2015 | 26164531 |
| long-term serological follow-up of acute q-fever patients after a large epidemic. | serological follow-up of acute q-fever patients is important for detection of chronic infection but there is no consensus on its frequency and duration. the 2007-2009 q-fever epidemic in the netherlands allowed for long-term follow-up of a large cohort of acute q-fever patients. the aim of this study was to validate the current follow-up strategy targeted to identify patients with chronic q-fever. | 2015 | 26161658 |
| host and environmental factors modulate the exposure of free-ranging and farmed red deer (cervus elaphus) to coxiella burnetii. | the control of multihost pathogens, such as coxiella burnetii, should rely on accurate information about the roles played by the main hosts. we aimed to determine the involvement of the red deer (cervus elaphus) in the ecology of c. burnetii. we predicted that red deer populations from broad geographic areas within a european context would be exposed to c. burnetii, and therefore, we hypothesized that a series of factors would modulate the exposure of red deer to c. burnetii. to test this hypoth ... | 2015 | 26150466 |
| quantitation of viable coxiella burnetii in milk using an integrated cell culture-polymerase chain reaction (icc-pcr) assay. | the obligate intracellular pathogen coxiella burnetii has long been considered the most heat resistant pathogen in raw milk, making it the reference pathogen for determining pasteurisation conditions for milk products. new milk formulations and novel non-thermal processes require validation of effectiveness which requires a more practical method for analysis than using the currently used animal model for assessing coxiella survival. also, there is an interest in better characterising thermal ina ... | 2015 | 26143937 |
| delayed diagnosis of q fever: finally diagnosed after elective cardiac surgery. | q fever is a bacterial infection caused by coxiella burnetti. it can cause both acute and chronic illness. chronic qf can present as a variety of clinical syndromes. a common and critical manifestation is endocarditis which can present atypically and is easily missed. this case describes a man who, after extensive investigation for splenomegaly and pancytopenia by several specialties, was finally diagnosed with q fever endocarditis after unexpected aortic valve abnormalities found during electiv ... | 2015 | 26140783 |
| detection of lyme disease and q fever agents in wild rodents in central italy. | the maintenance of tick-borne disease agents in the environment strictly depends on the relationship between tick vectors and their hosts, which act as reservoirs for these pathogens. a pilot study aimed to investigate wild rodents as reservoirs for zoonotic tick-borne pathogens (borrelia burgdorferi sensu lato (s.l.), coxiella burnetii, francisella tularensis, and anaplasma phagocytophilum) was carried out in an area of gran sasso e monti della laga national park (abruzzi region, central italy) ... | 2015 | 26134933 |
| population screening for chronic q-fever seven years after a major outbreak. | from 2007 through 2010, the netherlands experienced a large q-fever epidemic, with 4,107 notifications. the most serious complication of q-fever is chronic q-fever. | 2015 | 26132155 |
| reorganization of actin cytoskeleton in l929 cells infected with coxiella burnetii strains isolated from placenta and foetal brain of sheep (sardinia, italy). | coxiella burnetii, the etiological agent of q fever, is a zoonotic pathogen distributed worldwide. it has been reported that virulent strains of c. burnetii are poorly internalized by monocytes compared to avirulent variants. virulence is also associated to the formation of pseudopodal extensions and transient reorganization of filamentous actin. in this article, we investigated the ability of 2 coxiella strains isolated from ovine aborted samples to induce reorganization of the actin cytoskelet ... | 2015 | 26129661 |
| q fever. | q fever is a zoonotic disease found throughout the world. it is caused by the intracellular gram-negative bacterium coxiella burnetii. infection by c. burnetii occurs primarily by inhalation of the aerosolized bacteria from birthing animals or contaminated dust. the bacterium is very resistant to drying and heat, and is considered highly endemic in the middle east, where it is likely underdiagnosed. special operations forces medical providers should be aware of this disease, which must be in the ... | 2015 | 26125173 |
| coxiella burnetii infection in a patient from a rural area of monteria, colombia. | q fever is a zoonosis caused by coxiella burnetii. in colombia, there have been very few human cases reported to date. this report describes the case of a 56-year-old patient with a background in agriculture and livestock handling. an indirect immunofluorescence assay (ifa) showed high titers of igg for c. burnetii anti-phase i (1: 256) and anti-phase ii (1:1024). for the next six months the patient's igg antibody titers remained high, and after treatment with doxycycline, the igg antibody titer ... | 2015 | 26120864 |
| dhr-icmr guidelines for diagnosis & management of rickettsial diseases in india. | rickettsial diseases, caused by a variety of obligate intracellular, gram-negative bacteria from the genera rickettsia, orientia, ehrlichia, neorickettsia, neoehrlichia, and anaplasma, belonging to the alphaproteobacteria, are considered some of the most covert emerging and re-emerging diseases and are being increasingly recognized. among the major groups of rickettsioses, commonly reported diseases in india are scrub typhus, murine flea-borne typhus, indian tick typhus and q fever. rickettsial ... | 2015 | 26112842 |
| [prolonged disease in a farmer with simultaneous q fever pneumonia and subacute hypersensitivity pneumonitis]. | this case report describes the first incident of a patient with simultaneous occurrence of q fever pneumonia and subacute hypersensitivity pneumonitis (hp). the 52-year-old patient was from his occupation as a farmer exposed to agents causing both diseases. except for a weight loss in subacute/chronic incidents of hp the symptoms of q fever and hp are similar. however, differences in ct findings and histopathology make it possible to differentiate between the two diseases. this report highlights ... | 2015 | 26099187 |
| seroepidemiology of q fever in one-humped camel population in northeast iran. | coxiella burnetii, an obligate intracellular bacterium, is the causative agent of important zoonotic q fever. it is the etiological agent of coxiellosis or q fever in animals and human. this seroepidemiological survey was conducted to determine the seroprevalence of coxiellosis in increasingly camel raised population in vast area of khorasan (north, south, and razavi) provinces, northeast iran. using cluster random sampling strategy, 167 camels in 11 counties were selected as serum samples. sera ... | 2015 | 26070292 |
| effects of long-term vaccination against coxiella burnetii on the fertility of high-producing dairy cows. | the impact of long-term vaccination against coxiella burnetii on the fertility of cows was studied. double vaccinations three weeks apart at the start of the third trimester of gestation in each of two consecutive pregnancies were applied. the final study population consisted of 410 cows after the first vaccination round. based on the odds ratios, the likelihood of early fetal loss (pregnancy loss following a positive pregnancy diagnosis before day 90 of gestation) was higher in control cows (or ... | 2015 | 26051261 |
| [non-hiv infectious disease outpatient consultations: a 5-year study in a swiss university hospital]. | few studies have examined the workload or clinical spectrum of non-hiv infectious diseases outpatient consultations (idoc). this retrospective study aims to describe idoc referrals over the past 5 years. in total, 483 patients were referred (with an increase of 63% between 2009 and 2013). most referrals were received from primary care clinicians (45%). median patient age was 47 years, 57% of patients were men and 17% were immunosuppressed. of the diagnoses retained, 74% were infectious, 20% were ... | 2015 | 26050301 |
| [differential diagnostic and therapeutic aspects of q-fever in a male prisoner. case report]. | the authors describe the case of a 46-year-old man, who developed atypical pneumonia caused by coxiella burnetii. chest x-ray revealed interstitial pneumonia. western blot and elisa test were positive for coxiella burnetii antibody. after treatment with doxycyclin and amoxicillin supplemented with vitamin b6 for 10 days, the patient displayed a clinical improvement. the authors conclude that in cases with atypical pneumonia, coxiella burnetii antibody as well as other bacterial or viral antibodi ... | 2015 | 26042781 |
| chronic q fever in a renal transplant recipient: a case report. | q fever is a zoonosis caused by coxiella burnetii that presents with a wide spectrum of acute and chronic manifestations. progression to chronic q fever is frequently associated with valve and vascular prosthesis, aneurisms, pregnancy, immunosuppression, and advanced chronic kidney disease. we present a case of a kidney transplant recipient with persistent fever of unknown origin, negative blood cultures, anemia, and increased erythrocyte sedimentation rate (esr) and c-reactive protein (crp). q ... | 2015 | 26036515 |
| structural genomics for drug design against the pathogen coxiella burnetii. | coxiella burnetii is a highly infectious bacterium and potential agent of bioterrorism. however, it has not been studied as extensively as other biological agents, and very few of its proteins have been structurally characterized. to address this situation, we undertook a study of critical metabolic enzymes in c. burnetii that have great potential as drug targets. we used high-throughput techniques to produce novel crystal structures of 48 of these proteins. we selected one protein, c. burnetii ... | 2015 | 26033498 |
| neutrophils play an important role in protective immunity against coxiella burnetii infection. | coxiella burnetii is an obligate intracellular gram-negative bacterium that causes the zoonotic disease q fever. although q fever is mainly transmitted by aerosol infection, study of the immune responses in the lung following pulmonary c. burnetii infection is lacking. neutrophils are considered the first immune cell to migrate into the lung and play an important role in host defense against aerosol infection with microbial pathogens. however, the role of neutrophils in the host defense against ... | 2015 | 26015476 |
| generation and multi-phenotypic high-content screening of coxiella burnetii transposon mutants. | invasion and colonization of host cells by bacterial pathogens depend on the activity of a large number of prokaryotic proteins, defined as virulence factors, which can subvert and manipulate key host functions. the study of host/pathogen interactions is therefore extremely important to understand bacterial infections and develop alternative strategies to counter infectious diseases. this approach however, requires the development of new high-throughput assays for the unbiased, automated identif ... | 2015 | 25992686 |
| presumptive q fever myocarditis associated with coxiella burnetii infection of a homograft valve in the outflow tract of the right ventricle: review and case report. | a man with pulmonary atresia and a subaortic ventricular septal defect had a radical surgical repair at the age of 16 years with correction of pulmonary atresia by replacement with an aortic valve homograft. this later became infected with coxiella burnetii. he died at the age of 27 years from renal failure caused by glomerulonephritis and was found to have a most unusual form of widespread focal myocardial degeneration with dystrophic calcification. the diagnosis of q fever was confirmed by pos ... | 2015 | 25990851 |
| serological survey using elisa to determine the prevalence of coxiella burnetii infection (q fever) in sheep and goats in great britain. | a survey of coxiella burnetii infection (q fever) in sheep flocks and goat herds in great britain was undertaken. a total of 5791 sheep (384 flocks) and 522 goats (145 herds) were examined for c. burnetii antibodies using an elisa. overall, 53 sheep (37 flocks), and four goats (four herds), tested positive. estimates of individual animal, between-flock/-herd and within-flock/-herd crude prevalences were 0·9%, 10·2% and 9·0%, respectively, for sheep, and 0·8%, 3% and 26·3%, respectively, for goat ... | 2016 | 25990008 |
| european rabbits as reservoir for coxiella burnetii. | we studied the role of european rabbits (oryctolagus cuniculus) as a reservoir for coxiella burnetii in the iberian region. high individual and population seroprevalences observed in wild and farmed rabbits, evidence of systemic infections, and vaginal shedding support the reservoir role of the european rabbit for c. burnetii. | 2015 | 25988670 |
| polymicrobial q fever and enterococcal aortic prosthetic valve endocarditis with aortic root abscess. | polymicrobial endocarditis is uncommon. we present a case of polymicrobial endocarditis caused by enterococcus faecalis and coxiella burnetii and review previous cases of polymicrobial endocarditis involving coxiella burnetii. testing for q fever should be considered in any patient with endocarditis living in an endemic area. | 2015 | 25988443 |
| underrecognized arthropod-borne and zoonotic pathogens in northern and northwestern thailand: serological evidence and opportunities for awareness. | although scrub typhus and murine typhus are well-described tropical rickettsial illnesses, especially in southeast asia, only limited evidence is available for rickettsia-like pathogens contributing to the burden of undifferentiated febrile illness. using commercially available kits, this study measured immunoglobulin g (igg) antibody seroprevalence for coxiella burnetii, ehrlichia chaffeensis, bartonella henselae, anaplasma phagocytophilum, and spotted fever group rickettsiae (sfgr) in 375 pati ... | 2015 | 25988437 |
| loss of tss1 in hypervirulent coxiella burnetii 175, the causative agent of q fever in french guiana. | in french guiana, the unique coxiella burnetii circulating genotype 17 causes 24% of community-acquired pneumonia, the highest prevalence ever described. to explain this unusual virulence, we performed a comparative genomic analysis of strain cb175, which was isolated from a patient from french guiana. cb175 has a greater number of mutations in genes involved in metabolism compared with the nine mile i strain. we found a 6105bp fragment missing in cb175, which corresponds to the type 1 secretion ... | 2015 | 25982513 |
| exposure to low doses of coxiella burnetii caused high illness attack rates: insights from combining human challenge and outbreak data. | as a major zoonotic pathogen, characterization of the infectivity and pathogenicity of coxiella burnetii is essential to understand q-fever epidemiology. | 2015 | 25979276 |
| the recent evolution of a maternally-inherited endosymbiont of ticks led to the emergence of the q fever pathogen, coxiella burnetii. | q fever is a highly infectious disease with a worldwide distribution. its causative agent, the intracellular bacterium coxiella burnetii, infects a variety of vertebrate species, including humans. its evolutionary origin remains almost entirely unknown and uncertainty persists regarding the identity and lifestyle of its ancestors. a few tick species were recently found to harbor maternally-inherited coxiella-like organisms engaged in symbiotic interactions, but their relationships to the q fever ... | 2015 | 25978383 |
| cultural drivers and health-seeking behaviours that impact on the transmission of pig-associated zoonoses in lao people's democratic republic. | pig rearing is an important income source in the lao people's democratic republic (pdr), with many smallholder farmers using traditional free-range pig production systems. despite the potentially significant health risks posed by pig production regarding pig-associated zoonoses, information on the sociocultural drivers of these zoonoses is significantly lacking. this review summarises the existing sociocultural knowledge on eight pig-associated zoonoses suspected to be endemic in southeast asia: ... | 2015 | 25973203 |
| cloning, expression, and characterization of a coxiella burnetii cu/zn superoxide dismutase. | periplasmically localized copper-zinc co-factored superoxide dismutase (sodc) enzymes have been identified in a wide range of gram-negative bacteria and are proposed to protect bacteria from exogenously produced toxic oxygen radicals, which indicates the potential significance of a coxiella burnetii sodc. | 2015 | 25962997 |
| coxiella burnetii exposure in northern sea otters enhydra lutris kenyoni. | valvular endocarditis has been well described in northern sea otters enhydra lutris kenyoni of alaska and in many cases no cause has been identified. it is also one of the most common conditions observed in people with chronic coxiella burnetii infection. given the high levels of c. burnetii exposure in marine mammals distributed throughout the same geographic range as the northern sea otter, and the presence of valvular lesions seen in otters, the objective of this study was to determine the le ... | 2015 | 25958809 |
| first draft genome sequence of a human coxiella burnetii isolate, originating from the largest q fever outbreak ever reported, the netherlands, 2007 to 2010. | in 2009, coxiella burnetii caused a large regional outbreak of q fever in south limburg, the netherlands. here, we announce the genome draft sequence of a human c. burnetii isolate, strain nl-limburg, originating from this outbreak, including a brief summary of the genome's general features. | 2015 | 25953164 |
| evaluation of associations among coxiella burnetii and reproductive abnormalities in cats. | coxiella burnetii is an obligate intracellular bacterium that is found worldwide, is associated or suggested to be associated with reproductive abnormalities in a number of species including cats, and is the cause of q fever in humans. in a previous study, c burnetii dna was amplified from the uterine tissues of 8.5% of client-owned cats in the usa but reproductive history was unknown and histopathological examination was not performed. in this study, uterine tissues of 26 normal cats and 11 cat ... | 2016 | 25944579 |
| dysregulation of serum gamma interferon levels in vascular chronic q fever patients provides insights into disease pathogenesis. | a large community outbreak of q fever occurred in the netherlands in the period 2007 to 2010. some of the infected patients developed chronic q fever, which typically includes pathogen dissemination to predisposed cardiovascular sites, with potentially fatal consequences. to identify the immune mechanisms responsible for ineffective clearance of coxiella burnetii in patients who developed chronic q fever, we compared serum concentrations of 47 inflammation-associated markers among patients with ... | 2015 | 25924761 |
| coxiella burnetii infections in small ruminants and humans in switzerland. | the recent q fever epidemic in the netherlands raised concerns about the potential risk of outbreaks in other european countries. in switzerland, the prevalence of q fever in animals and humans has not been studied in recent years. in this study, we describe the current situation with respect to coxiella (c.) burnetii infections in small ruminants and humans in switzerland, as a basis for future epidemiological investigations and public health risk assessments. specific objectives of this cross- ... | 2017 | 25922932 |
| recent advances in the study of q fever epidemiology, diagnosis and management. | q fever is a worldwide zoonotic infection with an epidemiological pattern consisting of sporadic cases, endemic situations and outbreaks of unsuspected magnitude, as occurred in holland. this event highlighted the fact that the term "chronic q fever" is misleading and should be avoided. here, we review recent advances in the understanding and management of this disease. there have been clonal outbreaks of confirmed "geotypes," such as the outbreaks in french guiana, where a very high incidence w ... | 2015 | 25917809 |
| coverage of the 2011 q fever vaccination campaign in the netherlands, using retrospective population-based prevalence estimation of cardiovascular risk-conditions for chronic q fever. | in 2011, a unique q fever vaccination campaign targeted people at risk for chronic q fever in the southeast of the netherlands. general practitioners referred patients with defined cardiovascular risk-conditions (age >15 years). prevalence rates of those risk-conditions were lacking, standing in the way of adequate planning and coverage estimation. we aimed to obtain prevalence rates retrospectively in order to estimate coverage of the q fever vaccination campaign. | 2015 | 25909712 |
| chloroform-methanol residue of coxiella burnetii markedly potentiated the specific immunoprotection elicited by a recombinant protein fragment rompb-4 derived from outer membrane protein b of rickettsia rickettsii in c3h/hen mice. | the obligate intracellular bacteria, rickettsia rickettsii and coxiella burnetii, are the potential agents of bio-warfare/bio-terrorism. here c3h/hen mice were immunized with a recombinant protein fragment romp-4 derived from outer membrane protein b, a major protective antigen of r. rickettsii, combined with chloroform-methanol residue (cmr) extracted from phase i c. burnetii organisms, a safer q fever vaccine. these immunized mice had significantly higher levels of igg1 and igg2a to rompb-4 an ... | 2015 | 25909586 |
| a survey of zoonotic pathogens carried by non-indigenous rodents at the interface of the wet tropics of north queensland, australia. | in 1964, brucella was isolated from rodents trapped in wooroonooran national park (wnp), in northern queensland, australia. genotyping of bacterial isolates in 2008 determined that they were a novel brucella species. this study attempted to reisolate this species of brucella from rodents living in the boundary area adjacent to wnp and to establish which endo- and ecto-parasites and bacterial agents were being carried by non-indigenous rodents at this interface. seventy non-indigenous rodents wer ... | 2017 | 25906923 |
| an unusual manifestation of q fever: peritonitis. | q fever has rarely been reported and can be difficult to diagnose, especially in immunocompromised patients. in the present report, we describe an unusual case of q fever that presented as peritonitis and was treated with long-term combination therapy with doxycycline, ciprofloxacin and rifampicin for five weeks in a patient who had been on peritoneal dialysis for six years due to hypertensive nephropathy. | 2016 | 25899561 |
| prevalence of coxiella burnetii infection in humans occupationally exposed to animals in poland. | coxiella burnetii is the etiological agent of q fever, and outbreaks of q fever have been reported in different parts of europe both in animals and humans. human infections are mostly associated with infections in ruminants, e.g., sheep, goats, and cows. various professional groups are occupationally exposed to infection with c. burnetii. the aim of this study was investigate the prevalence of c. burnetii in farm workers. serum samples were collected from 151 persons from six different regions o ... | 2015 | 25897813 |
| q fever and fever of unknown origin. are the chronic forms of these conditions preventable? | 2015 | 25896258 | |
| improved correlation of human q fever incidence to modelled c. burnetii concentrations by means of an atmospheric dispersion model. | atmospheric dispersion models (adms) may help to assess human exposure to airborne pathogens. however, there is as yet limited quantified evidence that modelled concentrations are indeed associated to observed human incidence. | 2015 | 25888858 |
| the aetiology of community associated pneumonia in children in nanjing, china and aetiological patterns associated with age and season. | viral and atypical bacterial pathogens play an important role in respiratory tract infection. using the pneumoslide igm test, the presented study explored the aetiology of community-acquired pneumonia and investigated further whether there was an association between age or season and aetiological organisms. | 2015 | 25879996 |
| first isolation of coxiella burnetii from clinical material by cell-free medium (accm2). | a disadvantage in q fever diagnostics and research is the insensitive and difficult culture of coxiella burnetii. this intracellular organism can only be isolated using embryonated eggs, animal hosts, or mammalian cell culture. in consequence, it has only been possible to isolate a few strains from human patients. here, we describe the first isolation of c. burnetii from a clinical specimen using the recently developed cell-free medium acidified citrate cysteine medium 2 (accm2). we screened the ... | 2015 | 25876216 |
| tick species (acari: ixodida) in antalya city, turkey: species diversity and seasonal activity. | ticks (acari: ixodida) are an important group of ectoparasites of vertebrates. most species are known vectors of diseases including lyme disease, q fever, and crimean-congo hemorrhagic fever. a 3-year research was conducted in antalya, turkey, to determine tick species composition, seasonal abundance, and spatial distribution. the study was carried out in five districts (aksu, dosemealtı, kepez, konyaaltı, and muratpasa) of antalya metropolitan municipality area in turkey, between may 2010 and m ... | 2015 | 25869959 |
| nationwide registry-based ecological analysis of q fever incidence and pregnancy outcome during an outbreak in the netherlands. | whether areas affected by q fever during a large outbreak (2008-2010) had higher rates of adverse pregnancy outcomes than areas not affected by q fever. | 2015 | 25862010 |
| molecular detection of coxiella burnetii using an alternative loop-mediated isothermal amplification assay (lamp). | q fever, caused by coxiella burnetii, is a worldwide zoonosis with important consequences for human and animal health. in livestock, the diagnosis, using direct and indirect techniques, is challenging even if to tackle coxiellosis in domesticated animals a rapid diagnosis is crucial. in the recent years, new molecular methods have been developed to overcome these issues. several polymerase chain reaction (pcr) assays have been studied, but loop mediated isothermal amplification (lamp) has not be ... | 2015 | 25842216 |
| a sero-survey of major infectious causes of abortion in small ruminants in morocco. | a serological survey was conducted to estimate the seroprevalence of 5 major abortive infections in 13 sheep flocks and 10 goat herds in 2 regions of morocco. a total of 308 from aborted females (202 ewes and 106 does) and 197 sera (97 ewes and 99 does), were tested for brucellosis, chlamydiosis, q fever, toxoplasmosis, and for 9 major serovars of leptospira. an average abortion rate of 12.10% was found in ewes and 10.26% in does. the serological analyses revealed the presence of all 5 abortive ... | 2015 | 25842210 |
| a probably minor role for land-applied goat manure in the transmission of coxiella burnetii to humans in the 2007-2010 dutch q fever outbreak. | in 2007, q fever started to become a major public health problem in the netherlands, with small ruminants as most probable source. in order to reduce environmental contamination, control measures for manure were implemented because of the assumption that manure was highly contaminated with coxiella burnetii. the aims of this study were 1) to clarify the role of c. burnetii contaminated manure from dairy goat farms in the transmission of c. burnetii to humans, 2) to assess the impact of manure st ... | 2015 | 25816149 |
| impact of serology and molecular methods on improving the microbiologic diagnosis of infective endocarditis in egypt. | conventional diagnosis of infective endocarditis (ie) is based mainly on culture-dependent methods that may fail because of antibiotic therapy or fastidious microorganisms. | 2015 | 25808262 |
| rainfall and sloth births in may, q fever in july, cayenne, french guiana. | q fever in french guiana is correlated with the rainy season. we found a 1- to 2-month lagged correlation between q fever incidence and the number of births of three-toed sloth. this result strengthens the hypothesis that the three-toed sloth is the wild reservoir of q fever in french guiana. | 2015 | 25802429 |
| bulk tank milk surveillance as a measure to detect coxiella burnetii shedding dairy goat herds in the netherlands between 2009 and 2014. | in the period from 2005 to 2009, coxiella burnetii was a cause of abortion waves at 28 dairy goat farms and 2 dairy sheep farms in the netherlands. two years after the first abortion waves, a large human q fever outbreak started mainly in the same region, and aborting small ruminants were regarded as most probable source. to distinguish between infected and noninfected herds, a surveillance program started in october 2009, based on pcr testing of bulk tank milk (btm) samples, which had never bee ... | 2015 | 25795490 |
| cell-free propagation of coxiella burnetii does not affect its relative virulence. | q fever is caused by the obligate intracellular bacterium coxiella burnetii. in vitro growth of the bacterium is usually limited to viable eukaryotic host cells imposing experimental constraints for molecular studies, such as the identification and characterisation of major virulence factors. studies of pathogenicity may benefit from the recent development of an extracellular growth medium for c. burnetii. however, it is crucial to investigate the consistency of the virulence phenotype of strain ... | 2015 | 25793981 |
| latent q fever endocarditis in patients undergoing routine valve surgery. | q fever is a worldwide zoonosis caused by a fastidious bacterium, coxiella burnetii. a recent major outbreak of which in the netherlands will most likely lead to the emergence of hundreds of cases of c. burnetii endocarditis during the next decade. patients undergoing cardiac valve surgery may carry undiagnosed q fever endocarditis with possible disastrous outcomes, and hence may benefit from a screening strategy. the study aim was to evaluate the frequency of unsuspected latent q fever endocard ... | 2014 | 25790621 |
| q fever infection in dairy cattle herds: increased risk with high wind speed and low precipitation. | ruminants are considered the main reservoir for transmission of coxiella burnetii (cb) to humans. the implementation of effective control measures against cb in ruminants requires knowledge about potential risk factors. the objectives of this study were (i) to describe the spatial distribution of q fever-infected dairy cattle herds in sweden, (ii) to quantify the respective contributions of wind and animal movements on the risk of infection, while accounting for other sources of variation, and ( ... | 2015 | 25783480 |
| seroprevalence of coxiella burnetii in domesticated and feral cats in eastern australia. | the seroprevalence of coxiella burnetii (c. burnetii) in cats in eastern australia is unknown, and the risk of transmission from cats to humans is undetermined. this study aimed to determine the exposure of cats to c. burnetii in four distinct cat subpopulations. an indirect immunofluoresence assay (ifa) and an enzyme-linked immunosorbent assay (elisa) used for detection of anti-c. burnetii antibodies in humans were adapted, verified for use on feline serum, and compared. cat serum samples (n=71 ... | 2015 | 25778545 |
| occurrence of coxiella burnetii and chlamydiales species in abortions of domestic ruminants and in wild ruminants in hungary, central europe. | coxiella burnetii and certain members of the chlamydiales order are zoonotic, intracellular, gram-negative bacteria, with abortigenic potential in ruminants. these pathogens have a broad host range and worldwide geographical distribution. the current study aimed to reveal the importance of c. burnetii and chlamydiales spp. in abortions in domestic ruminants and their occurrence in wild ruminants with real-time polymerase chain reaction (qpcr) assays, histology, and immunohistochemical staining ( ... | 2015 | 25776545 |
| familial q fever clustering with variable manifestations imitating infectious and autoimmune disease. | q fever, caused by coxiella burnetii, can present as an outbreak of acute disease ranging from asymptomatic disease, pneumonia, hepatitis or fever of unknown origin, which can progress to a chronic disease, most frequently endocarditis. the occurrence of q fever within families is rarely described, and in most cases presents with uniform acute disease manifestations. here we present a familial cluster of q fever presenting as highly variable synchronous manifestations in four of five family memb ... | 2015 | 25770747 |