Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| multiplication of mycobacterium leprae in normal mice. | 1987 | 3325572 | |
| endothelial cell bacillation in lepromatous leprosy: a case report. | 1987 | 3323754 | |
| the killing of mycobacterium leprae in mice by various dietary concentrations of clofazimine and ethionamide. | 1987 | 3323753 | |
| studies of reactivity of some sri lankan population groups to antigens of mycobacterium leprae. iii. the post-lepromin test scar in healthy populations in sri lanka. | 1987 | 3323752 | |
| persisting mycobacterium leprae among thelep trial patients in bamako and chingleput. subcommittee on clinical trials of the chemotherapy of leprosy (thelep) scientific working group of the undp/world bank/who special programme for research and training in tropical diseases. | 1987 | 3323750 | |
| [delayed cutaneous response to different antigens in household contacts of leprosy patients and controls]. | the delayed type hypersensitivity (dth) response to ppd, candidin and histoplasmin, and mitsuda reaction (mr) in household contacts (hc) of multibacillary (hcm) and paucibacillary (hcp) leprosy patients, and non exposed controls was studied. intradermal tests with 0.1 ml. of each antigen were performed and read after 48 hours. late nodular reaction to ml (mr) was evaluated at the 21st day post-challenge. as we had formerly demonstrated, mr was depressed in hcm. interestingly, this reaction was a ... | 1987 | 3323710 |
| [hansen i. considerations on its development]. | the causes of the existence and importance of the i form are discussed, as were as its characteristics and the advantage of using the letter i to refer to this group. it is called initial because it is the way the disease first appears and due to its epidemiological value. the method used in the research carried out in the department of dermatology of the pic on 444 patients with i form in the register of patients in the pic is 16%. the average age ranged from 30 to 39 years old. most of the pat ... | 1987 | 3323709 |
| dapsone susceptibility of m. leprae before and after 1977. | 1987 | 3323374 | |
| immunodiagnostic tests for leprosy; a need for standards. | 1987 | 3323373 | |
| serological reactivity and early detection of leprosy among contacts of lepromatous patients in cebu, the philippines. | 1987 | 3323372 | |
| appearance of a methoxy mycolate-like component by the acid methanolysis of mycobacterium leprae. | it has been reported that mycobacterium leprae contains two types of mycolic acid, namely, alpha- and keto-mycolic acids, thus it is taxonomically similar to m. bovis bcg. however, there was some controversy about the presence of methoxy mycolic acid which was observed in small amounts only in the case of experimentally infected (w45) armadillo-derived m. leprae. to investigate this fact, mycolic acids were extracted from the cell-wall structure of m. leprae and characterized using chromatograph ... | 1987 | 3323370 |
| primary and secondary dapsone resistance of m. leprae in martinique, guadeloupe, new caledonia, tahiti, senegal, and paris between 1980 and 1985. | primary and secondary dapsone resistance were studied among lepromatous patients living in martinique, guadeloupe, new caledonia, tahiti, senegal, and paris. four hundred fifteen biopsies were taken from clinically active and bacteriologically positive (bacterial index greater than 2) patients in the 6-year period of 1980-1985. among these, 280 biopsies that contained 5 x 10(4) acid-fast bacilli per ml with a morphological index of at least 0.10 were inoculated into the mouse foot pad, and 229 h ... | 1987 | 3323369 |
| effects of icrc antileprosy vaccine in healthy subjects. | long-term effects of the administration of the icrc antileprosy vaccine in healthy subjects have been investigated both in household contacts of leprosy patients and noncontacts in a general population. each volunteer received a dose of vaccine containing either 0.5 x 10(9) or 1.7 x 10(7) bacilli intradermally. the vaccine induces a dose-dependent lepromin conversion in negative subjects at 8 weeks after vaccination. one year later, the conversion rates are more than 90% in both high- and low-do ... | 1987 | 3323368 |
| antibodies to phenolic glycolipid-i during long-term therapy: serial measurements in individual patients. | levels of igm antibody to phenolic glycolipid-i (pgl-i) were measured in serum specimens collected over the initial 5 or more years of therapy from 11 leprosy patients. all three patients with paucibacillary disease had undetectable levels of antibody throughout their treatment. the eight patients with multibacillary disease had initially elevated levels which fell quite rapidly with treatment, reaching levels of 10% to 30% of their initial pre-treatment level after 5 years of therapy. the singl ... | 1987 | 3323367 |
| seroepidemiological study on 724 household contacts of leprosy patients in french polynesia using disaccharide-octyl-bsa as antigen. | a seroepidemiological surveillance of a contact population was started in 1984 in french polynesia. the elisa test was used to measure igm anti-nd-o-bsa in the sera. specific antibody levels were higher in healthy polynesians than in normal individuals living in a nonendemic country. the positive threshold of the reaction was fixed according to this background activity in healthy polynesians. under these conditions, 100% of the multibacillary patients were detected as seropositive as compared to ... | 1987 | 3323366 |
| antibodies to a synthetic analog of phenolic glycolipid-i of mycobacterium leprae in healthy household contacts of patients with leprosy. | fifty-four household contacts of lepromatous patients, 39 household contacts of tuberculoid patients, and 99 control persons were examined with an enzyme-linked immunosorbent assay for their antibody responses to phenolic glycolipid-i (pgl-i) of mycobacterium leprae using a synthetic analog (pgl-isa) with the same terminal sugar epitope, namely, o-(3, 6-di-o-methyl-beta-d-glucopyranosyl)-(1----4)-o-(alpha-l-rhamnopyranosyl )-(1----9)-oxynonanoyl-bsa. this study was conducted in the gurage area o ... | 1987 | 3323365 |
| autoaggressive hanseniasis. | i have given the name autoaggressive hanseniasis to a syndrome with immunopathologic and clinical pictures resembling autoaggressive systemic diseases observed in some cases of lepromatous and borderline hanseniasis. it is probably caused by b cell stimulation by antigenic complex of mycobacterium leprae plus autologous tissue, along with a dysfunction of the t-suppressor lymphocytes. | 1987 | 3323269 |
| an attempt to induce a histological enl symptom in m. leprae infected nude mice. | 1986 | 3323158 | |
| study of idiotypes expressed by monoclonal antibodies to the 35 kd and 12 kd antigens of mycobacterium leprae. | rabbit antisera were raised against four monoclonal antibodies (moab) binding with the 35 kd protein and four moab binding with the 12 kd protein antigen of mycobacterium leprae. antisera showed idiotype (id) specificity following cross-absorption with normal mouse globulin. one id on a single moab and another id shared between three moab were identified for each group. functional studies were carried out with the rb04 anti [anti-35 kd] specificity. the expression of this id and paratope in anti ... | 1987 | 3322615 |
| [various aspects of leprosy control in the developing countries of southeastern asia]. | 1987 | 3321772 | |
| analysis of the antigenic profile of mycobacterium leprae: cross-reactive and unique specificities of human and rabbit antibodies. | thirty-two mycobacterial components were detected by antibodies contained in leprosy patients' sera across the clinical spectrum and rabbit anti-m. leprae hyperimmune sera by western blot analysis of armadillo-derived m. leprae antigen preparations. sera of borderline tuberculoid patients were found to contain antibodies recognizing 18 m. leprae components. while the reactivity of the sera on the lepromatous pole seemed to be distributed over the entire molecular weight range, most of the reacti ... | 1987 | 3321410 |
| survey of primary dapsone-resistant leprosy. | 1986 | 3321049 | |
| adoptively transferred reactivity to m. leprae in nude mice infected with m. leprae. | reversal reactions are manifestations of delayed hypersensitivity to m. leprae and are thought to be usually accompanied by manifestations of effective cell-mediated immunity (cmi) as measured by bacterial clearing. these experiments were designed to study the induction of reversal reactions in m. leprae-infected, congenitally athymic nude mice using adoptive transfer of cmi. splenic cell suspensions derived from unimmunized heterozygous nu/+ mice, and those vaccinated with heat-killed m. leprae ... | 1987 | 3319298 |
| localization and retention of mycobacterial antigen in lymph nodes of leprosy patients. | although leprosy, a chronic disease caused by m. leprae, primarily affects skin and peripheral nerves, pathological changes and granulomas have been observed in lymph nodes which are: (a) present in tuberculoid lymph nodes in the absence of acid-fact bacilli and (b) persistent in lepromatous patients even after prolonged treatment. we detected substantial amounts of mycobacterial antigen in 16 leprous lymph nodes using anti-bcg by the peroxidase anti-peroxidase method. the load and distribution ... | 1987 | 3318906 |
| the nude mouse as an experimental lepromatous leprosy model (continued): the lepromatoid lesions in mystacial vibrissae located site of injection. | 1986 | 3316144 | |
| the experimental inoculation with mycobacterium leprae in the congenitally asplenic mouse. | 1986 | 3316143 | |
| vaccination of borderline tuberculoid leprosy patients with bcg plus killed mycobacterium leprae. | 1986 | 3316142 | |
| mycobacterium leprae surface components intervene in the early phagosome-lysosome fusion inhibition event. | bone marrow-derived cultured macrophages were infected with mycobacterium leprae. the bacteria were either used as freshly isolated organisms or incubated with m. leprae antiserum (1:5) for 30 min prior to phagocytosis. immediately after inoculation (1 to 4 h) and at 1 to 8 days later, macrophages were stained for acid phosphatase activity to assess fusions between phagosomes and lysosomes. inhibition of fusions was essentially apparent as an early event, which was partially reversed by antiseru ... | 1987 | 3316028 |
| serodiagnosis of leprosy with pgl-1 using elisa technique. | 1987 | 3315988 | |
| immunological effects of lepromin testing in sri lankan population groups. ii. effect on reactivity to a soluble protein antigen of mycobacterium leprae. | 1987 | 3312897 | |
| studies of reactivity of some sri lankan population groups to antigens of mycobacterium leprae. ii. reactivity to a soluble protein antigen. | 1987 | 3312896 | |
| primary dapsone resistance in bamako and chingleput: final report; thelep. subcommittee on clinical trials of the chemotherapy of leprosy (thelep) scientific working group of the undp/world bank/who special programme for research and training in tropical diseases. | 1987 | 3312895 | |
| the potential role of dna technology in leprosy. | 1987 | 3312894 | |
| most mycobacterium leprae carbohydrate-reactive monoclonal antibodies are directed to lipoarabinomannan. | each of more than 30 monoclonal antibodies that had been raised against mycobacterium leprae and previously classified as reactive with carbohydrate was shown to be directed against lipoarabinomannan, a prominent, highly pervasive, myo-inositol-phosphate-containing, cross-reactive antigen within the leprosy bacillus. some of the antibodies preferentially bound to the lipopolysaccharide of m. leprae rather than to that of mycobacterium tuberculosis, suggesting the presence of distinguishing struc ... | 1987 | 3312018 |
| human t cell proliferative responses to particulate microbial antigens are supported by populations enriched in dendritic cells. | the efficacy of dendritic cells in antigen presentation was studied in eight healthy subjects using a lymphoproliferation assay. both particulate (mycobacterium leprae, h37ra) and soluble (ppd, tetanus toxoid) bacterial antigens were used as stimulants over a concentration range of accessory cells (monocytes (mo) and dendritic cells (dc)) varying from 10 to 0.1% in co-cultures using t-enriched cells. in general, co-cultures with t + mo and t + dc at all concentrations of accessory cells showed s ... | 1987 | 3311498 |
| experimental leprosy in african green monkeys (cercopithecus aethiops): a model for polyneuritic leprosy. | three african green monkeys (cercopithecus aethiops) were inoculated intravenously and intracutaneously with mycobacterium leprae derived from a naturally infected mangabey monkey. all developed cutaneous lesions at inoculation sites. one developed disseminated cutaneous lesions, while the cutaneous lesions in the other two regressed and eventually disappeared. the animals were examined at necropsy five years after inoculation. all three had active leprosy infection in peripheral nerves with ext ... | 1987 | 3310689 |
| unexpected findings amongst the skin test responses to mycobacteria of bcg vaccinated kuwaiti school children. | a multiple skin test survey was carried out in kuwait on 1200 school children aged 8-11 years, and on 1228 children aged 12-16 years. with only 15 exceptions, all these children had received vaccination with japanese bcg just before they started school, 5 years and 9 years earlier respectively. tuberculin positivity was almost 90% in both groups, with a mean response size of 8.7 mm. this was associated with remarkably high responsiveness to many of the other mycobacterial species investigated. s ... | 1987 | 3310346 |
| peptidoglycan and arabinogalactan of mycobacterium leprae. | the arabinogalactan and peptidoglycan of armadillo-grown mycobacterium leprae were examined. within the limits defined by the small amount of material available, the resemblance of these polymers to those of other mycobacteria was confirmed. the polymers were linked by a highly acid-labile bond and the arabinogalactan was itself acid-labile; free arabinose and a variety of oligosaccharides containing both arabinose and galactose, as well as polysaccharide and peptidoglycan, were released by dilu ... | 1987 | 3309180 |
| detection of trehalose monomycolate in mycobacterium leprae grown in armadillo tissues. | trehalose-6-monomycolate (tmm) was isolated from the lipids of armadillo-derived mycobacterium leprae. only meagre amounts of this glycolipid were recovered, but its structure was unequivocally established. only alpha-mycolates were detected in the tmm by 252cf plasma desorption mass spectrometry. electron impact mass spectrometry showed the alpha branch to be principally c20. trehalose dimycolate (cord factor) was not detectable. since we have also found tmm in m. lepraemurium and in every myco ... | 1987 | 3309145 |
| exochelin-mediated iron acquisition by the leprosy bacillus, mycobacterium leprae. | exochelins, water-soluble siderophores of mycobacteria, were isolated and partially purified from culture filtrates of iron-deficiently grown cultures of mycobacterium neoaurum nctc 10439 and an armadillo-derived mycobacterium (adm 8563). two biologically active fractions mediating iron uptake were isolated from each bacterium which not only were able to transport iron into the producing organism but also into suspensions of mycobacterium leprae isolated from armadillo liver. the rate of exochel ... | 1987 | 3309144 |
| pyrolysis gas chromatography-mass spectrometry of mycobacterial mycolic acid methyl esters and its application to the identification of mycobacterium leprae. | pyrolysis gas chromatography-mass spectrometry of methyl mycolates from 32 species of mycobacteria, including mycobacterium leprae, was carried out. the mycobacteria could be classified into four groups in respect of the fatty acid ester patterns detected within the range c20 to c26. the applicability of this pyrolysis-gas chromatographic method for identifying m. leprae is discussed. | 1986 | 3309136 |
| microbe dependence of mycobacterium leprae: a possible intracellular relationship with protozoa. | 1987 | 3309094 | |
| schwann cells and m. leprae. | 1987 | 3309093 | |
| investigations into cultivation of m. leprae in a nasal mucus medium: a preliminary report. | 1987 | 3309092 | |
| an ultrastructural study of dermal nerves in early human leprosy. | skin biopsies from the cutaneous lesions of seven patients with indeterminate, bt, bl, and ll leprosy of less than 1 year's duration were examined by light and electron microscopy. inflammatory cells, which marked the location of mycobacterium leprae in bacilliferous cases (bl and ll) were most frequently and consistently found in relation to dermal blood vessels, neurovascular bundles, nerves, arrector pili muscles, and skin adnexa. the number of bacilli and inflammatory cells in the epineurium ... | 1987 | 3309090 |
| primary visceral virchowian (lepromatous) hanseniasis. | a case of primary visceral virchowian hanseniasis is presented. the onset and symptoms of the disease made one think that it was a lymphoma because of the severe enlargement of the liver, spleen, and lymph nodes. biopsies of the liver, lymph nodes, and bone marrow revealed virchowian infiltration with acid-fast bacilli and globi. the skin was free of lesions and negative to bacilli, and there were no neural symptoms. | 1987 | 3309089 |
| treatment of chronic erythema nodosum leprosum with cyclosporine a produces clinical and immunohistologic remission. | we have treated three leprosy patients suffering from chronic, steroid-dependent erythema nodosum leprosum (enl) with cyclosporine a (csa). excellent results were obtained in two patients. extra-cutaneous manifestations of the reactional state were completely suppressed, and the development of new skin lesions was sharply curtailed. immunohistologic abnormalities characteristic of active enl were corrected. lymphocyte subpopulations and anti-mycobacterial antibody levels in peripheral blood were ... | 1987 | 3309088 |
| metabolic studies on mycobacteria. v. a preliminary report on the atp synthesis by mycobacteria including m. leprae by using different substrates. | by deletion and addition of various substrates in sauton's and dubos media, an experimental system has been standardised in which the role of various nutrients in the energy synthesis of mycobacteria can be determined. by using this system with cultivable mycobacteria it was observed that glycerol and asparagine are the important ingredients for atp synthesis by mycobacteria. glucose further enhanced the atp synthesis and growth of these mycobacteria. in the media containing asparagine or glycer ... | 1987 | 3309086 |
| metabolic studies on mycobacteria. iii. demonstration of key enzymes of tca cycle in m. leprae. | cell free extracts of armadillo derived m. leprae, m. phlei, m. smegmatis and normal armadillo liver were analysed for the two key enzymes of tca cycle. aconitase activity was assayed in the presence of inhibitor fluorocitrate and it was observed that cell free extracts from cultivable mycobacteria as well as aramadillo derived m. leprae had this enzyme activity and 66-82% of this activity was inhibited by 0.1 mm fluorocitrate. 74% of m. leprae derived enzyme activity was inhibited by fluorocitr ... | 1987 | 3309085 |
| multi-drug therapy in leprosy. | 1987 | 3309084 | |
| vaccines in leprosy. | 1987 | 3309082 | |
| clinical applications of recent advances in immunology of leprosy. | 1987 | 3309081 | |
| limiting dilution analysis of the human t cell response to mycobacterial antigens from bcg vaccinated individuals and leprosy patients. | the number of peripheral blood t lymphocytes responding to soluble mycobacterial antigens from mycobacterium tuberculosis purified protein derivative (ppd) and m. leprae (mls) was estimated by limiting dilution analysis. antigen-induced lymphocyte activation was measured by means of [3h]tdr incorporation on day 10 of culture in the presence of suboptimal concentrations of interleukin 2 (il-2). in the peripheral blood of bcg-vaccinated individuals from the uk, the frequency of t lymphocytes respo ... | 1987 | 3308217 |
| suppression of delayed hypersensitivity skin reactions to tuberculin by m. leprae antigens in patients with lepromatous and tuberculoid leprosy. | delayed hypersensitivity skin reactions to tuberculin when injected alone or in mixture with antigens of m. leprae were examined in leprosy patients and in healthy controls. the tuberculin reaction was significantly inhibited in more than one half of both ll and bt patients by the soluble extract of m. leprae (leprosin), the leprosin derived 12 kd protein or leprosin depleted of the 12 kd antigen. however, suppression was not found in healthy controls from a leprosy endemic region. these results ... | 1987 | 3308210 |
| immunopurification of radiolabelled antigens of mycobacterium leprae and mycobacterium bovis (bacillus calmette-guerin) with monoclonal antibodies. | radiolabelled sonicate of mycobacterium leprae when examined by sds-page and two-dimensional gel electrophoresis (2-de) contained fewer antigens than the comparable sonicate from m. bovis (bacillus calmette-guerin) (bcg). a solid-phase immunopurification assay with anti-m. leprae monoclonal antibodies (moab) was used to characterize four of these antigens. three of the moab were m. leprae-specific and with them antigens with apparent molecular weights (mr) of 12,000 (12k), 18k, and 35k were isol ... | 1987 | 3306906 |
| [genetic component of the immune response to mycobacterium leprae in healthy subjects]. | 1986 | 3306256 | |
| [resistance of mycobacterium leprae to dapsone and rifampicin: apropos of a survey carried out in the cape verde region (senegal)]. | since 1983, primary resistance of m. leprae to dds and rifampicin has been evaluated in new cases of lepromatous leprosy observed in the cap-verde region in senegal. out of the 13 strains isolated, 10 (77%) have been found resistant to dds, 7 at low level, 2 at intermediate level, 1 at high level; all of them have been found sensible to rifampicin. similar results have been obtained with 57 strains isolated from patients not yet treated coming from different geographical areas, seeing that 37, i ... | 1987 | 3306248 |
| a cooperative taxonomic study of mycobacteria isolated from armadillos infected with mycobacterium leprae. | seventeen strains of mycobacteria, recovered from six armadillos experimentally infected with mycobacterium leprae, were examined in ten different laboratories. this collaborative study included use of conventional bacteriological tests, lipid analyses, determination of mycobactins and peptidoglycans, characterization by py-ms, and immunological, metabolic, pathological and dna studies. these armadillo-derived mycobacteria (adm) formed five homogeneous groups (numbered adm 1 to 5) on the basis o ... | 1986 | 3305778 |
| dominant cell wall proteins of mycobacterium leprae recognized by monoclonal antibodies. | a cell wall fraction of mycobacterium leprae has enhanced potency in activating immune t cells. by using a panel of monoclonal antibodies (moab), the dominant immunogen in this preparation was shown to be a complex of proteins of apparent molecular weight (mr) 65 to 50 kd with a major antigen of 65 kd. antigen capture assays supported the results of immunoblots and elisa that this protein was concentrated in the cell wall. by varying the moab used as capture or tracer antibody, one of the three ... | 1987 | 3304738 |
| the relationship between reactivities to lepromin a (fernandez and mitsuda) and a soluble protein antigen of mycobacterium leprae. | three methods of evaluation were used to investigate the relationship between skin test reactions elicited by different antigens of mycobacterium leprae. the latter were the fernandez and mitsuda reactions to lepromin, and that to a soluble protein antigen (spa) of m. leprae. all three methods of evaluation demonstrated some degree of relationship though not as high as would be expected. the closest correlation was between mitsuda and spa reactions; while fernandez and mitsuda, and fernandez and ... | 1987 | 3304321 |
| india carries out large-scale tests of anti-leprosy vaccine. | 1987 | 3302728 | |
| seroreactivity against the mycobacterium leprae phenolic glycolipid i in mycobacteria infected or stimulated groups of individuals. | 1987 | 3302578 | |
| studies of reactivity of some sri lankan population groups to antigens of mycobacterium leprae. i. reactivity to lepromin a. | 1987 | 3302577 | |
| bacterial index: ridley's vs. dharmendra's scale. | 1987 | 3302063 | |
| skin smear examination in relation to multidrug therapy campaigns. | 1987 | 3302062 | |
| seroepidemiological studies in subsided cases of multi and paucibacillary types of leprosy using fla-abs test. | fla-abs test was carried out in 145 subsided cases of multibacillary (bl/ll) and 197 subsided cases of paucibacillary (tt/bt/i) types. the period of subsidence varied from 0-5 years or more. it was observed that percentage positivity decreases with the subsidence of the disease. in multibacillary cases, fla-abs positivity was 95% (active cases), 50% (after 1 year of subsidence) and 36% (after 5 years of subsidence) whereas in paucibacillary types fla-abs positivity was 80% (active cases), 34% (a ... | 1987 | 3302060 |
| transmission of leprosy. | 1987 | 3302059 | |
| concepts behind the development of multiple drug therapy regimens in leprosy. | 1986 | 3301333 | |
| chemical synthesis of the trisaccharide unit of the species-specific phenolic glycolipid from mycobacterium leprae. | o-(3,6-di-o-methyl-beta-d-glucopyranosyl)-(1----4)-o-(2,3-di-o-methyl- alpha-l-rhamnopyranosyl)-(1----2)-3-o-methyl-l-rhamnopyranose, the haptenic trisaccharide of the mycobacterium leprae-specific phenolic glycolipid i (pgl-i) antigen, and related trisaccharides, were synthesized by allylation of o-2 of benzyl 4-o-benzyl-alpha-l-rhamnopyranoside using phase-transfer catalysis, methylation of the product, deallylation, and coupling to o-(2,4-di-o-acetyl-3,6-di-o-methyl-beta-d-glucopyranosyl)-(1- ... | 1987 | 3300972 |
| rapid in vitro metabolic screen for antileprosy compounds. | measurement of intracellular atp of mycobacterium leprae after direct in vitro exposure to antimicrobial agents was evaluated as a rapid means of identifying potentially useful therapeutic agents. nude mouse-derived m. leprae was incubated in an axenic modified dubos medium in the presence or absence of antimicrobial agents for up to 3 weeks. atp was then assayed by using the firefly bioluminescence technique. rifampin, clofazimine, and ethionamide each effected a significantly accelerated rate ... | 1987 | 3300539 |
| primary dapsone resistance in multibacillary leprosy among nepalese children. | 1987 | 3299724 | |
| leprosy: cause, transmission, and a new theory of pathogenesis. | leprosy is generally accepted as being caused by mycobacterium leprae, an acid-fast organism often present in great numbers in certain forms of leprosy. however, it has not been possible to confirm with scientifically acceptable evidence that this entity is the cause of leprosy; laboratory cultivation, an essential factor in the proof, has not been accomplished with the acid-fast bodies seen in leprotic tissue. the mechanisms of transmission of the disease also remain conjectural; prolonged, clo ... | 1987 | 3299638 |
| environmental nonhuman sources of leprosy. | leprosy has been considered to occur only after exposure to a human case. however, evidence has been accumulating that this conventional view is wrong and that an environmental nonhuman source is critical to some human infections with mycobacterium leprae. observations, some of which date back to the nineteenth century, support soil, vegetation, water, arthropods, and armadillos (dasypus novemcinctus) as environmental sources of leprosy. disparate clinical, epidemiologic, and microbiologic evide ... | 1987 | 3299637 |
| [testing of the footpad technic for the cultivation of mycobacterium leprae in different lines of mice populations in cuba]. | 1986 | 3299546 | |
| characteristics of patients in the thelep trials of chemotherapy of leprosy at bamako and chingleput. subcommittee on clinical trials of the chemotherapy of leprosy (thelep) scientific working group of the undp/world bank/who special programme for research and training in tropical diseases. | 1987 | 3298905 | |
| affinity of mycobacterium leprae with lewis rat schwannoma cell line (lewis tc 98). | 1987 | 3298903 | |
| mycobacterium leprae, probably a microbe-dependent microorganism. | 1987 | 3298484 | |
| effect of preformed immune complexes on the course of mycobacterium leprae infection in normal mice. | 1987 | 3298483 | |
| an unusual case of untreated lepromatous leprosy with rare bacilli: an immunologic follow-up. | 1987 | 3298482 | |
| effect of interleukin-1 (il-1) and il-2 on lymphocytes from patients with leprosy. | 1987 | 3298481 | |
| activity of selected beta-lactam antibiotics against mycobacterium leprae. | twelve beta-lactam antibiotics were tested for activity against mycobacterium leprae growing in the foot pads of mice. two cephalosporins (7-aminocephalosporanic acid and cefuroxime) and one cephamycin (cefoxitin) showed significant activity against m. leprae, and one penicillin (mezlocillin) exerted possible growth-promoting activity. these results suggest that particular molecular structures may be required for activity against m. leprae. | 1987 | 3298478 |
| ability of the phenolic glycolipid-i antigen of m. leprae to elicit a positive mitsuda response in the armadillo (dasypus novemcinctus). | three lepromin-positive armadillos and three lepromin-negative armadillos were tested intradermally with 100 micrograms of phenolic glycolipid-i (pgl-i) in 0.1 ml of normal saline. positive delayed-hypersensitivity granulomas at 21 days in the lepromin-positive animals and negative responses in the lepromin-negative animals were obtained. these observations suggest that purified pgl-i is capable of eliciting cell-mediated immune or delayed-hypersensitivity responses in animals sensitized to myco ... | 1987 | 3298476 |
| immunization of mice with mycobacterium leprae/mycobacterium bovis bcg admixtures: modulation of the acquired response to bcg. | the generation of acquired immunity to bcg in mice was compared to that in animals receiving an admixture of bcg and killed mycobacterium leprae. no significant qualitative differences were observed between the two groups in terms of their generation of delayed sensitivity, of protective t cells, and of immunological memory. in addition, the admixture was as effective as bcg in conferring protective immunity against certain nontuberculous mycobacteria, and in one case, that of m. avium 706, sign ... | 1987 | 3298475 |
| sensitivity and specificity of the fla-abs test for leprosy in mexican populations. | the epidemiological surveillance for leprosy must include several clinical and laboratory procedures. the fla-abs test of abe could be a useful tool for this purpose because it allows the demonstration of an effective contact with mycobacterium leprae. in order to establish the specificity, sensitivity, and predictability of the fla-abs test under mexican conditions, we studied sera collected from six groups of individuals: 60 healthy donors from a nonendemic area, 57 cases hospitalized for cond ... | 1987 | 3298474 |
| defective monocyte chemotaxis in active lepromatous leprosy. | this study of monocyte chemotaxis in leprosy patients showed a significant inverse correlation (p less than 0.05) of chemotaxis and the bacterial index (bi) (n = 22). in addition, there was a significant inverse correlation (p less than 0.05) between chemotaxis and the serum levels of anti-phenolic glycolipid-i igm antibodies (n = 20). patients taking thalidomide who had a bi greater than or equal to 1 had a significantly greater (p less than 0.001) chemotaxis response than that of patients with ... | 1987 | 3298473 |
| assessment of the immune deficit in leprosy patients and the effect of recombinant il-2 in vitro. | although the mechanism of immunologic unresponsiveness in lepromatous leprosy remains unknown, it has been shown that interleukin-2 (il-2) production is defective in these patients. peripheral blood mononuclear cells (pbmc) were isolated from treated (less than 16 months) and untreated leprosy patients as well as household contacts; age, sex, ethnically matched control subjects; and laboratory staff. pbmc were cultured for 6 days with sonicated mycobacterium leprae (1-10 micrograms/ml), dharmend ... | 1987 | 3298472 |
| activity of minocycline in mycobacterium leprae-infected mice. | 1987 | 3298454 | |
| mycobacterial antigen detection by immunohistochemistry in pulmonary tuberculosis. | a preliminary diagnosis of tuberculosis can be established by the detection of acid-fast bacilli (afb) and confirmed by culture of the microorganism. to evaluate an alternative method of diagnosis, the distribution of mycobacterial antigens in lung tissue specimens was characterized by an indirect peroxidase-antiperoxidase method and was compared to the detection of afb by ziehl-neelsen stain. histologic specimens were obtained from 59 hospital patients. of nine patients with mycobacterial disea ... | 1987 | 3297995 |
| beta-lactamase synthesis in mycobacterium leprae. | beta-lactam antibiotics are not active against mycobacterium leprae. the enzyme beta-lactamase mediates the most common form of bacterial resistance to penicillins and cephalosporins. cell-free extracts of purified suspensions of m. leprae were examined for beta-lactamase. the bacteria were prepared from the tissues of experimentally-infected nine-banded armadillos. most of the suspensions were inactive. however, the bacteria obtained from the tissues of armadillos treated with penicillin g benz ... | 1987 | 3295489 |
| concepts behind the development of multiple drug therapy regimens in leprosy. | 1986 | 3295437 | |
| surveillance for naturally acquired leprosy in a nine-banded armadillo population. | samples from 77 nine-banded armadillos (dasypus novemcinctus) inhabiting a 16.7 km portion of the east atchafalaya river levee, point coupee parish, louisiana, were serologically tested and/or histopathologically examined for evidence of naturally acquired leprosy. five of 67 (7.5%) armadillos tested sero-positive with elisa test for igm class antibodies to the phenolic-glycolipid-1 antigen of mycobacterium leprae. one of 74 (1.3%) was histopathologically positive as determined by presence of ac ... | 1987 | 3295310 |
| characteristics of the multiplication of dapsone-resistant strains of mycobacterium leprae in mice. subcommittee on clinical trials of the c hemotherapy of leprosy (thelep) scientific working group of undp/world bank/who special programme for research and training in tropical diseases. | 1988 | 3294541 | |
| association of igg and igm antibodies to phenolic glycolipid-1 antigen of mycobacterium leprae with disease parameters in multibacillary leprosy patients. | 1988 | 3294540 | |
| ultrastructure of human foetal schwann cells in tissue culture infected with mycobacterium leprae. | 1988 | 3294539 | |
| screening of drug resistant strains of mycobacterium leprae in lepromatous leprosy patients under multidrug treatment. | 1988 | 3294170 | |
| human monoclonal antibodies to phenolic glycolipid-i derived from patients with leprosy, and production of specific anti-idiotypes. | human monoclonal antibodies (mab) were produced by hybridomas derived from fusion of the gm4672 lymphoblastoid cell line and peripheral blood mononuclear cells from leprosy patients. hybridoma supernatants were screened for immunoglobulin (ig) secretion, binding to mycobacterium leprae, phenolic glycolipid-i (phen gl-i), the unique m. leprae glycolipid and single-stranded(ss)dna by elisa. on the basis of direct-binding elisas, two igmk mab (pr4 and th3) were selected for characterization. pr4 an ... | 1988 | 3292409 |
| biophysical optima for metabolism of mycobacterium leprae. | the metabolic response of freshly harvested, nude-mouse-derived mycobacterium leprae to biophysical parameters was studied to facilitate an understanding of axenic culture requirements. quantitation of intracellular atp and the rate of [u-14c]palmitic acid incorporation into phenolic glycolipid i (pgl-i) were used as metabolic indicators after axenic incubation in modified dubos medium under various biophysical conditions. pgl-i synthesis was optimal at 33 degrees c, whereas atp was optimally ma ... | 1988 | 3290244 |
| antigens of mycobacterium leprae identified by immunoprecipitation with sera from leprosy and tuberculosis patients. | mycobacterial antigens which react with human b lymphocytes were investigated by immunoprecipitation of radiolabelled sonicates of mycobacterium leprae and m. bovis (bcg) with sera from patients with leprosy and tuberculosis in the presence of staphylococcus aureus. sds-page analysis of the immunoprecipitates demonstrated that dense bands of mr 12,000 (12k), 15k, 27k, 32-33k, 36k and 48k were the major antigens of m. leprae recognized by antibodies in lepromatous leprosy sera. of these, only the ... | 1988 | 3289801 |
| chemotherapy of leprosy. | 1988 | 3289485 | |
| [case of lepromatous leprosy]. | 1988 | 3289278 |