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bacteriological analysis of burn sepsis.a total of 114 opportunistic bacteria were isolated from 65 swabs from burn sepsis. p. aeruginosa (53.8%) was the most common agent followed by s. aureus (38.4%), klebsiella spp. (27.6%), proteus (18.4%), e. coil (10.7%) and others. the infection was monobacterial in 25 cases (38.4%) and polybacterial in 49 cases (61.5%). p. aeruginosa was predominated in both monobacterial and polybacterial infections. ciprofloxacin (42.9%) was found to be the most effective antibacterial agent. results indicat ...199910695232
evaluation of pigmented intraorifice barriers in endodontically treated teeth.the purpose of this study was to evaluate the effectiveness of three pigmented glass ionomer cements used as intraorifice barriers to prevent coronal microleakage. one hundred ten extracted mandibular human premolars were divided into four experimental groups of 25 teeth each and two control groups of 5 teeth each. the experimental teeth were instrumented and obturated using thermoplasticized gutta-percha and ah26 sealer. group 1 teeth received no further treatment. teeth in groups 2 through 4 h ...199910687533
plant beneficial effect of two strains of proteus vulgaris isolated from tea plantations.two strains of proteus isolated from tea plantation soil were tested for their ability to colonise the roots of gram (cicer arietinum), bean (phaseolus radiatus) and mung (phaseolus mungo) using a gnotobiotic system. seeds bacterized with the two strains grew faster and showed significant increase in root and shoot enlargement of the plants tested. the bioactive fractions obtained from the culture filtrates and separated through hplc showed that the plant growth promoting fractions were not alwa ...199910687288
[the protective activity of anti-proteus blood preparations].human blood anti-proteus preparation activity has been studied on the model of proteus etiology sepsis in white not pedigree mice. it has been found out that anti-proteus plasma and anti-proteus immunoglobulin with antibody titre 1:80 possess marked therapeutic properties. the obtained results of the experimental studies are the grounds for clinical studies carrying out with the use of blood anti-proteus preparations in combined therapy of the patients with diseases of proteus etiology.199910687080
proteus syndrome and immunodeficiency.a 10 year old boy with proteus syndrome presented with a pericardial effusion of unknown aetiology. immunological investigation revealed low serum igg and iga, accompanied by low levels of specific antibodies to pneumococcal and haemophilus type b polysaccharides. circulating lymphocyte surface marker profile revealed t and b cell lymphopenia. this is the first report of hypogammaglobulinaemia occurring in the proteus syndrome.200010685928
biochemical sequence analyses of ges-1, a novel class a extended-spectrum beta-lactamase, and the class 1 integron in52 from klebsiella pneumoniae.klebsiella pneumoniae ori-1 was isolated in 1998 in france from a rectal swab of a 1-month-old girl who was previously hospitalized in cayenne hospital, cayenne, french guiana. this strain harbored a ca. 140-kb nontransferable plasmid, ptk1, that conferred an extended-spectrum cephalosporin resistance profile antagonized by the addition of clavulanic acid, tazobactam, or imipenem. the gene for ges-1 (guiana extended-spectrum beta-lactamase) was cloned, and its protein was expressed in escherichi ...200010681329
microbiologic survey of long-term care facilities.we undertook a microbiologic survey of long-term care facilities to categorize bacteria found in cultures of residents. culture and sensitivity data were collected on 566 samples from indwelling bladder catheters, percutaneous gastrostomy tubes, nares, stool, wounds, pressure ulcers, and tracheostomies in 25 nebraska and iowa facilities. information was also collected on resident factors (eg, presence of indwelling urinary catheter, prior antibiotic administration) and institutional variables (e ...200010679131
antimicrobial chemotherapy in the control of surgical infectious complications.in spite of the progress in surgical technique and antibiotic prophylaxis, postoperative infection still accounts for both the commonest surgical complication and one of the most frequent nosocomial infections, also causing an increase in duration and costs of hospital stay. the choice of treatment for post-surgical infections requires an understanding of the usual infecting flora, available antimicrobial agents, and susceptibility patterns. the most common organisms in simple wound infection ar ...199910678785
investigation of plants used in jamaican folk medicine for anti-bacterial activity.we have started a systematic scientific study of folklore medicinal plants currently used as alternative medicine in jamaican society. in this initial study, extracts of plants widely used by the islanders are studied for antibacterial activity against five common pathogens; streptococcus group a, staphylococcus aureus, proteus mirabilis, pseudomonas aeruginosa and escherichia coli. these studies revealed that 25% (approx.) of the plant extracts had antimicrobial activity against at least one of ...199910678503
proteus, a technical and management model for aquatic risk assessment of industrial spills.the assessment of risks to the aquatic environment related to industrial installations is a priority in environmental pollution control in the netherlands. major accidents to the surface water such as the sandoz incident, but also the high number of smaller accidents that occur every year has invoked the need for an effective method to assess these risks. two different models have been used in this field in the netherlands over several years. these two software applications, veris and risam were ...200010677674
biological activities of lipopolysaccharides of proteus spp. and their interactions with polymyxin b and an 18-kda cationic antimicrobial protein (cap18)-derived peptide.the saccharide constituents of lipopolysaccharides (lps) of proteus spp. vary with the strain and contain unique components about which little is known. the biological activities of lps and lipid a from s- and r-forms of 10 proteus strains were examined. lps from all s-form proteus strains was lethal to d-(+)-galactosamine (galn)-loaded, lps-responsive, c3h/hen mice, but not to lps-hypo-responsive c3h/hej mice. p. vulgaris 025 lps evoked strong anaphylactoid reactions in n-acetylmuramyl-l-alanyl ...200010670563
in vitro evaluation of cefepime and other broad-spectrum beta-lactams for isolates in malaysia and singapore medical centers. the malaysia/singapore antimicrobial resistance study group.the degree of activity of several beta-lactam antimicrobial agents was assessed in malaysia (four medical centers) and singapore (two medical centers) tested against 570 clinical isolates. the organisms were tested locally by the etest (ab biodisk, solna, sweden) method, validated by concurrent use of quality assurance strains (94.1% accurate performance overall). ten groups of bacteria were tested against cefepime, cefpirome, ceftazidime, ceftriaxone, piperacillin/tazobactam, oxacillin, and imi ...199910668586
cloning of l-amino acid deaminase gene from proteus vulgaris.the l-amino acid degrading enzyme gene from proteus vulgaris was cloned and the nucleotide sequence of the enzyme gene was clarified. an open reading frame of 1,413 bp starting at an atg methionine codon was found, which encodes a protein of 471 amino acid residues, the calculated molecular weight of which is 51,518. the amino acid sequence of p. vulgaris was 58.6% identical with the l-amino acid deaminase of p. mirabilis. a significantly conserved sequence was found around the fad-binding seque ...199910664862
antibacterial activity of some folklore medicinal plants used by tribals in western ghats of india.a series of 30 indian folklore medicinal plants used by tribal healers to treat infections, were screened for antibacterial properties at 10 mg/ml concentration by using disc diffusion method against bacillus subtilis, escherichia coli, klebsiella aerogenes, proteus vulgaris, pseudomonas aerogenes and staphylococcus aureus. twenty plant species showed activity against one or more species of bacteria used in this assay; among them the leaf extracts of cassia occidentalis and cassia auriculata exh ...200010661885
encephalocraniocutaneous lipomatosis: a new case report and review of the literature.encephalocraniocutaneous lipomatosis is a rare neurocutaneous syndrome characterized by lipomatous hamartomas ranging in size from a few millimeters to several centimeters and affecting the head. ocular anomalies and a variable degree of mental retardation with or without convulsions are usually observed. this disorder should be distinguished from other mosaic neurocutaneous phenotypes such as proteus syndrome, oculocerebrocutaneous syndrome, and nevus sebaceous syndrome. we report the clinicopa ...199910659502
bacterial resistance to disinfectants: present knowledge and future problems.bacterial resistance to antibiotics is a long-established, widely-studied problem. increasingly, attention is being directed to the responses of various types of microbes to biocides (antiseptics, disinfectants and preservatives). different groups of bacteria vary in their susceptibility to biocides, with bacterial spores being the most resistant, followed by mycobacteria, then gram-negative organisms, with cocci generally being the most sensitive. there are wide divergencies within this general ...199910658759
pcr studies on the presence of chlamydia trachomatis in the upper urinary tract of patients with obstructive pyelonephritis.chlamydia trachomatis infections are among the most common sexually transmitted diseases in the world and it is only logical to hypothesize that it alone or in association with mycoplasmas can participate in the initiation and persistence of upper urinary tract infections. having in mind the inconclusive evidence regarding the role of c. trachomatis in upper urinary tract infections we decided to study the presence of c. trachomatis in the upper urinary tract of patients with obstructive pyelone ...199810658351
[five cases of purple urine bag syndrome in a geriatric ward].we report five cases of purple urine bag syndrome (pubs). all the patients were women and they had been bed-ridden for a long period due to cerebrovascular diseases. they tended to be constipated as a result of habitual use of laxatives. indicanuria was proven in the all urinary samples from the patients. the four assessable urinary cultures showed proteus mirabilis contamination. total days without evacuation per month in patients with pubs and control subjects (5 catheterized subjects without ...199910655742
problems related to determination of mics of oximino-type expanded-spectrum cephems for proteus vulgaris.during in vitro susceptibility testing of clinical isolates of proteus vulgaris, we noted that the mics of several expanded-spectrum cephems were much higher in the broth microdilution method than in the agar dilution method (termed the mic gap phenomenon). here we investigated the mechanism of the mic gap phenomenon. cephems with the mic gap phenomenon were of the oximino type, such as cefotaxime, cefteram, and cefpodoxime, which serve as good substrates for inducible class a beta-lactamase (cu ...200010655366
the ultrastructure of photoreceptor cells in the pineal organ of the blind cave salamander, proteus anguinus (amphibia, urodela).we studied ultrastructure of the photoreceptor cells in the pineal organ of blind, depigmented, neotenic cave salamander, proteus anguinus. unlike in epigean vertebrates the outer segments of most photoreceptor cells consists of concentrically arranged lamellae, however; in few cells, the outer segments contain 7-9 plasma membrane disks. in both types of photoreceptor cells the outer segments enclose lumps of vesicles of different sizes. the photoreceptor cells of proteus anguinus are similar to ...200010653183
functional morphology of the inner ear and underwater audiograms of proteus anguinus (amphibia, urodela).octavolateral sensory organs (auditory and lateral line organs) of cave salamander proteus anguinus are highly differentiated. in the saccular macula of the inner ear the complex pattern of hair cell orientation and the large otoconial mass enable particle displacement direction detection. additionally, the same organ, through air cavities within the body, enables detection of underwater sound pressure changes thus acting as a hearing organ. the cavities in the lungs and mouth of proteus are a r ...200010653179
structure of a 2-aminoethyl phosphate-containing o-specific polysaccharide of proteus penneri 8 from a new serogroup o67.an acidic o-specific polysaccharide was obtained by mild acid degradation of the proteus penneri 8 lipopolysaccharide and found to contain d-glucose, d-galacturonic acid, 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose, 2-acetamido-2,6-dideoxy-l-galactose (l-fucnac) and 2-aminoethyl phosphate (petn) in the ratios 2 : 1 : 1 : 1 : 1 : 1. 1h and 13c nmr spectroscopy was applied to the intact and dephosphorylated polysaccharides, and the following structure of the hexasaccharide repea ...200010651819
structure of the o-specific polysaccharide of proteus penneri 71 and classification of cross-reactive p. penneri strains to a new proposed serogroup o64.a neutral o-specific polysaccharide (o-antigen) was isolated from the lipopolysaccharide (lps) of the bacterium proteus penneri 71. on the basis of sugar analysis and 1h- and 13c-nmr spectroscopic studies, including two-dimensional cosy, 13c,1h heteronuclear cosy and roesy, the following structure of the trisaccharide repeating unit of the polysaccharide was established: -->3)-beta-d-glcpnac-(1-->4)-beta-d-glcpnac-(1-->3)-alpha-d-galp-(1-- > the polysaccharide has the same carbohydrate backbone ...200010651818
structure of a glycerol teichoic acid-like o-specific polysaccharide of proteus vulgaris o12.a phosphorylated o-specific polysaccharide (o-antigen) was obtained by mild acid degradation of proteus vulgaris o12 lipopolysaccharide and studied by sugar and methylation analyses, 1h-, 13c- and 31p-nmr spectroscopy, including two-dimensional cosy, tocsy, noesy, h-detected 1h, 13c and 1h, 31p heteronuclear multiple-quantum coherence experiments. it was found that the polysaccharide consists of pentasaccharide repeating units connected via a glycerol phosphate group, and has the following struc ...200010651815
biosynthetically diverse compounds from a saltwater culture of sponge-derived aspergillus niger.the new compound, asperic acid (1), and the known compounds hexylitaconic acid (2), malformin c (3), pyrophen (4), and asperazine (5) were isolated from the saltwater culture of aspergillus niger derived from a caribbean sponge, hyrtios proteus. the structure elucidation of asperic acid is presented.200010650076
fragmentation of 23s rrna in strains of proteus and providencia results from intervening sequences in the rrn (rrna) genes.intervening sequences (ivss) were originally identified in the rrl genes for 23s rrna (rrl genes, for large ribosomal subunit, part of rrn operon encoding rrna) of salmonella enterica serovars typhimurium lt2 and arizonae. these sequences are transcribed but later removed during rnase iii processing of the rrna, resulting in fragmentation of the 23s species; ivss are uncommon, but have been reported in at least 10 bacterial genera. through pcr amplification of ivs-containing regions of the rrl g ...200010648538
how do intestinal t cells sense the dietary and microbial environment? 200010648476
overgrowth syndromes: an update. 199910645472
[sepsis and antibiotic prophylaxis in stereotaxic neurosurgery].stereotaxic surgery is becoming increasingly important because of the possibility of approaching the deep zones of the brain with less risk. it is in daily use in cerebral tumours and in the functional surgery of parkinson's disease. the use of antibiotic prophylaxis in neurosurgery is controversial, although in many centres, including ours, all patients receive it.199910637862
[weil-felix test]. 199910635793
use of polymerase chain reaction to detect proteus mirabilis and ureaplasma urealyticum in urinary calculi.in this study, we evaluated the efficacy of the polymerase chain reaction (pcr) in detecting urea-splitting microorganisms in desiccated urinary tract infection stones. seventy-eight urinary tract stones were tested for the presence of proteus mirabilis and ureaplasma urealyticum by means of pcr with species-specific primers. twenty-seven stone samples were composed of struvite and/or carbonate apatite (infection stone); 40 were calcium oxalate and/or calcium phosphate; seven were mixed, with st ...199910634025
rapid turnover of flhd and flhc, the flagellar regulon transcriptional activator proteins, during proteus swarming.the enterobacterial flhdc master operon activates expression of the flagellar biogenesis gene hierarchy and also represses cell division. during proteus mirabilis differentiation into elongated hyperflagellated swarm cells, flhdc transcription is strongly but transiently increased. we show that concentration of the flhd and flhc proteins is also tightly controlled at the posttranslational level. this is achieved by protein degradation, which is most severe after differentiation when the half-lif ...200010633123
structure of a 2-aminoethyl phosphate-containing o-specific polysaccharide of proteus penneri 63 from a new serogroup o68.lipopolysaccharide of proteus penneri strain 63 was degraded by mild acid to give a high molecular mass o-specific polysaccharide that was isolated by gel-permeation chromatography. sugar and methylation analyses and nmr spectroscopic studies, including two-dimensional 1h, 1h cosy, tocsy rotating-frame noe spectroscopy, h-detected 1h,13c and 1h,31p heteronuclear multiple-quantum coherence (hmqc), and 1h, 13c hmqc-tocsy experiments, demonstrated the following structure of the polysaccharide: wher ...200010632731
intestinal protozoa in hiv-infected patients: effect of rifaximin in cryptosporidium parvum and blastocystis hominis infections.in hiv-1 infected patients severe enteritis and chronic diarrhea are often documented as a consequence of multiple opportunistic infections. we analyzed 48 hiv-1 positive patients for the presence of intestinal pathogenic protozoa. patients with cd4 > or = 200/mm3 showed a higher prevalence of a single pathogenic protozoa than patients with cd4 < or =200/mm3, who showed the presence of multiple protozoal infections. patients who proved positive for only a single protozoa, cryptosporidium or blas ...199910632386
sub-mic ciprofloxacin effect on fimbrial production by uropathogenic escherichia coli strains.the urine from 210 patients with acute urinary tract infection (uti) was examined to study the in vitro effect of ciprofloxacin on fimbriae production by uropathogenic escherichia coli isolates. forty-nine bacterial samples of density 10(5) cfu/ml were not considered. from the resulting 161 samples, e. coli was the major strain found, present in 54 samples. other microoganisms found were: enterococcus sp. (34 samples), staphylococcus epidermis (22), yeasts (11), proteus sp. (11), pseudomonas sp. ...199910632381
[the results of a multicenter study of the comparative activity of cefepime and other antibiotics against the causative agents of severe hospital infections (the micromax program)].a multicentre trial was performed on the activity of cefepime in comparison with ceftazidime, ceftriaxone, piperacillin/tazobactam, imipenem and ciprofloxacin against severe hospital infection pathogens in intensive care units. the isolates of escherichia coli and proteus spp. from the majority of the centres were highly susceptible to the antibiotics (90 to 100 per cent of the isolates). in some centres up to 40 per cent of the isolates produced esbl. the isolates of klebsiella spp. were charac ...199910629736
role and mechanism of action of c. pvuii, a regulatory protein conserved among restriction-modification systems.the pvuii restriction-modification system is a type ii system, which means that its restriction endonuclease and modification methyltransferase are independently active proteins. the pvuii system is carried on a plasmid, and its movement into a new host cell is expected to be followed initially by expression of the methyltransferase gene alone so that the new host's dna is protected before endonuclease activity appears. previous studies have identified a regulatory gene (pvuiic) between the dive ...200010629196
dynamic aspects of the structured cell population in a swarming colony of proteus mirabilis.proteus mirabilis forms a concentric-ring colony by undergoing periodic swarming. a colony in the process of such synchronized expansion was examined for its internal population structure. in alternating phases, i.e., swarming (active migration) and consolidation (growth without colony perimeter expansion), phase-specific distribution of cells differing in length, in situ mobility, and migration ability on an agar medium were recognized. in the consolidation phase, the distribution of mobile cel ...200010629184
[surgical policy in false aneurysm of proximal anastomosis secondary to reconstruction of aortofemoral segment].the results of surgical treatment of 10 patients with a false aneurysm of the proximal anastomosis are analyzed. an average time for development of a false aneurysm made up 57.2 months. fluorlon-lavsan vascular woven prosthesis of russian produce was used in primary reconstruction. bacteriological examination of operation material was carried out in 9 patients. 8 cases revealed the growth: of staph. aureus (4) epidermal staphylococcus flora (3) and proteus vulg. (1). the authors suggest that sur ...199910626379
disk diffusion susceptibility test development for the new glycylcycline, gar-936.the in vitro activity of gar-936, a new semisynthetic glycylcycline, was evaluated in comparison with two tetracyclines and the disk diffusion susceptibility test was assessed. nearly 700 recent clinical isolates were tested by reference broth microdilution and disk diffusion (two disk contents) methods. among the enterobacteriaceae, gar-936 was generally two- to 16-fold more active than other tetracyclines. all enteric bacilli mic90 results were < or = 4 micrograms/ml; the exception being prote ...199910626138
insertional transposon mutagenesis by electroporation of released tn5 transposition complexes.dna transposition is an important biological phenomenon that mediates genome rearrangements, inheritance of antibiotic resistance determinants, and integration of retroviral dna. transposition has also become a powerful tool in genetic analysis, with applications in creating insertional knockout mutations, generating gene-operon fusions to reporter functions, providing physical or genetic landmarks for the cloning of adjacent dnas, and locating primer binding sites for dna sequence analysis. dna ...200010625401
aortic ring abscess and aortoatrial fistula complicating fulminant prosthetic valve endocarditis due to proteus mirabilis. 200010625192
stimulatory effects of endotoxin on the platelet secretory process.the effects of the lipopolysaccharides (lps) of proteus mirabilis (smooth and rough types), differing significantly in their composition on the release of compounds stored in specific platelet granules, were studied. there are two main types of secretory granules in blood platelets. dense granules contain adenine nucleotides, and in alpha-granules different proteins are stored. the lps were found to cause a dose-dependent release of proteins and adenine nucleotides. in the extracellular medium l ...199910624010
interactions of "limax amoebae" and gram-negative bacteria: experimental studies and review of current problems.free-living amoebae can harbour bacteria inside their cysts giving them a microhabitat and protecting them from disinfectants. the aim of this study was to evaluate the potential importance of "limax amoebae" as vectors for environmental and nosocomial bacteria in a hospital. it was shown that free-living amoebae are ubiquitous in the investigated hospital, occur syntopically with facultative human pathogens (comamonas acidovorans and pseudomonas aeruginosa) and may serve as hosts not only for t ...199810622622
synthesis of cefminox by cell-free extracts of streptomyces clavuligerus.in vitro synthesis of cefminox by cell-free extracts of streptomyces clavuligerus was investigated using alpha-ketoglutarate, l-ascorbic acid, feso(4), s-adenosyl-l-methionine, and 7alpha-demethoxycefminox as the substrates. the formation of cefminox was detected both by a biological assay with proteus vulgaris gn 76/c-1 and by high performance liquid chromatography. although the conversion rate of 7alpha-demethoxycefminox to cefminox was observed to be quite low, it still demonstrated the poten ...200010620685
introduction of a mini-gene encoding a five-amino acid peptide confers erythromycin resistance on bacillus subtilis and provides temporary erythromycin protection in proteus mirabilis.a 15-bp mini-gene was introduced into bacillus subtilis and into stable protoplast-like l-forms of proteus mirabilis. this mini-gene encoded the peptide mvlfv and modeled a fragment of escherichia coli 23s rrna responsible for e. coli erythromycin (ery) resistance. expression of the introduced mini-gene conferred permanent ery resistance on b. subtilis. in l-forms of p. mirabilis, the ery-protective effect was maintained in the course of several generations. herewith, the mechanism of ery resist ...200010620668
isolation and antibacterial activity of phenylpropanoid derivatives from ballota nigra.in addition to the previously isolated phenylpropanoid glycosides verbascoside 1, forsythoside b 2, arenarioside 3 and ballotetroside 4, another four compounds were isolated from generative aerial parts of ballota nigra: three phenylpropanoid glycosides, alyssonoside 5, lavandulifolioside 6 and angoroside a 7 and a non-glycosidic derivative (+)-(e)-caffeoyl-l-malic acid 8. the antibacterial activity of the five major compounds (1-4 and 8) was tested against gram-positive and gram-negative bacter ...199910619384
immediate and long-term galvanotactic responses of amoeba proteus to dc electric fields.the long-term and immediate galvanotactic responses of amoeba proteus to the direct current electric fields (dcefs) were studied with the methods of computer-aided image analysis. it was found that in contrast to earlier reports, amoebae continued locomotion towards cathode (the negative pole) for hours and the increase in the field strength in the range 300-600 mv/mm caused the straightening of cell trajectories accompanied by the decreased frequency of the lateral pseudopods formation and less ...200010618163
convenient test for screening metallo-beta-lactamase-producing gram-negative bacteria by using thiol compounds.a simple disk diffusion test was constructed for detection of imp-1-type metallo-beta-lactamase-producing gram-negative bacteria. two kirby-bauer disks containing ceftazidime (caz) and a filter disk containing a metallo-beta-lactamase inhibitor were used in this test. several imp-1 inhibitors such as thiol compounds including 2-mercaptopropionic acid, heavy metal salts, and edta were evaluated for this test. two caz disks were placed on a mueller-hinton agar plate on which a bacterial suspension ...200010618060
comparative evaluation of five chromogenic media for detection, enumeration and identification of urinary tract pathogens.five chromogenic agar plates--cps id2 medium (biomérieux, france), chromagar orientation medium (becton dickinson, france), uriselect3 medium (sanofi diagnostics pasteur, france), rainbow agar uti medium (biolog, usa) and chromogenic uti medium (oxoid, germany)--for the detection, enumeration and direct identification of urinary tract pathogens were compared using 443 urine specimens at two hospital laboratories. the enumeration of microorganisms was consistent on the five media for 403 of the 4 ...199910614954
[erythrocytic glucose-6-phosphate dehydrogenase deficiency, hemolysis and diabetic ketoacidosis]. 199910603677
proteus syndrome: craniofacial and cerebral mri.the proteus syndrome is a rare hamartoneoplastic syndrome that may affect the brain, skull, and extracranial head and neck. we present a case with severe, characteristic findings. brain abnormalities are not common in proteus syndrome; when present, hemimegalencephaly and migrational disorders are typically seen, commonly with an associated seizure disorder. maxillary and mandibular dysmorphism may occur, including unilateral condylar hyperplasia. subcutaneous fatty, fibrous, lymphangiomatous ma ...199910602859
production of a tem-24 plasmid-mediated extended-spectrum beta-lactamase by a clinical isolate of pseudomonas aeruginosa.several pseudomonas aeruginosa strains, including one urinary isolate producing an extended-spectrum beta-lactamase tem-24, were isolated from a long-term-hospitalized woman. three tem-24-producing enterobacterial species (enterobacter aerogenes, escherichia coli, and proteus mirabilis) were isolated from the same patient. tem-24 and the resistance markers for aminoglycosides, chloramphenicol, and sulfonamide were encoded by a 180-kb plasmid transferred by conjugation into e. coli hb101.200010602754
the structure of the carbohydrate backbone of core-lipid a region ofthe lipopolysaccharides from proteus mirabilis wild-type strain s1959 (serotype o3) and its ra mutant r110/1959.the following structure of core-lipid a region of the lipopolysaccharide (lps) from proteus mirabilis strain 1959 (serotype o3) and its rough mutant r110/1959 (proteus type ii core) was determined using nmr and chemical analysis of the core oligosaccharide, obtained by mild acid hydrolysis of lps, and of the products of alkaline deacylation of the lps: incomplete substitutions are indicated by italics. all sugars are in pyranose form, alpha-hep is the residue lglycero-alpha-dmanno-hep, alpha-dd- ...200010601875
gram-negative bacillary meningitis in adult post-neurosurgical patients.to assess the clinical features and therapeutic outcomes of gram-negative bacillary meningitis (gnbm) in adult postneurosurgical patients.199910595761
nephrobronchial fistula secondary to xantogranulomatous pyelonephritis.we report a case of staghorn nephrolithiasis that evolved into xanthogranulomatous pyelonephritis with perinephric abscess, nephrobronchial fistula, and lung abscess. the patient was an intravenous drug abuser who tested positive for human immunodeficiency virus, without evidence of acquired immunodeficiency syndrome. he presented with a 2-month history of untreated repeated episodes of left flank pain and hyperpyrexia. treatment involved left nephrectomy, debridement of abscess, tube drainage, ...199910585069
molecular recognition of synthetic siderophore analogues: a study with receptor-deficient and fhu(a-b) deletion mutants of escherichia coli.the biological activity of six synthetic siderophore analogues (two dihydroxamates, two trihydroxamates, one tetrahydroxamate and one 3-hydroxy-4(1h)pyridinone) has been studied in escherichia coli, morganella morganii 13 and proteus mirabilis 8993 strains by using growth promotion tests. various transport-deficient mutants of e. coli were used to study the route of entry into gram-negative bacteria. the results indicated that the synthetic hydroxamate compounds are transported via fhu-mediated ...199910581683
evaluation of the in vitro antimicrobial activity of cefepime compared to other broad-spectrum beta-lactams tested against recent clinical isolates from 10 chinese hospitals. chinese antimicrobial resistance study group.a surveillance study was initiated in china in 1998 in which 10 medical centers participated. the susceptibility profiles of 996 commonly occurring pathogens belonging to 10 different species groups were tested by the etest (ab biodisk, solna, sweden) against six broad-spectrum beta-lactam antimicrobial agents (cefepime, ceftazidime, ceftriaxone, imipenem, cefoperazone/sulbactam and piperacillin or oxacillin). quality control was closely monitored and cefepime- and/or imipenem-resistant enteroba ...199910579094
cartilage degradation by hyaluronate lyase and chondroitin abc lyase: a maldi-tof mass spectrometric study.matrix-assisted laser desorption ionization and time-of-flight mass spectrometry (maldi-tof ms) has been used to investigate degradation products of two selected polysaccharides of cartilage (chondroitin sulfate and hyaluronic acid). testicular hyaluronate lyase and chondroitin abc lyase were used for enzymic digestion of both polysaccharides as well as of cartilage specimens. polysaccharide solutions and cartilage supernatants were assayed by positive and negative maldi-tof ms. especially chond ...199910576924
[advances and prospects of a combined therapy of acute radiation injury in experiments].we present the results of a long-standing experimental development of ways and means for acute radiation sickness treatment that have been authorized for application in medicine and are mostly aimed at large-scale accidental injuries. the paper describes means for early treatment (prodigiosan, desoxynate, typhoid vaccine, proteus vaccine), a myeolopoiesis stimulant (estradiol dipropionate), a detoxication procedure (hemosorption), substitution therapy with bone marrow cells and peripheral blood ...199910576028
the structure of the core part of proteus vulgaris ox2 lipopolysaccharide.the identity of a novel structural component, an open-chain acetalic linkage, in the core part of the lipopolysaccharide (lps) from proteus vulgaris serotype ox2 has been determined by extensive nmr spectroscopic analysis of fragments isolated after mild acid hydrolysis of the intact lps. the open-chain n-acetylgalactosamine fragment is substituted in the 4-position by non-stoichiometric amounts of a beta-galactopyranose residue and the overall structure of the core is as follows: [formula: see ...199910573861
serum immunoglobulin response and protection from homologous challenge by proteus mirabilis in a mouse model of ascending urinary tract infection.we tested the hypothesis that experimental proteus mirabilis urinary tract infection in mice would protect against homologous bladder rechallenge. despite production of serum immunoglobulin g (igg) and igm (median titers of 1:320 and 1:80, respectively), vaccinated (infected and antibiotic-cured) mice did not show a decrease in mortality upon rechallenge; the survivors experienced only modest protection from infection (mean log(10) number of cfu of p. mirabilis nal(r) hi4320 per milliliter or gr ...199910569791
effect of thermal injury with pseudomonas aeruginosa infection on pulmonary and systemic bacterial clearance.despite improvements in burn wound care, infections, particularly pneumonia, remain a major hurdle to recovery from thermal injury. after burns, a variety of systemic immune and inflammatory changes contribute to the risk of infection. clinically, infection coupled with burn injury seems to adversely affect susceptibility to subsequent infection.199910568721
preseptal cellulitis secondary to proteus species: a case report and review.preseptal cellulitis is a serious ocular condition that--if left untreated--has the potential to cross the septal barrier, spread to the posterior orbit, and may result in fatal complications. because it is difficult to determine the pathogen responsible for any cellulitis without aspirating a culture sample, treatment is usually instituted by an assumption of the most common causative organisms, staphylococcus or streptococcus.199910561925
clostridium difficile toxins may augment bacterial penetration of intestinal epithelium.clostridium difficile can be recovered from many high-risk hospitalized patients receiving broad-spectrum antibiotic therapy. clostridium difficile toxins a and b have been associated with increased intestinal permeability in vitro and there is growing evidence that increased intestinal permeability may be a common mechanism whereby enteric bacteria penetrate the intestinal epithelium.199910555639
the phylogenetic position of serratia, buttiauxella and some other genera of the family enterobacteriaceae.the phylogenetic relationships of the type strains of 38 species from 15 genera of the family enterobacteriaceae were investigated by comparative 16s rdna analysis. several sequences of strains from the genera citrobacter, erwinia, pantoea, proteus, rahnella and serratia, analysed in this study, have been analysed previously. however, as the sequences of this study differ slightly from the published ones, they were included in the analysis. of the 23 enterobacterial genera included in an overvie ...199910555323
minimal debridement in the treatment of fournier's gangrene.scrotal and perineal gangrene of the male genitalia was first described by fournier in 1883. treatment of the disease remains surgical despite the availability of modern antibiotics. many authors advocate a wide debridement of all tissue superficial to the involved fascial layers. we have reviewed our experience of using wide and minimal debridement as the surgical technique of choice. in our retrospective study, nine patients were diagnosed and treated over a 2-year period for fournier's gangre ...199910551751
antimicrobial activities of several parts of pinus brutia, juniperus oxycedrus, abies cilicia, cedrus libani and pinus nigra.in this study, the antimicrobial activities of several parts of various trees grown in the kahramanmaraş region of turkey were investigated by the disc diffusion method. chloroform, acetone and methanol extracts of leaves, resins, barks, cones and fruits of pinus brutia ten., juniperus oxycedrus l., abies cilicia ant. & kotschy carr., cedrus libani a. rich. and pinus nigra arn. were prepared and tested against bacillus megaterium dsm 32, bacillus subtilis img 22, bacillus cereus fmc 19, escheric ...199910548751
diversity of tem mutants in proteus mirabilis.in a survey of resistance to amoxicillin among clinical isolates of proteus mirabilis, 10 tem-type beta-lactamases were characterized: (i) the well-known penicillinases tem-1 and tem-2, the extended-spectrum beta-lactamases (esbls) tem-3 and tem-24, and the inhibitor-resistant tem (irt) tem-44 and (ii) five novel enzymes, a penicillinase tem-57 similar to tem-1, an esbl tem-66 similar to tem-3, and three irts, tem-65, tem-73, and tem-74. the penicillinase tem-57 and the esbl tem-66 differed from ...199910543745
an exploration of the accuracy of an invasive method for measuring the volume of specimens of amoeba proteus.a method is described for measuring the volume of individual specimens of amoeba proteus which utilizes an easily constructed compressor to flatten the specimen to a known thickness. the microscopic image of the flattened specimen is captured on tape, digitized and analysed with the nih image software. the results from one specimen are given to illustrate the sources and magnitude of errors affecting these volume measurements.199910540260
a child with hemimegalencephaly, hemihypertrophy, macrocephaly, cutaneous vascular malformation, psychomotor retardation and intestinal lymphangiectasia--a diagnostic dilemma.although the clinical delineation of the majority of overgrowth syndromes is straightforward, we believe there is a subset of patients with overlapping features from a number of overgrowth syndromes. we report a patient with hemimegalencephaly, hemihypertrophy, macrocephaly, vascular lesions, psychomotor retardation and intestinal lymphangiectasia. the clinical history and findings posed a diagnostic dilemma as the features overlapped between several conditions, namely macrocephaly-cutis marmora ...199910532178
identification and molecular analysis of the gene encoding rickettsia typhi hemolysin.rickettsia typhi, the causative agent of murine typhus, grows directly within the host cell cytoplasm, accumulating a large number of progeny, and eventually lyses the cells. typhus group rickettsiae (r. typhi and r. prowazekii) adhere to and lyse human and sheep erythrocytes. however, the molecular mechanism underlying erythrocyte lysis by r. typhi has not been defined. here we describe the cloning and nucleotide sequence analysis of the gene (tlyc) encoding a hemolysin from r. typhi. dna seque ...199910531273
characteristics of pathogens causing urinary tract infections in hospitals in north america: results from the sentry antimicrobial surveillance program, 1997.urinary tract infection (uti) is common and involves pathogens with changing susceptibility patterns. the sentry antimicrobial surveillance program evaluates international pathogen incidence patterns to detect and manage the emergence of resistant strains. we describe the antimicrobial resistance patterns among 1617 pathogens recovered from utis during the third-quarter of 1997 in north america (united states and canada), as part of this worldwide program. the isolates were tested against more t ...199910529882
proteus mirabilis viability after lithotripsy of struvite calculi.we tested the hypotheses that proteus mirabilis viability of struvite calculi differs after exposure to different lithotripsy modalities and that the photothermal mechanism of holmium:yag lithotripsy is antibacterial.199910524893
evolutionary changes reflected by the cellular amino acid composition.comparison of the amino acid composition of cell-proteins using 17 amino acids has been used to investigate the biological evolution of organisms such as bacteria, blue-green alga, green alga, fungi, slime mold, protozoa and vertebrates. the degree of difference in the amino acid ratios between any two groups reflects the degree of divergency in biological evolution. the amino acid composition of the gram-negative bacteria (escherichia coli, klebsiella, proteus, and vibrio alginolyticus) was ide ...199910524278
molecular mechanisms of interaction of rabbit cap18 with outer membranes of gram-negative bacteria.the mechanism of interaction of the cationic antimicrobial protein (18 kda), cap18, with the outer membrane of gram-negative bacteria was investigated applying transmission electron microscopy and voltage-clamp techniques on artificial planar bilayer membranes. electron micrographs of bacterial cells exposed to cap18 showed damage to the outer membrane of the sensitive escherichia coli strains f515 and atcc 11775, whereas the membrane of the resistant proteus mirabilis strain r45 remained intact ...199910521271
structure of an acidic o-specific polysaccharide of proteus mirabilis o5.the following structure of the o-specific polysaccharide of proteus mirabilis o5 was established by 1h and 13c nmr spectroscopy at 500 mhz, including two-dimensional cosy, tocsy, noesy, and h-detected 1h, 13c heteronuclear multiple-quantum coherence (hmqc) experiments: [formula: see text] where o-acetylation of alpha-d-glcnac at both positions is nonstoichiometric.199910520266
the structure of the core part of proteus mirabilis o27 lipopolysaccharide with a new type of glycosidic linkage.the structural assignment of the intact lipopolysaccharide core from proteus mirabilis o27 has been completed based on a combination of chemical degradation studies, nmr spectroscopy and es-ms spectroscopy. the overall core structure is as follows: [formula: see text] where all sugars are in the pyranose form except the n-acetylglycosamine residue, hep refers to l-glycero-alpha-d-manno-heptopyranose and alpha-ddhep to d-glycero-alpha-d-manno-heptopyranose. bold italics indicate non-stoichiometri ...199910520257
the use of chromagar orientation as a primary isolation medium with presumptive identification for the routine screening of urine specimens.the aim of the study was to compare the use of a novel differential culture medium chromagar, for both primary isolation and presumptive identificaton, with the method currently used in our laboratory for screening mid-stream-urine samples (msu). routine methods (rm) included blotting paper imprinting of all specimens and additional quantitative culture on cysteine lactose electrolyte-deficient agar (cled) for selected samples together with microbact 12e for further identification. the chromagar ...199910519322
abolition of swarming of proteus. 199910515741
structure and cross-reactivity of the o-antigen of proteus vulgaris o8.a high-molecular-mass o-specific polysaccharide was obtained by mild acid degradation of proteus vulgaris o8 lipopolysaccharide followed by gel permeation chromatography. studies of the polysaccharide by sugar and methylation analyses and 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, and h-detected 1h, 13c heteronuclear multiple-quantum coherence (hmqc) experiments, demonstrated the presence of a tetrasaccharide repeating unit having the following structure: [sequence: see text] ...199910515057
structure of a new acidic o-antigen of proteus vulgaris o22 containing o-acetylated 3-acetamido-3,6-dideoxy-d-glucose.the acidic o-specific polysaccharide of proteus vulgaris o22 was studied using 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, and h-detected 1h, 13c heteronuclear multiple-quantum coherence (hmqc) experiments, and the following structure for the branched pentasaccharide repeating unit was established: [sequence: see text] where qui3nac is 3-acetamido-3,6-dideoxyglucose, o-acetylation of quinac at position 4 is stoichiometric and at position 2 nonstoichiometric. serological relatio ...199910515053
in vitro antibacterial activity of fk041, a new orally active cephalosporin.the in vitro activity of fk041, a new orally active cephem antibiotic, against a wide variety of clinical isolates of bacteria was investigated and compared with those of cefdinir (cfdn) and cefditoren (cdtr). fk041 exhibited broad spectrum activity against reference strains of gram-positive and gram-negative aerobes and anaerobes. fk041 was active against clinical isolates of gram-positive organisms except enterococcus faecalis with mic90s less than 1.56 microg/ml. fk041 was more active than cf ...199910513845
association of rickettsia helvetica with chronic perimyocarditis in sudden cardiac death.rickettsia helvetica is the only non-imported rickettsia found in scandinavia. it was first detected in ixodes ricinus ticks, but has never been linked to human disease. we studied two young swedish men who died of sudden cardiac failure during exercise, and who showed signs of perimyocarditis similar to those described in rickettsial disease.199910513711
antibiotic sensitivity of bacteria associated with community-acquired urinary tract infection in britain.twelve laboratories in different parts of britain each supplied approximately 80 consecutive urinary bacterial isolates from community patients. all strains were identified by a central laboratory, where sensitivity to a variety of orally administered antimicrobials was determined by microtitre broth dilution. 65.1% of isolates were escherichia coli, 23.4% 'coliforms' other than e. coli, 4.6% proteus and morganella spp., 1.8% pseudomonas spp., 2.4% enterococci, 0.7% group b streptococci, 1.5% co ...199910511403
quorum-sensing cross talk: isolation and chemical characterization of cyclic dipeptides from pseudomonas aeruginosa and other gram-negative bacteria.in cell-free pseudomonas aeruginosa culture supernatants, we identified two compounds capable of activating an n-acylhomoserine lactone (ahl) biosensor. mass spectrometry and nmr spectroscopy revealed that these compounds were not ahls but the diketopiperazines (dkps), cyclo(deltaala-l-val) and cyclo(l-pro-l-tyr) respectively. these compounds were also found in cell-free supernatants from proteus mirabilis, citrobacter freundii and enterobacter agglomerans [cyclo(deltaala-l-val) only]. although ...199910510239
genomic subtractive hybridization and selective capture of transcribed sequences identify a novel salmonella typhimurium fimbrial operon and putative transcriptional regulator that are absent from the salmonella typhi genome.salmonella typhi, the etiologic agent of typhoid fever, is adapted to the human host and unable to infect nonprimate species. the genetic basis for host specificity in s. typhi is unknown. the avirulence of s. typhi in animal hosts may result from a lack of genes present in the broad-host-range pathogen salmonella typhimurium. genomic subtractive hybridization was successfully employed to isolate s. typhimurium genomic sequences which are absent from the s. typhi genome. these genomic subtracted ...199910496884
[clinical success of treatment of chronic otitis media using topical and peroral administration of ofloxacin].the paper presents results of the treatment of 32 patients (35 ears) with chronic otitis media and long lasting purulent otorrhea with local and oral ofloxacin. the patients were divided in two groups. group a included 17 patients (49%), with previous middle ear surgery. group b comprised 15 patients (18 ears, 51%) treated with local and systemic antimicrobial drugs. ofloxacin was administered after taking the swab sample, and the duration of treatment was the same in all patients. the most freq ...199910494153
[development of a vaccine for treatment of urinary tract infections].receiving uroinfections are predominantly treated with antibiotics and chemotherapeutics. an effective alternative treatment can be immunotherapy with inactivated bacteria. the authors have focused on the development of polybacterial vaccine with both specific and nonspecific immunostimulatory activity. the vaccine contains killed bacteria which most frequently occur as an etiological agent in urinary tract infections. determining for the selection of particular strains for the vaccine preparati ...199910493005
the facilitating effect of cigarette smoke on the colonization of instilled bacteria into the tracheal lumen in rats and the improving influence of supplementary vitamin e on this process.the aim of the present study was to investigate the direct influence of cigarette smoke on the bacterial colonization of the lower respiratory tract and the effect of supplementary vitamin e on the colonization of instilled bacteria into the trachea of rats that do not have the chronic airway pathology associated with smoking.199910489666
canine distemper virus infection in binturongs (arctictis binturong).two binturongs (arctictis binturong) kept in outdoor exhibits at everland zoological gardens in korea died within 10 days of the onset of clinical signs that included depression, dyspnoea, diarrhoea and convulsions. on necropsy, the significant gross findings were limited to the lungs and the gastrointestinal tract. proteus vulgaris was isolated from the lung of one animal. histopathologically, diffuse severe bronchointerstitial pneumonia with secondary bacterial infection was noted in the lungs ...199910486167
proteus mirabilis osteomyelitis of the ribs in a girl with sickle cell anaemia. 199910485315
paediatric nosocomial urinary tract infection at a regional hospital.from a total of 26,603 admissions to the paediatric wards, 1360 paediatric nosocomial urinary tract infections (pnuti) were identified during a 5-year retrospective chart review at the sfgh. the ages ranged from 3 days to 13 years, with 46% boys and 54% girls. the highest rates of pnuti per service per 100 admissions were seen in the nursery (11.28) followed by paediatric surgery (2.89) and paediatric medicine (2.86). although the greatest number of pnuti occurred in the nursery, comparison betw ...199910481961
investigation of influence of proteus mirabilis lps polysaccharide part on the murine immune cells activation.the biological activities were investigated of proteus mirabilis lipopolysaccharides (lpss) and their fragments, namely, the induction of nitric oxide (no) and interleukin 1 (il-1) synthesis by murine macrophages and the proliferation of murine spleen cells. the o-specific polysaccharide (f1), core oligosaccharide (f2) and lipid a were as effective as intact lps in stimulating murine macrophages to produce no. il-1 synthesis was also induced by all studied types of endotoxins (s, ra, re) and par ...199910470447
in vitro activity of newer broad spectrum beta-lactam antibiotics against enterobacteriaceae and non-fermenters: a report from austrian intensive care units. austrian carbapenem susceptibility surveillance group.we compared the in vitro activity of broad spectrum beta-lactam antibiotics against 573 gram-negative isolates (enterobacteriaceae and non-fermenters) collected between november 1996 and may 1997 from 9 laboratories serving intensive care units throughout austria. mic's (minimal inhibitory concentration) were obtained with the e-test for meropenem, imipenem, ceftazidime, cefepime, cefpirome and piperacillin/tazobactam. pseudomonas aeruginosa was the most frequently isolated organism (22%), follo ...199910467641
microbes and sequestered substances as mechanisms for disease: bartonella and l-forms as common global etiological agents.the pathogenicity of microbes may be determined by substances sequestered from blood and bound to their constituent lipid. the brain may not perceive substances sequestered by microbes, to interfere with control to maintain normal levels. pathological conditions can be induced as organisms exposed to antimicrobial substances/conditions and/or deprived of nutrients essential to cell wall synthesis, disintegrate to free lipid-bound compounds and produce l-forms that can deplete nutrients as they r ...199910465665
comparison of the beta-glucuronidase assay and the conventional method for identification of escherichia coli on eosin-methylene blue agar.the purpose of this study was to compare the imvic (indole, methyl red, voges-proskauer and citrate utilization) tests with the beta-glucuronidase (gud) assay for the identification of suspect escherichia coli on levine's eosin-methylene blue (emb) agar. after testing 258 suspect e. coli colonies from raw meat and meat products, 163 and 44 were found to be e. coli and non-e. coli, respectively, by both methods. nine isolates were imvic positive (i.e., + + - - or - + - -) but gud negative; among ...199710465032
antibacterial activity of ocimum gratissimum l. essential oil.the essential oil (eo) of ocimum gratissimum inhibited staphylococcus aureus at a concentration of 0.75 mg/ml. the minimal inhibitory concentrations (mics) for shigella flexineri, salmonella enteritidis, escherichia coli, klebsiella sp., and proteus mirabilis were at concentrations ranging from 3 to 12 microg/ml. the endpoint was not reached for pseudomonas aeruginosa (>=24 mg/ml). the mics of the reference drugs used in this study were similar to those presented in other reports. the minimum ba ...199910464416
antimicrobial resistance patterns in urinary isolates from nursing home residents. fifteen years of data reviewed.the antibiotic resistance patterns of gram-negative bacteria isolated from nursing home patients between 1983 and 1997 were analysed. escherichia coli was the most prevalent isolate (48%) followed by proteus spp. (26%) and other enterobacteriaceae (20%). during the study period, the susceptibility of e. coli decreased for co-trimoxazole (79% to 62%), increased for nitrofurantoin (79% to 91%) and remained unchanged for amoxycillin (41%). susceptibility to norfloxacin, available from 1990, decreas ...199910459818
proteus syndrome: a possible case associated to precocious puberty.we report a boy with possible proteus syndrome and precocious puberty. this appears to be the first report of this association.199910457862
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