Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [anti-cytokine activity of microorganisms]. | development of a method of determination of anti-cytokine activity (aca) of microorganisms, study of the prevalence and intensity of aca to pro- and anti-inflammatory cytokines in pathogenic and opportunistic bacteria. | 2011 | 21913393 |
| innate immune control of salmonella enterica serovar typhimurium: mechanisms contributing to combating systemic salmonella infection. | infections with salmonella enterica serovars remain a serious problem worldwide. while serovar typhi causes significant morbidity and mortality that is restricted to humans, serovar typhimurium causes gastroenteritidis in humans and can also infect other animals. as mice with the susceptible nramp1 locus get systemic infection with serovar typhimurium, murine infection models using this serovar have been widely used to decipher the immune mechanisms required to survive systemic salmonella infect ... | 2011 | 21912097 |
| Phenotypic and molecular characterization of human salmonella enterica serovar 4,[5],12:i:- isolates in Slovakia. | Forty-three epidemiologically unrelated emerging Salmonella enterica subsp. enterica serovar 4,[5],12:i:- strains isolated during the period 2009-2010 in Slovakia were characterized by phenotypic and genotypic methods. Thirty-one isolates (72.1%) expressed resistance to ampicillin, streptomycin, sulfizoxazole, and tetracycline [R-type ASSuT]. The majority of the strains belonged to both definitive phage types DT193 (30.2%) and U311 (27.9%). Other phage types identified were U302 (6.9%), DT18 (4. ... | 2011 | 21909783 |
| the transcript from the σ(28)-dependent promoter is translationally inert in the expression of the σ(28)-encoding gene flia in the fliaz operon of salmonella enterica serovar typhimurium. | there are three classes of promoters for flagellar operons in salmonella. class 2 promoters are transcribed by σ(70) rna polymerase in the presence of an essential activator, flhd(4)c(2), and activated by an auxiliary regulator, fliz. class 3 promoters are transcribed by σ(28) rna polymerase and repressed by an anti-σ(28) factor, flgm. σ(28) (flia) and fliz are encoded by the flia and fliz genes, respectively, which together constitute an operon transcribed in this order. this operon is transcri ... | 2011 | 21908664 |
| evaluation of an immunochromatographic assay for rapid detection of salmonella enterica serovars typhimurium and enteritidis. | an immunochromatographic assay was developed to detect salmonella enterica serovars typhimurium and enteritidis in a single strip. the assay was constructed in the form of a sandwich, using 2 specific anti-s. typhimurium and anti-s. enteritidis antibodies immobilized on a nitrocellulose membrane at separated test lines, while the other specific antibody to salmonella spp. was conjugated with gold nanoparticles. the test strips can immediately detect s. typhimurium and s. enteritidis specifically ... | 2011 | 21908327 |
| Trichinella spiralis: intranasal immunization with attenuated Salmonella enterica carrying a gp43 antigen-derived 30mer epitope elicits protection in BALB/c mice. | Trichinellosis is a public health problem and is considered an emergent/re-emergent disease in various countries. The etiological agent of trichinellosis is the nematode Trichinella, which infects domestic animals such as pigs and horses, as well as wild animals and humans. A veterinary vaccine could be an option to control the disease in domestic animals. Although several vaccine candidates have shown promising results, a vaccine against trichinellosis remains unavailable to date. Attenuated Sa ... | 2011 | 21907709 |
| Use of bioinformatic SNP predictions in differentially expressed genes to find SNPs associated with Salmonella colonization in swine. | Asymptomatic Salmonella-carrier pigs present a major problem in preharvest food safety, with a recent survey indicating >50% of swine herds in the United States have Salmonella-positive animals. Salmonella-carrier pigs serve as a reservoir for contamination of neighbouring pigs, abattoir pens and pork products. In addition, fresh produce as well as water can be contaminated with Salmonella from manure used as fertilizer. Control of Salmonella at the farm level could be through genetic improvemen ... | 2011 | 21906181 |
| Reduction of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes with electrolyzed oxidizing water on inoculated hass avocados (Persea americana var. Hass). | This study was intended to evaluate the bactericidal effect of electrolyzed oxidizing water (EOW) and chlorinated water on populations of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes inoculated on avocados (Persea americana var. Hass). In the first experiment, inoculated avocados were treated with a water wash applied by spraying tap water containing 1 mg/liter free chlorine for 15 s (WW); WW treatment and then spraying sodium hypochlorite in water containing 75 mg/l ... | 2011 | 21902927 |
| Prevalence of Salmonella enterica and the hygienic indicator Escherichia coli in raw meat at markets in Ouagadougou, Burkina Faso. | This study investigated the hygienic status and prevalence of Salmonella and Escherichia coli in retail meat sold at open markets in Ouagadougou, Burkina Faso. A total of 150 samples of beef meat (n = 45), beef intestine (n = 45), mutton (n = 30), and chicken (n = 30) were collected from four local markets for investigation. The prevalence of Salmonella enterica subsp. enterica was 9.3%, and six serotypes, all previously unreported in Burkina Faso, were identified: Derby, Tilene, Hato, Bredeney, ... | 2011 | 21902926 |
| comparison of two possible routes of pathogen contamination of spinach leaves in a hydroponic cultivation system. | the route of pathogen contamination (from roots versus from leaves) of spinach leaves was investigated with a hydroponic cultivation system. three major bacterial pathogens, escherichia coli o157:h7, salmonella, and listeria monocytogenes, were inoculated into the hydroponic solution, in which the spinach was grown to give concentrations of 10⁶ and 10³ cfu/ml. in parallel, the pathogens were inoculated onto the growing leaf surface by pipetting, to give concentrations of 10⁶ and 10³ cfu per leaf ... | 2011 | 21902924 |
| inhibition of growth of pathogenic bacteria in raw milk by legume protein esters. | protein isolates from soybean and chickpea, as well as their methylated esters, were tested for their inhibitory action against the propagation of pathogenic bacteria in raw milk during its storage either at room temperature or under refrigeration. raw milk was inoculated with a mixed culture of listeria monocytogenes scott a and salmonella enterica serovar enteritidis strain pt4 at ca. 2 log cfu ml⁻¹. aerobic plate count, coliform count, and presumptive e. coli in raw milk treated with esterifi ... | 2011 | 21902916 |
| Prevalence and antimicrobial susceptibility of major foodborne pathogens in imported seafood. | Seafood is a leading commodity implicated in foodborne disease outbreaks in the United States. Seafood importation rose dramatically in the past 3 decades and now contributes to more than 80% of the total U.S. seafood supply. However, limited data are available on the microbiological safety of imported seafood. In this study, we obtained a total of 171 salmon, shrimp, and tilapia samples imported from 12 countries in three retail stores in Baton Rouge, LA. The total microbial population and the ... | 2011 | 21902913 |
| Salmonella effectors: important players modulating host cell function during infection. | Salmonella enterica serovar Typhimurium (S. Typhimurium) is a Gram-negative facultative food-borne pathogen that causes gastroenteritis in humans. This bacterium has evolved a sophisticated machinery to alter host cell function critical to its virulence capabilities. Central to S. Typhimurium pathogenesis are two Type III secretion systems (T3SS) encoded within pathogenicity islands SPI-1 and SPI-2 that are responsible for the secretion and translocation of a set of bacterial proteins termed eff ... | 2011 | 21902796 |
| Application of a bacteriophage cocktail to reduce Salmonella Typhimurium U288 contamination on pig skin. | Multidrug-resistant Salmonella Typhimurium U288 is a significant pathogen of pigs, accounting for over half of all outbreaks on UK pig production premises. The potential of this serovar, and other salmonellae, to enter the food chain during the slaughtering process requires that efforts be made to reduce the prevalence of these bacteria at both the pre- and post-harvest stages of production. A bacteriophage cocktail (PC1) capable of lysing various Salmonella enterica serovars was designed using ... | 2011 | 21899907 |
| characterization of a vii-like phage specific to escherichia coli o157:h7. | phage vb_ecom_cba120 (cba120), isolated against escherichia coli o157:h7 from a cattle feedlot, is morphologically very similar to the classic phage vii of salmonella enterica serovar typhi. until recently, little was known genetically or physiologically about the vii-like phages, and none targeting e. coli have been described in the literature. the genome of cba120 has been fully sequenced and is highly similar to those of both vii and the shigella phage ag3. the core set of structural and repl ... | 2011 | 21899740 |
| The ERM protein, ezrin, regulates neutrophil transmigration by modulating the apical localization of MRP2 in response to the SipA effector protein during Salmonella Typhimurium infection. | In human disease induced by Salmonella enterica serovar Typhimurium (S. Typhimurium), transepithelial migration of neutrophils rapidly follows attachment of the bacteria to the epithelial apical membrane. We have previously shown that during S. Typhimurium infection the multidrug resistance-associated protein 2 (MRP2) is highly expressed at the apical surface of the intestinal epithelia, and that it functions as an efflux pump for the potent neutrophil chemoattractant hepoxilin A(3) . However, t ... | 2011 | 21899702 |
| purification and characterization of the acetyl-coa synthetase from mycobacterium tuberculosis. | acetyl-coa (accoa) synthetase (acs) catalyzes the conversion of acetate into accoa, which is involved in many catabolic and anabolic pathways. although this enzyme has been studied for many years in many organisms, the properties of mycobacterium tuberculosis acs and the regulation of its activity remain unknown. here, the putative acs gene of m. tuberculosis h37rv (mt-acs) was expressed as a fusion protein with 6×his-tag on the c-terminus in escherichia coli. the recombinant mt-acs protein was ... | 2011 | 21896569 |
| A new biphenyl and antimicrobial activity of extracts and compounds from Clusia burlemarxii. | Phytochemical investigation on Clusia burlemarxii (Clusiaceae) led to isolation and identification of nine compounds. Were isolated from leaves 3-O-a-L- rhamnopyranosylquercetin, 3-O-a-L-rhamnopyranosylkaempferol, 4-hydroxy-5,5-dimethyldihydrofuran-2-one, 2Z-d-tocotrienoloic acid and friedelin and were isolated from trunk betulinic acid, protocatechuic acid, lyoniresinol, and a new biphenyl 2,2-dimethyl-3,5-dihydroxy-7-(4-hydroxyphenyl)chromane. The structures were determined by ¹H, ¹³C-NMR, DEP ... | 2011 | 21893172 |
| associations among pathogenic bacteria, parasites, and environmental and land use factors in multiple mixed-use watersheds. | over a five year period (2004-08), 1171 surface water samples were collected from up to 24 sampling locations representing a wide range of stream orders, in a river basin in eastern ontario, canada. water was analyzed for cryptosporidium oocysts and giardia cyst densities, the presence of salmonella enterica subspecies enterica, campylobacter spp., listeria monocytogenes, and escherichia coli o157:h7. the study objective was to explore associations among pathogen densities/occurrence and objecti ... | 2011 | 21889781 |
| Characterization of bla(CMY)-Encoding Plasmids Among Salmonella Isolated in the United States in 2007. | Abstract Salmonella enterica is one of the most common bacterial causes of foodborne illness, and nontyphoidal Salmonella is estimated to cause ~1.2 million illnesses in the United States each year. Plasmids are mobile genetic elements that play a critical role in the dissemination of antimicrobial resistance determinants. AmpC-type CMY ß-lactamases (bla(CMY)) confer resistance to extended-spectrum cephalosporins and ß-lactam/ß-lactamase inhibitor combinations and are commonly plasmid-encoded. ... | 2011 | 21883005 |
| tlr5-dependent immunogenicity of a recombinant fusion protein containing an immunodominant epitope of malarial circumsporozoite protein and the flic flagellin of salmonella typhimurium. | recently, we described the improved immunogenicity of new malaria vaccine candidates based on the expression of fusion proteins containing immunodominant epitopes of merozoites and salmonella enterica serovar typhimurium flagellin (flic) protein as an innate immune agonist. here, we tested whether a similar strategy, based on an immunodominant b-cell epitope from malaria sporozoites, could also generate immunogenic fusion polypeptides. a recombinant his6-tagged flic protein containing the c-term ... | 2011 | 21881771 |
| defining treatment conditions for pulsed electric field pasteurization of apple juice. | the influence of temperature and the presence of n(α)-lauroyl ethylester (ethyl lauroyl arginate, lae) on the inactivation caused by continuous pulsed electric field treatments (pef) in escherichia coli o157:h7 suspended in apple juice have been investigated to define treatment conditions applicable at industrial scale that promote an equivalent safety level when compared with thermal processing. in the range of experimental conditions investigated (outlet temperature: 20-40 °c, electric field s ... | 2011 | 21880388 |
| the stepone real-time polymerase chain reaction detection of salmonella sp., salmonella enterica ser. typhimurium and enteritidis in milk and meat. | the aim of this study was to follow contamination of ready to eat milk and meat products with salmonella spp. by using the stepone real-time polymerase chain reaction (pcr). classical microbiological methods for detection of foodborne bacteria involve the use of pre-enrichment and/or specific enrichment, following isolation of bacteria in solid media and the final confirmation by biochemical and/or serological tests. we used the prepseq rapid spin sample preparation kit for isolation of dna and ... | 2011 | 21879831 |
| Salmonella bongori provides insights into the evolution of the Salmonellae. | The genus Salmonella contains two species, S. bongori and S. enterica. Compared to the well-studied S. enterica there is a marked lack of information regarding the genetic makeup and diversity of S. bongori. S. bongori has been found predominantly associated with cold-blooded animals, but it can infect humans. To define the phylogeny of this species, and compare it to S. enterica, we have sequenced 28 isolates representing most of the known diversity of S. bongori. This cross-species analysis al ... | 2011 | 21876672 |
| the deubiquitinase activity of the salmonella pathogenicity island 2 effector, ssel, prevents accumulation of cellular lipid droplets. | to cause disease, salmonella enterica serovar typhimurium requires two type iii secretion systems that are encoded by salmonella pathogenicity islands 1 and 2 (spi-1 and -2). these secretion systems serve to deliver specialized proteins (effectors) into the host cell cytosol. while the importance of these effectors to promote colonization and replication within the host has been established, the specific roles of individual secreted effectors in the disease process are not well understood. in th ... | 2011 | 21875964 |
| Identification of environmental reservoirs of nontyphoidal salmonellosis: aptamer-assisted bioconcentration and subsequent detection of salmonella typhimurium by quantitative polymerase chain reaction. | In this study, identification of environmental reservoirs of Salmonella enterica subsp. enterica serovar Typhimurium (abbreviated as Salmonella Typhimurium) in sediments, water, and aquatic flora collected from the Ganges River (Ganges riverine material) was carried out by adopting a two-step strategy. Step 1 comprised a selective serovar-specific capture of Salmonella Typhimurium from potential reservoirs. Step 2 involved culture-free detection of selectively captured Salmonella Typhi ... | 2011 | 21875107 |
| Standardisation of multilocus variable-number tandem-repeat analysis (MLVA) for subtyping of Salmonella enterica serovar Enteritidis. | Salmonella enterica serovar (S.) Enteritidis is an important cause of food-borne infection in Europe and the United States. Further subtyping of isolates is necessary to support epidemiological data for the detection of outbreaks and identification of the vehicle of infection. Multilocus variable-number tandem-repeat analysis (MLVA) is reportedly more discriminatory and produces data that are easier to share via databases than other molecular subtyping methods. However, lack of standardisation o ... | 2011 | 21871223 |
| improved templated fluorogenic probes enhance the analysis of closely related pathogenic bacteria by microscopy and flow cytometry. | templated fluorescence activation has recently emerged as a promising molecular approach to detect and differentiate nucleic acid sequences in vitro and in cells. here, we describe the application of a reductive quencher release strategy to the taxonomic analysis of gram-negative bacteria by targeting a single nucleotide difference in their 16s rrna in a two-color assay. for this purpose, it was necessary to develop a release linker containing a quencher suitable for red and near-infrared fluoro ... | 2011 | 21870777 |
| change in antimicrobial resistance pattern in salmonella enterica serovar typhimurium isolates detected in a beef cattle farm. | multidrug-resistant salmonella enterica serovar typhimurium (s. typhimurium) isolates with four different antimicrobial resistance patterns obtained from a beef cattle farm were characterized to determine their clonality. macrorestriction analysis of genomic dna revealed that these four isolates are closely related to each other and can be classified as a newly emerged pulsed-field gel electrophoresis type among cattle: cluster vii. three of the four isolates showed resistance to extended-spectr ... | 2011 | 21869573 |
| TBK1 mediates crosstalk between the innate immune response and autophagy. | The autophagic pathway participates in many physiological and pathophysiological processes. Autophagy plays an important role, as part of the innate immune response, in the first line of defense against intruding pathogens. Recognition of pathogens by the autophagic machinery is mainly mediated by autophagic adaptors, proteins that simultaneously interact with specific cargos and components of the autophagic machinery. However, the exact mechanisms and signaling pathways regulating this step are ... | 2011 | 21868362 |
| Study of the chemical composition and antimicrobial activities of ethanolic extracts from roots of Scutellaria baicalensis Georgi. | Scutellaria baicalensis Georgi (SBG), commonly named Huangqin, showed strong in vitro antimicrobial effects. However, limited research is available to systematically evaluate the effects of extraction methods on the phytochemical composition of SBG and its associated antimicrobial effects. In addition, limited studies have tested SBG as a natural antimicrobial agent on fresh produce such as tomatoes. In the current study, powered roots of SBG were extracted with 60, 80, and 100% ethanol, and th ... | 2011 | 21866919 |
| MLVA polymorphism of Salmonella enterica subspecies isolated from humans, animals, and food in Cambodia. | ABSTRACT: | 2011 | 21861934 |
| [surveillance of nalidixic acid-resistant and extended-spectrum beta-lactamase-producing salmonella spp. isolated from human feces]. | nalidixic acid (na)-resistant and extended-spectrum beta-lactamase (esbl)-producing salmonella sp. isolates from human specimens are associated with clinical failure or delayed response in subjects treated with fluoroquinolone or third-generation cephalosporins. we studied drug susceptibility in 604 salmonella enterica isolates from human feces in 2007. of these, 39 (6.5%) were resistat to na. of these, 46% were resistant to two or more drugs and 2% susceptible to na were resistant to multiple d ... | 2011 | 21861438 |
| [Development of modified molecular beacon based real-time PCR assay for the rapid detection of Salmonella choleraesuis and Salmonella paratyphi C]. | To develop real-time PCR assay based on modified molecular beacon for simultaneous detection of S. choleraesuis and S. paratyphi C. The established method was applied to the rapid detection of S. choleraesuis in food and stool samples of food poisoning, and then was applied to the identification of Salmonella C. | 2011 | 21861361 |
| arma methyltransferase in a monophasic salmonella enterica isolate from food. | the 16s rrna methyltransferase arma is a worldwide emerging determinant that confers high-level resistance to most clinically relevant aminoglycosides. we report here the identification and characterization of a multidrug-resistant salmonella enterica subspecies i.4,12:i:- isolate recovered from chicken meat sampled in a supermarket on february 2009 in la reunion, a french island in the indian ocean. susceptibility testing showed an unusually high-level resistance to gentamicin, as well as to am ... | 2011 | 21859937 |
| SadA, a trimeric autotransporter from Salmonella enterica serovar Typhimurium, can promote biofilm formation and provides limited protection against infection. | Salmonella enterica is a major cause of morbidity worldwide and mortality in children and immunocompromised individuals in sub-Saharan Africa. Outer membrane proteins of Salmonella are of significance because they are at the interface between the pathogen and the host, they can contribute to adherence, colonization, and virulence, and they are frequently targets of antibody-mediated immunity. In this study, the properties of SadA, a purported trimeric autotransporter adhesin of Salmonella enteri ... | 2011 | 21859856 |
| Genome sequencing reveals diversification of virulence factor content and possible host adaptation in distinct subpopulations of Salmonella enterica. | Divergence of bacterial populations into distinct subpopulations is often the result of ecological isolation. While some studies have suggested the existence of Salmonella enterica subsp. enterica subclades, evidence for these subdivisions has been ambiguous. Here we used a comparative genomics approach to define the population structure of Salmonella enterica subsp. enterica, and identify clade-specific genes that may be the result of ecological specialization. | 2011 | 21859443 |
| the role of rama on the development of ciprofloxacin resistance in salmonella enterica serovar typhimurium. | active efflux pump is a primary fluoroquinolone resistant mechanism of clinical isolates of salmonella enterica serovar typhimurium. rama is an essential element in producing multidrug resistant (mdr) s. enterica serovar typhimurium. the aim of the present study was to elucidate the roles of rama on the development of ciprofloxacin, the first choice for the treatment of salmonellosis, resistance in s. enterica serovar typhimurium. spontaneous mutants were selected via several passages of s. ente ... | 2011 | 21858134 |
| hydrogen-stimulated carbon acquisition and conservation in salmonella enterica serovar typhimurium. | salmonella enterica serovar typhimurium can utilize molecular hydrogen for growth and amino acid transport during anaerobic growth. via microarray we identified h(2) gas-affected gene expression changes in salmonella. the addition of h(2) caused altered expression of 597 genes, of which 176 genes were upregulated and 421 were downregulated. the significantly h(2)-upregulated genes include those that encode proteins involved in the transport of iron, manganese, amino acids, nucleosides, and sugar ... | 2011 | 21856852 |
| methodologies for salmonella enterica subsp. enterica subtyping: gold standards and alternatives. | for more than 80 years, subtyping of salmonella enterica has been routinely performed by serotyping, a method in which surface antigens are identified based on agglutination reactions with specific antibodies. the serotyping scheme, which is continuously updated as new serovars are discovered, has generated over time a data set of the utmost significance, allowing long-term epidemiological surveillance of salmonella in the food chain and in public health control. conceptually, serotyping provide ... | 2011 | 21856826 |
| The C-terminal domain of the Salmonella enterica WbaP (UDP-galactose:Und-P galactose-1-phosphate transferase) is sufficient for catalytic activity and specificity for undecaprenyl monophosphate. | Two families of membrane enzymes catalyze the initiation of the synthesis of O-antigen lipopolysaccharide. The Salmonella enterica Typhimurium WbaP is a prototypic member of one of these families. We report here the purification and biochemical characterization of the WbaP C-terminal (WbaP(CT)) domain harboring one putative transmembrane helix and a large cytoplasmic tail. An N-terminal thioredoxin fusion greatly improved solubility and stability of WbaP(CT) allowing us to obtain highly purified ... | 2012 | 21856724 |
| Three novel Anas platyrhynchos avian ß-defensins, upregulated by duck hepatitis virus, with antibacterial and antiviral activities. | Three novel Anas platyrhynchos avian ß-defensins (Apl_AvBDs), Apl_AvBD4, 7 and 12, were identified successfully and characterized in tissues from Peking ducks in the present study. The cDNA fragment of Apl_AvBD4 contained 171 bp, and encoded 56 amino acids. The complete nucleotide sequences of Apl_AvBD7 and 12 contained 204 bp and 198 bp open reading frames, which encoded 67 and 65 amino acids, respectively. Both recombinant and synthetic forms of the three Apl_AvBDs showed antibacterial activit ... | 2011 | 21856003 |
| direct feeding of microencapsulated bacteriophages to reduce salmonella colonization in pigs. | salmonella shedding often increases in pigs after transportation and/or lairage. we previously showed that administering anti-salmonella bacteriophages to pigs by gavage significantly reduced salmonella colonization when the pigs were exposed to a salmonella-contaminated holding pen. here we tested whether a microencapsulated phage cocktail would remain effective if the treatment was administered to pigs in the feed. pigs (n=21) were randomly placed into three groups: feed, gavage, and control. ... | 2011 | 21854261 |
| Characterization of Salmonella occurring at high prevalence in a population of the land iguana Conolophus subcristatus in Galápagos Islands, Ecuador. | The aim of the study was to elucidate the association between the zoonotic pathogen Salmonella and a population of land iguana, Colonophus subcristatus, endemic to Galápagos Islands in Ecuador. We assessed the presence of Salmonella subspecies and serovars and estimated the prevalence of the pathogen in that population. Additionally, we investigated the genetic relatedness among isolates and serovars utilising pulsed field gel electrophoresis (PFGE) on XbaI-digested DNA and determined the antimi ... | 2011 | 21853080 |
| Single nucleotide polypmorphisms of fimH associated with adherence and biofilm formation by serovars of Salmonella enterica. | Type 1 fimbriae produced by serovars of Salmonella are characterized by their ability to agglutinate guinea pig erythrocytes in the absence of d-mannose but not in its presence. The FimH protein is the adhesin that mediates this reaction; it is distinct from the major fimbrial protei.n (FimA) that composes the fimbrial shaft. Avian-adapted serovars of Salmonella produce non-haemagglutinating fimbriae that have been reported to mediate adherence to avian cells. A single amino acid substitution is ... | 2011 | 21852351 |
| Involvement of red blood cells in the regulation of leukotriene synthesis in polymorphonuclear leucocytes upon interaction with Salmonella Typhimurium. | Leukotriene (LT) B4 is the primary eicosanoid product of polymorphonuclear leucocytes (PMNLs). We studied LT synthesis in PMNLs upon interaction with Salmonella enterica serovar Typhimurium. Human PMNLs exposed to Salmonella produced LTs; mostly LTB4 and ?-hydroxy-LTB4. Opsonization with normal serum increased the capacity of S. Typhimurium to induce LT synthesis in PMNLs. Addition of red blood cells (RBCs) alone did not activate LT synthesis in PMNLs but did further increase the Salmonella-indu ... | 2011 | 21851422 |
| salmonella enterica serotype typhimurium usurps the scaffold protein iqgap1 to manipulate rac1 and mapk signalling. | salmonella enterica serotype typhimurium invades eukaryotic cells by re-arranging the host-cell cytoskeleton. however, the precise mechanisms by which salmonella induces cytoskeletal changes remain undefined. iqgap1 (iq motif-containing gtpase-activating protein 1) is a scaffold protein that binds multiple proteins including actin, the rho gtpases rac1 and cdc42 (cell division cycle 42), and components of the mapk (mitogen-activated protein kinase) pathway. we have shown previously that optimal ... | 2011 | 21851337 |
| Response surface methodology-based optimization of decontamination conditions for Escherichia coli O157:H7 and Salmonella Typhimurium on fresh-cut celery using thermoultrasound and calcium propionate. | A combination of thermoultrasound (temperature: 50, 55, 60°C; time: 10, 15, 20 min with the frequency of 40 KHz) and calcium propionate (concentration: 1, 2, 3%, w/v) treatment was applied to decontaminate Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium (S. Typhimurium) from fresh-cut celery. Using a Box-Behnken experimental design, predictive quadratic equations were developed for treatment-based population reductions of E. coli O157:H7 (R(2)=0.98, p<0.001) and S. Typhimuri ... | 2011 | 21849219 |
| evaluation of abelmoschus moschatus extracts for antioxidant, free radical scavenging, antimicrobial and antiproliferative activities using in vitro assays. | abelmoschus moschatus medik. leaves and seeds are considered as valuable traditional medicine. the aromatic seeds of this plant are aphrodisiac, ophthalmic, cardio tonic, antispasmodic and used in the treatment of intestinal complaints and check queasiness. to give a scientific basis for traditional usage of this medicinal plant, the seed and leaf extracts were evaluated for their antioxidant, free radical scavenging, antimicrobial and antiproliferative activities. | 2011 | 21849051 |
| nifedipine affects the course of salmonella enterica serovar typhimurium infection by modulating macrophage iron homeostasis. | background.ôçâiron overload can adversely influence the course of infection by increasing microbial replication and suppressing antimicrobial immune effector pathways. recently, we have shown that the calcium channel blocker nifedipine can mobilize tissue iron in mouse models of iron overload. we therefore investigated whether nifedipine treatment affects the course of infection with intracellular bacteria via modulation of iron homeostasis. methods. the effect of nifedipine on intramacrophage r ... | 2011 | 21844295 |
| immunization with an anti-idiotypic antibody against the broadly lipopolysaccharide-reactive antibody wn1 222-5 induces escherichia coli r3-core-type specific antibodies in rabbits. | the mouse monoclonal antibody (mab) wn1 222-5 recognizes a carbohydrate epitope in the inner core region of lps that is shared by all strains of escherichia coli and salmonella enterica and is able to neutralize their endotoxic activity in vitro and in vivo. immunization of mice with mab wn1 222-5 yielded several anti-idiotypic mabs one of which (mab s81-19) competitively inhibited binding of mab wn1 222-5 to e. coli and salmonella lps. after immunization of rabbits with mab s81-19, the serologi ... | 2011 | 21844130 |
| physico-chemical properties of salmonella typhi vi polysaccharide-diphtheria toxoid conjugate vaccines affect immunogenicity. | in this study it was demonstrated that the immunogenicity of vi polysaccharide-diphtheria toxoid conjugates was related to the physical and chemical structure of the conjugate. conjugates were prepared in two steps, firstly binding adipic acid dihydrazide (adh) spacer molecules to diphtheria toxoid (dt) carrier protein then secondly binding varying amounts of this derivatized dt to a fixed amount of vi capsular polysaccharide purified from salmonella enterica serovar typhi. as the amount of dt b ... | 2011 | 21843575 |
| comparative genomics and phylogeny of the inci1 plasmids: a common plasmid type among porcine enterotoxigenic escherichia coli. | increasing reports of multidrug resistance conferred by conjugative plasmids of enterobacteriaceae necessitate a better understanding of their evolution. one such group is the narrow-host-range inci1 plasmid type, known for their ability to carry genes encoding resistance to extended-spectrum beta lactamases. the focus of this study was to perform comparative sequencing of inci1 plasmids from porcine enterotoxigenic escherichia coli (etec), isolated irrespective of antimicrobial susceptibility p ... | 2011 | 21843549 |
| management of quinolone-resistant typhoid osteomyelitis. | 2011 | 21841594 | |
| microarray based analysis of inca/c plasmid associated genes from multidrug resistant salmonella enterica. | in enterobacteriaceae, plasmids have been classified according to 27 incompatibility (inc) or replicon types which are based on the inability of different plasmids with the same replication mechanism to co-exist in the same cell. certain replicon types such as inca/c are associated with multidrug resistance (mdr). we developed a microarray that contains 286 unique 70 mer oligonucleotide probes based on sequences from five inca/c plasmids: pyr1 (yersinia ruckeri), ppip1202 (yersinia pestis), pp99 ... | 2011 | 21841024 |
| anti-inflammatory properties of probiotic bacteria on salmonella-induced il-8 synthesis in enterocyte-like caco-2 cells. | invasion of the gut by pathogenic salmonella leads to production of il-8 that initiates inflammatory reactions to combat the bacterium. however, its persistent production causes tissue damage and interventions that suppress il-8 production prevent tissue damage. we hypothesised that probiotics could mediate their benefits via inhibition of il-8 synthesis. caco-2 cells were infected with probiotic bifidobacterium infantis w52, lactobacillus casei w56, lactococcus lactis w58, lactobacillus acidoph ... | 2010 | 21840800 |
| protective effect by bacillus smithii tbmi12 spores of salmonella serotype enteritidis in mice. | avoiding food-borne diseases by competitive exclusion agents is a proactive strategy. in the current paper, we report the use of bacillus smithii tbmi12 spores as potential competitive exclusion agents. one group of mice was predosed for three successive days with 10(8) colony forming units of b. smithii tbmi12 spores followed by inoculation with 10(6) colony forming units of wild-type salmonella enterica serotype enteritidis cells. microbial plate counts of the animals' livers and spleens showe ... | 2010 | 21840796 |
| occurrence of incfii plasmids carrying the bla(ctx-m-15) gene in salmonella enterica serovar enteritidis sequence type 11 in korea. | a total of 49 consecutive nonduplicate salmonella isolates collected in a nationwide survey performed in 2009 in korea were included in this study. resistance gene, sizes, and replicon sequence types (rsts) of r plasmids, and sequence types (sts) and xbai-macrorestriction patterns of extended-spectrum +¦-lactamase (esbl)-producing isolates were determined. six salmonella enterica serovar enteritidis isolates of st11 showed esbl phenotypes, and the isolates harbored an incompatibility group fii p ... | 2011 | 21840672 |
| evaluation of salmonella enterica serovar typhimurium and choleraesuis slya mutant strains for use in live attenuated oral vaccines. | slya protein plays a key role in virulence in salmonella enterica. in this study, we evaluated the ability of the slya mutant strain of s. enterica serovar choleraesuis (s. choleraesuis) to protect against swine salmonellosis. using a murine model infected with s. enterica serovar typhimurium (s. typhimurium), we showed that the salmonella strain with a deletion of slya could be used as a highly immunogenic, effective and safe vaccine in mice. based on these data, a slya mutant of s. enterica se ... | 2011 | 21840053 |
| investigation of diversity of plasmids carrying the blatem-52 gene. | objectives to investigate the diversity of plasmids that carry bla(tem-52) genes among escherichia coli and salmonella enterica originating from animals, meat products and humans. methods a collection of 22 bla(tem-52)-encoding plasmids was characterized by restriction fragment length polymorphism (rflp), replicon typing (by pcr or replicon sequencing), susceptibility testing, assessment of plasmid ability to self-transfer by conjugation and typing of the genetic environment of the bla(tem-52) g ... | 2011 | 21831988 |
| adaptation to the chicken intestine in salmonella enteritidis pt4 studied by transcriptional analysis. | the transcriptional changes that occurred in salmonella enterica serovar enteritidis during colonization of the alimentary tract of newly hatched chickens were studied. a whole genome oligonucleotide microarray was used to compare the expression pattern with that from bacteria cultured in nutrient broth in vitro. amongst other changes salmonella pathogenicity island (spi)-1, spi-2 and spi-5 genes were up-regulated in vivo suggesting a close association with the mucosa during colonization. partic ... | 2011 | 21831537 |
| exposure of salmonella enterica serovar typhimurium to high level biocide challenge can select multidrug resistant mutants in a single step. | biocides are crucial to the prevention of infection by bacteria, particularly with the global emergence of multiply antibiotic resistant strains of many species. concern has been raised regarding the potential for biocide exposure to select for antibiotic resistance due to common mechanisms of resistance, notably efflux. | 2011 | 21829527 |
| recombination and population structure in salmonella enterica. | salmonella enterica is a bacterial pathogen that causes enteric fever and gastroenteritis in humans and animals. although its population structure was long described as clonal, based on high linkage disequilibrium between loci typed by enzyme electrophoresis, recent examination of gene sequences has revealed that recombination plays an important evolutionary role. we sequenced around 10% of the core genome of 114 isolates of enterica using a resequencing microarray. application of two different ... | 2011 | 21829375 |
| The Cost of Virulence: Retarded Growth of Salmonella Typhimurium Cells Expressing Type III Secretion System 1. | Virulence factors generally enhance a pathogen's fitness and thereby foster transmission. However, most studies of pathogen fitness have been performed by averaging the phenotypes over large populations. Here, we have analyzed the fitness costs of virulence factor expression by Salmonella enterica subspecies I serovar Typhimurium in simple culture experiments. The type III secretion system ttss-1, a cardinal virulence factor for eliciting Salmonella diarrhea, is expressed by just a fraction of t ... | 2011 | 21829349 |
| a salmonella typhimurium-typhi genomic chimera: a model to study vi polysaccharide capsule function in vivo. | the vi capsular polysaccharide is a virulence-associated factor expressed by salmonella enterica serotype typhi but absent from virtually all other salmonella serotypes. in order to study this determinant in vivo, we characterised a vi-positive s. typhimurium (c5.507 vi(+)), harbouring the salmonella pathogenicity island (spi)-7, which encodes the vi locus. s. typhimurium c5.507 vi(+) colonised and persisted in mice at similar levels compared to the parent strain, s. typhimurium c5. however, the ... | 2011 | 21829346 |
| how salmonella oxidises h(2) under aerobic conditions. | salmonella enterica serovar typhimurium is a gram negative bacterial pathogen and a common cause of food-borne illness. molecular hydrogen has been shown to be a key respiratory electron donor during infection and h(2) oxidation can be catalysed by three genetically-distinct [nife] hydrogenases. of these, hydrogenases-1 (hyd-1) and hyd-2 have well-characterised homologues in escherichia coli. the third, designated hyd-5 here, is peculiar to salmonella and is expressed under aerobic conditions. i ... | 2011 | 21827758 |
| characterization of d-boroala as a novel broad spectrum antibacterial agent targeting d-ala-d-ala ligase. | d-boroala was previously characterized as an inhibitor of bacterial alanine racemase and d-ala-d-ala ligase enzymes [duncan, k., et al biochemistry 1989, 28:3541-9]. in the present study, d-boroala was identified and characterized as an antibacterial agent. d-boroala has activity against both gram-positive and gram-negative organisms, with mics down to 8 ++g/ml. a structure-function study on the alkyl side chain (nh(2) -chr-b(or')(2) ) revealed that d-boroala is the most effective agent in a ser ... | 2011 | 21827632 |
| [osteomyelitis of the costae caused by salmonella.] | salmonella enterica serotype enteritidis was detected in a 34-year-old, previously healthy patient who suffered from osteomyelitis of the costae. radical surgical debridement and antibiotic therapy were able to stop the infection. an abdominal flap and a thoracic flap were used to cover the soft tissue defect. | 2011 | 21826496 |
| impairment of swimming motility by antidiarrheic lactobacillus acidophilus strain lb retards internalization of salmonella enterica serovar typhimurium within human enterocyte-like cells. | we report that the culture and cell-free culture supernatant (cfcs) of l. acidophilus lb have the ability: (i) to delay the appearance of s. typhimurium sl1344-induced mobilization of f-actin; and (ii) subsequently, to retard the cell-entry of s. typhimurium sl1344. time-lapse imaging and western immunoblotting showed that the s. typhimurium sl1344 swimming motility cell-track types were rapidly but temporarily blocked without affecting the expression of flic flagellar propeller protein. we show ... | 2011 | 21825295 |
| tammar wallaby mammary cathelicidins are differentially expressed during lactation and exhibit antimicrobial and cell proliferative activity. | cathelicidins secreted in milk may be central to autocrine feedback in the mammary gland for optimal development in addition to conferring innate immunity to both the mammary gland and the neonate. this study exploits the unique reproductive strategy of the tammar wallaby (macropus eugenii) model to analyse differential splicing of cathelicidin genes and to evaluate the bactericidal activity and effect of the protein on mammary epithelial cell proliferation. two linear peptides, con73 and con218 ... | 2011 | 21824524 |
| a nationwide outbreak of salmonella bovismorbificans associated with sprouted alfalfa seeds in finland, 2009. | salmonella enterica serotype bovismorbificans is a rare serotype in finland. in june 2009, a nationwide outbreak of s. bovismorbificans infections occurred, and 42 clinical isolates were identified. we conducted a case-control study enrolling 28 cases and 48 matched controls, and found ready-to-eat alfalfa sprouts associated with the infection (odds ratioôçâ=ôçâ35.2, 95% confidence interval 2.8-435). the sprouts were traced back to a domestic producer, with the seeds originating in italy. althou ... | 2011 | 21824360 |
| foodborne outbreak of salmonella subspecies iv infections associated with contamination from bearded dragons. | approximately 1.4 million salmonella infections and 400 deaths occur annually in the united states. approximately 6% of human salmonella cases are thought to be associated with reptiles; salmonella enterica subspecies iv is primarily reptile-associated. during 1-4 december, 2009, three isolates of salmonella iv 6,7:z4,z24:- with indistinguishable pulsed-field gel electrophoresis (pfge) patterns were identified through minnesota department of health laboratory-based surveillance. none of the thre ... | 2011 | 21824356 |
| Prevalence of Salmonella, Yersinia and Campylobacter spp. in Feral Raccoons (Procyon lotor) and Masked Palm Civets (Paguma larvata) in Japan. | To estimate the public and animal health risk that alien species pose, the prevalence of Salmonella, Yersinia, and Campylobacter spp. in feral raccoons (Procyon lotor, nÔÇâ=ÔÇâ459) and masked palm civets (Paguma larvata, nÔÇâ=ÔÇâ153), which are abundant alien species in Japan, was investigated in urban and suburban areas of Japan. Salmonella enterica was detected from 29 samples [26 raccoons, 5.7%, 95% confidence interval (CI) 7.8-3.5%; three masked palm civets, 2.0%, 95% CI 4.2-0%]. Many of the ... | 2011 | 21824337 |
| salmonella exploits arl8b-directed kinesin activity to promote endosome tubulation and cell-to-cell transfer. | the facultative intracellular pathogen salmonella enterica serovar typhimurium establishes a replicative niche, the salmonella-containing vacuole (scv), in host cells. here we demonstrate that these bacteria exploit the function of arl8b, an arf family gtpase, during infection. following infection, arl8b localized to scvs and to tubulated endosomes that extended along microtubules in the host cell cytoplasm. arl8b(+) tubules partially colocalized with lamp1 and scamp3. formation of lamp1(+) tubu ... | 2011 | 21824248 |
| the n-terminal region of the medium subunit (pdud) packages adenosylcobalamin-dependent diol dehydratase (pducde) into the pdu microcompartment. | salmonella enterica produces a proteinaceous microcompartment for b(12)-dependent 1,2-propanediol utilization (pdu mcp). the pdu mcp consists of catabolic enzymes encased within a protein shell and its function is to sequester propionaldehyde, a toxic intermediate of 1,2-propanediol degradation. here, we report that a short n-terminal region of the medium subunit (pdud) is required for packaging the coenzyme b(12)-dependent diol dehydratase (pducde) into the lumen of the pdu mcp. analysis of sol ... | 2011 | 21821773 |
| modification of technical properties of lactobacillus johnsonii ncc 533 by supplementing growth medium with unsaturated fatty acids. | the aim of this study was to investigate the influence of supplementing growth media with unsaturated fatty acids on the technical properties of the probiotic strain lactobacillus johnsonii ncc 533, such as heat and acid tolerance, and inhibition of salmonella typhimurium infection. our results showed that the membrane composition and morphology of l. johnsonii ncc 533 was significantly changed by supplementing a minimal lactobacillus media with oleic, linoleic and linolenic acids. the ratio of ... | 2011 | 21821758 |
| small-molecular virulence inhibitors show divergent and immunomodulatory effects in infection models of salmonella enterica serovar typhimurium. | the virulence-associated salmonella pathogenicity island 2 (spi2) type iii secretion system supports intracellular replication of salmonella enterica serovar typhimurium in macrophage-like raw264.7 cells. in contrast, the salicylidene acylhydrazide inp0010 and the benzimidazole omeprazole prevent virulence factor-mediated replication of s. typhimurium in these cells. here we show that inp0010 enhances expression of inducible nitric oxide synthase (inos), nitric oxide (no) production, the oxidati ... | 2011 | 21821398 |
| molecular characterisation of multidrug-resistant salmonella enterica serotype infantis from humans, animals and the environment in italy. | during 2005-2006, salmonella enterica serotype infantis strains isolated from human and non-human sources and resistant to ampicillin (a), chloramphenicol (c), streptomycin (s), sulphonamide (su), tetracycline (t), kanamycin (k) and trimethoprim/sulfamethoxazole (sxt) emerged in italy. the aim of this study was to analyse the molecular basis of antibiotic resistance and to evaluate the clonal origin of multiresistant s. infantis strains isolated from different sources. seventy s. infantis strain ... | 2011 | 21821397 |
| secretory nucleoside diphosphate kinases from both intra- and extracellular pathogenic bacteria are functionally indistinguishable. | nucleoside diphosphate kinase (ndk), responsible for the maintenance of ntp pools, is an atp-utilizing enzyme secreted by different pathogens. we found that ndk from salmonella enterica serovar typhimurium (s. typhimurium) is also secretory in nature. secretory ndk is known to play a crucial role in the survival of pathogenic microbes within host cells through their interaction with extracellular atp. to elucidate this aspect, we assessed the contribution of secretory products containing ndk fro ... | 2011 | 21816881 |
| effect of biofilm formation on the excretion of salmonella enterica serovar typhi in feces. | objectives: we hypothesized that salmonella enterica serovar typhi (s. typhi) with higher biofilm and capsule production capability are more able to survive continuously in typhoid patients/carriers, with subsequent prolonged shedding in feces. methods: bacterial cell release from biofilm (produced in vitro and confirmed by specific staining and electron microscopy) and comparative cytotoxicity were studied on caco2 cells. functionality of the biofilm diffusion barrier was tested against ciprofl ... | 2011 | 21816646 |
| prevalence and antimicrobial resistance profiles of salmonella enterica serovars isolated from slaughtered cattle in bahir dar, ethiopia. | a study was undertaken from october 2006 to march 2007 to determine the prevalence and antimicrobial resistance patterns of salmonella serovars. liver, mesenteric lymph nodes, intestinal content, and carcass swab samples (each n = 186) were collected from 186 apparently healthy slaughtered cattle at bahir dar abattoir. bacteriological analysis was done according to the international organization for standardization (iso 6579 2002). isolates were serotyped at agence française de securite sanitair ... | 2011 | 21814750 |
| challenges and opportunities to identifying and controlling the international spread of salmonella. | 2011 | 21813513 | |
| international spread of an epidemic population of salmonella enterica serotype kentucky st198 resistant to ciprofloxacin. | national salmonella surveillance systems from france, england and wales, denmark, and the united states identified the recent emergence of multidrug-resistant isolates of salmonella enterica serotype kentucky displaying high-level resistance to ciprofloxacin. a total of 489 human cases were identified during the period from 2002 (3 cases) to 2008 (174 cases). these isolates belonged to a single clone defined by the multilocus sequence type st198, the xbai-pulsed-field gel electrophoresis cluster ... | 2011 | 21813512 |
| roles of salmonella enterica serovar typhimurium encoded peptidase n during systemic infection of ifnγ(-/-) mice. | pathogen encoded peptidases are known to be important during infection; however, their roles in modulating host responses in immunocompromised individuals are not well studied. the roles of s. typhimurium (wt) encoded peptidase n (pepn), a major aminopeptidase and sole m1 family member, was studied in mice lacking interferon-γ (ifnγ), a cytokine important for immunity. s. typhimurium lacking pepn (δpepn) displays enhanced colony forming units (cfu) compared to wt in peripheral organs during syst ... | 2011 | 21813203 |
| oral administration of a probiotic lactobacillus modulates cytokine production and tlr expression improving the immune response against salmonella enterica serovar typhimurium infection in mice. | abstract: background: diarrheal infections caused by salmonella, are one of the major causes of childhood morbidity and mortality in developing countries. salmonella causes various diseases that range from mild gastroenteritis to enteric fever, depending on the serovar involved, infective dose, species, age and immune status of the host. probiotics are proposed as an attractive alternative possibility in the prevention against this pathogen infection. previously we demonstrated that continuous l ... | 2011 | 21813005 |
| emergence of a globally dominant inchi1 plasmid type associated with multiple drug resistant typhoid. | typhoid fever, caused by salmonella enterica serovar typhi (s. typhi), remains a serious global health concern. since their emergence in the mid-1970s multi-drug resistant (mdr) s. typhi now dominate drug sensitive equivalents in many regions. mdr in s. typhi is almost exclusively conferred by self-transmissible inchi1 plasmids carrying a suite of antimicrobial resistance genes. we identified over 300 single nucleotide polymorphisms (snps) within conserved regions of the inchi1 plasmid, and geno ... | 2011 | 21811646 |
| genetic characterization of conserved charged residues in the bacterial flagellar type iii export protein flha. | for assembly of the bacterial flagellum, most of flagellar proteins are transported to the distal end of the flagellum by the flagellar type iii protein export apparatus powered by proton motive force (pmf) across the cytoplasmic membrane. flha is an integral membrane protein of the export apparatus and is involved in an early stage of the export process along with three soluble proteins, flih, flii, and flij, but the energy coupling mechanism remains unknown. here, we carried out site-directed ... | 2011 | 21811603 |
| extreme antimicrobial peptide and polymyxin b resistance in the genus burkholderia. | cationic antimicrobial peptides and polymyxins are a group of naturally occurring antibiotics that can also possess immunomodulatory activities. they are considered a new source of antibiotics for treating infections by bacteria that are resistant to conventional antibiotics. members of the genus burkholderia, which includes various human pathogens, are inherently resistant to antimicrobial peptides. the resistance is several orders of magnitude higher than that of other gram-negative bacteria s ... | 2011 | 21811491 |
| ancestral genes can control the ability of horizontally acquired loci to confer new traits. | horizontally acquired genes typically function as autonomous units conferring new abilities when introduced into different species. however, we reasoned that proteins preexisting in an organism might constrain the functionality of a horizontally acquired gene product if it operates on an ancestral pathway. here, we determine how the horizontally acquired pmrd gene product activates the ancestral pmra/pmrb two-component system in salmonella enterica but not in the closely related bacterium escher ... | 2011 | 21811415 |
| an allelotyping pcr for identifying <em>salmonella enterica</em> serovars enteritidis, hadar, heidelberg, and typhimurium. | current commercial pcrs tests for identifying salmonella target genes unique to this genus. however, there are two species, six subspecies, and over 2,500 different salmonella serovars, and not all are equal in their significance to public health. for example, finding s. enterica subspecies iiia arizona on a table egg layer farm is insignificant compared to the isolation of s. enterica subspecies i serovar enteritidis, the leading cause of salmonellosis linked to the consumption of table eggs. s ... | 2011 | 21808227 |
| engineering and pre-clinical evaluation of attenuated non-typhoidal salmonella strains serving as live oral vaccines and as reagent strains. | while non-typhoidal salmonella (nts) have long been recognized as a cause of self-limited gastroenteritis, it is becoming increasingly evident that multiple antibiotic-resistant strains are also emerging as important causes of invasive bacteremia and focal infections, resulting in hospitalizations and deaths. we have constructed attenuated salmonella enterica serovar typhimurium and enteritidis strains that can serve as live oral vaccines and as "reagent strains" for subunit vaccine production i ... | 2011 | 21807911 |
| salmonella enteritidis core-o polysaccharide (cops) conjugated to h:g,m flagellin as a candidate vaccine for protection against invasive infection with salmonella enteritidis. | non-typhoidal salmonella serovars enteritidis and typhimurium are a common cause of gastroenteritis but also cause invasive infections and enteric fever in certain hosts (young children in sub-saharan africa, the elderly and immunocompromised). salmonella o polysaccharides (ops) and flagella proteins are virulence factors and protective antigens. the surface polysaccharides of salmonella are poorly immunogenic and do not confer immunologic memory, limitations overcome by covalently attaching the ... | 2011 | 21807909 |
| fliz acts as a repressor of the ydiv gene which encodes an anti-flhd4c2 factor of the flagellar regulon in salmonella enterica serovar typhimurium. | ydiv acts as an anti-flhd(4)c(2) factor, which regulates negatively the class 2 flagellar operons in poor medium in salmonella enterica serovar typhimurium. on the other hand, one of the class 2 flagellar genes fliz encodes a positive regulator of the class 2 operons. in this study, we found that the fliz-dependent activation of the class 2 expression was more profound in poor medium than in rich medium and not observed in the ydiv-mutant background. transcription of the ydiv gene was shown to i ... | 2011 | 21804009 |
| detection of viable cryptosporidium parvum in soil by reverse transcription real time pcr targeting hsp70 mrna. | extraction of high-quality mrna from cryptosporidium parvum is a key step in pcr detection of viable oocysts in environmental samples. current methods for monitoring oocysts are limited to water samples; therefore, the goal of this study was to develop a rapid and sensitive procedure for cryptosporidium detection in soil samples. the effectiveness of five rna extraction methods were compared (mrna extraction with the dynabeads® mrna direct™ kit after chemical and physical sample treatments, and ... | 2011 | 21803904 |
| co-production of acetaldehyde and hydrogen from glucose fermentation by escherichia coli. | escherichia coli k12 strain mg1655 was engineered to co-produce acetaldehyde and hydrogen during glucose fermentation using an exogenous acetyl-coa reductase (for the conversion of acetyl-coa to acetaldehyde) and the native formate hydrogen lyase. a putative acetaldehyde dehydrogenase/acetyl-coa reductase from salmonella enterica (seeute) was cloned, produced at high levels and purified by nickel affinity chromatography. in vitro assays showed that this enzyme had both acetaldehyde dehydrogenase ... | 2011 | 21803884 |
| salmonella carriage in adopted children from mali: 2001-08. | objectives salmonella spp. are among the most frequently encountered bacterial pathogens in children adopted abroad, especially from developing countries. the aim of this study was to investigate the carriage of salmonella in international adoptees over an 84 month period. this screening programme was initiated after serious infections occurred in adopted children. material and methods stool samples taken at the first visit to the outpatient adoption practice and subsequently every month from ch ... | 2011 | 21803770 |
| dynamic modification of microtubule-dependent transport by effector proteins of intracellular salmonella enterica. | intracellular salmonella enterica translocate effector proteins that modify microtubule-dependent transport processes of the host cell and modulate the biogenesis of the salmonella-containing vacuole (scv). one functional consequence is the induction of tubular aggregates of endosomal membranes, termed salmonella-induced filaments or sifs, and further tubular membrane compartments have recently been described. sifs are unique, highly dynamic compartments that form by modification of vesicular tr ... | 2011 | 21803443 |
| [expressing of porcine beta-defensin-2 mature peptide in the yeast]. | to construct the recombinant pichia pastoris km71 in which porcine beta-defensin-2(pbd-2) mature peptide could be stably expressed and the antimicrobial effect was evaluated. | 2011 | 21800634 |
| sensing and adaptation to low ph mediated by inducible amino acid decarboxylases in salmonella. | during the course of infection, salmonella enterica serovar typhimurium must successively survive the harsh acid stress of the stomach and multiply into a mild acidic compartment within macrophages. inducible amino acid decarboxylases are known to promote adaptation to acidic environments. three low ph inducible amino acid decarboxylases were annotated in the genome of s. typhimurium, adia, cada and spef, which are specific for arginine, lysine and ornithine, respectively. in this study, we char ... | 2011 | 21799843 |
| beta-lactam antibiotics prevent salmonella-mediated bovine encephalopathy regardless of the ß-lactam resistance status of the bacteria. | this study assessed the capacity of ß-lactam antibiotics to prevent salmonella-mediated encephalopathy in calves given the putative neuroprotective effects of these drugs of increasing glutamate export from the brain. both ampicillin and ceftiofur prevented the development of encephalopathy despite resistance of the inoculated salmonella enterica serovar saint-paul isolate to both drugs. a glutamate receptor antagonist also prevented this salmonella-mediated encephalopathy. glutamate exporters w ... | 2011 | 21798771 |