Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| the probiotic butyricicoccus pullicaecorum reduces feed conversion and protects from potentially harmful intestinal microorganisms and necrotic enteritis in broilers. | probiotics which do not result in the development and spread of microbial resistance are among the candidate replacements for antibiotics previously used as growth promotors. in this study the effect of in-feed supplementation of the butyrate producing butyricicoccus pullicaecorum strain 25-3(t) on performance, intestinal microbiota and prevention of necrotic enteritis (ne), a disease caused by clostridium perfringens was evaluated in broilers. for the performance study, day old ross 308 chicks ... | 2016 | 27708624 |
| antibacterial activities of wasabi against escherichia coli o157:h7 and staphylococcus aureus. | escherichia coli o157:h7 and staphylococcus aureus are two of the major pathogens frequently involved in foodborne outbreaks. control of these pathogens in foods is essential to food safety. it is of great interest in the use of natural antimicrobial compounds present in edible plants to control foodborne pathogens as consumers prefer more natural "green" foods. allyl isothiocyanate (aitc) is an antimicrobial compound naturally present in wasabi (japanese horseradish) and several other edible pl ... | 2016 | 27708622 |
| beneficial effects of spices in food preservation and safety. | spices have been used since ancient times. although they have been employed mainly as flavoring and coloring agents, their role in food safety and preservation have also been studied in vitro and in vivo. spices have exhibited numerous health benefits in preventing and treating a wide variety of diseases such as cancer, aging, metabolic, neurological, cardiovascular, and inflammatory diseases. the present review aims to provide a comprehensive summary of the most relevant and recent findings on ... | 2016 | 27708620 |
| zika: the origin and spread of a mosquito-borne virus. | to describe the temporal and geographical distribution of zika virus infection and associated neurological disorders, from 1947 to 1 february 2016, when zika became a public health emergency of international concern (pheic). | 2016 | 27708473 |
| universal microbial diagnostics using random dna probes. | early identification of pathogens is essential for limiting development of therapy-resistant pathogens and mitigating infectious disease outbreaks. most bacterial detection schemes use target-specific probes to differentiate pathogen species, creating time and cost inefficiencies in identifying newly discovered organisms. we present a novel universal microbial diagnostics (umd) platform to screen for microbial organisms in an infectious sample, using a small number of random dna probes that are ... | 2016 | 27704040 |
| a rare case of guillain-barré syndrome presenting with abdominal pain. | guillain-barré syndrome (gbs) is a heterogeneous condition that encompasses acute immune-mediated polyneuropathies. gbs is the most frequent cause of acute neuromuscular paralysis worldwide and constitutes one of the most serious emergencies in neurology. description of case: as it presents extremely rarely with the first symptom being abdominal pain, herein we report the case of a 48-year-old man who presented with acute abdominal pain and diagnosed with gbs. the patient required mechanical ven ... | 2015 | 27703314 |
| role of glycosyltransferases modifying type b flagellin of emerging hypervirulent clostridium difficile lineages and their impact on motility and biofilm formation. | clostridium difficile is the principal cause of nosocomial infectious diarrhea worldwide. the pathogen modifies its flagellin with either a type a or type b o-linked glycosylation system, which has a contributory role in pathogenesis. we study the functional role of glycosyltransferases modifying type b flagellin in the 023 and 027 hypervirulent c. difficile lineages by mutagenesis of five putative glycosyltransferases and biosynthetic genes. we reveal their roles in the biosynthesis of the flag ... | 2016 | 27703012 |
| distribution of campylobacter jejuni multilocus sequence types isolated from chickens in poland. | poultry is recognized as the most important source of food-related transmission of campylobacter jejuni to humans and campylobacteriosis is the most commonly reported zoonotic bacterial disease in the european union. it has been documented that c. jejuni is genetically diverse and analyses of bacterial isolates usually show a large strain variety. therefore, molecular typing of strains represents an important tool to study the genetic diversity of isolates and to trace individual strains that ca ... | 2016 | 27702925 |
| fatal campylobacter jejuni ileocolitis. | campylobacter infection usually starts in the jejunum and ileum and progresses distally. the case fatality rate is low and most occur in elderly or patients with comorbidity as in this case. antibiotics should be used in severe cases or patients at risk. the choices are macrolides and fluoroquinolones. however, in some countries quinolone resistance is increasing, as in spain. we shouldn´t forget this fact for the proper treatment approach and specifically in refractory cases. | 2016 | 27701889 |
| the influence of dissolved oxygen level and medium on biofilm formation by campylobacter jejuni. | campylobacter jejuni survival in aerobic environments has been suggested to be mediated by biofilm formation. biofilm formation by eight c. jejuni strains under both aerobic and microaerobic conditions in different broths (mueller-hinton (mh), bolton and brucella) was quantified. the dissolved oxygen (do) content of the broths under both incubation atmospheres was determined. biofilm formation for all strains was highest in mh broth under both incubation atmospheres. four strains had lower biofi ... | 2017 | 27697161 |
| tlr4 and tlr21 expression, mif, ifn-β, md-2, cd14 activation, and siga production in chickens administered with efal41 strain challenged with campylobacter jejuni. | the protective effect of enterococcus faecium efal41 on chicken's caecum in relation to the tlr (tlr4 and tlr21) activation and production of luminal iga challenged with campylobacter jejuni ccm6191 was assessed. the activation of mif, ifn-β, md-2 and cd14 was followed-up after bacterial infection. day-old chicks (40) were divided into four groups (n = 10): control (c), e. faecium al41 (efal41), c. jejuni (cj) and combined e. faecium al41+c. jejuni (efal41+cj). relative mrna expression of tlr4, ... | 2017 | 27696326 |
| momp from campylobacter jejuni is a trimer of 18-stranded β-barrel monomers with a ca(2+) ion bound at the constriction zone. | the gram-negative organism campylobacter jejuni is the major cause of food poisoning. unlike escherichia coli, which has two major porins, ompc and ompf, c. jejuni has one, termed major outer membrane protein (momp) through which nutrients and antibiotics transit. we report the 2.1-å crystal structure of c. jejuni momp expressed in e. coli and a lower resolution but otherwise identical structure purified directly from c. jejuni. the 2.1-å resolution structure of recombinant momp showed that alth ... | 2016 | 27693650 |
| colorimetric integrated pcr protocol for rapid detection of vibrio parahaemolyticus. | rapid detection of pathogens is of great significance for food safety and disease diagnosis. a new colorimetric method for rapid and easy detection of vibrio parahaemolyticus (v. parahaemolyticus or vp) has been developed in this research. a specific sequence was designed and integrated with the forward primer for molecular detection of vp. this specific sequence was tested and treated as the horseradish peroxidase (hrp)-mimicking dnazyme and could be amplified during the polymerase chain reacti ... | 2016 | 27690041 |
| multilocus sequence typing of campylobacter concisus from danish diarrheic patients. | the emerging enteric pathogen campylobacter concisus is associated with prolonged diarrhea and inflammatory bowel disease. previous studies have shown that c. concisus strains are very genetically diverse. nevertheless, c. concisus strains have been divided into two genomospecies, where gs1 strains have been isolated predominantly from healthy individuals, while the gs2 cluster consists of isolates primarily from diarrheic individuals. the aim of the present study was to determine the genetic di ... | 2016 | 27688814 |
| complete genome sequences of campylobacter jejuni strains od267 and wp2202 isolated from retail chicken livers and gizzards reveal the presence of novel 116-kilobase and 119-kilobase megaplasmids with type vi secretion systems. | genome sequences of campylobacter jejuni strains od267 and wp2202, isolated from chicken livers and gizzards, showed the presence of novel 116-kb and 119-kb megaplasmids, respectively. the two megaplasmids carry a type vi secretion system and tetracycline resistance genes. these are the largest sequenced campylobacter plasmids to date. | 2016 | 27688318 |
| evaluation of bacteriocinogenic activity, safety traits and biotechnological potential of fecal lactic acid bacteria (lab), isolated from griffon vultures (gyps fulvus subsp. fulvus). | lactic acid bacteria (lab) are part of the gut microbiota and produce ribosomally synthesized antimicrobial peptides or bacteriocins with interest as natural food preservatives and therapeutic agents. bacteriocin-producing lab are also attractive as probiotics. griffon vultures (gyps fulvus subspecies fulvus) are scavenger birds that feed almost exclusively on carrion without suffering apparent ill effects. therefore, griffon vultures might be considered a reservoir of bacteriocin-producing lact ... | 2016 | 27688001 |
| developing a salivary antibody multiplex immunoassay to measure human exposure to environmental pathogens. | the etiology and impacts of human exposure to environmental pathogens are of major concern worldwide and, thus, the ability to assess exposure and infections using cost effective, high-throughput approaches would be indispensable. this manuscript describes the development and analysis of a bead-based multiplex immunoassay capable of measuring the presence of antibodies in human saliva to multiple pathogens simultaneously. saliva is particularly attractive in this application because it is noninv ... | 2016 | 27685162 |
| the science behind the probiotic strain bifidobacterium animalis subsp. lactis bb-12(®). | this review presents selected data on the probiotic strain bifidobacterium animalis subsp. lactis bb-12(®) (bb-12(®)), which is the world's most documented probiotic bifidobacterium. it is described in more than 300 scientific publications out of which more than 130 are publications of human clinical studies. the complete genome sequence of bb-12(®) has been determined and published. bb-12(®) originates from chr. hansen's collection of dairy cultures and has high stability in foods and as freeze ... | 2014 | 27682233 |
| bacterial multidrug efflux pumps: much more than antibiotic resistance determinants. | bacterial multidrug efflux pumps are antibiotic resistance determinants present in all microorganisms. with few exceptions, they are chromosomally encoded and present a conserved organization both at the genetic and at the protein levels. in addition, most, if not all, strains of a given bacterial species present the same chromosomally-encoded efflux pumps. altogether this indicates that multidrug efflux pumps are ancient elements encoded in bacterial genomes long before the recent use of antibi ... | 2016 | 27681908 |
| non food-related risk factors of campylobacteriosis in canada: a matched case-control study. | campylobacteriosis is a prominent bacterial gastrointestinal infection worldwide with several transmission pathways. its non-foodborne routes have been less documented and quantified. the study aimed to quantitatively explore the role of potential risk factors not directly associated with food for sporadic cases of c. jejuni infection in canada. | 2016 | 27677338 |
| two glycosyltransferase genes of haemophilus parasuis sc096 implicated in lipooligosaccharide biosynthesis, serum resistance, adherence, and invasion. | haemophilus parasuis is a common opportunistic pathogen known for its ability to colonize healthy piglets and causes glässer's disease. the lipooligosaccharide (los) of h. parasuis is a potential virulence-associated factor. in this study, two putative glycosyltransferases that might be involved in los synthesis in h. parasuis sc096 were identified (lgtb and lex-1). mutants were constructed to investigate the roles of the lgtb and lex-1 genes. the los from the δlgtb or δlex-1 mutant showed trunc ... | 2016 | 27672622 |
| quantification of growth of campylobacter and extended spectrum β-lactamase producing bacteria sheds light on black box of enrichment procedures. | campylobacter is well recognized as the leading cause of bacterial foodborne diarrheal disease worldwide, and is routinely found in meat originating from poultry, sheep, pigs, and cattle. effective monitoring of campylobacter contamination is dependent on the availability of reliable detection methods. the method of the international organization for standardization for the detection of campylobacter spp. in food (iso 10272-1:2006) recommends the use of bolton broth (bb) as selective enrichment ... | 2016 | 27672384 |
| polyphosphate and associated enzymes as global regulators of stress response and virulence in campylobacter jejuni. | campylobacter jejuni (c. jejuni), a gram-negative microaerophilic bacterium, is a predominant cause of bacterial foodborne gastroenteritis in humans worldwide. despite its importance as a major foodborne pathogen, our understanding of the molecular mechanisms underlying c. jejuni stress survival and pathogenesis is limited. inorganic polyphosphate (poly p) has been shown to play significant roles in bacterial resistance to stress and virulence in many pathogenic bacteria. c. jejuni contains the ... | 2016 | 27672264 |
| crispr-cas: biology, mechanisms and relevance. | prokaryotes have evolved several defence mechanisms to protect themselves from viral predators. clustered regularly interspaced short palindromic repeats (crispr) and their associated proteins (cas) display a prokaryotic adaptive immune system that memorizes previous infections by integrating short sequences of invading genomes-termed spacers-into the crispr locus. the spacers interspaced with repeats are expressed as small guide crispr rnas (crrnas) that are employed by cas proteins to target i ... | 2016 | 27672148 |
| the host-pathogen interaction in campylobacter jejuni infection of chickens: an understudied aspect that is crucial for effective control. | 2016 | 27668455 | |
| bacterial flagellar capping proteins adopt diverse oligomeric states. | flagella are crucial for bacterial motility and pathogenesis. the flagellar capping protein (flid) regulates filament assembly by chaperoning and sorting flagellin (flic) proteins after they traverse the hollow filament and exit the growing flagellum tip. in the absence of flid, flagella are not formed, resulting in impaired motility and infectivity. here, we report the 2.2 å resolution x-ray crystal structure of flid from pseudomonas aeruginosa, the first high-resolution structure of any flid p ... | 2016 | 27664419 |
| an approach for predicting essential genes using multiple homology mapping and machine learning algorithms. | investigation of essential genes is significant to comprehend the minimal gene sets of cell and discover potential drug targets. in this study, a novel approach based on multiple homology mapping and machine learning method was introduced to predict essential genes. we focused on 25 bacteria which have characterized essential genes. the predictions yielded the highest area under receiver operating characteristic (roc) curve (auc) of 0.9716 through tenfold cross-validation test. proper features w ... | 2016 | 27660763 |
| guillain-barré syndrome: what have we learnt during one century? a personal historical perspective. | we are approaching the centenary of the first description of guillain-barré syndrome. the past 30 years had witnessed an amazing progress in the understanding of the immunological and pathological mechanisms of this disorder. we now recognize that guillain-barré syndrome is remarkably heterogeneous and under this umbrella term are several variants and subtypes with distinct clinical, electrophysiological and immunopathological features. this review is a historical journey, through a personal per ... | 2016 | 27659900 |
| extraction of total nucleic acids from bacterial isolates using the biomérieux nuclisens easymag total nucleic acid extractor. | the biomerieux nuclisens easymag total nucleic acid extractor was evaluated for use on bacterial isolates in the clinical microbiology laboratory. forty eight isolates were extracted, yielding quantifiable amounts of dna for all isolates. the easymag is appropriate for dna extraction from bacterial isolates and will be incorporated in the clinical laboratory. | 2016 | 27658494 |
| ethyl pyruvate: an anti-microbial agent that selectively targets pathobionts and biofilms. | the microbiota has a strong influence on health and disease in humans. a causative shift favoring pathobionts is strongly linked to diseases. therefore, anti-microbial agents selectively targeting potential pathogens as well as their biofilms are urgently demanded. here we demonstrate the impact of ethyl pyruvate, so far known as ros scavenger and anti-inflammatory agent, on planktonic microbes and biofilms. ethyl pyruvate combats preferably the growth of pathobionts belonging to bacteria and fu ... | 2016 | 27658257 |
| validation of a high-throughput multiplex genetic detection system for helicobacter pylori identification, quantification, virulence, and resistance analysis. | helicobacter pylori (h. pylori) infection is closely related to various gastroduodenal diseases. virulence factors and bacterial load of h. pylori are associated with clinical outcomes, and drug-resistance severely impacts the clinical efficacy of eradication treatment. existing detection methods are low-throughput, time-consuming and labor intensive. therefore, a rapid and high-throughput method is needed for clinical diagnosis, treatment, and monitoring for h. pylori. high-throughput multiplex ... | 2016 | 27656172 |
| mortality in children with complicated severe acute malnutrition is related to intestinal and systemic inflammation: an observational cohort study. | diarrhea affects a large proportion of children with severe acute malnutrition (sam). however, its etiology and clinical consequences remain unclear. | 2016 | 27655441 |
| structure of the catalytic domain of the colistin resistance enzyme mcr-1. | due to the paucity of novel antibiotics, colistin has become a last resort antibiotic for treating multidrug resistant bacteria. colistin acts by binding the lipid a component of lipopolysaccharides and subsequently disrupting the bacterial membrane. the recently identified plasmid-encoded mcr-1 enzyme is the first transmissible colistin resistance determinant and is a cause for concern for the spread of this resistance trait. mcr-1 is a phosphoethanolamine transferase that catalyzes the additio ... | 2016 | 27655155 |
| zonula occludens toxins and their prophages in campylobacter species. | we previously showed that zonula occludens toxin (zot) encoded by campylobacter concisus zot (808t) gene has the potential to initiate inflammatory bowel disease. this zot protein caused prolonged intestinal epithelial barrier damage, induced intestinal epithelial and macrophage production of tumor necrosis factor-α and enhanced the responses of macrophages to other microbes. in order to understand the potential virulence of zot proteins in other campylobacter species, in this study we examined ... | 2016 | 27651834 |
| emergence of a potent multidrug efflux pump variant that enhances campylobacter resistance to multiple antibiotics. | bacterial antibiotic efflux pumps are key players in antibiotic resistance. although their role in conferring multidrug resistance is well documented, the emergence of "super" efflux pump variants that enhance bacterial resistance to multiple drugs has not been reported. here, we describe the emergence of a resistance-enhancing variant (named re-cmeabc) of the predominant efflux pump cmeabc in campylobacter, a major zoonotic pathogen whose resistance to antibiotics is considered a serious antibi ... | 2016 | 27651364 |
| chemical, physical and morphological properties of bacterial biofilms affect survival of encased campylobacter jejuni f38011 under aerobic stress. | campylobacter jejuni is a microaerophilic pathogen and leading cause of human gastroenteritis. the presence of c. jejuni encased in biofilms found in meat and poultry processing facilities may be the major strategy for its survival and dissemination in aerobic environment. in this study, staphylococcus aureus, salmonella enterica, or pseudomonas aeruginosa was mixed with c. jejuni f38011 as a culture to form dual-species biofilms. after 4days' exposure to aerobic stress, no viable c. jejuni cell ... | 2016 | 27648759 |
| the helical shape of campylobacter jejuni promotes in vivo pathogenesis by aiding transit through intestinal mucus and colonization of crypts. | campylobacter jejuni is a helix-shaped enteric bacterial pathogen and a common cause of gastroenteritis. we recently developed a mouse model for this human pathogen utilizing the sigirr-deficient mouse strain, which exhibits significant intestinal inflammation in response to intestinal c. jejuni infection. in the current study, this mouse model was used to define whether c. jejuni's characteristic helical shape plays a role in its ability to colonize and elicit inflammation in the mouse intestin ... | 2016 | 27647867 |
| gut microbiota-brain axis. | to systematically review the updated information about the gut microbiota-brain axis. | 2016 | 27647198 |
| [hemolytic-uremic syndrome (hus) in children at the university hospital center in dakar: about four cases]. | hemolytic-uremic syndrome (hus) is a common cause of organic acute renal failure (arf) in children. it is a progressive complication of acute gastroenteritis (age), especially caused by escherichia coli in children. this study aimed to describe the clinical, therapeutic and evolutionary aspects of this affection in four children. we collected four cases of hus. the average age was 10,5 months (5-15mois), exclusively boys. clinical examination revealed a hemolytic anemia (pallor and jaundice), ol ... | 2016 | 27642476 |
| role of enterotoxigenic bacteroides fragilis in children less than 5 years of age with diarrhea in tabriz, iran. | diarrhea is the most frequent health problem among children in developing countries. defining the etiology of acute diarrhea is critical to disease therapy and prevention. some anaerobic bacteria such as enterotoxigenic bacteroides fragilis (etbf) strains cause diarrheal disease by production of enterotoxin in children less than 5 years old. | 2016 | 27635209 |
| pangenome and immuno-proteomics analysis of acinetobacter baumannii strains revealed the core peptide vaccine targets. | acinetobacter baumannii has emerged as a significant nosocomial pathogen during the last few years, exhibiting resistance to almost all major classes of antibiotics. alternative treatment options such as vaccines tend to be most promising and cost effective approaches against this resistant pathogen. in the current study, we have explored the pan-genome of a. baumannii followed by immune-proteomics and reverse vaccinology approaches to identify potential core vaccine targets. | 2016 | 27634541 |
| validation of the ansr® for campylobacter method for detection of thermophilic campylobacter spp. in chicken carcass rinse and turkey sponge samples. | a performance validation of the ansr® for campylobacter method was conducted in selected matrixes. this assay used selective nicking enzyme amplification technology to amplify target genes. samples were enriched for 20 to 24 h and then lysed. the assay was completed within 50 min using real-time detection in a combination incubator/fluorescence detector and software. when 50 distinct strains of campylobacter jejuni, c. lari, or c. coli were tested for inclusivity, all 50 strains produced positiv ... | 2016 | 27634328 |
| transfer of maternal antimicrobial immunity to hiv-exposed uninfected newborns. | the transfer of maternal immune factors to the newborn is critical for protection from infectious disease in early life. maternally acquired passive immunity provides protection until the infant is beyond early life's increased susceptibility to severe infections or until active immunity is achieved following infant's primary immunization. however, as reviewed here, human immunodeficiency virus (hiv) infection alters the transfer of immune factors from hiv-infected mothers to the hiv-exposed new ... | 2016 | 27630640 |
| the human milk oligosaccharide 2'-fucosyllactose quenches campylobacter jejuni-induced inflammation in human epithelial cells hep-2 and ht-29 and in mouse intestinal mucosa. | campylobacter jejuni causes diarrhea worldwide; young children are most susceptible. binding of virulent c. jejuni to the intestinal mucosa is inhibited ex vivo by α1,2-fucosylated carbohydrate moieties, including human milk oligosaccharides (hmoss). | 2016 | 27629573 |
| efficient whole-cell biocatalyst for neu5ac production by manipulating synthetic, degradation and transmembrane pathways. | to develop a strategy for producing n-acetyl-d-neuraminic acid (neu5ac), which is often synthesized from exogenous n-acetylglucosamine (glcnac) and pyruvate, but without using pyruvate. | 2017 | 27627899 |
| a rapid and efficient luminescence-based method for assaying phosphoglycosyltransferase enzymes. | phosphoglycosyltransferases (pgts) are families of integral membrane proteins with intriguingly diverse architectures. these enzymes function to initiate many important biosynthetic pathways including those leading to peptidoglycan, n-linked glycoproteins and lipopolysaccharide o-antigen. in spite of tremendous efforts, characterization of these enzymes remains a challenge not only due to the inherent difficulties associated with the purification of integral membrane proteins but also due to the ... | 2016 | 27624811 |
| dna supercoiling regulates the motility of campylobacter jejuni and is altered by growth in the presence of chicken mucus. | campylobacter jejuni is the leading cause of bacterial gastroenteritis in humans, but relatively little is known about the global regulation of virulence factors during infection of chickens or humans. this study identified dna supercoiling as playing a key role in regulating motility and flagellar protein production and found that this supercoiling-controlled regulon is induced by growth in chicken mucus. a direct correlation was observed between motility and resting dna supercoiling levels in ... | 2016 | 27624126 |
| the influence of prior modes of growth, temperature, medium, and substrate surface on biofilm formation by antibiotic-resistant campylobacter jejuni. | campylobacter jejuni is one of the most common causes of bacterial gastrointestinal food-borne infection worldwide. it has been suggested that biofilm formation may play a role in survival of these bacteria in the environment. in this study, the influence of prior modes of growth (planktonic or sessile), temperatures (37 and 42 °c), and nutrient conditions (nutrient broth and mueller-hinton broth) on biofilm formation by eight c. jejuni strains with different antibiotic resistance profiles was e ... | 2016 | 27623781 |
| campylobacter jejuni bacteremia in a patient with acute lymphocytic leukemia. | campylobacter jejuni is a slender, motile, non-spore-forming, helical-shaped, gram-negative bacterium. it is one of the most common causes of human gastroenteritis in the world. the aim of this study was to present a patient with acute lymphocytic leukemia (all), who was infected with campylobacter jejuni. | 2016 | 27621914 |
| status, antimicrobial mechanism, and regulation of natural preservatives in livestock food systems. | this review discusses the status, antimicrobial mechanisms, application, and regulation of natural preservatives in livestock food systems. conventional preservatives are synthetic chemical substances including nitrates/nitrites, sulfites, sodium benzoate, propyl gallate, and potassium sorbate. the use of artificial preservatives is being reconsidered because of concerns relating to headache, allergies, and cancer. as the demand for biopreservation in food systems has increased, new natural anti ... | 2016 | 27621697 |
| transition metals and virulence in bacteria. | transition metals are required trace elements for all forms of life. due to their unique inorganic and redox properties, transition metals serve as cofactors for enzymes and other proteins. in bacterial pathogenesis, the vertebrate host represents a rich source of nutrient metals, and bacteria have evolved diverse metal acquisition strategies. host metal homeostasis changes dramatically in response to bacterial infections, including production of metal sequestering proteins and the bombardment o ... | 2016 | 27617971 |
| protective effect of carnobacterium spp. against listeria monocytogenes during host cell invasion using in vitro ht29 model. | the pathogenesis of listeriosis results mainly from the ability of listeria monocytogenes to attach, invade, replicate and survive within various cell types in mammalian tissues. in this work, the effect of two bacteriocin-producing carnobacterium (c. divergens v41 and c. maltaromaticum v1) and three non-bacteriocinogenic strains: (c. divergens v41c9, c. divergens 2763, and c. maltaromaticum 2762) was investigated on the reduction of l. monocytogenes scott a plaque-forming during human infection ... | 2016 | 27617232 |
| an improved culture method for selective isolation of campylobacter jejuni from wastewater. | campylobacter jejuni is one of the leading foodborne pathogens worldwide. c. jejuni is isolated from a wide range of foods, domestic animals, wildlife, and environmental sources. the currently available culture-based isolation methods are not highly effective for wastewater samples due to the low number of c. jejuni in the midst of competing bacteria. to detect and isolate c. jejuni from wastewater samples, in this study, we evaluated a few different enrichment conditions using five different an ... | 2016 | 27617011 |
| molecular epidemiology of campylobacter jejuni infection in israel-a nationwide study. | the incidence of campylobacter infection in israel, particularly among children <2 years of age, has risen over the last decade and became one of the highest among industrialized countries. this study explored the molecular epidemiology of campylobacter jejuni in israel over a decade (2003-2012) using multilocus sequence typing (mlst) combined with demographic metadata. representative clinical isolates (438) from a large national repository together with selected veterinary isolates (74) were su ... | 2016 | 27615719 |
| strain dependent genetic networks for antibiotic-sensitivity in a bacterial pathogen with a large pan-genome. | the interaction between an antibiotic and bacterium is not merely restricted to the drug and its direct target, rather antibiotic induced stress seems to resonate through the bacterium, creating selective pressures that drive the emergence of adaptive mutations not only in the direct target, but in genes involved in many different fundamental processes as well. surprisingly, it has been shown that adaptive mutations do not necessarily have the same effect in all species, indicating that the gene ... | 2016 | 27607357 |
| characterizing the syphilis-causing treponema pallidum ssp. pallidum proteome using complementary mass spectrometry. | the spirochete bacterium treponema pallidum ssp. pallidum is the etiological agent of syphilis, a chronic multistage disease. little is known about the global t. pallidum proteome, therefore mass spectrometry studies are needed to bring insights into pathogenicity and protein expression profiles during infection. | 2016 | 27606673 |
| campylobacter jejuni: a rare agent in a child with peritoneal dialysis-related peritonitis. | peritonitis is a serious problem in children receiving peritoneal dialysis. campylobacter jejuni is an unusual cause of peritonitis. a 10-year-old boy who had end stage renal failure due to atypical hemolytic uremic syndrome was admitted to our hospital with abdominal pain and fever. peritoneal dialysis fluid was cloudy and microscopic examination showed abundant leukocytes. intraperitoneal cefepime treatment was started. campylobacter jejuni was isolated from peritoneal dialysis fluid culture a ... | 2016 | 27606662 |
| microbial diagnostic microarrays for the detection and typing of food- and water-borne (bacterial) pathogens. | reliable and sensitive pathogen detection in clinical and environmental (including food and water) samples is of greatest importance for public health. standard microbiological methods have several limitations and improved alternatives are needed. most important requirements for reliable analysis include: (i) specificity; (ii) sensitivity; (iii) multiplexing potential; (iv) robustness; (v) speed; (vi) automation potential; and (vii) low cost. microarray technology can, through its very nature, f ... | 2011 | 27605332 |
| microbial diagnostic microarrays for the detection and typing of food- and water-borne (bacterial) pathogens. | reliable and sensitive pathogen detection in clinical and environmental (including food and water) samples is of greatest importance for public health. standard microbiological methods have several limitations and improved alternatives are needed. most important requirements for reliable analysis include: (i) specificity; (ii) sensitivity; (iii) multiplexing potential; (iv) robustness; (v) speed; (vi) automation potential; and (vii) low cost. microarray technology can, through its very nature, f ... | 2011 | 27605332 |
| evidence of allosteric enzyme regulation via changes in conformational dynamics: a hydrogen/deuterium exchange investigation of dihydrodipicolinate synthase. | dihydrodipicolinate synthase is a tetrameric enzyme of the diaminopimelate pathway in bacteria and plants. the protein catalyzes the condensation of pyruvate (pyr) and aspartate semialdehyde en route to the end product lysine (lys). dihydrodipicolinate synthase from campylobacter jejuni (cjdhdps) is allosterically inhibited by lys. cjdhdps is a promising antibiotic target, as highlighted by the recent development of a potent bis-lysine (bislys) inhibitor. the mechanism whereby lys and bislys all ... | 2016 | 27604304 |
| acute gastroenteritis and campylobacteriosis in swiss primary care: the viewpoint of general practitioners. | acute gastroenteritis (ag) is frequently caused by infectious intestinal diseases (iid) including food- and waterborne pathogens of public health importance. among these pathogens, campylobacter spp. plays a major role. many european countries monitor selected iids within disease surveillance systems. in switzerland, the information on iids is restricted to limited surveillance data, while no data is available for ag. we conducted a qualitative study among swiss general practitioners (gps) to in ... | 2016 | 27603141 |
| sensitivity to enterocins of thermophilic campylobacter spp. from different poultry species. | campylobacter spp. have been isolated from various animals, including poultry. they are rapidly transmitted throughout broiler sheds by the fecal-oral route. a promising strategy to reduce campylobacter spp. in poultry may be done due to the beneficial properties of probiotic bacteria and their bacteriocins. in this study, inhibition spectrum/activity of different enterocins was evaluated against campylobacter spp. (isolated from different poultry) to indicate further practical use of enterocins ... | 2016 | 27602434 |
| point mutations in the major outer membrane protein drive hypervirulence of a rapidly expanding clone of campylobacter jejuni. | infections due to clonal expansion of highly virulent bacterial strains are clear and present threats to human and animal health. association of genetic changes with disease is now a routine, but identification of causative mutations that enable disease remains difficult. campylobacter jejuni is an important zoonotic pathogen transmitted to humans mainly via the foodborne route. c. jejuni typically colonizes the gut, but a hypervirulent and rapidly expanding clone of c. jejuni recently emerged, ... | 2016 | 27601641 |
| isolation of clostridium limosum from an outbreak of metritis in farmed mink. | an outbreak of sudden death of pregnant farmed mink in finland occurred during the busiest whelping period in the spring of 2013. the affected farms were all located in western finland in a rather narrow geographic area, ostrobothnia. dead mink from 22 farms were submitted for laboratory diagnostics to the finnish food safety authority (evira). the carcasses were necropsied and tissue specimens were prepared for histology. samples of internal organs and peritoneal fluid were cultured bacteriolog ... | 2016 | 27600916 |
| isolation of clostridium limosum from an outbreak of metritis in farmed mink. | an outbreak of sudden death of pregnant farmed mink in finland occurred during the busiest whelping period in the spring of 2013. the affected farms were all located in western finland in a rather narrow geographic area, ostrobothnia. dead mink from 22 farms were submitted for laboratory diagnostics to the finnish food safety authority (evira). the carcasses were necropsied and tissue specimens were prepared for histology. samples of internal organs and peritoneal fluid were cultured bacteriolog ... | 2016 | 27600916 |
| assessing bacterial interactions using carbohydrate-based microarrays. | carbohydrates play a crucial role in host-microorganism interactions and many host glycoconjugates are receptors or co-receptors for microbial binding. host glycosylation varies with species and location in the body, and this contributes to species specificity and tropism of commensal and pathogenic bacteria. additionally, bacterial glycosylation is often the first bacterial molecular species encountered and responded to by the host system. accordingly, characterising and identifying the exact s ... | 2015 | 27600247 |
| a liposome-based approach to the integrated multi-component antigen microarrays. | this report describes an experimental procedure for constructing integrated lipid, carbohydrate, and protein microarrays. in essence, it prints liposomes on nitrocellulose-coated micro-glass slides, a biochip substrate for spotting protein and carbohydrate microarrays, and the substances that can form liposomes (homo-liposomes) or can be incorporated into liposomes (hetero-liposomes) are suitable for microarray construction using existing microarray spotting devices. importantly, this technology ... | 2015 | 27600243 |
| infectious diseases: pathophysiology, diagnostics and prevention. | infectious diseases occur very frequently in children and adults. novel diagnostic methods have permitted us to expand our knowledge on their epidemiology and pathophysiology [1].[...]. | 2016 | 27598145 |
| an unusual case of recurrent guillain-barré syndrome with normal cerebrospinal fluid protein levels: a case report. | guillain-barré syndrome is an acquired polyradiculo-neuropathy, often preceded by an antecedent event. it is a monophasic disease but a recurrence rate of 1-6 % is documented in a subset group of patients. patients with guillain-barré syndrome show cerebrospinal fluid albuminocytologic dissociation. normal cerebrospinal fluid protein levels during both initial and recurrent episodes of guillain-barré syndrome is a rare occurrence and has not been described earlier in the literature. | 2016 | 27596231 |
| macrolide resistance mechanisms and virulence factors in erythromycin-resistant campylobacter species isolated from chicken and swine feces and carcasses. | resistance to antimicrobials was measured in 73 isolates of campylobacter jejuni (c. jejuni) and 121 isolates of campylobacter coli (c. coli) from chicken and swine feces and carcasses in korea. both bacterial species showed the highest resistance to (fluoro) quinolones (ciprofloxacin and nalidixic acid) out of the nine antimicrobials tested. erythromycin resistance was much higher in c. coli (19.0%, 23/121) than in c. jejuni (6.8%, 5/73). the mutation in the 23s rrna gene was primarily responsi ... | 2017 | 27593510 |
| role of human milk oligosaccharides in group b streptococcus colonisation. | group b streptococcus (gbs) infection is a major cause of morbidity and mortality in infants. the major risk factor for gbs disease is maternal and subsequent infant colonisation. it is unknown whether human milk oligosaccharides (hmos) protect against gbs colonisation. hmo production is genetically determined and linked to the lewis antigen system. we aimed to investigate the association between hmos and infant gbs colonisation between birth and postnatal day 90. rectovaginal swabs were collect ... | 2016 | 27588204 |
| clustering analysis of proteins from microbial genomes at multiple levels of resolution. | microbial genomes at the national center for biotechnology information (ncbi) represent a large collection of more than 35,000 assemblies. there are several complexities associated with the data: a great variation in sampling density since human pathogens are densely sampled while other bacteria are less represented; different protein families occur in annotations with different frequencies; and the quality of genome annotation varies greatly. in order to extract useful information from these so ... | 2016 | 27586436 |
| decay rates of zoonotic pathogens and viral surrogates in soils amended with biosolids and manures and comparison of qpcr and culture derived rates. | the purpose of this study was to establish inactivation decay constants of foodborne pathogens and coliphage in clay and sandy soils for future "downstream" analyses such as quantitative microbial risk analysis and to compare cultivation-dependent and -independent (e.g. qpcr) methods. | 2016 | 27585434 |
| development of multiplex-mismatch amplification mutation-pcr assay for simultaneous detection of campylobacter jejuni and mutation in gyra gene related to fluoroquinolone resistance. | campylobacter jejuni, a foodborne pathogen, is the major cause of enteritis in humans worldwide, however, its increasing resistance to fluoroquinolones reported recently is of a major concern. in the present study, multiplex-mismatch amplification mutation assay-polymerase chain reaction (mmama-pcr) was developed for the first time with the aim to quickly identify c. jejuni and to detect the single nucleotide mutation (c-257 to t) frequently observed in gyra gene, associated with the acquisition ... | 2016 | 27584716 |
| weakness in pregnancy - expect the unexpected. | guillain-barré syndrome (gbs) is rare in pregnancy with an incidence estimated to be between 1.2 and 1.9 cases per 100,000 people annually, and it is generally accepted that it carries a high maternal risk. delayed diagnosis is common because the initial non-specific symptoms may mimic changes in pregnancy. gbs should be considered in any pregnant patient complaining of muscle weakness, general malaise, tingling of the fingers and respiratory discomfort. this case aims to highlight the importanc ... | 2008 | 27582795 |
| how rheumatoid arthritis can result from provocation of the immune system by microorganisms and viruses. | the pathogenesis of rheumatoid arthritis (ra), similar to development of a majority of inflammatory and autoimmune disorders, is largely due to an inappropriate or inadequate immune response to environmental challenges. among these challenges, infectious agents are the undisputed leaders. since the 1870s, an impressive list of microorganisms suspected of provoking ra has formed, and the list is still growing. although a definite causative link between a specific infectious agent and the disease ... | 2016 | 27582741 |
| high-throughput recombinant protein expression in escherichia coli: current status and future perspectives. | the ease of genetic manipulation, low cost, rapid growth and number of previous studies have made escherichia coli one of the most widely used microorganism species for producing recombinant proteins. in this post-genomic era, challenges remain to rapidly express and purify large numbers of proteins for academic and commercial purposes in a high-throughput manner. in this review, we describe several state-of-the-art approaches that are suitable for the cloning, expression and purification, condu ... | 2016 | 27581654 |
| prevalence and characteristics of salmonella and campylobacter in retail poultry meat in japan. | this study was undertaken to determine the prevalence, antimicrobial susceptibility, and genetic relatedness of salmonella enterica subsp. enterica and campylobacter spp. in poultry meat, and to analyze the association of genetic types of these bacteria with their geographical distribution and antimicrobial resistance profiles. fifty-four and 71 of 100 samples were found to be contaminated with salmonella and campylobacter, respectively. nine salmonella serotypes were found, including s. enteric ... | 2016 | 27580577 |
| triclosan resistome from metagenome reveals diverse enoyl acyl carrier protein reductases and selective enrichment of triclosan resistance genes. | triclosan (tcs) is a widely used antimicrobial agent and tcs resistance is considered to have evolved in diverse organisms with extensive use of tcs, but distribution of tcs resistance has not been well characterized. functional screening of the soil metagenome in this study has revealed that a variety of target enoyl acyl carrier protein reductases (enr) homologues are responsible for the majority of tcs resistance. diverse enrs similar to 7-α-hydroxysteroid dehydrogenase (7-α-hsdh), fabg, or t ... | 2016 | 27577999 |
| mapping autoantigen epitopes: molecular insights into autoantibody-associated disorders of the nervous system. | our knowledge of autoantibody-associated diseases of the central (cns) and peripheral (pns) nervous systems has expanded greatly over the recent years. a number of extracellular and intracellular autoantigens have been identified, and there is no doubt that this field will continue to expand as more autoantigens are discovered as a result of improved clinical awareness and methodological practice. in recent years, interest has shifted to uncover the target epitopes of these autoantibodies. | 2016 | 27577085 |
| outbreak of campylobacteriosis associated with raccoon contact at a wildlife rehabilitation centre, minnesota, 2013. | campylobacteriosis is an enteric illness caused by bacteria of the genus campylobacter. there are approximately 900 culture-confirmed cases of campylobacteriosis reported annually to the minnesota department of health (mdh). case patients are interviewed about risk factors, including foods eaten, recreational and drinking water exposures and animal contact. in september 2013, mdh identified two campylobacter jejuni cases who reported working at the same wildlife rehabilitation centre before illn ... | 2017 | 27576067 |
| invasive behavior of campylobacter jejuni in immunosuppressed chicken. | campylobacter jejuni is a predominant cause of gastroenteritis in humans but rather harmless in chickens. the basis of this difference is unknown. we investigated the effect of the chicken immune defense on the behavior of c. jejuni using glucocorticoid (gc)-treated and mock-treated 17-day old ross 308 chicken bearing in mind that gcs have immunosuppressive effects and dampen the innate immune response. the effect of gc administration on the behavior of c. jejuni was compared with that on infect ... | 2016 | 27574876 |
| bacterial n-glycosylation efficiency is dependent on the structural context of target sequons. | site selectivity of protein n-linked glycosylation is dependent on many factors, including accessibility of the modification site, amino acid composition of the glycosylation consensus sequence, and cellular localization of target proteins. previous studies have shown that the bacterial oligosaccharyltransferase, pglb, of campylobacter jejuni favors acceptor proteins with consensus sequences ((d/e)x1nx2(s/t), where x1,2 ≠ proline) in flexible, solvent-exposed motifs; however, several native glyc ... | 2016 | 27573243 |
| an overview of the control of bacterial pathogens in cattle manure. | cattle manure harbors microbial constituents that make it a potential source of pollution in the environment and infections in humans. knowledge of, and microbial assessment of, manure is crucial in a bid to prevent public health and environmental hazards through the development of better management practices and policies that should govern manure handling. physical, chemical and biological methods to reduce pathogen population in manure do exist, but are faced with challenges such as cost, odor ... | 2016 | 27571092 |
| antimicrobial resistance of campylobacter isolates from small scale and backyard chicken in kenya. | thermophilic campylobacter species are a major cause of bacterial foodborne diarrhoea in humans worldwide. poultry and their products are the predominant source for human campylobacteriosis. resistance of campylobacter to antibiotics is increasing worldwide, but little is known about the antibiotic resistance in campylobacter isolated from chicken in kenya. in this study, 35 suspected campylobacter strains isolated from faeces and cloacal swabs of chicken were tested for their susceptibility to ... | 2016 | 27570543 |
| campylobacters and their bacteriophages from chicken liver: the prospect for phage biocontrol. | consumption of foods containing chicken liver has been associated with campylobacter enteritis. campylobacters can contaminate the surface of livers post-mortem but can also arise through systemic infection of colonising bacteria in live birds. the use of bacteriophage to reduce levels of campylobacter entering the food chain is a promising intervention approach but most phages have been isolated from chicken excreta. this study examined the incidence and contamination levels of campylobacter an ... | 2016 | 27565524 |
| program and abstracts from the canadian digestive diseases week™ 2016. | 2016 | 27563641 | |
| cinnamaldehyde, carvacrol and organic acids affect gene expression of selected oxidative stress and inflammation markers in ipec-j2 cells exposed to salmonella typhimurium. | essential oils and organic acids are used as feed additives to improve health status and reduce colonization with pathogens. although bactericidal in vitro, concentrations achieved in the animal gut are probably not lethal to pathogens. the aim of this study was to investigate the effects of cinnamaldehyde, carvacrol and cinnamic, lactic and propionic acids on the ability of salmonella typhimurium atcc 14028 (st) to invade intestinal epithelial cells (ipec-j2) and on the expression levels of imm ... | 2016 | 27561686 |
| a journey of cytolethal distending toxins through cell membranes. | the multifunctional role of lipids as structural components of membranes, signaling molecules, and metabolic substrates makes them an ideal partner for pathogens to hijack host cell processes for their own survival. the properties and composition of unique membrane micro-domains such as membrane rafts make these regions a natural target for pathogens as it affords them an opportunity to hijack cell signaling and intracellular trafficking pathways. cytolethal distending toxins (cdts), members of ... | 2016 | 27559534 |
| comparative methylome analysis of the occasional ruminant respiratory pathogen bibersteinia trehalosi. | we examined and compared both the methylomes and the modification-related gene content of four sequenced strains of bibersteinia trehalosi isolated from the nasopharyngeal tracts of nebraska cattle with symptoms of bovine respiratory disease complex. the methylation patterns and the encoded dna methyltransferase (mtase) gene sets were different between each strain, with the only common pattern being that of dam (gatc). among the observed patterns were three novel motifs attributable to type i re ... | 2016 | 27556252 |
| prophylactic bacteriophage administration more effective than post-infection administration in reducing salmonella enterica serovar enteritidis shedding in quail. | infections caused by salmonella bacteria, often through poultry products, are a serious public health issue. because of drawbacks associated with antibiotic prophylaxis, alternative treatments are sought. bacterial viruses (bacteriophages) may provide an effective alternative, but concerns remain with respect to bacteriophage stability and effectiveness. to this end, we assessed the stability of a novel bacteriophage isolated from poultry excreta, siphovirus pse, and its effectiveness in reducin ... | 2016 | 27555842 |
| viral proteins expressed in the protozoan parasite eimeria tenella are detected by the chicken immune system. | eimeria species are parasitic protozoa that cause coccidiosis, an intestinal disease commonly characterised by malabsorption, diarrhoea and haemorrhage that is particularly important in chickens. vaccination against chicken coccidiosis is effective using wild-type or attenuated live parasite lines. the development of protocols to express foreign proteins in eimeria species has opened up the possibility of using eimeria live vaccines to deliver heterologous antigens and function as multivalent va ... | 2016 | 27553200 |
| multiplex pcr assay for identifi cation and differentiation of campylobacter jejuni and campylobacter coli isolates. | campylobacter spp. are important causative agents of gastrointestinal infections in humans. the most frequently isolated strains of this bacterial genus are campylobacter jejuni and campylobacter coli. to date, genetic methods for bacterial identification have not been used in bulgaria. we optimized the multiplex psr assay to identify campylobacter spp. and differentiate c. jejuni from c. coli in clinical isolates. we also compared this method with the routinely used biochemical methods. | 2017 | 27552785 |
| enteropathogens in children less than 5 years of age with acute diarrhea: a 5-year surveillance study in the southeast coast of china. | diarrhea is the second most common cause of death among children less than 5 years of age worldwide. the etiological agents of diarrhea in the southeast coastal area of china were studied from july 2009 to december 2014. | 2016 | 27544130 |
| whole-genome sequences of agricultural, host-associated campylobacter coli and campylobacter jejuni strains. | we report here the genome sequences of four agricultural, multidrug-resistant campylobacter spp.: c. coli 11601 and c. jejuni 11601md, isolated from turkey cecum and jejunum, respectively, and c. coli 6067 and c. coli 6461, isolated from turkey-house water and swine feces, respectively. the genomes provide insights on campylobacter antimicrobial resistance and host adaptations. | 2016 | 27540063 |
| total biosynthesis of legionaminic acid, a bacterial sialic acid analogue. | legionaminic acid, leg5,7ac2 , a nonulosonic acid like 5-acetamido neuraminic acid (neu5ac, sialic acid), is found in cell surface glycoconjugates of bacteria including the pathogens campylobacter jejuni, acinetobacter baumanii and legionella pneumophila. the presence of leg5,7ac2 has been correlated with virulence in humans by mechanisms that likely involve subversion of the host's immune system or interactions with host cell surfaces due to its similarity to neu5ac. investigation into its role ... | 2016 | 27538580 |
| the o-antigen flippase wzk can substitute for murj in peptidoglycan synthesis in helicobacter pylori and escherichia coli. | the peptidoglycan (pg) cell wall is an essential component of the cell envelope of most bacteria. biogenesis of pg involves a lipid-linked disaccharide-pentapeptide intermediate called lipid ii, which must be translocated across the cytoplasmic membrane after it is synthesized in the inner leaflet of this bilayer. accordingly, it has been demonstrated that murj, the proposed lipid ii flippase in escherichia coli, is required for pg biogenesis, and thereby viability. in contrast, murj is not esse ... | 2016 | 27537185 |
| veterinary medicine needs new green antimicrobial drugs. | given that: (1) the worldwide consumption of antimicrobial drugs (amds) used in food-producing animals will increase over the coming decades; (2) the prudent use of amds will not suffice to stem the rise in human antimicrobial resistance (amr) of animal origin; (3) alternatives to amd use are not available or not implementable, there is an urgent need to develop novel amds for food-producing animals. this is not for animal health reasons, but to break the link between human and animal resistomes ... | 2016 | 27536285 |
| [invasive campylobacter jejuni/coli infections: 9 case reports at a single center between 2000 and 2015, and a review of literature describing japanese patients]. | there have been few coherent reports on extraintestinal infection or bacteremia caused by campylobacter jejuni (c. jejuni) or c. coli in japan. to clarify the clinical and microbiological characteristics of invasive infections caused by these two species, we retrospectively analyzed the records of patients from whom these pathogens had been isolated from sterile sites between 2000 and 2015. during this study period, we identified 9 patients. the clinical syndrome of all of these patients was bac ... | 2016 | 27529964 |
| mechanisms of outer membrane vesicle entry into host cells. | bacterial outer membrane vesicles (omvs) are nano-sized compartments consisting of a lipid bilayer that encapsulates periplasm-derived, luminal content. omvs, which pinch off of gram-negative bacteria, are now recognized as a generalized secretion pathway which provides a means to transfer cargo to other bacterial cells as well as eukaryotic cells. compared with other secretion systems, omvs can transfer a chemically extremely diverse range of cargo, including small molecules, nucleic acids, pro ... | 2016 | 27529760 |