Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| evaluation of the neutralising capacity of bactec medium for some antibiotics. | bactec medium 9240 (becton dickinson, md, usa) is a blood culture medium used routinely at sultan qaboos university hospital (squh). the medium is said to contain substances that can neutralise antibiotics and destroy leucocytes in blood samples. in this study, the ability of the medium to neutralise the effect of some antibiotics and to destroy leucocytes is investigated. vancomycin, amoxicillin, chloramphenicol, penicillin, gentamicin, fungizone and amikacin at various concentrations were adde ... | 2004 | 28696902 |
| optimised chronic infection models demonstrate that siderophore 'cheating' in pseudomonas aeruginosa is context specific. | the potential for siderophore mutants of pseudomonas aeruginosa to attenuate virulence during infection, and the possibility of exploiting this for clinical ends, have attracted much discussion. this has largely been based on the results of in vitro experiments conducted in iron-limited growth medium, in which siderophore mutants act as social 'cheats:' increasing in frequency at the expense of the wild type to result in low-productivity, low-virulence populations dominated by mutants. we show t ... | 2017 | 28696423 |
| pseudomonas aeruginosa clinical isolates in nepal coproducing metallo-β-lactamases and 16s rrna methyltransferases. | a total of 11 multidrug-resistant pseudomonas aeruginosa clinical isolates were obtained in nepal. four of these isolates harbored genes encoding one or more carbapenemases (dim-1, ndm-1, and/or vim-2), and five harbored genes encoding a 16s rrna methyltransferase (rmtb4 or rmtf2). a novel rmtf variant, rmtf2, had a substitution (k65e) compared with the same gene in rmtf. to our knowledge, this is the first report describing carbapenemase- and 16s rrna methyltransferase-coproducing p. aeruginosa ... | 2017 | 28696242 |
| community composition determines activity of antibiotics against multispecies biofilms. | in young cystic fibrosis (cf) patients, staphylococcus aureus is typically the most prevalent organism, while in adults, pseudomonas aeruginosa is the major pathogen. more recently, it was observed that also streptococcus anginosus plays an important role in exacerbations of respiratory symptoms. these species are often coisolated from cf lungs, yet little is known about whether antibiotic killing of one species is influenced by the presence of others. in the present study, we compared the activ ... | 2017 | 28696232 |
| crystal structure of the n-terminal domain of vqsr from pseudomonas aeruginosa at 2.1 å resolution. | vqsr is a quorum-sensing (qs) transcriptional regulator which controls qs systems (las, rhl and pqs) by directly downregulating the expression of qscr in pseudomonas aeruginosa. as a member of the luxr family of proteins, vqsr shares the common motif of a helix-turn-helix (hth)-type dna-binding domain at the c-terminus, while the function of its n-terminal domain remains obscure. here, the crystal structure of the n-terminal domain of vqsr (vqsr-n; residues 1-193) was determined at a resolution ... | 2017 | 28695853 |
| investigating the interaction of octapeptin a3 with model bacterial membranes. | octapeptins are cyclic lipopeptides with a broader spectrum of activity against fungi and polymyxin-resistant gram-negative and gram-positive bacteria. in the present study, we investigated the interaction of octapeptin a3 with asymmetric outer membrane models of gram-negative pathogen pseudomonas aeruginosa using neutron reflectometry, together with fluorimetric and calorimetry methods. for the first time, our neutron reflectometry results reveal that the interaction of octapeptin a3 with the g ... | 2017 | 28695731 |
| simultaneous valorization and biocatalytic upgrading of heavy vacuum gas oil by the biosurfactant-producing pseudomonas aeruginosa ak6u. | heavy vacuum gas oil (hvgo) is a complex and viscous hydrocarbon stream that is produced as the bottom side product from the vacuum distillation units in petroleum refineries. hvgo is conventionally treated with thermochemical process, which is costly and environmentally polluting. here, we investigate two petroleum biotechnology applications, namely valorization and bioupgrading, as green approaches for valorization and upgrading of hvgo. the pseudomonas aeruginosa ak6u strain grew on 20% v/v o ... | 2017 | 28695623 |
| risk factors for acquisition of fluoroquinolone or aminoglycoside resistance in addition to carbapenem resistance in pseudomonas aeruginosa. | carbapenems, fluoroquinolones (fqs), and aminoglycosides (ags) are key drugs for treating pseudomonas aeruginosa infections, and accumulation of drug resistances make antibiotic therapy difficult. | 2017 | 28694882 |
| chemical composition and cytotoxic and antibacterial activities of the essential oil of aloysia citriodora palau grown in morocco. | the aim of this work is to investigate the in vitro cytotoxic and antibacterial effects of the essential oils of aloysia citriodora palau, harvested in different regions of morocco. the chemical profile was established using gas chromatography-mass spectrometry analysis. the cytotoxic activity against p815, mcf7, and vero cell lines as well as the normal human peripheral blood mononuclear cells (pbmcs) was evaluated using the mtt assay. standard, atcc, strains of bacteria (escherichia coli, stap ... | 2017 | 28694822 |
| antivirulent properties of underexplored cinnamomum tamala essential oil and its synergistic effects with dnase against pseudomonas aeruginosa biofilms - an in vitro study. | pseudomonas aeruginosa is a nosocomial pathogen colonizing patients with chronic infectious diseases and has gained resistance to all the known broad spectrum antibiotics available today. the present study showcases the antibiofilm potential of an essential oil (eo) from an underexplored cinnamomum species namely, c. tamala, against p. aeruginosa biofilms. furthermore, the synergistic effects of the eo along with a commercially available dnase (dnasei) and a dnase (mbd) isolated from a marine ba ... | 2017 | 28694794 |
| role of biofilm in cerebrospinal fluid shunt infections: a study at tertiary neurocare center from south india. | biofilms are the source of persistent infections of many pathogenic microbes. they are responsible for nosocomial infection and also associated with many surgical conditions including indwelling medical devices such as ventriculoperitoneal shunt. a significant problem encountered in shunt procedures is obstruction followed by infection, with infection rate ranging from 2% to 27%, often with poor outcome. | 2017 | 28694609 |
| impact of broad-spectrum antimicrobial treatment on the ecology of intestinal flora. | suppression of intestinal flora by broad-spectrum antimicrobial agents facilitated risk of colonization or infection with resistant pathogen. we aimed to investigate the changes in bowel carriage of target resistant microorganisms (tro) among patients treated with three different classes of pseudomonas-sparing broad-spectrum antimicrobial agents (ertapenem, moxifloxacin and flomoxef) with anaerobic coverage. risk factors for developing colonization of tro were also analyzed. | 2017 | 28693929 |
| comparison of in-house and commercial real time-pcr based carbapenemase gene detection methods in enterobacteriaceae and non-fermenting gram-negative bacterial isolates. | carbapenemase-producing gram-negative bacteria are increasing globally and have been associated with outbreaks in hospital settings. thus, the accurate detection of these bacteria in infections is mandatory for administering the adequate therapy and infection control measures. this study aimed to establish and evaluate a multiplex real-time pcr assay for the simultaneous detection of carbapenemase gene variants in gram-negative rods and to compare the performance with a commercial rt-pcr assay ( ... | 2017 | 28693493 |
| n-(3-oxododecanoyl)-l-homoserine lactone interactions in the breast tumor microenvironment: implications for breast cancer viability and proliferation in vitro. | it is well documented that the tumor microenvironment profoundly impacts the etiology and progression of breast cancer, yet the contribution of the resident microbiome within breast tissue remains poorly understood. tumor microenvironmental conditions, such as hypoxia and dense tumor stroma, predispose progressive phenotypes and therapy resistance, however the role of bacteria in this interplay remains uncharacterized. we hypothesized that the effect of individual bacterial secreted molecules on ... | 2017 | 28692660 |
| diversity, prevalence, and longitudinal occurrence of type ii toxin-antitoxin systems of pseudomonas aeruginosa infecting cystic fibrosis lungs. | type ii toxin-antitoxin (ta) systems are most commonly composed of two genes encoding a stable toxin, which harms the cell, and an unstable antitoxin that can inactivate it. ta systems were initially characterized as selfish elements, but have recently gained attention for regulating general stress responses responsible for pathogen virulence, formation of drug-tolerant persister cells and biofilms-all implicated in causing recalcitrant chronic infections. we use a bioinformatics approach to exp ... | 2017 | 28690609 |
| bronchoalveolar lavage microvesicles protect burn-injured mice from pulmonary infection. | pseudomonas aeruginosa is a major cause of morbidity and mortality among burn patients, despite antibiotic therapy. there is a need to identify innate immune defenses that prevent p aeruginosa infection in injured adults in an effort to find therapeutic alternatives to antibiotics. here, we tested our hypothesis that microvesicles (mvs) in bronchoalveolar (bal) fluid have a role in the immunity of the lung in response to pathogens. | 2017 | 28690205 |
| investigating the variation in the incidence of new pseudomonas aeruginosa infection between paediatric cystic fibrosis centres. | 2017 | 28690130 | |
| arginase purified from endophytic pseudomonas aeruginosa ih2: induce apoptosis through both cell cycle arrest and mmp loss in human leukemic hl-60 cells. | arginase is a therapeutic enzyme for arginine-auxotrophic cancers but their low anticancer activity, less proteolytic tolerance and shorter serum half-life are the major shortcomings. in this study, arginase from pseudomonas aeruginosa ih2 was purified to homogeneity and estimated as 75 kda on native-page and 37 kda on sds-page. arginase showed optimum activity at ph 8 and temperature 35 °c. mn(2+) and mg(2+) ions enhanced arginase activity while, li(+), cu(2+), and al(3+) ions reduced arginase ... | 2017 | 28690100 |
| bioactivity of ceftazidime and fluconazole included in polymethyl methacrylate bone cement for use in arthroplasty. | the microorganisms that most frequently cause prosthetic joint infection are methicillin-resistant staphylococcus aureus and gram-negative aerobic bacillus. studies have documented the efficacy of mixing antibiotics with polymethyl methacrylate, but that of antifungal drugs has not received much attention. the objective of this in vitro study was to characterize the elution profile and bioactivity of ceftazidime and fluconazole when incorporated into bone cement in proportions intended for proph ... | 2017 | 28690040 |
| in vitro activity of ceftolozane/tazobactam in combination with other classes of antibacterial agents. | combination antibiotic therapy has been used successfully to treat some patients with bacterial infections. however, although certain combinations may result in beneficial synergistic activity, others may produce antagonistic effects resulting in poor treatment outcomes. ceftolozane/tazobactam is an antibacterial agent with potent activity against pseudomonas aeruginosa and many other gram-negative pathogens, including extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae. this study ... | 2017 | 28689923 |
| investigation and analysis of the characteristics and drug sensitivity of bacteria in skin ulcer infections. | skin ulcer is a common type of disease affecting patients' health and quality of life, and bacterial infection increases the difficulty of its management. | 2017 | 28689800 |
| selective biosensing of staphylococcus aureus using chitosan quantum dots. | selective biosensing of staphylococcus aureus (s. aureus) using chitosan modified quantum dots (cts@cds qds) in the presence of hydrogen peroxide is reported. the method is based on the intrinsic positive catalase activity of s. aureus. cts@cds quantum dots provide high dispersion in aqueous media with high fluorescence emission. staphylococcus aureus causes a selective quenching of the fluorescence emission of cts@cds qds in the presence of h2o2 compared to other pathogens such as escherichia c ... | 2017 | 28689078 |
| bactericidal activity of self-assembled palmitic and stearic fatty acid crystals on highly ordered pyrolytic graphite. | the wings of insects such as cicadas and dragonflies have been found to possess nanostructure arrays that are assembled from fatty acids. these arrays can physically interact with the bacterial cell membranes, leading to the death of the cell. such mechanobactericidal surfaces are of significant interest, as they can kill bacteria without the need for antibacterial chemicals. here, we report on the bactericidal effect of two of the main lipid components of the insect wing epicuticle, palmitic (c ... | 2017 | 28688988 |
| effect of a pseudomonas fluorescens tailocin against phytopathogenic xanthomonas observed by atomic force microscopy. | phage tail-like bacteriocins, called tailocins, represent a class of protein complexes produced by a multitude of bacteria. pseudomonas fluorescens sf4c, a strain isolated from wheat rhizosphere, produces a bacteriocin similar to phage tail-like pyocins of pseudomonas aeruginosa. this tailocin has antimicrobial activity against several phytopathogenic strains of the genus xanthomonas and pseudomonas. in this work, the effect of the sf4c tailocin on the phytopathogenic strain x. axonopodis pv ves ... | 2017 | 28687515 |
| in vitro and in vivo antimicrobial activity of a synthetic peptide derived from the c-terminal region of human chemokine ccl13 against pseudomonas aeruginosa. | chemokines are important mediators of immunological responses during inflammation and under steady-state conditions. in addition to regulating cell migration, some chemotactic cytokines have direct effects on bacteria. here, we characterized the antibacterial ability of the synthetic oligopeptide ccl1357-75, which corresponds to the carboxyl-terminal region of the human chemokine ccl13. in vitro measurements indicated that ccl1357-75 disrupts the cell membrane of pseudomonas aeruginosa through a ... | 2017 | 28687455 |
| the pseudomonas aeruginosa crcz rna interferes with hfq-mediated riboregulation. | the rna chaperone hfq regulates virulence and metabolism in the opportunistic pathogen pseudomonas aeruginosa. during carbon catabolite repression (ccr) hfq together with the catabolite repression control protein crc can act as a translational repressor of catabolic genes. upon relief of ccr, the level of the hfq-titrating rna crcz is increasing, which in turn abrogates hfq-mediated translational repression. as the interdependence of hfq-mediated and rna based control mechanisms is poorly unders ... | 2017 | 28686727 |
| exploration of phytoconstituents from mussaenda roxburghii and studies of their antibiofilm effect. | in the context of ethno botanical importance with no phytochemical investigations, mussaenda roxburghii have been investigated to explore it's phytoconstituents and studies of their antibiofilm activity. four compounds have been isolated from the aerial parts of this plant and were characterized as 2α, 3β, 19α, 23-tetrahydroxyurs-12-en-28-oic acid (1), β-sitosterol glucoside (4), lupeol palmitate (5) and myoinositol (6). all these compounds were tested for antibacterial and antibiofilm activity ... | 2017 | 28686323 |
| reorganization of gene network for degradation of polycyclic aromatic hydrocarbons (pahs) in pseudomonas aeruginosa pao1 under several conditions. | although polycyclic aromatic hydrocarbons (pahs) are harmful to human health, their elimination from the environment is not easy. biodegradation of pahs is promising since many bacteria have the ability to use hydrocarbons as their sole carbon and energy sources for growth. of various microorganisms that can degrade pahs, pseudomonas aeruginosa is particularly important, not only because it causes a series of diseases including infection in cystic fibrosis patients, but also because it is a mode ... | 2017 | 28685384 |
| mechanistic action of weak acid drugs on biofilms. | selective permeability of a biofilm matrix to some drugs has resulted in the development of drug tolerant bacteria. here we studied the efficacy of a weak organic acid drug, n-acetyl-l-cysteine (nac), on the eradication of biofilms formed by the mucoid strain of pseudomonas aeruginosa and investigated the commonality of this drug with that of acetic acid. we showed that nac and acetic acid at ph < pka can penetrate the matrix and eventually kill 100% of the bacteria embedded in the biofilm. once ... | 2017 | 28684849 |
| modifications of pseudomonas aeruginosa cell envelope in the cystic fibrosis airway alters interactions with immune cells. | pseudomonas aeruginosa is a ubiquitous environmental organism and an opportunistic pathogen that causes chronic lung infections in the airways of cystic fibrosis (cf) patients as well as other immune-compromised individuals. during infection, p. aeruginosa enters the terminal bronchioles and alveoli and comes into contact with alveolar lining fluid (alf), which contains homeostatic and antimicrobial hydrolytic activities, termed hydrolases. these hydrolases comprise an array of lipases, glycosid ... | 2017 | 28684799 |
| draft genome sequence of an imp-7-producing pseudomonas aeruginosa bloodstream infection isolate from australia. | imp-7 is one of the two imp-type carbapenemases that in pseudomonas aeruginosa are not limited to a geographic area, but it has not been previously reported in the australian setting. we report here the draft genome sequence of an australian p. aeruginosa bloodstream infection isolate that contains imp-7. | 2017 | 28684579 |
| macrolides protect against pseudomonas aeruginosa infection via inhibition of inflammasomes. | background macrolides antibiotics have been used effectively in many chronic diseases, especially with pseudomonas aeruginosa (p. aeruginosa) infection. the mechanisms underlying the therapeutic effects of macrolides in these diseases remain poorly understood. methods we established a mouse model of chronic lung infection using p. aeruginosa agar-beads, with azithromycin treatment or placebo. lung injury, bacterial clearance and inflammasome-related proteins were measured. in vitro, the inflamma ... | 2017 | 28684545 |
| bacterial agents and antibiotic resistance profiles of infections from different sites that occurred among patients at debre markos referral hospital, ethiopia: a cross-sectional study. | in developing countries like ethiopia, infections with antibiotic resistant bacteria become a real threat. hence, monitoring of local level antimicrobial resistance profile is indispensable to contain the spread of drug resistant bacteria and intervene poor awareness on antimicrobial resistance. therefore, this study aimed at determining bacterial and antibiotic resistance profiles of infections from different sites that occurred among patients. | 2017 | 28683780 |
| thymus vulgaris l. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity. | this study evaluated the biological effects of the t. vulgaris l. extract., such as antimicrobial activity on planktonic cultures and mono- and polymicrobial biofilms, cytotoxicity, anti-inflammatory activity and genotoxicity. | 2017 | 28683409 |
| fine structure analysis of lipopolysaccharides in bacteriophage-resistant pseudomonas aeruginosa pao1 mutants. | pseudomonas aeruginosa lipopolysaccharides (lps) serve as primary receptors for many bacteriophages and, consequently, their biosynthesis is frequently affected in phage-resistant mutants. we previously isolated phage-resistant pao1 mutants using three different phages, and showed that they were affected in the synthesis of lps. here we have investigated in detail the effect of mutations in seven genes involved in different steps of the production of core and oligosaccharide chains. the band pro ... | 2017 | 28682742 |
| selective and high dynamic range assay format for multiplex detection of pathogenic pseudomonas aeruginosa, salmonella typhimurium, and legionella pneumophila rnas using surface plasmon resonance imaging. | due to its well-characterized and highly conserved structure, as well as its relative abundance in metabolically active cells, bacterial 16s rrna sequence plays an important role in microbial identification. in this work, a biosensing strategy has been developed for simultaneous detection of 16s rrna analytes of three pathogenic bacterial strains: legionella pneumophila, pseudomonas aeruginosa, and salmonella typhimurium. surface plasmon resonance imaging (spri) was used as a detection technique ... | 2017 | 28682593 |
| effectiveness of topical application of ostrich oil on the healing of staphylococcus aureus- and pseudomonas aeruginosa-infected wounds. | management of infected wounds is one of the major challenges that surgeons and nurses face. several antimicrobial agents have been used, but the toxicity, drug resistance, and their effect on the healing process remain a matter of concern. the present study was designed to analyze the accelerative impact of topical application of ostrich oil on infected wounds in a mouse model. | 2017 | 28682114 |
| the respiratory threat posed by multidrug resistant gram-negative bacteria. | respiratory infections are a major cause of global mortality and morbidity. in recent years, an increased incidence of multidrug-resistant (mdr) gram-negative bacteria (gnb) has been described. microorganisms such as pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae or acinetobacter baumannii have been identified as causative pathogens of different respiratory tract infections. several studies have detected mdr-gnb in patients with community-acquired and nosocomial pneumonia. furth ... | 2017 | 28681941 |
| emergence of rmtc and rmtf 16s rrna methyltransferase in clinical isolates of pseudomonas aeruginosa. | occurrence of aminoglycoside (ag) resistance in clinical isolates of pseudomonas aeruginosa is investigated in this study. antimicrobial susceptibility test and minimum inhibitory concentration (mic) for amikacin and gentamicin were performed followed by polymerase chain reaction amplifications of ag modifying enzyme genes (aac(6´)-i, aac(6´)-ii, aac(3)-ii/vi, ant(2´´)-i, aph(3´)-vi) and 16s methylases (rmta-d, rmtf and arma). mic50and mic90were 64, 128 and > 256, >256 for amikacin and gentamici ... | 2017 | 28681822 |
| assessment of surgical risk factors in the development of ventilator-associated pneumonia in neurosurgical intensive care unit patients: alarming observations. | ventilator-associated pneumonia (vap) is the most frequent nosocomial infection in patients receiving mechanical ventilation (mv) and contributes to a longer intensive care unit (icu) stay, duration of mv, and a high morbidity and mortality. | 2017 | 28681751 |
| top-down lesa mass spectrometry protein analysis of gram-positive and gram-negative bacteria. | we have previously shown that liquid extraction surface analysis (lesa) mass spectrometry (ms) is a technique suitable for the top-down analysis of proteins directly from intact colonies of the gram-negative bacterium escherichia coli k-12. here we extend the application of lesa ms to gram-negative pseudomonas aeruginosa ps1054 and gram-positive staphylococcus aureus mssa476, as well as two strains of e. coli (k-12 and bl21 mcherry) and an unknown species of staphylococcus. moreover, we demonstr ... | 2017 | 28681361 |
| association of silver nanoparticles and curcumin solid dispersion: antimicrobial and antioxidant properties. | the last century, more precisely after 1945, was marked by major advances in the treatment of infectious diseases which promoted a decrease in mortality and morbidity. despite these advances, currently the development of antimicrobial resistance has been growing drastically and therefore there is a pressing need to search for new compounds. silver nanoparticles (agnps) have been demonstrating good antimicrobial activity against different bacteria, viruses, and fungi. curcumin (cur) extracted fro ... | 2017 | 28681332 |
| nbiochip, a lab-on-a-chip platform of mono- and polymicrobial biofilms for high-throughput downstream applications. | current in vitro techniques for the culture of microorganisms, and particularly of delicate microbial biofilms, are still mostly limited to low-density plates and manual labor and are not amenable to automation and true high-throughput (ht) applications. we have developed a novel fully automated platform for the formation of mono- and polymicrobial biofilms of staphylococcus aureus, pseudomonas aeruginosa, and candida albicans at the nanoscale level. the nbiochip is robotically printed, robustly ... | 2017 | 28680970 |
| biofilm-induced type 2 innate immunity in a cystic fibrosis model of pseudomonas aeruginosa. | biofilm-producing strains of pseudomonas aeruginosa are a major cause of morbidity and mortality in cystic fibrosis (cf) patients. in these patients, increased levels of il-17 as well as of il-5 and il-13 along with arginase (arg)-positive macrophages have been observed in bronchoalveolar lavage fluid. while il-17 is a strong proinflammatory cytokine associated with host defense against bacterial and fungal infections and is also elevated in several autoimmune diseases, il-5/il-13 and arg1-posit ... | 2017 | 28680858 |
| antimicrobial ceragenins inhibit biofilms and affect mammalian cell viability and migration in vitro. | the healing of burn wounds is often hampered by bacterial infection and the formation of biofilms. antimicrobial peptides (amps) are effective in promoting wound healing, but are susceptible to degradation. we have tested the ability of ceragenins (csas), mimics of antimicrobial peptides, to mitigate preformed biofilms and stimulate wound healing in vitro. potent csas (mics < 10 μg·ml(-1)) were tested against biofilms formed from a mixture of pseudomonas aeruginosa and staphylococcus aureus grow ... | 2017 | 28680809 |
| proteomic profile of cystic fibrosis sputum cells in adults chronically infected with pseudomonas aeruginosa. | lung disease is the main cause of morbidity and mortality in cystic fibrosis (cf), and involves chronic infection and perturbed immune responses. tissue damage is mediated mostly by extracellular proteases, but other cellular proteins may also contribute to damage through their effect on cell activities and/or release into sputum fluid by means of active secretion or cell death.we employed mudpit (multidimensional protein identification technology) to identify sputum cellular proteins with consi ... | 2017 | 28679606 |
| characteristics of a pseudomonas aeruginosa induced porcine sepsis model for multi-organ metabolic flux measurements. | survival of sepsis is related to loss of muscle mass. therefore, it is imperative to further define and understand the basic alterations in nutrient metabolism in order to improve targeted sepsis nutritional therapies. we developed and evaluated a controlled hyperdynamic severe sepsis pig model that can be used for in vivo multi-organ metabolic studies in a conscious state. in this catheterized pig model, bacteremia was induced intravenously with 10(9) cfu/h pseudomonas aeruginosa (pa) in 13 pig ... | 2017 | 28679339 |
| pseudomonas aeruginosa cells attached to a surface display a typical proteome early as 20 minutes of incubation. | biofilms are present in all environments and often result in negative effects due to properties of the biofilm lifestyle and especially antibiotics resistance. biofilms are associated with chronic infections. controlling bacterial attachment, the first step of biofilm formation, is crucial for fighting against biofilm and subsequently preventing the persistence of infection. thus deciphering the underlying molecular mechanisms involved in attachment could allow discovering molecular targets from ... | 2017 | 28678862 |
| super-cationic carbon quantum dots synthesized from spermidine as an eye drop formulation for topical treatment of bacterial keratitis. | we have developed a one-step method to synthesize carbon quantum dots (cqdpas) from biogenic polyamines (pas) as an antibacterial agent for topical treatment of bacterial keratitis (bk). cqds synthesized by direct pyrolysis of spermidine (spd) powder through a simple dry heating treatment exhibit a solubility and yield much higher than those from putrescine and spermine. we demonstrate that cqds obtained from spds (cqdspds) possess effective antibacterial activities against non-multidrug-resista ... | 2017 | 28677399 |
| expression, purification, and characterization of a membrane-bound d-amino acid dehydrogenase from proteus mirabilis jn458. | to characterize a novel membrane-bound d -amino acid dehydrogenase from proteus mirabilis jn458 (pmdad). | 2017 | 28676939 |
| interplay between 4-hydroxy-3-methyl-2-alkylquinoline and n-acyl-homoserine lactone signaling in a burkholderia cepacia complex clinical strain. | species from the burkholderia cepacia complex (bcc) share a canonical luxi/luxr quorum sensing (qs) regulation system named cepi/cepr, which mainly relies on the acyl-homoserine lactone (ahl), octanoyl-homoserine lactone (c8-hsl) as signaling molecule. burkholderia ambifaria is one of the least virulent bcc species, more often isolated from rhizospheres where it exerts a plant growth-promoting activity. however, clinical strains of b. ambifaria display distinct features, such as phase variation ... | 2017 | 28676791 |
| structural insights into the mechanism of the membrane integral n-acyltransferase step in bacterial lipoprotein synthesis. | lipoproteins serve essential roles in the bacterial cell envelope. the posttranslational modification pathway leading to lipoprotein synthesis involves three enzymes. all are potential targets for the development of new antibiotics. here we report the crystal structure of the last enzyme in the pathway, apolipoprotein n-acyltransferase, lnt, responsible for adding a third acyl chain to the lipoprotein's invariant diacylated n-terminal cysteine. structures of lnt from pseudomonas aeruginosa and e ... | 2017 | 28675161 |
| titanium surfaces immobilized with the major antimicrobial fragment fk-16 of human cathelicidin ll-37 are potent against multiple antibiotic-resistant bacteria. | infections on implanted medical devices are a challenging problem, especially when bacteria form difficult-to-treat biofilms. antimicrobial peptides are considered to be a solution due to their potency against antibiotic-resistant superbugs. previously, the authors' laboratory demonstrated the prevention of staphylococcal biofilm formation in an animal catheter model by injecting merecidin (formerly known as 17biphe2), a peptide engineered based on the only human cathelicidin. this study documen ... | 2017 | 28675109 |
| a rapid and simple method for identifying bacterial polar lipid components in wet biomass. | there are marked differences between wet and freeze-dried cells with regard to the identification of polar lipid components. the determination of the polar lipid composition of freeze-dried cells is well established. however, several approaches to identifying polar lipid components in wet cells have met with limited success owing to the presence of non-polar compounds in the extracts, resulting in a lipid composition with a narrow scope. in this study, we surveyed the lipid profiles of the wet b ... | 2017 | 28674971 |
| d-bmap18 antimicrobial peptide is active in vitro, resists to pulmonary proteases but loses its activity in a murine model of pseudomonas aeruginosa lung infection. | the spread of antibiotic resistant-pathogens is driving the search for new antimicrobial compounds. pulmonary infections experienced by cystic fibrosis (cf) patients are a dramatic example of this health-care emergency. antimicrobial peptides could answer the need for new antibiotics but translating them from basic research to the clinic is a challenge. we have previously evaluated the potential of the small membranolytic peptide bmap-18 to treat cf-related infections, discovering that while thi ... | 2017 | 28674688 |
| in vivo emergence of resistance to novel cephalosporin-β-lactamase inhibitor combinations through the duplication of amino acid d149 from oxa-2 β-lactamase (oxa-539) in sequence type 235 pseudomonas aeruginosa. | resistance development to novel cephalosporin-β-lactamase inhibitor combinations during ceftazidime treatment of a surgical infection by pseudomonas aeruginosa was investigated. both initial (97c2) and final (98g1) isolates belonged to the high-risk clone sequence type (st) 235 and were resistant to carbapenems (oprd), fluoroquinolones (gyra-t83i, parc-s87l), and aminoglycosides (aaca7/aaca8/aada6). 98g1 also showed resistance to ceftazidime, ceftazidime-avibactam, and ceftolozane-tazobactam. se ... | 2017 | 28674059 |
| rsma and amrz orchestrate the assembly of all three type vi secretion systems in pseudomonas aeruginosa. | the type vi secretion system (t6ss) is a weapon of bacterial warfare and host cell subversion. the gram-negative pathogen pseudomonas aeruginosa has three t6sss involved in colonization, competition, and full virulence. h1-t6ss is a molecular gun firing seven toxins, tse1-tse7, challenging survival of other bacteria and helping p. aeruginosa to prevail in specific niches. the h1-t6ss characterization was facilitated through studying a p. aeruginosa strain lacking the rets sensor, which has a ful ... | 2017 | 28673999 |
| correction for rodesney et al., mechanosensing of shear by pseudomonas aeruginosa leads to increased levels of the cyclic-di-gmp signal initiating biofilm development. | 2017 | 28673990 | |
| characterization of first hemin-requiring pseudomonas aeruginosa small-colony variants from the blood of an octogenarian male-patient with double pneumonitis. | a hemin-requiring pseudomonas aeruginosa small-colony variant (scv) was isolated from the blood of an octogenarian male-patient with double pneumonitis. the isolate was capable of growing on both sheep blood and chocolate agars but not on macconkey agars without blood ingredient. furthermore, the isolate revealed to grow only around the x-factor impregnated discs when examined using the x and v disc strips. however, not only rapid-nh system but also the vitek2 system failed to identify the isola ... | 2017 | 28673558 |
| immune modulating topical s100a8/a9 inhibits growth of pseudomonas aeruginosa and mitigates biofilm infection in chronic wounds. | pseudomonas aeruginosa biofilm maintains and perturbs local host defense, hindering timely wound healing. previously, we showed that p. aeruginosa suppressed s100a8/a9 of the murine innate host defense. we assessed the potential antimicrobial effect of s100a8/a9 on biofilm-infected wounds in a murine model and p. aeruginosa growth in vitro. seventy-six mice, inflicted with a full-thickness burn wound were challenged subcutaneously (s.c.) by 10⁶ colony-forming units (cfus) of p. aeruginosa biofil ... | 2017 | 28672877 |
| individual and combined effects of engineered peptides and antibiotics on pseudomonas aeruginosa biofilms. | pseudomonas aeruginosa is involved in a variety of difficult-to-treat infections frequently due to biofilm formation. to identify useful antibiofilm strategies, this article evaluated efficacy of two newly engineered cationic antimicrobial peptides (17biphe2 and dasamp2), traditional antibiotics, and their combinations against biofilms at different stages. 17biphe2 is designed based on the 3d structure of human cathelicidin ll-37 and dasamp2 is derived from database screening. while both peptide ... | 2017 | 28672834 |
| the distribution of carbapenem- and colistin-resistance in gram-negative bacteria from the tamil nadu region in india. | the occurrence of carbapenem- and colistin-resistance among gram-negative bacteria is increasing worldwide. the aim of this study was to understand the distribution of carbapenem- and colistin-resistance in two areas in tamil nadu, india. | 2017 | 28671537 |
| chemical composition, antioxidant properties, α-glucosidase inhibitory and antimicrobial activity of essential oils from acacia mollissima and acacia cyclops cultivated in tunisia. | the genus acacia is quite large and can be found in the warm subarid and arid parts but little is known about its chemistry, specially the volatile parts. the volatile oils from fresh flowers of a. mollissima and a. cyclops (growing in tunisia) obtained by hydrodistillation were analyzed by gc then gc-ms. 18 (94.7% of the total oil composition) and 23 (97.4%) compounds were identified in these oils, respectively. (e,e)-α-farnesene (51.5%) and (e)-cinnamyl alcohol (10.7%) constituted the major co ... | 2017 | 28670852 |
| multilocus sequence typing of carbapenem resistant pseudomonas aeruginosa isolates from patients presenting at port elizabeth hospitals, south africa. | pseudomonas aeruginosa is an important nosocomial pathogen that exhibits multiple drug resistance with increasing frequency, especially to carbapenems making patient treatment difficult. carbapenem-resistance may be caused by porin gene mutations, active drug efflux, and carbapenemase production. this study evaluated the incidence of genes responsible for carbapenemase production in carbapenem-resistant pseudomonas aeruginosa and assessed the genetic relatedness of the isolates by multi locus se ... | 2017 | 28670642 |
| extended-spectrum beta-lactamase producing gram negative bacteria in iran: a review. | the emergence and spread of extended spectrum β-lactamase (esbl)-producing gram- negative bacteria (gnb), particularly in enterobacteriaceae, acinetobacter baumannii, and pseudomonas aeruginosa, have increased all over the world. esbls are characterized by their ability to hydrolyze β-lactams, early cephalosporins, oxyimino-thiazolyl cephalosporins, and monobactams, but not cephamycins or carbapenems. the rate of nosocomial infections caused by esbl-producing gnb in asia pacific has increased an ... | 2017 | 28670639 |
| inhaled colistin monotherapy for respiratory tract infections in adults without cystic fibrosis: a systematic review and meta-analysis. | inhaled colistin is becoming increasingly popular against respiratory tract infections due to multidrug resistant (mdr) gram-negative bacteria since it may overcome the problems associated with iv administration. | 2017 | 28669836 |
| bactericidal activity of cyclotides where phosphatidylethanolamine-lipid selectivity determines antimicrobial spectra. | cyclotides are a family of plant peptides characterized by a cystine knot embedded in a macrocyclic backbone. they bind to and disrupt phospholipid membranes, which explain their lytic activity on cells. in this study, we expose the full antibacterial potency of cyclotides by avoiding its inhibition by rich growth media assay conditions. for that purpose a two-step microdilution assay protocol was developed, using non-growing conditions during initial peptide incubation. a diverse set of cycloti ... | 2017 | 28669767 |
| detection of blavim-7 in an extensively drug-resistant pseudomonas aeruginosa isolate belonging to st1284 in brazil. | we described for the first time an extensively drug-resistant pseudomonas aeruginosa isolate belonging to st1284 carrying a plasmid-mediated blavim-7 in brazil. the blavim-7 was harbored by an integron that also carried aaca4 and blaoxa-46. multiple virulence factors were also detected. | 2017 | 28669677 |
| nationwide surveillance of bacterial respiratory pathogens conducted by the surveillance committee of japanese society of chemotherapy, the japanese association for infectious diseases, and the japanese society for clinical microbiology in 2012: general view of the pathogens' antibacterial susceptibility. | the nationwide surveillance on antimicrobial susceptibility of bacterial respiratory pathogens from the patients in japan was conducted by japanese society of chemotherapy, japanese association for infectious diseases and japanese society for clinical microbiology in 2012. the isolates were collected from clinical specimens obtained from well-diagnosed adult patients with respiratory tract infections during the period between january and december in 2012 by three societies. antimicrobial suscept ... | 2017 | 28669567 |
| efficient targeting of cd13 on cancer cells by the immunotoxin scfv13-eta' and the bispecific scfv [13xds16]. | treatment of cancer using standard chemotherapy still offers a poor prognosis combined with severe side effects. novel antibody-based therapies have been shown to overcome low efficiency and lack of selectivity by targeting cancer-associated antigens, such as aminopeptidase cd13. | 2017 | 28669053 |
| co-infection of pseudomonas aeruginosa and stenotrophomonas maltophilia in hospitalised pneumonia patients has a synergic and significant impact on clinical outcomes. | ventilator-acquired pneumonia and hospital community-acquired pneumonia are frequently caused by gram-negative and -positive bacteria. we noted that pneumonia patients with co-infection of pseudomonas aeruginosa and stenotrophomonas maltophilia had a poor clinical outcome. to verify this, we retrospectively reviewed pneumonia cases at hebei general hospital from 2010 to 2015. these cases were grouped into four categories: (1) co-infection with p. aeruginosa and s. maltophilia, (2) infection with ... | 2017 | 28669016 |
| antimicrobial activities of lcb10-0200, a novel siderophore cephalosporin, against the clinical isolates of pseudomonas aeruginosa and other pathogens. | infections caused by multidrug-resistant bacteria, including pseudomonas aeruginosa, are threating public health worldwide. therefore, a novel antibacterial agent is needed for treating these infections. here, we investigated the in vitro and in vivo activities of a novel siderophore-conjugated cephalosporin lcb10-0200 against the clinical isolates of gram-negative baeria, including multidrug-resistant p. aeruginosa. in vitro susceptibility to lcb10-0200 was assessed by performing a two-fold aga ... | 2017 | 28668680 |
| successful treatment of pseudomonas aeruginosa osteomyelitis with antibiotic monotherapy of limited duration. | the aim of this study was to present a 15-year experience and provide a comprehensive analysis of a large cohort of patients with pseudomonas aeruginosa osteomyelitis. | 2017 | 28668598 |
| defining lipoprotein localisation by fluorescence microscopy. | in recent years it has become evident that lipoproteins play crucial roles in the assembly of bacterial envelope-embedded nanomachineries and in the processes of protein export/secretion. in this chapter we describe a method to determine their precise localisation, for example inner versus outer membrane, in gram-negative bacteria using human opportunistic pathogen pseudomonas aeruginosa as a model. a fusion protein between a given putative lipoprotein and the red fluorescent protein mcherry mus ... | 2017 | 28667602 |
| post-secretional activation of protease iv by quorum sensing in pseudomonas aeruginosa. | protease iv (piv), a key virulence factor of pseudomonas aeruginosa is a secreted lysyl-endopeptidase whose expression is induced by quorum sensing (qs). we found that piv expressed in qs mutant has severe reduction of activity in culture supernatant (cs), even though it is overexpressed to high level. piv purified from the qs mutant (m-piv) had much lower activity than the piv purified from wild type (p-piv). we found that the propeptide cleaved from prepro-piv was co-purified with m-piv, but n ... | 2017 | 28667333 |
| synthesis and characterization of zro2 nanoparticles-antimicrobial activity and their prospective role in dental care. | nanomaterials are exerting a pull on deal with biological and pharmaceutical applications. biomedical grade of zirconia reveals potential mechanical features of oxide ceramics. in this study, antimicrobial activity and anti-tooth decay applications of the synthesized nps of zro2 were determined. the as-prepared zro2 nps were characterized by uv-vis spectroscopy, ftir and xrd, which determined the formation of zro2nps and their crystalline nature. sem analysis further revealed spherical shaped np ... | 2017 | 28666841 |
| anesthetic agents, neurodevelopmental risk and the connection to bacterial infections. | this short communication identifies a significant flaw in research investigating the neurodevelopmental consequences of general anesthesia exposure. we have identified that chronic environmental exposure to pervasive air pollutants that are also widely used as anesthetic agents, specifically nitrous oxide (n2o), may contribute to the rising prevalence of neurodevelopmental disorders. consistent with the emerging link between microbes and psychiatric illness risk, this epidemiological analysis ex ... | 2017 | 28666807 |
| activity of innate antimicrobial peptides and ivacaftor against clinical cystic fibrosis respiratory pathogens. | there is a clear need for new antimicrobials to improve current treatment of chronic lung infection in people with cystic fibrosis (cf). this study determined the activities of antimicrobial peptides (amps) and ivacaftor, a novel cf transmembrane conductance regulator potentiator, for cf treatment. antimicrobial activities of amps [ll37, human β-defensins (hβd) 1-4 and slpi] and ivacaftor against clinical respiratory isolates (pseudomonas aeruginosa, staphylococcus aureus, streptococcus spp., ac ... | 2017 | 28666755 |
| the papi-1 pathogenicity island-encoded small rna pesa influences pseudomonas aeruginosa virulence and modulates pyocin s3 production. | small non-coding rnas (srnas) are post-transcriptional regulators of gene expression that have been recognized as key contributors to bacterial virulence and pathogenic mechanisms. in this study, we characterized the srna pesa of the opportunistic human pathogen pseudomonas aeruginosa. we show that pesa, which is transcribed within the pathogenicity island papi-1 of p. aeruginosa strain pa14, contributes to p. aeruginosa pa14 virulence. in fact, pesa gene deletion resulted in a less pathogenic s ... | 2017 | 28665976 |
| prophylactic use of intraoperative vancomycin powder and postoperative infection: an analysis of microbiological patterns in 1200 consecutive surgical cases. | objective wound infections following spinal surgery for deformity place a high toll on patients, providers, and the health care system. the prophylactic application of intraoperative vancomycin powder has been shown to lower the infection risk after thoracolumbar decompression and fusion for deformity correction. the purpose of this study was to assess the microbiological patterns of postoperative surgical site infections (ssis) after prophylactic use of vancomycin powder in adult patients under ... | 2017 | 28665245 |
| the influence of agitation on oil substrate dispersion and oxygen transfer in pseudomonas aeruginosa usm-ar2 fermentation producing rhamnolipid in a stirred tank bioreactor. | water-immiscible substrate, diesel, was supplied as the main substrate in the fermentation of pseudomonas aeruginosa usm-ar2 producing rhamnolipid biosurfactant, in a stirred tank bioreactor. in addition to the typical gas-aqueous system, this system includes gas-hydrocarbon-aqueous phases and the presence of surfactant (rhamnolipid) in the fermentation broth. the effect of diesel dispersion on volumetric oxygen transfer coefficient, k l a, and thus oxygen transfer, was evaluated at different ag ... | 2017 | 28664380 |
| emergence of antimicrobial resistance to pseudomonas aeruginosa in the intensive care unit: association with the duration of antibiotic exposure and mode of administration. | antibiotics are frequently used in intensive care units (icus), and their use is associated with the emergence of bacterial resistance to antibiotics. the aim of this study was to investigate the association between the emergence of pseudomonas aeruginosa resistance and the duration of antibiotic exposure or mode of administration in an icu unit. | 2017 | 28664350 |
| antimicrobial and cytotoxic evaluation of some herbal essential oils in comparison with common antibiotics in bioassay condition. | since ancient times, various infectious diseases have been treated using herbal drugs. today, efforts regarding the discovery of the effectual components of plants possessing antimicrobial properties are advanced. herbal essential oils are widely used for treatment of various diseases, and they play an important role in health care considerations. | 2014 | 28664090 |
| monitoring in vitro antibacterial efficacy of 26 indian spices against multidrug resistant urinary tract infecting bacteria. | to screen methanolic extracts of 26 commonly used indian spices against nine species of uropathogenic bacteria (enterococcus faecalis, staphylococcus aureus, acinetobacter baumannii, citrobacter freundii, enterobacter aerogenes, escherichia coli, klebsiella pneumoniae, proteus mirabilis, and pseudomonas aeruginosa), isolated from clinical samples of a tertiary care hospital for antibacterial activity. | 2014 | 28664089 |
| bioconversion of shrimp waste penaeus merguiensis using lactic acid fermentation: an alternative procedure for chemical extraction of chitin and chitosan. | chitin extraction from shrimp wastes by biological treatment, using the pseudomonas aeruginosa was a positive and simple method. in order to look for the optimal conditions, the wastes were incubated at 30°c and 100rpm in different glucose (0%, 10%, 15% and 20%) and inoculation (10%, 15% and 20%) concentrations for 4 and 6days. at the end of fermentation, protease activity was investigated at different temperatures and temperature 50°c was considered as the optimum. the results obtained also sho ... | 2017 | 28663153 |
| three new abietane-type diterpenoids from plectranthus africanus and their antibacterial activities. | three new abietane-type diterpenoids, plectranthroyleanones a - c (1 - 3), together with five known compounds (4 - 8) were isolated from the methanol extract of the whole plant of plectranthus africanus using column chromatography techniques. the structures of the new compounds were elucidated using a combination of 1d and 2d nmr and hresims analyses. compound 1 exhibited weak activities with minimal inhibitory concentration values of 75 µg/ml against gram-positive bacteria, bacillus subtilis an ... | 2017 | 28662528 |
| activating transcription factor 3 protects mice against pseudomonas aeruginosa-induced acute lung injury by interacting with lipopolysaccharide binding protein. | excessive inflammatory response is critical event in the pathogenesis of acute lung injury (ali). previous study has shown that activating transcription factor 3 (atf3) plays a role in downregulate inflammatory responses including ventilation-induced ali. we hypothesized that atf3 have a protective effect in ali induced by pseudomonas aeruginosa. pa was intra-tracheally administrated to atf3 knock-out (ko) mice to establish ali model. inflammatory factors, balf protein, lung wet to dry ratio, lu ... | 2017 | 28662411 |
| innovative non-thermal plasma disinfection process inside sealed bags: assessment of bactericidal and sporicidal effectiveness in regard to current sterilization norms. | in this work, we developed a device capable to generate a non-thermal plasma discharge inside a sealed bag. the aim of this study was to assess the effectiveness of the oxygen, nitrogen and argon plasma sterilization on pseudomonas aeruginosa, staphylococcus aureus and bacillus subtilis spores according to the nf en 556 norm. moreover the bag integrity which is a critical key to maintain the sterile state of items after the end of the process was verified by fourier transform infrared (ftir) and ... | 2017 | 28662202 |
| quantitative proteomics analysis of proteins involved in alkane uptake comparing the profiling of pseudomonas aeruginosa sjtd-1 in response to n-octadecane and n-hexadecane. | while many data are available on genes encoding proteins for degradation of hydrocarbons in bacteria, the impact of alkane on transporter protein expression is unclear. pseudomonas aeruginosa sjtd-1 is a strain that can consume medium- and long-chain n-alkanes. in order to study the proteins involved in n-octadecane uptake, we use itraq and label free comparative proteomics analysis to identify the proteins of alkane uptake in response to n-octadecane (c18) comparing with n-hexadecane (c16) in p ... | 2017 | 28662172 |
| a conserved mitochondrial surveillance pathway is required for defense against pseudomonas aeruginosa. | all living organisms exist in a precarious state of homeostasis that requires constant maintenance. a wide variety of stresses, including hypoxia, heat, and infection by pathogens perpetually threaten to imbalance this state. organisms use a battery of defenses to mitigate damage and restore normal function. previously, we described a caenorhabditis elegans-pseudomonas aeruginosa assay (liquid killing) in which toxicity to the host is dependent upon the secreted bacterial siderophore pyoverdine. ... | 2017 | 28662060 |
| phenolic compounds in antimicrobial therapy. | long-term treatment with several conventional antibiotics can cause harmful side effects that can be alleviated by antioxidant therapy. phenolic compounds (pcs) are the best source of antioxidants, and to identify the most suitable polyphenols for use as a supportive supplement during antibiotic therapy, this study screened a series of pcs to establish their antibacterial potential, including their biofilm and β-lactamase inhibition activity. several pcs were tested for antibacterial activity ag ... | 2017 | 28661772 |
| single-step capture and targeted metabolomics of alkyl-quinolones in outer membrane vesicles of pseudomonas aeruginosa. | outer membrane vesicles (omvs) are secreted by all gram-ve pathogens. these nano-scale delivery vehicles contain discrete arrays of prokaryotic pathogenic determinants, including a family of low molecular weight (mw) lipidic quorum signaling alkyl-quinolones (aqs). these are synthesized from β-keto-fatty acids and function like primordial lipidic hormones, which regulate numerous pathogenic factors both inter-species and intra-species. significantly, aqs can also directly exacerbate pathogenesis ... | 2017 | 28660582 |
| a new pseudomonas quinolone signal (pqs) binding partner: mexg. | the opportunistic pathogen pseudomonas aeruginosa utilises the cell-cell signalling mechanism known as quorum sensing to regulate virulence. p. aeruginosa produces two quinolone-based quorum sensing signalling molecules; the pseudomonas quinolone signal (pqs) and its biosynthetic precursor 2-heptyl-4(1h)-quinolone (hhq). to date, only one receptor (the pqsr protein) has been identified that is capable of binding pqs and hhq. here, we report on the synthesis of pqs and hhq affinity probes for che ... | 2016 | 28660026 |
| convexity subarachnoid hemorrhage, pseudomonas aeruginosa (pa) infective endocarditis and left atrial appendage occluder (laao) device infection. a case report. | an 83 year-old-man with left atrial appendage occluder (laao) developed pseudomonas aeruginosa (pa) infective endocarditis. mri at day 3 of onset showed distal small infarcts in both middle cerebral arteries and left postero-inferior cerebellar artery territories. mri at day 6 revealed two sites of convexity subarachnoid hemorrhage (csah). mra and cta failed to reveal a mycotic aneurysm. the radiologic findings favor the assumption of necrosis of distal branches of mca or of pial arteries wall. ... | 2017 | 28660006 |
| the effect of phenazine-1-carboxylic acid on mycelial growth of botrytis cinerea produced by pseudomonas aeruginosa lv strain. | one of the most important postharvest plant pathogens that affect strawberries, grapes and tomatoes is botrytis cinerea, known as gray mold. the fungus remains in latent form until spore germination conditions are good, making infection control difficult, causing great losses in the whole production chain. this study aimed to purify and identify phenazine-1-carboxylic acid (pca) produced by the pseudomonas aeruginosa lv strain and to determine its antifungal activity against b. cinerea. the comp ... | 2017 | 28659907 |
| pulmonary pseudomonas aeruginosa infection induces autophagy and proteasome proteolytic pathways in skeletal muscles: effects of a pressurized whey protein-based diet in mice. | background: pulmonary pseudomonas aeruginosa infection in cystic fibrosis patients is associated with skeletal muscle atrophy. in this study, we investigated the effects of p. aeurginosa infection and a whey protein-rich diet on skeletal muscle proteolytic pathways. design: an agar bead model of pulmonary p. aeurginosa infection was established in adult c57/bl6 mice. protein ubiquitinaiton, lipidation of lc3b protein and expressions of autophagy-related genes and ubiquitin e3 ligases were quanti ... | 2017 | 28659735 |
| bacterial genus is a risk factor for major amputation in patients with diabetic foot. | to evaluate whether bacterial genus is a risk factor for major amputation in patients with diabetic foot and infected ulcer. | 2017 | 28658333 |
| prevalence of esbl-producing pseudomonas aeruginosa isolates in warsaw, poland, detected by various phenotypic and genotypic methods. | knowledge of the prevalence of esbl enzymes among p. aeruginosa strains compared to the enterobacteraiceae family is limited. the phenotypic tests recommended by eucast for the detection of esbl-producing enterobacteriaceae are not always suited for p. aeruginosa strains. this is mainly due to the presence of other families of esbls in p. aeruginosa isolates more often than in enterobacteriaceae, production of natural ampc cephalosporinase and its overexpression, and co-production of metallo-β-l ... | 2017 | 28658322 |
| corticosteroid-resistant inflammatory signalling in pseudomonas-infected bronchial cells. | decreasing the inflammatory response that leads to tissue damage during cystic fibrosis (cf) lung disease has been a long-standing goal of cf therapy. while corticosteroids are widely used anti-inflammatory drugs, their efficacy in cf lung disease remains debated. the complex interaction between the colonising bacteria and the host environment may impact corticosteroid responsiveness. in this study, sputum samples from adult cf patients were collected at baseline and during pulmonary exacerbatio ... | 2017 | 28656134 |
| one-step purification and characterization of alginate lyase from a clinical pseudomonas aeruginosa with destructive activity on bacterial biofilm. | pseudomonas aeruginosa is a gram-negative and aerobic rod bacterium that displays mucoid and non-mucoid phenotype. mucoid strains secrete alginate, which is the main agent of biofilms in chronic p. aeruginosa infections, show high resistance to antibiotics; consequently, the biological disruption of mucoid p. aeruginosa biofilms is an attractive area of study for researchers. alginate lyase gene (algl) is a member of alginate producing operon which by glycosidase activity produces primer for oth ... | 2017 | 28656080 |