Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| improved biomass and protein production in solid-state cultures of an aspergillus sojae strain harboring the vitreoscilla hemoglobin. | the biotechnological value of aspergillus sojae atcc 20235 (a. sojae) for production of pectinases in solid-state fermentation (ssf) has been demonstrated recently. however, a common drawback of fungal solid-state cultures is the poor diffusion of oxygen into the fungi that limits its growth and biological productivity. the bacterial vitreoscilla hemoglobin (vhb) has favored the metabolism and productivities of various bacterial and yeast strains besides alleviating hypoxic conditions of its nat ... | 2015 | 26224427 |
| development of a new pcambia binary vector using gateway® technology. | pcambia vectors have become popular for their easy handling, stability and the existence of a range of selection and reporter genes. however, these vectors have yet to integrate the gateway® cloning system, which has enabled site-specific recombination without the need for restriction enzymes and ligases. this paper sets out to convert the pcambia2300 binary vector into a destination vector with the gateway® cassette driven by the camv35s promoter. the destination vector, pcamway35s, was then ev ... | 2015 | 26210260 |
| development of a transformation system for hirsutella spp. and visualization of the mode of nematode infection by gfp-labeled h. minnesotensis. | hirsutella rhossiliensis and h. minnesotensis are endoparasitic fungi of the second-stage juvenile (j2) of the soybean cyst nematode (heterodera glycines) in nature. they also parasitize both h. glycines j2 and caenorhabditis elegans on agar plates. agrobacterium tumefaciens-mediated transformation conditions were established for these hirsutella spp. the resulting transformants were similar to the corresponding wild-type strains. the infection processes of h. glycines j2 and c. elegans second l ... | 2015 | 26190283 |
| transgene expression in tick cells using agrobacterium tumefaciens. | ticks transmit infectious agents to humans and other animals. genetic manipulation of vectors like ticks could enhance the development of alternative disease control strategies. transgene expression using the phytopathogen agrobacterium tumefaciens has been shown to promote the genetic modification of non-plant cells. in the present work we developed t-dna constructs for a. tumefaciens to mediate transgene expression in hela cells as well as rhipicephalus microplus tick cells. translational fusi ... | 2015 | 26188857 |
| antibody proteolysis: a common picture emerging from plants. | we have recently characterized the degradation profiles of 2 human igg1 monoclonal antibodies, the tumor-targeting mab h10 and the anti-hiv mab 2g12. both mabs were produced in plants either as stable transgenics or using a transient expression system based on leaf agroinfiltration. the purified antibodies were separated by 1de and protein bands were characterized by n-terminal sequencing. the proteolytic cleavage sites identified in the heavy chain (hc) of both antibodies were localized in 3 in ... | 2015 | 26186119 |
| production of d-psicose from d-fructose by whole recombinant cells with high-level expression of d-psicose 3-epimerase from agrobacterium tumefaciens. | the specific activity of recombinant escherichia coli cells expressing the double-site variant (i33l-s213c) d-psicose 3-epimerase (dpease) from agrobacterium tumefaciens was highest at 24 h of cultivation time in terrific broth (tb) medium among the media tested. the contents of crude protein and dpease in recombinant cells at 24 h were 37.0 and 8.6% (w/w), respectively, indicating that the enzyme was highly expressed. the reaction conditions for the production of d-psicose from d-fructose by wh ... | 2016 | 26183861 |
| a novel phenolic compound, chloroxynil, improves agrobacterium-mediated transient transformation in lotus japonicus. | agrobacterium-mediated transformation is a commonly used method for plant genetic engineering. however, the limitations of agrobacterium host-plant interactions and the complexity of plant tissue culture often make the production of transgenic plants difficult. transformation efficiency in many legume species, including soybean and the common bean, has been reported to be quite low. to improve the transformation procedure in legumes, we screened for chemicals that increase the transformation eff ... | 2015 | 26176780 |
| heterologous dna uptake in cultured symbiodinium spp. aided by agrobacterium tumefaciens. | plant-targeted pcb302 plasmids containing sequences encoding gfp fusions with a microtubule-binding domain; gfp with the fimbrin actin-binding domain 2; and gfp with atrack1c from arabidopsis thaliana, all harbored in agrobacterium tumefaciens, were used to assay heterologous expression on three different clades of the photosynthetic dinoflagellate, symbiodinium. accessibility to the resistant cell wall and through the plasma membrane of these dinoflagellates was gained after brief but vigorous ... | 2015 | 26167858 |
| agrobacterium-mediated in planta genetic transformation of sugarcane setts. | an efficient, reproducible, and genotype-independent in planta transformation has been developed for sugarcane using setts as explant. traditional agrobacterium-mediated genetic transformation and in vitro regeneration of sugarcane is a complex and time-consuming process. development of an efficient agrobacterium-mediated transformation protocol, which can produce a large number of transgenic plants in short duration is advantageous. hence, in the present investigation, we developed a tissue cul ... | 2015 | 26152769 |
| agrobacterium tumefaciens gene transfer: how a plant pathogen hacks the nuclei of plant and nonplant organisms. | agrobacterium species are soilborne gram-negative bacteria exhibiting predominantly a saprophytic lifestyle. only a few of these species are capable of parasitic growth on plants, causing either hairy root or crown gall diseases. the core of the infection strategy of pathogenic agrobacteria is a genetic transformation of the host cell, via stable integration into the host genome of a dna fragment called t-dna. this genetic transformation results in oncogenic reprogramming of the host to the bene ... | 2015 | 26151736 |
| a pterin-dependent signaling pathway regulates a dual-function diguanylate cyclase-phosphodiesterase controlling surface attachment in agrobacterium tumefaciens. | the motile-to-sessile transition is an important lifestyle switch in diverse bacteria and is often regulated by the intracellular second messenger cyclic diguanylate monophosphate (c-di-gmp). in general, high c-di-gmp concentrations promote attachment to surfaces, whereas cells with low levels of signal remain motile. in the plant pathogen agrobacterium tumefaciens, c-di-gmp controls attachment and biofilm formation via regulation of a unipolar polysaccharide (upp) adhesin. the levels of c-di-gm ... | 2015 | 26126849 |
| improving plant transformation using agrobacterium tumefaciens. | here, we report a quick and low-cost method to improve plant transformation using agrobacterium tumefaciens. this method involves the use of physical wounding, ultrasound, and an increase in exposure time to the bacteria. we show how the transformation rate increased from 0 to 14% when an ultrasound pulse of 10 s was used in conjunction with 96 h of bacterial exposure in eclipta alba explants. | 2015 | 26125878 |
| mild and severe cereal yellow dwarf viruses differ in silencing suppressor efficiency of the p0 protein. | viral pathogenicity has often been correlated to the expression of the viral encoded-rna silencing suppressor protein (ssp). the silencing suppressor activity of the p0 protein encoded by cereal yellow dwarf virus-rpv (cydv-rpv) and -rps (cydv-rps), two poleroviruses differing in their symptomatology was investigated. cydv-rpv displays milder symptoms in oat and wheat whereas cydv-rps is responsible for more severe disease. we showed that both p0 proteins (p0(cy-rpv) and p0(cy-rps)) were able to ... | 2015 | 26116275 |
| rapid mapping of insertional mutations to probe cell wall regulation in cryptococcus neoformans. | random insertional mutagenesis screens are important tools in microbial genetics studies. investigators in fungal systems have used the plant pathogen agrobacterium tumefaciens to create tagged, random mutations for genetic screens in their fungal species of interest through a unique process of trans-kingdom cellular transconjugation. however, identifying the locations of insertion has traditionally required tedious pcr-based methods, limiting the effective throughput of this system. we have dev ... | 2015 | 26112692 |
| corrigendum: induction, suppression and requirement of rna silencing pathways in virulent agrobacterium tumefaciens infections. | 2015 | 26111511 | |
| production of conjugated linoleic acid by heterologous expression of linoleic acid isomerase in oleaginous fungus mortierella alpina. | to increase the commercial value of oleaginous fungus mortierella alpina by incorporation of trans-10,cis-12 conjugated linoleic acid (cla) into the polyunsaturated fatty acids (pufas) of m. alpina via propionibacterium acnes isomerase (pai) conversion. | 2015 | 26109146 |
| cell-cell communication in yeast using auxin biosynthesis and auxin responsive crispr transcription factors. | an engineering framework for synthetic multicellular systems requires a programmable means of cell-cell communication. such a communication system would enable complex behaviors, such as pattern formation, division of labor in synthetic microbial communities, and improved modularity in synthetic circuits. however, it remains challenging to build synthetic cellular communication systems in eukaryotes due to a lack of molecular modules that are orthogonal to the host machinery, easy to reconfigure ... | 2016 | 26102245 |
| a suite of gateway® compatible ternary expression vectors for functional analysis in zymoseptoria tritici. | gene overexpression is a widely used functional genomics approach in fungal biology. however, to date it has not been established in zymoseptoria tritici which is an important pathogen of wheat (triticum species). here we report a suite of gateway® recombination compatible ternary expression vectors for agrobacterium tumefaciens mediated transformation of z. tritici. the suite of 32 vectors is based on a combination of four resistance markers for positive selection against glufosinate ammonium, ... | 2015 | 26092805 |
| exploitation of sulfonylurea resistance marker and non-homologous end joining mutants for functional analysis in zymoseptoria tritici. | the lack of techniques for rapid assembly of gene deletion vectors, paucity of selectable marker genes available for genetic manipulation and low frequency of homologous recombination are major constraints in construction of gene deletion mutants in zymoseptoria tritici. to address these issues, we have constructed ternary vectors for agrobacterium tumefaciens mediated transformation of z. tritici, which enable the single step assembly of multiple fragments via yeast recombinational cloning. the ... | 2015 | 26092796 |
| virus induced gene silencing (vigs) for functional analysis of wheat genes involved in zymoseptoria tritici susceptibility and resistance. | virus-induced gene silencing (vigs) has emerged as a powerful reverse genetic technology in plants supplementary to stable transgenic rnai and, in certain species, as a viable alternative approach for gene functional analysis. the rna virus barley stripe mosaic virus (bsmv) was developed as a vigs vector in the early 2000s and since then it has been used to study the function of wheat genes. several variants of bsmv vectors are available, with some requiring in vitro transcription of infectious ... | 2015 | 26092793 |
| proline over-accumulation alleviates salt stress and protects photosynthetic and antioxidant enzyme activities in transgenic sorghum [sorghum bicolor (l.) moench]. | shoot-tip derived callus cultures of sorghum bicolor were transformed by agrobacterium tumefaciens as well as by bombardment methods with the mutated pyrroline-5-carboxylate synthetase (p5csf129a) gene encoding the key enzyme for proline biosynthesis from glutamate. the transgenics were selfed for three generations and t4 plants were examined for 100 mm nacl stress tolerance in pot conditions. the effect of salt stress on chlorophyll and carotenoid contents, photosynthetic rate, stomatal conduct ... | 2015 | 26065619 |
| bioconversion of d-glucose to d-psicose with immobilized d-xylose isomerase and d-psicose 3-epimerase on saccharomyces cerevisiae spores. | saccharomyces cerevisiae spores are dormant cells, which can tolerate various types of environmental stress. in our previous work, we successfully developed biological and chemical methods for enzyme immobilization based on the structures of s. cerevisiae spore wall. in this study, we employed biological and chemical approaches for the immobilization of d-xylose isomerase (xi) from thermus thermophilus and d-psicose 3-epimerase (dpease) from agrobacterium tumefaciens with yeast spores, respectiv ... | 2015 | 26065389 |
| mapping of the interaction between agrobacterium tumefaciens and vanda kasem's delight orchid protocorm-like bodies. | physical contact between a. tumefaciens and the target plant cell walls is essential to transfer and integrate the transgene to introduce a novel trait. chemotaxis response and attachment of agrobacterium towards vanda kasem's delight (vkd) protocorm-like bodies (plbs) were studied to analyse the interaction between agrobacterium and plb during the transformation event. the study shows that initially a. tumefaciens reversibly attached to plb surface via polar and lateral mode of adherence follow ... | 2015 | 26063938 |
| characterization of two-step deglycosylation via oxidation by glycoside oxidoreductase and defining their subfamily. | herein, we report a two-step deglycosylation mediated by the oxidation of glycoside which is different from traditional glycoside hydrolase (gh) mechanism. previously, we reported a novel flavin adenine dinucleotide (fad)-dependent glycoside oxidoreductase (fad-go) having deglycosylation activity. various features of the reaction of fad-go such as including mechanism and catalytic residue and substrate specificity were studied. in addition, classification of novel fad-go subfamily was attempted. ... | 2015 | 26057169 |
| development of an agrobacterium-mediated transformation system for the cold-adapted fungi pseudogymnoascus destructans and p. pannorum. | the mechanisms of cold adaptation by fungi remain unknown. this topic is of high interest due to the emergence of white-nose syndrome (wns), a skin infection of hibernating bats caused by pseudogymnoascus destructans (pd). recent studies indicated that apart from pd, there is an abundance of other pseudogymnoascus species in the hibernacula soil. we developed an agrobacterium tumefaciens-mediated transformation (atmt) system for pd and a related fungus pseudogymnoascus pannorum (pp) to advance e ... | 2015 | 26051491 |
| rhf1 gene is involved in the fruiting body production of cordyceps militaris fungus. | cordyceps militaris is an important medicinal fungus. commercialization of this fungus needs to improve the fruiting body production by molecular engineering. an improved agrobacterium tumefaciens-mediated transformation (atmt) method was used to select an insertional mutant (g38) which exhibited fast stromatal differentiation and increased yield. the rhf1 gene encoding filamentation protein was destroyed by a single t-dna and no rhf1 transcription was detected in mutant g38. to verify the funct ... | 2015 | 26047996 |
| surface plasmon resonance imaging reveals multiple binding modes of agrobacterium transformation mediator vire2 to ssdna. | vire2 is the major secreted protein of agrobacterium tumefaciens in its genetic transformation of plant hosts. it is co-expressed with a small acidic chaperone vire1, which prevents vire2 oligomerization. after secretion into the host cell, vire2 serves functions similar to a viral capsid in protecting the single-stranded transferred dna en route to the nucleus. binding of vire2 to ssdna is strongly cooperative and depends moreover on protein-protein interactions. in order to isolate the protein ... | 2015 | 26044711 |
| complete genome sequence of agrobacterium tumefaciens ach5. | agrobacterium tumefaciens is a phytopathogenic bacterium that causes crown gall disease. the strain ach5 was isolated from yarrow (achillea ptarmica l.) and is the wild-type progenitor of other derived strains widely used for plant transformation. here, we report the complete genome sequence of this bacterium. | 2015 | 26044425 |
| back from the dead; the curious tale of the predatory cyanobacterium vampirovibrio chlorellavorus. | an uncultured non-photosynthetic basal lineage of the cyanobacteria, the melainabacteria, was recently characterised by metagenomic analyses of aphotic environmental samples. however, a predatory bacterium, vampirovibrio chlorellavorus, originally described in 1972 appears to be the first cultured representative of the melainabacteria based on a 16s rrna sequence recovered from a lyophilised co-culture of the organism. here, we sequenced the genome of v. chlorellavorus directly from 36 year-old ... | 2015 | 26038723 |
| paracoccus denitrificans possesses two bior homologs having a role in regulation of biotin metabolism. | recently, we determined that bior, the gntr family of transcription factor, acts as a repressor for biotin metabolism exclusively distributed in certain species of α-proteobacteria, including the zoonotic agent brucella melitensis and the plant pathogen agrobacterium tumefaciens. however, the scenario is unusual in paracoccus denitrificans, another closely related member of the same phylum α-proteobacteria featuring with denitrification. not only does it encode two bior homologs pden_1431 and pd ... | 2015 | 26037461 |
| agrobacterium-mediated transient maft expression in mulberry (morus alba l.) leaves. | to optimize agrobacterium-mediated transient transformation assay in mulberry (morus alba l.), various infiltration methods, agrobacterium tumefaciens (a. tumefaciens) strains, and bacterial concentrations were tested in mulberry seedlings. compared with lba4404, gv3101 harboring pbe2133 plasmids presented stronger gus signals at 3 days post infiltration using syringe. recombinant plasmids pbe2133:gfp and pbe2133:gfp:maft were successfully constructed. transient expression of maft:gfp protein wa ... | 2015 | 26024368 |
| agrobacterium tumefaciens-mediated transformation of corn (zea mays l.) multiple shoots. | an agrobacterium tumefaciens-mediated corn transformation method based on multiple shoot tissue cultures was developed, which is effective with a variety of corn inbred lines and standard binary vectors. six factors that affected the success of corn transformation were tested, including a. tumefaciens strain, corn genotype, tissue culture growth stage, medium composition, co-culture temperature and surfactant treatment. agropine-type bacteria (eha 101 and agl 1) were eightfold more effective tha ... | 2014 | 26019506 |
| development of genetic tools for myceliophthora thermophila. | the thermophilic filamentous fungus myceliophthora thermophila has many suitable characteristics for industrial biotechnology and could be a promising new chassis system for synthetic biology, particularly the atcc 42464 strain, whose genome was sequenced in 2011. however, metabolic engineering of this strain using genetic approaches has not been reported owing to a lack of genetic tools for this organism. | 2015 | 26013561 |
| the agrobacterium tumefaciens ti plasmid virulence gene vire2 reduces sri lankan cassava mosaic virus infection in transgenic nicotiana benthamiana plants. | cassava mosaic disease is a major constraint to cassava cultivation worldwide. in india, the disease is caused by indian cassava mosaic virus (icmv) and sri lankan cassava mosaic virus (slcmv). the agrobacterium ti plasmid virulence gene vire2, encoding a nuclear-localized, single-stranded dna binding protein, was introduced into nicotiana benthamiana to develop tolerance against slcmv. leaf discs of transgenic n. benthamiana plants, harboring the vire2 gene, complemented a vire2 mutation in a. ... | 2015 | 26008704 |
| antimicrobial, antitumor and brine shrimp lethality assay of ranunculus arvensis l. extracts. | to investigate the antitumor activity, brine shrimp lethality assay, antibacterial and antifungal activity of methanol extract (me), water extract (we), acetone extract (ae), chloroform extract (ce), methanol-water extract (mwe), methanol-acetone extract (mae), methanol-chloroform extract (mce) of ranunculus arvensis (l.). antitumor activity was evaluated with agrobacterium tumefaciens (at10) induced potato disc assay. cytotoxicity was evaluated with brine shrimp lethality assay. antibacterial a ... | 2015 | 26004705 |
| controlling the interplay between agrobacterium tumefaciens and plants during the transient expression of proteins. | in may 2012, the first plant-derived biopharmaceutical protein received full regulatory approval for therapeutic use in humans. although plant-based expression systems have many advantages, they can suffer from low expression levels and, depending on the species, the presence of potentially toxic secondary metabolites. transient expression mediated by agrobacterium tumefaciens can be used to increase product yields but may also increase the concentration of secondary metabolites generated by pla ... | 2015 | 25997443 |
| a genetic screen for pathogenicity genes in the hemibiotrophic fungus colletotrichum higginsianum identifies the plasma membrane proton pump pma2 required for host penetration. | we used insertional mutagenesis by agrobacterium tumefaciens mediated transformation (atmt) to isolate pathogenicity mutants of colletotrichum higginsianum. from a collection of 7200 insertion mutants we isolated 75 mutants with reduced symptoms. 19 of these were affected in host penetration, while 17 were affected in later stages of infection, like switching to necrotrophic growth. for 16 mutants the location of t-dna insertions could be identified by pcr. a potential plasma membrane h(+)-atpas ... | 2015 | 25992547 |
| co-expression of hepatitis c virus polytope-hbsag and p19-silencing suppressor protein in tobacco leaves. | plants transformed by virus-based vectors have emerged as promising tools to rapidly express large amounts and inexpensive antigens in transient condition. | 2016 | 25990925 |
| optimization of in vitro regeneration and agrobacterium tumefaciens-mediated transformation with heat-resistant cdna in brassica oleracea subsp. italica cv. green marvel. | an efficient system for shoot regeneration and agrobacterium tumefaciens-mediated transformation of brassica oleracea cv. green marvel cultivar is described. this study focuses on developing shoot regeneration from hypocotyl explants of broccoli cv. green marvel using thidiazuron (tdz), zeatin, and kinetin, the optimization of factors affecting agrobacterium-mediated transformation of the hypocotyl explants with heat-resistant cdna, followed by the confirmation of transgenicity of the regenerant ... | 2015 | 25986972 |
| comparative performance of modified full-length and truncated bacillus thuringiensis-cry1ac genes in transgenic tomato. | bt-cry1ac gene has been reputedly effective against helicoverpa armigera a notorious lepidopteran pest. reports on the expression of full-length and truncated cry1ac genes in plants for effective resistance against helicoverpa sp. have been documented however, their performance is still ambiguous. moreover, the question remains to be addressed that truncation of 3' end of the native gene was documented and suggested for active insecticidal toxin production while the most successful transgenic ev ... | 2015 | 25977893 |
| better rooting procedure to enhance survival rate of field grown malaysian eksotika papaya transformed with 1-aminocyclopropane-1-carboxylic acid oxidase gene. | a high survival rate for transformed papaya plants when transferred to the field is useful in the quest for improving the commercial quality traits. we report in this paper an improved rooting method for the production of transformed malaysian eksotika papaya with high survival rate when transferred to the field. shoots were regenerated from embryogenic calli transformed with antisense and rnai constructs of 1-aminocyclopropane-1-carboxylic acid oxidase (aco) genes using the agrobacterium tumefa ... | 2013 | 25969786 |
| [opine biosynthesis and catabolism genes of agrobacterium tumefaciens and agrobacterium rhizogenes]. | agrobacterium is a genus of soil bacteria with the ability to transform plant cells by a t-dna-sequence located on the pti/pri- plasmid containing a set of genes expressed in plant cells. expression of these genes leads to a proliferation of transformed cells, with the subsequent formation of tumors or growths of roots and the synthesis of opines--products of the condensation of amino acids with ketoacids or sugars used by agrobacteria as a source of carbon and nitrogen. in this review, we syste ... | 2015 | 25966579 |
| optimization of translation profiles enhances protein expression and solubility. | mrna is translated with a non-uniform speed that actively coordinates co-translational folding of protein domains. using structure-based homology we identified the structural domains in epoxide hydrolases (ehs) and introduced slow-translating codons to delineate the translation of single domains. these changes in translation speed dramatically improved the solubility of two ehs of metagenomic origin in escherichia coli. conversely, the importance of transient attenuation for the folding, and con ... | 2015 | 25965266 |
| efficient shoots regeneration and genetic transformation of bacopa monniera. | bacopa monniera is an important source of metabolites with pharmaceutical value. it has been regarded as a valuable medicinal plant and its entire commercial requirement is met from wild natural population. recently, metabolic engineering has emerged as an important solution for sustained supply of assured and quality raw material for the production of active principles. present report describes efficient in vitro multiplication and transformation method for genetic manipulation of this species. ... | 2015 | 25964718 |
| expression of enterovirus 71 virus-like particles in transgenic enoki (flammulina velutipes). | no commercial vaccines are currently available for enterovirus 71 (ev71) infection. oral virus-like particle (vlp) vaccines are regarded as a better choice for prevention from food-borne diseases compared with injected whole virus vaccines. unfortunately, the application of oral vlp vaccines produced from transgenic plants was limited due to the concerns of gene contamination. alternatively, using transgenic mushrooms retains the advantages of transgenic plants and tremendously reduce risks of g ... | 2015 | 25957149 |
| diversity and n-acyl-homoserine lactone production by gammaproteobacteria associated with avicennia marina rhizosphere of south indian mangroves. | the diversity of n-acyl-homoserine lactone (ahl)-producing rhizosphere bacterial community associated with avicennia marina in the mangrove ecosystems of south india was investigated. approximately 800 rhizobacteria were isolated from a. marina, and they were screened for the production of ahl using two biosensors, chromobacterium violaceum cv026 and agrobacterium tumefaciens ntl4 (pzlr4). among the total isolates screened, 7% of the rhizobacteria showed positive induction for ahl signals. the b ... | 2015 | 25956585 |
| gene overexpression and rna silencing tools for the genetic manipulation of the s-(+)-abscisic acid producing ascomycete botrytis cinerea. | the phytopathogenic ascomycete botrytis cinerea produces several secondary metabolites that have biotechnical significance and has been particularly used for s-(+)-abscisic acid production at the industrial scale. to manipulate the expression levels of specific secondary metabolite biosynthetic genes of b. cinerea with agrobacterium tumefaciens-mediated transformation system, two expression vectors (pcbh1 and pcbg1 with different selection markers) and one rna silencing vector, pcbsilent1, were ... | 2015 | 25955649 |
| characterization and comparative analysis of promoters from three plant pararetroviruses associated with dahlia (dahlia variabilis). | two distinct caulimoviruses, dahlia mosaic virus (dmv) and dahlia common mosaic virus (dcmv), and an endogenous plant pararetroviral sequence (dveprs, formerly known as dmv-d10) were reported from dahlia (dahlia spp). promoter elements from these dahlia-associated pararetroviruses were identified and characterized. the tata box, the caat box, the transcription start site, the polyadenylation signal, and regulation factors, characteristic of caulimovirus promoters, were present in each of these p ... | 2015 | 25947569 |
| transgenic alfalfa plants expressing the sweetpotato orange gene exhibit enhanced abiotic stress tolerance. | alfalfa (medicago sativa l.), a perennial forage crop with high nutritional content, is widely distributed in various environments worldwide. we recently demonstrated that the sweetpotato orange gene (ibor) is involved in increasing carotenoid accumulation and enhancing resistance to multiple abiotic stresses. in this study, in an effort to improve the nutritional quality and environmental stress tolerance of alfalfa, we transferred the ibor gene into alfalfa (cv. xinjiang daye) under the contro ... | 2015 | 25946429 |
| cyclic di-gmp acts as a cell cycle oscillator to drive chromosome replication. | fundamental to all living organisms is the capacity to coordinate cell division and cell differentiation to generate appropriate numbers of specialized cells. whereas eukaryotes use cyclins and cyclin-dependent kinases to balance division with cell fate decisions, equivalent regulatory systems have not been described in bacteria. moreover, the mechanisms used by bacteria to tune division in line with developmental programs are poorly understood. here we show that caulobacter crescentus, a bacter ... | 2015 | 25945741 |
| agrobacterium tumefaciens and interkingdom genetic exchange. | 1989 | 25945402 | |
| the all-alpha domains of coupling proteins from the agrobacterium tumefaciens virb/vird4 and enterococcus faecalis pcf10-encoded type iv secretion systems confer specificity to binding of cognate dna substrates. | bacterial type iv coupling proteins (t4cps) bind and mediate the delivery of dna substrates through associated type iv secretion systems (t4sss). t4cps consist of a transmembrane domain, a conserved nucleotide-binding domain (nbd), and a sequence-variable helical bundle called the all-alpha domain (aad). in the t4cp structural prototype, plasmid r388-encoded trwb, the nbd assembles as a homohexamer resembling reca and dna ring helicases, and the aad, which sits at the channel entrance of the hom ... | 2015 | 25939830 |
| enhancing t-dna transfer efficiency in barley (hordeum vulgare l.) cells using extracellular cellulose and lectin. | a major limitation of transforming barley tissues by agrobacterium tumefaciens is the low frequency of t-dna transfer due to recalcitrance of barley as a host. the effect of extracellular cellulose and lectin on agrobacterium transformation efficiency was investigated in this study. barley callus cultures were transformed with the agl1 strain containing the vector pbi121 in the presence of 10 mg ml(-1) cellulose or 0.001, 0.05 and 0.1 mg ml(-1) lectin. addition of cellulose significantly (p ≤ 0. ... | 2015 | 25935223 |
| genetic transformation of extremophilic fungi acidea extrema and acidothrix acidophila. | intact, growing cells of strongly acidophilic fungi acidea extrema and acidothrix acidophila have been successfully transformed by introduction of heterologous dna fragment (composed of the glyceraldehyde-phosphate-dehydrogenase gene promoter from emericella nidulans, a metallothionein-coding gene asmt1 from amanita strobiliformis and glyceraldehyde-phosphate-dehydrogenase gene terminator from colletotrichum gloeosporioides) with the length of 1690 bp. the transformation procedure was based on t ... | 2015 | 25934267 |
| overexpression of 3-deoxy-7-phosphoheptulonate synthase gene from gossypium hirsutum enhances arabidopsis resistance to verticillium wilt. | expression of dhs1 in cotton is induced upon infection by verticillium dahliae , and overexpression of ghdhs1 endows transgenic arabidopsis plants excellent verticillium resistance. verticillium wilt is caused by a soil-borne fungus verticillium dahliae. resistance in most cotton cultivars is either scarce or unavailable, making verticillium wilt a major obstacle in cotton production. here, we identified a 3-deoxy-7-phosphoheptulonate synthase (dhs, ec 4.1.2.15) gene from gossypium hirsutum, nam ... | 2015 | 25929795 |
| sonication, vacuum infiltration and thiol compounds enhance the agrobacterium-mediated transformation frequency of withania somnifera (l.) dunal. | in the present study, we have established a stable transformation protocol via agrobacterium tumafacines for the pharmaceutically important withania somnifera. six day-old nodal explants were used for 3 day co-cultivation with agrobacterium tumefaciens strain lba4404 harbouring the vector pcamiba2301. among the different injury treatments, sonication, vacuum infiltration and their combination treatments tested, a vacuum infiltration for 10 min followed by sonication for 10 sec with a. tumefacien ... | 2015 | 25927703 |
| root-associated bacterial endophytes from ralstonia solanacearum resistant and susceptible tomato cultivars and their pathogen antagonistic effects. | this study was undertaken to assess if the root-associated native bacterial endophytes in tomato have any bearing in governing the host resistance to the wilt pathogen ralstonia solanacearum. internal colonization of roots by bacterial endophytes was confirmed through confocal imaging after syto-9 staining. endophytes were isolated from surface-sterilized roots of 4-weeks-old seedlings of known wilt resistant (r) tomato cultivar arka abha and susceptible (s) cv. arka vikas on nutrient agar after ... | 2015 | 25926818 |
| new polyphenols from a deep sea spiromastix sp. fungus, and their antibacterial activities. | eleven new polyphenols namely spiromastols a-k (1-11) were isolated from the fermentation broth of a deep sea-derived fungus spiromastix sp. mccc 3a00308. their structures were determined by extensive nmr data and mass spectroscopic analysis in association with chemical conversion. the structures are classified as diphenyl ethers, diphenyl esters and isocoumarin derivatives, while the n-propyl group in the analogues is rarely found in natural products. compounds 1-3 exhibited potent inhibitory e ... | 2015 | 25913707 |
| proteomics and genetics for identification of a bacterial antimonite oxidase in agrobacterium tumefaciens. | antimony (sb) and its compounds are listed by the united states environmental protection agency (usepa, 1979) and the european union (cec, 1976) as a priority pollutant. microbial redox transformations are presumed to be an important part of antimony cycling in nature; however, regulation of these processes and the enzymology involved are unknown. in this study, comparative proteomics and reverse transcriptase-pcr analysis of sb(iii)-oxidizing bacterium agrobacterium tumefaciens gw4 revealed an ... | 2015 | 25909855 |
| the genome of cultivated sweet potato contains agrobacterium t-dnas with expressed genes: an example of a naturally transgenic food crop. | agrobacterium rhizogenes and agrobacterium tumefaciens are plant pathogenic bacteria capable of transferring dna fragments [transfer dna (t-dna)] bearing functional genes into the host plant genome. this naturally occurring mechanism has been adapted by plant biotechnologists to develop genetically modified crops that today are grown on more than 10% of the world's arable land, although their use can result in considerable controversy. while assembling small interfering rnas, or sirnas, of sweet ... | 2015 | 25902487 |
| mdc-analyzer-facilitated combinatorial strategy for improving the activity and stability of halohydrin dehalogenase from agrobacterium radiobacter ad1. | halohydrin dehalogenase from agrobacterium radiobacter ad1 (hhec) displays a broad substrate range with high regio- and enantioselectivity of both ring-closure and ring-opening reactions, making the enzyme a useful catalyst for the production of optically pure epoxides and β-substituted alcohols. in this study, we report a novel method using an mdc-analyzer-facilitated combinatorial strategy to improve the activity and stability of hhec by simultaneously randomizing multiple contiguous residues. ... | 2015 | 25896949 |
| identification of the genes involved in the fruiting body production and cordycepin formation of cordyceps militaris fungus. | a mutant library of cordyceps militaris was constructed by improved agrobacterium tumefaciens-mediated transformation and screened for degradation features. six mutants with altered characters in in vitro and in vivo fruiting body production, and cordycepin formation were found to contain a single copy t-dna. t-dna flanking sequences of these mutants were identified by thermal asymmetric interlaced-pcr approach. atp-dependent helicase, cytochrome oxidase subunit i and ubiquitin-like activating e ... | 2015 | 25892913 |
| current technologies and related issues for mushroom transformation. | mushroom transformation requires a series of experimental steps, including generation of host strains with a desirable selective marker, design of vector dna, removal of host cell wall, introduction of foreign dna across the cell membrane, and integration into host genomic dna or maintenance of an autonomous vector dna inside the host cell. this review introduces limitations and obstacles related to transformation technologies along with possible solutions. current methods for cell wall removal ... | 2015 | 25892908 |
| overexpression of a gmcnx1 gene enhanced activity of nitrate reductase and aldehyde oxidase, and boosted mosaic virus resistance in soybean. | molybdenum cofactor (moco) is required for the activities of moco-dependant enzymes. cofactor for nitrate reductase and xanthine dehydrogenase (cnx1) is known to be involved in the biosynthesis of moco in plants. in this work, a soybean (glycine max l.) cnx1 gene (gmcnx1) was transferred into soybean using agrobacterium tumefaciens-mediated transformation method. twenty seven positive transgenic soybean plants were identified by coating leaves with phosphinothricin, bar protein quick dip stick a ... | 2015 | 25886067 |
| characterization of uronate dehydrogenases catalysing the initial step in an oxidative pathway. | uronate dehydrogenases catalyse the oxidation of uronic acids to aldaric acids, which represent 'top value-added chemicals' that have the potential to substitute petroleum-derived chemicals. the identification and annotation of three uronate dehydrogenases derived from fulvimarina pelagi htcc2506, streptomyces viridochromogenes dsm 40736 and oceanicola granulosus dsm 15982 via sequence analysis is described. characterization and comparison with two known uronate dehydrogenases in regard to subst ... | 2015 | 25884328 |
| [overexpression of laea enhances mevastatin production and reduces sporulation of penicillium citrinum]. | to study the regulation of laea overexpression on mevastatin production and sporulation in penicillium citrinum. | 2014 | 25876329 |
| unmasking host and microbial strategies in the agrobacterium-plant defense tango. | coevolutionary forces drive adaptation of both plant-associated microbes and their hosts. eloquently captured in the red queen hypothesis, the complexity of each plant-pathogen relationship reflects escalating adversarial strategies, but also external biotic and abiotic pressures on both partners. innate immune responses are triggered by highly conserved pathogen-associated molecular patterns, or pamps, that are harbingers of microbial presence. upon cell surface receptor-mediated recognition of ... | 2015 | 25873923 |
| spondylodiscitis due to rhizobium radiobacter. | 2015 | 25868625 | |
| [meristematic characteristics of tumors initiated by agrobacterium tumefaciens in pea plants]. | it is known that two key groups of plant hormones--auxins and cytokinins--play an important role in plant tumor development. the formation of agrobacterium-induced tumors results from the horizontal transfer of bacterial oncogenes involved in the biosynthesis of these hormones in the plant genome. the role of transcriptional factors in plant tumor development is poorly investigated. it can be assumed that tumor development associated with abnormal cell proliferation can be controlled by the same ... | 2015 | 25857193 |
| in vitro regeneration and agrobacterium tumefaciens-mediated genetic transformation in asakura-sanshoo (zanthoxylum piperitum (l.) dc. f. inerme makino) an important medicinal plant. | asakura-sanshoo (zanthoxylum piperitum [l.] dc. f. inerme makino) is an important medicinal plant in east asia. transgenic technique could be applied to improve plant traits and analyze gene function. however, there is no report on regeneration and genetic transformation in asakura-sanshoo. | 2017 | 25829778 |
| effect of leaf incubation temperature profiles on agrobacterium tumefaciens-mediated transient expression. | agrobacterium tumefaciens-mediated transient expression is known to be highly dependent on incubation temperature. compared with early studies that were conducted at constant temperature, we examined the effect of variable leaf incubation temperature on transient expression. as a model system, synthetic endoglucanase (e1) and endoxylanase (xyn10a) genes were transiently expressed in detached whole sunflower leaves via vacuum infiltration for biofuel applications. we found that the kinetics of tr ... | 2015 | 25829353 |
| comparison and evaluation of two diagnostic methods for detection of npt ii and gus genes in nicotiana tabacum. | to diminish the time required for some diagnostic assays including polymerase chain reaction (pcr), loop-mediated isothermal amplification (lamp) and also a visual detection protocol on the basis of npt ii and gus genes in transgenic tobacco plants were used. agrobacterium tumefaciens-mediated transformation of nicotiana tabacum leaf discs was performed with plant transformation vector of pbi 121. from kanamycin-resistant plants selected by their antibiotic resistance, four plants were selected ... | 2015 | 25820356 |
| transient plant transformation mediated by agrobacterium tumefaciens: principles, methods and applications. | agrobacterium tumefaciens is widely used as a versatile tool for development of stably transformed model plants and crops. however, the development of agrobacterium based transient plant transformation methods attracted substantial attention in recent years. transient transformation methods offer several applications advancing stable transformations such as rapid and scalable recombinant protein production and in planta functional genomics studies. herein, we highlight agrobacterium and plant ge ... | 2015 | 25819757 |
| a fungal transcription factor gene is expressed in plants from its own promoter and improves drought tolerance. | a fungal gene encoding a transcription factor is expressed from its own promoter in arabidopsis phloem and improves drought tolerance by reducing transpiration and increasing osmotic potential. horizontal gene transfer from unrelated organisms has occurred in the course of plant evolution, suggesting that some foreign genes may be useful to plants. the cthsr1 gene, previously isolated from the halophytic yeast candida tropicalis, encodes a heat-shock transcription factor-related protein. cthsr1, ... | 2015 | 25809153 |
| nicotine-degrading microorganisms and their potential applications. | nicotine-degrading microorganisms (ndms) are a special microbial group which can use nicotine as the sole carbon and nitrogen source for growth. since the 1950s, the bioconversion of nicotine by microbes has received increasing attention, and several ndms have been identified, such as arthrobacter nicotinovorans, microsporum gypseum, pellicularia filamentosa jts-208, and pseudomonas sp. 41. in recent years, increasing numbers of ndms have been isolated and identified from tobacco plantation soil ... | 2015 | 25805341 |
| expression of anti-tumor necrosis factor alpha (tnfα) single-chain variable fragment (scfv) in spirodela punctata plants transformed with agrobacterium tumefaciens. | therapeutic antibodies against tumor necrosis factor alpha (tnfα) have been considered effective for some of the autoimmune diseases such as rheumatoid arthritis, crohn's diseases, and so on. but associated limitations of the current therapeutics in terms of cost, availability, and immunogenicity have necessitated the need for alternative candidates. single-chain variable fragment (scfv) can negate the limitations tagged with the anti-tnfα therapeutics to a greater extent. in the present study, ... | 2016 | 25786575 |
| the class iii cyclobutane pyrimidine dimer photolyase structure reveals a new antenna chromophore binding site and alternative photoreduction pathways. | photolyases are proteins with an fad chromophore that repair uv-induced pyrimidine dimers on the dna in a light-dependent manner. the cyclobutane pyrimidine dimer class iii photolyases are structurally unknown but closely related to plant cryptochromes, which serve as blue-light photoreceptors. here we present the crystal structure of a class iii photolyase termed photolyase-related protein a (phra) of agrobacterium tumefaciens at 1.67-å resolution. phra contains 5,10-methenyltetrahydrofolate (m ... | 2015 | 25784552 |
| bacillus amyloliquefaciens strain 32a as a source of lipopeptides for biocontrol of agrobacterium tumefaciens strains. | a bacillus amyloliquefaciens strain, designated 32a, was used to identify new compounds active against agrobacterium tumefaciens and to evaluate their efficiency to control crown gall on carrot discs. | 2015 | 25764969 |
| rapid, high-throughput tracking of bacterial motility in 3d via phase-contrast holographic video microscopy. | tracking fast-swimming bacteria in three dimensions can be extremely challenging with current optical techniques and a microscopic approach that can rapidly acquire volumetric information is required. here, we introduce phase-contrast holographic video microscopy as a solution for the simultaneous tracking of multiple fast moving cells in three dimensions. this technique uses interference patterns formed between the scattered and the incident field to infer the three-dimensional (3d) position an ... | 2015 | 25762336 |
| quantitating plant microrna-mediated target repression using a dual-luciferase transient expression system. | microrna (mirna) mediated repression of target genes plays essential roles in a variety of functions in plants. an easy-to-use method that can effectively validate functional mirna-target interactions in plants thus is of particular interest. here, we describe an agrobacterium tumefaciens-mediated in vivo assay utilizing a dual-luciferase reporter system. with this method, the strength of mirna-mediated target repression can be quantified at both the mrna (via qrt-pcr) and protein (via dual-luci ... | 2015 | 25757778 |
| effects of tung oilseed fad2 and dgat2 genes on unsaturated fatty acid accumulation in rhodotorula glutinis and arabidopsis thaliana. | genetic engineering to produce valuable lipids containing unsaturated fatty acids (ufas) holds great promise for food and industrial applications. efforts to genetically modify plants to produce desirable ufas with single enzymes, however, have had modest success. the key enzymes fatty acid desaturase (fad) and diacylglycerol acyltransferase (dgat) are responsible for ufa biosynthesis (a push process) and assembling fatty acids into lipids (a pull process) in plants, respectively. to examine the ... | 2015 | 25754996 |
| molecular and phenotypic characterization of agrobacterium species from vineyards allows identification of typical agrobacterium vitis and atypical biovar 1 strains. | to molecularly and phenotypically characterize a selection of agrobacterium-like isolates from grapevine canes, crowns, soil and tumours in plants grown under cold conditions. | 2015 | 25752332 |
| characterization of the highly active fragment of glyceraldehyde-3-phosphate dehydrogenase gene promoter for recombinant protein expression in pleurotus ostreatus. | developing efficient native promoters is important for improving recombinant protein expression by fungal genetic engineering. the promoter region of glyceraldehyde-3-phosphate dehydrogenase gene in pleurotus ostreatus (pogpd) was isolated and optimized by upstream truncation. the activities of these promoters with different lengths were further confirmed by fluorescence, quantitative real-time pcr and western blot analysis. a truncated pogpd-p2 fragment (795 bp) drove enhanced green fluorescenc ... | 2015 | 25743073 |
| a functional genomics method for assaying gene function in phytopathogenic fungi through host-induced gene silencing mediated by agroinfiltration. | with the rapid growth of genomic information, there is an increasing demand for efficient analysis tools to study the function of predicted genes coded in genomes. agroinfiltration, the delivery of gene constructs into plant cells by agrobacterium tumefaciens infiltrated into leaves, is one such versatile, simple, and rapid technique that is increasingly used for transient gene expression assay in plants. in this chapter, we focus on the use of agroinfiltration as a functional genomics research ... | 2015 | 25740365 |
| a high-throughput rna interference (rnai)-based approach using hairy roots for the study of plant-rhizobia interactions. | legumes are major contributors to sustainable agriculture; their key feature is their ability to fix atmospheric nitrogen through symbiotic nitrogen fixation. legumes are often recalcitrant to regeneration and transformation by agrobacterium tumefaciens; however, a. rhizogenes-mediated root transformation and composite plant generation are rapid and convenient alternatives to study root biology, including root nodule symbiosis. rna interference (rnai), coupled with a. rhizogenes-mediated root tr ... | 2015 | 25740364 |
| transgene-induced gene silencing in plants. | rnai is the most important reverse genetics tool to trigger transgenic gene silencing, which is now applied widely to investigate gene function and also practically applied to enhance resistance to biotic and abiotic stress. recently, the most effective way to induce transgenic gene silencing is to introduce inverted repeat (ir) double-stranded rna (dsrna) or artificial microrna (amirna) instead of a transgenic sense or antisense strand of genes. the stable transgenic plants can be acquired thro ... | 2015 | 25740359 |
| against friend and foe: type 6 effectors in plant-associated bacteria. | bacterial secretion systems play critical roles in communication with neighboring bacteria and in the modulation of host immune responses via the secretion of small proteins called effectors. several secretion systems have been identified and these are denoted types i-vii. of these, the type vi secretion system (t6ss) and its effectors were only recently elucidated. most studies on the role and significance of the t6ss and its effectors have focused on human pathogens. in this review, type 6 eff ... | 2015 | 25732741 |
| plant cell death caused by fungal, bacterial, and viral elicitors: protective effect of mitochondria-targeted quinones. | chitosan (partially deacetylated chitin), a component of fungal cell walls, caused epidermal cell (ec) death in the leaves of pea (pisum sativum l.) and tobacco nicotiana tabacum or nicotiana benthamiana detected by destruction of cell nuclei. the mitochondria-targeted quinone skq1 prevented the destruction of ec nuclei induced by chitosan. chitosan increased and skq1 suppressed the activity of protein kinases in n. benthamiana and p. sativum and eliminated the effect of chitosan. chitosan induc ... | 2014 | 25716725 |
| novel pseudotaxis mechanisms improve migration of straight-swimming bacterial mutants through a porous environment. | bacterial locomotion driven by flagella is given directionality by the chemotaxis signal transduction network. in the classic plate assays of migration in porous motility agar, efficient motility is compromised in chemotaxis mutants of diverse bacteria. nonchemotactic mutants become trapped within the agar matrix. suppressor mutations that prevent this entanglement but do not restore chemotaxis, a phenomenon designated pseudotaxis, were first reported to arise for escherichia coli. in this study ... | 2015 | 25714707 |
| engineering isoprenoid biosynthesis in artemisia annua l. for the production of taxadiene: a key intermediate of taxol. | taxadiene is the first committed precursor to paclitaxel, marketed as taxol, arguably the most important anticancer agent against ovarian and breast cancer. in taxus, taxadiene is directly synthesized from geranylgeranyl diphosphate (ggpp) that is the common precursor for diterpenoids and is found in most plants and microbes. in this study, artemisia annua l., a chinese medicinal herb that grows fast and is rich in terpenoids, was used as a genetic engineering host to produce taxadiene. the txs ... | 2015 | 25705665 |
| rhizobium metallidurans sp. nov., a symbiotic heavy metal resistant bacterium isolated from the anthyllis vulneraria zn-hyperaccumulator. | a gram-stain-negative, aerobic, rod-shaped, non-spore-forming bacterium (chimec512(t)) was isolated from 56 host seedlings of the hyperaccumulating anthyllis vulneraria legume, which was on an old zinc mining site at les avinières, saint-laurent-le-minier, gard, south of france. on the basis of 16s rrna gene sequence similarities, strain chimec512(t) was shown to belong to the genus rhizobium and to be most closely related to rhizobium endophyticum ccge 2052(t) (98.4%), rhizobium tibeticum ccbau ... | 2015 | 25701848 |
| a novel sterol regulatory element-binding protein gene (srea) identified in penicillium digitatum is required for prochloraz resistance, full virulence and erg11 (cyp51) regulation. | penicillium digitatum is the most destructive postharvest pathogen of citrus fruits, causing fruit decay and economic loss. additionally, control of the disease is further complicated by the emergence of drug-resistant strains due to the extensive use of triazole antifungal drugs. in this work, an orthologus gene encoding a putative sterol regulatory element-binding protein (srebp) was identified in the genome of p. digitatum and named srea. the putative srea protein contains a conserved domain ... | 2015 | 25699519 |
| optimization of chlorella vulgaris and bioflocculant-producing bacteria co-culture: enhancing microalgae harvesting and lipid content. | microalgae are a sustainable bioresource, and the biofuel they produce is widely considered to be an alternative to limited natural fuel resources. however, microalgae harvesting is a bottleneck in the development of technology. axenic chlorella vulgaris microalgae exhibit poor harvesting, as expressed by a flocculation efficiency of 0·2%. this work optimized the co-culture conditions of c. vulgaris and bioflocculant-producing bacteria in synthetic wastewater using response surface methodology ( ... | 2015 | 25693426 |
| exploring the enantioselective mechanism of halohydrin dehalogenase from agrobacterium radiobacter ad1 by iterative saturation mutagenesis. | halohydrin dehalogenase from agrobacterium radiobacter ad1 (hhec) shows great potential in producing valuable chiral epoxides and β-substituted alcohols. the wild-type (wt) enzyme displays a high r-enantiopreference toward most aromatic substrates, whereas no s-selective hhec has been reported to date. to obtain more enantioselective enzymes, seven noncatalytic active-site residues were subjected to iterative saturation mutagenesis (ism). after two rounds of screening aspects of both activity an ... | 2015 | 25681194 |
| aboveground insect infestation attenuates belowground agrobacterium-mediated genetic transformation. | agrobacterium tumefaciens causes crown gall disease. although agrobacterium can be popularly used for genetic engineering, the influence of aboveground insect infestation on agrobacterium induced gall formation has not been investigated. nicotiana benthamiana leaves were exposed to a sucking insect (whitefly) infestation and benzothiadiazole (bth) for 7 d, and these exposed plants were inoculated with a tumorigenic agrobacterium strain. we evaluated, both in planta and in vitro, how whitefly inf ... | 2015 | 25676198 |
| rhizobium pusense is the main human pathogen in the genus agrobacterium/rhizobium. | rhizobium pusense was recently described after isolation from the rhizosphere of chickpea. multilocus sequence-based analysis of clinical isolates identified as agrobacterium (rhizobium) radiobacter demonstrated that r. pusense is the main human pathogen within agrobacterium (rhizobium) spp. clinical microbiology of agrobacterium (rhizobium) should be considered in the light of recent taxonomic changes. | 2015 | 25669878 |
| a single point mutation enhances hydroxynitrile synthesis by halohydrin dehalogenase. | the cyanide-mediated ring opening of epoxides catalyzed by halohydrin dehalogenases yields β-hydroxynitriles that are of high interest for synthetic chemistry. the best studied halohydrin dehalogenase to date is the enzyme from agrobacterium radiobacter, but this enzyme (hhec) exhibits only low cyanolysis activities. sequence comparison between a pair of related halohydrin dehalogenases from corynebacterium and mycobacterium suggested that substitution of a threonine that interacts with the acti ... | 2015 | 25659632 |
| a simple and efficient genetic transformation method of ganoderma weberianum. | in this study, the agrobacterium tumefaciens-mediated transformation method for ganderma weberianum has been established. driven by the cauliflower mosaic virus (camv) 35s promoter, the hygromycin phosphotransferase (hpt), β-glucuronidase (uida), and enhanced green fluorescent protein (egfp) genes have been efficiently expressed in transgenic mycelia and spores. the transformation system was composed of the growing mycelia, a. tumefaciens strain gv3101, and the expression vector pbi-h1, harborin ... | 2015 | 25655754 |
| comparative whole-genome analyses of selection marker-free rice-based cholera toxin b-subunit vaccine lines and wild-type lines. | we have developed a rice-based oral cholera vaccine named mucorice-ctb (cholera toxin b-subunit) by using an agrobacterium tumefaciens-mediated co-transformation system. to assess the genome-wide effects of this system on the rice genome, we compared the genomes of three selection marker-free mucorice-ctb lines with those of two wild-type rice lines (oryza sativa l. cv. nipponbare). mutation profiles of the transgenic and wild-type genomes were examined by next-generation sequencing (ngs). | 2015 | 25653106 |
| identification of pathogenicity-related genes in the rice pathogen ustilaginoidea virens through random insertional mutagenesis. | agrobacterium tumefaciens-mediated transformation (atmt) is becoming a popular effective system as an insertional mutagenesis tool in filamentous fungi. to gain more insight into the cellular and molecular mechanisms in the pathogenesis of ustilaginoidea virens, the causal agent of rice false smut disease, a t-dna insertion mutant library of u. virens was established using atmt. we optimized a range of conditions to improve the transformation efficiency. transformants were most effectively obtai ... | 2015 | 25636735 |