Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| the application of genome editing in studying hearing loss. | targeted genome editing mediated by clustered, regularly interspaced, short palindromic repeat (crispr)/crispr-associated nuclease 9 (cas9) technology has emerged as one of the most powerful tools to study gene functions, and with potential to treat genetic disorders. hearing loss is one of the most common sensory disorders, affecting approximately 1 in 500 newborns with no treatment. mutations of inner ear genes contribute to the largest portion of genetic deafness. the simplicity and robustnes ... | 2015 | 25987504 |
| microbiome profiles in periodontitis in relation to host and disease characteristics. | periodontitis is an inflammatory condition that affects the supporting tissues surrounding teeth. the occurrence of periodontitis is associated with shifts in the structure of the communities that inhabit the gingival sulcus. although great inter-subject variability in the subgingival microbiome has been observed in subjects with periodontitis, it is unclear whether distinct community types exist and if differences in microbial signatures correlate with host characteristics or with the variable ... | 2015 | 25984952 |
| porphyromonas gingivalis-derived rgpa-kgp complex activates the macrophage urokinase plasminogen activator system: implications for periodontitis. | urokinase plasminogen activator (upa) converts plasminogen to plasmin, resulting in a proteolytic cascade that has been implicated in tissue destruction during inflammation. periodontitis is a highly prevalent chronic inflammatory disease characterized by destruction of the tissue and bone that support the teeth. we demonstrate that stimulation of macrophages with the arginine- and lysine-specific cysteine protease complex (rgpa-kgp complex), produced by the keystone pathogen porphyromonas gingi ... | 2015 | 25979345 |
| association of treponeme species with atypical foot lesions in goats. | five uk goat farms with high levels of lameness (prevalence 14-67 per cent) were investigated. on two farms (farms 1 and 2), the animals presented with typical footrot lesions. the remaining three farms (farms 3, 4 and 5) presented with infected lesions on the foot that did not resemble footrot. these lesions were observed to start from the white line or sole but the interdigital space was rarely affected. swabs were processed by pcr to assess the presence of dichelobacter nodosus and three spec ... | 2015 | 25977404 |
| periodontitis associated with plasminogen deficiency: a case report. | plasminogen deficiency is a rare autosomal recessive disease, which is associated with aggressive periodontitis and gingival enlargement. previously described treatments of plasminogen deficiency associated periodontitis have shown limited success. this is the first case report indicating a successful therapy approach consisting of a non-surgical supra- and subgingival debridement in combination with an adjunctive systemic antibiotic therapy and a strict supportive periodontal regimen over an ob ... | 2015 | 25971786 |
| increased infection with key periodontal pathogens during gestational diabetes mellitus. | gestational diabetes mellitus (gdm), gingivitis, infection with specific periodontal pathogens and systemic inflammation each increase the risk for poor pregnancy outcome. we set out to monitor the interactions of gingivitis and gdm with respect to oral infection and the systemic inflammatory burden. | 2015 | 25959628 |
| the nlrp3 inflammasome is a pathogen sensor for invasive entamoeba histolytica via activation of α5β1 integrin at the macrophage-amebae intercellular junction. | entamoeba histolytica (eh) is an extracellular protozoan parasite of humans that invades the colon to cause life-threatening intestinal and extra-intestinal amebiasis. colonized eh is asymptomatic, however, when trophozoites adhere to host cells there is a considerable inflammatory response that is critical in the pathogenesis of amebiasis. the host and/or parasite factors that trigger the inflammatory response to invading eh are not well understood. we recently identified that eh adherence to m ... | 2015 | 25955828 |
| klikk proteases of tannerella forsythia: putative virulence factors with a unique domain structure. | comparative genomics of virulent tannerella forsythia atcc 43037 and a close health-associated relative, tannerella bu063, revealed, in the latter, the absence of an entire array of genes encoding putative secretory proteases that possess a nearly identical c-terminal domain (ctd) that ends with a -lys-leu-ile-lys-lys motif. this observation suggests that these proteins, referred to as klikk proteases, may function as virulence factors. re-sequencing of the loci of the klikk proteases found only ... | 2015 | 25954253 |
| outer membrane vesicles of tannerella forsythia: biogenesis, composition, and virulence. | tannerella forsythia is the only 'red-complex' bacterium covered by an s-layer, which has been shown to affect virulence. here, outer membrane vesicles (omvs) enriched with putative glycoproteins are described as a new addition to the virulence repertoire of t. forsythia. investigations of this bacterium are hampered by its fastidious growth requirements and the recently discovered mismatch of the available genome sequence (92a2 = atcc baa-2717) and the widely used t. forsythia strain (atcc 4303 ... | 2015 | 25953484 |
| microbial flora in chronic periodontitis: study at a tertiary health care center from north karnataka. | periodontitis is a major public health problem in india with a prevalence of 60-80%. if untreated it acts as a risk factor for systemic diseases. data on anaerobic periodontal microflora in the indian population is very scarce. hence, this study was undertaken to know the nature of oral microbiota in chronic periodontitis in this region of india and also the semiquantitative study in pre- and post-treatment group and to determine antibiotic susceptibility pattern for aerobic isolates. | 2015 | 25949060 |
| liver x receptors contribute to periodontal pathogen-elicited inflammation and oral bone loss. | periodontal diseases are chronic oral inflammatory diseases that are polymicrobial in nature. the presence of specific bacteria in subgingival plaque such as porphyromonas gingivalis is associated with microbial dysbiosis and the modulation of host immune response. bacterially elicited innate immune activation and inflammation are key elements implicated in the destruction of soft and hard tissues supporting the teeth. liver x receptors (lxrs) are nuclear hormone receptors with important functio ... | 2015 | 25946408 |
| evidence for posttranslational protein flavinylation in the syphilis spirochete treponema pallidum: structural and biochemical insights from the catalytic core of a periplasmic flavin-trafficking protein. | the syphilis spirochete treponema pallidum is an important human pathogen but a highly enigmatic bacterium that cannot be cultivated in vitro. t. pallidum lacks many biosynthetic pathways and therefore has evolved the capability to exploit host-derived metabolites via its periplasmic lipoprotein repertoire. we recently reported a flavin-trafficking protein in t. pallidum (ftp_tp; tp0796) as the first bacterial metal-dependent flavin adenine dinucleotide (fad) pyrophosphatase that hydrolyzes fad ... | 2015 | 25944861 |
| halitosis in otorhinolaryngology practice. | halitosis is a common and devastating condition, which may affect up to 1/3 of the population. it can be classified either as genuine halitosis, pseudohalitosis, or halitophobia. genuine halitosis is more common and usually related to an organic pathology such as periodontitis. malodour molecules such as sulfur compounds that arise from bacterial interactions generate the basis of oral malodour. pathologies of the tongue, poor oral hygiene, deep caries, cryptic tonsillary hypertrophia, and postn ... | 2015 | 25938086 |
| development of in vitro denture biofilm models for halitosis related bacteria and their application in testing the efficacy of antimicrobial agents. | since dentures can serve as a reservoir for halitosis-causing oral bacteria, halitosis development is a concern for denture wearers. in this study, we surveyed the prevalence of four selected halitosis-related species (fusobacterium nucleatum, tannerella forsythia, veillonella atypica and klebsiella pneumoniae) in clinical denture plaque samples, and developed denture biofilm models for these species in vitro to facilitate assessment of antimicrobial treatment efficacy. design : denture plaque f ... | 2015 | 25926895 |
| defining functional signatures of dysbiosis in periodontitis progression. | periodontitis is a common inflammatory disease that leads to tooth loss and has been linked to cardiovascular disease and diabetes mellitus. the periodontal microbiome is highly diverse, and metatranscriptomic studies have indicated that the genes that are expressed by the microbiota are more relevant than the microbial composition in the pathogenesis of periodontitis. a recent study of early metabolic activities in the dysbiotic microbiome reveals a functional signature that distinguishes perio ... | 2015 | 25926890 |
| periodontal status and pathogenic bacteria after gastric bypass: a cohort study. | the aim this study was to evaluate the influence of gastric bypass surgery (gbs) on periodontal disease and quantify the periodontopathogenic bacteria in patients undergoing this surgery. | 2015 | 25924695 |
| identification of subgingival periodontal pathogens and association with the severity of periodontitis in patients with chronic kidney diseases: a cross-sectional study. | the aim of our study was to assess the subgingival profile of 9 periodontal pathogens, by means of real-time pcr, in a group of predialysis chronic kidney disease patients with and without periodontal disease and to identify the risk factors associated with periodontal disease in these patients. | 2015 | 25922833 |
| functional signatures of oral dysbiosis during periodontitis progression revealed by microbial metatranscriptome analysis. | periodontitis is a polymicrobial biofilm-induced inflammatory disease that affects 743 million people worldwide. the current model to explain periodontitis progression proposes that changes in the relative abundance of members of the oral microbiome lead to dysbiosis in the host-microbiome crosstalk and then to inflammation and bone loss. using combined metagenome/metatranscriptome analysis of the subgingival microbiome in progressing and non-progressing sites, we have characterized the distinct ... | 2015 | 25918553 |
| interaction of spirochetes with the host fibrinolytic system and potential roles in pathogenesis. | the pathogenic spirochetes borrelia burgdorferi, b. hermsii, b. recurrentis, treponema denticola and leptospira spp. are the etiologic agents of lyme disease, relapsing fever, periodontitis and leptospirosis, respectively. lyme borreliosis is a multi-systemic disorder and the most prevalent tick-borne disease in the northern hemisphere. tick-borne relapsing fever is persistent in endemic areas worldwide, representing a significant burden in some african regions. periodontal disease, a chronic in ... | 2016 | 25914944 |
| selenium utilization in thioredoxin and catalytic advantage provided by selenocysteine. | thioredoxin (trx) is a major thiol-disulfide reductase that plays a role in many biological processes, including dna replication and redox signaling. although selenocysteine (sec)-containing trxs have been identified in certain bacteria, their enzymatic properties have not been characterized. in this study, we expressed a selenoprotein trx from treponema denticola, an oral spirochete, in escherichia coli and characterized this selenoenzyme and its natural cysteine (cys) homologue using e. coli t ... | 2015 | 25912135 |
| efficient genome editing in clostridium cellulolyticum via crispr-cas9 nickase. | the crispr-cas9 system is a powerful and revolutionary genome-editing tool for eukaryotic genomes, but its use in bacterial genomes is very limited. here, we investigated the use of the streptococcus pyogenes crispr-cas9 system in editing the genome of clostridium cellulolyticum, a model microorganism for bioenergy research. wild-type cas9-induced double-strand breaks were lethal to c. cellulolyticum due to the minimal expression of nonhomologous end joining (nhej) components in this strain. to ... | 2015 | 25911483 |
| intracellular concentrations of borrelia burgdorferi cyclic di-amp are not changed by altered expression of the cdaa synthase. | the second messenger nucleotide cyclic diadenylate monophosphate (c-di-amp) has been identified in several species of gram positive bacteria and chlamydia trachomatis. this molecule has been associated with bacterial cell division, cell wall biosynthesis and phosphate metabolism, and with induction of type i interferon responses by host cells. we demonstrate that b. burgdorferi produces a c-di-amp synthase, which we designated cdaa. both cdaa and c-di-amp levels are very low in cultured b. burgd ... | 2015 | 25906393 |
| functional advantages of porphyromonas gingivalis vesicles. | porphyromonas gingivalis is a keystone pathogen of periodontitis. outer membrane vesicles (omvs) have been considered as both offense and defense components of this bacterium. previous studies indicated that like their originating cells, p. gingivalis vesicles, are able to invade oral epithelial cells and gingival fibroblasts, in order to promote aggregation of some specific oral bacteria and to induce host immune responses. in the present study, we investigated the invasive efficiency of p. gin ... | 2015 | 25897780 |
| comprehensive functional analysis of the 18 vibrio cholerae n16961 toxin-antitoxin systems substantiates their role in stabilizing the superintegron. | the role of chromosomal toxin-antitoxin (ta) systems, which are ubiquitous within the genomes of free-living bacteria, is still debated. we have scanned the vibrio cholerae n16961 genome for class 2 ta genes and identified 18 gene pair candidates. interestingly, all but one are located in the chromosome 2 superintegron (si). the single ta found outside the si is located on chromosome 1 and is related to the well-characterized hipab family, which is known to play a role in antibiotic persistence. ... | 2015 | 25897030 |
| bacterial profile of aggressive periodontitis in morocco: a cross-sectional study. | aggressive periodontitis (agp) is one of the most severe forms of periodontal diseases. in morocco, aggregatibacter actinomycetemcomitans has been strongly associated with agp, however limited knowledge is available about the implication of other periodontal pathogens in this entity. therefore, the main aim of this study was to evaluate the composition of the subgingival microbiota in moroccan patients with agp. | 2015 | 25888404 |
| clinical, microbiological, and immunological aspects of healthy versus peri-implantitis tissue in full arch reconstruction patients: a prospective cross-sectional study. | due to the world-wide increase in treatments involving implant placement, the incidence of peri-implant disease is increasing. late implant failure is the result of the inability to maintain osseointegration, whose most important cause is peri-implantitis. the aim of this study was to analyze the clinical, microbiological, and immunological aspects in the peri-implant sulcus fluid (pisf) of patients with healthy dental implants and patients with peri-implantitis. | 2015 | 25888355 |
| kanamycin resistance cassette for genetic manipulation of treponema denticola. | treponema denticola has been recognized as an important oral pathogen of the "red complex" bacterial consortium that is associated with the pathogenesis of endodontal and periodontal diseases. however, little is known about the virulence of t. denticola due to its recalcitrant genetic system. the difficulty in genetically manipulating oral spirochetes is partially due to the lack of antibiotic resistance cassettes that are useful for gene complementation following allelic replacement mutagenesis ... | 2015 | 25888173 |
| development and pyrosequencing analysis of an in-vitro oral biofilm model. | dental caries and periodontal disease are the commonest bacterial diseases of man and can result in tooth loss. the principal method of prevention is the mechanical removal of dental plaque augmented by active agents incorporated into toothpastes and mouthrinses. in-vitro assays that include complex oral bacterial biofilms are required to accurately predict the efficacy of novel active agents in vivo. the aim of this study was to develop an oral biofilm model using the calgary biofilm device (cb ... | 2015 | 25880819 |
| odanacatib, a cathepsin k-specific inhibitor, inhibits inflammation and bone loss caused by periodontal diseases. | periodontitis is a bacteria-induced inflammatory disease mainly affecting periodontal tissues, leading to periodontal inflammation, bone breakdown, and loss of the tooth. the main obstacle for treating periodontitis effectively is the difficulty in finding a target that can inhibit bone loss and inflammation simultaneously. recent studies showed that cathepsin k (ctsk) might have functions in the immune system besides its role in osteoclasts. thus, targeting ctsk would have a potential therapeut ... | 2015 | 25879791 |
| exploring the association between morgellons disease and lyme disease: identification of borrelia burgdorferi in morgellons disease patients. | morgellons disease (md) is a complex skin disorder characterized by ulcerating lesions that have protruding or embedded filaments. many clinicians refer to this condition as delusional parasitosis or delusional infestation and consider the filaments to be introduced textile fibers. in contrast, recent studies indicate that md is a true somatic illness associated with tickborne infection, that the filaments are keratin and collagen in composition and that they result from proliferation and activa ... | 2015 | 25879673 |
| laser supported reduction of specific microorganisms in the periodontal pocket with the aid of an er,cr:ysgg laser: a pilot study. | the aim of this study was to evaluate the effectiveness of a radial firing tip of an er,cr:ysgg laser as an adjunct to a nonsurgical periodontal treatment. | 2015 | 25879057 |
| on putative periodontal pathogens: an epidemiological perspective. | the current understanding on the role of microbiology on periodontitis causation is reviewed. an appraisal of the literature reveals several issues that have limited the attempts to investigate candidate periodontal pathogens as causes of periodontitis and confirms that only limited epidemiological evidence is available. several aspects of the contemporary understanding on causal inference are discussed with examples for periodontitis. | 2015 | 25874553 |
| relationship between gingival inflammation and pregnancy. | an increase in the prevalence and severity of gingival inflammation during pregnancy has been reported since the 1960s. though the etiology is not fully known, it is believed that increasing plasma sex steroid hormone levels during pregnancy have a dramatic effect on the periodontium. current works of research have shown that estrogen and progesterone increasing during pregnancy are supposed to be responsible for gingivitis progression. this review is focused not only on epidemiological studies, ... | 2015 | 25873767 |
| calculus detection technologies: where do we stand now? | epidemiological studies have implicated dental calculus as an ideal substrate for subgingival microbial colonization. therefore, the main objective of periodontal therapy is to eliminate the microbial biofilm along with the calculus deposits from the root surface by root surface debridement. over the past years, a large number of clinical and laboratory studies have been conducted to evaluate the efficacy of calculus removal by various methods. none of these conventional methods or devices was e ... | 2014 | 25870667 |
| a microbial platform for renewable propane synthesis based on a fermentative butanol pathway. | propane (c3h8) is a volatile hydrocarbon with highly favourable physicochemical properties as a fuel, in addition to existing global markets and infrastructure for storage, distribution and utilization in a wide range of applications. consequently, propane is an attractive target product in research aimed at developing new renewable alternatives to complement currently used petroleum-derived fuels. this study focuses on the construction and evaluation of alternative microbial biosynthetic pathwa ... | 2015 | 25866563 |
| construction of coa-dependent 1-butanol synthetic pathway functions under aerobic conditions in escherichia coli. | 1-butanol is an important industrial platform chemical and an advanced biofuel. while various groups have attempted to construct synthetic pathways for 1-butanol production, efforts to construct a pathway that functions under aerobic conditions have met with limited success. here, we constructed a coa-dependent 1-butanol synthetic pathway that functions under aerobic conditions in escherichia coli, by expanding the previously reported (r)-1,3-butanediol synthetic pathway. the pathway consists of ... | 2015 | 25865277 |
| beyond microbial community composition: functional activities of the oral microbiome in health and disease. | the oral microbiome plays a relevant role in the health status of the host and is a key element in a variety of oral and non-oral diseases. despite advances in our knowledge of changes in microbial composition associated with different health conditions the functional aspects of the oral microbiome that lead to dysbiosis remain for the most part unknown. in this review, we discuss the progress made towards understanding the functional role of the oral microbiome in health and disease and how nov ... | 2015 | 25862077 |
| diabetes mellitus related bone metabolism and periodontal disease. | diabetes mellitus and periodontal disease are chronic diseases affecting a large number of populations worldwide. changed bone metabolism is one of the important long-term complications associated with diabetes mellitus. alveolar bone loss is one of the main outcomes of periodontitis, and diabetes is among the primary risk factors for periodontal disease. in this review, we summarise the adverse effects of diabetes on the periodontium in periodontitis subjects, focusing on alveolar bone loss. bo ... | 2015 | 25857702 |
| pseudomonas aeruginosa and periodontal pathogens in the oral cavity and lungs of cystic fibrosis patients: a case-control study. | cystic fibrosis (cf) is the most frequent lethal genetic disease in the caucasian population. lung destruction is the principal cause of death by chronic pseudomonas aeruginosa colonization. there is a high prevalence of oropharyngeal anaerobic bacteria in sputum of cf patients. this study was carried out due to the lack of results comparing subgingival periodontal pathogenic bacteria between the oral cavity and lungs in patients with cf in relation with p. aeruginosa presence. our first goal wa ... | 2015 | 25854483 |
| proteomics - the research frontier in periodontics. | periodontitis is an inflammatory condition resulting from the interplay between the infectious agents and host factors. various protein molecules play a vital role in the initiation, progression and severity of periodontal diseases. the study of proteins as biomarkers in periodontal diseases has been highlighted during the last few years. in periodontitis multiple bacteria derived (e.g. collagen degrading enzymes, elastase like enzymes etc) and host derived mediators (eg. pge2, tnf, il1, il6, mm ... | 2015 | 25853048 |
| periodontitis and porphyromonas gingivalis in preclinical stage of arthritis patients. | to determine whether the presence of periodontitis (pd) and porphyromonas gingivalis (pg) in the subgingival biofilm associates with the development of rheumatoid arthritis (ra) in treatment naïve preclinical stage of arthritis patients. | 2015 | 25849461 |
| features of two new proteins with ompa-like domains identified in the genome sequences of leptospira interrogans. | leptospirosis is an acute febrile disease caused by pathogenic spirochetes of the genus leptospira. it is considered an important re-emerging infectious disease that affects humans worldwide. the knowledge about the mechanisms by which pathogenic leptospires invade and colonize the host remains limited since very few virulence factors contributing to the pathogenesis of the disease have been identified. here, we report the identification and characterization of two new leptospiral proteins with ... | 2015 | 25849456 |
| microbiological tests to identify a link between periodontitis and acute myocardial infarction-an original research. | gingival and periodontal diseases are associated with specific bacterial infections. the main aim of the study was to know whether the periodontitis is associated with an increased risk for acute myocardial infarction (ami) and to know the distribution of porphyromonas gingivalis in patients with acute myocardial infarction associated with chronic periodontitis and acute myocardial infarction groups. | 2013 | 25848510 |
| occurrence of periodontal pathogens in ethnic groups from a native brazilian reservation. | the present study was designed to evaluate the occurrence of periodontal pathogens in the subgingival biofilm of 100 native brazilians living at the umutina indian reservation, mato grosso state, brazil. | 2015 | 25847068 |
| outer membrane vesicles - offensive weapons or good samaritans? | outer membrane vesicles (omvs) from gram-negative bacteria were first considered as artifacts and were followed with disbelief and bad reputation. later, their existence was accepted and they became characterized as bacterial bombs, virulence bullets, and even decoys. today, we know that omvs also can be involved in cell-cell signaling/communication and be mediators of immune regulation and cause disease protection. furthermore, omvs represent a distinct bacterial secretion pathway selecting and ... | 2015 | 25840612 |
| tbx21-1993t/c (rs4794067) polymorphism is associated with increased risk of chronic periodontitis and increased t-bet expression in periodontal lesions, but does not significantly impact the ifn-g transcriptional level or the pattern of periodontophatic bacterial infection. | th1-polarized host response, mediated by ifn-γ, has been associated with increased severity of periodontal disease as well as control of periodontal infection. the functional polymorphism tbx21-1993t/c (rs4794067) increases the transcriptional activity of the tbx21 gene (essential for th1 polarization) resulting in a predisposition to a th-1 biased immune response. thus, we conducted a case-control study, including a population of healthy controls (h, n = 218), chronic periodontitis (cp, n = 197 ... | 2015 | 25832120 |
| microbiota at teeth and implants in partially edentulous patients. a 10-year retrospective study. | to determine the microbiota at implants and adjacent teeth 10 years after placement of implants with a sandblasted and acid-etched surface. | 2016 | 25827437 |
| β2-glycoprotein i-dependent anti-cardiolipin antibodies associated with periodontitis in patients with systemic lupus erythematosus. | it was reported that patients with systemic lupus erythematosus (sle) exhibited increased levels of anticardiolipin (anti-cl) antibodies, a class of antiphospholipid antibodies associated with thrombosis. β2-glycoprotein i (β2gpi) has been considered as the actual target antigen for anti-cl antibodies. this study investigates the association of periodontal infection with anti-cl antibodies in patients with sle. | 2015 | 25817824 |
| subgingival microbiota in health compared to periodontitis and the influence of smoking. | the etiology of periodontitis has traditionally been associated to a consortium of three bacterial species-the so-called "red-complex" of periodontal disease-which has been the target for most diagnostic and therapeutic strategies. however, other species have also been found to correlate with disease severity. in addition, the influence of smoking on periodontal microbiota is poorly understood. in the current manuscript, the composition of the subgingival microbiota in healthy individuals vs. pa ... | 2015 | 25814980 |
| prelude to oral microbes and chronic diseases: past, present and future. | associations between oral and systemic health are ancient. oral opportunistic bacteria, particularly, porphyromonas gingivalis and fusobacterium nucleatum, have recently been deviated from their traditional roles as periodontal pathogens and arguably ascended to central players based on their participations in complex co-dependent mechanisms of diverse systemic chronic diseases risk and pathogenesis, including cancers, rheumatoid-arthritis, and diabetes. | 2015 | 25813714 |
| periodontal therapy effects on nitrite related to oral bacteria: a 6-month randomized clinical trial. | nitrite is a biologic factor relevant to oral and systemic homeostasis. through an oral bacteria reduction process, it was suggested that periodontal therapy and chlorhexidine (chx) rinse could affect nitrite levels, leading to negative effects, such as an increase in blood pressure. this 6-month randomized clinical trial evaluated the effects of periodontal therapeutic protocols on salivary nitrite and its relation to subgingival bacteria. | 2015 | 25811847 |
| local delivery of hyaluronan 0.8% as an adjunct to scaling and root planing in the treatment of chronic periodontitis: a clinical and microbiological study. | the purpose of this study was to assess the clinical and microbiological effects of the local and sub-gingival application of a hyaluronan gel on scaling and root planing (srp) in the treatment of moderate generalized chronic periodontitis. | 2015 | 25810591 |
| antimicrobial photodynamic effect to treat residual pockets in periodontal patients: a randomized controlled clinical trial. | a randomized controlled clinical trial was designed to evaluate the efficacy of the photodynamic therapy (pdt) in the treatment of residual pockets of chronic periodontitis patients. | 2015 | 25808980 |
| subsistence strategies in traditional societies distinguish gut microbiomes. | recent studies suggest that gut microbiomes of urban-industrialized societies are different from those of traditional peoples. here we examine the relationship between lifeways and gut microbiota through taxonomic and functional potential characterization of faecal samples from hunter-gatherer and traditional agriculturalist communities in peru and an urban-industrialized community from the us. we find that in addition to taxonomic and metabolic differences between urban and traditional lifestyl ... | 2015 | 25807110 |
| bipartite topology of treponema pallidum repeat proteins c/d and i: outer membrane insertion, trimerization, and porin function require a c-terminal β-barrel domain. | we previously identified treponema pallidum repeat proteins tprc/d, tprf, and tpri as candidate outer membrane proteins (omps) and subsequently demonstrated that tprc is not only a rare omp but also forms trimers and has porin activity. we also reported that tprc contains n- and c-terminal domains (tprc(n) and tprc(c)) orthologous to regions in the major outer sheath protein (mosp(n) and mosp(c)) of treponema denticola and that tprc(c) is solely responsible for β-barrel formation, trimerization, ... | 2015 | 25805501 |
| interkingdom networking within the oral microbiome. | different sites within the oropharynx harbour unique microbial communities. co-evolution of microbes and host has resulted in complex interkingdom circuitries. metabolic signalling is crucial to these processes, and novel microbial communication factors are progressively being discovered. resolving interkingdom networks will lead to better understanding of oral health or disease aetiology. | 2015 | 25805401 |
| extract from rumex acetosa l. for prophylaxis of periodontitis: inhibition of bacterial in vitro adhesion and of gingipains of porphyromonas gingivalis by epicatechin-3-o-(4β→8)-epicatechin-3-o-gallate (procyanidin-b2-di-gallate). | the aerial parts of rumex acetosa l. have been used in traditional european medicine for inflammatory diseases of the mouth epithelial tissue. the following study aimed to investigate the influence of a proanthocyanidin-enriched extract from r. acetosa extract against the adhesion of porphyromonas gingivalis (p. gingivalis), a pathogen strongly involved in chronic and aggressive periodontitis. a further goal was to define the bioactive lead structures responsible for a potential antiadhesive act ... | 2015 | 25803708 |
| traditional uses, phytochemistry, and pharmacology of olea europaea (olive). | aim of the review. to grasp the fragmented information available on the botany, traditional uses, phytochemistry, pharmacology, and toxicology of olea europaea to explore its therapeutic potential and future research opportunities. material and methods. all the available information on o. europaea was collected via electronic search (using pubmed, scirus, google scholar, and web of science) and a library search. results. ethnomedical uses of o. europaea are recorded throughout the world where it ... | 2015 | 25802541 |
| treponemes detected in digital dermatitis lesions in brazilian dairy cattle and possible host reservoirs of infection. | the main pathogenic treponemes causing bovine digital dermatitis were identified from 17 infected herds in southern brazil for the first time in this study using pcr. we did not find a relationship between treponeme phylogroup composition and clinical classification. treponema phagedenis was present in all lesions. rumen fluid was implicated as a reservoir location for these pathogens. | 2015 | 25788552 |
| altered microbiomes in bovine digital dermatitis lesions, and the gut as a pathogen reservoir. | bovine digital dermatitis (dd) is the most important infectious disease associated with lameness in cattle worldwide. since the disease was first described in 1974, a series of treponema species concurrent with other microbes have been identified in dd lesions, suggesting a polymicrobial etiology. however, the pathogenesis of dd and the source of the causative microbes remain unclear. here we characterized the microbiomes of healthy skin and skin lesions in dairy cows affected with different sta ... | 2015 | 25781328 |
| comparative genome analysis of prevotella intermedia strain isolated from infected root canal reveals features related to pathogenicity and adaptation. | many species of the genus prevotella are pathogens that cause oral diseases. prevotella intermedia is known to cause various oral disorders e.g. periodontal disease, periapical periodontitis and noma as well as colonize in the respiratory tract and be associated with cystic fibrosis and chronic bronchitis. it is of clinical significance to identify the main drive of its various adaptation and pathogenicity. in order to explore the intra-species genetic differences among strains of prevotella int ... | 2015 | 25765460 |
| a filifactor alocis-centered co-occurrence group associates with periodontitis across different oral habitats. | periodontitis is a highly prevalent polymicrobial disease worldwide, yet the synergistic pattern of the multiple oral pathogens involved is still poorly characterized. here, saliva, supragingival and subgingival plaque samples from periodontitis patients and periodontally healthy volunteers were collected and profiled with 16s rrna gene pyrosequencing. different oral habitats harbored significantly different microbiota, and segregation of microbiota composition between periodontitis and health w ... | 2015 | 25761675 |
| effect of sumac extract on serum oxidative status, rankl/opg system and alveolar bone loss in experimental periodontitis in rats. | sumac (rhus coriaria l.) is widely used spice which has several properties such as antioxidant, anti-inflammatory and antimicrobial. the purpose of this animal study was to evaluate the effects of sumac extract on levels of receptor activator of nuclear factor-kappa b ligand (rankl), osteoprotegerin (opg) expression, serum oxidative status, and alveolar bone loss in experimental periodontitis. | 2015 | 25760266 |
| a. actinomycetemcomitans profile and red complex bacterial species of an afro-brazilian community: a comparative study. | the primary aim of this cross-sectional study was to compare the levels of red complex bacteria between afro-brazilian and non afro-brazilian cohort. the secondary aim was to compare the distribution of both aggregatibacter actinomycetemcomitans serotype b and its jp2 strains among participants who harboured this bacterial species. | 2015 | 25757149 |
| quantitative proteomics reveal distinct protein regulations caused by aggregatibacter actinomycetemcomitans within subgingival biofilms. | periodontitis is an infectious disease that causes the inflammatory destruction of the tooth-supporting (periodontal) tissues, caused by polymicrobial biofilm communities growing on the tooth surface. aggressive periodontitis is strongly associated with the presence of aggregatibacter actinomycetemcomitans in the subgingival biofilms. nevertheless, whether and how a. actinomycetemcomitans orchestrates molecular changes within the biofilm is unclear. the aim of this work was to decipher the inter ... | 2015 | 25756960 |
| high-level association of bovine digital dermatitis treponema spp. with contagious ovine digital dermatitis lesions and presence of fusobacterium necrophorum and dichelobacter nodosus. | contagious ovine digital dermatitis (codd) is an important foot disease in sheep, with significant animal welfare and economic implications. it is thought that codd emerged from bovine digital dermatitis (bdd) via treponemal bacteria. with wildlife species such as elk now suffering a codd-like disease, it is imperative to clarify these disease etiologies. a large investigation into treponemal association with codd is warranted. codd lesions (n = 58) and healthy sheep foot tissues (n = 56) were a ... | 2015 | 25740778 |
| inhibitory activity of salvadora persica extracts against oral bacterial strains associated with periodontitis: an in-vitro study. | the use of natural plant extracts in pharmacology, medicine and dental hygiene has found a growing interest in modern scientific research. salvadora persica is a natural tree whose fibrous branches have been approved by the world health organization for oral hygiene. periodontitis is a highly prevalent adult gingival disease that leads to bone destruction and connective tissue attachment loss. the aim of this research was assessment the antimicrobial activities of methanolic extract of salvadora ... | 2014 | 25737914 |
| p. gingivalis in periodontal disease and atherosclerosis - scenes of action for antimicrobial peptides and complement. | according to the nhs, it is estimated that over 50% of the adult population are, to some extent, affected by gum disease and approximately 15% of uk population have been diagnosed with severe periodontitis. periodontitis, a chronic polymicrobial disease of the gums, causes inflammation in its milder form, whereas in its severe form affects the surrounding tissues and can result in tooth loss. during periodontitis, plaque accumulates and sits between the junctional epithelium and the tooth itself ... | 2015 | 25713575 |
| periimplant bacteria associated with different transmucosal designs or smoking habits. | this study is to compare periimplant microbiota associated with implant transmucosal designs or smoking habits. | 2015 | 25706261 |
| the outer membrane protease pgte of salmonella enterica interferes with the alternative complement pathway by cleaving factors b and h. | the virulence factor pgte is an outer membrane protease (omptin) of the zoonotic pathogen salmonella enterica that causes diseases ranging from gastroenteritis to severe enteric fever. it is surface exposed in bacteria that have a short-chain, i.e., rough lps, as observed e.g., in bacteria residing inside macrophages or just emerging from them. we investigated whether pgte cleaves the complement factors b (b) and h (h), key proteins controlling formation and inactivation of the complement protei ... | 2015 | 25705210 |
| dioxygen and nitric oxide scavenging by treponema denticola flavodiiron protein: a mechanistic paradigm for catalysis. | flavodiiron proteins (fdps) contain a unique active site consisting of a non-heme diiron carboxylate site proximal to a flavin mononucleotide (fmn). fdps serve as the terminal components for reductive scavenging of dioxygen (to water) or nitric oxide (to nitrous oxide), which combats oxidative or nitrosative stress in many bacteria. characterizations of fdps from spirochetes or from any oral microbes have not been previously reported. here, we report characterization of an fdp from the anaerobic ... | 2015 | 25700637 |
| auranofin: repurposing an old drug for a golden new age. | drug discovery, development and registration is an expensive and time-consuming process associated with a high failure rate [pessetto et al. (mol cancer ther 12:1299-1309, 2013), woodcock and woosley (annu rev med 59:1-12, 2008)]. drug 'repurposing' is the identification of new therapeutic purposes for already approved drugs and is more affordable and achievable than novel drug discovery [pessetto et al. (mol cancer ther 12:1299-1309, 2013)]. auranofin is a drug that is approved for the treatmen ... | 2015 | 25698589 |
| mechanisms involved in the association between periodontitis and complications in pregnancy. | the association between periodontitis and some of the problems with pregnancy such as premature delivery, low weight at birth, and preeclampsia (pe) has been suggested. nevertheless, epidemiological data have shown contradictory data, mainly due to differences in clinical parameters of periodontitis assessment. furthermore, differences in microbial composition and immune response between aggressive and chronic periodontitis are not addressed by these epidemiological studies. we aimed to review t ... | 2015 | 25688342 |
| mechanisms involved in the association between periodontitis and complications in pregnancy. | the association between periodontitis and some of the problems with pregnancy such as premature delivery, low weight at birth, and preeclampsia (pe) has been suggested. nevertheless, epidemiological data have shown contradictory data, mainly due to differences in clinical parameters of periodontitis assessment. furthermore, differences in microbial composition and immune response between aggressive and chronic periodontitis are not addressed by these epidemiological studies. we aimed to review t ... | 2015 | 25688342 |
| [clinical and putative periodontal pathogens' features of different sites with probing depth reduction after non-surgical periodontal treatment of patients with aggressive periodontitis]. | to evaluate the differences of clinical parameters and putative periodontal pathogens in sites of different probing depth (pd) reduction after non-surgical periodontal treatment in patients with aggressive periodontitis (agp). | 2015 | 25686322 |
| inter- and intra-test agreement of three commercially available molecular diagnostic tests for the identification of periodontal pathogens. | the aim of the study was to compare the agreement and reproducibility of three test kits (iai pado test 4⋅5®, micro-ident®, and carpegen® perio diagnostik) regarding the detection of porphyromonas gingivalis, tannerella forsythia, treponema denticola, and aggregatibacter actinomycetemcomitans. | 2015 | 25680706 |
| scratching the surface - tobacco-induced bacterial biofilms. | individual environmental factors, such as iron, temperature and oxygen, are known to have a profound effect on bacterial phenotype. therefore, it is surprising so little known is about the influence of chemically complex cigarette smoke on bacterial physiology. recent evidence has demonstrated that tobacco smoke and components alter the bacterial surface and promote biofilm formation in several important human pathogens, including staphylococcus aureus, streptococcus mutans, klebsiella pneumonia ... | 2015 | 25670926 |
| non-conventional therapeutics for oral infections. | as our knowledge of host-microbial interactions within the oral cavity increases, future treatments are likely to be more targeted. for example, efforts to target a single species or key virulence factors that they produce, while maintaining the natural balance of the resident oral microbiota that acts to modulate the host immune response would be an advantage. targeted approaches may be directed at the black-pigmented anaerobes, porphyromonas gingivalis and prevotella intermedia, associated wit ... | 2015 | 25668296 |
| chronic oral infection with major periodontal bacteria tannerella forsythia modulates systemic atherosclerosis risk factors and inflammatory markers. | tannerella forsythia is a gram-negative anaerobic organism that inhabits the subgingival cavity and initiates connective tissue destruction and alveolar bone resorption in periodontal disease (pd). pd is a chronic immunoinflammatory disease and has been linked to several systemic diseases including atherosclerosis. this study evaluated the effects of a chronic oral infection with t. forsythia atcc 43037 on the induction of pd, inflammatory markers and atherosclerosis risk factors in hyperlipidem ... | 2015 | 25663343 |
| modulation of host responses by oral commensal bacteria. | immunomodulatory commensal bacteria are proposed to be essential for maintaining healthy tissues, having multiple roles including priming immune responses to ensure rapid and efficient defences against pathogens. the default state of oral tissues, like the gut, is one of inflammation which may be balanced by regulatory mechanisms and the activities of anti-inflammatory resident bacteria that modulate toll-like receptor (tlr) signalling or nf-κb activation, or influence the development and activi ... | 2015 | 25661061 |
| anti-hmuy antibodies specifically recognize porphyromonas gingivalis hmuy protein but not homologous proteins in other periodontopathogens. | given the emerging evidence of an association between periodontal infections and systemic conditions, the search for specific methods to detect the presence of p. gingivalis, a principal etiologic agent in chronic periodontitis, is of high importance. the aim of this study was to characterize antibodies raised against purified p. gingivalis hmuy protein and selected epitopes of the hmuy molecule. since other periodontopathogens produce homologs of hmuy, we also aimed to characterize responses of ... | 2015 | 25658942 |
| aggressive periodontitis: microbes and host response, who to blame? | a paradigm shift several decades ago elucidated that aggressive periodontitis (agp) was not a degenerative disorder but a rapid progressive form of plaque-induced inflammatory periodontal disease. ensuing years of research have led to linkage analysis identification of specific genetic defects responsible for agp in some families and to the finding that subgingival detection of a. actinomycet-emcomitans jp2 clone is a predictive factor for disease onset and progression. however, rather disappoin ... | 2015 | 25654663 |
| microbiome of peri-implant infections: lessons from conventional, molecular and metagenomic analyses. | osseointegrated dental implants are now a well-established treatment option in the armament of restorative dentistry. these technologically advanced devices are designed to functionally and esthetically replace missing teeth. despite the revolutionary advances that implants have incurred, they have also provided the oral cavity with new artificial surfaces prone to the formation of oral biofilms, similarly to the hard tissue surfaces of natural teeth. biofilm formation on the implant surface can ... | 2015 | 25654499 |
| relationship between invasion of the periodontium by periodontal pathogens and periodontal disease: a systematic review. | bacterial invasion of the periodontal tissues has been suggested as a relevant step in the etiopathogenesis of periodontal disease. however, its exact importance remains to be defined. the present systematic review assessed the scientific evidence concerning the relationship between the quality or quantity of periodontal microbiota in periodontal tissues and development of periodontal disease. the databases medline-pubmed, cochrane-central, isi web of knowledge and scopus were searched, up to ja ... | 2015 | 25654367 |
| photodynamic therapy as novel treatment for halitosis in adolescents: a case series study. | halitosis is a common problem that affects a large portion of the population worldwide. the origin of this condition is oral in 90% of cases and systemic in 10% of cases. the foul odor is caused mainly by volatile sulfur compounds produced by gram-negative bacteria. however, it has recently been found that anaerobic gram-positive bacteria also produce hydrogen sulfide (h2s) in the presence of amino acids, such as cysteine. light with and without the combination of chemical agents has been used t ... | 2014 | 25653814 |
| prevalence of antibiotic resistance genes in subjects with successful and failing dental implants. a pilot study. | to investigate the prevalence of the bacterial genes encoding resistance to beta-lactams, tetracyclines and metronidazole respectively, in subjects with successful and failing dental implants and to assess the presence of staphylococcus aureus and the meca gene encoding for methicillin resistant staphylococcus aureus (mrsa) in the same samples. | 2014 | 25646133 |
| prevalence of antibiotic resistance genes in subjects with successful and failing dental implants. a pilot study. | to investigate the prevalence of the bacterial genes encoding resistance to beta-lactams, tetracyclines and metronidazole respectively, in subjects with successful and failing dental implants and to assess the presence of staphylococcus aureus and the meca gene encoding for methicillin resistant staphylococcus aureus (mrsa) in the same samples. | 2014 | 25646133 |
| short-term effect of removal of fixed orthodontic appliances on gingival health and subgingival microbiota: a prospective cohort study. | the aim of this prospective longitudinal study was to assess and compare the microbiological and clinical parameters of patients wearing a fixed orthodontic appliance, as opposed to 10 days after the bracket had been removed following treatment. | 2015 | 25631494 |
| periodontopathogen levels following the use of an er:yag laser in the treatment of chronic periodontitis. | inflammatory periodontal diseases are initiated by microbial biofilms. the reduction of the biofilm is important in the management of the disease. this study compares periodontopathogen levels following the treatment of chronic periodontitis using er:yag laser debridement and mechanical scaling and root planing. | 2015 | 25630495 |
| association of the invasion ability of porphyromonas gingivalis with the severity of periodontitis. | porphyromonas gingivalis is one of the well-characterized periodontal pathogens involved in periodontitis. the invasive and proteolytic activities of p. gingivalis clinical isolates have been shown to be associated with heterogenic virulence, as determined in a mouse abscess model. the aims of the present study were to identify a p. gingivalis strain with a low virulence among clinical isolates, based on its invasive ability and cytokine proteolytic activities, and to explore the preferential de ... | 2015 | 25616643 |
| mouse genome engineering via crispr-cas9 for study of immune function. | clustered regularly interspaced palindromic repeats (crispr)-associated (cas9) technology has proven a formidable addition to our armory of approaches for genomic editing. derived from pathways in archaea and bacteria that mediate the resistance to exogenous genomic material, the crispr-cas9 system utilizes a short single guide rna (sgrna) to direct the endonuclease cas9 to virtually anywhere in the genome. upon targeting, cas9 generates dna double-strand breaks (dsbs) and facilitates the repair ... | 2015 | 25607456 |
| evidence that tp_0144 of treponema pallidum is a thiamine-binding protein. | thiamine pyrophosphate (tpp), the biologically active form of thiamine (also known as vitamin b1), is an essential cofactor for several important enzymes involved in carbohydrate metabolism, and therefore, it is required for all living organisms. we recently found that a thiamine-binding protein (tde_0143) is essential for the survival of treponema denticola, an important bacterial pathogen that is associated with human periodontitis. in this report, we provide experimental evidence showing that ... | 2015 | 25605310 |
| a 17-year old patient with dock8 deficiency, severe oral hsv-1 and aggressive periodontitis - a case of virally induced periodontitis? | we present a 17-year old girl with dock-8 deficiency, severe untreated oral hsv-1 infection and associated aggressive periodontitis. dock-8 deficiency is a primary immunodeficiency, caused by biallelicloss-of-function mutations in the dock8 gene, often leading to severe viral and fungal mucocutaneous infections. nevertheless, to date dock8 has not been associated with severe periodontitis and inflammatory bone loss around teeth. understanding whether dock8 deficiency or severe hsv-1 infection un ... | 2014 | 25600604 |
| a 17-year old patient with dock8 deficiency, severe oral hsv-1 and aggressive periodontitis - a case of virally induced periodontitis? | we present a 17-year old girl with dock-8 deficiency, severe untreated oral hsv-1 infection and associated aggressive periodontitis. dock-8 deficiency is a primary immunodeficiency, caused by biallelicloss-of-function mutations in the dock8 gene, often leading to severe viral and fungal mucocutaneous infections. nevertheless, to date dock8 has not been associated with severe periodontitis and inflammatory bone loss around teeth. understanding whether dock8 deficiency or severe hsv-1 infection un ... | 2014 | 25600604 |
| association between periodontal condition and subgingival microbiota in women during pregnancy: a longitudinal study. | in this study, the gingival conditions and the quantitative detection for aggregatibacter actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis and prevotella intermedia in pregnant women were determined. | 2014 | 25591021 |
| establishment of an oral infection model resembling the periodontal pocket in a perfusion bioreactor system. | periodontal infection involves a complex interplay between oral biofilms, gingival tissues and cells of the immune system in a dynamic microenvironment. a humanized in vitro model that reduces the need for experimental animal models, while recapitulating key biological events in a periodontal pocket, would constitute a technical advancement in the study of periodontal disease. the aim of this study was to use a dynamic perfusion bioreactor in order to develop a gingival epithelial-fibroblast-mon ... | 2015 | 25587671 |
| microbial diversity of the supra- and subgingival biofilm of healthy individuals after brushing with chlorhexidine- or silver-coated toothbrush bristles. | nanoparticulate silver has recently been reported as an effective antimicrobial agent. the aim of this clinical study was to investigate the potential changes on the oral microbiota of healthy individuals after controlled brushing with chlorhexidine- or silver-coated toothbrush bristles. twenty-four healthy participants were enrolled in this investigation and randomly submitted to 3 interventions. all the participants received, in a crossover format, the following toothbrushing interventions: (i ... | 2015 | 25581046 |
| effects of adjunctive daily phototherapy on chronic periodontitis: a randomized single-blind controlled trial. | the purpose of this randomized single-blind controlled trial was to elucidate the clinical and antimicrobial effects of daily phototherapy (pt) as an adjunct to scaling and root planing (srp) in patients with chronic periodontitis. | 2014 | 25568808 |
| association between immunoglobulin g1 against tannerella forsythia and reduction in the loss of attachment tissue. | to evaluate whether the levels of immunoglobulin g (igg) antibody to tanerella forsythia are associated with periodontal status. | 2014 | 25568807 |
| n-acetylcysteine and the human serum components that inhibit bacterial invasion of gingival epithelial cells prevent experimental periodontitis in mice. | we previously reported that human serum significantly reduces the invasion of various oral bacterial species into gingival epithelial cells in vitro. the aims of the present study were to characterize the serum component(s) responsible for the inhibition of bacterial invasion of epithelial cells and to examine their effect on periodontitis induced in mice. | 2014 | 25568806 |