Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| a serosurvey of crimean-congo haemorrhagic fever virus in dairy cattle in iran. | a seroepidemiological survey to determine the prevalence of crimean-congo haemorrhagic fever (cchf) virus among dairy cattle in iran was carried out during 2006 and 2008. blood samples were collected from 876 animals in five different provinces of the country. sera were tested by a sandwich enzyme-linked immunosorbent assay (elisa) for quantitative determination of antibodies to cchf virus. serological evidence of infection was noted in 52 (5.9%) of 876 dairy cattle. antibody prevalence increase ... | 2011 | 19912604 |
| knowledge, beliefs, and practices regarding tick bites in the turkish population in a rural area of the middle anatolian region. | the aim of this study is to determine the knowledge, beliefs, and practices of a population living in a rural area in regards to tick bites and crimean-congo hemorrhagic fever (cchf). the study was conducted in a rural area located in central anatolia in the region of eskişehir. a total of 1,500 individuals aged 20 years and older chosen by a stratified random sample were enrolled. a questionnaire was administered in person. in 264 (17.4%) participants, there was a history of being bitten by a t ... | 2010 | 19882230 |
| the trend towards habitat fragmentation is the key factor driving the spread of crimean-congo haemorrhagic fever. | we aimed to characterize an environmental niche driving the distribution of crimean-congo haemorrhagic fever (cchf) in turkey, using a geo-referenced collection of cases reported between 2003 and 2008 and a set of climate and vegetation features. we used mean monthly air temperatures and normalized derived vegetation index (ndvi) values, at a resolution of 0.1 degrees , as well as climate features at and below the surface. we computed significant differences in monthly variables between positive ... | 2010 | 19878611 |
| crimean-congo hemorrhagic fever virus-encoded ovarian tumor protease activity is dispensable for virus rna polymerase function. | crimean-congo hemorrhagic fever virus (cchfv) is a tick-borne virus (genus nairovirus, family bunyaviridae) associated with high case fatality disease outbreaks in regions of africa, europe, and asia. the cchfv genome consists of three negative-strand rna segments, s, m, and l. the unusually large virus l polymerase protein and the need for biosafety level 4 (bsl-4) containment conditions for work with infectious virus have hampered the study of cchfv replication. the l protein has an ovarian tu ... | 2010 | 19864393 |
| emergence of crimean-congo haemorrhagic fever in greece. | in the summer of 2008, the first case of crimean-congo haemorrhagic fever (cchf) was observed in greece. the laboratory diagnosis was established using nested rt-pcr and quantitative real-time rt-pcr. a high viral load and increased levels of cytokines were detected on the third day of illness and the patient died 7 days after the onset of symptoms. nucleotide sequence analysis revealed that the greek cchf virus strain had high sequence identity with other balkan cchf virus strains. | 2010 | 19845692 |
| [crimean-congo haemorrhagic fever: an enemy at the gates]. | crimea-congo haemorrhagic fever is a tick-borne viral zoonosis with the potential of human-to-human transmission. the disease occurs in extensive areas in asia, south-eastern europe and africa. haemorrhagic manifestations constitute a prominent symptom of the late disease stage, with case fatality rates from 9 to 50%. the recent increase in the number of cases in eastern europe and the potential for nosocomial outbreaks indicate the advisability of diagnosis in every patient hospitalized in ital ... | 2009 | 19838084 |
| crimean-congo hemorrhagic fever virus as a nosocomial pathogen in iran. | crimean-congo hemorrhagic fever (cchf) is a viral disease with several different modes of transmission. we describe the manifestations, outcome, and likely modes of transmission for three nosocomial cases. all three cases were healthcare workers (two men and one woman). they had fever, myalgia, and petechia. disseminated intravascular coagulation resulted in the death occurred in the woman. because this disease is manifested with non-specific influenza-like symptoms, diagnosis can be difficult. ... | 2009 | 19815885 |
| differential activation profiles of crimean-congo hemorrhagic fever virus- and dugbe virus-infected antigen-presenting cells. | crimean-congo hemorrhagic fever virus (cchfv) is a highly pathogenic, tick-borne member of the family bunyaviridae and the genus nairovirus. to better elucidate the pathogenesis of cchfv, we analysed the host innate immune response induced in antigen-presenting cells (apcs) infected in vitro by cchfv. monocyte-derived dendritic cells (dcs) and macrophages (mps) were both shown to be permissive for cchfv and to replicate the virus, as monitored by genomic and antigenomic strand quantification. vi ... | 2010 | 19812268 |
| towards an understanding of the migration of crimean-congo hemorrhagic fever virus. | crimean-congo haemorrhagic fever (cchf) is a lethal disease caused by crimean-congo hemorrhagic fever virus (cchfv). it is one of the most widespread medically significant tick-borne pathogens, with a distribution that coincides well with the geographical occurrence of its tick vector, hyalomma marginatum marginatum. sporadic outbreaks of cchf have previously been recognized in asia, africa, the middle east and europe but, in the 21st century, outbreaks have become more frequent in former yugosl ... | 2010 | 19812264 |
| pregnancy and crimean-congo haemorrhagic fever. | crimean-congo hemorrhagic fever (cchf) is a potentially fatal viral infection with reported case fatality rates of 5-30%. humans become infected through tick bites, by contact with a patient with cchf during the acute phase of infection, or by contact with blood or tissues from viraemic livestock. in this first report in the literature, we present the characteristics of three pregnant women with cchf infection and the outcome of their babies. transmission of the cchf infection could be either in ... | 2010 | 19778302 |
| the frequency of self-reported allergic diseases in patients with crimean-congo haemorrhagic fever. | crimean-congo haemorrhagic fever (cchf) is an acute, tick-borne viral disease. in temperate areas, cchf cases occur between spring and early autumn when tick activity is high. this period is also the pollen season during which symptoms of allergic diseases are exacerbated. viruses induce inflammatory and antiviral responses by binding to specific receptors on the surface of airway epithelial cells, resulting in activation of innate immune responses; release of mediators such as cytokines and che ... | 2009 | 19775796 |
| [two cases of crimean-congo haemorrhagic fever (cchf) in two tourists in senegal in 2004]. | two cases of crimean-congo haemorrhagic fever (cchf) occurred in two french tourists during their visit in senegal in november 2004. febrile and hemorrhagic syndrome with ulorrhagia, petechiae, haematemesis, haematomas associated with biological signs of disseminated intramuscular coagulation were observed. for the first case who had a medical evacuation to france before diagnosis, crimean-congo virus infection was revealed by laboratory tests performed by the national reference center for hemor ... | 2009 | 19739410 |
| the interferon response circuit in antiviral host defense. | viruses have learned to multiply in the face of a powerful innate and adaptive immune response of the host. they have evolved multiple strategies to evade the interferon (ifn) system which would otherwise limit virus growth at an early stage of infection. ifns induce the synthesis of a range of antiviral proteins which serve as cell-autonomous intrinsic restriction factors. for example, the dynamin-like mxa gtpase inhibits the multiplication of influenza and bunyaviruses (such as la crosse virus ... | 2009 | 19739399 |
| detection of crimean-congo hemorrhagic fever virus genome in saliva and urine. | the crimean-congo hemorrhagic fever (cchf) virus is transmitted by tick bites and by contact with the blood or tissues of infected patients and livestock. this study was designed to investigate the genome of cchf virus in saliva and urine samples of patients with cchf. | 2010 | 19656706 |
| assessing the impact of climate change on vector-borne viruses in the eu through the elicitation of expert opinion. | expert opinion was elicited to undertake a qualitative risk assessment to estimate the current and future risks to the european union (eu) from five vector-borne viruses listed by the world organization for animal health. it was predicted that climate change will increase the risk of incursions of african horse sickness virus (ahsv), crimean-congo haemorrhagic fever virus (cchfv) and rift valley fever virus (rvfv) into the eu from other parts of the world, with african swine fever virus (asfv) a ... | 2010 | 19580695 |
| crimean congo hemorrhagic fever virus infects human monocyte-derived dendritic cells. | for some patients infection with crimean congo hemorrhagic fever virus (cchfv) causes a severe disease characterized by fever, vascular leakage and coagulopathy. knowledge of cchf pathogenesis is limited and today there is no information about the specific target cells of cchfv. in this study we analyzed the permissiveness of human peripheral blood mononuclear cells (pbmcs) including monocyte-derived dendritic cells (modcs) to cchfv infection. interestingly, we found that modcs are the most perm ... | 2009 | 19570561 |
| evolution of crimean-congo hemorrhagic fever virus. | the evolutionary patterns of each genome rna segment of crimean-congo hemorrhagic fever virus (cchfv) were investigated. nucleotide sequences from 40 cchfv strains isolated during time period spanning from 1956 to 2003 were tested. it was found that there is not any global molecular clock ticking at the same rate for cchfv strains; however a local clock is applicable in s rna segment among strains in the individual clades. african strains seem to evolve faster than strains from other geographic ... | 2009 | 19560561 |
| epidemiology and phylogenetic analysis of crimean-congo hemorrhagic fever viruses in xinjiang, china. | in 2004 and 2005, an epidemiological survey of crimean-congo hemorrhagic fever virus (cchfv) was conducted in xinjiang, china. a total of 5,629 serum samples of human and livestock were collected and tested for the cchfv antibody, and 17,319 ticks were collected for viral identification. reverse passive hemagglutination inhibition assays showed that the average prevalence of cchfv antibody was 1.7% for the humans and 12.7% for the livestock. a relatively high antibody prevalence, ranging from 19 ... | 2009 | 19553586 |
| crimean-congo hemorrhagic fever in southeastern europe. | crimean-congo hemorrhagic fever (cchf) is an acute, tick-borne viral disease, affecting only humans and newborn mice, with hemorrhagic manifestations and considerable mortality in humans. cchf virus circulates in nature in an enzootic tick-vertebrate-tick cycle; migrating birds and livestock transferred from endemic to non-endemic areas may carry large numbers of infected ticks thus spreading the cchf virus into novel areas. from 2000 through 2008, the infection emerged or re-emerged in bulgaria ... | 2009 | 19540787 |
| crimean-congo hemorrhagic fever, southwestern bulgaria. | 2009 | 19523314 | |
| the first clinical case due to ap92 like strain of crimean-congo hemorrhagic fever virus and a field survey. | crimean-congo hemorrhagic fever (cchf) is a fatal infection, but no clinical case due to ap92 strain was reported. we described the first clinical case due to ap92 like cchfv. | 2009 | 19515251 |
| [crimean-congo hemorrhagic fever in senegal]. | 2009 | 19499725 | |
| non-infectious plasmid engineered to simulate multiple viral threat agents. | the aim of this study was to design and construct a non-virulent simulant to replace several pathogenic viruses in the development of detection and identification methods in biodefense. a non-infectious simulant was designed and engineered to include the nucleic acid signature of veev (venezuelan equine encephalitis virus), influenza virus, rift valley fever virus, machupo virus, lassa virus, yellow fever virus, ebola virus, eastern equine encephalitis virus, junin virus, marburg virus, dengue v ... | 2009 | 19442841 |
| an outbreak of crimean-congo hemorrhagic fever in western anatolia, turkey. | sporadic crimean-congo hemorrhagic fever (cchf) cases were first reported in turkey in 2002, arising particularly in northeastern anatolia. epidemics have been reported in neighboring countries since the 1970s. with the increase in number of cchf virus infected or suspected cases in the aydin region of western anatolia by 2006, we decided to focus attention on this disease. | 2009 | 19409826 |
| ecology of the crimean-congo hemorrhagic fever endemic area in albania. | crimean-congo hemorrhagic fever virus (cchfv) is endemic in albania. ticks collected from cattle grazing in the endemic areas of albania were tested for presence of cchfv rna, while serum samples collected from goats, cattle, hares, and birds were tested for the presence of specific igg antibodies to cchfv. one of the 31 pools prepared, consisting of four female hyalomma spp. ticks, was found to carry cchfv rna with 99.2-100% homology to sequences detected in patients from the same region. antib ... | 2009 | 19402760 |
| international network for capacity building for the control of emerging viral vector-borne zoonotic diseases: arbo-zoonet. | arboviruses are arthropod-borne viruses, which include west nile fever virus (wnfv), a mosquito-borne virus, rift valley fever virus (rvfv), a mosquito-borne virus, and crimean-congo haemorrhagic fever virus (cchfv), a tick-borne virus. these arthropod-borne viruses can cause disease in different domestic and wild animals and in humans, posing a threat to public health because of their epidemic and zoonotic potential. in recent decades, the geographical distribution of these diseases has expande ... | 2009 | 19341603 |
| efficacy of oral ribavirin treatment in crimean-congo haemorrhagic fever: a quasi-experimental study from turkey. | the aim of this study was to evaluate the efficacy of oral ribavirin treatment in patients with crimean-congo haemorrhagic fever (cchf). | 2009 | 19246100 |
| crimean-congo hemorrhagic fever virus in high-risk population, turkey. | in the tokat and sivas provinces of turkey, the overall crimean-congo hemorrhagic fever virus (cchfv) seroprevalence was 12.8% among 782 members of a high-risk population. cchfv seroprevalence was associated with history of tick bite or tick removal from animals, employment in animal husbandry or farming, and being >40 years of age. | 2009 | 19239765 |
| low-density macroarray for rapid detection and identification of crimean-congo hemorrhagic fever virus. | crimean-congo hemorrhagic fever (cchf) is a tick-borne viral zoonosis which occurs throughout africa, eastern europe, and asia and results in an approximately 30% fatality rate. a reverse transcription-pcr assay including a competitive internal control was developed on the basis of the most up-to-date genome information. biotinylated amplification products were hybridized to dna macroarrays on the surfaces of polymer supports, and hybridization events were visualized by incubation with a strepta ... | 2009 | 19225100 |
| ocular findings in patients with crimean-congo hemorrhagic fever. | to describe ocular findings of patients with crimean-congo hemorrhagic fever (cchf). | 2009 | 19195635 |
| a case of crimean-congo hemorrhagic fever with pleural effusion. | crimean-congo hemorrhagic fever (cchf) is a tick-borne viral zoonosis with the potential of human-to-human transmission that affects wide areas in asia, southeastern europe, and africa. hemorrhagic manifestations constitute a prominent symptom of late stage disease with case fatality rates from 3 to 50%. we present a case of cchf complicated by hemorrhagic pleural effusion and resulting in resolution without chest tube drainage in a 9-year-old boy. the diagnosis of cchf was confirmed by enzyme-l ... | 2009 | 19168966 |
| evaluation of the efficacy of ribavirin therapy on survival of crimean-congo hemorrhagic fever patients: a case-control study. | the mortality rate resulting from crimean-congo hemorrhagic fever (cchf) has been reported in different epidemics to range from 5% to more than 70%. while ribavirin has been recommended as the drug of choice in the treatment of cchf, no consistent study has unequivocally demonstrated its effectiveness. using the case-control method, we attempted to evaluate the efficacy of ribavirin in reducing mortality among cchf cases admitted to boo-ali educational hospital in zahedan, iran, during the years ... | 2009 | 19168952 |
| genetic relationship in southern african crimean-congo haemorrhagic fever virus isolates: evidence for occurrence of reassortment. | crimean-congo haemorrhagic fever (cchf) is a tick-borne viral zoonosis widely distributed in africa, asia and eastern europe. reassortment of cchf genome segments has been shown to occur in nature. we therefore investigated the genetic relationship of southern african isolates using partial sequence data for each rna segment, s, m and l, and comparing the tree topologies constructed using a neighbour joining method. a total of 21 southern african isolates were studied. the incongruencies which w ... | 2009 | 19161643 |
| crimean-congo hemorrhagic fever in greece: a public health perspective. | in june 2008 the first non-imported fatal case of crimean-congo hemorrhagic fever (cchf) was recorded in northern greece. we present herein the public health interventions and the case definitions we developed for the epidemiological investigation. the possibility of cchf establishing endemicity in this area is discussed. | 2009 | 19155182 |
| microtubule-dependent and microtubule-independent steps in crimean-congo hemorrhagic fever virus replication cycle. | following binding and entry many viruses exploit the host cell cytoskeleton to ensure intracellular transport, assembly or egress. for crimean-congo hemorrhagic fever virus (cchfv), the causative agent of a severe hemorrhagic disease, virus-host interactions are poorly investigated. in this study we demonstrated that drug-induced suppression of microtubule dynamics and especially microtubule disassembly, impaired cchfv biogenesis. our results showed that intact microtubules were required early d ... | 2009 | 19150104 |
| analysis of lymphocyte subgroups in crimean-congo hemorrhagic fever. | this study examined the association between lymphocyte subgroups and mortality in patients with crimean-congo hemorrhagic fever (cchf) in turkey. | 2009 | 19112036 |
| crimean-congo hemorrhagic fever virus entry and replication is clathrin-, ph- and cholesterol-dependent. | to date, the entry pathway and replication mechanisms for members of the family bunyaviridae, and especially for crimean-congo hemorrhagic fever virus (cchfv), are poorly understood. considering the severity of disease and the widespread geographical occurrence of cchfv, investigating viral entry is of great value for development of antivirals. in this study, we have shown that knockdown of clathrin by small interfering rna significantly reduced cchfv nucleocapsid protein and viral rna levels, s ... | 2009 | 19088291 |
| the risk of transmission of crimean-congo hemorrhagic fever virus from human cases to first-degree relatives. | to determine the infectivity of crimean-congo hemorrhagic fever (cchf) virus via routine contacts between serologically confirmed cases and their close relatives from may 2005 up to march 2006, 79 serum samples of 57 close relatives of 12 newly diagnosed serologically confirmed cchf cases in the sistan-va-baluchestan province of iran were tested for igg and igm antibodies against cchf virus using the enzyme-linked immunosorbent assay technique. nine levels of contacts were considered: percutaneo ... | 2008 | 19050365 |
| interferon and cytokine responses to crimean congo hemorrhagic fever virus; an emerging and neglected viral zonoosis. | crimean congo hemorrhagic fever virus (cchfv) causes an acute disease with the potential of a fatal outcome. the virus is prevalent in about 30 countries. clinical symptoms of infection commonly include fever, myalgia, and hemorrhages. levels of liver enzymes are raised, and bleeding markers are often increased. a role of inflammatory cytokines in the pathogenesis has been suggested, and cchfv employs a range of passive and active mechanisms to avoid induction of the antiviral type i interferons ... | 2008 | 19027345 |
| a case of crimean-congo haemorrhagic fever in greece, june 2008. | 2008 | 18761893 | |
| a preliminary report on crimean-congo haemorrhagic fever in turkey, march - june 2008. | 2008 | 18761892 | |
| a preliminary study to evaluate the effect of intravenous ribavirin treatment on survival rates in crimean-congo hemorrhagic fever. | 2008 | 18722669 | |
| an early warning system for crimean-congo haemorrhagic fever seasonality in turkey based on remote sensing technology. | in the last few years, crimean-congo haemorrhagic fever (cchf) has been reported as an emerging tickborne disease in turkey. this paper deals with the preparation of an early warning system, aimed to predict the beginning of the cchf season in turkey based on a clear, simple and repeatable remotely-sensed signal. decadal (mean of 10 days) values of the normalized difference vegetation index (ndvi) at 1 km resolution were used on a set of 952 confirmed, accurately geo-referenced, clinical cases b ... | 2007 | 18686262 |
| surveillance and laboratory detection system of crimean-congo haemorrhagic fever in iran. | crimean-congo haemorrhagic fever (cchf) is a viral zoonotic disease with a high mortality rate in humans. the cchf virus (cchfv) is transmitted to humans through the bite of ixodid ticks or by contact with blood or tissues of infected livestock. in addition to zoonotic transmission, cchfv can be spread from person to person and is one of the rare haemorrhagic fever viruses able to cause nosocomial outbreaks in hospitals. crimean-congo haemorrhagic fever is a public health problem in many regions ... | 2008 | 18666963 |
| suspected crimean congo haemorrhagic fever cases in albania. | during 2003 to 2006 samples from 34 albanian patients with suspected crimean congo hemorrhagic fever (cchf) were tested by serology and pcr for cchf virus; negative samples were further tested for hantaviruses, leptospira spp. and rickettsia spp. cchf virus was detected in 38.2% of cases, hantaviruses in 11.7%, and leptospirosis and rickettsiosis were diagnosed in 29.4% and 2.9% of cases, respectively. there is a seasonal and clinical overlapping among the 4 diseases in albania, suggesting that ... | 2008 | 18609200 |
| crimean-congo hemorrhagic fever virus delays activation of the innate immune response. | as a first line of defence against virus infection, mammalian cells elicit an innate immune response, characterized by secretion of type i interferons and the up-regulation of interferon stimulated genes. many viruses down-regulate the innate immune responses in order to enhance their virulence. crimean-congo hemorrhagic fever virus (cchfv), a nairovirus of the family bunyaviridae is the causative agent of severe hemorrhagic fever in humans with high mortality. knowledge regarding the innate imm ... | 2008 | 18551619 |
| processing of genome 5' termini as a strategy of negative-strand rna viruses to avoid rig-i-dependent interferon induction. | innate immunity is critically dependent on the rapid production of interferon in response to intruding viruses. the intracellular pathogen recognition receptors rig-i and mda5 are essential for interferon induction by viral rnas containing 5' triphosphates or double-stranded structures, respectively. viruses with a negative-stranded rna genome are an important group of pathogens causing emerging and re-emerging diseases. we investigated the ability of genomic rnas from substantial representative ... | 2008 | 18446221 |
| peripheral blood natural killer cells in crimean-congo hemorrhagic fever. | the relationship of changes in the peripheral blood lymphocyte subgroups during crimean-congo hemorrhagic fever (cchf) to prognosis has not been reported. | 2008 | 18434247 |
| knowledge and attitude toward crimean-congo haemorrhagic fever in occupationally at-risk iranian healthcare workers. | we assessed the knowledge and attitude (k&a) toward crimean-congo haemorrhagic fever (cchf) of occupationally at-risk healthcare workers (hcws). a cross-sectional survey was performed in three referral hospitals in the systan-baluchestan and isfahan provinces of iran where cchf is highly endemic. in all, 191/209 eligible hcws were enrolled (response rate: 93%). all but 11 (5.8%) had heard of cchf. the mean k&a scores of the respondents were 50.34% and 79.25%, respectively. the correlation betwee ... | 2008 | 18395295 |
| synthesis of 1-beta-d-ribofuranosyl-3-ethynyl-[1,2,4]triazole and its in vitro and in vivo efficacy against hantavirus. | there are no fda approved drugs for the treatment of hemorrhagic fever with renal syndrome (hfrs), a serious human illnesses caused by hantaviruses. clinical studies using ribavirin (rbv) to treat hfrs patients suggest that it provides an improved prognosis when given early in the course of disease. given the unique antiviral activity of rbv and the lack of other lead scaffolds, we prepared a diverse series of 3-substituted 1,2,4-triazole-beta-ribosides and identified one with antiviral activity ... | 2008 | 18394724 |
| [in vitro activity of russian ribavirin on the models of crimean-congo hemorrhagic fever virus, rift valley fever virus, and tahyna and dhori viruses]. | the high activity of ribavirin made by effective biotechnology in russia was established in in vitro experiments using the models crimean-congo hemorrhagic fever virus, rift valley fever virus, and tahyna and dhori viruses, which suggests that it is promising in using the drug in the treatment of infection with these viruses. | 2008 | 18318134 |
| [respiratory lesions in congo-crimean hemorrhagic fever]. | to characterize pathogenesis, clinical, laboratory and x-ray features of respiratory disease in congo-crimean hemorrhagic fever (cchf). | 2007 | 18219967 |
| viral load as predictor of crimean-congo hemorrhagic fever outcome. | we used quantitative real-time reverse transcription-pcr to measure viral load in serum from 24 patients in kosovo who had acute crimean-congo hemorrhagic fever. viral load correlated with clinical disease and antibodies and could be used as a predictor of disease outcome. | 2007 | 18217568 |
| virus detection and monitoring of viral load in crimean-congo hemorrhagic fever virus patients. | we developed a real-time reverse transcription--pcr that detected 1,164 copies/ml of crimean-congo hemorrhagic fever virus per milliliter of serum at 95% probability (probit analysis) and was 100% concordant with nested pcr on 63 samples from 31 patients with confirmed infection. infected patients who died appeared to have higher viral loads; low viral loads correlated with igg detection. | 2007 | 18214191 |
| the complete genome sequence of a crimean-congo hemorrhagic fever virus isolated from an endemic region in kosovo. | the balkan region and kosovo in particular, is a well-known crimean-congo hemorrhagic fever (cchf) endemic region, with frequent epidemic outbreaks and sporadic cases occurring with a hospitalized case fatality of approximately 30%. recent analysis of complete genome sequences of diverse cchf virus strains showed that the genome plasticity of the virus is surprisingly high for an arthropod-borne virus. high levels of nucleotide and amino acid differences, frequent rna segment reassortment and ev ... | 2008 | 18197964 |
| treatment of crimean-congo hemorrhagic fever. | crimean-congo hemorrhagic fever (cchf) has the most extensive geographic range of the medically significant tick-borne viruses, occurring from western china across southern asia to eastern europe and south africa. the causative agent is a negative-sense, single-stranded rna virus in the genus nairovirus, family bunyaviridae. in published reports, the case fatality rate has generally ranged from 10% to 50%. sporadic cases and outbreaks of the disease have increased during the past decade across t ... | 2008 | 18096251 |
| modeling the spatial distribution of crimean-congo hemorrhagic fever outbreaks in turkey. | the more than 1100 human cases of crimean-congo hemorrhagic fever (cchf) reported in a continuing series of outbreaks that started in 2003 in turkey constitute the largest epidemic of the disease since cchf was first recognized in 1944. the spatial distribution of cchf case reporting rates in turkey was studied to look for clusters of disease. we applied the spatial scan analysis to test the hypothesis of whether there were areas with a higher than expected number of cchf cases. the analysis was ... | 2007 | 18047397 |
| viral load and crimean-congo hemorrhagic fever. | 2007 | 18044055 | |
| genetic analysis of the m rna segment of crimean-congo hemorrhagic fever virus strains in turkey. | crimean-congo hemorrhagic fever (cchf) virus is member of the genus nairovirus of the family bunyaviridae. all members of the family bunyaviridae are enveloped viruses containing tripartite, negative polarity, single-stranded rna. cchf is characterized by high case mortality, occurring in asia, africa, the middle east and europe. during recent years, outbreaks have been reported in turkey. however, little information is available on the genetic diversity of cchf virus in turkey. in this study, a ... | 2008 | 17955162 |
| detection of a questing hyalomma marginatum marginatum adult female (acari, ixodidae) in southern germany. | the first finding of a questing hyalomma marginatum marginatum female tick in germany is described. the tick was found in may 2006 on the clothing of a person who had spent the preceding day in rural surroundings in southern germany. as the infested person had also been visiting spain where h. m. marginatum is known to occur some weeks prior to finding the tick, it is not clear whether the tick had been imported by him as a female or by another host in a preimaginal stage and succeeded to develo ... | 2007 | 17952610 |
| breastfeeding in crimean-congo haemorrhagic fever. | crimean-congo haemorrhagic fever (cchf) is potentially a fatal disease transmitted by tick bite, close contact with blood or tissues of infected humans or viraemic livestock. we present the clinical course of 2 breastfeeding women with cchf and their babies. both the mothers had positive reverse transcriptase-polymerase chain reaction for cchf virus in blood and it was negative in breast milk. at follow-up, babies did not develop cchf infection. | 2008 | 17934982 |
| crimean-congo hemorrhagic fever virus glycoprotein processing by the endoprotease ski-1/s1p is critical for virus infectivity. | crimean-congo hemorrhagic fever virus (cchfv) causes severe human disease. the cchfv medium rna encodes a polyprotein which is proteolytically processed to yield the glycoprotein precursors pregn and pregc, followed by structural glycoproteins gn and gc. subtilisin kexin isozyme-1/site-1 protease (ski-1/s1p) plays a central role in gn processing. here we show that cchfv-infected cells deficient in ski-1/s1p produce no infectious virus, although pregn and pregc accumulated normally in the golgi a ... | 2007 | 17898072 |
| development of a taqman minor groove binding protein assay for the detection and quantification of crimean-congo hemorrhagic fever virus. | crimean-congo hemorrhagic fever virus (cchfv) is a tick-borne virus of the genus nairovirus and the family bunyaviridae. it is a negative-strand rna virus comprised of small (s), medium (m), and large (l) genome segments. the s segment encodes for nucleocapsid protein, the m segment codes for envelope glycoproteins (gn and gc), and the l segment codes for the rna-dependent rna polymerase. currently, there are a limited number of methods for rapidly diagnosing cchfv infections. we developed a rea ... | 2007 | 17827370 |
| viral load as a predictor of outcome in crimean-congo hemorrhagic fever. | crimean-congo hemorrhagic fever (cchf) is a potentially fatal disease affecting multiple organ systems. to determine the association between viral load and severity of cchf infection, quantitative measurement of cchf virus was performed using 1-step reverse-transcriptase polymerase chain reaction for 36 patients with cchf infection. viral loads ranged from 1.1x10(3) copies/ml to > or = 9.9x10(9) copies/ml. nine (25%) of 36 patients died. in 8 of the 9 patients with fatal outcomes, viral loads we ... | 2007 | 17806044 |
| crimean-congo hemorrhagic fever. | crimean-congo hemorrhagic fever is a tick-borne viral zoonosis with the potential of human-to-human transmission, affecting wide areas in asia, southeastern europe, and africa. hemorrhagic manifestations constitute a prominent symptom of late stage disease with case fatality rates from 10% to 50%. the purpose of this article is to review recent literature on crimean-congo hemorrhagic fever with emphasis on its epidemiology and management. | 2007 | 17762783 |
| [elisa and pcr test systems used to detect crimean-congo hemorrhagic fever virus]. | studying the sensitivity and specificity of enzyme-linked immunosorbent assay (elisa) for the indication of crimean-congo hemorrhagic fever (cchf) virus antigens and those of reverse transcription and polymerase chain reaction (rt-pcr) for the detection of cchf virus rna, and those of a intercerebral infection method in newborn albino mice systems for the determination of viral infectious activity established that the sensitivity of elisa was 1-2 orders of magnitude less than that of rp-pcr. the ... | 2007 | 17722612 |
| clinical investigation of the transient evoked otoacoustic emission test in crimean-congo hemorrhagic fever. | the aim of this study was to investigate cochlear damage in crimean-congo hemorrhagic fever (cchf) infection. | 2008 | 17714968 |
| recent progress in molecular biology of crimean-congo hemorrhagic fever. | crimean-congo hemorrhagic fever (cchf) is a severe hemorrhagic fever in humans with a case fatality rate of up to 50%. a causative agent of cchf is cchf virus, which is a tick-borne virus in the family bunyaviridae, genus nairovirus. the virus is transmitted to humans through infected tick bites, squashed ticks or from direct contact with viremic animals or humans. outbreaks of cchf have been documented in africa, the middle east, eastern europe and western asia where the vector and/or reservoir ... | 2007 | 17692916 |
| haemophagocytosis in a patient with crimean congo haemorrhagic fever. | crimean-congo haemorrhagic fever (cchf) is a severe disease with a case fatality of 2.8 to 80 %. a patient dwelling in an endemic region for cchf was admitted with fever preceding bleeding diathesis and pancytopenia. despite no history of tick exposure, cchf was highly suspected. with an oral ribavirin therapy, clinical and laboratory improvements were obtained. the diagnosis was confirmed by detection of igm antibody to cchf virus and positive rt-pcr. although the main pathogenesis of cchf infe ... | 2007 | 17644726 |
| imported crimean-congo hemorrhagic fever cases in istanbul. | we described a series of imported cases of crimean-congo hemorrhagic fever (cchf) in istanbul and investigated the genetic diversity of the virus. all the suspected cases of cchf, who were applied to the health centers in istanbul, were screened for cchf virus (cchfv) infection by using semi-nested polymerase chain reaction (pcr) following rt-pcr. simultaneous blood samples were also sent to the national reference laboratory in ankara for serologic investigation. in 10 out of 91 patients, cchfv ... | 2007 | 17553137 |
| [crimean-congo hemorrhagic fever]. | 2007 | 17491361 | |
| crimean-congo haemorrhagic fever cases in turkey. | crimean-congo haemorrhagic fever (cchf) is an arbovirus infection, which is transmitted through ticks or via blood and secretions. until recently, human cases of cchf were unknown in turkey; however, several acute disease cases were reported in 2002. we report on the investigation of a cluster of suspected cchf cases in the middle part of the black sea from may 2002 to october 2003. the medical charts that we reviewed were obtained from all local physicians and our field investigations. 'suspect ... | 2007 | 17454897 |
| emerging zoonoses and vector-borne infections affecting humans in europe. | the purpose of this study was to assess and describe the current spectrum of emerging zoonoses between 2000 and 2006 in european countries. a computerized search of the medline database from january 1966 to august 2006 for all zoonotic agents in european countries was performed using specific criteria for emergence. fifteen pathogens were identified as emerging in europe from 2000 to august 2006: rickettsiae spp., anaplasma phagocytophilum, borrelia burgdorferi, bartonella spp., francisella tula ... | 2007 | 17445320 |
| [geography and host distribution of crimean-congo hemorrhagic fever in the tarim basin]. | to determine the infective status and natural distribution of xinjiang hemorrhagic fever (xhf; crimean-congo hemorrhagic fever, cchf) in ticks, rodents and livestock in the tarim basin. | 2006 | 17415983 |
| identification of a novel c-terminal cleavage of crimean-congo hemorrhagic fever virus pregn that leads to generation of an nsm protein. | the structural glycoproteins of crimean-congo hemorrhagic fever virus (cchfv; genus nairovirus, family bunyaviridae) are derived through endoproteolytic cleavage of a 1,684-amino-acid m rna segment-encoded polyprotein. this polyprotein is cotranslationally cleaved into the pregn and pregc precursors, which are then cleaved by ski-1 and a ski-1-like protease to generate the n termini of gn and gc, respectively. however, the resulting polypeptide defined by the n termini of gn and gc is predicted ... | 2007 | 17409136 |
| crimean-congo hemorrhagic fever. | crimean-congo hemorrhagic fever is a tick-borne viral disease reported from more than 30 countries in africa, asia, south-east europe, and the middle east. the majority of human cases are workers in livestock industry, agriculture, slaughterhouses, and veterinary practice. nosocomial transmission is also well described. clinical manifestations are nonspecific and symptoms typically include high fever, headache, malaise, arthralgia, myalgia, nausea, abdominal pain, and nonbloody diarrhea. patient ... | 2007 | 17367225 |
| crimean-congo hemorrhagic fever among health care workers in iran: a seroprevalence study in two endemic regions. | crimean-congo hemorrhagic fever (cchf) has repeatedly caused nosocomial outbreaks among hospital staff. in the summer of 2003, we studied the seroprevalence of anti-cchf igg among health care workers who had come in contact with crimean-congo hemorrhagic fever patients from three referral hospitals in endemic regions of iran. a total of 223 eligible staff were examined. whereas 5 of 129 (3.87%) exposed health care workers tested positive, none of the 94 in the unexposed group did (p=0.075). sero ... | 2007 | 17360865 |
| [molecular analysis on the s gene of three crimean-congo hemorrhagic fever virus strains in china]. | to compare the molecular characteristics of 3 crimean-congo hemorrhagic fever viruses(cchfv) isolated in xinjiang province. | 2006 | 17343183 |
| ex vivo stability of the rodent-borne hantaan virus in comparison to that of arthropod-borne members of the bunyaviridae family. | the possible effect of virus adaptation to different transmission routes on virus stability in the environment is not well known. in this study we have compared the stabilities of three viruses within the bunyaviridae family: the rodent-borne hantavirus hantaan virus (htnv), the sand fly-borne phlebovirus sandfly fever sicilian virus (sfsv), and the tick-borne nairovirus crimean-congo hemorrhagic fever virus (cchfv). these viruses differ in their transmission routes: sfsv and cchfv are vector bo ... | 2007 | 17337567 |
| isolation of crimean-congo hemorrhagic fever virus from ticks on imported sudanese sheep in saudi arabia. | 2000 | 17322717 | |
| n-linked glycosylation of gn (but not gc) is important for crimean congo hemorrhagic fever virus glycoprotein localization and transport. | the mature gn glycoprotein of crimean congo hemorrhagic fever (cchf) virus contains two predicted glycosylation sites (557n and 755n). of these, n-glycans are added only at 557n, as evidenced by abrogation of gn-glycosylation by mutation of 557n but not 755n site. mutational block of gn-glycosylation at 557n did not significantly affect gn proteolytic processing but did result in mislocalization and retention of gn and other proteins synthesized from the virus m segment orf (gp160, gp85, gp38 an ... | 2007 | 17197010 |
| basolateral entry and release of crimean-congo hemorrhagic fever virus in polarized mdck-1 cells. | crimean-congo hemorrhagic fever virus (cchfv) is an etiological agent of a disease with mortality rates in patients averaging 30%. the disease is characterized by fever, myalgia, and hemorrhage. mechanisms underlying the hemorrhage have to our knowledge not been elucidated for cchfv. possibly, a direct or indirect viral effect on tight junctions (tj) could cause the hemorrhage observed in patients, as tj play a crucial role in vascular homeostasis and can cause leakage upon deregulation. moreove ... | 2007 | 17166898 |
| [characterization of the natural foci of congo-crimean hemorrhagic fever on the territory of ukraine]. | the natural foci of the congo-crimean hemorrhagic fever (cchf) in ukraine were characterized by their geographical factors. the circulation of cchf virus was found to occur mainly in forest and forest-steppe zones irrespective of the location of administrative regions. the territories found to be most favorable for the prolonged existence of the focus of cchf were those having high humidity. the possibility of using geoinformation technologies for the correction of the boundaries of natural foci ... | 2006 | 17163141 |
| crimean-congo hemorrhagic fever virus: genetic analysis and tick survey in turkey. | crimean-congo hemorrhagic fever virus (cchfv) is a tick-borne virus in the family bunyaviridae, genus nairovirus. the virus is transmitted to humans through infected tick bites or from direct contact with viremic animals or humans. in the present study, a total of 1,015 adult ticks were collected from cattle (603 specimens), sheep (17 specimens), and goats (395 specimens) in the kelkit valley in turkey. four tick species were recognized on the animals in the surveyed region. the most abundant sp ... | 2006 | 17088370 |
| lookback exercise with imported crimean-congo hemorrhagic fever, senegal and france. | a patient with suspected malaria was hospitalized successively in 2 hospitals, first in dakar, senegal, then in rennes, france, where tests diagnosed crimean-congo hemorrhagic fever. an international incident management group was set up in france and senegal, which traced 181 contacts and analyzed 50 samples from 3 countries. no secondary cases were identified clinically. | 2006 | 17073094 |
| seroprevalence of crimean-congo hemorrhagic fever in sistan-va-baluchestan province of iran. | during the years 2000 to 2004, of 248 serologically confirmed cases of crimean-congo hemorrhagic fever (cchf) that occurred in several parts of iran, 169 were reported from sistan-va-baluchestan province. to assess the seroprevalence of cchf virus infection within the zahedan and zabol districts of the sistan-va-baluchestan province in iran, 300 subjects were sampled from the general population. in addition to blood sampling, a questionnaire was completed for every subject. all but just 3 of our ... | 2006 | 17060701 |
| global warming may increase the incidence and geographic range of crimean-congo hemorrhagic fever. | 2007 | 17056187 | |
| crimean-congo haemorrhagic fever in kosova : a fatal case report. | crimean-congo haemorrhagic fever (cchf) is an often fatal viral infection described in about 30 countries around the world. the authors report a fatal case of crimean-congo hemorrhagic fever (cchf) observed in a patient from kosova. the diagnosis of cchf was confirmed by reverse transcription-pcr. late diagnosis decreased the efficacy of treatment and patient died due to severe complications of infection. | 2006 | 17034648 |
| [crimean-congo hemorrhagic fever]. | crimean-congo hemorrhagic fever (cchf) virus is a tick-borne virus, which is a member of bunyaviridae family, nairovirus genus. cchf virus has been isolated from 31 different tick species so far, and genus hyalomma includes the basic vectors of which h. anatolicum, h. marginatum, h. detritum, h. dromedarii, h. excavatum and h. turanicum are frequently found in the geography in which turkey takes place. the virus is transmitted via the bite of infected ticks or direct contact with cchf infected p ... | 2006 | 17001860 |
| zoonotic infections among veterinarians in turkey: crimean-congo hemorrhagic fever and beyond. | we aimed to determine the seroprevalence of crimean-congo hemorrhagic fever (cchf) virus, brucella spp, and coxiella burnetii among veterinarians in a highly endemic and a non-endemic region for these infections in turkey. | 2006 | 16978897 |
| recombinant expression, purification, and kinetic and inhibitor characterisation of human site-1-protease. | human site-1-protease (s1p, merops s08.8063), also widely known as subtilisin/kexin isozyme 1 (ski-1), is a membrane bound subtilisin-related serine protease, that belongs to a group of nine mammalian proprotein convertases. among these proteases, s1p displays unique substrate specificity, by showing preferred cleavage after non-basic amino acids. s1p plays a key role in a proteolytic pathway that controls the cholesterol content of membranes, cells and blood. s1p also participates in the activa ... | 2007 | 16973377 |
| crimean-congo hemorrhagic fever virus genomics and global diversity. | crimean-congo hemorrhagic fever (cchf) is a severe illness with high case fatality that occurs in africa, europe, the middle east, and asia. the complete genomes of 13 geographically and temporally diverse virus strains were determined, and cchf viruses were found to be highly variable with 20 and 8%, 31 and 27%, and 22 and 10% nucleotide and deduced amino acid differences detected among virus s (nucleocapsid), m (glycoprotein), and l (polymerase) genome segments, respectively. distinct geograph ... | 2006 | 16912331 |
| [genomic s segment of crimean-congo hemorrhagic fever virus circulating in russia and bulgaria]. | s-segment nucleotide sequences for two crimean-congo hemorrhagic fever (cchf) virus strains isolated in the rostov region of russia and in bulgaria have been determined. analysis of complete s-segment nucleotide sequences in the viral strains from different regions of the world has established that the cchf virus strains isolated from ticks and human beings in different southern russian regions in 1967 and 2000 are very closely genetically and they form an individual subgroup in the basic europe ... | 2006 | 16826753 |
| the complete genomic sequence of strain ros/huvlv-100, a representative russian crimean congo hemorrhagic fever virus strain. | the complete genomic sequence (minus primer-generated ends) of the laboratory-adapted crimean congo hemorrhagic fever virus (cchfv) strain ros/huvlv-100, isolated in 2003 from the blood of a deceased female from the rostov region of southern european russia, was determined by direct sequencing of overlapping reverse transcription/polymerase chain reaction amplified products. the size of the ros/huvlv-100 genome is 19.2 kilobases--individual genome segments are similar in size and sequence featur ... | 2006 | 16791423 |
| cytokine levels in crimean-congo hemorrhagic fever. | crimean-congo hemorrhagic fever (cchf) virus causes severe disease in humans with a mortality reaching 30%. a cchf outbreak took place in albania in 2003. as in other viral hemorrhagic fevers cytokines may be involved and play a role in the pathogenesis and outcome of the disease. | 2006 | 16765637 |
| [evaluation of the probability of the infection of medical personnel with crimean-congo hemorrhagic fever virus in cases of introduction of the infective agent to a non-endemic territory of russia]. | the data on the secondary cases of infection with crimean-congo hemorrhagic fever (chf) virus during the period of 1960 - 2002 have been analyzed and the probability of the infection of medical personnel with this virus has been evaluated. the data obtained in this study may be used in the development of the mathematical model of chf epidemics (outbreaks), taking into account not only the transmission of the infective agent, but also hospital infection, as well as for calculating the number medi ... | 2006 | 16758893 |
| [genetic monitoring of the crimean-congo hemorrhagic fever virus in kazakhstan and tajikistan in 2001-2003]. | blood specimens obtained from 32 cchf patients were tested for the presence of cchf virus markers. in addition, 3210 ticks of the genera hyalomma asiaticum, hyalomma anatolicum, and dermacentor niveus were examined to identify the cchf virus antigen and rna. this material was obtained during the 2001-2003 local outbreaks of cchf in kazakhstan and tajikistan. the nucleotide sequence in the region 983-1282 of s segment of the cchf virus for 12 wild type strains was determined. the phylogenetic rel ... | 2006 | 16756002 |
| crimean-congo haemorrhagic fever presenting as thrombotic microangiopathy and acute renal failure. | 2006 | 16735392 | |
| analysis of risk-factors among patients with crimean-congo haemorrhagic fever virus infection: severity criteria revisited. | the aim of this study was to determine the predictors of mortality among patients infected with crimean-congo haemorrhagic fever (cchf) virus. among patients with acute febrile syndrome, characterised by malaise, bleeding, leukopenia and thrombocytopenia, who were admitted to hospital during the spring and summer of 2002-2004, 54 had positive igm and/or pcr results for cchf virus in blood or tissue. the overall case fatality rate was 7.4%. among the fatalities, haematemesis (p 0.009), melaena (p ... | 2006 | 16700704 |
| exogenous nitric oxide inhibits crimean congo hemorrhagic fever virus. | crimean congo hemorrhagic fever virus (cchfv) is a geographically widespread pathogen that causes severe hemorrhagic fever with high mortality. even though one of the main objectives focuses on the progress of antiviral agents, the research on cchfv is strongly hampered due to its bsl-4 classification. nitric oxide (no), a mediator with broad biological effects, has been shown to possess inhibitory properties against various pathogens. the molecule constitutes a component of the innate immunity ... | 2006 | 16632039 |