Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| accessory cell function of cells of the mononuclear phagocyte system isolated from mycobacterial granulomas. | epithelioid cells from bcg-induced granulomas and macrophages from mycobacterium leprae-induced granulomas were examined for their ability to act as accessory cells for t-cell proliferation to mitogen (con a) and antigen (ppd). the granuloma cells were separated on a facs using monoclonal antibody specific to guinea pig macrophages. epithelioid cells (which are ia negative) were able to support proliferation to con a but not to antigen. cultures containing ia positive granuloma macrophages from ... | 1985 | 3888411 |
| the search for new drugs for the treatment of leprosy. | 1985 | 3887081 | |
| mycobacterium leprae in seminal fluid: a case report. | 1985 | 3887080 | |
| leprosy and the eye: a review. | 1985 | 3886906 | |
| [diagnostic test systems based on the immunoenzyme method in leprosy]. | diagnostic test systems for the detection of igg and igm to mycobacterium leprae in the blood sera of leprosy patients and armadillos experimentally infected with m. leprae have been developed on the basis of the indirect immunoperoxidase assay. the possibility has been shown of prognosing the activity of the leprotic process in leprosy patients and the results of the experimental infection of armadillos by the dynamic increase of antibody reactions with the development of the infection. | 1985 | 3885647 |
| molecular analysis of dna and construction of genomic libraries of mycobacterium leprae. | molecular analysis of dna from mycobacterium leprae, "mycobacterium lufu," and mycobacterium vaccae has demonstrated that the g + c (guanine plus cytosine) contents of the dnas are 56, 61, and 65%, respectively, and that the genome sizes are 2.2 x 10(9), 3.1 x 10(9), and 3.1 x 10(9) daltons, respectively. because of the significant differences in both g + c content and genome size among m. leprae, "m. lufu," and m. vaccae dnas, these species are not related, although hybridization experiments un ... | 1985 | 3882664 |
| manifestations of experimental leprosy in the armadillo. | three experiments, using different routes and doses of infection, were conducted using 42 armadillos. thirty-six of them developed generalized disease. there is no significant sex or age difference in susceptibility. route and dose of infection make very little difference in the disease prevalence except that the intravenous administration of a large dose reduces the period of development of generalized disease. it is quite possible that in armadillos the resistance to the disease is partly gene ... | 1985 | 3882008 |
| susceptibility of mycobacterium leprae to the bactericidal activity of mouse peritoneal macrophages and to hydrogen peroxide. | macrophages from athymic nude mice were infected in vitro with mycobacterium leprae to study the intracellular fate of this organism. using the proportional bactericidal test, we have shown that the viability of m. leprae declines rapidly within these macrophages, although results of clearance experiments demonstrate that live and killed organisms are cleared at comparable rates. we have also shown that m. leprae is susceptible to the bactericidal effects of hydrogen peroxide and we suggest that ... | 1985 | 3881593 |
| hla-linked control of predisposition to lepromatous leprosy. | in a study of the relation between hla and lepromatous leprosy, hla haplotype segregation was analyzed in 28 families with multiple cases of different types of leprosy. the inheritance of hla-dr2, hla-dr3, and hla-mt1, which had previously been shown to be associated with susceptibility to leprosy or with a leprosy type, was analyzed separately. segregation occurred in a significantly nonrandom fashion in both polar tuberculoid leprosy and lepromatous leprosy. this finding indicated hla-encoded ... | 1985 | 3880800 |
| adoptive cell transfer of resistance to mycobacterium leprae infections in mice. | cells were transferred from mice intradermally vaccinated with killed mycobacterium leprae to sublethally irradiated recipients. unseparated cells from lymph nodes or spleens of m. leprae vaccinated mice were found to cause significant inhibition of the growth of a subsequent m. leprae challenge in mouse footpads for up to 26 weeks after vaccination. vaccination with live bcg and cells transferred from bcg-vaccinated mice caused no significant inhibition of m. leprae growth in mouse footpads. ce ... | 1985 | 3876183 |
| lepromatous placentitis and intrauterine fetal infection in lepromatous nine-banded armadillos (dasypus novemcinctus). | three pregnant lepromatous armadillos along with the three sets of four fetuses and their placentae were studied histopathologically. mycobacterium leprae were present in the decidual tissue, trophoblastic cells which line the chorionic villi, and in the cells that form the internal structure of the villi. acid-fast organisms were also seen in the spleens of three fetuses. congenital infection is clearly possible in leprosy in the armadillo and may also occur in humans. | 1987 | 3795871 |
| the lepromin test in rhesus monkeys. | the lepromin test was studied in rhesus monkeys. six control monkeys which had not been inoculated with mycobacterium leprae, six monkeys with experimentally induced leprosy, and nine monkeys which had been inoculated with m. leprae but had not developed leprosy were evaluated with 1x, 10x, and 15x lepromin a, with 1x and 10x lepromin m (mangabey monkey derived), with 1x and 25x purified inactivated m. leprae, and with an armadillo mock lepromin. we found that the lepromin test is useful in rhes ... | 1986 | 3746044 |
| ganglioside patterns in normal and lepromatous armadillo tissues. | gangliosides derived from tissues of normal and lepromatous armadillos were examined by thin-layer chromatography. the ganglioside patterns produced by the mycobacterium leprae-infected tissues varied from that of the normal tissue. although increased levels of several gangliosides were observed in the infected tissues, there was an apparent preferential increase in gm3 gangliosides as determined by column chromatography and enzymatic hydrolysis. | 1986 | 3722965 |
| hepatitis b virus (hbv) serum markers in greek leprosy patients. | the prevalence of hbsag, anti-hbs, and anti-hbc in the sera of 217 patients with the two polar types of leprosy and 382 hospital controls was studied in order to investigate the degree of exposure of greek leprosy patients to hbv and the ability of these patients to clear hbv from the blood. two distinct serological patterns were analyzed: effective exposure, characterized by the presence of one or more of the three serological markers, and active infection, characterized by the presence of hbsa ... | 1986 | 3722963 |
| effective prophylactic measures against accidental inoculation with live mycobacterium leprae in laboratory and hospital workers. | 1987 | 3669870 | |
| biochemical characteristics and fatty acid compositions of some armadillo-derived mycobacteria and their relation to mycobacterium gordonae. | the long-chain components of 75 strains of mycobacteria, cultivated from mycobacterium leprae-infected or non-infected armadillos, and of eight clinical and 15 environmental isolates of m. gordonae, were compared. four major groups could be distinguished based on the presence of 10-methyloctadecanoic (tuberculostearic) and 2-methyl 3-hydroxyeicosanoic acids and secondary alcohols (2-octadecanol and 2-eicosanol). some heterogeneity was found in strains assigned to m. gordonae: the characteristic ... | 1987 | 3655731 |
| social and demographic aspects of a leprosy epidemic on a polynesian atoll: implications of pattern. | this study reports the results of field research on a leprosy epidemic among the kapingamarangi people, polynesians living in two communities on ponape island and kapingamarangi atoll in the federated states of micronesia. the patterns of infection in the two communities are seen to replicate in detail patterns of personal mobility by age and gender and patterns of kinship and friendship relations that order people's social interactions in the communities. these patterns of demographic and socia ... | 1987 | 3655462 |
| an investigation of family size and birth order as risk factors in leprosy. | to investigate if early exposure to mycobacterium leprae is a factor determining development of hansen's disease in general, or of a particular form of the disease, we have studied the sibship size and birth order distribution of 187 leprosy cases and 528 hospitalized control patients. by the greenwood-yule analysis, a small deficit of cases in the earlier birth order in the tuberculoid form was observed. such a deficit was not observed in lepromatous leprosy patients. in the multivariate analys ... | 1987 | 3655461 |
| macrophage chemotaxis in mycobacterium leprae infected mice. | 1987 | 3596680 | |
| development of a dot-elisa for detection of leprosy antigenuria under field conditions. | 1986 | 3573947 | |
| simplification and standardization of serodiagnostic tests for leprosy based on phenolic glycolipid-i (pg-i) antigen. | 1986 | 3573946 | |
| structure of mycobacterium leprae. | 1986 | 3573941 | |
| is phenolic glycolipid-1 specific for mycobacterium leprae? | 1986 | 3572110 | |
| circulating immune complexes in normal and immunosuppressed mice infected with mycobacterium leprae. | mycobacterium leprae infection was produced through the footpads in normal and immunosuppressed mice. circulating immune complexes were detected by specific binding test and by conglutinin binding assay for specific and total immune complexes respectively in the sera of these mice during different periods of infection. out of the total 30 samples tested from the infected groups, 3 were positive by specific binding test and 5 by conglutinin binding elisa. the implications of the findings in relat ... | 1986 | 3572093 |
| experimental leprosy in a rhesus monkey: necropsy findings. | a 6-month-old male rhesus monkey (macaca mulatta) was inoculated intravenously and intracutaneously with mycobacterium leprae obtained from a naturally infected mangabey monkey. the animal developed generalized lepromatous leprosy, and was killed for pathological examination 56 months after inoculation. lesions were observed in the skin, nasal mucosa, peripheral nerves, and peripheral lymph nodes, with relative sparing of viscera. the monkey was carefully evaluated for the retrovirus stlv-iii in ... | 1987 | 3559334 |
| investigations into the cultivation of mycobacterium leprae. a multifactorial approach. | 1986 | 3553813 | |
| isolation of environment-derived mycobacterium leprae from soil in bombay. | 1986 | 3553812 | |
| host-pathogen interaction--new in vitro drug test systems against mycobacterium leprae--possibilities and limitations. | 1986 | 3553811 | |
| metabolism in mycobacterium leprae: possible targets for drug action. | 1986 | 3553810 | |
| single bacterial cell mass analysis: a rapid test method in leprosy therapy control. | 1986 | 3553809 | |
| limited in vitro multiplication of mycobacterium leprae: application to screening potential antileprosy compounds. | 1986 | 3553808 | |
| the use of rodent models in assessing antimicrobial activity against mycobacterium leprae. | 1986 | 3553807 | |
| characterization of the 36 k antigen of mycobacterium leprae. | 1986 | 3553806 | |
| the characterization and immunoreactivity of a 70 kd protein common to mycobacterium leprae and mycobacterium bovis (bcg). | 1986 | 3553805 | |
| the carbohydrate-containing antigens of mycobacterium leprae. | 1986 | 3553804 | |
| murine t-cell reactivity to cloned mycobacterium leprae antigens. | 1986 | 3553803 | |
| humoral immune responses to m. leprae in human volunteers vaccinated with killed, armadillo-derived m. leprae. | 1986 | 3553802 | |
| the karonga prevention trial--which bcg? | 1986 | 3553801 | |
| the development of a vaccination model using two microorganisms and its application in leprosy and leishmaniasis. | 1986 | 3553799 | |
| cloning of mycobacterium leprae genes in streptomyces. | 1986 | 3553797 | |
| anti-mycobacterial antibodies in saliva. | 1986 | 3553796 | |
| the specificity of the immunodeficiency in lepromatous leprosy. | 1986 | 3553795 | |
| the contribution of charles c. shepard to leprosy research: from the mouse footpad model to new dna technology. | 1986 | 3553793 | |
| obligate intracellular parasites do not synthesize atp. | 1986 | 3553365 | |
| bacillaemia in smear negative leprosy. | 1986 | 3553364 | |
| on the "culture medium for cultivation of m. leprae" published by dr. laszlo kato. | 1986 | 3553363 | |
| multiple relapses in borderline leprosy--a case report. | a case of borderline lepromatous leprosy with a history of 5 years duration of disease, was first seen in 1971 and treated with graded doses of dapsone in the op clinic of the institute. he became inactive and bacteriologically negative in 3 years. while continuing on dapsone therapy he relapsed into active dapsone resistant leprosy 3 1/2 years later. he was admitted in the hospital and given rifampicin 600 mg. daily for 15 days along with dapsone 100 mg. daily. he again became inactive and bact ... | 1986 | 3553362 |
| a comparative study of the efficacy of who and ial multidrug therapy regimens for leprosy--an in-vivo and in-vitro study. | in the absence of definite evidence on utility of intensive therapy with rifampicin in multibacillary leprosy cases, a laboratory based investigation was undertaken basically to compare the efficacy of who and ial regimens. in each group 4 untreated bl-ll patients were included and their skin biopsies were subjected for viability test both in vitro and in vivo systems. a consistant fall in bi with good clinical improvement was observed in both the groups. however good viability was maintained ti ... | 1986 | 3553361 |
| cellular and humoral immune response to a phenolic glycolipid antigen (phengl-i) in patients with leprosy. | the ability of phenolic glycolipid i (phengl-i) of mycobacterium leprae to stimulate in vitro lymphocyte proliferation (lp) was tested in cultures of peripheral blood cells from 42 patients with leprosy in chicago and thailand, 9 individuals with household contact in thailand, and 10 unexposed north american controls. only 10 responders (24%) were found among the patients, and the degree of lp was small. responders were found among patients with lepromatous (18%) or tuberculoid (30%) leprosy wit ... | 1987 | 3553227 |
| metabolism of mycobacterium leprae in macrophages. | the incorporation of 14c-labeled palmitic acid ( [u-14c]pa) into the phenolic glycolipid-i (pgl-i) fraction of mycobacterium leprae was studied in a murine macrophage system in vitro. peritoneal macrophages from swiss webster mice were infected with fresh viable or formalin-killed m. leprae harvested from infected footpads of nu/nu mice, and [u-14c]pa was added to the culture medium. labeled glycolipid synthesized by live m. leprae was fractionated on a florisil-silicic acid column and identifie ... | 1987 | 3552993 |
| [antibacterial chemotherapy in leprosy: principles, objectives and recommendations]. | 1986 | 3551474 | |
| the "hansen-anergic fringe". | the hansen-anergic fringe ("haf") of the population is the minority genetically incapable of developing that variety of specific immunity to myco. hansenii which leads to the positivity of the mitsuda reaction. in spite of the fact that the haf was identified as early as 1937, its real proportion in the human species is still not exactly known. considering that myco. tuberculosis has been proved to induce mitsuda-positivity in the genetically capable majority, studies with the tuberculin reactio ... | 1986 | 3551473 |
| [immunogenetics of leprosy]. | 1986 | 3551470 | |
| [inadequate rifampicin treatment in multibacillary leprosy and the incubation time of relapse]. | 1986 | 3551469 | |
| [dynamics of establishing resistance of m. leprae to dapsone and rifampicin in martinique]. | 1986 | 3551468 | |
| [vaccination against leprosy]. | 1986 | 3551467 | |
| [drug resistance of mycobacterium leprae]. | 1986 | 3551466 | |
| events surrounding the recognition of mycobacterium leprae in nerves. | histological examination and immunocytochemistry of schwann cells, macrophages, and mycobacterial antigen were used to study 48 nerves of untreated patients with leprosy. none of the patients was in reaction clinically, but microreactions, involving small clusters of schwann cells and macrophages in all cases except ll, were marked by progressive degradation of acid-fast bacilli (afb). this was thought to be the response to the recognition of mycobacterial antigen. in the first phase, the disint ... | 1987 | 3549942 |
| further studies of the killing of m. leprae by aminoglycosides: reduced dosage and frequency of administration. | the bactericidal activity of the aminoglycoside antibiotics streptomycin and kanamycin for mycobacterium leprae in mice was assessed, both alone and in combination with rifampin, utilizing various dosage schedules. as in previous studies, 100 mg/kg five times weekly of streptomycin and kanamycin resulted, respectively, in 96% +/- 2% and 89% +/- 6% bactericide. reducing the dosage of streptomycin to 50 mg/kg, 25 mg/kg, and even 12.5 mg/kg resulted in less but significant bactericidal activity. su ... | 1987 | 3549941 |
| activities of pefloxacin and ciprofloxacin against mycobacterium leprae in the mouse. | because ciprofloxacin and pefloxacin are fluoroquinolones active against many mycobacterial species, both drugs were tested against mycobacterium leprae in the mouse foot-pad system. preliminary pharmacokinetic studies in the mouse showed that after a single oral dose of 150 mg/kg ciprofloxacin the peak serum concentration was 3.6 micrograms/ml, and after 50 mg/kg or 150 mg/kg pefloxacin peak serum concentrations were, respectively, 9.2 micrograms/ml and 16.9 micrograms/ml, the half-lives for se ... | 1987 | 3549940 |
| enhanced cell-mediated immune responses in erythema nodosum leprosum reactions of leprosy. | cell-mediated immune (cmi) responses were measured in 39 lepromatous, 44 erythema nodosum leprosum (enl), and 22 post-enl patients. the leukocyte migration inhibition test was used to measure cmi responses to mitogen phytohemagglutinin-p (pha), crossreacting antigen purified protein derivative (ppd) of tuberculin, and armadillo-derived whole and sonicate mycobacterium leprae. "early t" lymphocytes of the peripheral blood were also enumerated using the rosetting technique. significantly enhanced ... | 1987 | 3549939 |
| in vitro proliferation of lymphocytes from human volunteers vaccinated with armadillo-derived, killed m. leprae. | a killed, armadillo-derived mycobacterium leprae vaccine was examined for its ability to induce cell-mediated responsiveness in purified protein derivative (ppd)-positive volunteers residing in a nonendemic country using the lymphocyte transformation test (ltt). a marked increase in the proliferative responses to a m. leprae-soluble antigen preparation was observed in the two groups which were vaccinated with the highest doses of the vaccine, i.e., 1.5 x 10(8) and 5 x 10(8) bacilli. this increas ... | 1987 | 3549938 |
| genetic epidemiology of the susceptibility to leprosy. | to test the hypothesis that genetic factors are operative in the predisposition to leprosy (hansen's disease) in humans, a genetic epidemiologic investigation was performed on 269 leprosy kindreds containing 552 affected individuals from an isolated population in papua new guinea. the community, and not the family, was the basic social unit. leprosy, an infectious disease, was not communal but strongly familial within the karimui. segregation analysis, to determine whether a major gene for the s ... | 1987 | 3549780 |
| analysis of monoclonal antibodies to mycobacterium leprae by crossed immunoelectrophoresis. | monoclonal antibodies to mycobacterium leprae were characterized in crossed immunoelectrophoresis and showed markedly different patterns of reactivity with m. leprae lines 2, 7 and 11 respectively. line 7 corresponds to a cell wall-associated macromolecular complex containing lipid, polysaccharide, and two distinct 36 k and 65 k proteins. | 1987 | 3547616 |
| host-parasite interrelationship between m. leprae and schwann cells in vitro. | 1986 | 3546550 | |
| classification of nerves is modified by the delayed recognition of mycobacterium leprae. | biopsies of 42 concurrent nerve and skin lesions across the spectrum of leprosy were classified and compared histologically and bacteriologically. observations were made as follows: a) the bacterial load was higher in nerve than in skin lesions of the same histological classification, and it was higher in nerve than in concurrent skin lesions irrespective of classification, although not at the lepromatous pole. b) there was some discrepancy between the histological classification of nerve and sk ... | 1986 | 3546549 |
| antimycobacterial activities of two newer ansamycins, r-76-1 and dl 473. | the antimycobacterial activities of two newer ansamycins, isobutylpiperazinylrifamycin sv (r-76-1) and cyclopentylrifamycin sv (dl 473), were compared with those of rifampin (rmp) both in vitro and in vivo. in terms of minimal inhibitory concentrations against a number of cultivable mycobacteria, r-76-1 was about eight times more active in vitro than rmp; whereas dl 473 was only slightly more active than rmp. therapeutic activities of r-76-1 versus rmp and dl 473 versus rmp were compared, respec ... | 1986 | 3546547 |
| in vitro and in vivo activities of ofloxacin against mycobacterium leprae infection induced in mice. | ofloxacin, a new quinolone, exhibits bactericidal activity against mycobacterium leprae in mice, both in vivo and in vitro. | 1986 | 3546546 |
| evaluation of monitoring antibodies to pgl-i in armadillos experimentally infected with m. leprae. | an enzyme-linked immunosorbent assay (elisa) for igm antibodies to the phenolic glycolipid-i antigen of mycobacterium leprae was evaluated for efficacy in monitoring armadillos experimentally infected with the bacillus. igm antibodies were detected in armadillos from 186 days after experimental infection until the animals were sacrificed. the elisa demonstrated the establishment of infection earlier and more reliably than the histologic methods previously applied. satisfactory yields of m. lepra ... | 1986 | 3546545 |
| selection of sites for slit skin smears in untreated and treated leprosy patients. | a total of 1000 slit-skin smears were taken from multiple sites (both ear lobules, right elbow, dorsal aspect of middle phalanx of the left finger, and dorsum of middle phalanx of the right foot) from 558 leprosy patients. there were 319 sets (1595 smears) from untreated patients and 681 sets (3405 smears) from the treated group. the duration of treatment varied from 6 months (multidrug therapy) to 7 years (dapsone monotherapy). the ear lobules gave significantly higher values for the bacterial ... | 1986 | 3546544 |
| elisa detection of igm antibodies against phenolic glycolipid-i in the management of leprosy: a comparison between laboratories. | igm antibodies to the phenolic glycolipid-i (pgl-i) antigen of mycobacterium leprae were detected by different elisa techniques in three laboratories (in new york, colorado, seattle, u.s.a.). the agreement on seropositivity and overall correlation between techniques was excellent. a positive linear correlation between the bacterial index (bi) and anti-pgl-i igm, previously reported by the new york laboratory, was detected by all techniques. the role of erythema nodosum leprosum in decreasing the ... | 1986 | 3546543 |
| inhibition of the interaction of mycobacteria with schwann cells by antimycobacterial antibodies. | the effect of antimycobacterial serum on the uptake of mycobacterium w by 33b rat schwannoma cell line and rat peritoneal macrophages was studied. incubation of mycobacterium w with antimycobacterial serum caused a marked inhibition of its uptake by 33b cells but did not diminish its uptake by macrophages. these results suggest that the mechanism of the interaction of mycobacteria with schwann cells differs from that with macrophages. | 1987 | 3546373 |
| intracellular fate of mycobacterium leprae in normal and activated mouse macrophages. | mycobacterium leprae replicates within mononuclear phagocytes, reaching enormous numbers in the macrophage-rich granulomas of lepromatous leprosy. to examine the capability of macrophages to digest m. leprae, we studied the intracellular fate of m. leprae organisms in normal and activated mouse macrophages by using the electron-dense secondary lysosome tracer thoria sol. intracellular m. leprae organisms, surrounded by a characteristic electron-transparent zone, were contained within phagosomal ... | 1987 | 3546136 |
| methods for the detection of a specific mycobacterium leprae antigen in the urine of leprosy patients. | two methods for detecting the phenolic glycolipid, pgl-1, a mycobacterium leprae-specific molecule, in the urine of leprosy patients are described. both methods rely on the 100-fold preconcentration of the urine, which can be accomplished by a single-step ultrafiltration procedure. the equivalent of approximately 2.5 micrograms of pgl-1/ml was detected in the urine of ll patients with an inhibition elisa. the second method, a direct dot-blot assay on nitrocellulose paper, was much simpler and mo ... | 1987 | 3544198 |
| demonstration of leprosy bacilli in the eyes of experimentally infected armadillos: a comparison of five melanin bleaching methods. | 1986 | 3543588 | |
| a dot enzyme immunoassay for detection of igm antibodies against phenolic glycolipid-i in sera from leprosy patients. | a visual dipstick dot enzyme immunoassay (eia) for diagnosis of leprosy is described. the assay is based on detection of igm antibodies against phenolic glycolipid (pgl-i) in sera from leprosy patients. the antigen (pgl-i or synthetic disaccharide of pgl-i) was dotted on a nitrocellulose pad stuck on a plastic strip (dipstick). sera were used at a dilution of 1:200. peroxidase coupled mouse anti-human igm monoclonal antibodies were used as the conjugate. a positive test gave a blue dot against a ... | 1986 | 3543160 |
| identification of specific and cross-reactive antigens of leishmania donovani chagasi by human infection sera. | cloned leishmania donovani chagasi (ldc) promastigotes were analyzed by sds-page separation and immunoblotting with human infection sera. the patterns of antigen reactivity were compared by using sera from individuals with ldc, leishmania mexicana amazonensis (lma), trypanosoma cruzi, mycobacterium tuberculosis, or mycobacterium leprae infections. sera from individuals with these infections recognized ldc antigens in several m.w. ranges. reactivity was due to recognition of ldc molecules and not ... | 1987 | 3543131 |
| mycobacterial plasmids: screening and possible relationship to antibiotic resistance in mycobacterium avium/mycobacterium intracellulare. | 1986 | 3540539 | |
| the killing of mycobacterium leprae in mice by various dietary concentrations of dapsone and rifampicin. | 1986 | 3540497 | |
| survey for primary dapsone resistance in cuba. | 1986 | 3540496 | |
| antigens of mycobacterium leprae in urine during treatment of patients with lepromatous leprosy. | 1986 | 3540495 | |
| antimycobacterial antibodies in dasypus novemcinctus infected with mycobacterium leprae and their correlation with the serum levels of lactate dehydrogenase. | 1986 | 3540494 | |
| how might mycobacterium leprae enter the body? | 1986 | 3540493 | |
| phenolic glycolipid-i of mycobacterium leprae induces general suppression of in vitro concanavalin a responses unrelated to leprosy type. | using a costimulant assay, in vitro con a responses of patients across the leprosy spectrum were found to be markedly suppressed by phenolic glycolipid-i (pgl-i), a unique antigen of m. leprae. the degree of inducible suppression as well as the number of leprosy patients showing suppression of mitogenic responses was higher with pgl-i as compared with integral m. leprae (p less than 0.05 to less than 0.01). both untreated lepromatous (60%) as well as tuberculoid leprosy (67%) patients showed sig ... | 1987 | 3540178 |
| mycobacterium leprae: metabolically competent, but microbe-dependent; indications for in vitro cultivation. | 1986 | 3540144 | |
| adcc function in mycobacterium leprae inoculated normal and immunosuppressed mice. | normal and immunosuppressed mice were inoculated with mycobacterium leprae obtained from untreated lepromatous patients. besides monitoring the afb counts in the footpads at 3,6 and 9 months post inoculation, antibody dependent cellular cytotoxicity (adcc) function was studied. the adcc function seen to be largely unaltered in the m. leprae infected animals, comparable to the observation made in human leprosy. | 1986 | 3540143 |
| sciatic nerve in experimental leprosy. | swiss albino mice were inoculated with mycobacterium leprae obtained from untreated lepromatous patients. histopathological study of sciatic nerves showed no abnormality. however a few free acid fast bacilli (afb) were detected in the sciatic nerves taken from the inoculated limbs during the early stages of infection, suggesting the nerve-fibre route of travel as seen in humans in experimental leprosy, too. | 1986 | 3540142 |
| lipase activity in mycobacterium leprae--an indicator of metabolic function. | presence of lipase, in mycobacterium leprae obtained from human nodules and infected armadillo tissues, has been detected by demonstrating the ability of the bacteria to hydrolyze tributyrin. this capacity is expressed during incubation of the bacteria with the substrate and needs a source of carbon and other energy metabolites. the activity is blocked by anti m. leprae drug rifampicin. it is concluded that expression of lipase activity is a metabolic event of m. leprae, while they are maintaine ... | 1986 | 3540141 |
| the cellular responses of tuberculosis and leprosy patients and of healthy controls in skin tests to 'new tuberculin' and leprosin a. | the density and distribution of t4 and t8 lymphocytes and of monocyte/macrophages at the site of skin tests with mycobacterial antigens was studied in pulmonary tuberculosis and leprosy patients and in healthy controls. most of the inflammatory cells were located in perivascular and periappendicular foci in the dermis: the percentage of the dermis occupied by focal infiltrate was unrelated to the clinical measurement of the area of induration. there was a less intense diffuse infiltrate in the d ... | 1986 | 3539419 |
| serodiagnosis of leprosy: relationships between antibodies to mycobacterium leprae phenolic glycolipid i and protein antigens. | sera from leprosy patients and controls were assayed for immunoglobulin m (igm) and igg antibodies to the mycobacterium leprae-specific phenolic glycolipid i antigen (pg) by enzyme-linked immunosorbent assay, for igg antibodies to m. leprae protein antigens by western immunoblot, and for antibodies to a 65-kilodalton (kda) protein antigen of m. leprae by a competition antibody binding assay. elevated levels of anti-pg igm were seen in lepromatous and borderline lepromatous patients, and elevated ... | 1986 | 3537003 |
| mpb59, a widely cross-reacting protein of mycobacterium bovis bcg. | the mpb59 protein of mycobacterium bovis bcg was purified to homogeneity from culture fluid of bcg substrain tokyo, and characterized by biochemical and immunological techniques. the molecular weight was 28,000, determined by sds-polyacrylamide gel electrophoresis, and the pi value was 5.3. the n-terminal amino acid sequence was determined for 32 steps and showed no significant homology with mpb64, mpb70 or mpb80. by crossed immunoelectrophoresis, mpb59 was found to belong to the bcg antigen 85 ... | 1986 | 3536756 |
| catalases, peroxidases, and superoxide dismutases in mycobacterium leprae and other mycobacteria studied by crossed immunoelectrophoresis and polyacrylamide gel electrophoresis. | the five mycobacteria mycobacterium lepraemurium, m. leprae, m. bovis bcg, m. smegmatis, and m. intracellulare were studied. catalase and peroxidase activities were demonstrated in polyacrylamide and crossed immunoelectrophoresis gels for m. lepraemurium, m. intracellulare, and bcg, but not for m. leprae. peroxidase and catalase activities were associated with the same precipitate line in crossed immunoelectrophoresis for m. lepraemurium, m. intracellulare, and bcg, showing that in these mycobac ... | 1986 | 3536745 |
| thorns in armadillo ears and noses and their role in the transmission of leprosy. | both ears from 494 wild nine-banded armadillos (dasypus novemcinctus) and nose specimens from 224 animals were collected and histopathologically studied. lepromatous granulomas were present in the ear specimens of ten of 494 animals. there were thorns in the ears of 22.5% of animals, and in 36.6% of the nose specimens. in one armadillo, there was evidence to suggest that mycobacterium leprae entered the tissue through the thorn pricks. in the normal habitat of the armadillo in louisiana there ar ... | 1986 | 3535729 |
| two potential improvements to bcg and their effect on skin test reactivity in the lebanon. | an account of an ongoing project to assess the possible benefits of two additives to bcg vaccine is presented. these additives are suspensions of irradiation killed mycobacterium vaccae in one case, and m. leprae in the other. groups of children aged 7-17 living in zgharta and akkar districts of north lebanon have received vaccination with bcg alone or with either of the two additives since 1980. this region was chosen since contact with environmental mycobacteria is small, but both leprosy and ... | 1986 | 3535188 |
| adherence of mycobacterium leprae to schwann cells in vitro. | adherence of mycobacterium leprae was studied in vitro in monolayer cultures of purified mouse schwann cells. the optimum temperature and time for adherence were determined. pretreatment of schwann cells with lipase reduced adherence, but pretreatment with trypsin enhanced it and with four monosaccharides--l-arabinose, l-galactose, l-rhamnose and d-glucose--there was no significant effect, indicating that the receptors involved in adherence may be lipid. | 1986 | 3534276 |
| naturally acquired leprosy in the nine-banded armadillo: a decade of experience 1975-1985. | a decade has passed since our first report of naturally acquired leprosy in the nine-banded armadillo. our studies and those of others during this period confirm the identification of the etiologic agent as mycobacterium leprae. confirmation is based on the results of histopathologic examination and microbiologic evaluations that included attempts to culture the organism, flourescent antibody studies, mycolic acid analysis, and dna determinations demonstrating complete relatedness between the na ... | 1986 | 3534127 |
| delayed-type hypersensitivity in human volunteers immunized with a candidate leprosy vaccine. | 1986 | 3533193 | |
| [hla and leprosy]. | although there is now accumulating evidence that the host response to mycobacterium leprae is genetically controlled, the nature of the genetic component is still imprecise. case-control studies as well as family studies, in various populations, have shown that hla linked factors confer susceptibility to tuberculoid leprosy and lepromatous leprosy respectively. recently, associations between gm allotypes and the disease have also been reported. further studies of the familial cosegregation of th ... | 1986 | 3531989 |
| a facile synthesis of 3,6-di-o-methyl-d-glucose. | 1986 | 3530456 | |
| immunoreactivity of a 70 kd protein purified from mycobacterium bovis bacillus calmette-guerin by monoclonal antibody affinity chromatography. | the protein antigens from mycobacterium bovis (bcg), m. tuberculosis, and m. leprae share a number of common determinants. we have used a murine mab (l7) recognizing such a determinant on a protein of mr 70,000 to purify this antigen from m. bovis sonicate by affinity chromatography. enrichment of the protein in column eluates was confirmed by immunoblotting and in antigen inhibition assays. after radiolabelling with 125i, the protein could be immunoprecipitated with human lepromatous leprosy se ... | 1986 | 3528377 |