Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| effect of temperature and commodity on insecticidal efficacy of spinosad dust against sitophilus oryzae (coleoptera: curculionidae) and rhyzopertha dominica (coleoptera: bostrychidae). | the insecticidal effect of spinosad dust, a formulation that contains 0.125% spinosad, was evaluated against adults of sitophilus oryzae (l.) and rhyzopertha dominica (f.) at three temperature levels (20, 25, and 30 degrees c) and four commodities (wheat, triticum aestivum l.; barley, hordeum vulgare l.; rice, oryza sativa l.; and maize, zea mays l.). for this purpose, quantities of the above-mentioned grains were treated with spinosad at two dose rates (20 and 50 ppm of the formulation, corresp ... | 2008 | 18613602 |
| acc homoeoloci and the evolution of wheat genomes. | the dna sequences of wheat acc-1 and acc-2 loci, encoding the plastid and cytosolic forms of the enzyme acetyl-coa carboxylase, were analyzed with a view to understanding the evolution of these genes and the origin of the three genomes in modern hexaploid wheat. acc-1 and acc-2 loci from each of the wheats triticum urartu (a genome), aegilops tauschii (d genome), triticum turgidum (ab genome), and triticum aestivum (abd genome), as well as two acc-2-related pseudogenes from t. urartu were sequen ... | 2008 | 18599450 |
| equilibrium and kinetics of biosorption of cadmium(ii) and copper(ii) ions by wheat straw. | biosorption equilibrium and kinetics of cd(2+) and cu(2+) ions on wheat straw, triticum aestivum, in an aqueous system were investigated. among the models tested, namely the langmuir, freundlich, temkin, and dubinin-radushkevich isotherms, the biosorption equilibrium for both cd(2+) and cu(2+) was best described by the langmuir model. the langmuir biosorption capacity for cd(2+) was about 27% higher than that for cu(2+). it was also found that biosorption of cd(2+) and cu(2+) by wheat straw foll ... | 2009 | 18599289 |
| effects of elevated atmospheric co2 concentrations on the quantitative protein composition of wheat grain. | the continuing increase in atmospheric co 2 concentration is predicted to enhance biomass production and to alter biochemical composition of plant tissues. in the present study, winter wheat ( triticum aestivum l. cv. 'batis') was grown under ambient air (blow, co 2 concentration: 385 mul l (-1)) and free-air co 2 enrichment (face, co 2 concentration: 550 mul l (-1)) and two different nitrogen (n) fertilization levels (normal n supply: n100, 50% of normal n supply: n50). mature kernels were mill ... | 2008 | 18598044 |
| analysis of introgression of aegilops ventricosa tausch. genetic material in a common wheat background using c-banding. | seven triticum aestivum (cv. moisson)-aegilops ventricosa addition lines and four vpm-1 lines were studied by c-banding, and compared with the parental common wheat cultivars marne-desprez (hereafter marne), moisson, and a. ventricosa lines 10 and 11. all of the vpm-1 lines had similar c-banding patterns and carried the same major 5b:7b translocation as the parental marne cultivar. according to the c-banding analysis, the vpm-1 lines carry a complete 7d(7d(v)) chromosome substitution and a trans ... | 2008 | 18597067 |
| effects of as on as uptake, speciation, and nutrient uptake by winter wheat (triticum aestivum l.) under hydroponic conditions. | a hydroponic experiment was conducted to investigate the effects of arsenic (as) stress on growth, nutrition and as uptake, and speciation in shoots and roots of winter wheat (triticum aestivum l.). winter wheat has high tolerance to as. most as is accumulated in the roots, and an as concentration of 4,421 mg/kg was observed at a solution concentration of 20 mg/l as. arsenic concentrations in roots were approximately 40-100 times greater than those in shoots. arsenic in winter wheat roots and sh ... | 2008 | 18595400 |
| phytochemical quantification and total antioxidant capacities of emmer (triticum dicoccon schrank) and einkorn (triticum monococcum l.) wheat landraces. | in this study, samples of 18 ancient wheat (12 emmer, 6 einkorn) and 2 bread wheat varieties grown in different regions of turkey were examined for their total phenolics and flavonoids, phenolic acids, lutein, total yellow pigment, and total radical scavenging capacities against abts cation. results showed that health beneficial phytochemicals and total antioxidant capacities were generally significantly different in emmer and einkorn wheat groups. remarkably higher total antioxidant activity (1 ... | 2008 | 18593174 |
| a chalcone synthase-like gene is highly expressed in the tapetum of both wheat (triticum aestivum l.) and triticale (xtriticosecale wittmack). | a novel anther-specific chalcone synthase-like gene, tachsl1, was isolated and characterized. the tachsl1 transcript was detected only within the tapetum during the "free" and early vacuolated microspore stages in both wheat and triticale. sequence analysis indicated that the 41.8 kda tachsl1 deduced protein belongs to a small distinct group of type iii polyketide synthases that includes angiosperm and gymnosperm orthologs shown to be anther-specific. tachsl1 sequence characteristics and conserv ... | 2008 | 18592248 |
| quackgrass- and ryegrass-adapted populations of the cereal rust mite, abacarus hystrix (acari: eriophyidae), differ in their potential for wheat, triticum aestivum, colonization. | the cereal rust mite, abacarus hystrix, is one of the most notable among mites causing losses in cultivated grasslands. it is one of a few eriophyoid species for which a broad host range has been reported. recent studies, however, have shown that host specialization is very likely in this species. for two populations of a. hystrix (one inhabiting perennial ryegrass, the second inhabiting quackgrass), host-associated differences correlated with strong host fidelity, distinct phenotypes and reprod ... | 2009 | 18590598 |
| identification and mapping qtl for high-temperature adult-plant resistance to stripe rust in winter wheat (triticum aestivum l.) cultivar 'stephens'. | high-temperature adult-plant (htap) resistance from the winter wheat (triticum aestivum) cultivar 'stephens' has protected wheat crops from stripe rust caused by puccinia striiformis f. sp. tritici for 30 years. the objectives of this study were to identify quantitative trait loci (qtl) for htap resistance in stephens through genetic linkage analysis and identify dna markers linked to the qtl for use in marker-assisted breeding. mapping populations consisted of 101 recombinant inbred lines (rils ... | 2008 | 18584147 |
| scenario modeling potential eco-efficiency gains from a transition to organic agriculture: life cycle perspectives on canadian canola, corn, soy, and wheat production. | we used life cycle assessment to scenario model the potential reductions in cumulative energy demand (both fossil and renewable) and global warming, acidifying, and ozone-depleting emissions associated with a hypothetical national transition from conventional to organic production of four major field crops [canola (brassica rapa), corn (zea mays), soy (glycine max), and wheat (triticum aestivum)] in canada. models of these systems were constructed using a combination of census data, published va ... | 2008 | 18574623 |
| [characterization of triticum aestivum-dasypyrum villosum germplasms shannong05078]. | a germplasm line shannong05078 was developed from the progenies of am3 x triticum aestivum-dasypyrum villosum amphidiploid, the purpose of this study is to ascertain the genome composition of shannong05078 and then supply wheat breeding with reference. the results showed that shannong05078 was stable in morphology characters and presenting favorable agronomic traits, it was resistant to powdery mildew and rust. its protein content was higher, and the hwm-gs of it was 7+8, 5+10. the chromosome nu ... | 2008 | 18567504 |
| variation for homoeologous gene silencing in hexaploid wheat. | the absence of expression of individual members of a homoeologous set of genes in a polyploid is a well-established phenomenon. however, the extent to which such 'homoeologous silencing' can vary between individual genotypes within a species is unexplored. we have used the single-strand conformation polymorphism assay to identify homoeologue non-expression at 15 single-copy genes across a panel of 16 wheat varieties, representative of the genetic diversity present in modern northern european win ... | 2008 | 18564382 |
| the origin of a "zebra" chromosome in wheat suggests nonhomologous recombination as a novel mechanism for new chromosome evolution and step changes in chromosome number. | an alloplasmic wheat line, ta5536, with the "zebra" chromosome z5a was isolated from an elymus trachycaulus/triticum aestivum backcross derivative. this chromosome was named "zebra" because of its striped genomic in situ hybridization pattern. its origin was traced to nonhomologous chromosome 5a of wheat and 1h(t) of elymus; four chromatin segments were derived from chromosome 1h(t) and five chromatin segments including the centromere from 5a. in this study, our objective was to determine the me ... | 2008 | 18562667 |
| ferulic acid from aleurone determines the antioxidant potency of wheat grain (triticum aestivum l.). | grain is an important source of phytochemicals, which have potent antioxidant capacity. they have been implicated in the beneficial health effect of whole grains in reducing cardiovascular disease and type 2 diabetes. the aim of the present study was to identify the most important antioxidant fractions of wheat grain. it was found that the aleurone content of these fractions was highly correlated with the antioxidant capacity of the fractions (r = 0.96, p < 0.0001). ferulic acid appeared to be t ... | 2008 | 18558703 |
| effect of environmental stress during grain filling on the soluble proteome of wheat (triticum aestivum) dough liquor. | the influence of genotype and environment on a soluble wheat dough liquor proteome was studied for four cultivars grown under field conditions and under hot/dry and cool/wet regimes by two-dimensional electrophoresis followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry or quadrupole time-of-flight mass spectrometry. although the four cultivars had similar patterns, differences in the relative abundances of some components were observed. similarly, some differe ... | 2008 | 18553920 |
| novel properties of the wheat aluminum tolerance organic acid transporter (taalmt1) revealed by electrophysiological characterization in xenopus oocytes: functional and structural implications. | many plant species avoid the phytotoxic effects of aluminum (al) by exuding dicarboxylic and tricarboxylic acids that chelate and immobilize al(3+) at the root surface, thus preventing it from entering root cells. several novel genes that encode membrane transporters from the almt and mate families recently were cloned and implicated in mediating the organic acid transport underlying this al tolerance response. given our limited understanding of the functional properties of almts, in this study ... | 2008 | 18550686 |
| genetic mapping of adult plant leaf rust resistance genes lr48 and lr49 in common wheat. | hypersensitive adult plant resistance genes lr48 and lr49 were named based on their genetic independence of the known adult plant resistance genes. this study was planned to determine genomic locations of these genes. recombinant inbred line populations derived from crosses involving csp44 and vl404, sources of lr48 and lr49, respectively, and the susceptible parent wl711, were used to determine the genomic locations of these genes. bulked segregant analyses were performed using multiplex-ready ... | 2008 | 18542911 |
| quantitative trait loci for resistance to pre-harvest sprouting in us hard white winter wheat rio blanco. | pre-harvest sprouting (phs) of wheat is a major problem that severely limits the end-use quality of flour in many wheat-growing areas worldwide. to identify quantitative trait loci (qtls) for phs resistance, a population of 171 recombinant inbred lines (rils) was developed from the cross between phs-resistant white wheat cultivar rio blanco and phs-susceptible white wheat breeding line nw97s186. the population was evaluated for phs in three greenhouse experiments and one field experiment. after ... | 2008 | 18542909 |
| [cellular features of immune reaction of common wheat with lr19 gene to brown rust fungus infection]. | the effect of resistant gene lr19 on the development of brown rust fungus puccinia triticina in the immune line of thatcher variety was similar to that of nonhost species. the development of the fungus was inhibited in the plant tissue at the early stages. the fungus cells demonstrated structural disorganization of the nuclei and mitochondria before host cell invasion. the plant cell membranes and chloroplasts were changed; large chromatin aggregates appeared in their nuclei. hypersensitive reac ... | 2008 | 18540192 |
| quantitative trait loci for grain fructan concentration in wheat (triticum aestivum l.). | fructans (fructo-oligosaccharides) are prebiotics that are thought to selectively promote the growth of colonic bifidobacteria, thereby improving human gut health. fructans are present in the grain of wheat, a staple food crop. in the research reported here, we aimed to detect and map loci affecting grain fructan concentration in wheat using a doubled-haploid population derived from a cross between a high-fructan breeding line, berkut, and a low-fructan cultivar, krichauff. fructan concentration ... | 2008 | 18536901 |
| the effect of temperature shock and grain morphology on alpha-amylase in developing wheat grain. | the premature production of alpha-amylase without visible germination has been observed in developing grain of many cereals. the phenomenon is associated with cool temperatures in the late stages of grain growth but the mechanisms behind it are largely unknown. the aim of this study was to replicate the phenomenon under controlled conditions and investigate the possibility of a mechanistic link with grain size or endosperm cavity size. | 0 | 18535012 |
| wheat genomics: present status and future prospects. | wheat (triticum aestivum l.), with a large genome (16000 mb) and high proportion ( approximately 80%) of repetitive sequences, has been a difficult crop for genomics research. however, the availability of extensive cytogenetics stocks has been an asset, which facilitated significant progress in wheat genomic research in recent years. for instance, fairly dense molecular maps (both genetic and physical maps) and a large set of ests allowed genome-wide identification of gene-rich and gene-poor reg ... | 2008 | 18528518 |
| toxic reactivity of wheat (triticum aestivum) plants to herbicide isoproturon. | the herbicide isoproturon is widely used for controlling weed/grass in agricultural practice. however, the side effect of isoproturon as contaminants on crops is unknown. in this study, we investigated isoproturon-induced oxidative stress in wheat ( triticum aestivum). the plants were grown in soils with isoproturon at 0-20 mg/kg and showed negative biological responses. the growth of wheat seedlings with isoproturon was inhibited. chlorophyll content significantly decreased at the low concentra ... | 2008 | 18522406 |
| sir charles edward saunders, dominion cerealist. | charles edward saunders was born in london, ontario, in 1867. his father, sir william saunders, was the first director of the dominion experimental farms (1886-1911). charles received his b.a. with honours in science from the university of toronto in 1888 and his ph.d. in chemistry from johns hopkins university in baltimore, maryland, in 1891. he attempted a career in music, his first love, from 1893 to 1902. with his father, charles attended the 1902 international conference on plant breeding a ... | 2008 | 18521125 |
| data mining for mirnas and their targets in the triticeae. | micrornas (mirnas) and the mrna targets of mirnas were identified by sequence complementarity within a dna sequence database for species of the triticeae. data screening identified 28 mirna precursor sequences from 15 mirna families that contained conserved mature mirna sequences within predicted stem-loop structures. in addition, the identification of 337 target sequences among triticeae genes provided further evidence of the existence of 26 mirna families in the cereals. microrna targets inclu ... | 0 | 18521122 |
| mapping of a major locus controlling seed dormancy using backcrossed progenies in wheat (triticum aestivum l.). | seed dormancy is an important factor regulating preharvest sprouting (phs) but is a complex trait for genetic analysis. we previously identified a major quantitative trait locus (qtl) controlling seed dormancy on the long arm of chromosome 4a (4al) in common wheat. to transfer the qtl from the dormant lines 'os21-5' and 'leader' into the japanese elite variety 'haruyokoi', which has an insufficient level of seed dormancy, backcrossing was carried out through marker-assisted selection (mas) using ... | 2008 | 18521121 |
| variations of tandem repeat, regulatory element, and promoter regions revealed by wheat-rye amphiploids. | to better understand the evolution of allopolyploids, 4 different combinations between wheat (triticum aestivum l.) and rye (secale cereale l.) including 12 f1 hybrids and 12 derived amphiploids were analyzed and compared with their direct parental plants by pcr analysis using 150 wheat ssr (single sequence repeat) markers and by fish analysis using a rye-specific repetitive sequence (psc200) as a probe. nine ssr markers amplified rye-specific fragments whose sizes ranged from 471 bp to 1089 bp. ... | 2008 | 18521118 |
| the wheat pkaba1-interacting factor taabf1 mediates both abscisic acid-suppressed and abscisic acid-induced gene expression in bombarded aleurone cells. | to investigate the crosstalk of abscisic acid (aba) and gibberellin (ga) signaling in wheat (triticum aestivum), we have focused on the transcription factor taabf1. taabf1 (a member of the aba response element binding factor family) physically interacts with pkaba1, a signaling component in the aba-suppression of ga-induced gene expression in cereal grains. constitutive expression of taabf1 in aleurone cells of imbibing grains completely eliminated ga-induced expression from the amy32b promoter. ... | 2008 | 18516686 |
| molecular mapping of quantitative trait loci for yield and yield components in spring wheat (triticum aestivum l.). | an f1 derived doubled haploid (dh) population of 402 lines from the adapted spring wheat cross superb (high yielding)/bw278 (low yielding) was developed to identify quantitative trait loci (qtl) associated with yield and yield components. a subset of the population (186 lines) was evaluated in replicated field trials in 2001 and 2002 at six locations in manitoba and saskatchewan, canada. agronomic parameters, grain yield and yield components including 1,000 grain weight, harvest index, average s ... | 2008 | 18516583 |
| clomiphene citrate modulates the expression of endometrial carbohydrates (especially n-acetyl-d-glucosamine and sialic acid) in pseudopregnant rats. | this study investigated the presence of carbohydrates n-acetyl-d-glucosamine and sialic acid oligosaccharides, in the uterine epithelium of pseudopregnant rats treated with ovarian hormones and clomiphene citrate (cc) a commonly used fertility drug associated with low pregnancy rates. ovariectomized sexually mature rats were given 0.25mg cc prior to the implantation-priming hormone sequence of 5mg progesterone for 3 days and a single dose of 0.5microg estradiol-17beta (e(2)) on day 3 (pp(pe)) an ... | 2008 | 18514805 |
| uptake of zn and fe by wheat (triticum aestivum var. greina) and transfer to the grains in the presence of chelating agents (ethylenediaminedisuccinic acid and ethylenediaminetetraacetic acid). | a way to decrease iron and zinc deficiency in humans is to biofortify foods by increasing the bioavailable contents in these elements. the aim of this work was to study if chelating agents could be used to increase the capture of fe and zn by wheat grains. zn and/or fe in combination with the chelating agents ethylenediaminedisuccinic acid (edds) or ethylenediaminetetraacetic acid (edta) were added at various times (i.e., at flower head formation, anthesis, and postanthesis) to spring wheat ( tr ... | 2008 | 18512939 |
| quantitative expression analysis of selected cor genes reveals their differential expression in leaf and crown tissues of wheat (triticum aestivum l.) during an extended low temperature acclimation regimen. | a number of cor genes (cold-regulated genes) have been implicated in the acquisition of low temperature (lt) tolerance in wheat (triticum aestivum l.). this study compared the relative expression patterns of selected cor genes in leaf and crown tissues of wheat near-isogenic lines to increase understanding of the molecular mechanisms underlying lt acclimation. reciprocal near-isogenic lines were generated such that the dominant vrn-a1 and recessive vrn-a1 loci were interchanged in a spring cv. m ... | 2008 | 18508811 |
| polyphenol oxidase from wheat bran is a serpin. | polyphenol oxidase (ppo; ec 1.10.3.2) was isolated from wheat bran by a procedure that included ammonium sulfate fractionation, batch adsorption by deae-cellulofine, cm-cellulofine column chromatography, deae-cellulofine column chromatography, preparative isoelectric focusing, adsorption on the membrane of a vivapure q maxi h spin column, and heat treatment. these procedures led to 150-fold purification with 4.2% recovery. the ppo was homogeneous by sds/page. the relative molecular weight of the ... | 2008 | 18506224 |
| study of the refirming effect of a plant complex. | loss of skin elasticity is one of the main problems of ageing. this is a mechanical property influenced by elastin, a protein in the dermis which, together with collagen and glycosaminoglycans, makes up the connective tissue. this tissue is affected by a large number of events (such as cutaneous ageing, pregnancy, slimming processes and cellulitis) which eventually cause it to change. at the same time, the metabolism of the proteins of the connective tissue decreases and there is an ever greater ... | 1998 | 18505506 |
| identification and mapping of pm2026: a recessive powdery mildew resistance gene in an einkorn (triticum monococcum l.) accession. | triticum monococcum accession ta2026 showed resistance to wheat powdery mildew. to identify the resistance gene and transfer it to common wheat, genetic analysis and molecular mapping were conducted using an f2 population and derived f3 families from the cross of ta2026xm389. the results indicated that ta2026 possessed a recessive powdery mildew resistance gene. this gene was mapped to the terminal portion of chromosome 5aml and flanked by ssr marker loci xcfd39 and xgwm126. eight rflp markers p ... | 2008 | 18504540 |
| physical organisation of simple sequence repeats (ssrs) in triticeae: structural, functional and evolutionary implications. | a significant fraction of the nuclear dna of all eukaryotes is occupied by simple sequence repeats (ssrs) or microsatellites. this type of sequence has sparked great interest as a means of studying genetic variation, linkage mapping, gene tagging and evolution. although ssrs at different positions in a gene help determine the regulation of expression and the function of the protein produced, little attention has been paid to the chromosomal organisation and distribution of these sequences, even ... | 2008 | 18504349 |
| detection of quantitative trait loci for heading date based on the doubled haploid progeny of two elite chinese wheat cultivars. | quantitative trait loci (qtls) with epistatic and qtl x environment (qe) interaction for heading date were studied using a doubled haploid (dh) population containing 168 progeny lines derived from a cross between two elite chinese wheat cultivars huapei 3 x yumai 57 (triticum aestivum l.). a genetic map was constructed based on 305 marker loci, consisting of 283 ssr loci and 22 est-ssr markers, which covered a total length of 2141.7 cm with an average distance of 7.02 cm between adjacent markers ... | 2009 | 18500653 |
| construction of multiform scfv antibodies using linker peptide. | multiform single chain variable fragments (scfvs) including different length linker scfvs and bispecific scfv were constructed. the linker lengths of 0, 3, 5, 8, 12, and 15 amino acids between v(h) and v(l) of antideoxynivalenol (anti-don) scfv were used to analyze the affinities of scfvs. the affinity constants of these scfvs increased when the linker was lower than 12 amino acids. the affinity constant would not change when the linker was longer than 12 amino acids. fusion gene of anti-don scf ... | 2008 | 18499076 |
| [the effect of biologically active substances from coniferous plants on the l-phenylalanine ammonia lyase and peroxidase activities in wheat leaves]. | the effect of the preparations produced from needles and wood of various coniferous species on the activities of l-phenylalanine ammonia lyase (pal; ec 4.3.1.5) and peroxidase (po; ec 1.11.1.7), the enzymes involved in the development of plant defense response, was studied. it was demonstrated that treatment of soft wheat (triticum aestivum l.) primary leaves with biological preparations produced from coniferous plants caused a transient increase in pal and po activities. the induction of these ... | 2009 | 18491609 |
| [inhibition of lipase activity by low-molecular-weight chitosan]. | inhibition of enzymatic activity of lipase (ec 3.1.1.3) from the fungus candida rugosa and wheat (triticum aestivum l.) germ by low-molecular-weight chitosan with an average molecular weight of 5.7 kda in reactions of p-nitrophenyl palmitate cleavage was studied. preincubation of lipases with chitosan, prior to addition of the substrate to solution, showed that equilibrium during the lipase-inhibitor complex formation was reached within 30 min. the inhibition constants for c. rugosa lipase and w ... | 2008 | 18491595 |
| high level of conservation between genes coding for the gamyb transcription factor in barley (hordeum vulgare l.) and bread wheat (triticum aestivum l.) collections. | the transcription factor gamyb is involved in gibberellin signalling in cereal aleurone cells and in plant developmental processes. nucleotide diversity of hvgamyb and tagamyb was investigated in 155 barley (hordeum vulgare) and 42 wheat (triticum aestivum) accessions, respectively. polymorphisms defined 18 haplotypes in the barley collection and 1, 7 and 3 haplotypes for the a, b, and d genomes of wheat, respectively. we found that (1) hv- and tagamyb genes have identical structures. (2) both g ... | 2008 | 18488187 |
| [isolation and characterization of a low molecular weight glutenin gene from taenitherum nevski]. | more and more low-molecular-weight glutenin(lmw glutenin) genes were isolated and characterized from hexaploid wheat (triticum aestivum l.). however, few homologous genes were obtained from its relative species, which limited our understanding of the relationships among them. therefore, it is necessary to isolate lmw glutenin homologous genes from wheat wild relative species. using a pair of specific oligonucleotide pcr primers for taenitherum genomic dna, a lmw glutenin gene sequence, with nucl ... | 2008 | 18487155 |
| lateral-flow colloidal gold-based immunoassay for the rapid detection of deoxynivalenol with two indicator ranges. | a lateral-flow immunoassay using a colloidal gold-labelled monoclonal antibody was developed for the rapid detection of deoxynivalenol (don). different parameters, such as the amount of immunoreagents, type of the materials, composition of the blocking solution and of the detector reagent mixture, were investigated to provide the optimum assay performance. the experimental results demonstrated that such a visual test had an indicator range rather than a cut-off value. thus, tests for don determi ... | 2008 | 18482609 |
| [comparison of eddy covariance and static chamber/gas chromatogram methods in measuring ecosystem respiration]. | based on the measurement of carbon flux by the methods of eddy covariance and static chamber/gas chromatogram, a comparison was made between the two methods in evaluating ecosystem respiration over winter wheat (triticum aestivum)--summer maize (zea mays) double cropland and kobresia humilis alpine meadow. the results showed that under the conditions of obtained data having good quality, nighttime ecosystem respiration from eddy covariance measurement was significantly agreed with that from stat ... | 2008 | 18472446 |
| the expression of aluminum stress induced polypeptides in a population segregating for aluminum tolerance in wheat (triticum aestivum l.). | this study examined the changes in gene expression induced by aluminum (al) stress in wheat root tips. seedlings of triticum aestivum l. cvs. katepwa (al sensitive), maringa (al tolerant), and alikat (al tolerant near isoline; 'katepwa'*3/'maringa') and a f2 population derived from 'katepwa' x 'alikat', were grown for 3 days in either 0 or 1 μg∙ml−1 al. polypeptides were labeled with 35s-methionine prior to separation by gel electrophoresis. there were a few polypeptides from who ... | 1995 | 18470241 |
| differentiation between wheat chromosomes 4b and 4d. | a linkage map based on homoeologous recombination, induced by the absence of the ph1 locus, between chromosome 4d of triticum aestivum l. (genomes aabbdd) and chromosome 4b of t. turgidum l. (genomes aabb) was compared with a linkage map of chromosome 4am of t. monococcum l. and a consensus map of chromosomes 4b and 4d of t. aestivum based on homologous recombination. the 4d/4b homoeologous map was only one-third the length of the homologous maps and all intervals were reduced relative to the 4b ... | 1995 | 18470237 |
| expression of the cold-induced wheat gene wcs120 and its homologs in related species and interspecific combinations. | low-temperature response was measured at the whole plant and at the molecular level in wheat-rye amphiploids and in other interspecific combinations. cold tolerance of interspecifics whose parents diverged widely in hardiness levels resembled the less hardy higher ploidy level wheat parent. expression of the low-temperature induced wcs120 gene of wheat (triticum aestivum l. em. thell.) has been associated with freezing tolerance and was used here to study mrna and protein accumulation in intersp ... | 1995 | 18470225 |
| nonrandom chromosome variation and morphogenic potential in cell lines of bread wheat (triticum aestivum l.). | a cytogenetical analysis of 18 cell lines, 9 microspore derived, 6 anther derived, and 3 immature-embryo derived, of bread wheat (triticum aestivum l.) varying in their morphogenic potential was undertaken. chromosome variation, both structural and numerical, was detected in all lines studied. variation was present and, in some cases quite extensive, in the earliest samples taken (only 12 weeks after initiation of the suspensions). within any culture, the pattern and extent of variation changed ... | 1995 | 18470212 |
| molecular mapping of wheat. homoeologous group 3. | a prerequisite for molecular level genetic studies and breeding in wheat is a molecular marker map detailing its similarities with those of other grass species in the gramineae family. we have constructed restriction fragment length polymorphism maps of the a-, b-, and d-genome chromosomes of homoeologous group 3 of hexaploid wheat (triticum aestivum l. em. thell) using 114 f7-8 lines from a synthetic x bread wheat cross. the map consists of 58 markers spanning 230 cm on chromosome 3a, 62 marker ... | 1995 | 18470186 |
| molecular mapping of wheat. homoeologous group 2. | a molecular-marker map of bread wheat having many markers in common with other grasses in the gramineae family is a prerequisite for molecular level genetic studies and breeding in this crop species. we have constructed restriction fragment length polymorphism maps of the a-, b-, and d-genome chromosomes of homoeologous group 2 of hexaploid wheat (triticum aestivum l. em. thell) using 114 f7 lines from a synthetic x bread wheat cross and clones from 11 libraries. chromosomes 2a, 2b, and 2d compr ... | 1995 | 18470185 |
| characterization of hordeum chilense chromosomes by c-banding and in situ hybridization using highly repeated dna probes. | c-banding patterns of hordeum chilense and of triticum aestivum 'chinese spring' - h. chilense disomic addition lines were analyzed and compared with in situ hybridization patterns using a biotin-labeled highly repetitive triticum tauschii dna sequence, pas1, and a wheat 18s-26s rdna probe. all seven h. chilense chromosomes pairs and the added h. chilense chromosomes present in the addition lines were identified by their characteristic c-banding pattern. chromosome morphology and banding pattern ... | 1995 | 18470181 |
| introgression and characterization of barley yellow dwarf virus resistance from thinopyrum intermedium into wheat. | wheatgrasses (species of agropyron complex) have previously been reported to be resistant to barley yellow dwarf virus (bydv). to introgress this resistance into wheat, triticum aestivum x thinopyrum (agropyron) intermedium hybrids were advanced through a backcrossing program and reaction to bydv, as determined by enzyme-linked immunosorbent assay (elisa), is reported for the first time in backcross populations of wide hybrids between wheat and wheatgrasses. elisa values revealed highly resistan ... | 1995 | 18470179 |
| disomic thinopyrum intermedium addition lines in wheat with barley yellow dwarf virus resistance and with rust resistances. | zhong 5 is a partial amphiploid (2n = 56) between triticum aestivum (2n = 42) and thinopyrum intermedium (2n = 42) carrying all the chromosomes of wheat and seven pairs of chromosomes from th. intermedium. following further backcrossing to wheat, six independent stable 2n = 44 lines were obtained representing 4 disomic chromosome addition lines. one chromosome confers barley yellow dwarf virus (bydv) resistance, whereas two other chromosomes carry leaf and stem rust resistance; one of the latter ... | 1995 | 18470177 |
| detection of a 2.6 kb single/low copy dna sequence on chromosomes of wheat (triticum aestivum) and rye (secale cereale) by fluorescence in situ hybridization. | a fluorescence in situ hybridization procedure was developed to detect a 2.6 kb single/low copy dna sequence on chromosomes of wheat (triticum aestivum) and rye (secale cereale). the probe, pttksui26, was from the wheat genomic library generated at kansas state university and was labeled with bio-11-dutp by nick translation. the signal was amplified by a protocol of avidin-fitc (fluorescein isothiocyanate), biotinylated goat anti-avidin antibody, avidin-fitc. two rye chromosomes and several whea ... | 1995 | 18470163 |
| molecular genetic identification of avena chromosomes related to the group 1 chromosomes of the triticeae. | a collection of 19 wheat (triticum aestivum) probes, detecting sequences in the seven homoeologous groups of chromosomes, were hybridized to dna from the 'kanota' series of oat monosomic lines (avena byzantina) to investigate their use for identifying groups of homoeologous oat chromosomes. three probes from homoeologous group 1 of wheat, psr161, psr162, and psr121, mapped among the set of oat chromosomes 1c, 14, and 17. one homoeologous group 6 probe, psr167, mapped to oat chromosomes 1c and 17 ... | 1995 | 18470162 |
| reciprocal substitutions analysis of embryo induction and plant regeneration from anther culture in wheat (triticum aestivum l.). | reciprocal substitutions for all chromosomes between the hard red winter wheat cultivars wichita and cheyenne were used to investigate the effects of individual chromosomes, as well as their interactions with the genetic background, on androgenesis. duplicate lines for each chromosome were included to check background homogeneity. six experiments, two for each genome, were performed. in each experiment, 14 substitution lines, their 14 duplicate lines, and the two parental genotypes ('cheyenne' a ... | 1995 | 18470161 |
| molecular-genetic maps for group 1 chromosomes of triticeae species and their relation to chromosomes in rice and oat. | group 1 chromosomes of the triticeae tribe have been studied extensively because many important genes have been assigned to them. in this paper, chromosome 1 linkage maps of triticum aestivum, t. tauschii, and t. monococcum are compared with existing barley and rye maps to develop a consensus map for triticeae species and thus facilitate the mapping of agronomic genes in this tribe. the consensus map that was developed consists of 14 agronomically important genes, 17 dna markers that were derive ... | 1995 | 18470151 |
| genetic mapping in the 1r.1d wheat-rye translocated chromosomes. | translocation chromosomes 1r.1d5+10−1 and 1r.1d5+10−2 were produced to improve bread-making quality in triticale and to manipulate the dosage of the glu-d1 gene in wheat. they involve transfers of segments of the long arm of chromosome 1d of bread wheat to the long arm of rye chromosome 1r. the translocated long arms of the chromosomes were mapped genetically in wheat and triticale using polymorphism for c-banding patterns, allelic variation of the glu-d1 gene, and a telocentric chro ... | 1994 | 18470135 |
| cytological and molecular characterization of a triticum aestivum x lophopyrum ponticum backcross derivative resistant to barley yellow dwarf. | barley yellow dwarf is the most damaging virus-caused disease in bread wheat (triticum aestivum l.). a resistant line, sw335.1.2-13-11-1-5 (2n = 47), derived from a cross of t. aestivum x lophopyrum ponticum was characterized by meiotic chromosome pairing, by in situ dna hybridization and by expression of molecular markers to determine its chromosome constitution. all progeny of this line had three pairs of l. ponticum chromosomes from homoeologous chromosome groups 3, 5, and 6 and the 2n = 47 p ... | 1994 | 18470130 |
| rflp markers linked to powdery mildew resistance genes pm1, pm2, pm3, and pm4 in wheat. | near-isogenic lines (nils) and their recurrent parent chancellor (cc) were used to identify restriction fragment length polymorphic markers linked to powdery mildew (blumeria graminis (dc.) e.o. speer f.sp. tritici) resistance genes pm1, pm2, pm3, and pm4 in wheat (triticum aestivum l. em. thell). by mapping these polymorphic markers in f2 progenies from crosses of the nils with cc, it was found that pm1 cosegregated with a polymorphic locus detected by dna probe cdo347; pm2 was linked to a locu ... | 1994 | 18470129 |
| resistance to wheat leaf rust and stem rust in triticum tauschii and inheritance in hexaploid wheat of resistance transferred from t. tauschii. | twelve accessions of triticum tauschii (coss.) schmal. were genetically analyzed for resistance to leaf rust (puccinia recondita rob. ex desm.) and stem rust (puccinia graminis pers. f.sp. tritici eriks. and e. henn.) of common wheat (triticum aestivum l.). four genes conferring seedling resistance to leaf rust, one gene conferring seedling resistance to stem rust, and one gene conferring adult-plant resistance to stem rust were identified. these genes were genetically distinct from genes previo ... | 1994 | 18470124 |
| gene-for-gene interactions between the rye mildew fungus and wheat cultivars. | genetic mechanisms of the incompatibility between erysiphe graminis f.sp. secalis and wheat cultivars were analyzed using f1 hybrids between e. graminis f.sp. secalis, sk-1, and f.sp. tritici, tk-1. the avirulence of sk-1 on triticum aestivum 'norin 4', 'chinese spring', and 'kokeshi-komugi' was controlled by a single gene. the resistance of the three cultivars to sk-1 was also controlled by a single gene, pm15, a gene for resistance to e. graminis f.sp. agropyri. implications of these results w ... | 1994 | 18470120 |
| genetics of adult-plant leaf-rust resistance in four indian and two australian bread wheat cultivars. | genetic studies for leaf-rust resistance were conducted on four indian (cpan1235, hd2135, hp1209, and vl404) and two australian (csp44 and oxley) bread wheat cultivars. the f2 and f3 plants from their crosses with each other and with susceptible cultivar agra local were tested against a mixture of pathotypes 77-1 and 77-2 (variants of race 77). disease scores on f1's from resistant/susceptible parent crosses indicated partial dominance of resistance in these wheats. the six cultivars have two ad ... | 1994 | 18470088 |
| transfer of leaf rust and stem rust resistance genes from triticum triaristatum to durum and bread wheats and their molecular cytogenetic localization. | six accessions of triticum triaristatum (willd) godr. &gren. (syn. aegilops triaristata) (6x, uummunun), having good resistance to both leaf rust (puccinia recondita f.sp. tritici rob. ex desm) races and stem rust (p. graminis f.sp. tritici eriks. &henn.) races, were successfully crossed with both susceptible durum wheats (t. turgidum var. durum l., 2n = 28, aabb) and bread wheats (t. aestivum, 2n = 42, aabbdd). in some crosses, embryo rescue was necessary. the t. triaristatum resistance was exp ... | 1994 | 18470085 |
| interactions between the scs and vi genes in alloplasmic durum wheat. | two nuclear genes, vitality (vi) on an a- or b-genome chromosome and species cytoplasm specific (scs) on a 1dl telosome from triticum aestivum l. or a telosome from aegilops uniaristata vis. (un telosome), improved compatibility between the nucleus of triticum turgidum l. var. durum and the cytoplasm of ae. longissima s. &m. or ae. uniaristata. to study interactions between vi and scs and to determine the chromosomal location of vi, 29-chromosome fertile plants were crossed with 13 d-genome diso ... | 1994 | 18470070 |
| chromosomal localization of intergenomic rflp loci in hexaploid wheat. | hybridization of radiolabeled wheat dna probes to genomic dna digests of compensating nullisomic-tetrasomic lines and ditelosomic lines of hexaploid wheat (triticum aestivum l. cv. chinese spring) can be used to identify intergenomic rflps. sixty-three psti/bamhi genomic dna probes and eight cdna probes were used to determine the chromosomal locations of 223 dna fragments that define a minimum of 189 rflp loci. eighty-four percent of the genomic dna clones hybridize to fragments located in homoe ... | 1993 | 18470038 |
| the mechanism and inheritance of adult plant leaf rust resistance in 12 wheat lines. | twelve lines of wheat (triticum aestivum l.) were developed that had susceptible infection types to leaf rust (puccinia recondita rob. ex desm. f.sp. tritici) race un 15 in the seedling stage but were resistant in the adult plant stage in the field. the lines were developed from four crosses, each involving four parents (eight in total) that had originally been selected for adult plant or field resistance to stem rust (puccinia graminis pers. f.sp. tritici eriks, and henn.). the objectives of th ... | 1993 | 18470035 |
| reconstruction in wheat of complete chromosomes 1b and 1r from the 1rs.1bl translocation of 'kavkaz' origin. | translocation 1rs.1bl originally from the 'aurora' and 'kavkaz' wheats has spread to bread wheats all over the world. it offers several resistance genes and appears to significantly increase yields. for future study of the arm location of the yield-increasing factors, complete chromosomes 1b and 1r were reconstructed from the 1rs.1bl translocation. this was accomplished by centric misdivision and fusion in double monosomies 20 ii + 1rs.1bl i + 1bs.1rl i. the frequency of misdivision and misdivis ... | 1993 | 18470029 |
| gliadin alleles in canadian western red spring wheat cultivars: use of two different procedures of acid polyacrylamide gel electrophoresis for gliadin separation. | gliadin allele compositions of 21 canadian spring common wheat cultivars, most of which belong to the canada western red spring (cwrs) class, were studied and great similarity in their genotypes was confirmed. it was found that alleles frequent in the set of canadian wheats (such as gli-b1d, gli-d1j, gli-a2m, and gli-d2h) are very rare or absent in common wheat cultivars from other regions and countries studied earlier, indicating that germplasm of cwrs cultivars is rather unique. it may be sugg ... | 1993 | 18470021 |
| a cytogenetically based physical map of chromosome 1b in common wheat. | we have constructed a cytogenetically based physical map of chromosome 1b in common wheat by utilizing a total of 18 homozygous deletion stocks. it was possible to divide chromosome 1b into 17 subregions. nineteen genetic markers are physically mapped to nine subregions of chromosome 1b. comparison of the cytological map of chromosome 1b with an rflp-based genetic linkage map of triticum tauschii revealed that the linear order of the genetic markers was maintained between chromosome 1b of hexapl ... | 1993 | 18470009 |
| simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated dna probes. | common wheat, triticum aestivum, is an allohexaploid species consisting of three different genomes (a, b, and d). the three genomes were simultaneously discriminated with different colors. biotinylated total genomic dna of the diploid a genome progenitor triticum urartu, digoxigenin-labeled total genomic dna of the diploid d genome progenitor aegilops squarrosa, and nonlabeled total genomic dna of one of the possible b genome progenitors ae. speltoides were hybridized in situ to metaphase chromo ... | 1993 | 18470003 |
| combining mutations for the two homoeologous pairing suppressor genes ph1 and ph2 in common wheat and in hybrids with alien triticeae. | two lines of common wheat cv. chinese spring, carrying simultaneous mutations for the two major homoeologous pairing wheat suppressor genes ph1 and ph2 have been developed and their pairing behaviour compared with that of the ph1b mutant of the same cultivar. besides carrying the ph1b mutation, the first double mutant line lacked the chromosome arm pair 3ds, containing ph2, whereas the second had a euploid constitution and carried the ph1b allele on 3ds. hybrids of aegilops variabilis and secale ... | 1993 | 18469995 |
| alloplasmic wheat - elymus ciliaris chromosome addition lines. | alloplasmic euploid wheat with the cytoplasm of elymus ciliaris (2n = 4x = 28, scscycyc) is male sterile and has reduced vigor. however, alloplasmic plants with e. ciliaris chromosomes 1sc or 1yc marked by gliadin genes gli-sc1 and gli-ycl, respectively, are vigorous and fertile. the rf genes on 1sc and 1yc are named rf-sc1 and rf-yc1. two chromosome translocations involving 1yc were isolated. the first involved the short arm of 1yc translocated to the short arm of wheat chromosome 3b. the secon ... | 1993 | 18469991 |
| inheritance of leaf rust and stem rust resistance in 'roblin' wheat. | the canadian common wheat (triticum aestivum l.) cultivar 'roblin' is resistant to both leaf rust (puccinia recondita rob. ex. desm.) and stem rust (puccinia graminis pers. f. sp. tritici eriks. and e. henn.). to study the genetics of this resistance, 'roblin' was crossed with 'thatcher', a leaf rust susceptible cultivar, and rl6071, a stem rust susceptible line. a set of f6 random lines was developed from each cross. the random lines and the parents were grown in a field rust nursery artificial ... | 1993 | 18469988 |
| varying chromosome composition of 56-chromosome wheat x thinopyrum intermedium partial amphiploids. | thinopyrum intermedium (2n = 42) is a source of many potentially useful genes for wheat improvement. many partial amphiploids have been produced between th. intermedium and triticum aestivum that are fertile and stable. these partial amphiploids all have 56 chromosomes, including seven pairs of chromosomes from th. intermedium. to explore the genomic composition of these lines, meiotic analysis was conducted on 32 hybrid combinations between eight different partial amphiploids. all but two of th ... | 1993 | 18469982 |
| the effects of level of 2,4-d and time in culture on regeneration rate and chromosome numbers of regenerants from calli of the hybrid triticum aestivum cv. chinese spring ph1b x thinopyrum ponticum (2n = 10x = 70). | thinopyrum ponticum (podp.) barkworth &d. r. dewey (2n = 10x = 70) has excellent resistance for both leaf and stem rusts. long-term callus cultures were established from the immature embryos of a hybrid between triticum aestivum l. (2n = 6x = 42) x th. ponticum. they were maintained in culture for over 2 years and continued to grow and have organogenetic capacity. with increasing time on a maintenance medium, the plant regeneration rates of the hybrid calli decreased when transferred to regenera ... | 1993 | 18469979 |
| prediction of heterosis in wheat using coefficient of parentage and rflp-based estimates of genetic relationship. | genetic relationship can be a useful predictor of the relative performance of hybrid combinations for a hybrid breeding program resulting in reduced time and cost of hybrid testing. genetic relationships of 112 wheat (triticum aestivum l. em. thell.) lines were estimated using 41 dna clones hybridizing to 273 dna fragments and by calculating coefficient of parentage (cop). heterosis was estimated for 722 hybrids grown in multiple locations, with 189 being tested in more than 1 year. the average ... | 1996 | 18469962 |
| characterization of genomes and chromosomes in partial amphiploids of the hybrid triticum aestivum x thinopyrum ponticum by in situ hybridization, isozyme analysis, and rapd. | genomic in situ hybridization (gish) and southern hybridization of genome-specific rapd markers were used to demonstrate that the e genome (including ee and eb from thinopyrum elongatum and thinopyrum bessarabicum, respectively) and the st genome (from pseudoroegneria species) were the two basic genomes in thinopyrum ponticum. gish also revealed that the centromeric region may be the critical area that discriminates the st genome from the e genome in th. ponticum. of the seven partial amphiploid ... | 1996 | 18469955 |
| a gene for resistance to a necrosis-inducing isolate of pyrenophora tritici-repentis located on 5bl of triticum aestivum cv. chinese spring. | several sources of high-level resistance to tan spot caused by pyrenophora tritici-repentis have been identified in hexaploid wheat (triticum aestivum l.). this study was conducted to determine the number and chromosome location of a gene(s) in the cultivar chinese spring (cs) that confers resistance to a tan necrosis inducing isolate (nec+chl−) of p. tritici-repentis, 86-124, and insensitivity to ptr necrosis toxin. reciprocal crosses were made between cs (resistant-insensitive) and 'keny ... | 1996 | 18469918 |
| molecular evidence for triticum speltoides as a b-genome progenitor of wheat (triticum aestivum). | in polyploid wheat, the origin of the b-genome donor has remained relatively unknown in spite of a number of investigations attempting to identify the parental species. a project was designed to isolate and clone a genome-specific dna sequence from triticum speltoides l. to determine if that species could be the b-genome donor. a cloning scheme involving the prescreening of 1-kb fragments followed by colony, dot blot, and southern blot hybridization screenings was used to isolate a speltoides-sp ... | 1996 | 18469915 |
| analysis off cereal chromosomes by atomic force microscopy. | atomic force microscopy has been applied to the study of plant chromosomes from cereal grasses triticum aestivum (bread wheat), triticum tauschii, and hordeum vulgare (barley). using standard mitotic metaphase squashes, high resolution images have been obtained of untreated chromosomes and also of chromosomes after c-banding, n-banding, and in situ hybridization. the true 3-dimensional nature of the images permits detailed analysis of the surface structure and, on untreated uncoated chromosomes, ... | 1996 | 18469905 |
| molecular genetic maps of the group 6 chromosomes of hexaploid wheat (triticum aestivum l. em. thell.). | restriction fragment length polymorphism (rflp) maps of chromosomes 6a, 6b, and 6d of hexaploid wheat (triticum aestivum l. em. thell.) have been produced. they were constructed using a population of f7-8 recombinant inbred lines derived from a synthetic wheat x bread wheat cross. the maps consist of 74 markers assigned to map positions at a lod >= 3 (29 markers assigned to 6a, 24 to 6b, and 21 to 6d) and 2 markers assigned to 6d ordered at a lod of 2.7. another 78 markers were assigned to inter ... | 1996 | 18469899 |
| molecular cytogenetic analysis of agropyron chromatin specifying resistance to barley yellow dwarf virus in wheat. | nine families of bread wheat (tc5, tc6, tc7, tc8, tc9, tc10, tc14, 5395-(243aa), and 5395) with resistance to barley yellow dwarf virus and containing putative translocations between wheat and a group 7 chromosome of agropyron intermedium (l1 disomic addition line, 7ai#1 chromosome) induced by homoeologous pairing or tissue culture were analyzed. c-banding, genomic in situ hybridization (gish), and restriction fragment length polymorphism (rflp) in combination with repetitive agropyron-specific ... | 1996 | 18469897 |
| genome differentiation in aegilops. 1. distribution of highly repetitive dna sequences on chromosomes of diploid species. | genome differentiation in 12 diploid aegilops species was analyzed using in situ hybridization with the highly repetitive dna sequences psc119 and pas1 and c-banding. chromosomes of all these diploid aegilops species hybridized with the psc119 probe; however, the level of hybridization and labeling patterns differed among genomes. only four species (ae. squarrosa, ae. comosa, ae. heldreichii, and ae. uniaristata) showed distinct hybridization with pas1. the labeling patterns were species-specifi ... | 1996 | 18469894 |
| cytogenetic identification of triticum peregrinum chromosomes added to common wheat. | c-banded karyotypes of a complete set of 14 triticum peregrinum whole chromosome addition lines and 25 telosomic addition lines are reported. the added t. peregrinum chromosomes were not structurally rearranged compared with the corresponding chromosomes of the donor accession. comprehensive karyotypic analysis confirmed triticum umbellulatum as the donor species of the uv genome and identified triticum longissimum as the donor species of the sv genome of t. peregrinum. neither the uv nor sv gen ... | 1996 | 18469893 |
| identification, mapping, and application of polymorphic dna associated with resistance gene pm21 of wheat. | a new powdery mildew resistance gene designated pm21, from haynaldia villosa, a relative of wheat, has been identified and incorporated into wheat through an alien translocation line. cytogenetic and biochemical analyses showed that chromosome arms 6vs and 6al were involved in this translocation. random amplified polymorphic dna (rapd) analysis was performed on recipient wheat cultivar yangmai 5, the translocation line, and h. villosa with 180 random primers. eight of the 180 primers amplified p ... | 1996 | 18469886 |
| natural or induced nucleocytoplasmic heterogeneity in triticum longissimum. | alien cytoplasms produce a variety of phenotypes in durum wheat (triticum turgidum) and common wheat (triticum aestivum) cultivars, which indicate the prevalence of cytoplasmic variability in the subtribe triticinae. intraspecific cytoplasmic differences have been demonstrated between the subspecies of triticum speltoides, triticum dichasians, and triticum comosum. in this study, durum wheat lines with cytoplasm from two accessions, b and c, of triticum longissimum were compared, and meiotic chr ... | 1996 | 18469879 |
| characterization of an agropyron elongatum chromosome conferring resistance to cephalosporium stripe in common wheat. | related wheat (triticum aestivum l.) breeding lines, pi 561033, rea 9232, rea 9257, and ci 13113 were analyzed cytogenetically to characterize the association of resistance to cephalosporium stripe (caused by cephalosporium gramineum nis. & ika.) with agropyron elongatum chromatin. one pair of a. elongatum chromosomes was detected in pi 561033, rea 9232, and ci 13113 by genomic in situ hybridization. the sib line of pi 561033 and rea 9232, rea 9257, which is not resistant to this disease, lacked ... | 1996 | 18469877 |
| genotoxicity of triasulfuron in the wing spot test of drosophila melanogaster is modulated by winter wheat seedlings. | triasulfuron (ts) is a widely used sulfonylurea herbicide which inhibits the acetolactate synthase in broad-leaf weeds and in some wheat crop grasses (triticum aestivum l.). residues can be found in soil and superficial water with high toxicity to primary producers. in cereals, ts metabolism depends on cytochromes p450 (cyps), the age of seedlings and the interaction with compounds. the genotoxicity of ts was demonstrated in the wing spot test of drosophila melanogaster, an in vivo assay based o ... | 2008 | 18468945 |
| functional characterization of hfr1, a high-mannose n-glycan-specific wheat lectin induced by hessian fly larvae. | we previously cloned and characterized a novel jacalin-like lectin gene from wheat (triticum aestivum) plants that responds to infestation by hessian fly (mayetiola destructor) larvae, a major dipteran pest of this crop. the infested resistant plants accumulated higher levels of hfr-1 (for hessian fly-responsive gene 1) transcripts compared with uninfested or susceptible plants. here, we characterize the soluble and active recombinant his(6)-hfr1 protein isolated from escherichia coli. functiona ... | 2008 | 18467454 |
| structural organization of an alien thinopyrum intermedium group 7 chromosome in u.s. soft red winter wheat (triticum aestivum l.). | barley yellow dwarf virus (bydv) resistance in soft red winter wheat (srww) cultivars has been achieved by substituting a group 7 chromosome from thinopyrum intermedium for chromosome 7d. to localize bydv resistance, a detailed molecular genetic analysis was done on the alien group 7 th. intermedium chromosome to determine its structural organization. triticeae group 7 rflp markers and rye specific repetitive sequences used in the analysis showed that the alien chromosome in the p29 substitution ... | 1997 | 18464860 |
| root tip cell cycle synchronization and metaphase-chromosome isolation suitable for flow sorting in common wheat (triticum aestivum l.). | an efficient procedure for cell-cycle synchronization in meristematic root tips was achieved in common wheat. treatment parameters for synchronizing the cell cycle of root tip meristem cells, such as time-course and applied concentrations of various chemicals, were systematically tested and optimized by flow cytometric analysis of isolated nuclei. high mitotic indices (69.5% in the root tip meristematic area) were routinely obtained by treating germinating seeds with 1.25 mm hydroxyurea for 16 h ... | 1997 | 18464853 |
| identification of the entire chromosome complement of bread wheat by two-colour fish. | using the aegilops tauschii clone pas1 together with the barley clone phvg38 for two-colour fluorescence in situ hybridization (fish) the entire chromosome complement of hexaploid wheat was identified. the combination of the two probes allowed easy discrimination of the three genomes of wheat. the banding pattern obtained with the phvg38 probe containing the gaa-satellite sequence was identical to the n-banding pattern of wheat. a detailed idiogram was constructed, including 73 gaa bands and 48 ... | 1997 | 18464850 |
| construction of midget chromosomes in wheat. | to test the usefulness of breakage-fusion-bridge (bfb) cycles in generating new chromosome aberrations in bread wheat (triticum aestivum l.) and to extend the range of aberrations available, a series of midget chromosomes was produced from the long arm of chromosome 1b. using a reverse tandem duplication initiated chromatid type bfb cycle, the 1bl arm was broken and fused with centromeres of either chromosome 5bl or 1rs to form dicentric chromosomes. the 1r and 5b centromeres were broken by cent ... | 1997 | 18464847 |
| chromosome doubling of haploids of common wheat with caffeine. | treatment of dividing plant cells with caffeine inhibits their cytokinesis, thereby inducing the formation of binucleate cells that contain polyploid nuclei. this study was undertaken to determine whether caffeine treatments would induce chromosome doubling and seed set in haploids of common wheat (triticum aestivum l. em. thell.) through the further development of diploid nuclei. ten sterile wheat haploid plants, obtained through the agency of corn (zea mays l.) pollination, were multiplied by ... | 1997 | 18464846 |
| aflp fingerprinting reveals pattern differences between template dna extracted from different plant organs. | aflp (amplified fragment length polymorphism) fingerprinting of cultivars of bread wheat (triticum aestivum) and some of its wild relatives has allowed the efficient detection of large numbers of polymorphic amplified fragments. while the reproducibility of fingerprints in repeated experiments is high, pattern differences were observed between fingerprints obtained from seed and leaf dna template from the same wheat accession. these distinct organ specific amplified dna fragments were shown to b ... | 1997 | 18464843 |
| detection of quantitative trait loci associated with leaf rust resistance in bread wheat. | leaf rust, caused by puccinia recondita rob. ex desm., is a common disease in wheat. the objective of this study was to develop molecular markers associated with the quantitative trait loci (qtls) putatively conferring durable leaf rust resistance in triticum aestivum l. em. thell. a population of 77 recombinant inbred lines (rils) developed from 'parula' (resistant) and 'siete cerros' (moderately susceptible) was used. bulked segregant analysis was done using random amplified polymorphic dnas ( ... | 1997 | 18464825 |
| dna markers linked to a t10 loose smut resistance gene in wheat (triticum aestivum l.). | screening for loose smut resistance in wheat is difficult. selecting lines with dna markers linked to loose smut resistance would be more reliable and less costly. molecular markers linked to a race t10 loose smut resistance gene were identified using a f6 single seed descent segregating population. a rapd marker and a rflp marker were located on opposite flanks of the resistance gene and were shown to be loosely linked. the rapd marker was converted to a user friendly polymorphic scar marker th ... | 1997 | 18464816 |
| fumigation of wheat using liquid ethyl formate plus methyl isothiocyanate in 50-tonne farm bins. | australian standard white wheat, triticum aestivum l. (a marketing grade with mixed grain hardness),with a moisture content of 12.5% was fumigated with a new ethyl formate formulation (95% ethyl formate plus 5% methyl isothiocyanate) identified and developed by commonwealth scientific and industrial research organization entomology, canberra, australia. wheat was fumigated with the formulation at a calculated application rate of 80 g/m3 in two 50-tonne sealed metal vertical silos located at fish ... | 2008 | 18459432 |