Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| interferon-╬│ and proliferation responses to salmonella enterica serotype typhi proteins in patients with s. typhi bacteremia in dhaka, bangladesh. | salmonella enterica serotype typhi is a human-restricted intracellular pathogen and the cause of typhoid fever. cellular immune responses are required to control and clear salmonella infection. despite this, there are limited data on cellular immune responses in humans infected with wild type s. typhi. | 2011 | 21666798 |
| a retrospective study of post-mortem examination findings in takahe (porphyrio hochstetteri). | to identify the major diseases or disease processes affecting both captive and wild populations of takahe (porphyrio hochstetteri) in birds submitted for post-mortem examination between 1992 and 2007, and to survey archived wild dead takahe for the presence of erysipelothrix rhusiopathiae. | 2011 | 21660844 |
| the effects of brazilian and bulgarian propolis in vitro against salmonella typhi and their synergism with antibiotics acting on the ribosome. | salmonella enterica serovar typhi is the causative agent of typhoid fever in humans, and the use of antibiotics is essential for controlling this infection; however, the excessive use of antibiotics may select resistant strains. propolis is a honeybee product and its antimicrobial activity has been intensively investigated. thus, the objective of this study was to investigate a possible synergism between propolis (collected in brazil and bulgaria) and antibiotics acting on the ribosome (chloramp ... | 2011 | 21660841 |
| outbreak of salmonella enterica serotype infantis producing arma 16s rna methylase and ctx-m-15 extended-spectrum +¦-lactamase in a neonatology ward in constantine, algeria. | plasmid-mediated 16s rrna methylases such as arma, which confer high levels of resistance to aminoglycosides, are increasingly reported in enterobacteriaceae. this study investigated the molecular mechanism of +¦-lactam and aminoglycoside resistance in extended-spectrum +¦-lactamase (esbl)-producing salmonella enterica serotype infantis isolated at the 53-bed neonatology ward of university hospital benabib in constantine, algeria. from september 2008 to january 2009, 200 s. enterica isolates wer ... | 2011 | 21658916 |
| inverse regulation in the metabolic genes pcka and mete revealed by proteomic analysis of the salmonella rcscdb regulon. | the rcsc, rcsd, and rcsb proteins compose a system used by enteric bacteria to sense envelope stress. signal transmission occurs from the sensor rcsc to the transcriptional regulator rcsb. accessory proteins, such as igaa, are known to adjust the response level. in a previous transcriptomic study, we uncovered 85 genes differentially expressed in salmonella enterica serovar typhimurium igaa mutants. here, we extended these observations to proteomics by performing differential isotope-coded prote ... | 2011 | 21657791 |
| an infrequent molecular ruler controls flagellar hook length in salmonella enterica. | the bacterial flagellum consists of a long external filament connected to a membrane-embedded basal body at the cell surface by a short curved structure called the hook. in salmonella enterica, the hook extends 55 nm from the cell surface. flik, a secreted molecular ruler, controls hook length. upon hook completion, flik induces a secretion-specificity switch to filament-type substrate secretion. here, we demonstrate that an infrequent ruler mechanism determines hook length. flik is intermittent ... | 2011 | 21654632 |
| chromosomal rearrangements in salmonella enterica serovar typhi strains isolated from asymptomatic human carriers. | host-specific serovars of salmonella enterica often have large-scale chromosomal rearrangements that occur by recombination between rrn operons. two hypotheses have been proposed to explain these rearrangements: (i) replichore imbalance from horizontal gene transfer drives the rearrangements to restore balance, or (ii) the rearrangements are a consequence of the host-specific lifestyle. although recent evidence has refuted the replichore balance hypothesis, there has been no direct evidence for ... | 2011 | 21652779 |
| enhancing the role of veterinary vaccines reducing zoonotic diseases of humans: linking systems biology with vaccine development. | the aim of research on infectious diseases is their prevention, and brucellosis and salmonellosis as such are classic examples of worldwide zoonoses for application of a systems biology approach for enhanced rational vaccine development. when used optimally, vaccines prevent disease manifestations, reduce transmission of disease, decrease the need for pharmaceutical intervention, and improve the health and welfare of animals, as well as indirectly protecting against zoonotic diseases of people. ... | 2011 | 21651944 |
| the expression levels of outer membrane proteins stm1530 and ompd, which are influenced by the cpxar and baesr two-component systems, play important roles in the ceftriaxone resistance of salmonella enterica serovar typhimurium. | significant increases in stm3031, stm1530 and acrd protein levels and significant decreases in ompc and ompd protein levels are present when the ceftriaxone-resistant s. enterica serovar typhimurium r200 strain is compared with the ceftriaxone-susceptible strain 01-4. acrd is known to be involved in drug export and stm3031 seems to play a key role in ceftriaxone resistance. here, we examine the role of stm1530, ompc and ompd in ceftriaxone resistance. an ompd gene deletion mutant showed a 4-fold ... | 2011 | 21646491 |
| the pmrcab operon mediates polymyxin resistance in acinetobacter baumannii atcc 17978 and clinical isolates through phosphoethanolamine modification of lipid a. | the emergence of multidrug resistance amongst acinetobacter baumannii is leading to an increasing dependence on the use of polymyxins as last hope antibiotics. here we utilized genetic and biochemical methods to define the involvement of the pmrcab operon in polymyxin resistance in this organism. sequence analysis of 16 polymyxin b resistant strains, including six spontaneous mutants derived from strain atcc 17978 and 10 clinical isolates from diverse sources, revealed that they had independent ... | 2011 | 21646482 |
| prevalence and counts of salmonella spp. in minimally processed vegetables in são paulo, brazil. | minimally processed vegetables (mpv) may be important vehicles of salmonella spp. and cause disease. this study aimed at detecting and enumerating salmonella spp. in mpv marketed in the city of são paulo, brazil. a total of 512 samples of mpv packages collected in retail stores were tested for salmonella spp. and total coliforms and escherichia coli as indication of the hygienic status. salmonella spp. was detected in four samples, two using the detection method and two using the counting method ... | 2011 | 21645825 |
| molecular characterization of salmonella enterica serovar saintpaul isolated from imported seafood, pepper, environmental and clinical samples. | a total of 39 salmonella enterica serovar saintpaul strains from imported seafood, pepper and from environmental and clinical samples were analyzed for the presence of virulence genes, antibiotic resistance, plasmid and plasmid replicon types. pulsed-field gel electrophoresis (pfge) fingerprinting using the xbai restriction enzyme and plasmid profiling were performed to assess genetic diversity. none of the isolates showed resistance to ampicillin, chloramphenicol, gentamicin, kanamycin, strepto ... | 2011 | 21645810 |
| multi-comparative systems biology analysis reveals time-course biosignatures of in vivo bovine pathway responses to b.melitensis, s.enterica typhimurium and m.avium paratuberculosis. | to decipher the complexity and improve the understanding of host-pathogen interactions, biologists must adopt new system level approaches in which the hierarchy of biological interactions and dynamics can be studied. this paper presents the application of systems biology for the cross-comparative analysis and interactome modeling of three different infectious agents, leading to the identification of novel, unique and common molecular host responses (biosignatures). | 2011 | 21645321 |
| gene expression pattern in swine neutrophils after lipopolysaccharide exposure: a time course comparison. | experimental exposure of swine neutrophils to bacterial lipopolysaccharide (lps) represents a model to study the innate immune response during bacterial infection. neutrophils can effectively limit the infection by secreting lipid mediators, antimicrobial molecules and a combination of reactive oxygen species (ros) without new synthesis of proteins. however, it is known that neutrophils can modify the gene expression after lps exposure. we performed microarray gene expression analysis in order t ... | 2011 | 21645290 |
| the salmonella enterica pan-genome. | salmonella enterica is divided into four subspecies containing a large number of different serovars, several of which are important zoonotic pathogens and some show a high degree of host specificity or host preference. we compare 45 sequenced s. enterica genomes that are publicly available (22 complete and 23 draft genome sequences). of these, 35 were found to be of sufficiently good quality to allow a detailed analysis, along with two escherichia coli strains (k-12 substr. dh10b and the avian p ... | 2011 | 21643699 |
| tubal factor infertility is associated with antibodies against chlamydia trachomatis heat shock protein 60 (hsp60) but not human hsp60. | background serum antibodies against major outer membrane protein (momp) and heat shock protein 60 (hsp60) from chlamydia trachomatis are correlated with sequelae following infection. since bacterial and human hsp60 share considerable sequence homology, cross-reactivity to human hsp60 is suggested as being involved in tubal factor infertility (tfi). the aim was to investigate whether antibodies to human hsp60 are associated with tfi, and to evaluate antibody testing in tfi diagnosis. methods seru ... | 2011 | 21642639 |
| structure-guided investigation of lipopolysaccharide o-antigen chain length regulators reveals regions critical for modal length control. | the o-antigen component of the lipopolysaccharide (lps) represents a population of polysaccharide molecules with a non-random (modal) chain length distribution. the number of the repeat o-units in each individual o-antigen polymer depends on the wzz chain-length regulator, an inner membrane protein belonging to the polysaccharide co-polymerase (pcps) family. different wzz proteins confer vastly different ranges of modal lengths (4 to >100 repeat units) despite having remarkably conserved structu ... | 2011 | 21642455 |
| decay of bacterial pathogens, fecal indicators, and real-time quantitative pcr genetic markers in manure amended soils. | this study examined persistence and decay of bacterial pathogens, fecal indicator bacteria (fib), and emerging real-time quantitative pcr (qpcr) genetic markers for rapid detection of fecal pollution in manure-amended agricultural soils. known concentrations of transformed green fluorescent protein-expressing escherichia coli o157:h7/pzs and red fluorescent protein-expressing salmonella enterica serovar typhimurium/pds were added to laboratory-scale manure-amended soil microcosms with moisture c ... | 2011 | 21642395 |
| characterization of the carbohydrate backbone of vibrio parahaemolyticus o6 lipopolysaccharides. | structural characterization studies have been carried out on the carbohydrate backbone of vibrio parahaemolyticus serotype o6 lipopolysaccharides (lps). the carbohydrate backbone isolated from o6 lps by sequential derivatization, i.e., dephosphorylation, o-deacylation, pyridylamination, n-deacylation and n-acetylation, is a nonasaccharide consisting of 3 mol of d-glucosamine (glcn) (of which one is pyridylaminated), 2 mol of l-glycero-d-manno-heptose (hep), and 1 mol each of d-galactose (gal), d ... | 2011 | 21639862 |
| crystallization and preliminary x-ray crystallographic analysis of salmonellatyphimurium cuep. | salmonella enterica serovar typhimurium (s. typhimurium) can survive in the phagosome of macrophages, causing serious medical and veterinary problems. cuep is uniquely found in s. typhimurium and has been characterized as a major periplasmic copper-binding protein. although cuep has been identified as being responsible for the copper resistance of the bacterium in vivo, the biochemical role and three-dimensional structure of cuep remain unknown. in this study, cuep from s. typhimurium was overex ... | 2011 | 21636909 |
| the relative efficacy of different strain combinations of lactic acid bacteria in the reduction of populations of salmonella enterica typhimurium in the livers and spleens of mice. | multispecies probiotics have been reported to be more effective than monostrain probiotics in health promoting for the host. in this study, twelve lactic acid bacteria (lab) strains were selected based on the level of induction of tnf-α in raw 264.7 macrophage cells. their adherence to caco-2 cells and inhibitory effects on salmonella invasion of caco-2 cells were compared. strains with different probiotic properties were then combined and balb/c mice were fed with lab strains for 63 days, then ... | 2011 | 21635568 |
| synthesis, characterization and biological properties of thienyl derived triazole schiff bases and their oxovanadium(iv) complexes. | a new series of biologically active thienyl derived triazole schiff bases and their oxovanadium(iv) complexes have been synthesized and characterized on the basis of physical (m.p., magnetic susceptibility and conductivity), spectral (ir, (1)h and (13)c nmr, electronic and mass spectrometry) and microanalytical data. all the schiff base ligands and their oxovanadium(iv) complexes have been subjected to in vitro antibacterial activity against four gram-negative (escherichia coli, shigella flexner ... | 2011 | 21635212 |
| the intestinal microbiota plays a role in salmonella-induced colitis independent of pathogen colonization. | the intestinal microbiota is composed of hundreds of species of bacteria, fungi and protozoa and is critical for numerous biological processes, such as nutrient acquisition, vitamin production, and colonization resistance against bacterial pathogens. we studied the role of the intestinal microbiota on host resistance to salmonella enterica serovar typhimurium-induced colitis. using multiple antibiotic treatments in 129s1/svimj mice, we showed that disruption of the intestinal microbiota alters h ... | 2011 | 21633507 |
| salmonella synthesizing 1-monophosphorylated lipopolysaccharide exhibits low endotoxic activity while retaining its immunogenicity. | the development of safe live, attenuated salmonella vaccines may be facilitated by detoxification of its lps. recent characterization of the lipid a 1-phosphatase, lpxe, from francisella tularensis allowed us to construct recombinant, plasmid-free strains of salmonella that produce predominantly 1-dephosphorylated lipid a, similar to the adjuvant approved for human use. complete lipid a 1-dephosphorylation was also confirmed under low ph, low mg(2+) culture conditions, which induce lipid a modif ... | 2011 | 21632711 |
| bacteriophage selection against a plasmid-encoded sex apparatus leads to the loss of antibiotic-resistance plasmids. | antibiotic-resistance genes are often carried by conjugative plasmids, which spread within and between bacterial species. it has long been recognized that some viruses of bacteria (bacteriophage; phage) have evolved to infect and kill plasmid-harbouring cells. this raises a question: can phages cause the loss of plasmid-associated antibiotic resistance by selecting for plasmid-free bacteria, or can bacteria or plasmids evolve resistance to phages in other ways? here, we show that multiple antibi ... | 2011 | 21632619 |
| survival of salmonella newport in oysters. | salmonella enterica is the leading cause of laboratory-confirmed foodborne illness in the united states and raw shellfish consumption is a commonly implicated source of gastrointestinal pathogens. a 2005 epidemiological study done in our laboratory by brands et al., showed that oysters in the united states are contaminated with salmonella, and in particular, a specific strain of the newport serovar. this work sought to further investigate the host-microbe interactions between salmonella newport ... | 2011 | 21632135 |
| prenatal exposure to lps leads to long-lasting physiological consequences in male offspring. | growing evidence suggests that early life events are critical determinants for disorders later in life. according to a comprehensive number of epidemiological/animal studies, exposure to lipopolysaccharide, causes alteration in pro-inflammatory cytokine levels, hypothalamic-pituitary-adrenal functioning and the hormonal system which may contribute to behavioral and neurological injuries. in this study we investigated the effects of lipopolysaccharide administration on physiological parameters in ... | 2011 | 21630247 |
| in vitro activity of azithromycin in salmonella isolates from pakistan. | enteric fever is caused by salmonella enterica serovars typhi and paratyphi a, b and c. it is a significant public health issue in pakistan, which is exacerbated by a high level of resistance some isolates display to drugs routinely used in treatment. azithromycin may be a treatment option for such isolates. | 2011 | 21628817 |
| salmonella enterica in semi-aquatic turtles in colombia. | turtles can be hosts of salmonella enterica serovars which can cause disease both in the animals themselves and in people they come into contact with, especially when the turtles are kept as pets. to investigate the prevalence of salmonella in turtles in colombia, we studied animals at a wildlife protection centre. the turtles had either been confiscated or donated to the centre. | 2011 | 21628812 |
| multidrug-resistant typhoid fever: a review. | multidrug-resistant typhoid fever (mdrtf) is defined as typhoid fever caused by salmonella enterica serovar typhi strains (s. typhi), which are resistant to the first-line recommended drugs for treatment such as chloramphenicol, ampicillin and trimethoprim-sulfamethoxazole. since the mid-1980s, mdrtf has caused outbreaks in several countries in the developing world, resulting in increased morbidity and mortality, especially in affected children below five years of age and those who are malnouris ... | 2011 | 21628808 |
| expression of tlr4 and downstream effectors in selected cecal cell sub-populations of chicks resistant or susceptible to salmonella carrier-state. | toll-like receptor 4 (tlr4), which recognizes lipopolysaccharide from gram negative bacteria plays a major role in resistance of mice and humans to salmonella infection. in chicken, salmonella may establish a carrier-state whereby bacteria are able to persist in the organism without triggering clinical signs. based on cellular morphological parameters, we developed a method, without using antibodies, to separate three cecal cell sub-populations: lymphocytes, enterocytes, and a population encompa ... | 2011 | 21628520 |
| antibodies in action: the role of human opsonins in killing salmonella enterica serovar typhi. | although vaccines have been available for over a century, a correlate of protection for typhoid fever has yet to be identified. antibodies are produced in response to typhoid infection and vaccination, and are generally used as the gold standard for determining vaccine immunogenicity even though their role in clearance of s. typhi infections is poorly defined. herein, we describe the first functional characterization of s. typhi-specific antibodies following vaccination with a new vaccine, m01zh ... | 2011 | 21628517 |
| β-glucan plus ascorbic acid in neonatal calves modulates immune functions with and without salmonella enterica serovar dublin. | to determine if β-glucan plus ascorbic acid affects adherence and pathogenicity of salmonella dublin and innate immune response in neonatal calves, 20 calves were fed control or supplemented diets (β-glucan, 0.9g/d, plus ascorbic acid, 500mg/d) until d 23. on d 21, 5 calves per treatment received 2.4×10(8)cfu of s. dublin orally. s. dublin spread through intestinal tissues into mesenteric lymph nodes (mln), spleen, and lung tissues within 48h. all supplemented calves had less mrna expression of ... | 2011 | 21628075 |
| the salmonella enterica phop directly activates the horizontally acquired spi-2 gene ssel and is functionally different from a s. bongori ortholog. | to establish a successful infection within the host, a pathogen must closely regulate multiple virulence traits to ensure their accurate temporal and spatial expression. as a highly adapted intracellular pathogen, salmonella enterica has acquired during its evolution various virulence genes via numerous lateral transfer events, including the acquisition of the salmonella pathogenicity island 2 (spi-2) and its associated effectors. beneficial use of horizontally acquired genes requires that their ... | 2011 | 21625519 |
| humanized mice for salmonella typhi infection: new tools for an old problem. | salmonella enterica serovar typhi (s. typhi), is a human restricted pathogen and the causal agent of typhoid fever. although the use of antimicrobial drugs or vaccines has served as an effective therapeutics strategy against typhoid fever, the recent surge in multidrug resistant strains of s. typhi presents a major health concern worldwide. progress on typhoid research has been limited in the past due to the lack of a suitable animal model that recapitulates the hallmark immunological features o ... | 2011 | 21623167 |
| complete genome sequence of the universal killer, salmonella enterica serovar typhimurium uk-1 (atcc 68169). | the salmonella enterica serovar typhimurium strain uk-1 presents the highest invasion and virulence attributes among the most frequently studied strains. s. typhimurium uk-1 has been used as the foundation for developing recombinant vaccines and been extensively used on virulence and colonization studies in chickens and mice. we describe here the complete genome sequence of s. typhimurium uk-1. comparative genomics of salmonella typhimurium will provide insight into factors that determine virule ... | 2011 | 21622747 |
| phosphorylation of the autophagy receptor optineurin restricts salmonella growth. | selective autophagy can be mediated via receptor molecules that link specific cargoes to the autophagosomal membranes decorated by ubiquitin-like lc3 (microtubule-associated protein light chain 3) modifiers. although several autophagy receptors have been identified, little is known about mechanisms controlling their functions in vivo. here, we found that phosphorylation of an autophagy receptor, optineurin, promoted selective autophagy of ubiquitin-coated cytosolic salmonella enterica. the prote ... | 2011 | 21617041 |
| mouse models to assess the efficacy of non-typhoidal salmonella vaccines: revisiting the role of host innate susceptibility and routes of challenge. | non-typhoidal salmonella enterica (nts) serovars typhimurium and enteritidis are important causes of bacterial gastroenteritis in the usa and worldwide. in sub-saharan africa these two serovars are emerging as agents associated with lethal invasive disease (e.g., bacteremia, meningitis). the development of nts vaccines, based on mucosally administered live attenuated strains and parenteral non-living antigens, could diminish the nts disease burden globally. mouse models of s. typhimurium and s. ... | 2011 | 21616112 |
| impact of a probiotic fermented milk in the gut ecosystem and in the systemic immunity using a non-severe protein-energy-malnutrition model in mice. | abstract: background: malnutrition affects the immune response, causing a decrease of defence mechanisms and making the host more susceptible to infections. probiotics can reconstitute the intestinal mucosa and stimulate local and systemic immunity. the aim of this work was evaluate the effects of a probiotic fermented milk as a complement of a re-nutrition diet, on the recovery of the intestinal barrier, and mucosal and systemic immune functions in a murine model of non-severe protein-energy-ma ... | 2011 | 21615956 |
| salmonella genomic island 1-j variants associated with change in the antibiotic resistance gene cluster in multidrug-resistant salmonella enterica serovar virchow isolated from humans, taiwan, 2004-2006. | clin microbiol infect abstract: salmonella genomic island 1 (variant sgi1-j3) has been previously identified in multi-drug resistant (mdr) salmonella enterica serovar virchow isolated from humans in 1994. in this study, antimicrobial resistance, genotypes and genetic relationship were investigated in 96 s. virchow isolates collected from humans in 2004-2006. xbai-pfge analysis separated 96 isolates into two main related clusters, i and ii, which consisted of four major pulsotypes differing in pr ... | 2011 | 21615827 |
| babies and bearded dragons: sudden increase in reptile-associated salmonella enterica serovar tennessee infections, germany 2008. | abstract introduction: in 2008 a marked increase in salmonella enterica serovar tennessee infections in infants occurred in germany. in march and april 2008, eight cases were notified compared to a median of 0-1 cases in 2001-2006. materials and methods: we carried out an investigation including a case-control study to identify the source of infection. a patient was a child <3 years of age with salmonella tennessee isolated from stool from september 1, 2007, through december 31, 2008, identified ... | 2011 | 21612527 |
| mycobacterium avium subsp. avium and salmonella enterica serotype typhimurium var. copenhagen phage type dt2 in pigeons. | abstract we report on a coinfection of mycobacterium avium subsp. avium and salmonella enterica serotype typhimurium var. copenhagen phage type dt2 in pigeons from one flock, from which squabs were occasionally consumed by humans. triplex quantitative real-time pcr and culture methods were used for m. a. avium detection in livers and culture method was used for the detection of salmonella sp. in samples of liver and caecum of 33 examined birds. m. a. avium was detected in a total of 31 (93.9%) a ... | 2011 | 21612426 |
| molecular epidemiological investigation of a diffuse outbreak caused by salmonella enterica serotype montevideo isolates in osaka prefecture, japan. | abstract in osaka prefecture, japan, three foodborne outbreaks were caused by salmonella enterica serotype montevideo in rapid succession between september 2007 and may 2008. further, salmonella montevideo was also isolated from several sporadic diarrhea patients and asymptomatic carriers examined during approximately the identical period. to investigate the relatedness of the isolates, we performed antimicrobial susceptibility testing, pulsed-field gel electrophoresis (pfge) analysis, and multi ... | 2011 | 21612424 |
| comprehensive analysis of salmonella sequence polymorphisms and development of a ldr-ua assay for the detection and characterization of selected serotypes. | salmonella is a major cause of food-borne disease, and salmonella enterica subspecies i includes the most clinically relevant serotypes. salmonella serotype determination is important for the disease etiology assessment and contamination source tracking. this task will be facilitated by the disclosure of salmonella serotype sequence polymorphisms, here annotated in seven genes (sefa, safa, safc, biga, inva, fima, and phsb) from 139 s. enterica strains, of which 109 belonging to 44 serotypes of s ... | 2011 | 21611799 |
| a method to generate recombinant salmonella typhi ty21a strains expressing multiple heterologous genes using an improved recombineering strategy. | live attenuated salmonella enterica serovar typhi ty21a (ty21a) is an important vaccine strain used in clinical studies for typhoid fever and as a vaccine vector for the expression of heterologous antigens. to facilitate the use of ty21a in such studies, it is desirable to develop improved strategies that enable the stable chromosomal integration and expression of multiple heterologous antigens. the phage λ red homologous recombination system has previously been used in various gram-negative bac ... | 2011 | 21611798 |
| allb, allantoin utilisation and salmonella enterica serovar enteritidis and typhimurium colonisation of poultry and mice. | natural variation in the presence or the absence of stm0517-0529 genes allowing allantoin utilisation has been described in field isolates of the multidrug resistant salmonella enterica serovar typhimurium belonging to the phage type dt104. interestingly, s. enterica subspecies enterica serovar typhimurium dt104 is quite frequent in pigs and cattle, but rarely present in egg-laying hens. taking into account the different mode of allantoin metabolism in birds and mammals, we were interested in wh ... | 2011 | 21611691 |
| the spectrin cytoskeleton is crucial for adherent and invasive bacterial pathogenesis. | various enteric bacterial pathogens target the host cell cytoskeletal machinery as a crucial event in their pathogenesis. despite thorough studies detailing strategies microbes use to exploit these components of the host cell, the role of the spectrin-based cytoskeleton has been largely overlooked. here we show that the spectrin cytoskeleton is a host system that is hijacked by adherent (entropathogenic escherichia coli [epec]), invasive triggering (salmonella enterica serovar typhimurium [s. ty ... | 2011 | 21603579 |
| the long-term persistence of blacmy-2 positive, inca/c plasmids requires selection pressure. | multidrug resistance (mdr), bla(cmy-2) plasmids that confer resistance to third-generation cephalosporins have been found in multiple bacterial species collected from different hosts worldwide. the widespread distribution of bla(cmy-2) plasmids may be driven by antibiotic use that selects for the dissemination and persistence of these plasmids. alternatively, these plasmids may persist and spread in bacterial populations in the absence of selection pressure if a balance exists between conjugativ ... | 2011 | 21602382 |
| comparative genomics of 28 salmonella enterica isolates: evidence for crispr-mediated adaptive sublineage evolution. | despite extensive surveillance, foodborne salmonella enterica infections continue to cause a significant burden on public health systems worldwide. as the s. enterica species comprises sublineages with greatly differing antigenic representation, virulence and antimicrobial resistance phenotypes, a better understanding of the species evolution is critical for the prediction and prevention of future outbreaks. the role that virulence and resistance phenotype acquisition, exchange and loss play for ... | 2011 | 21602358 |
| a shigella boydii bacteriophage which resembles salmonella phage vii. | abstract: background: lytic bacteriophages have been applied successfully to control the growth of various foodborne pathogens. sequencing of their genomes is considered as an important preliminary step to ensure their safety prior to food applications. results: the lytic bacteriophage, phisbom-ag3, targets the important foodborne pathogen, shigella. it is morphologically similar to phage vii of salmonella enterica serovar typhi and a series of phages of acinetobacter calcoaceticus and rhizobium ... | 2011 | 21595934 |
| the population of a high-virulence strain of salmonella enterica serovar enteritidis in subcutaneously infected partridge: a quantitative time-course study using real-time pcr. | this research was undertaken to determine the population of a high-virulence strain of salmonella enterica serovar enteritidis in partridge by a fluorescent quencher pcr assay and to correlate these findings with the results obtained from the immunohistochemical localization and histopathological examinations of selected salmonella enterica serovar enteritidis-infected tissues. to make the results meaningful, a side-by-side bacteriology method (indirect immuno-fluorescent antibody staining) was ... | 2011 | 21594642 |
| oral and nasal dna vaccines delivered by attenuated salmonella typhimurium induce protective immune response against infectious bronchitis in chickens. | several studies have reported that intramuscular injection of dna vaccines against infectious bronchitis virus (ibv) induces protective immune responses. in the present study, we developed oral and nasal dna vaccines that carried the s1 gene and n gene of ibv delivered by attenuated salmonella typhimurium strains, sl/pv-s1 and sl/pv-n, respectively. the safety and stability of recombinant salmonella vaccine were evaluated. following oral and nasal administration of chickens, the serum and mucosa ... | 2011 | 21593235 |
| a holin remnant protein encoded by sty1365 is involved in envelope stability of salmonella enterica serovar typhi. | we characterized sty1365, a small orf of salmonella enterica serovar typhi. this 174-bp orf encodes a putative product of 57 amino acid residues with a premature stop codon. nevertheless, bioinformatic analyses revealed that the predicted product of sty1365 has similarity to putative holin genes of escherichia coli and bacteriophage φp27. sty1365 showed a high-level expression at the early log phase and a small corresponding protein product was detected mainly in the inner membrane fraction. clo ... | 2011 | 21592194 |
| helicobacter pylori exhibits a fur-dependent acid tolerance response. | background: helicobacter pylori colonizes the acid environment of the gastric mucosa. like other enteric bacterial pathogens, including salmonella enterica, which must survive a brief exposure to that environment, h. pylori displays a rapid response to subtle changes in ph, which confers an increased ability to survive at more extreme acidic ph. this two-step acid tolerance response (atr) requires de novo protein synthesis and is dependent on the function of the global regulatory protein fur. o ... | 2011 | 21585604 |
| selectivity of inhibition of n-succinyl-l,l-diaminopimelic acid desuccinylase in bacteria: the product of dape-gene is not the target of l-captopril antimicrobial activity. | the emergence of bacterial strains that are resistant to virtually all currently available antibiotics underscores the importance of developing new antimicrobial compounds. n-succinyl-l,l-diaminopimelic acid desuccinylase (dape) is a metallohydrolase involved in the meso-diaminopimelate (mdap)/lysine biosynthetic pathway necessary for lysine biosynthesis and for building the peptidoglycan cell wall. because dape is essential for gram-negative and some gram-positive bacteria, dape has been propos ... | 2011 | 21577314 |
| survival of salmonella strains differing in their biofilm-formation capability upon exposure to hydrochloric and acetic acid and to high salt. | acidic and osmotic treatments are part of hurdle systems to control pathogens such as salmonella in food. in the current study, salmonella enterica isolates previously shown to differ in their ability to form biofilms were grown in diluted tryptic soy broth (tsb) (1:5 dilution in distilled water) and subsequently exposed to phosphate-buffered saline (pbs) adjusted to ph 3.0 with hcl, pbs adjusted to ph 3.9 with acetic acid, or rice vinegar diluted 1:15 with distilled water (ph 3.9). cells grown ... | 2011 | 21576891 |
| early, myd88-dependent induction of interleukin-17a expression during salmonella colitis. | the development of t helper 17 (t(h)17) cells is a well-established adaptive mechanism for the production of interleukin (il)-17a, a cytokine involved in neutrophil recruitment. however, pathways contributing to mucosal expression of il-17a during the initial phase of a bacterial infection have received less attention. here we used the mouse colitis model of salmonella enterica serotype typhimurium (s. typhimurium) infection to investigate the contribution of myeloid differentiation primary resp ... | 2011 | 21576324 |
| fur activates the expression of salmonella enterica pathogenicity island 1 by directly interacting with the hild operator in vivo and in vitro. | previous studies have established that the expression of salmonella enterica pathogenicity island 1 (spi1), which is essential for epithelial invasion, is mainly regulated by the hild protein. the ferric uptake regulator, fur, in turn modulates the expression of the s. enterica hild gene, albeit through an unknown mechanism. here we report that s. enterica fur, in its metal-bound form, specifically binds to an at-rich region (boxa), located upstream of the hild promoter (p(hild)), at position -1 ... | 2011 | 21573071 |
| structural insight into the rotational switching mechanism of the bacterial flagellar motor. | the bacterial flagellar motor can rotate either clockwise (cw) or counterclockwise (ccw). three flagellar proteins, flig, flim, and flin, are required for rapid switching between the cw and ccw directions. switching is achieved by a conformational change in flig induced by the binding of a chemotaxis signaling protein, phospho-chey, to flim and flin. flig consists of three domains, flig(n), flig(m), and flig(c), and forms a ring on the cytoplasmic face of the ms ring of the flagellar basal body. ... | 2011 | 21572987 |
| population dynamics of salmonella enterica serotypes in commercial egg and poultry production. | fresh and processed poultry have been frequently implicated in cases of human salmonellosis. furthermore, increased consumption of meat and poultry has increased the potential for exposure to salmonella. while advances have been made in reducing the prevalence and frequency of salmonella contamination in processed poultry, there is mounting pressure on commercial growers to prevent and/or eliminate these human pathogens in preharvest production facilities. several factors contribute to salmonell ... | 2011 | 21571882 |
| subtyping salmonella serovar enteritidis isolates from different sources using sequence typing based on virulence genes and crisprs. | salmonella enterica subsp. enterica serovar enteritidis is a major cause of foodborne salmonellosis in the united states. two major food vehicles for ser. enteritidis are contaminated eggs and chicken meat. improved subtyping methods are needed to accurately track specific strains of ser. enteritidis related to human salmonellosis throughout the chicken and egg food system. a sequence typing scheme based on virulence genes (fimh and ssel) and crisprs (clustered regularly interspaced short palind ... | 2011 | 21571881 |
| inhibition studies with anions and small molecules of two novel β-carbonic anhydrases from the bacterial pathogen salmonella enterica serovar typhimurium. | two new β-carbonic anhydrases (cas, ec 4.2.1.1) from the bacterial pathogen salmonella enterica serovar typhimurium, stca 1 and stca 2, were characterized kinetically. the two enzymes possess appreciable activity as catalysts for the hydration of co(2) to bicarbonate, with k(cat) of 0.79×10(6)s(-1) and 1.0×10(6)s(-1), and k(cat)/k(m) of 5.2×10(7)m(-1)s(-1) and of 8.3×10(7)m(-1)s(-1), respectively. a large number of simple/complex inorganic anions as well as other small molecules (sulfamide, sulf ... | 2011 | 21570835 |
| lps structure influences protein secretion in salmonella enterica. | in this study we have compared protein secretion in the wild type of s. typhimurium and the rfac mutant. we found out that the rfac mutant was defective in protein secretion. in addition, the rfac mutant was defective in its invasion into an ipec-j2 porcine epithelial cell line and also in motility in semisolid agar. consistent with this, reduced flagella numbers were observed in the rfac mutant. in the rfac mutant, there were no defects in flagellin expression as detected by western blot and im ... | 2011 | 21570779 |
| application of multiple locus variable number of tandem repeat analysis (mlva), phage typing and antimicrobial susceptibility testing to subtype salmonella enterica serovar typhimurium isolated from pig farms, pork slaughterhouses and meat producing plants in ireland. | salmonella enterica subsp. enterica serovar typhimurium is a common zoonotic pathogen encountered in irish pigs and the pork industry and its characterisation using highly discriminatory typing methods is necessary for epidemiological studies, outbreak investigation and control. multiple locus variable number of tandem repeat analysis (mlva), phage typing and antimicrobial susceptibility testing were applied to characterise 301 s. typhimurium isolates of porcine origin isolated from farms, slaug ... | 2011 | 21569956 |
| distribution of salmonella typhimurium in romaine lettuce leaves. | leafy greens are occasionally involved in outbreaks of enteric pathogens. in order to control the plant contamination it is necessary to understand the factors that influence enteric pathogen-plant interactions. attachment of salmonella enterica serovar typhimurium to lettuce leaves has been demonstrated before; however, only limited information is available regarding the localization and distribution of immigrant salmonella on the leaf surface. to extend our knowledge regarding initial pathogen ... | 2011 | 21569943 |
| association of attenuated mutants of salmonella enterica serovar enteritidis with porcine peripheral blood leukocytes. | in this study, we were interested in the association of attenuated mutants of salmonella enterica serovar enteritidis with subpopulations of porcine white blood cells (wbc). the mutants included those with inactivated aroa, phop, rfal, rfag, rfac and flic genes and a mutant with five major pathogenicity islands removed (δspi1-5 mutant). using flow cytometry, we did not observe any difference in the interactions of the wild-type s. enteritidis, aroa and phop mutants with wbc. δspi1-5 and flic mut ... | 2011 | 21569080 |
| onset of diarrhea and pyrexia and time to detection of salmonella enterica subsp enterica in feces in experimental studies of cattle, horses, goats, and sheep after infection per os. | to determine time to first detection of salmonella organisms in feces of animals after experimental infection po and times to onset of diarrhea and pyrexia to evaluate a common method for identifying nosocomial infections on the basis of time of admission and onset of clinical signs (ie, the 3-day criterion). | 2011 | 21568780 |
| reemergence of chloramphenicol in typhoid fever in the era of antibiotic resistance. | 2010 | 21568009 | |
| two efficient methods for the conjugation of smooth-form lipopolysaccharides with probes bearing hydrazine or amino groups. ii. lps activation with a cyanopyridinium agent. | this chapter presents a conjugation method for coupling probes bearing hydrazine or primary amino groups to a lipopolysaccharide (lps). lps is modified by the activation of the hydroxyl groups present in its o-antigen moiety with 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (cdap). the method yields conjugates with good labeling ratios, preserving the endotoxic activity of the lipid a moiety. conjugation of smooth-form lps from salmonella enterica sv. minnesota with dansyl hydrazine and h ... | 2011 | 21567326 |
| two efficient methods for the conjugation of smooth-form lipopolysaccharides with probes bearing hydrazine or amino groups. i. lps activation with cyanogen bromide. | this chapter presents a conjugation method for coupling probes bearing hydrazine or primary amino groups to a smooth(s)-form lipopolysaccharide (lps). lps is modified by the activation of the hydroxyl groups present in its o-antigen moiety with cyanogen bromide in aqueous acetone. the method yields conjugates with good labeling ratios, preserving the endotoxic activity of the lipid a moiety. conjugation of smooth-form lps from salmonella enterica sv. minnesota with dansyl hydrazine and horseradi ... | 2011 | 21567325 |
| dry-resistance of salmonella enterica subsp. enterica serovar enteritidis is regulated by both sep22, a novel pathogenicity-related factor of salmonella, and nutrients. | environmental isolates of salmonella enterica serover enteritidis (s. enteritidis) clones were grown to the logarithmic phase, washed and re-suspended in saline or luria-bertani (lb) medium, and then 10-µl aliquots of the suspensions were dried overnight at room temperature. the dried bacteria were mixed with 1 ml of ice-cold pbs, suspended and examined for colony-forming activity. all of the pathogenic clones with high levels of sep22, identical to salmonella dps, maintained good viability if s ... | 2009 | 21566364 |
| nitrous oxide emission and microbial community in the rhizosphere of nodulated soybeans during the late growth period. | we examined n(2)o emissions from the rhizosphere of field-grown soybeans during the late growth stage (99-117 days after sowing). marked emissions were detected from the nodulated root systems of field-grown soybeans, whereas a non-nodulating soybean mutant showed no emission. degraded nodules exclusively generated the n(2)o. a culture-independent analysis of microbial communities showed bradyrhizobium sp., acidvorax facilis, salmonella enterica, xanthomonas sp., enterobacter cloacae, pseudomona ... | 2009 | 21566356 |
| x-ray crystallography and isothermal titration calorimetry studies of the salmonella zinc transporter zntb. | the zntb zn(2+) efflux system is important for maintenance of zn(2+) homeostasis in enterobacteria. we report crystal structures of zntb cytoplasmic domains from salmonella enterica serovar typhimurium (stzntb) in dimeric and physiologically relevant homopentameric forms at 2.3 å and 3.1 å resolutions, respectively. the funnel-like structure is similar to that of the homologous thermotoga maritima cora mg(2+) channel and a vibrio parahaemolyticus zntb (vpzntb) soluble domain structure. however, ... | 2011 | 21565704 |
| ethidium bromide efflux by salmonella: modulation by metabolic energy, ph, ions and phenothiazines. | the main efflux pump of salmonella enterica serotype enteritidis, which obtains its energy for the extrusion of noxious agents from the proton-motive force, was studied with the aid of an ethidium bromide (etbr) semi-automated method under conditions that define the role of metabolic energy, ions and ph in the extrusion of the universal substrate etbr. the results obtained in this study indicate that in minimal medium containing sodium at ph 5 efflux of etbr is independent of glucose, whereas at ... | 2011 | 21565465 |
| quantitative proteomic analysis of salmonella enterica serovar typhimurium under phop/phoq activation conditions. | the phop/phoq two-component system plays a central regulatory role in the pathogenesis of salmonella enterica serovar typhimurium (s. typhimurium), and it can be activated by low mg(2+) concentrations and sublethal concentrations of cationic antimicrobial peptides (camp). therefore, these two phop/phoq activation signals are considered as in vivo environmental cues sensed by s. typhimurium for adaptation and survival. in this work, we conducted a silac (stable isotope labeling by amino acids in ... | 2011 | 21563813 |
| receptor-transporter interactions of canonical atp-binding cassette import systems in prokaryotes. | atp-binding cassette (abc) transport systems mediate the translocation of solutes across biological membranes at the expense of atp. they share a common modular architecture comprising two pore-forming transmembrane domains and two nucleotide binding domains. in prokaryotes, abc transporters are involved in the uptake of a large variety of chemicals, including nutrients, osmoprotectants and signal molecules. in pathogenic bacteria, some abc importers are virulence factors. canonical abc import s ... | 2011 | 21561685 |
| virulotyping of salmonella enterica serovar napoli strains isolated in italy from human and nonhuman sources. | abstract salmonella enterica serovar napoli is an emerging serovar in italy, france, and switzerland, but little is known about its pathogenicity to humans. a collection of 112 strains of salmonella napoli isolated in italy from human cases, foods of animal origin, and the environment have been characterized by the detection of a set of virulence genes, pulsed-field gel electrophoresis (pfge), and antibiotic susceptibility. all the strains examined were susceptible to all the antimicrobials test ... | 2011 | 21561382 |
| food-specific attribution of selected gastrointestinal illnesses: estimates from a canadian expert elicitation survey. | abstract the study used a structured expert elicitation survey to derive estimates of food-specific attribution for nine illnesses caused by enteric pathogens in canada. it was based on a similar survey conducted in the united states and focused on campylobacter spp., escherichia coli o157:h7, listeria monocytogenes, nontyphoidal salmonella enterica, shigella spp., vibrio spp., yersinia enterocolitica, cryptosporidium parvum, and norwalk-like virus. a snowball approach was used to identify food ... | 2011 | 21561379 |
| crystallographic and microcalorimetric analyses reveal the structural basis for high arginine specificity in the salmonella enterica serovar typhimurium periplasmic binding protein stm4351. | 2011 | 21560168 | |
| paneth cell α-defensins in enteric innate immunity. | paneth cells at the base of small intestinal crypts of lieberkühn secrete high levels of α-defensins in response to cholinergic and microbial stimuli. paneth cell α-defensins are broad spectrum microbicides that function in the extracellular environment of the intestinal lumen, and they are responsible for the majority of secreted bactericidal peptide activity. paneth cell α-defensins confer immunity to oral infection by salmonella enterica serovar typhimurium, and they are major determinants of ... | 2011 | 21560070 |
| m153r mutation in a ph-sensitive green fluorescent protein stabilizes its fusion proteins. | green fluorescent protein (gfp) and its fusion proteins have been used extensively to monitor and analyze a wide range of biological processes. however, proteolytic cleavage often removes gfp from its fusion proteins, not only causing a poor signal-to-noise ratio of the fluorescent images but also leading to wrong interpretations. | 2011 | 21559297 |
| computational investigation of the enzymatic mechanisms of phosphothreonine lyase. | spvc, a virulence effector injected through type iii secretion system by some salmonella serovars, belongs to the newly discovered enzyme family, phosphothreonine lyase. previous experimental studies have demonstrated that spvc irreversibly inactivates mitogen-activated protein kinases by removing the phosphate group from phosphothreonine-containing substrate through a β-elimination mechanism, and results in a β-methyldehydroalanine product. interestingly, further biochemical investigations also ... | 2011 | 21558045 |
| adhesive mechanisms of salmonella enterica. | salmonella enterica is an invasive, facultative intracellular pathogen of animal and man with the ability to colonize various niches in diverse host organisms. the pathogenesis of infections by s. enterica requires adhesion to various host cell surfaces, and a large number of adhesive structures can be found. depending on the serotype of s. enterica, gene clusters for more than 10 different fimbrial adhesins were identified, with type i fimbriae such as fim, lpf (long polar fimbriae), tafi (thin ... | 2011 | 21557055 |
| protective role of akt2 in salmonella enterica serovar typhimurium-induced gastroenterocolitis. | the salmonella effector protein sopb has previously been shown to induce activation of akt and protect epithelial cells from apoptosis in vitro. to characterize the role of akt2 in host defense against salmonella typhimurium infection, wild-type (wt) mice and mice lacking akt2 (akt2 ko) were infected using a salmonella acute gastroenteritis model. infected akt2 ko mice showed a more pronounced morbidity and mortality associated with higher bacterial loads in the intestines, and elevated levels o ... | 2011 | 21555401 |
| immune response of chicken gut to natural colonisation by gut microflora and to salmonella enterica serovar enteritidis infection. | in commercial poultry production, there is a lack of natural flora providers since chickens are hatched in the clean environment of a hatchery. events occurring soon after hatching are therefore of particular importance and that is why we were interested in the development of the gut microbial community, the immune response to natural microbial colonisation and the response to salmonella enteritidis infection as a function of chicken age. the complexity of chicken gut microbiota gradually increa ... | 2011 | 21555397 |
| yqic of salmonella enterica serovar typhimurium is a membrane fusogenic protein required for mice colonization. | abstract: | 2011 | 21554724 |
| secondary renal tubular acidosis in a hereford calf. | a 3-month-old hereford heifer calf was presented for lethargy. blood gas analysis and plasma biochemical testing revealed severe metabolic acidosis, azotemia, hyponatremia, hyperchloremia, and normal anion gap. results of a urinalysis were consistent with acute tubular necrosis with inadequate acidification of urine based on the degree of acidemia. salmonella enterica serovar agona was cultured from both urine and feces. the calf was treated with intravenous polyionic fluids, bicarbonate, and an ... | 2011 | 21554363 |
| n-benzyl-3-sulfonamidopyrrolidines are a new class of bacterial dna gyrase inhibitors. | this paper characterizes n-benzyl-3-sulfonamidopyrrolidines (gyramides) as dna gyrase inhibitors. gyramide a was previously shown to exhibit antimicrobial activity that suggested it inhibited bacterial cell division. in this study, we conducted target identification studies and identified dna gyrase as the primary target of gyramide a. the gyramide a resistance-determining region in dna gyrase is adjacent to the dna cleavage gate and is a new site for inhibitor design. we studied the antibiotic ... | 2011 | 21552338 |
| crystal structures of slya, a master virulence regulator of salmonella, in free and dna-bound states. | slya is a master virulence regulator that controls the transcription of numerous genes in salmonella enterica. we present here crystal structures of slya by itself and bound to a high-affinity dna operator sequence in the slya gene. slya interacts with dna through direct recognition of a guanine base by r65, as well as interactions between conserved r86 and the minor groove and a large network of non-base-specific contacts to the sugar-phosphate backbone. our structures, together with an unpubli ... | 2011 | 21550983 |
| prevalence of salmonella enterica, listeria monocytogenes, and escherichia coli virulence factors in bulk tank milk and in-line filters from u.s. dairies. | the zoonotic bacteria salmonella enterica, listeria monocytogenes, and escherichia coli are known to infect dairy cows while not always causing clinical signs of disease. these pathogens are sometimes found in raw milk, and human disease outbreaks due to these organisms have been associated with the consumption of raw milk or raw milk products. bulk tank milk (btm) samples (536) and in-line milk filters (519) collected from dairy farms across the united states during the national animal health m ... | 2011 | 21549046 |
| efficacy of ε-polylysine, lauric arginate, or acidic calcium sulfate applied sequentially for salmonella reduction on membrane filters and chicken carcasses. | salmonella contamination continues to be one of the major concerns for the microbiological safety of raw poultry products. application of more than one decontamination agent as a multihurdle intervention to carcasses in a processing line might produce greater reductions than one treatment alone due to different modes of action of individual antimicrobials. in this study, all possible two-way combinations and individual applications of ε-polylysine (epl), lauric arginate (lae), and acidic calcium ... | 2011 | 21549044 |
| masscode liquid arrays as a tool for multiplexed high-throughput genetic profiling. | multiplexed detection assays that analyze a modest number of nucleic acid targets over large sample sets are emerging as the preferred testing approach in such applications as routine pathogen typing, outbreak monitoring, and diagnostics. however, very few dna testing platforms have proven to offer a solution for mid-plexed analysis that is high-throughput, sensitive, and with a low cost per test. in this work, an enhanced genotyping method based on masscode technology was devised and integrated ... | 2011 | 21544191 |
| systemic and mucosal immunity induced by attenuated salmonella enterica serovar typhimurium expressing orf7 of porcine reproductive and respiratory syndrome virus. | oral administration of attenuated salmonella vaccine may provide valuable advantages such as low cost, easy preparation, and safety. attenuated salmonella vaccines also serve as carriers of foreign antigens and immunomodulatory cytokines. presently, an attenuated salmonella enterica serovar typhimurium strain was used as a carrier for open reading frame 7 (orf7) protein of porcine reproductive and respiratory syndrome virus (prrsv), a swine pathogen of significant global economic importance. ini ... | 2011 | 21543119 |
| active suppression of early immune response in tobacco by the human pathogen salmonella typhimurium. | the persistence of enteric pathogens on plants has been studied extensively, mainly due to the potential hazard of human pathogens such as salmonella enterica being able to invade and survive in/on plants. factors involved in the interactions between enteric bacteria and plants have been identified and consequently it was hypothesized that plants may be vectors or alternative hosts for enteric pathogens. to survive, endophytic bacteria have to escape the plant immune systems, which function at d ... | 2011 | 21541320 |
| a comprehensive study of the contribution of salmonella enterica serovar typhimurium spi2 effectors to bacterial colonization, survival, and replication in typhoid fever, macrophage, and epithelial cell infection models. | salmonella enterica serovars are gram-negative bacterial pathogens responsible for human diseases including gastroenteritis and typhoid fever. after ingestion, salmonella cross the intestinal epithelial barrier, where they are phagocytosed by macrophages and dendritic cells, which then enables their spread to systemic sites during cases of typhoid fever. salmonella use two type 3 secretion systems encoded by salmonella pathogenicity islands (spi) 1 and 2 to inject virulence proteins into host ce ... | 2011 | 21540636 |
| [experiences in the epidemiological surveillance of foodborne pathogens by pulsed field gel electrophoresis (pfge) in peru.] | foodborne diseases and other enteric infections often occur as outbreaks and cause morbidity and mortality all over the world. in perú, they represent a serious public health problem, and are caused by a great variety of infectious agents. for epidemiological research, a wide array of typification methods are used. one of the most important tools for the molecular subtyping of bacterial pathogens is the pulsed field gel electrophoresis (pfge), which is a highly precise method that allows the dis ... | 2011 | 21537781 |
| the antibiotic dehydrophos is converted to a toxic pyruvate analog by peptide bond cleavage in salmonella enterica. | the metabolic processing of dehydrophos, a broad-spectrum peptide antibiotic containing an unusual vinyl-phosphonate moiety, was examined using a panel of salmonella enterica mutants deficient in peptide uptake and catabolism. dehydrophos bioactivity is lost in opp, tpp double mutants, demonstrating a requirement for uptake via non-specific oligopeptide permeases. dehydrophos bioactivity is also abolished in a quadruple salmonella mutant lacking the genes encoding peptidases a, b, d, and n, show ... | 2011 | 21537024 |
| chronic typhoid infection and the risk of biliary tract cancer and stones in shanghai, china. | abstract: previous studies have shown a positive association between chronic typhoid carriage and biliary cancers. we compared serum salmonella enterica serovar typhi antibody titers between biliary tract cancer cases, biliary stone cases without evidence of cancer, and healthy subjects in a large population-based case-control study in shanghai, china.participants included 627 newly diagnosed primary biliary tract cancer patients; 1,037 biliary stone cases (774 gallbladder and 263 bile-duct) and ... | 2011 | 21535882 |
| application of polylactic acid coating with antimicrobials in reduction of escherichia coli o157:h7 and salmonella stanley on apples. | survival of escherichia coli o157:h7 and salmonella stanley on apples as affected by application of polylactic acid (pla) coating with antimicrobials was investigated. golden delicious apples were spot inoculated with e. coli o157:h7 or s. stanley and spray coated with pla solutions containing lactic acid (la), disodium ethylenediaminetetraacetic acid (edta), sodium benzoate (sb), potassium sorbate (ps), or their combination (la + edta, sb + la, sb + la + edta). apples without any coating treatm ... | 2011 | 21535842 |
| modification of buffered peptone water for improved recovery of heat-injured salmonella typhimurium. | rapid detection of salmonella in foods is often limited by the high demand for the sensitivity of detection, poor physiological conditions of the target cells, and high concentration of background flora. in this study, the conditions of nonselective enrichment cultivation were modified in order to improve the quantitative detection of heat-injured salmonella in minced meat. the effect of the modifications on the recovery was observed by means of rna-based sandwich hybridization, which was adjust ... | 2011 | 21535838 |