Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [development of a qinghaosu-resistant line of plasmodium berghei anka and n strain]. | 1986 | 3296654 | |
| in vitro invasion of host cells by plasmodium berghei sporozoites in serum-free medium. | 1987 | 3295169 | |
| deferoxamine inhibition of malaria is independent of host iron status. | the mechanism whereby deferoxamine (df) inhibits the growth of malaria parasites was studied in rats infected with plasmodium berghei. peak parasitemia was 32.6% (day 14) in untreated controls and 0.15% (day 7) in rats receiving 0.33 mg/g in 8 hourly df injections, subcutaneously. df inhibition of parasite growth was achieved without any reduction in transferrin saturation or hemoglobin synthesis and with only a partial (56%) depletion of hepatic iron stores. dietary iron depletion resulted in a ... | 1988 | 3294334 |
| molecular biology of malaria parasites. | 1988 | 3294023 | |
| the fate of the circumsporozoite antigens during the exoerythrocytic stage of plasmodium berghei. | there has been considerable interest in the circumsporozoite proteins due to their potential use in anti-malarial vaccines. previous authors have shown that these proteins persist from the invading sporozoite throughout the growing exoerythrocytic or liver stage. we show that the different distributions of these proteins seen during the development of the exoerythrocytic parasite of plasmodium berghei closely follow morphological changes, which can be recognized under the light microscope. at th ... | 1988 | 3294008 |
| immunoelectron microscopic localization of circumsporozoite antigen in the differentiating exoerythrocytic trophozoite of plasmodium berghei. | the distribution of the circumsporozoite (cs) antigens in the 24 hour exoerythrocytic trophozoite of p. berghei was studied using lowicryl immunogold electron microscopy. these antigens were present on the plasmalemma of the parasite, in disrupted areas of the host cell cytoplasm adjacent to the trophozoite and around inclusions of the host cell cytoplasm. there was evidence of a redistribution of the cs antigens away from the pellicular region of the sporozoite. | 1988 | 3293804 |
| immune response studies in relation to protection induced by using mdp as an adjuvant in malaria. | muramyl dipeptide (mdp) was an important compound conferring protection to mice against the lethal malaria parasite plasmodium berghei. the mode of protection of this compound was studied using different humoral and cellular parameters. the observations indicate that mdp boosts both humoral antibody response as well as delayed type hypersensitivity reactions, but as far as phagocytosis by macrophages is concerned, malarial mice are already maximally stimulated and mdp makes a marginal difference ... | 1988 | 3292412 |
| malarial parasites induce tnf production by macrophages. | mouse peritoneal macrophages incubated with erythrocytes infected with non-lethal or lethal variants of plasmodium yoelii or with p. berghei, in the presence of polymyxin b to exclude the effects of any contaminating endotoxin, secreted a cytotoxic factor into the supernatant that was shown to be tumour necrosis factor (tnf). no differences were observed in the ability of the three types of parasite to induce tnf production, which was maximal in the range of 0.2-5 infected erythrocytes per macro ... | 1988 | 3292408 |
| stage-selective inhibition of rodent malaria by cyclosporine. | the relative susceptibility of different developmental stages of plasmodium berghei to cyclosporine was investigated in vivo. within 12 h of receiving a single 25-mg/kg (body weight) dose of cyclosporine, mice with patent p. berghei infections uniformly exhibited a rapid fall in asexual parasite stages. initially, ring forms and mature schizonts disappeared. subsequently, trophozoites disappeared between 21 and 24 h, whereas gametocytes persisted for 36 h. in contrast, when cyclosporine was admi ... | 1988 | 3288113 |
| expression of circumsporozoite proteins revealed in situ in the mosquito stages of plasmodium berghei by the lowicryl-immunogold technique. | a monoclonal antibody against the circumsporozoite proteins of the plasmodium berghei sporozoite was used to trace the synthesis and expression of these proteins, via the lowicryl immunogold technique, within the developing oocyst. the proteins were detected on the endoplasmic reticulum of the oocyst and were present in the sporozoite membranes at the point of their formation. | 1988 | 3287283 |
| variation among circumsporozoite protein genes from rodent malarias. | we investigated the effect of long term passage of parasites in naive animals on the circumsporozoite protein (csp) gene of plasmodium yoelii. the csp gene sequence was determined from a non-lethal cloned line of p. yoelii and compared to the csp gene sequence from a lethal strain of p. yoelii. the two parasite lines were originally derived from the same isolate, but were separated 17 years ago followed by continued passage. the sequence of the csp gene and its surroundings from the non-lethal l ... | 1988 | 3287156 |
| plants as sources of antimalarial drugs, part 6: activities of simarouba amara fruits. | extracts prepared from simarouba amara fruits collected in panama have been found to be active against plasmodium falciparum in vitro and against plasmodium berghei in mice. four active quassinoids have been identified as ailanthinone, 2'-acetylglaucarubinone, glaucarubinone and holacanthone. | 1988 | 3287009 |
| protoberberine alkaloids as antimalarials. | the protoberberine alkaloids berberine (1), palmatine (2), jatrorrhizine (3), and several berberine derivatives (4-10) were tested for antimalarial activity in vitro against plasmodium falciparum and in vivo against plasmodium berghei. the berberine derivatives 4-10 were designed and synthesized to maximize structural diversity within a modest set of compounds. palmatine (2) and jatrorrhizine (3) were isolated as their chlorides from enantia chlorantha. none of the protoberberine alkaloids was a ... | 1988 | 3286870 |
| immunosuppression in malaria: effect of hemozoin produced by plasmodium berghei and plasmodium falciparum. | to a considerable degree, malaria-induced immunosuppression has been attributed to an inhibition of macrophage accessory cell function. in this study hemozoin, a plasmodium hemoglobin degradation product which readily accumulates in phagocytic cells and tissues during infection, was examined for its influence on immune responses. hemozoin-laden liver and splenic macrophages from plasmodium berghei-infected mice, displayed accessory cell dysfunction which was likely due to hemozoin loading by the ... | 1988 | 3286520 |
| presence and characterization of lymphocytotoxins in acute and chronic plasmodium berghei malaria. | the role of lymphocytotoxic antibodies in elimination of lymphocytes and characterization of these antibodies during plasmodium berghei infection was examined in balb/c mice. when assayed at 15 degrees, an increase in lymphocytotoxic levels above base line values was detected in acute infection when parasitaemia exceeded 10%. there was an increase in lymphocytotoxins with increase in parasitaemia to a plateau beyond 25% parasitaemia. when assayed at 37 degrees, lymphocytotoxic activity during ac ... | 1988 | 3286484 |
| effect of trans(e)-clopenthixol on plasmodium berghei in vivo. | previous in vitro studies have shown suppression of the growth of plasmodium falciparum by the neuroleptic agents chlorpromazine and zuclopenthixol (formerly known as cis(z)-clopenthixol) as well as by the neuroleptic inactive steroisomer trans(e)-clopenthixol. these compounds are chemically related to riboflavin and may act as inhibitors of riboflavin metabolism. as trans(e)-clopenthixol has been found active against chloroquine-resistant strains of p. falciparum in vitro and has been approved ... | 1988 | 3285868 |
| [electron microscopy study on the action of oocysts and sporozoites of the malaria parasite on the epithelium of the mosquito midgut]. | 1988 | 3285158 | |
| pathologic activity of plasmodium berghei prevented but not reversed by dexamethasone. | dexamethasone has recently been shown to block the production of cachectin (implicated in the pathogenesis of cerebral malaria) if administered prior to endotoxin induction of mouse macrophages. using the hamster cheek pouch-cerebral malaria model, we tested the hypothesis that dexamethasone is effective as a therapeutic agent in severe malaria if given before some yet undefined trigger point in the disease. infected hamsters were treated with dexamethasone (0.7 mg/kg) daily on days 7-12, 4-12, ... | 1988 | 3281490 |
| oral salmonella typhimurium vaccine expressing circumsporozoite protein protects against malaria. | immunization with radiation-attenuated malaria sporozoites induces potent cellular immune responses, but the target antigens are unknown and have not previously been elicited by subunit vaccines prepared from the circumsporozoite (cs) protein. a method is described here for inducing protective cell-mediated immunity to sporozoites by immunization with attenuated salmonella typhimurium transformed with the plasmodium berghei cs gene. these transformants constitutively express cs antigens and, whe ... | 1988 | 3281260 |
| peptide derivatives of primaquine as potential antimalarial agents. | three peptide derivatives of primaquine were synthesized. the compounds were tested for radical curative antimalarial activity against plasmodium cynomolgi in rhesus monkeys and blood schizonticidal antimalarial activity against plasmodium berghei in mice. all three peptide derivatives showed activity against p. cynomolgi greater than that expected for the primaquine content of each prodrug. the toxicity of one of the peptide derivatives was less than that of primaquine in mice. | 1988 | 3280798 |
| 1,2,4-trioxanes as potential antimalarial agents. | a number of 1,2,4-trioxanes were prepared and tested for antimalarial activity in search of a simplified analogue of the naturally occurring antimalarial qinghaosu. the compounds were assayed in an in vitro system for antimalarial activity against chloroquine-susceptible and chloroquine-resistant strains of plasmodium falciparum. the most active compounds were methyl 2-(2,4a-epidioxy-4a,5,6,7,8,8a-hexahydro-5,5,8a-trimethyl-2h-1-benzop yra n-2-yl) acetate (3b), which showed ic20's of 96 and 39 n ... | 1988 | 3280797 |
| [the nitroblue tetrazolium reduction test in evaluating the function of the circulating leukocytes in mice with experimental malaria]. | nbt-test for circulating neutrophils and monocytes in the blood of mice inoculated with plasmodium berghei, strain n or lnk-65, have been performed. within the first 24 h of the infection, before the onset of the recordable parasitemia or in the course of the subsequent six days (depending of the strain used for inoculation) a 50-100% reduction in nbt-positive cells was observed. this demonstrates the ability of malaria parasite to suppress the oxygen-dependent enzyme system in circulating phago ... | 1988 | 3280042 |
| arteether, a new antimalarial drug: synthesis and antimalarial properties. | arteether (6) has been prepared from dihydroquinghaosu (3) by etherification with ethanol in the presence of lewis acid and separated from its chromatographically slower moving alpha-dihydroqinghaosu ethyl ether (7). the absolute stereochemistry at c-12 has been determined by 1h nmr data (j11,12, noesy). ethyl ethers 6 and 7 showed potent in vitro inhibition of plasmodium falciparum, and both compounds were highly potent antimalarials in mice infected with a drug-sensitive strain of plasmodium b ... | 1988 | 3279208 |
| a site of intrinsic bending in a highly repeated element of plasmodium berghei genome. | the basic element of the 2.3 kb repetitive family, present in approximately 300 copies in the plasmodium berghei genome, contains a bent dna region. indications of this given by anomalies in electrophoretic behaviour were confirmed by computational analysis of sequence data. | 1988 | 3278226 |
| acidic phosphoproteins associated with the host erythrocyte membrane of erythrocytes infected with plasmodium berghei and p. chabaudi. | new phosphoproteins appear on the host erythrocyte membrane during plasmodium berghei and p. chabaudi infection. distinct proteins having similar properties and all distinguished by isoelectric points of less than 4.0 are identified. associated with the erythrocyte membranes of p. berghei infected erythrocytes are two proteins with molecular masses of 65 and 46 kda, whereas 93, 90 and 76 kda proteins are observed during p. chabaudi infection. these new erythrocyte membrane associated proteins ar ... | 1988 | 3278220 |
| morphological effects of pyronaridine on malarial parasites. | the ultrastructural changes caused by a new antimalarial drug, pyronaridine, were investigated using mice infected with erythrocytic forms of plasmodium berghei and p. falciparum cultivated in vitro in human erythrocytes. the first changes observed in both parasites after exposure to pyronaridine occurred in the food vacuoles. this suggests that the target organelle of this drug may be the food vacuole of malarial parasites. in addition, rapid alterations were also noted within the pellicular co ... | 1988 | 3277462 |
| effect of cholesterol-rich diet on the susceptibility of rodent malarial parasites to chloroquine chemotherapy. | mice were fed a cholesterol-rich diet and then subsequently infected with chloroquine-sensitive strains of either plasmodium berghei or p. chabaudi. chloroquine therapy, which was started 24 hours post-infection and continued for 3-4 days, was significantly less effective in cholesterol-fed animals compared to controls. the consequences of these findings to the resistance of p. falciparum in man to chloroquine, are discussed. | 1988 | 3275853 |
| detection of all human plasmodium species by a telomeric dna fragment cloned from plasmodium berghei. | a telomeric dna fragment that was cloned from plasmodium berghei was used to detect the genomic dna of p. falciparum, p. vivax, p. malariae, and p. ovale. the fragment hybridized to the dna of all four of these human plasmodium species and can be used as an interspecific probe to detect human malaria. | 1988 | 3266114 |
| malaria infection in rats with stimulated splenic red pulp: the blood flow and protective effect in normal and transplanted splenic tissue. | after splenic autotransplantation both weight and blood flow of the regenerated splenic tissue are decreased. in addition, the protective function of the transplant is less compared to that of the normal spleen. in the present study, the red pulp of normal and transplanted splenic tissue was stimulated by injections of phenylhydrazine to increase the weight, the blood flow, and the protective function. after stimulation, the weight of the normal spleen increased to 900 +/- 70 mg (control 530 +/- ... | 1988 | 3265528 |
| partial cross-reactivity by suppressor cells induced during different experimental autoimmune diseases. | following injection of rat red cells, mice develop anti-red cell autoantibodies and subsequently suppressor t cells specific for these. likewise, following recovery from non-lethal malaria, they develop suppressor t cells which suppress the anti-lymphocyte autoantibodies induced by lethal malaria parasites. neither type of suppressor cell affected non-autoantibody components of the response, nor a response to sheep red cells. however, there was variable but significant cross-suppression of the r ... | 1985 | 3156012 |
| [effects of cycloleucine on the growth and metabolism of malaria parasites]. | 1987 | 3155376 | |
| influence of aflatoxin on malarial infection in mice. | 1988 | 3151410 | |
| plasmodium falciparum: purification, properties, and immunochemical study of ornithine decarboxylase, the key enzyme in polyamine biosynthesis. | ornithine decarboxylase, the rate-limiting enzyme in the polyamine biosynthetic pathway has been purified 7,600 fold from plasmodium falciparum by affinity chromatography on a pyridoxamine phosphate column. the partially purified enzyme was specifically tagged with radioactive dl-alpha-difluoromethylornithine and subjected to polyacrylamide gel electrophoresis under denaturing conditions. a major protein band of 49 kilodalton was obtained while with the purified mouse enzyme, a typical 53 kiloda ... | 1988 | 3139441 |
| sexual development in plasmodium berghei: the use of mitomycin c to separate infective gametocytes in vivo and ookinetes in vitro. | 1987 | 3123410 | |
| reducing effects of rodent malaria on hepatic carcinogenesis induced by dietary aflatoxin b1. | the present study was undertaken to evaluate the effects of plasmodium berghei infection on the development of liver tumors induced in male buffalo rats by aflatoxin b1 (afb1). intraperitoneal (i.p.) injection of 10(6) parasitized red blood cells (prbc) into the rat 12 days prior to administration of 2 ppm dietary afb1 for 10 weeks diminished hepatocellular carcinoma (hcc) induction compared to that observed in rats given afb1 alone at weeks 60-82. no animals in a control group developed hcc les ... | 1988 | 3121525 |
| gamma interferon, cd8+ t cells and antibodies required for immunity to malaria sporozoites. | this study was designed to test the hypothesis that t-cell effector mechanisms are required for protective immunity to malaria sporozoites. administration of neutralizing monoclonal antibodies against gamma interferon (gamma ifn) to immune hosts, reversed sterile immunity to sporozoite challenge, by allowing the growth of exoerythrocytic forms (eef) and thus the development of parasitaemia. immune animals also developed infections when depleted in vivo of their suppressor/cytotoxic t cells expre ... | 1987 | 3120015 |
| the state of ferriprotoporphyrin ix in malaria pigment. | to evaluate the state of ferriprotoporphyrin ix (fp) in malaria pigment, mouse erythrocytes infected with plasmodium berghei nyu-2 parasites were lysed by hypotonic shock, and hemoglobin and other soluble material were removed by extensive washing. the amount of fp recovered in the insoluble pellet was 2.1 mumol/ml of packed infected erythrocytes, of which approximately 1% was attributable to hemoglobin contamination. this crude preparation then was digested with a nonspecific protease from stre ... | 1987 | 3119578 |
| suppression of the chloroquine resistance of plasmodium berghei by treatment of infected mice with a microsomal monooxygenase inhibitor. | administration of a combination of chloroquine and the copper-lysine complex, copper(lysine)(2), an inhibitor of microsomal monooxygenases, considerably decreased the parasitaemia level of mice infected with a chloroquine-resistant strain of plasmodium berghei. when given separately, chloroquine and the complex had no antimalarial effect. use of a combination of monooxygenase inhibitors and chloroquine therefore appears to be a promising addendum to the chemotherapy of malaria caused by chloroqu ... | 1987 | 3117394 |
| chloroquine resistance of plasmodium berghei: biochemical basis and countermeasures. | microsomal monooxygenases, enzymes that metabolize xenobiotics, may be responsible for the chloroquine resistance of malarial parasites. plasmodium cells contain cytochrome p-450 and exhibit aryl hydrocarbon hydroxylase and aminopyrine n-dimethylase activity, two monooxygenases that inactivate chloroquine. the activities of these monooxygenases are considerably higher in chloroquine-resistant strains of plasmodium berghei than in the chloroquine-sensitive strain of the parasite. inhibitors of mi ... | 1987 | 3117393 |
| plasmodium falciparum and plasmodium berghei: effects of ornithine decarboxylase inhibitors on erythrocytic schizogony. | five ornithine decarboxylase inhibitors: alpha-difluoromethylornithine (dfmo) (eflornithine); alpha-monofluoromethyl-3,4-dehydroornithine; alpha-monofluoromethyl-3,4-dehydroornithine methyl ester; alpha-monofluoromethyl-3,4-dehydroornithine ethyl ester; and (2r,5r)-delta-methyl-alpha-acetylenic putrescine were shown to inhibit erythrocytic schizogony of plasmodium falciparum in vitro and reduced spermidine levels in infected erthrocytes. only dfmo was effective at limiting erythrocytic schizogon ... | 1987 | 3115816 |
| [a new molecule in antiparasitic therapy: alpha-difluoromethylornithine]. | alpha-difluoromethylornithine (dfmo) is a specific irreversible inhibitor of ornithine-decarboxylase (odc), key enzyme in the biosynthesis of polyamines, physiological compounds involved in cell multiplication. pharmacokinetic studies of the drug revealed good oral absorption, low metabolisation and mainly urinary excretion. short half-life (3 hrs to 3 hrs 30) implicates daily repeated administrations. dfmo is well tolerated, side effects being reversible on discontinuing drug therapy. they chie ... | 1987 | 3108836 |
| mechanism of escape of exoerythrocytic forms (eef) of malaria parasites from the inhibitory effects of interferon-gamma. | we have studied the mechanism of inhibition by interferon-gamma (ifn-gamma) of the development of exoerythrocytic forms (eef) of plasmodium berghei in the livers of rats. at the time corresponding to the maximum development of eef (44 hr after injection of sporozoites), the livers of the ifn-gamma-treated rats contained less parasite dna as compared with controls. twenty-four to 72 hr later, the livers of both groups of animals were free of parasites; that is, ifn-gamma treatment does not delay ... | 1987 | 3108391 |
| alpha-difluoromethylornithine inhibits the first part of exoerythrocytic schizogony of plasmodium berghei in rodents. | 1986 | 3105475 | |
| [therapeutic trials of experimental murine malaria with the quassinoid, glaucarubinone]. | prevention and treatment of malaria are endangered by the appearance of chemoresistance against the common anti-malarial drugs by plasmodium falciparum. today, only a quinoline derivative, mefloquine, is a safe and effective agent against p. falciparum. an in vitro antiplasmodial activity having been found for the quassinoid glaucarubinone we tested its in vivo therapeutic action on mice infected with a p. berghei strain. at low doses, glaucarubinone retarded mortality by exerting a partial, tem ... | 1987 | 3103880 |
| 2,4-diamino-5-benzylpyrimidines as antibacterial agents. 7. analysis of the effect of 3,5-dialkyl substituent size and shape on binding to four different dihydrofolate reductase enzymes. | a group of trimethoprim (tmp) analogues containing 3,5-dialkyl(or halo)-4-alkoxy, -hydroxy, or -amino substitution were analyzed in terms of their inhibitory activities against four dihydrofolate reductase (dhfr) isozymes. although selectivities were lower than with tmp, the activities against vertebrate dhfr were usually at least 2 orders of magnitude less than against enzymes from microbial sources. however, the profiles of activity were remarkably similar for rat, neisseria gonorrhoeae, and p ... | 1987 | 3100802 |
| alpha-difluoromethylornithine induces protective immunity in mice inoculated with plasmodium berghei sporozoites. | mice inoculated weekly with plasmodium berghei sporozoites while under treatment with alpha-difluoromethylornithine (dfmo), an inhibitor of ornithine decarboxylase, developed protective immunity against subsequent challenge with this parasite. the percentage of mice protected was similar whether dfmo alone (55%) or dfmo + chloroquine (65%) was used. with chloroquine alone, only 12% of mice were protected. this protection was long-lasting (at least six months). the immunity protected against spor ... | 1986 | 3097887 |
| mitomycin-c is an unreliable inhibitor for study of dna synthesis in plasmodium. | cytophotometric studies on dna synthesis during asexual and sexual development of plasmodium berghei contradicted earlier conclusions on dna synthesis in plasmodium which were largely based on experiments in which mitomycin-c had been used as a dna replication inhibitor. therefore, the effect of mitomycin on intra erythrocytic asexual development and on microgametogenesis, fertilization and zygote/ookinete development of p. berghei was studied in vitro. all dna-synthesizing stages (schizonts, ex ... | 1986 | 3095638 |
| enhancing effects of rodent malaria on aflatoxin b1-induced hepatic neoplasia. | the interaction between aflatoxin and malaria was tested for its usefulness as a model for hepatic tumor induction in rats. male buffalo rats which received aflatoxin b1 (afb1) followed by plasmodium berghei infection developed more preneoplastic lesions in the liver compared to those given afb1 alone. no preneoplastic lesions were found in the liver of control and malarial-treated animals. these findings suggest that the malarial parasite facilitates liver tumor development initiated by afb1 in ... | 1986 | 3095331 |
| [suppression of chloroquine resistance in plasmodium berghei by using a microsomal monooxygenase inhibitor]. | 1986 | 3093829 | |
| l3t4+ t lymphocytes play a major role in the pathogenesis of murine cerebral malaria. | the pathogenic importance of l3t4+ t cells in the development of murine cerebral malaria was demonstrated by the following observations. first, in vivo administration of an anti-l3t4 monoclonal antibody protected plasmodium berghei-infected cba mice from the development of neurologic symptoms and acute death. in contrast, injection with an mab directed against the ly.2+ t cell subset had no protective effect. second, thymectomized, irradiated, and bone marrow reconstituted (atxbm) cba mice did n ... | 1986 | 3093572 |
| dna synthesis in plasmodium berghei during asexual and sexual development. | dna contents of individual stages of plasmodium berghei were measured by direct microfluorometry after feulgen-pararosaniline (so2) staining. sporozoites, intra-erythrocytic ringforms and trophozoites (until at least 15 h after invasion) are haploid and non-synthesizing dna. dna is synthesized just before and during schizogony, which takes 4-6 h. genome duplication and segregation are alternating events throughout this process. mature micro- and macrogametocytes have dna contents between the hap ... | 1986 | 3092048 |
| rapid repeated dna replication during microgametogenesis and dna synthesis in young zygotes of plasmodium berghei. | 1986 | 3088783 | |
| inhibition of development of exoerythrocytic forms of malaria parasites by gamma-interferon. | a specific dna probe was used to study the effect of recombinant rat, mouse, and human gamma-interferon (gamma-ifn) on the course of sporozoite-induced malaria infections. in mice and rats infected with sporozoites of plasmodium berghei, mouse and rat gamma-ifn's strongly inhibited the development of the exoerythrocytic forms in the liver liver cells of the hosts, but not the development of the erythrocytic stages. the degree of inhibition of the exoerythrocytic forms was proportional to the dos ... | 1986 | 3085218 |
| 1h and 13c nmr studies of tissue from normal and diseased mice. analysis of t1 and t2 relaxation profiles of triglycerides in liver. | the study was initiated to gain a better understanding of the manifestation of disease in the lipid dynamics in tissue. we have performed high resolution 13c and 1h nmr relaxation measurements on the observable lipid resonances in liver and adipose tissue, excised from mice which were normal fed, normal fasted, and infected with the malarial parasite plasmodium berghei. we have observed that, although the parasite does not invade the hepatocytes, the composition of the liver lipids changes along ... | 1988 | 3079286 |
| ultrastructural studies on morphogenesis of rhoptries in sporozoites of plasmodium berghei (nk 65) in anopheles stephensi. | 1988 | 3077372 | |
| sporogonic stages of p. berghei (nk 65): an ultrastructural study. | 1988 | 3077371 | |
| synthesis and evaluation of 4,6-diamino-1,2-dihydro-2,2'-dialkyl or aryl-1-(substituted)-s-triazines as antimalarial agents. | 1988 | 3077369 | |
| plasmodium berghei: pulmonary oedema and changes in clotting and fibrinolysis during infection in mice. | mice infected with plasmodium berghei, during the ten days of infection, showed significant pulmonary oedema, delayed activated partial thromboplastin time, augmented plasma fibrinolytic activity, oscillations in fibrinogen and unchanged plasminogen levels. these alterations can be expected to cause the release of inflammatory peptides, leading to increased vascular permeability and the generation and/or maintenance of the pronounced pulmonary oedema affecting these animals. | 1988 | 3077022 |
| the chemotherapy of rodent malaria.xliii. indolo (3,2-c) quinoline-n-oxides. | a number of novel indolo (3,2-c) quinoline-n-oxides possess antimalarial activity. one of the series, compound cm 6606, has a superior blood schizontocidal action against drug-sensitive plasmodium berghei in the mouse to that of chloroquine, although it is not fully active against strains highly resistant to chloroquine or mefloquine. it also shows some tissue schizontocidal (causal prophylactic) and gametocytocidal action in mice infected with p. yoelii nigeriensis. the structure activity of th ... | 1988 | 3077021 |
| removal of leucocytes from malaria-infected blood using commercially available filters. | 1988 | 3076749 | |
| [development of plasmodium berghei and the immunosuppression phenomenon in the early phase of experimental malaria in mice]. | 1988 | 3075262 | |
| [modern concepts of the pathogenesis of tropical malaria and trends in the development of a rational therapeutic procedure]. | 1988 | 3075260 | |
| [the antimalarial action of soviet artemisinine in plasmodium berghei schizont infection in mice]. | 1988 | 3075259 | |
| antiparasitic activity of nine pyrazole derivatives against trichomonas vaginalis, entamoeba invadens and plasmodium berghei. | nine nitropyrazole derivatives were prepared and tested against trichomonas vaginalis in vitro and in vivo, entamoeba invadens in vitro and plasmodium berghei in vivo. three of the compounds, 4-4-nitropyrazole, 1-methyl-4-nitropyrazole and 4,4'-dinitro-1,1'-methylenedipyrazole, have an activity similar to that of metronidazole (used as the reference compound) against t. vaginalis and e. invadens after 48 hours of incubation. all the compounds tested were inactive against p. berghei. | 1988 | 3074738 |
| experimental studies on the antimalarial activity estimation by artesunate and artemether preparations per skin absorption. | 1988 | 3073264 | |
| effect of mefloquine and artemisinin on the sporogonic cycle of plasmodium berghei anka in anopheles stephensi mosquitoes. | 1988 | 3072821 | |
| effect of chloroquine on the sporogonic cycle of plasmodium falciparum and plasmodium berghei in anpheline mosquitoes. | 1988 | 3072820 | |
| is malaria involved in the pathogenesis of tropical endomyocardial fibrosis? | 1988 | 3072819 | |
| histamine & kinin system in experimental malaria. | 1988 | 3072298 | |
| [changes in the ability to produce reactive oxygen by phagocytes from mice infected or immunized with plasmodium berghei]. | 1988 | 3072115 | |
| [methods of preselecting febrifugal plants from the madascan flora in view of research on their antimalarial activity]. | 1988 | 3071978 | |
| role of immune complexes in cerebral malaria. | to investigate the involvement of immune complexes in the pathogenesis of cerebral malaria, a comparative study between plasmodium berghei infected swiss albino mice and balb/c mice was conducted. rise in parasitemia was found to be same with differences in manifestation of disease and mortality, level of immune complexes and histopathology. increased parasitized red cells with swelling of endothelial cells in the brain capillaries along with increased levels of circulating immune complexes were ... | 1988 | 3071773 |
| phosphorylation of plasmodium berghei derived phosphoproteins associated with the host erythrocyte membrane by the spectrin kinase. | plasmodium berghei derived phosphoproteins are associated with the host erythrocyte membrane. effectors of the phosphorylation reaction regulate the phosphorylation of the p. berghei derived proteins and spectrin in a similar manner. the spectrin kinase also phosphorylates the p. berghei phosphoproteins in a reconstituted reaction at the same site(s) as the endogenously phosphorylated proteins. these results indicate that a host protein kinase may regulate parasite phosphoproteins during malaria ... | 1988 | 3068518 |
| [determination of the optimal method of treating experimental malaria using artemisinine]. | 1988 | 3068500 | |
| [detection of malarial parasite isoenzymes by cellulose acetate electrophoresis combined with blood concentration in capillaries]. | 1988 | 3068498 | |
| [experimental evidence of the possibility of managing the vector capacity of anopheles mosquitoes]. | 1988 | 3068497 | |
| neutral proteases involved in the reinvasion of erythrocytes by plasmodium merozoites. | neutral proteases of plasmodium sp erythrocytic stages were studied by means of a sensitive fluorogenic method and gelatin-sds-page. the substrates gluconoyl-val-leu-gly-lys(or arg)-3-amido-9-ethylcarbazole were selectively hydrolyzed by an endopeptidase from rodent plasmodium berghei (pb) and plasmodium chabaudi (pc) and from human plasmodium falciparum (pf) parasites. these endopeptidases were purified from 100,000-g soluble schizont extract by high pressure liquid chromatography; they have a ... | 1988 | 3067801 |
| histochemistry of cerebral lesions in mice infected with plasmodium berghei. | the brains of mice with established symptoms of plasmodium berghei erebral malaria were investigated histochemically and histologically. the activity of mitochondrial, lysosomal, glyco/glycogenolytic, hydrolytic and oxidizing enzymes as well as enzymes of the krebs cycle and the pentose cycle was studied during the course of the infection. for comparison cryostate sections were also stained with haematoxylin and eosin, and according to kluver-barrera. changes in enzyme activity, particularly of ... | 1988 | 3067319 |
| liposomes as carriers for a human malaria sporozoite vaccine. | 1988 | 3066667 | |
| immunopathology of thrombocytopenia in experimental malaria. | an early thrombocytopenia was observed in cba mice during acute infection with plasmodium berghei. this was associated with an increase in bone marrow megakaryocytes and a reduction of normal syngeneic 111indium-labelled platelet life span. malaria-induced thrombocytopenia was thus considered to be the result of increased peripheral platelet destruction rather than central hypoproduction. the occurrence of thrombocytopenia was modulated by t-cell depletion. indeed, thymectomized, irradiated or a ... | 1988 | 3065215 |
| [inhibitory effect of artemisinine-sulfadoxine-pyrimethamine combination on the development of drug-resistance in plasmodium berghei]. | 1988 | 3064550 | |
| stage-specific proteins of plasmodium berghei-infected red blood cells detected by antibodies of immune mouse serum. | the asynchronously developing malaria parasite plasmodium berghei was synchronized using in vitro cultivation techniques (mons et al. 1985). after the infection of naive mice, preparations of parasitized erythrocytes with a high level of synchronism could be obtained. immunofluorescence and immunoblotting techniques using serum from immunized mice were applied to determine stage-specific immunogenic molecules in the parasitized erythrocyte preparations. these techniques allowed the detection of ... | 1988 | 3060873 |
| screening for antimalarial activity in the genus potomorphe. | ethanolic extracts (cee) of leaves from potomorphe umbellata and potomorphe peltata, popularly said to have antimalarial capacity, were submitted to the 4-day suppressive test in plasmodium berghei-infected mice. the cee of p. umbellata administered either orally (250 and 1250 mg/kg) or subcutaneously (100 and 500 mg/kg) evidenced strong antimalarial activity, significantly reducing the levels of parasitaemia in a dose-dependent manner. on the other hand, the cee of p. peltata was ineffective in ... | 1988 | 3059067 |
| sex determination and sex differentiation in malaria parasites. | 1988 | 3058219 | |
| [mutagenic effects of n-methyl-n-nitro-n-nitrosoguanidine (mnng) and ethyl methanesulfonate (ems) on chloroquine resistance in plasmodium berghei anka strain]. | 1988 | 3057812 | |
| [development of artemether-resistant of line of plasmodium berghei]. | 1988 | 3057811 | |
| characterization of a specific inhibitor of il2-mediated proliferation from serum of plasmodium berghei infected mice. | serum pools from mice undergoing lethal infection with plasmodium berghei inhibit the growth of an il2-dependent mouse cytotoxic t cell line (ctll). a partially purified preparation of the inhibitory factor specifically inhibited il2-mediated events such as il2-dependent ctll growth and the con a mitogenic response of normal mouse spleen cells. production of and response to il1, as well as growth of myeloma lines, was not affected. administration of the partially purified preparation to normal m ... | 1988 | 3057590 |
| [effect of vitamin e on the lipid composition of the erythrocytes of mice and of the malarial parasite plasmodium berghei]. | 1988 | 3057335 | |
| [effects of large doses of pyronaridine and chloroquine on the ultrastructure of the erythrocytic stages of pyronaridine-resistant line of plasmodium berghei]. | 1988 | 3055816 | |
| malaria sporozoites leave behind trails of circumsporozoite protein during gliding motility. | as plasmodium sporozoites undergo gliding motility in vitro, they leave behind trails of circumsporozoite (cs) protein that correspond to their patterns of movement. this light microscopic observation was made using plasmodium berghei sporozoites, a monoclonal antibody (mab h4) directed against the immunodominant repetitive epitope of the cs protein of p. berghei, and an immunogold-silver staining (igss) technique. sporozoites pretreated with agents that inhibit sporozoite motility and invasiven ... | 1988 | 3054075 |
| the in vitro and in vivo antimalarial activity of some mannich bases derived from 4-[7'-bromo (and chloro)-1',5'-naphthyridin-4'-ylamino]phenol and 4-(7'-trifluoromethylquinolin-4'-ylamino)phenol. | a series of di-mannich bases derived from 4-[7'-bromo (and chloro)-1',5'-naphthyridin-4'-ylamino]phenol and 4-(7'-trifluoromethylquinolin-4'-ylamino)phenol were assayed for activity against chloroquine-sensitive and chloroquine-resistant isolates of cultured plasmodium falciparum using the inhibition of uptake of radiolabelled hypoxanthine. a number of the 4-(7'-trifluoromethylquinolinyl-amino)phenols showed statistically superior activity to chloroquine and amodiaquine against both isolates. an ... | 1988 | 3052332 |
| anemia and anti-erythrocyte antibodies developed after repeated injections of sonicated preparations of plasmodium berghei and babesia rodhaini. | anemia developed in mice after repeated injections of sonicated preparations of plasmodium berghei and babesia rodhaini. anti-erythrocyte antibodies were detected in sera by an enzyme-linked immunosorbent assay and their levels rose as anemia developed. administration of carbon particle suspension to block the mononuclear phagocyte system was effective to inhibit anemia induced by injections of sonicated parasite preparations. sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunob ... | 1988 | 3051800 |
| prevention of experimental cerebral malaria by anticytokine antibodies. interleukin 3 and granulocyte macrophage colony-stimulating factor are intermediates in increased tumor necrosis factor production and macrophage accumulation. | il-3 and granulocyte/macrophage colony stimulating factor (gm-csf) are two cytokines released by activated t lymphocytes that stimulate the growth and differentiation of various hematopoietic cell lines, among which are macrophages. it has been shown that tnf/cachectin, another cytokine that is released mostly by activated macrophages, plays a central role in experimental cerebral malaria (cm), an acute and lethal neurological syndrome induced by plasmodium berghei anka infection in cba mice. si ... | 1988 | 3049913 |
| use of various animal erythrocytes for in vitro cultivation of plasmodium berghei. | 1988 | 3049412 | |
| variation in expression of antibody-dependent cell-mediated cytotoxicity in rodents with malaria. | antibody-dependent cell-mediated cytotoxicity (adcc) against mouse erythrocytes sensitized with immunoglobulin g was studied in mice with malaria. spleen cells from mice had enhanced cytotoxic activity early in plasmodium berghei infection but not later in the disease. sera from infected animals and partially purified malarial immune complexes inhibited adcc. in addition, adcc was diminished in spleen cells from mice infected with the lethal variant of p. yoelii 17x compared with that in mice in ... | 1988 | 3049377 |
| hepatoprotective activity of kutkin--the iridoid glycoside mixture of picrorhiza kurrooa. | 1988 | 3049327 | |
| the antimalarial activity of spathodea campanulata stem bark extract on plasmodium berghei berghei in mice. | 1988 | 3043485 | |
| telomeric motifs are present in a highly repetitive element in the plasmodium berghei genome. | using as probes the subfragments of the telomeric sequence previously cloned by us from plasmodium berghei dna, we identified and cloned a 2.3 kb repeat, largely overlapping the original telomeric insert. restriction mapping indicated that cloned inserts (2.3 kb in length) represented circularly permutated versions of a rather well conserved repeated element, at least in part organized in tandem. the 2.3 kb repeat family with a copy number of about 300 occupies about 4% of the whole genome. the ... | 1987 | 3041211 |
| the production of reactive oxygen species in mice infected or immunized with plasmodium berghei. | the capacity of peritoneal exudate cells (pec) obtained from mice infected or immunized with plasmodium berghei to produce reactive oxygen species (ros) and the biological basis of this response was investigated, using luminol-dependent zymosan-triggered chemiluminescence (cl). cl response of pec from infected mice increased at the early stage but was significantly depressed at the later course of the infection. a similar biphasic activity of peroxidase was also observed in pec from infected mic ... | 1987 | 3037470 |
| toxoplasma gondii and hammondia hammondi: dna comparison using cloned rrna gene probes. | a mung bean nuclease genomic library of purified dna from tachyzoites of the rh strain of toxoplasma gondii was prepared in the bacteriophage lambda gtll and recombinants containing rrna gene fragments were detected by hybridization with radiolabeled total rna from the closely related coccidian eimeria acervulina. ten recombinants were chosen at random, and five of these were investigated further using probes for the genes of the large and small rrna of plasmodium berghei. an insert (called tg4) ... | 1987 | 3034655 |