Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| a mechanistic model for the uptake of waterborne strontium in the common carp (cyprinus carpio l.). | the release of radioactive strontium to the environment is of concern due to the strong accumulation of this calcium resembling element in the bone and other tissues. to predict the effects of changes in environmental conditions on the uptake of sr2+ and ca2+ by freshwater fish, a michaelis-menten type model is introduced that accounts for the effects of chemical speciation, hydrogen ion activity, and metal ion competition. the uptake kinetics were characterized in vivo from short-term exposure ... | 2001 | 11349276 |
| appetite-suppressing effects of urotensin i and corticotropin-releasing hormone in goldfish (carassius auratus). | fish urotensin i (ui), a member of the corticotropin-releasing hormone (crh) family of peptides, is a potent inhibitor of food intake in mammals, yet the role of ui in the control of food intake in fish is not known. therefore, to determine the acute effects of ui on appetite relative to those of crh, goldfish were given intracerebroventricular (i.c.v.) injections of carp/goldfish ui and rat/human crh (0.2-200 ng/g) and food intake was assessed for a 2-hour period after the injection. ui and crh ... | 2001 | 11340339 |
| simulation of acid-base condition and copper speciation in the fish gill microenvironment. | ph, alkalinity, and mucus content in the fish gill microenvironment of carp (cyprinus carpio) were measured by exposing fish to copper at various water ph levels using an apparatus which separates inspired and expired water. the relationship between ph levels inside and outside of the gill microenvironment, between ph and alkalinity, and between mucus secretion, ph, and copper exposure concentration were modeled. copper speciation in the surrounding water and in the fish gill microenvironment wa ... | 2001 | 11339403 |
| induction of specific cytotoxic t-cell activity against xenogeneic target cells in carp (cyprinus carpio). | to investigate the induction of cytotoxic t cells in carp (cyprinus carpio) after inoculation of fish with 2 xenogeneic line cells and to examine specificity of the cytotoxic activity. | 2001 | 11327471 |
| zn2+ differentially modulates signals from red- and short wavelength-sensitive cones to horizontal cells in carp retina. | the effects of zn2+ were studied while recording intracellularly from l-type horizontal cells (lhcs) in the isolated, superfused carp retina. in darkness, 25 microm zn2+ hyperpolarized lhcs and potentiated responses of these cells to 500 nm flashes, but decreased those to 680 nm flashes. zn2+ did not change photopic electroretinographic p iii responses. the differential modulation by zn2+ persisted when the zn2+-induced membrane hyperpolarization was compensated by lowering ca2+ concentration in ... | 2001 | 11325351 |
| histopathology of viremia-associated ana-aki-byo in combination with aeromonas hydrophila in color carp cyprinus carpio in japan. | a disease in which 'viremia-associated ana-aki-byo' is combined with an aeromonas hydrophila infection currently occurs and is highly transmissible in color carp cyprinus carpio in japan. in the present study, to determine the interrelation between a corona-like virus and a. hydrophila, we conducted transmission trials by cohabiting naturally diseased carp with healthy carp with skin that had been slightly damaged artificially. experimentally exposed fish successfully replicated the combination ... | 2001 | 11324811 |
| [zinc modulation of gaba receptors expressed in xenopus oocytes injected with carp brain poly(a)+ rna]. | two types of gaba receptors, gabaa receptors (approximately 85%) and gabac-like receptors (approximately 15%), were expressed in xenopus oocytes after injection of poly(a)+ rna with carp (carassius carassius) brain. using voltage-clamp technique and pharmacological methods, the effects of zinc on the two receptors were studied. it was found that zinc modulation of both gabaa and gabac-like receptors by zinc was inhibitory and reversible with the former being stronger than the latter as indicated ... | 1998 | 11324566 |
| seasonal population dynamics of neoechinorhynchus qinghaiensis in the carp, gymnocypris przewalskii przewalskii, from qinghai lake, china. | studies on the seasonal population dynamics of neoechinorhynchus qinghaiensis (acanthocephala: neoechinorhynchidae) in its fish host gymnocypris przewalskii przewalskii in the qinghai lake, china, were carried out with samples taken in may 1991, august 1992, november 1992 and february 1993. prevalences were higher than 44% in all seasons. the mean intensity of infection was above 124 worms per fish. the maximum intensity of worms recovered from a single fish was 1402 in the autumn of 1992. diffe ... | 2001 | 11316478 |
| forskolin modulation of desensitization at gaba(a) and glycine receptors is not mediated by camp-dependent protein kinase in isolated carp amacrine-like cells. | the effects of forskolin on gamma-aminobutyric acid type-a (gaba(a)) and glycine receptors in amacrine-like cells of carp (carassius auratus) retina were studied using patch-clamp techniques. application of 50 microm forskolin markedly accelerated the desensitization of whole-cell responses induced by 100 microm gaba or glycine without changing the peak amplitude of the response. both 8-bromoadenosine 3',5'-cyclic monophosphate (8-br-camp) and 3-isobutyl-1 -methylxanthine (ibmx) (500 microm) fai ... | 2001 | 11316256 |
| starvation-induced pathobiology in the gut of carp (cyprinus carpio l.). | in order to assess the effects of starvation on intestinal intraepithelial lymphocytes (i-iel) count and histological changes of the carp gut mucosa, one group of fish (n = 10) were fed commercially prepared standard diets and another group of fish (n = 10) were starved for 4 weeks. carp starved for 4 weeks developed enteropathy, comprising folds atrophy, stratum compactum hyperplasia, significant periodic-acid-schiff (pas)-positive (p < 0.00001), but not alcian blue (alb)-positive, goblet cell ... | 2003 | 11314585 |
| identification of beta-endorphins in the pituitary gland and blood plasma of the common carp (cyprinus carpio). | carp beta-endorphin is posttranslationally modified by n-terminal acetylation and c-terminal cleavage. these processes determine the biological activity of the beta-endorphins. forms of beta-endorphin were identified in the pars intermedia and the pars distalis of the pituitary gland of the common carp (cyprinus carpio), as well as the forms released in vitro and into the blood. after separation and quantitation by high performance liquid chromatography (hplc) coupled with radioimmunoassay, the ... | 2001 | 11312144 |
| analysis of spontaneous epscs in retinal horizontal cells of the carp. | spontaneous excitatory postsynaptic currents (sepscs) were recorded under whole-cell voltage clamp from carp type 1 horizontal cells (h1 cells) uncoupled by dopamine in retinal slices. red light steps, which hyperpolarise cones and reduce glutamate release, induced outward current responses accompanied by a suppression of sepscs. sepscs decayed exponentially with a mean time constant of 0.71+/-0.07 ms and had a reversal potential near 0 mv. power spectral analysis of sepscs revealed a similar de ... | 2001 | 11311408 |
| a new syndrome with craniofacial and skeletal dysmorphisms and developmental delay. | we report a 16-year-old boy with multiple craniofacial and skeletal dysmorphic features including brachycephaly, acrocephaly, hypertelorism, wide palpebral fissures, broad nose, anteverted nares, broad columella, long and smooth philtrum, thin upper lip, macrostomia, carp-like mouth, micrognathia, low-set and posteriorly angulated ears with small and abnormal pinnae, a low posterior hairline, a short neck, hypoplastic and widely-spaced nipples, multiple severe pterygia, an umbilical hernia, meta ... | 2001 | 11311002 |
| myopalladin, a novel 145-kilodalton sarcomeric protein with multiple roles in z-disc and i-band protein assemblies. | we describe here a novel sarcomeric 145-kd protein, myopalladin, which tethers together the cooh-terminal src homology 3 domains of nebulin and nebulette with the ef hand motifs of alpha-actinin in vertebrate z-lines. myopalladin's nebulin/nebulette and alpha-actinin-binding sites are contained in two distinct regions within its cooh-terminal 90-kd domain. both sites are highly homologous with those found in palladin, a protein described recently required for actin cytoskeletal assembly (parast, ... | 2001 | 11309420 |
| molecular cloning of preproinsulin cdnas from several osteoglossomorphs and a cyprinid. | several preproinsulin cdnas were isolated and characterized from four members of the osteoglossomorpha (an ancient teleost group); osteoglossum bicirrhosum (arawana), pantodon buchholzi (butterfly fish), notopterus chitala (feather fin knife fish), hiodon alosoides (goldeye) and gnathonemus petersii (elephantnose). in addition, we isolated and characterized the preproinsulin cdna from catostomus commersoni (white sucker, as a representative of a generalized teleost). the comparative analysis of ... | 2001 | 11306171 |
| differential screening and characterization analysis of the egg envelope glycoprotein zp3 cdnas between gynogenetic and gonochoristic crucian carp. | gynogenetic silver crucian carp, carassius auratus gibelio, is an intriguing model system. in the present work, a systemic study has been initiated by introducing suppression subtractive hybridization technique into this model system to identify the differentially expressed genes in oocytes between gynogenetic silver crucian carp and its closely related gonochoristic color crucian carp. five differential cdna fragments were identified from the preliminary screening, and two of them are zp3 homol ... | 2001 | 11305321 |
| a peptide derived from the c-terminal part of a plant cysteine protease folds into a stack of two beta-hairpins, a scaffold present in the emerging family of granulin-like growth factors. | a 35 amino acid residue peptide corresponding to the n-terminal subdomain of the granulin-like repeat from rice oryzain beta was synthesized and regioselectively oxidized to produce a species with a [1-3, 2-4] disulfide-pairing pattern. the resulting peptide was studied in solution using nmr and was shown to adopt the tertiary topology of a stack of two beta-hairpins found in the emerging family of granulin-like growth factors. because of the longer second beta-hairpin, the overall conformation ... | 2001 | 11298924 |
| signals from cone photoreceptors to l-type horizontal cells are differentially modulated by low calcium in carp retina. | ca2+ plays crucial roles in both phototransduction and calcium-dependent glutamate release from the photoreceptor terminal. modulation, by lowering extracellular ca2+, of red-sensitive (r-) and short wavelength-sensitive (s-) cone-driven light responses of l-type horizontal cells (lhcs) was studied in the isolated superfused carp retina using intracellular recording techniques. low ca2+ (nominally ca2+-free) ringer's reduced responses of lhcs to both green (500 nm) and red (680 nm) flashes in da ... | 2001 | 11298802 |
| fine structure of the skin cells of a stenohaline freshwater fish cyprinus carpio exposed to diluted seawater. | seawater diluted to half (1.750% salinity) is lethal for adult carps after 3 h and 15 min. at lower salinities (0.350%-0.875%), the fish survived for longer periods, but only 0.175% salinity was innocuous. in carps, adapted to 0.175% salinity, the secretory activity of pavement cells was very high and their external ridges flattened or even disappeared. mucus secretion was conspicuous, characterized by holocriny of old cells and apparition of young ones in large numbers. the intracellular mucus ... | 2001 | 11292170 |
| ammonia-induced cellular and immunological changes in juvenile cyprinus carpio infected with the blood fluke sanguinicola inermis. | immunological and structural changes in the thymus and pronephros of cyprinus carpio infected with the blood fluke, sanguinicola inermis for 30 days, and exposed to 0.5 mg nh4+/1 for 48 and 168 h were investigated. ultrastructural observations revealed cell disruption and highly vacuolated cytoplasm in the thymus. of the cells that remained intact there was a significant increase in thrombocytes after 48 h exposure to the pollutant. in addition, there was a decrease in lymphocytes following expo ... | 2001 | 11289070 |
| micronuclei monitoring of fishes from lake paranoá, under influence of sewage treatment plant discharges. | fish micronuclei tests (mn) were used to evaluate the ability of wastewater from two municipal sewage treatment plants that empty into lake paranoá to cause genetic damage. there were no significant differences in the frequencies of micronuclei between the control and hypertrophic areas. in contrast, cyclophosphamide and mitomycin c, used to test the sensitivity of the biological assay, significantly increased the micronuclei counts in tilapia rendalli, oreochromis niloticus and cyprinus carpio, ... | 2001 | 11287296 |
| unique biochemical nature of carp retinol-binding protein. n-linked glycosylation and uncleavable nh2-terminal signal peptide. | retinol transport and metabolism have been well characterized in mammals; however, very little is known in fish. to study the mechanism by which fish retinol-binding protein (rbp) is able to remain in plasma besides its small molecular size, we isolated rbp cdna from a carp liver cdna library. comparison of the deduced amino acid sequence with that of known vertebrate rbps showed that carp rbp has high homology to the other cloned vertebrate rbps, but it lacks the cooh-terminal tetrapeptide, rnl ... | 2001 | 11278316 |
| mercury in sediment and fish from north mississippi lakes. | sediments and/or fish were collected from sardis, enid and grenada lakes, which are located in three different watersheds in north mississippi, in order to assess mercury contamination. the mean total mercury concentration in sediments from enid lake in 1997 was 0.154 mg hg/kg, while 1998 sediment concentrations in sardis, enid and grenada lakes were 0.112, 0.088 and 0.133 mg hg/kg, respectively. sediment mercury concentrations in 1999 were similar in all three lakes but, generally lower than 19 ... | 2001 | 11272915 |
| bioenergetics and rna/dna ratios in the common carp (cyprinus carpio) under hypoxia. | hypoxia caused by eutrophication occurs over large areas in aquatic systems worldwide. common carp (cyprinus carpio) exposed to hypoxia (1 mg.o2.l(-1) and 2 mg.o2.l(-1)) for 1 week showed a significant reduction in feeding rate, respiration rate, faecal production and nitrogenous excretion compared to those maintained at normoxia (7 mg.o2.l(-1)). fish exposed to hypoxia showed negative scope for growth (sfg), but no significant difference in the specific growth rate was revealed after 1 week in ... | 2001 | 11263726 |
| long-term cortisol treatment inhibits pubertal development in male common carp, cyprinus carpio l. | the onset and regulation of puberty is determined by functional development of the brain-pituitary-gonad (bpg) axis. stress has been shown to interfere with reproduction and the functioning of the bpg axis. the response to chronic and severe stress may require much energy and force the organism to make adaptive choices. energy that is normally available for processes like growth, immune response, or reproduction will be channeled into restoration of the disturbed homeostasis. cortisol plays a ke ... | 2001 | 11259251 |
| cytochrome p450 (p450) isoforms expression, p450 concentration, monooxygenase activities, reactive oxygen species formation, lipid peroxidation, and glutathione content in wild catch carp and tench liver--influence of a two weeks exposure to phenobarbital. | carps, both sexes, 3 years old, weighing about 1 kg, and tenches of both sexes, 6 years old, weight about 250 g, were caught from a thuringian lake without industrial pollution in november 1995 (fish without food uptake, water temperature at about 10 degrees c) and kept for 2 weeks in basins with clean water and addition of 0, 0.1, 1.0 or 10.0 mg/l phenobarbital-na (pb). the concentration of pb was controlled during and at the end of the exposure period. the animals were fed pellets, but no food ... | 2001 | 11256753 |
| biochemical and morphological changes in carp (cyprinus carpio l.) liver following exposure to copper sulfate and tannic acid. | as a consequence of human activity various toxicants reach the aquatic ecosystems; humics may interact with them and may change their toxicity. many fish are exposed to a considerable concentration of humics and pollutants. because of paucity of data on the biochemical action of tannins in the presence of the fungicide cuso4 a comparative study was undertaken. the alterations of redox-parameters in carp liver were monitored and tissue necrosis was followed by measuring the plasma transaminase ac ... | 2001 | 11255117 |
| tissue-specific expression of two metallothionein genes in common carp during cadmium exposure and temperature shock. | two metallothionein cdna isoforms (mt-1 and mt-2) were isolated from carp (cyprinus carpio) by rt-pcr. sequence analysis of the cdnas revealed two amino acid differences between the coding regions and markedly different 3'-untranslated ends. gene-specific primers were selected and used in rt-pcr reactions to measure the basal mt-1 and mt-2 mrna levels and to follow the inducer-specific expression of mt genes in different tissues during in vivo studies. in the brain and muscle, the uninduced leve ... | 2001 | 11255116 |
| an immunocytochemical study of the pituitary gland of the white seabream (diplodus sargus). | the adenohypophysis of the white seabream (diplodus sargus) was studied using histochemical and immunocytochemical techniques. the adenohypophysis was composed of rostral pars distalis, proximal pars distalis and pars intermedia. prolactin (anti-chum salmon prolactin positive) and adrenocorticotropic (anti-human acth positive) cells were found in the rostral pars distalis. prolactin cells were organized into follicles, while acth cells were arranged in cords around neurohypophyseal tissue branch ... | 2000 | 11254089 |
| effects of exhausting exercise and catecholamines on k+ balance, acid-base status and blood respiratory properties in carp. | the potential role of adrenergic mechanisms in the recovery of potassium balance and acid-base status following 5 min of exhausting exercise was studied in carp. the extracellular metabolic h+ load after exercise matched the lactate load, suggesting similar release rates of h+ and lactate from white muscle. blockage of alpha-adrenoceptors by phentolamine or beta-adrenoceptors by propranolol neither influenced absolute magnitudes nor recovery kinetics of extracellular h+ and lactate loads. the ar ... | 1998 | 11253799 |
| early expression of pituitary adenylate cyclase-activating polypeptide and activation of its receptor in chick neuroblasts. | to investigate the involvement of pituitary adenylate cyclase- activating polypeptide (pacap) and gh-releasing factor (grf) during early chick brain development, we established neuroblast- enriched primary cell cultures derived from embryonic day 3.5 chick brain. we measured increases in camp generated by several species-specific forms of the peptides. dose-dependent increases up to 5-fold of control values were measured in response to physiological concentrations of human/salmon, chicken, and t ... | 2001 | 11250943 |
| monosaccharide uptake in common carp (cyprinus carpio) epc cells is mediated by a facilitative glucose carrier. | in most animal cells, transport of monosaccharides across the plasma membrane is mediated by glucose transporters (glut). mammals express at least five distinct transporters (gluts 1--5), which are well characterised both functionally and genetically. in contrast, the glucose transport system of fish remains poorly studied. here we report studies of hexose uptake in carp epc cells and cloning of a glucose transporter cdna from these cells. transport of radio-labelled methylglucose (3-omg) follow ... | 2001 | 11250543 |
| effects of zinc on lipids of erythrocytes from carp (cyprinus carpio l.) acclimated to different temperatures. | we compared the effect of zinc (0.01, 0.1, 0.5 and 1 mm) at two temperatures (5 and 20 degrees c) on erythrocytes from summer and winter acclimatised carp. an increase in temperature from 5 to 20 degrees c increased the unsaturation index (ui) and relative proportion (ui/sfa) of unsaturated to saturated fatty acids in total lipids of the red cells. at 5 degrees c, the unsaturation index of phosphatidylcholine (pc), phosphatidylserine (ps), phosphatidylethanolamine (pe) and phosphatidylinositol ( ... | 2000 | 11246505 |
| comparative study of acetylcholinesterase and butyrylcholinesterase in brain and serum of several freshwater fish: specific activities and in vitro inhibition by ddvp, an organophosphorus pesticide. | a comparative study of specific activities and in vitro inhibition of brain and serum acetylcholinesterase (ache; ec 3.1.1.7) and serum butyrylcholinesterase (bche; ec 3.1.1.8) by ddvp, an organophosphorus pesticide, was conducted in 11 freshwater teleost species belonging to four families (cyprinidae; common carp cyprinus carpio, bream abramis brama, blue bream a. ballerus, white bream blicca bjoerkna, roach rutilus rutilus, bleak alburnus alburnus, ide leuciscus idus; percidae: perch perca flu ... | 2000 | 11246494 |
| melatonin modulates gamma-aminobutyric acid(a) receptor-mediated currents on isolated carp retinal neurons. | modulation by melatonin of gamma-aminobutyric acid(a) (gaba(a)) receptor-mediated responses was studied in bipolar and amacrine-like cells acutely isolated from carp retina, using the whole-cell patch-clamp technique. melatonin of 1 mm accelerated desensitization of the gaba(a) receptors at both bipolar and amacrine-like cells. in addition, 1 mm melatonin hardly changed the gaba(a) receptor-mediated response amplitude of bipolar cells, while it increased or decreased that of amacrine-like cells, ... | 2001 | 11239714 |
| an electrophysiological test of the effect of the temporal pattern of light adaptation on teleost h1 type horizontal cell plasticity. | the possible importance of the temporal pattern of photon delivery in light adaptation-induced physiological plasticity in the outer retina of carp was tested by intracellular recording. steady and flicker (3 hz) background adaptation was applied whilst recording chromatic voltage responses of h1 type horizontal cells (hcs) to 680 and 440 nm full-field test flashes (generating response amplitudes of v(r) and v(b), respectively). a third parameter v(b)/v(r) (b/r) was calculated as an indicator of ... | 2001 | 11239704 |
| dynamics of (cd,zn)-metallothioneins in gills, liver and kidney of common carp cyprinus carpio during cadmium exposure. | cadmium concentrations, (cd,zn)-metallothionein (mt) concentrations, mt synthesis and the relative amounts of cadmium bound to (cd,zn)-mts were determined in gills, liver and kidney of common carp cyprinus carpio exposed to 0, 0.5 microm (0.06 mg.l(-1)), 2.5 microm (0.28 mg.l(-1)) and 7 microm (0.79 mg.l(-1)) cd for up to 29 days. cadmium accumulation was in the order kidney > liver > gills. control levels of hepatic (cd,zn)-mt were four times higher compared to those of gills and kidney. no inc ... | 2001 | 11239687 |
| [effect of stannius corpuscles homogenates of carp on plasma calcium and phosphate in mice]. | 2000 | 11236681 | |
| hematology and clinical chemistry of cyprinid fish. common carp and goldfish. | evaluation of the clinical status in aquatic species is compromised by the limited diagnostic techniques that can be performed in these species. the hematologic and plasma chemistry parameters can provide predictive information, although these parameters can be highly variable owing to the influence of various intrinsic and extrinsic factors. however, these parameters are fairly stable in acclimated, well-managed fish if stress is reduced during collection and samples are properly collected and ... | 1999 | 11229053 |
| quantification of rainbow trout (oncorhynchus mykiss) zona radiata and vitellogenin mrna levels using real-time pcr after in vivo treatment with estradiol-17 beta or alpha-zearalenol. | estrogen receptor-mediated induction of zona radiata (zr) and vitellogenin (vtg) mrna and protein in rainbow trout (oncorhynchus mykiss) was compared to assess their utility as biomarkers for exposure to estrogenic compounds. partial sequences of rainbow trout zr and beta-actin were cloned by reverse transcriptase polymerase chain reaction (rt-pcr) using degenerate primers based on conserved regions across a number of species. a 549 bp fragment of the rainbow trout zr-gene showed a high degree o ... | 2000 | 11226827 |
| nerve growth factor induces light adaptive cellular and synaptic plasticity in the outer retina of fish. | recent evidence suggests that neurotrophins can be involved in short-term synaptic plasticity in parts of the central nervous system. in the present study, the possible role of nerve growth factor (ngf) in inducing morphologic (cellular and subcellular) changes in the outer retina of carp was assessed. the effects of ngf on cone photomechanical movements (pmms) and horizontal cell (hc) spinule formation were measured. ngf-induced cone contraction and formation of hc spinules in the dark-adapted ... | 2001 | 11223810 |
| stress responses and changes in protein metabolism in carp cyprinus carpio during cadmium exposure. | stress responses and changes in protein metabolism were studied in common carp cyprinus carpio exposed to 0, 0.8, 4, and 20 microm cadmium (cd) over a 29-day period. blood and other tissue samples were taken after 4 and 29 days of exposure. the highest cd concentration proved to be lethal to the fish, resulting in 100% mortality after 21 days of exposure. cd accumulated in the tissues in the following order: kidney>liver>gills. blood hematocrit, blood hemoglobin, plasma glucose, plasma lactate, ... | 2001 | 11222034 |
| molecular cloning of the complement (c1r/c1s/masp2-like serine proteases from the common carp (cyprinus carpio). | the classical pathway of complement composed of c1, c4, and c2 is an antibody-dependent activation cascade that is present in jawed vertebrates. c1 is a ca2+-dependent complex of c1q, c1r, and c1s, and analogous to an initiation complex of the lectin pathway of complement, which consists of the mannose-binding lectin (mbl) homologous to c1q and the mbl-associated serine proteases (masps) homologous to c1r and c1s. thus divergence of clq and mbl and that of c1r, c1s and the masps are considered t ... | 2001 | 11220628 |
| metals, boron, and selenium in neosho madtom habitats in the neosho river in kansas, u.s.a. | in 1991 and 1992 we determined the levels of metals, arsenic, boron, and selenium in the neosho river drainage in southeastern kansas, the primary habitat for the threatened neosho madtom (noturus placidus). we evaluated concentrations in sediments, mussels (quadrula pustulosa and q. metanevra), and fish (percina phoxocephala, cyprinus carpio, and ictiobus bubalus) from three sites on the neosho river and one site on the cottonwood river. we also evaluated contaminant concentrations in c. carpio ... | 2001 | 11214445 |
| copy number related transgene expression and mosaic somatic expression in hemizygous and homozygous transgenic tilapia (oreochromis niloticus). | three lines of transgenic tilapia (oreochromis niloticus) fish were generated with a construct containing a lacz reporter gene spliced to a 4.7 kb 5' regulatory region of a carp beta actin gene. all these three lines contain different copy numbers of transgenes and the levels of lacz expression were found to be related to transgene copy number. mosaic patterns of somatic lacz expression were observed in these three lines which differed between lines but were consistent within a line. we also obs ... | 2000 | 11206970 |
| [rodlet cells of fish in calcium deficiency in the external environment]. | the ultrastructure of rodlet cells from the heat kidney of the carp cyprinus carpio l. and tilapia oreochromis mossambicus peters has been studied. all the investigated cells displayed a common structure. the number of rodlet cells increased after exposure of fishes in ca(2+)-free water or in the water supplemented with nh4+ or cd2+. the latter were found to suppress ca(2+)-absorption from the environment. | 2000 | 11204648 |
| comparative metabolism of phenanthridine by carp (cyprinus carpio) and midge larvae (chironomus riparius). | abiotic transformation of azaarenes in the environment has been analysed extensively, but metabolism is less well described. to further elucidate preliminary observations of interspecific differences in azaarene metabolism by aquatic organisms, phenanthridine biotransformation by midge larvae and carp was studied. in both experiments, 6(5h)-phenanthridinone (phenanthridone) was found as an important metabolite. the fish were clearly capable of metabolising phenanthridine, but in the midge experi ... | 2001 | 11202649 |
| the feedforward component in depolarizing red responses of r/g horizontal cells in carp retina. | light responses of r/g chromaticity-type horizontal cells (r/g hcs) and luminosity-type horizontal cells (lhcs) were intracellularly recorded in isolated superfused carp retina, and the response dynamics analyzed. the results revealed that (1) no significant difference in delay was detected between r/g hc red and green responses; (2) the rising speed was quicker for r/g hc depolarizing red responses compared to that of its hyperpolarizing green responses; and (3) dynamic characteristics of r/g h ... | 2014 | 11193108 |
| concentrations and frequencies of polychlorinated biphenyl congeners in a native american population that consumes great lakes fish. | polychlorinated biphenyl congener profiles were examined in serum samples from 61 native american (ojibwa) volunteers who regularly consumed fish harvested from the great lakes region. a total of 93 peaks are reported which represent 126 individual chlorobiphenyls. | 2000 | 11192460 |
| [molecular evolution of two c-myc genes of a tetraploid teleost, the common carp]. | 2000 | 11187801 | |
| reactive oxygen species uncouple external horizontal cells in the carp retina and glutathione couples them again. | we have investigated the effect of free radicals on the electrical gap junctions between horizontal cells in the carp retina. in our previous study, l-buthionine sulfoximine, an inhibitor of glutathione synthesis, caused uncoupling of horizontal cells four days after injection. in the present study, we have used paraquat, a generator of exogenous reactive oxygen species, to investigate whether it was the depletion of glutathione or an increase in the level of reactive oxygen species which result ... | 2001 | 11182257 |
| differential in vitro suppressive effects of steroids on leukocyte phagocytosis in two teleosts, tilapia and common carp. | the objectives of this study were to investigate the potential roles of cortisol and gonadal steroids in the phagocytic activity of peripheral blood leukocytes in two teleosts, tilapia (oreochromis niloticus x o. aureus) and common carp (cyprinus carpio). an in vitro microtiter plate assay, measuring incorporation of fitc-latex beads into peripheral blood leukocytes, was developed for the first time in teleosts. peripheral blood leukocytes were cultured in al medium with tested compounds in a mi ... | 2001 | 11178882 |
| genotoxicity of textile dye effluent on fish (cyprinus carpio) measured using the comet assay. | 2001 | 11178658 | |
| giant mitochondria as possible bioindicators of environmental injuries in fish liver. | the effect of hypoxia (80 phg) and simultaneously applied paraquat (1,1'-dimethyl-4,4-bipyridynum dichloride) was investigated on carp liver using electron microscopic methods. the appearance of giant mitochondria was the most conspicuous alteration in the liver cells. most of the giant mitochondria were elongated and rod-shaped, often arranged side by side forming clusters beside the nucleus. crook-like and irregular forms also occured among giant mitochondria. the lenght of the giant mitochond ... | 1997 | 11173631 |
| cadmium-binding to transferrin in the plasma of the common carp cyprinus carpio. | in this study, it was investigated by autoradiography with radioactive cadmium after western blotting of two-dimensional electrophoresis gels, to which proteins cadmium is mainly bound in plasma of common carp cyprinus carpio. the obtained results demonstrate that in carp plasma, cadmium is primarily bound to two high molecular weight proteins. relative small amounts are bound to a protein with m(r) approximately 60000. the other metal-binding protein, with m(r) approximately 70000 and pi approx ... | 2001 | 11166673 |
| regulation of interleukin 1 beta rna expression in the common carp, cyprinus carpio l. | the intron-exon organisation of the carp il-1beta gene consists of 2455bp and comprises seven exons. three il-1beta rna transcripts have been found in carp: (1) a fully spliced product; (2) exon 1-7 with introns 5 and 6; and (3) exon 1-7 with intron 5 only. the intron-containing products probably represent partially spliced transcripts. il-1beta mrna expression in carp was semi-quantitatively analysed by rt-pcr in multiple organs, including brain and pituitary. constitutive expression of the il- ... | 2001 | 11164885 |
| bioavailability of cd to the common carp, cyprinus carpio, in the presence of humic acid. | the uptake of cadmium by the common carp, cyprinus carpio, was studied in chemically defined freshwater in the absence and presence of commercial humic acid. this was done to evaluate whether the cadmium uptake by carp in the presence of humic acid was related to the ambient cd2+ -ion activity or whether the complexed metal also contributed to the uptake. uptake of cd during a 3-h period of exposure was used as a measure of the biological availability of the metal. the uptake rate data for cd in ... | 2001 | 11163427 |
| purification of a novel myofibril-bound serine proteinase inhibitor (mbspi) from the skeletal muscle of lizard fish. | a novel myofibril-bound serine proteinase inhibitor (mbspi) was purified to homogeneity from the skeletal muscle of lizard fish (saurida wanieso). purification was carried out by ammonium sulfate fractionation, followed by column chromatographies on deae-sephacel, sp-sepharose and sephadex g-150. mbspi was purified 7.7-fold starting from the deae-sephacel fraction, with a yield of 0.2%. it is a monomeric protein with the molecular mass of 50 kda as estimated by sds-page and gel filtration. mbspi ... | 2001 | 11163300 |
| observations on the effects of caged carp culture on water and sediment metal concentrations in lake kasumigaura, japan. | the concentrations of 24 elements in the sediment and associated water column were monitored at two sites, one an area of intensive cage culture of carp, the other a wild site far from known cage culture areas, in lake kasumigaura, japan, between september 1994 and september 1995. the concentrations of most elements in lake kasumigaura are mostly sub-parts per billion, except those for ca, fe, k, mg, na, p, and si. the concentrations of cd, co, cu, mn, ni, pb, v, and fe in lake kasumigaura are h ... | 2001 | 11161684 |
| effects of essential fatty acid deficiency and supplementation with docosahexaenoic acid (dha; 22:6n-3) on cellular fatty acid compositions and fatty acyl desaturation in a cell culture model. | the desaturation of [1-(14)c] 18:3n-3 to docosahexaenoic acid (dha; 22:6n-3) is enhanced in an essential fatty acid deficient cell line (epc-efad) in comparison with the parent cell line (epc) from carp. in the present study, the effects of dha on lipid and fatty acid compositions, and the metabolism of [1-(14)c]18:3n-3 were investigated in epc-efad cells in comparison with epc cells. dha supplementation had only relatively minor effects on lipid content and lipid class compositions in both epc ... | 2001 | 11161581 |
| cryopreservation of sperm in common carp cyprinus carpio: sperm motility and hatching success of embryos. | in this study, fish sperm cryopreservation methods were elaborated upon for ex situ conservation of nine strains of bohemian common carp. common carp sperm were diluted in kurokura medium and chilled to 4 degrees c and dimethyl sulfoxide was added. cryotubes of sperm with media were then cooled from +4 to -9 degrees c at a rate of 4 degrees c min(-1) and then from -9 to -80 degrees c at a rate of 11 degrees c min(-1), held for 6 min at -80 degrees c, and finally transferred into liquid n(2). the ... | 2000 | 11161556 |
| edwardsiella tarda mutants defective in siderophore production, motility, serum resistance and catalase activity. | edwardsiella tarda is a gram-negative bacterium that causes a systemic infection, edwardsiellosis, in fish. the virulence factors of this pathogen and its genetic determinants have not been systematically examined. in this study, tnphoa transposon mutagenesis was used to construct a library of 440 alkaline phosphatase (phoa(+)) fusion mutants from a total of 400000 transconjugants derived from ed. tarda ppd130/91. this library included genes for secreted and membrane-associated proteins normally ... | 2001 | 11158362 |
| does anoxia induce cell swelling in carp brains? in vivo mri measurements in crucian carp and common carp. | although both common and crucian carp survived 2 h of anoxia at 18 degrees c, the response of their brains to anoxia was quite different and indicative of the fact that the crucian carp is anoxia tolerant while the common carp is not. using in vivo t(2) and diffusion-weighted magnetic resonance imaging (mri), we studied anoxia induced changes in brain volume, free water content (t(2)), and water homeostasis (water diffusion coefficient). the anoxic crucian carp showed no signs of brain swelling ... | 2001 | 11152713 |
| uptake and processing of biofilm and free-cell vaccines of aeromonas hydrophila in indian major carps and common carp following oral vaccination--antigen localization by a monoclonal antibody. | uptake and processing of biofilm (bf) and free-cell (fc) vaccines of aeromonas hydrophila were studied in the indian major carps catla catla catla, and rohu labeo rohita and in the common carp cyprinus carpio following a single dose oral vaccination of 10(11) cfu g(-1) fish. fish were sampled at 0.5, 1, 3, 6, 12, 24 h and at 2, 3, 5 and 10 d following vaccination and antigen localization was studied in the gut, kidney and spleen employing monoclonal antibody based immunofluorescence and immunope ... | 2000 | 11145450 |
| transforming growth factor-beta/smads signaling induces transcription of the cell type-restricted ankyrin repeat protein carp gene through caga motif in vascular smooth muscle cells. | transforming growth factor (tgf)-beta plays a major role in the development of vascular diseases. despite the pleiotropic effects of tgf-ss on vascular smooth muscle cells (vsmcs), only a few genes have been characterized as direct targets of tgf-beta in vsmcs. cardiac ankyrin repeat protein (carp) has been thought to be expressed exclusively in the heart. in the present study, we showed that carp is expressed in the vasculature after balloon injury and in cultured vsmcs in response to tgf-beta. ... | 2001 | 11139470 |
| transforming growth factor-beta (tgf-beta) and vascular disease: carp as a putative tgf-beta target gene in the vessel wall. | 2001 | 11139465 | |
| muscle regulatory factor gene: zebrafish (danio rerio) myogenin cdna. | myogenin is one of the basic helix-loop-helix proteins that regulate muscle-specific gene expression. using reverse transciption-polymerase chain reaction (rt-pcr), 5'- and 3'-rapid amplification of cdna ends (race), zebrafish myogenin cdna was cloned from mrna of embryos at 10-96 h post-fertilization. the cdna, at 1384 base pairs (bp), contained a 771-bp open reading frame with 113- and 500-bp flanking regions at the 5'- and 3'-ends, respectively. the deduced amino acid sequences of zebrafish m ... | 2000 | 11126756 |
| detection of plasmalogen from plasma low density lipoprotein and high density lipoprotein in carp, cyprinus carpio, and rainbow trout, oncorhynchus mykiss. | the study revealed the presence of plasmalogens in the low density lipoprotein (ldl) and high density lipoprotein (hdl) of the fish. the composition of the plasmalogen in the carp plasma ldl phospholipids was 0.94 and 0.23% in the hdl; the ldl phospholipids in the rainbow trout were 0.44% and the hdl was 0.18%. aldehydes from the plasmalogen were derivatized with dansylhydrazides and separated by high performance liquid chromatography (hplc). their presence was detected using a fluorescence dete ... | 2000 | 11118943 |
| the distribution of neuropeptide y-immunoreactive neurons and nerve fibers in the forebrain of the carp cyprinus carpio l. | the present study reports the distribution of neuropeptide y (npy)-immunoreactive neurons and fibers in the forebrain of the adult carp cyprinus carpio l. serial nissl-stained sections were used for cytoarchitecture and identification of anatomical structures. immunostaining of npy-containing neurons and fibers was used as neurochemical marker and tool for comparison with other species, including the goldfish. the general outline of the cytoarchitecture of the carp forebrain is similar to that o ... | 2000 | 11118806 |
| chlorinated hydrocarbons and biomarkers of exposure in wading birds and fish of the lower rio grande valley, texas. | during 1997 we evaluated reproductive success in colonial water birds nesting in the lower rio grande valley (lrgv), texas, and correlated success with concentrations of contaminants in eggs. we also measured steroid hormones and gonadosomatic index (gsi) as biomarkers of endocrine effects in common carp (cyprinus carpio). nest and fledging success of green herons (butorides virescens) and great egrets (ardea alba) were similar to those found in other parts of north america; however, nesting suc ... | 2001 | 11116345 |
| products of proteolytic cleavage of transferrin induce nitric oxide response of goldfish macrophages. | enzymatic cleavage product of transferrin induced the production of nitric oxide (no) by lps-stimulated goldfish macrophages. a no-inducing factor was purified from the supernatants of mitogen-stimulated goldfish kidney leukocytes using fast performance liquid chromatography (fplc) and the purified proteins analyzed by microcapillary reverse-phase hplc nano-electrospray tandem mass spectrometry. the proteins were identified as truncated forms of transferrin, having approximate molecular weights ... | 2001 | 11113281 |
| acute and chronic influence of temperature on red blood cell anion exchange. | unidirectional (36)cl(-) efflux via the red blood cell anion exchanger was measured under cl(-) self-exchange conditions (i.e. no net flow of anions) in rainbow trout oncorhynchus mykiss and red-eared freshwater turtle trachemys scripta to examine the effects of acute temperature changes and acclimation temperature on this process. we also evaluated the possible adaptation of anion exchange to different temperature regimes by including our previously published data on other animals. an acute tem ... | 2001 | 11104709 |
| myxobolus intrachondrealis sp. n. (myxosporea: myxobolidae), a parasite of the gill cartilage of the common carp, cyprinus carpio. | a species not identifiable with any of the about 23 myxobolus species recorded from the common carp so far, was detected in the gills of one- and two-summer-old specimens of the common carp ((cyprinus carpio l.) cultured in pond farms in hungary. the strictly tissue-specific plasmodia of the parasite were located, surrounded by hyaline cartilage cells, in the chondrous substance of the terminal parts of the gill arches and in the cartilage structure ventrally connecting the gill arches. the spor ... | 2000 | 11104143 |
| physiological induction of a beta-adrenergic receptor kinase inhibitor transgene preserves ss-adrenergic responsiveness in pressure-overload cardiac hypertrophy. | transgenic mice with constitutive myocardium-targeted expression of a peptide inhibitor of the ss-adrenergic receptor kinase (ssarkct) have increased in vivo cardiac function and enhanced ss-adrenergic receptor (ssar) responsiveness. | 2000 | 11094043 |
| vitellogenin-induced pathology in male summer flounder (paralichthys dentatus). | male summer flounder (paralichthys dentatus) were given two injections (initially and 2 weeks later) of 17beta-estradiol (e2) totaling 0.2 (2 x 0.1), 2.0 (2 x 1.0) or 20.0 (2 x 10.0) mg e2/kg body weight. blood and tissue samples were collected 4, 6 and 8 weeks after the initial injection in the (2 x 0.1) mg/kg treatment, 4, 6, 8, and 15 weeks after the first injection in the (2 x 1.0) mg/kg treatment and at 4 weeks only in the (2 x 10.0) mg/kg treatment. five of the 12 fish injected twice with ... | 2001 | 11090901 |
| collagen-affecting drugs impair regeneration of teleost tail fins. | regenerating tail fins were studied in two species of teleosts, tilapia rendalli and cyprinus carpio, treated with indomethacin, aspirin, dexamethasone, penicillamine, and beta-aminoproprionitrile, drugs known to disrupt collagen metabolism in mammals. collagen was studied under the light microscope by the picrosirius-polarization method and also under the electron microscope. in general, these drugs disturbed the deposition and organization of collagen fibrils leading to abnormally thin or prac ... | 2000 | 11085216 |
| molecular cloning of carp (cyprinus carpio) leucocyte cell-derived chemotaxin 2, glia maturation factor beta, cd45 and lysozyme c by use of suppression subtractive hybridisation. | 2000 | 11081442 | |
| genetic evidence for gonochoristic reproduction in gynogenetic silver crucian carp (carassius auratus gibelio bloch) as revealed by rapd assays. | sex evolution has been a debating focus in evolutionary genetics. in lower vertebrates of reptiles, amphibians, and fish, a species or a bioform reproduces either sexually or asexually but never both. a few species were found to consist of all females in fish. these all-female species can propagate by asexual reproduction modes, such as gynogenesis and hybridogenesis. however, the coexistence of sexuality and asexuality in a single species was recently noted only in a cyprinid fish silver crucia ... | 2000 | 11080373 |
| light-adaptive role of nitric oxide in the outer retina of lower vertebrates: a brief review. | the role of nitric oxide (no) as a novel neurochemical mechanism controlling light adaptation of the outer retina is discussed by considering mainly published results. the emphasis is on the retinae of fishes and amphibia, but some data from the mammalian (rabbit) retinae have also been included for completeness. in the fish retina, application of no donors in the dark caused light-adaptive photomechanical movements of cones. the normal effect of light adaptation in inducing cone contractions wa ... | 2000 | 11079398 |
| effects of environmental temperature on the development of the myotomal white muscle in larval carp (cyprinus carpio l.). | a study was conducted on common carp (cyprinus carpio l.) to determine the effects of environmental temperature experienced by embryos and larvae on the development of myotomal white muscle. eggs from one female were divided into two groups following fertilisation and incubated at constant pre-hatch temperatures of 18 or 28 degrees c. at hatching, larvae from the 18 degrees c-incubated eggs were divided into two groups and either reared at the same temperature of 18 degrees c ('cold' group) or t ... | 2000 | 11076732 |
| determination of 4-nonylphenol, nonylphenol monoethoxylate, nonylphenol diethoxylate and other alkylphenols in fish and shellfish by high-performance liquid chromatography with fluorescence detection. | a simple and highly sensitive method is described for the hplc determination of 4-nonylphenol (np), 4-nonylphenol mono- (np1eo) and diethoxylates (np2eo) in fish and shellfish together with bisphenol a (bpa), 4-tert.-butylphenol (bp) and 4-tert.-octylphenol (op). the np, np1eo, np2eo and other alkylphenols in the samples are extracted with acetonitrile and the lipid in the sample extract is eliminated by partitioning between hexane and acetonitrile. after florisil pr clean-up the sample extract ... | 2000 | 11076083 |
| horseradish peroxidase binding to intestinal brush-border membranes of cyprinus carpio. identification of a putative receptor. | morphologic studies have shown that the classic endocytosis tracer horseradish peroxidase (hrp) is actively internalized by vesicular transport in the carp intestine, suggesting the existence of specific binding sites in the apical membrane of enterocytes. the aim of the present study was to develop an in vitro binding assay using isolated carp intestinal brush-border membranes (bbm) to demonstrate and characterize these specific hrp binding sites. the results obtained show that hrp binding to b ... | 2000 | 11074599 |
| environmental acclimatization of the carp modulates the transcription of beta-actin. | a cascade of mechanisms involving changes in gene expression are substantial to shape the adaptive responses that a eurythermal fish requires upon environmental changes in its habitat. we have previously shown that the cyclical reprogramming of rrna transcription is a remarkable feature in carp under seasonal acclimatization. using in situ hybridization and competitive rt-pcr we found significant differences in beta-actin transcripts, generally accepted to be coded by a typical housekeeping gene ... | 2000 | 11074593 |
| genomic organization and the promoter region of the round-spotted pufferfish (tetraodon fluviatilis) cdc37 gene. | the cdc37 gene was isolated from a round-spotted pufferfish genomic library and characterized. this gene is composed of nine exons spanning 3.5 kb. exon 1 contains the 5'-untranslated region and exon 2 contains the putative translation initiation site. by 5'-race (rapid amplication of cdna ends) and sequence analysis, we deduced the promoter region for the cdc37 gene and found that it does not contain typical tata or ccaat box. the 1.8 kb dna fragment upstream of the putative transcription initi ... | 2000 | 11072077 |
| multiple lineages of the non-ltr retrotransposon rex1 with varying success in invading fish genomes. | rex1, together with the related babar: elements, represents a new family of non-long-terminal-repeat (non-ltr) retrotransposons from fish, which might be related to the cr1 clade of line elements. rex1/babar: retrotransposons encode a reverse transcriptase and an apurinic/apyrimidinic endonuclease, which is very frequently removed by incomplete reverse transcription. different rex1 elements show a conserved terminal 3' untranslated region followed by oligonucleotide tandem repeats of variable si ... | 2000 | 11070055 |
| molecular and functional diversity of neural connexins in the retina. | electrical synapses (gap junctions) in neuronal circuits have become a major focus in the study of network properties such as synchronization and oscillation (galarreta and hestrin, 1999; gibson et al., 1999). despite the recent progress made in unraveling the contribution of gap junctions to network behavior, little is known about the molecular composition of the junctional constituents. by cloning gap junction proteins [connexins (cxs)] from zebrafish retina and through functional expression, ... | 2000 | 11069940 |
| evaluation of the genotoxic and cytochrome p450 monooxygenase-inhibitory potential of dicuran on procaryotic and eucaryotic test systems. | the effect of the herbicide dicuran 500 fl (formulated product) on the phenotypical and genotypical changes in procaryotic and eucaryotic organisms was investigated using short-term tests for detecting genotoxins. since pesticides discharged in the water environment can modulate the mixed-function monooxygenases (mfo) detoxification system of water organisms, the in vivo and in vitro effects of dicuran on hepatic cytochrome p450 (cyt p450) monooxygenase activities were also examined in juvenile ... | 2000 | 11069017 |
| characterization of parameters for in vitro culture of isolated ovarian follicles of greenback flounder rhombosolea tapirina. | isolated ovarian follicles of greenback flounder rhombosolea tapirina were incubated with a variety of gonadotropins (gths) and steroid precursors for periods of up to 42 h, and levels of free and glucuronated testosterone (t) and 17beta-estradiol (e(2)) in the medium, and free t and e(2) from inside follicles were measured by ria. short incubations (6 h) generated increases in t and e(2) in response to steroid precursors, but not human chorionic gth (hcg), or salmon or carp gth. at incubation t ... | 2000 | 11064285 |
| cyclic amp-receptor proteins in the enamel matrix. | cyclic amp receptor proteins (carp) are present in a variety of cell types. intracellularly, they are the regulatory (r) subunits of type ii cyclic amp-dependent protein kinase (pka: e.c.2.7.1.37). additionally, carp are secretory products of several cell types.[1] that carp are present in and secreted by ameloblasts into the enamel matrix of the rat incisor was demonstrated by photoaffinity labeling, western blotting and immunogold cytochemistry. gold particles were present over cytoplasmic reg ... | 1998 | 11063036 |
| cloning, sequencing, and analysis of expression of a second il-1beta gene in rainbow trout (oncorhynchus mykiss). | the full-length sequence of a second il-1beta gene (il-1beta2) in rainbow trout (oncorhynchus mykiss) has been obtained. as with the first il-1beta gene, il-1beta2 is organized into six exons/five introns. there are only small differences in their intron/exon sizes, with the exception of intron 3, which is 334 bp smaller in il-1beta2. the transcript encoded by the il-1beta2 gene contains a 5' untranslated region (utr) of 121 bp, followed by a 762-bp open reading frame and a 518-bp 3'utr. the 3'u ... | 2000 | 11061285 |
| concentrations of some heavy metals in water, sediment and fish species from the atatürk dam lake (euphrates), turkey. | concentrations of heavy metals (cd, co, cu, fe, hg, mn, mo, ni, pb and zn) were measured in the water, sediment and fish species (acanthobrama marmid, chalcalburnus mossulensis, chondrostoma regium, carasobarbus luteus, capoetta trutta and cyprinus carpio) from the atatürk dam lake, turkey. among the heavy metals studied cd, co, hg, mo and pb were not detected in water, sediments and fish samples, while ni was undetectable levels in fish samples. levels of cu, fe, mn and zn varied depending on d ... | 2000 | 11057573 |
| early structural changes in myosin rod upon heating of carp myofibrils. | upon heating carp myofibrils at 40 degrees c, the amount of myosin that is soluble and monomeric dropped very quickly, roughly 5 times faster than the atpase inactivation. this rapid decrease of solubility was well explained by a rapid denaturation of the rod portion as measured by chymotryptic digestibility. chymotryptic digestion of heated myofibrils in a low-salt medium with edta generated a reduced amount of rod and subfragment-1 (s-1). the decrease of s-1 produced from the heated myofibrils ... | 2000 | 11052753 |
| in vitro effects of beta-hydroxy-beta-methylbutyrate (hmb) on cell-mediated immunity in fish. | beta-hydroxy-beta-methyl butyrate(hmb) has been shown to counteract many of the negative effects of intensive animal production methods and results in increased growth and protection against diseases. in the present study, the effect of hmb on the immunocompetence cell activity in rainbow trout (oncorhynchus mykiss) and carp (cyprinus carpio) was examined. pronephric phagocytes and lymphocytes were isolated from the fish and grown in culture medium (rpmi-1640) containing either 0, 0.1, 1, 5, 10, ... | 2000 | 11044553 |
| a case with 47,xxy,del(11)(q23) karotype-coexistence of jacobsen and klinefelter syndromes. | a case with 47,xxy, del(11)(q23) karyotype-coexistence of jacobsen and klinefelter syndromes: a two-year-old dysmorphic male child was found to have 47,xxy,del(11)(q23) karyotype. domination of the clinical features of jacobsen syndrome was observed: mild mental retardation, trigonocephaly, ptosis, downward slanting palpebral fissures, low set ears, carp-shape mouth and micrognathia. transient thrombocytopenia and leukopenia were also present. over the following five years gynecomastia and eunuc ... | 2000 | 11043435 |
| assessment of water quality in wichita, kansas, using an index of biotic integrity and analysis of bed sediment and fish tissue for organochlorine insecticides. | this study investigated water quality of the arkansas river and its tributaries in the wichita, kansas, area. water quality assessment included an evaluation of urban versus agricultural areas using a twofold approach: (1) assessment of fish community health using an index of biotic integrity (ibi); and (2) analysis of organochlorine (oc) insecticides in fish tissue (cyprinus carpio) and sediment samples via gas chromatography. results of the ibi analysis indicated that the overall health of the ... | 2000 | 11031315 |
| invertebrate and fish cytokines. | cytokine-like molecules are well described in invertebrates, although most recent studies have revealed that there is analogy, rather than homology, between invertebrate and vertebrate cytokine-like activities. cytokines certainly appeared early in the evolution of vertebrates, dating back some 400 millions years. here, evidence will be reviewed and updated of the presence of these molecules in jawed fish and in particular, in bony fish, which represent the oldest group displaying true functiona ... | 2000 | 11022118 |
| binding of xenobiotics to hepatic estrogen receptor and plasma sex steroid binding protein in the teleost fish, the common carp (cyprinus carpio). | competitive receptor binding assays have been suggested as an in vitro screening tool for assessing the activity of alleged estrogenic substances. in this study, we determined the ability of steroidal and nonsteroidal substances to inhibit the binding of [(3)h]17 beta-estradiol (e2) to the hepatic estrogen receptor (er) and the plasma sex steroid binding protein (sbp) of the teleost fish, the common carp (cyprinus carpio). the objectives of the study were (1) to characterize er binding in the li ... | 2000 | 11017776 |
| color-opponent responses of small and giant bipolar cells in the carp retina. | the physiological and morphological properties of color-opponent bipolar cells in the carp retina were studied. fifty nine off-center bipolar cells and 63 on-center bipolar cells out of about 500 total bipolar cells recorded showed color-opponent responses. the off-center color-opponent bipolar cells were classified into three subgroups according to their spectral and spatial responses. fifty off-center color-opponent cells responded with depolarization to a blue light spot and with hyperpolariz ... | 2000 | 11016579 |
| endocrine disruption by hexachlorobenzene in crucian carp (carassius auratus gibelio). | 2000 | 11014838 | |
| the homologue of mannose-binding lectin in the carp family cyprinidae is expressed at high level in spleen, and the deduced primary structure predicts affinity for galactose. | mannose-binding lectin (mbl) participates in the innate immune system as an activator of the complement system and as an opsonin after binding to certain carbohydrate structures on microorganisms. we isolated and characterized cdna transcripts encoding an mbl homologue from three members of the carp family cyprinidae, the zebrafish danio rerio, the goldfish carassius auratus, and the carp cyprinus carpio. the carp and zebrafish transcripts contain two polyadenylation sites and rt-pcr on mrna fro ... | 2000 | 11003389 |