Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| bacterial vaccines for fish--an update of the current situation worldwide. | during the last few years, the use of vaccines for disease prevention in aquaculture has expanded both with regard to the number of fish species and number of microbial diseases. according to the responses to a questionnaire received from 41 countries, vaccination is used in the commercial aquaculture of species like atlantic salmon (salmo salar), rainbow trout (oncorhynchus mykiss), sea bass (dicentrarchus labrax), sea bream (sparus aurata), barramundi (lates calcarifer), tilapia (tilapia spp), ... | 2005 | 15962470 |
| conjugal vectors for cloning, expression, and insertional mutagenesis in gram-negative bacteria. | 2005 | 15945369 | |
| interactive toxic effects of heavy metals and humic acids on vibrio fischeri. | the effect of humic acids (has) on the toxicity of copper, zinc, and lead was investigated using the photobacterium vibrio fischeri (microtox test) as a test organism. the effects of has on metal toxicity were evaluated as functions of time and concentration in pure compound solutions. the toxicities of copper and lead were generally comparable, while the toxicity of zinc was lower than those of the other two metals. the toxicity of copper decreased with the addition of has, while the toxicity o ... | 2006 | 15939470 |
| [conditions that influence bacterial luminescence in the presence of blood serum]. | conditions that influence the luminescence of natural and recombinant luminescent bacteria in the presence of blood serum were studied. in general, blood serum quenched the luminescence of the marine photobacterium phosphoreum and the recombinant escherichia coli strains harboring the luminescent system genes of photobacterium leiognathi, but enhanced the luminescence of the soil bacterium photorhabdus luminescens zm1 and the recombinant e. coli strain harboring the lux operon of p. luminescens ... | 2005 | 15938395 |
| a simple hydrophobicity-based approach to predict the toxicity of unknown organic micropollutant mixtures in marine water. | in this study, we propose a simple hydrophobicity-based approach to predict the toxicity of mixtures of marine organic micropollutants. fifty mixtures were chosen, comprising individual chemicals randomly chosen from three halogenated benzenes, three phenols, two petroleum hydrocarbons, three polychlorinated biphenyls (pcbs), four organochlorine pesticides (ocps) and two herbicides, with the intention of simulating the mixtures of organic micropollutants that may exist in some marine waters. the ... | 2005 | 15935177 |
| significant histamine formation in tuna (thunnus albacares) at 2 degrees c--effect of vacuum- and modified atmosphere-packaging on psychrotolerant bacteria. | occurrence and importance of psychrotolerant histamine producing bacteria in chilled fresh tuna were demonstrated in the present study. the objective was to evaluate microbial formation of histamine and biogenic amines in chilled fresh tuna from the indian ocean and stored either vacuum-packed (vp) or modified atmosphere-packed (map). firstly, biogenic amines and the dominating microbiota were determined in vp tuna involved in an outbreak of histamine fish poisoning in denmark. secondly, the mic ... | 2005 | 15925710 |
| characteristics of endogenous flavin fluorescence of photobacterium leiognathi luciferase and vibrio fischeri nad(p)h:fmn-oxidoreductase. | the bioluminescent bacterial enzyme system nad(p)h:fmn-oxidoreductase-luciferase has been used as a test system for ecological monitoring. one of the modes to quench bioluminescence is the interaction of xenobiotics with the enzymes, which inhibit their activity. the use of endogenous flavin fluorescence for investigation of the interactions of non-fluorescent compounds with the bacterial luciferase from photobacterium leiognathi and nad(p)h:fmn-oxidoreductase from vibrio fischeri has been propo ... | 2005 | 15924327 |
| heme uptake genes in human and fish isolates of photobacterium damselae: existence of huta pseudogenes. | the marine bacterium photobacterium damselae includes strains classified into two distinct subspecies, namely subsp. damselae and subsp. piscicida, which have been reported to cause disease in a variety of marine animals and in humans. p. damselae strains utilize heme compounds as sole iron sources. in the present study, ten potential heme uptake and utilization genes are described in p. damselae subsp. damselae and subsp. piscicida. one gene cluster includes the genes coding for putative protei ... | 2005 | 15918073 |
| the effect of sea bream (sparus aurata) broodstock and larval vaccination on the susceptibility by photobacterium damsela subsp. piscicida and on the humoral immune parameters. | sea bream broodstock were immunised 1 or 2 months before spawning with a novel photobacteriosis vaccine. sixty-seven-day-old larvae (mean weight 22.3 mg) originating from immunised and non-immunised parents were experimentally infected with the photobacterium damsela subsp. piscicida (phdp). larvae from immunised fish showed delayed onset and lower mortality (66.67%) compared with larvae from control fish (80%). eighty-nine-day-old larvae (mean weight 162.2 mg) from both groups were bath vaccina ... | 2005 | 15863015 |
| adhesion to sole, solea senegalensis kaup, mucus of microorganisms isolated from farmed fish, and their interaction with photobacterium damselae subsp. piscicida. | abstract most studies carried out to select microorganisms as candidate probiotics have focused on in vitro antagonism tests, such as the production of inhibitory compounds against pathogenic microorganisms. however, attachment to mucous surfaces could be another criterion to be considered when selecting potential probiotics for aquaculture. nineteen isolates obtained from farmed senegalese sole, solea senegalensis kaup, and gilthead sea bream, sparus aurata l., have been evaluated for their cap ... | 2005 | 15813865 |
| nonbioluminescent strains of photobacterium phosphoreum produce the cell-to-cell communication signal n-(3-hydroxyoctanoyl)homoserine lactone. | bioluminescence is a common phenotype in marine bacteria, such as vibrio and photobacterium species, and can be quorum regulated by n-acylated homoserine lactones (ahls). we extracted a molecule that induced a bacterial ahl monitor (agrobacterium tumefaciens nt1 [pzlr4]) from packed cod fillets, which spoil due to growth of photobacterium phosphoreum. interestingly, ahls were produced by 13 nonbioluminescent strains of p. phosphoreum isolated from the product. of 177 strains of p. phosphoreum (i ... | 2005 | 15812045 |
| [characterization of microbial population present in the edible seaweed, monostroma undulatum, wittrock]. | the microbiological quality of monostroma undulatum, wittrock from the southern argentinean coast, was studied for its application for human food. also the diversity and function of the native bacterial population to this green seaweed was analyzed. samples were collected in puerto deseado, province of santa cruz, southern argentina (47 degrees 45'l.s., 65 degrees 55'l.w). the samples were analyzed for the presence of psycotrophic heterotrophic bacteria, marine heterotrophic bacteria, low nutrit ... | 2004 | 15807211 |
| biolumbase--the database of natural and transgenic bioluminescent organisms. | the institute of biophysics sb ras hosts and maintains a specialized collection of luminous bacteria (ccibso 836) containing over 700 strains isolated in various regions of the world's oceans. the culture collection is a source of lux genes and biologically active substances. the wide application of bioluminescence in medicine and ecology has given importance to analysing information on the structure and functioning of bioluminescence systems in natural and transgenic microorganisms, as well as ... | 2005 | 15803502 |
| immobilization as a technical possibility for long-term storage of bacterial biosensors. | for applications in field experiments, the recombinant strain salmonella typhimurium ta1535 was immobilized to permit its immediate utilization after long storage periods. salmonella typhimurium ta1535 cells contain the plasmid that has an inducible sos promoter fused to a promoterless luxcdabfe operon from photobacterium leiognathi. the induction of bioluminescence occurs in the presence of the dna-damaging agent mitomycin c which stimulates the bacterial sos response. early stationary phase ce ... | 2005 | 15791471 |
| antimicrobial substances from rhizomes of the giant knotweed polygonum sachalinense against the fish pathogen photobacterium damselae subsp. piscicida. | the antimicrobial compounds against the fish pathogen photobacterium damselae subsp. piscicida were isolated from polygonum sachalinense rhizomes. the structures of the antimicrobial compounds 1 and 2 were determined by 1h and 13c nmr, 2d-nmr (cosy, hsqc, hmbc and roesy) and fab-ms to be phenylpropanoid glycosides, vanicoside a and b, respectively. both compounds have feruloyl and p-coumaroyl groups bonded to a sucrose moiety in their structures. vanicoside a also has an acetyl group in the sucr ... | 2005 | 15787242 |
| photobacterium rosenbergii sp. nov. and enterovibrio coralii sp. nov., vibrios associated with coral bleaching. | six new vibrio-like isolates originating from different species of bleached and healthy corals around magnetic island (australia) were investigated using a polyphasic approach. phylogenetic analyses based on 16s rrna, reca and rpoa gene sequences split the isolates in two new groups. strains lmg 22223(t), lmg 22224, lmg 22225, lmg 22226 and lmg 22227 were phylogenetic neighbours of photobacterium leiognathi lmg 4228(t) (95.6 % 16s rrna gene sequence similarity), whereas strain lmg 22228(t) was r ... | 2005 | 15774685 |
| toxicity of agricultural dust extract using microtox. | 2005 | 15768505 | |
| life at depth: photobacterium profundum genome sequence and expression analysis. | deep-sea life requires adaptation to high pressure, an extreme yet common condition given that oceans cover 70% of earth's surface and have an average depth of 3800 meters. survival at such depths requires specific adaptation but, compared with other extreme conditions, high pressure has received little attention. recently, photobacterium profundum strain ss9 has been adopted as a model for piezophily. here we report its genome sequence (6.4 megabase pairs) and transcriptome analysis. the result ... | 2005 | 15746425 |
| characterization of fe/mn-superoxide dismutase from diatom thallassiosira weissflogii: cloning, expression, and property. | a cdna clone of 1114 bp encoding a putative mn-superoxide dismutase (mn-sod) from diatom thallassiosira weissflogii was cloned by the pcr technique. nucleotide sequence analysis of this cdna clone revealed that it was translated into 201 amino acid residues. when the sequence was compared with mn-sods from vibrio mimicus and escherichia coli, as well as two fe-sods from e. coli and photobacterium leiognathi, this sod showed higher homology to mn-sod. the amino acid residues required to coordinat ... | 2005 | 15740026 |
| side effects in sea bass (dicentrarchus labrax l.) due to intraperitoneal vaccination against vibriosis and pasteurellosis. | sea bass (dicentrarchus labrax l.) were injected intraperitoneally with monovalent (photobacterium damselae subsp. piscicida or vibrio anguillarum) and divalent (ph. damselae subsp. piscicida and v. anguillarum) vaccine formulations, with or without adjuvants (mineral oil, liposome or alginate), to evaluate the short and long-term pathological effects. eight animals from each group were sampled one, two, four and 11 months after intraperitoneal injection. the acute peritoneal response and the pr ... | 2005 | 15722227 |
| sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species. | recent studies indicated that bioluminescence of the marine bacterium vibrio harveyi may both stimulate dna repair and contribute to detoxification of deleterious oxygen derivatives. therefore, it was also proposed that these reactions can be considered biological roles of bacterial luminescence and might act as evolutionary drives in development of luminous systems. however, experimental evidence for the physiological role of luciferase in protection of cells against oxidative stress has been d ... | 2005 | 15717160 |
| molecular cloning and functional analysis of photobacterium damselae subsp. piscicida haem receptor gene. | a haem receptor gene from photobacterium damselae subsp. piscicida (formerly known as pasteurella piscicida) has been cloned, sequenced and analysed for its function. the gene, designated as pph, has an open reading frame consisting of 2154 bp, a predicted 718 amino acid residues and exists as a single copy. it is homologous with the haem receptors of vibrio anguillarum hupa, v. cholerae huta, v. mimicus mhua and v. vulnificus hupa at 32.7, 32.7, 45.6 and 30.9%, respectively, and is highly conse ... | 2005 | 15705153 |
| genomic and phylogenetic characterization of luminous bacteria symbiotic with the deep-sea fish chlorophthalmus albatrossis (aulopiformes: chlorophthalmidae). | bacteria forming light-organ symbiosis with deep-sea chlorophthalmid fishes (aulopiformes: chlorophthalmidae) are considered to belong to the species photobacterium phosphoreum. the identification of these bacteria as p. phosphoreum, however, was based exclusively on phenotypic traits, which may not discriminate between phenetically similar but evolutionarily distinct luminous bacteria. therefore, to test the species identification of chlorophthalmid symbionts, we carried out a genomotypic (repe ... | 2005 | 15691950 |
| variation in 16s-23s rrna intergenic spacer regions in photobacterium damselae: a mosaic-like structure. | phenotypically, photobacterium damselae subsp. piscicida and p. damselae subsp. damselae are easily distinguished. however, their 16s rrna gene sequences are identical, and attempts to discriminate these two subspecies by molecular tools are hampered by their high level of dna-dna similarity. the 16s-23s rrna internal transcribed spacers (its) were sequenced in two strains of photobacterium damselae subsp. piscicida and two strains of p. damselae subsp. damselae to determine the level of molecul ... | 2005 | 15691912 |
| identification of fur regulated genes in the bacterial fish pathogen photobacterium damselae ssp. piscicida using the fur titration assay. | bacteria have developed a series of iron-scavenging and transport systems. the expression of many of the iron utilization genes is tightly regulated by the fe2+ loaded fur repressor protein. in this study, the fur titration assay (furta) was used to screen for dna fragments from a genomic dna library of photobacterium damselae ssp. piscicida containing potential fe2+ fur binding sites or iron binding-proteins which withdraw iron from fur. one of the clones encoded a tonb gene and adjacent a func ... | 2004 | 15689115 |
| experimental and simulative dissociation of dimeric cu,zn superoxide dismutase doubly mutated at the intersubunit surface. | the equilibrium properties of dimeric photobacterium leiognathi cu,zn superoxide dismutase mutant bearing two negative charges in the amino acid clusters at the association interface has been studied, experimentally and computationally, and compared to those of the native enzyme. pressure-dependent dissociation is observed for the mutant, as observed by the fluorescence shift of the unique tryptophan residue located at the intersubunit surface. the spectral shift occurs slowly, reaching a platea ... | 2005 | 15681652 |
| [a primary study on antimicrobial and cytotoxic activity of marine bacteria]. | marine bacteria were isolated from marine organisms including invertebrates and seaweeds and from sea water and sediments of different sea area. ethyl acetate extractions of marine bacterial fermentation were screened for antimicrobial and cytotoxicity activities by using the methods of agar diffusion and mtt assay, and the relationship of active bacteria and their origin was studied. the results showed that 42 strains among total 341 isolated marine bacteria had antimicrobial activity, and 7 st ... | 2004 | 15669499 |
| influence of hydroxypropylcyclodextrins on the toxicity of mixtures. | we studied the influence of hydroxypropylcyclodextrins (hpcds) on the toxicity of some mixtures. using the photobacterium phosphoreum toxicity test, the joint toxicological effect for mixture i (containing p-nitrobenzaldehyde and 1-nitronaphthalene) and mixture ii (containing p-nitrobenzaldehyde and malononitrile) were determined in water and in aqueous solutions of hpcds. the results indicate that, although the toxicological joint effect for mixture i (simple addition) differs from that of mixt ... | 2005 | 15667850 |
| effect of human serum on bioluminescence of natural and recombinant luminescent bacteria. | biphasic modification of bacterial bioluminescence by human serum was revealed: bioluminescence was inhibited at high concentrations of the serum and stimulated at low concentrations. effects of temperature and duration of exposure on bioluminescence manifested in stimulation of the inhibitory effect at higher temperature and longer exposure. the degree of inhibition of bioluminescence under in the presence of serum depends on species characteristics of the microorganism and nature of the lumine ... | 2004 | 15665923 |
| effectiveness of a divalent vaccine for sole, solea senegalensis (kaup), against vibrio harveyi and photobacterium damselae subsp. piscicida. | the protection of cultured sole, solea senegalensis, against vibrio harveyi and photobacterium damselae subsp. piscicida was evaluated following the use of a divalent vaccine prepared with formalized whole cells and extracellular products of virulent strains of both pathogenic microorganisms and administered by the immersion route. two prolonged immersions of 5-10 g fish in the divalent bacterin at a 1-month interval gave high levels of protection similar to those obtained when the respective mo ... | 2005 | 15660791 |
| photobacterium lipolyticum sp. nov., a bacterium with lipolytic activity isolated from the yellow sea in korea. | a gram-negative, motile, non-spore-forming, pleomorphic and lipolytic bacterial strain, m37t, was isolated from an intertidal sediment of the yellow sea in korea. this organism grew optimally at 25-28 degrees c and in the presence of 1-2 % nacl. it did not grow without nacl or in the presence of more than 6 % nacl. strain m37t was characterized chemotaxonomically by having q-8 as the predominant respiratory lipoquinone and c(16 : 1)omega7c and/or iso-c(15 : 0) 2-oh and c(16 : 0) as the major fat ... | 2005 | 15653897 |
| dark variants of luminous bacteria whole cell bioluminescent optical fiber sensor to genotoxicants. | a stable dark variant separated from photobacterium phosphoreum (a2) was fixed in agar-gel membrane and immobilized onto an exposed end of a fiber-optic linked with bioluminometer. the variant could emit a luminescent signal in the presence of genotoxic agents, such as mitomycin c (mc). the performance of this whole-cell optical fiber sensor system was examined as a function of several parameters, including gel probe thickness, bacterial cell density, and diameter of the fiber-optic core and wor ... | 2004 | 15641706 |
| genome update: proteome comparisons. | 2005 | 15632419 | |
| characteristic analysis of the ampc gene encoding beta-lactamase from photobacterium phosphoreum. | the ampc gene of photobacterium phosphoreum atcc 11040 was cloned and identified. nucleotide sequence of the regulatory region r&r and the ampc gene (genbank accession no. ay787792) from p. phosphoreum has been determined, and the encoded beta-lactamase is deduced. the beta-lactamase encoded by the ampc gene has a calculated m(r) 31,198 and comprises 285 amino acid residues (pi 7.35). there is a signal peptide of 20 amino acid residues mklrfiastlllsfsqlasa to lead the beta-lactamase secretion, a ... | 2005 | 15596133 |
| characterization of the 23s and 5s rrna genes and 23s-5s intergenic spacer region (its-2) of photobacterium damselae. | the 23s ribosomal rna (rrna) gene has been sequenced in strains of the fish pathogens photobacterium damselae subsp. damselae (atcc 33539) and subsp. piscicida (atcc 29690), showing that 3 nucleotide positions are clearly different between subspecies. in addition, the 5s rrna gene plus the intergenic spacer region between the 23s and 5s rrna genes (its-2) were amplified, cloned and sequenced for the 2 reference strains as well as the field isolates rg91 (subsp. damselae) and di21 (subsp. piscici ... | 2004 | 15584408 |
| transfer of hyphomicrobium indicum to the genus photobacterium as photobacterium indicum comb. nov. | hyphomicrobium indicum johnson and weisrock 1969 lacks true budding and hyphal branching, and some phenotypic characteristics are in contrast to other true hyphomicrobia. the major quinone system (ubiquinone q-8), the g+c content of the dna (40 mol%) and the cellular fatty acid composition (16 : 0, 16 : 1 and 18 : 1 as the major components, and 12 : 0 3-oh and 14 : 0 3-oh as the hydroxy fatty acids) of h. indicum are different from the genus hyphomicrobium, but similar to the genus photobacteriu ... | 2004 | 15545443 |
| effect of dietary iron overload on photobacterium damselae ssp. piscicida pathogenicity in sea bass, dicentrarchus labrax (l.). | 2004 | 15509262 | |
| [effect of glucose on luciferase expression in photobacterium leiognathi]. | photobacterium leiognathi cultured in marine broth emits a luminescence that is temporarily enhanced and then extinguished by glucose. glucose reduces the luciferase level and the expression of lux abg mrna in p. leiognathi. the amount of atp in p. leiognathi is temporarily increased and then declines to the normal level. these results indicate that the extinguishing by glucose in p. leiognathi is induced by the interruption of the translation of luciferase. | 2004 | 15467278 |
| coregulation of lux genes and riboflavin genes in bioluminescent bacteria of photobacterium phosphoreum. | investigation of the expression of the riboflavin (rib) genes, which are found immediately downstream of luxg in the lux operon in photobacterium phosphoreum, provides more information relevant to the evolution of bioluminescence, as well as to the regulation of supply of flavin substrate for bacterial bioluminescence reactions. in order to answer the question of whether or not the transcriptions of lux and rib genes are integrated, a transcriptional termination assay was performed with p. phosp ... | 2004 | 15459647 |
| influence of vaccination on the nitric oxide response of gilthead seabream following infection with photobacterium damselae subsp. piscicida. | the nitric oxide (no) response of vaccinated and non-vaccinated juvenile gilthead seabream was studied in vivo and the no response of isolated kidney macrophages of fish was studied in vitro. fish were vaccinated with formalin-killed photobacterium damselae subsp. piscicida (pdp) with or without freund's incomplete adjuvant (fia) and control fish received phosphate buffered saline (pbs). thirty days later, fish were injected with a sublethal dose of pdp and 3 fish/group were bled at time periods ... | 2005 | 15450966 |
| distinctive functional features in prokaryotic and eukaryotic cu,zn superoxide dismutases. | bacterial and eukaryotic cu,zn superoxide dismutases show remarkable differences in the active site region and in their quaternary structure organization. we report here a functional comparison between four cu,zn superoxide dismutases from gram-negative bacteria and the eukaryotic bovine enzyme. our data indicate that bacterial dimeric variants are characterized by catalytic rates higher than that of the bovine enzyme, probably due to the solvent accessibility of their active site. prokaryotic c ... | 2004 | 15449711 |
| a purine-requiring strain of photobacterium fischeri. | 1949 | 15393696 | |
| phylogenetic relationships among marine alteromonas-like proteobacteria: emended description of the family alteromonadaceae and proposal of pseudoalteromonadaceae fam. nov., colwelliaceae fam. nov., shewanellaceae fam. nov., moritellaceae fam. nov., ferrimonadaceae fam. nov., idiomarinaceae fam. nov. and psychromonadaceae fam. nov. | the phylogenetic relationships among marine alteromonas-like bacteria of the genera alteromonas, pseudoalteromonas, glaciecola, thalassomonas, colwellia, idiomarina, oceanimonas, oceanisphaera, shewanella, moritella, ferrimonas, psychromonas and several other genera of the 'gammaproteobacteria' were studied. results of 16s rrna gene sequence analyses revealed that some members of these genera formed several coherent groups at the family level. characteristic signature oligonucleotides for studie ... | 2004 | 15388743 |
| biodiversity of vibrios. | vibrios are ubiquitous and abundant in the aquatic environment. a high abundance of vibrios is also detected in tissues and/or organs of various marine algae and animals, e.g., abalones, bivalves, corals, fish, shrimp, sponges, squid, and zooplankton. vibrios harbour a wealth of diverse genomes as revealed by different genomic techniques including amplified fragment length polymorphism, multilocus sequence typing, repetetive extragenic palindrome pcr, ribotyping, and whole-genome sequencing. the ... | 2004 | 15353563 |
| random mutagenesis of bacterial luciferase: critical role of glu175 in the control of luminescence decay. | bacterial luciferases (luxab) can be readily classed as slow or fast decay luciferases based on their rates of luminescence decay in a single turnover assay. luciferases from vibrio harveyi and xenorhabdus (photorhabdus) luminescens have slow decay rates, and those from the photobacterium genus, such as photobacterium fisheri, p. phosphoreum and p. leiognathi, have rapid decay rates. by substitution of a 67-amino-acid stretch of p. phosphoreum luxa in the central region of the luxa subunit, the ... | 2005 | 15352872 |
| activity of two histidine decarboxylases from photobacterium phosphoreum at different temperatures, phs, and nacl concentrations. | the major causative agent of scombroid poisoning is histamine formed by bacterial decarboxylation of histidine. the authors reported previously that histamine was exclusively formed by the psychrotrophic halophilic bacteria photobacterium phosphoreum in scombroid fish during storage at or below 10 degrees c. moreover, histamine-forming ability was affected by two histidine decarboxylases: constitutive and inducible enzymes. this article reports the effect of various growth and reaction condition ... | 2004 | 15330542 |
| [influential factors on the toxicity of pentachlorophenol sodium with microtox system in water]. | the biological toxicity of pentachlorophenol sodium (na-pcp), a typical kind of aquatic organic pollutants, was tested with the microtox system using luminescent bacteria with the influence of several factors taken into consideration. the ec50 of na-pcp increases with the increasing of the ph and hardness of the sample. the ec50 (15 min) of na-pcp is approximately equal to its ec50 (20 min). compared with some common organic pollutants, which exhibit little influence on the toxicity of na-pcp wh ... | 2004 | 15327251 |
| mercury chloride effects on the function and cellular integrity of sea bass (dicentrarchus labrax) head kidney macrophages. | the objective of this work was to study mercury chloride effects on the function and integrity of sea bass (dicentrarchus labrax) head kidney macrophages (s-hkm), and to evaluate the response of hgcl2-exposed cells to macrophage activating factor(s) (maf) produced by sea bass head kidney leukocytes. there was considerable variability in the effects of hgcl2 on the production of reactive oxygen species (ros) by s-hkm. when incubated with hgcl2, cells from five out of nine fish tested showed a dec ... | 2004 | 15313514 |
| maternal transfer of humoral specific and non-specific immune parameters to sea bream (sparus aurata) larvae. | immunisation of sea bream (sparus aurata l.) broodstock with a novel vaccine mixture of photobacterium damsela subsp. piscicida sk7 (phdp) was performed during the period of egg development and the changes in specific and non-specific humoral immune parameters were measured. total immunoglobulin level, specific antibody titre, anti-protease activity and lysozyme activity were significantly higher in immunised parents compared to the control. after spawning significantly higher anti-protease acti ... | 2004 | 15313509 |
| structure of flavoprotein fp390 from a luminescent bacterium photobacterium phosphoreum refined at 2.7 a resolution. | the three-dimensional structure of a flavoprotein, fp(390), from a luminescent bacterium, photobacterium phosphoreum, solved by the molecular-replacement method, was refined to an r factor of 24.0% for 17 433 independent reflections, from 6.0 to 2.7 a resolution, collected by synchrotron radiation. the asymmetric unit of the crystal (space group p4(3)22, a = b = 76.8 and c = 242 a) contains two monomer molecules related by a non-crystallographic twofold axis to form a dimer. there are two q-flav ... | 1996 | 15299728 |
| explorative multivariate analyses of 16s rrna gene data from microbial communities in modified-atmosphere-packed salmon and coalfish. | modified-atmosphere packaging (map) of foods in combination with low-temperature storage extends product shelf life by limiting microbial growth. we investigated the microbial biodiversity of map salmon and coalfish by using an explorative approach and analyzing both the total amounts of bacteria and the microbial group composition (both aerobic and anaerobic bacteria). real-time pcr analyses revealed a surprisingly large difference in the microbial loads for the different fish samples. the micr ... | 2004 | 15294842 |
| [the effect of clp proteins on dnak-dependent refolding of bacterial luciferases]. | a study was made of the refolding of bacterial luciferases of vibrio fischeri, v. harveyi, photobacterium phosphoreum, and photorhabdus luminescens. by reaction rate, luciferases were divided into two groups. the reaction rate constants of fast luciferases of v. fischeri and ph. phosphoreum were about tenfold higher than those of slow luciferases of ph. luminescens and v. harveyi. the order of increasing luciferase thermostability was ph. phosphoreum, v. fischeri, v. harveyi, and ph. luminescens ... | 2004 | 15285621 |
| occurrence of tetracycline resistance genes tet(m) and tet(s) in bacteria from marine aquaculture sites. | occurrence of tetracycline resistance genes encoding ribosomal protection proteins was examined in 151 tetracycline-resistant bacterial isolates from fish and seawater at coastal aquaculture sites in japan and korea. the tet(m) gene was detected in 34 japanese and korean isolates, which included vibrio sp., lactococcus garvieae, photobacterium damsela subsp. piscicida, and unidentified gram-positive bacteria. the majority of these bacterial isolates displayed high-level resistance with a minimum ... | 2004 | 15268950 |
| a new approach to the problem of weak magnetic fields: an effect on living objects. | 2003 | 15255202 | |
| retraction. fatal infection of the hand due to photobacterium damsela: a case report. | 2004 | 15224696 | |
| comparative toxicological evaluation of untreated and treated tannery effluent with nostoc muscorum l. (algal assay) and microtox bioassay. | the relative sensitivity of tannery effluent before and after treatment was compared by employing nostoc muscorum and microtox assay in laboratory. the effect on chlorophyll, protein and biomass content of nostoc muscorum was studied with the luminescent property inhibition of photobacterium phosphorium and compared with algal bioassay. the results of microtox assay after 5, 15 and 30 min of exposure were compared with data obtained from algal bioassay. it was observed that the luminescent prope ... | 2004 | 15195832 |
| effects of hydrogen peroxide on light emission by various strains of marine luminescent bacteria. | light-emitting bacteria are the most abundant and widespread luminescent organisms. most species of such bacteria live in marine environments. however, until recently, biological role of bacterial luminescence remained unknown. recent studies indicated that light produced in bacterial cells may stimulate dna repair. therefore, it is not surprising that agents that cause dna damage induce expression of lux genes. moreover, it was proposed previously that bacterial luciferases may be involved in d ... | 2004 | 15162391 |
| fatal infection of the hand due to photobacterium damsela: a case report. | we report a case of fatal acute renal failure due to photobacterium damsela infection of the hand. although the patient received intensive medical care and surgical treatment, he died 8 h after arrival at the hospital. this is the first case of p. damsela sepsis proven by blood culture in japan. | 2004 | 15156501 |
| use of reca as an alternative phylogenetic marker in the family vibrionaceae. | this study analysed the usefulness of reca gene sequences as an alternative phylogenetic and/or identification marker for vibrios. the reca sequences suggest that the genus vibrio is polyphyletic. the high heterogeneity observed within vibrios was congruent with former polyphasic taxonomic studies on this group. photobacterium species clustered together and apparently nested within vibrios, while grimontia hollisae was apart from other vibrios. within the vibrios, vibrio cholerae and vibrio mimi ... | 2004 | 15143042 |
| the effect of growth medium salinity of photobacterium damselae subsp. piscicida on the immune response of hybrid bass (morone saxatilis x m. chrysops). | photobacterium damselae subsp. piscicida (p. damselae) was grown on various media and the effect of media salinity on certain immune responses of hybrid bass was studied. in israel, pasteurellosis outbreaks have not been reported at water salinities below 1.38 per thousand. during vaccination experiments the salinity of the medium on which p. damselae is grown, was shown to affect stimulation of the immune system. no correlation was found between antibody response and protection. bacterial envel ... | 2004 | 15123315 |
| ion trap lc/ms characterisation of toxic polar organic pollutants in colour photographic wastewaters and monitoring of their chemical degradation. | liquid chromatography/electrospray ionisation-ion trap mass spectrometry (lc/esi-itms) with positive mode of operation was successfully applied to the characterisation of aromatic amines and chelating agents in colour photographic wastewaters. in addition to residual ingredients, monomers and dimers of sulphonated aromatic amines were the main toxic polar organic pollutants found. oxidation of wastewater components by the fenton-like reagent (fe3+ + h2o2) was investigated by continuously pumping ... | 2004 | 15116875 |
| activation of the nitric oxide response in gilthead seabream after experimental infection with photobacterium damselae subsp. piscicida. | inoculation of small gilthead seabream (sparus aurata) (30-75 g body weight) with a sublethal dose of different photobacterium damselae subsp. piscicida (pdp) strains (di-21 and 94/99) induced an increase in serum concentrations of stable nitric oxide (no) metabolites lasting from 6 h to six days post-infection, with a peak at 24 h. in contrast, no such response was detected in larger fish (150-600 g). since the virulence of pdp correlates with the presence of a polysaccharide capsular layer whi ... | 2004 | 15110332 |
| identification and characterisation of the fur genes in photobacterium damselae ssp. piscicida and ssp. damselae. | the gene encoding the ferric uptake regulator protein (fur gene) of the fish pathogenic bacterium photobacterium damselae ssp. piscicida strain d121 was partially amplified using degenerate oligonucleotides. complete sequencing of the fur gene and neighbouring dna was accomplished by primer walking. an open reading frame of 447 bp, coding for a protein of 148 amino acids, and with high homology to previously described fur proteins, was identified. the fur gene of p. damselae ssp. damselae atcc 3 ... | 2004 | 15109136 |
| chemical speciation and toxicity of metals assessed by three bioluminescence-based assays using marine organisms. | metal toxicity is a function of the biology of the target organism and the chemical speciation of the metal. the toxicity of 11 metals was assessed with three cell-based bioassays based on marine organisms: the bacterium photobacterium phosphoreum of the microtox bioassay, an environmental strain of p. phosphoreum, and photocytes isolated from the brittlestar ophiopsila californica. metal speciation was calculated for three commonly used media: nacl-based microtox bioassay medium, artificial sea ... | 2004 | 15101032 |
| continuous culture of photobacterium. | the design and performance characteristics of a small volume (20 ml) continuous culture device for the cultivation of luminous bacteria are described. this simply constructed device can be used to supply luminescent bacteria with constant properties for either laboratory use or the assay of environmental pollutants. furthermore, bacteria can be deployed to make sensitive (<1 nm) oxygen measurements. the culture device may be configured, alone or in combination, as a chemostat, turbidostat or a " ... | 2004 | 15093217 |
| mutatox test: a new test for monitoring environmental genotoxic agents. | in this study, yamaska river water and milli-q water and organically extracted sediment extracts were used to evaluate the sensitivity of a new genotoxicity screening test, the mutatox test. also in this study, the samples were tested for acute and chronic toxicity using the following screening test procedures: microtox, daphnia magna, ceriodaphnia reticulata and atp-tox systems. the mutatox test is based on the use of a dark mutant strain of photobacterium phosphoreum and is sensitive to chemic ... | 1990 | 15092256 |
| biosynthesis and dietary uptake of polyunsaturated fatty acids by piezophilic bacteria. | the biochemistry of piezophilic bacteria is unique in that piezophiles produce polyunsaturated fatty acids (pufas). a pertinent question is if piezophilic bacteria synthesize pufa de novo, through dietary uptake, or both. this study was undertaken to examine the biosynthesis and cellular uptake of pufas by piezophilic bacteria. a moderately piezophilic (shewanella violacea dss12) and two hyperpiezophilic bacteria (s. benthica db21mt-2 and moritella yayanosii db21mt-5) were grown under 50 mpa (me ... | 2004 | 15081997 |
| simultaneous detection of marine fish pathogens by using multiplex pcr and a dna microarray. | we coupled multiplex pcr and a dna microarray to construct an assay suitable for the simultaneous detection of five important marine fish pathogens (vibrio vulnificus, listonella anguillarum, photobacterium damselae subsp. damselae, aeromonas salmonicida subsp. salmonicida, and vibrio parahaemolyticus). the array was composed of nine short oligonucleotide probes (25-mer) complementary to seven chromosomal loci (cyt, rpon, gyrb, toxr, urec, dly, and vapa) and two plasmid-borne loci (fata and a.sa ... | 2004 | 15070982 |
| cloning and characterization of the gene encoding for omp-pd porin: the major photobacterium damsela outer membrane protein. | the outer membrane protein of photobacterium damsela (omp-pd) and the gene encoding for this porin protein were isolated and characterized. the deduced amino acid sequence of the omp-pd monomer has 338 amino acids and a calculated molecular weight of 36,951 da. this sequence includes a 22-amino acid signal peptide at the n-terminal, which is not found when the monomer is located in the outer membrane. native omp-pd protein forms a trimeric structure of approximately 110 kda. it exhibits resistan ... | 2004 | 15057460 |
| phylogenetic analysis of the lux operon distinguishes two evolutionarily distinct clades of photobacterium leiognathi. | the luminous marine bacterium photobacterium mandapamensis was synonymized several years ago with photobacterium leiognathi based on a high degree of phenotypic and genetic similarity. to test the possibility that p. leiognathi as now formulated, however, actually contains two distinct bacterial groups reflecting the earlier identification of p. mandapamensis and p. leiognathi as separate species, we compared p. leiognathi strains isolated from light-organ symbiosis with leiognathid fishes (i.e. ... | 2004 | 15034641 |
| photobacterium phosphoreum caused a histamine fish poisoning incident. | an incident of histamine fish poisoning (hfp) occurred due to the consumption of iwashi maruboshi (dried sardine) in osaka, japan in march 2002. a histamine-producing bacterial strain, ys4-7, was isolated from iwashi maruboshi that contained 1700 mg of histamine per kilogram. this strain was identified as photobacterium phosphoreum by biochemical examinations and partial sequencing of 16s rdna. p. phosphoreum ys4-7 showed greater capability as a histamine producer at 4 and 12 degrees c than morg ... | 2004 | 15033270 |
| development of a fragment constant method for estimating the mixture toxicity. | based on group contribution theory, a fragment constant model to estimate mixture toxicity is proposed in this paper. the toxicity (ec50m) of 58 mixtures is determined using photobacterium phosphoreum. analysis of these ec50m and the mole fraction of the individual chemical fragments (br, cl, no2, oh, nh2) helps work out the fragment toxicity contribution (deltati) to ec50m. thus, a linear regression equation is established between the toxicity contribution deltati and the fragment constants of ... | 2004 | 15025158 |
| two cases of fatal necrotizing fasciitis caused by photobacterium damsela in japan. | we encountered two cases of fatal necrotizing fasciitis caused by photobacterium damsela in japan. both cases occurred in fishermen who became sick after fishing. they developed multiple organ failure within 20 to 36 h from the onset of initial symptoms despite intensive chemotherapy and surgical treatments. | 2004 | 15004123 |
| the effect of in vivo growth on the cellular and extracellular components of the marine bacterial pathogen photobacterium damsela subsp. piscicida. | photobacterium damsela subsp. piscicida, the causative agent of fish pasteurellosis, was grown in vivo. bacterial cells and extracellular products (ecps) were analysed via electrophoresis and immunoblot analysis, using specific sea bass antisera. growth in vivo induced the synthesis of unique bacterial cell proteins at > 206, 206, 21.3, 18, 7.6 and < 7.6 kda. sea bass serum raised against live bacterial cells of the pathogen and especially a sea bass serum raised against formalin-inactivated bac ... | 2004 | 14986934 |
| characterization of psychrotrophic bacteria in the surface and deep-sea waters from the northwestern pacific ocean based on 16s ribosomal dna analysis. | seventy-eight 4 degrees c-culturable bacteria were isolated using zobell 2216e medium from surface (0-200 m) and deep-sea (1000-9671 m) waters in the northwestern pacific ocean. growth studies indicated that all 4 degrees c-culturable bacteria were psychrotrophs. six phylotypes were observed in the surface water samples and 8 phylotypes in the deep-sea waters. phylogenetic characterization based on 16s ribosomal dna sequence analysis of the representative phylotypes revealed that some bacterial ... | 2001 | 14961338 |
| hoarding behavior in the golden hamster (mesocricetus auratus auratus). | 1950 | 14803683 | |
| the influence of penicillin on large body production by luminous bacteria. | 1950 | 14774347 | |
| genomic polymorphism in symbiotic populations of photobacterium leiognathi. | photobacterium leiognathi forms a bioluminescent symbiosis with leiognathid fishes, colonizing the internal light organ of the fish and providing its host with light used in bioluminescence displays. strains symbiotic with different species of the fish exhibit substantial phenotypic differences in symbiosis and in culture, including differences in 2-d page protein patterns and profiles of indigenous plasmids. to determine if such differences might reflect a genetically based symbiont-strain/host ... | 2004 | 14756879 |
| collaborative effects of photobacterium cuzn superoxide dismutase (sods) and human ap endonuclease in dna repair and sod-deficient escherichia coli under oxidative stress. | the defenses against free radical damage include specialized repair enzymes that correct oxidative damage in dna and detoxification systems such as superoxide dismutases (sods). these defenses may be coordinated genetically as global responses. we hypothesized that the expression of sod and dna repair genes would inhibit dna damage under oxidative stress. therefore, protection of escherichia coli mutants deficient in sod and dna repair genes (sod-, xth-, and nfo-) was demonstrated by transformin ... | 2004 | 14744629 |
| interactions of soil-derived dissolved organic matter with phenol in peroxidase-catalyzed oxidative coupling reactions. | the influence of dissolved soil organic matter (dsom) derived from three geosorbents of different chemical composition and diagenetic history on the horseradish peroxidase (hrp) catalyzed oxidative coupling reactions of phenol was investigated. phenol conversion and precipitate-product formation were measured, respectively, by hplc and radiolabeled species analysis. fourier transform infrared (ftir) spectroscopy and capillary electrophoresis (ce) were used to characterize the products of enzymat ... | 2004 | 14740756 |
| evolution of toxicity upon wet catalytic oxidation of phenol. | this work reports on the evolution of the toxicity of phenol-containing simulated wastewater upon catalytic wet oxidation with a commercial copper-based catalyst (engelhard cu-0203t). the results of the study show that this catalyst enhances detoxification, in addition to its effect on the oxidation rate. the ec50 values of the intermediates identified throughout the oxidation route of phenol have been determined and used to predict the evolution of toxicity upon oxidation. the predicted values ... | 2004 | 14740728 |
| construction of a safe, stable, efficacious vaccine against photobacterium damselae ssp. piscicida. | vaccination with bacterial auxotrophs, particularly those with an interruption in the common pathway of aromatic amino-acid biosynthesis, known as the shikimate pathway, has been shown to be effective in the prevention of a variety of bacterial diseases. in order to evaluate this approach to vaccine development in the important marine pathogen photobacterium damselae subsp. piscicida, the aroa gene of the shikimate pathway was identified from a p. damselae subsp. piscicida genomic library by com ... | 2003 | 14735921 |
| [antimicrobial activity of saponins from hedera taurica c a r r]. | the antibacterial and antifungal action of saponins sx1, taurozide h2, taurozide i and their effect on bioluminescent system of photobacteria photobacterium phosphoreum (cohn) ford were investigated. saponins h2 and i had no antimicrobial effect. saponin sx1 possessed antifungal activity in vitro with respect to candida albicans, c. krusei, c. tropicalis. saponin sx1 is less toxic to p. phosphoreum than well-known antiseptics--chlorhexedin and miramistin. | 2003 | 14723156 |
| rapidly advancing necrotizing fasciitis caused by photobacterium (vibrio) damsela: a hyperaggressive variant. | to describe the first case of vibrio damsela necrotizing fasciitis in new england, emphasizing the importance of very early operative intervention to achieve source control in this extremely aggressive infection. | 2004 | 14707592 |
| modelling and predicting the simultaneous growth of listeria monocytogenes and spoilage micro-organisms in cold-smoked salmon. | to evaluate and model the simultaneous growth of listeria monocytogenes and spoilage micro-organisms in cold-smoked salmon. | 2004 | 14678163 |
| mechanism of concentration addition toxicity: they are different for nonpolar narcotic chemicals, polar narcotic chemicals and reactive chemicals. | according to the toxicity mechanism of the individual chemicals, the concentration addition toxicity mechanism is revealed for nonpolar-narcotic-chemical mixtures, polar-narcotic-chemical mixtures and reactive-chemical mixtures, respectively. for nonpolar-narcotic-chemical mixtures, the partitioning of individual chemicals from water to biophase was determined, and the result shows that their concentration additive effect results from no competitive partitioning among individual chemicals. for p ... | 2004 | 14675847 |
| immune response of gilt-head seabream (sparus aurata) to antigens from photobacterium damselae subsp. piscicida. | 2004 | 14675834 | |
| accuracy of six commercially available systems for identification of members of the family vibrionaceae. | six commercially available bacterial identification products were tested with vibrio alginolyticus (12 strains), v. cholerae (30 strains), photobacterium (vibrio) damselae (10 strains), v. fluvialis (10 strains), v. furnissii (4 strains), v. hollisae (10 strains), v. metschnikovii (9 strains), v. mimicus (10 strains), v. parahaemolyticus (30 strains), and v. vulnificus (10 strains) to determine the accuracy of each system for identification. the products included api 20e, crystal e/nf, microscan ... | 2003 | 14662957 |
| simple and rapid detection of photobacterium damselae ssp. piscicida by a pcr technique and plating method. | to detect photobacterium damselae ssp. piscicida using the pcr technique and plating method. | 2003 | 14633013 |
| oxidative stress monitoring in biological samples by bioluminescent method. | the integral bioluminescent biotest with lyophilized fluorescent bacteria was used for monitoring of lpo processes in tissue extracts and serum of rats exposed to stress. a relationship between the content of mda (lpo indicator) and fluorescence of bacteria was observed in all biological samples. | 2003 | 14631513 |
| nutritional composition and microflora of the fresh and fermented skate (raja kenojei) skins. | the proximate compositions of fresh and fermented skate skin were each 75.95% and 74.5% moisture, 22.7% and 21.8% protein, 0.5% and 0.7% lipid and 0.6% and 0.9% ash, respectively. the predominant minerals were potassium and phosphorus (i.e. 53.5 and 33.0 mg/100 g in fresh skin, and 10.46 and 10.51 mg/100 g in fermented skin, respectively). amino acid concentrations were lower in the fermented skin compared with the fresh skin. histidine, glycine, alanine and glutamic acid were the major free ami ... | 2004 | 14630591 |
| virulence of photobacterium damselae subsp. piscicida in cultured cobia rachycentron canadum. | an outbreak of serious mortality among the cultured cobia rachycentron canadum (weighing 3 kg) characterized by the presence of whitish granulomatous deposits on the kidney, liver and spleen occurred in july of 2000 in taiwan. a non-motile strain cp1 was isolated from kidney and/or liver on tryptic soy agar and/or brain heart infusion agar plates (both supplemented with 1% nacl, w/v). this strain was characterized and identified as photobacterium damselae subsp. piscicida using biochemical chara ... | 2003 | 14625900 |
| isolation and identification of photobacterium phosphoreum from an unexpected niche: migrating salmon. | six luminous bacteria were isolated from migrating salmon in the yukon river, alaska. all isolates were identified as photobacterium phosphoreum. previous studies suggest that p. phosphoreum is an exclusively marine bacterium, while our alaskan isolates are from salmon which migrated up to 1,228 km from the marine environment. | 2003 | 14602659 |
| bacterial influences on atlantic halibut hippoglossus hippoglossus yolk-sac larval survival and start-feed response. | a bacteria-free halibut larval rearing system was used to test 20 bacterial isolates, from british halibut hatcheries, for their toxicity towards halibut yolk-sac larvae under microbially controlled conditions. the isolates tested spanned a range of genera and species (pseudoalteromonas, halomonas marina, vibrio salmonicida-like, photobacterium phosphoreum and v. splendidus species). a pathogen of turbot, scophthalmus maximus, v. anguillarum 91079, and 2 isolates from adult halibut were also inc ... | 2003 | 14598986 |
| [effect of mycotoxin t-2 on bioluminescence intensity of bacteria]. | the acute and chronic toxicity of t-2 was studied by bioluminescent method with the use of two strains of luminous bacteria--p. phosphorum sq3 u v. fischeri f1 as biological objects. it was shown that in acute experiments after 10 min incubation of bacteria in the presence of t-2 the bioluminescence inhibition on the 50% level was observed at the toxin concentration equal to 12 mg/ml. in chronic experiments such a level of bioluminescence inhibition was registered after 16 hours incubation at th ... | 2003 | 14577159 |
| development of qsars for predicting the joint effects between cyanogenic toxicants and aldehydes. | quantitative structure-activity relationship (qsar) approaches are proposed in this study to predict the joint effects of mixture toxicity. the initial investigation studies the joint effects between cyanogenic toxicants and aldehydes to photobacterium phosphoreum. joint effects are found to result from the formation of a carbanion intermediate produced through the chemical interactions between cyanogenic toxicants and aldehydes. further research indicates that the formation of carbanion interme ... | 2003 | 14565777 |
| [comparative study of microbial communities from cultured and natural population of the mussel mytilus trossulus in peter the great bay]. | the 525 strains of heterotrophic bacteria isolated from natural and cultured populations of the mussel mytilus trossulus and the surrounding seawater were identified to a genus level on the basis of phenotypic analysis and the fatty acid composition of cell lipids. gram-negative isolates were dominated by six genera of the family enterobacteriaceae and by the genera pseudoalteromonas, vibrio, photobacterium, cytophaga/flavobacterium/bacteroides, pseudomonas, and moraxella, gram-positive isolates ... | 2003 | 14526545 |
| purification and characterization of an antibacterial protein in the skin secretion of rockfish sebastes schlegeli. | an antibacterial protein in the skin secretion of rockfish (sebastes schlegeli) was purified by lectin affinity chromatography on con a-sepharose and gel filtration on tskgel g3000sw. the antibacterial protein featured the high molecular mass and selective action against gram-negative bacteria. the molecular mass of the protein was estimated to be approximately 150 kda in gel filtration and approximately 75 kda by sds-page, suggesting that it is dimeric. the antibacterial principle was an acidic ... | 2003 | 14522599 |
| three-dimensional quantitative structure-activity relationship study for phenylsulfonyl carboxylates using comfa and comsia. | from both the comparative molecular field analysis (comfa) and the comparative molecular similarity indices analysis (comsia), the paper describes two three-dimensional quantitative structure-activity relationship (3d-qsar) models for the acute toxicity logec50 (15 min-ec50 in micromoll(-1)) of 56 phenylsulfonyl carboxylates on photobacterium phosphoreum. two models yield the leave-one-out cross-validated correlation coefficient q2 values of 0.823 and 0.713, and the conventional correlation coef ... | 2003 | 14505717 |
| [studies on cultivation of photobacterium phosphoreum. iii. on a measurement of light intensity and some applications]. | 1962 | 14458106 |