Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| respiratory syncytial virus subgroup b dominance during one winter season between 1987 and 1992 in vancouver, canada. | a subgroup analysis of 613 specimens submitted to the british columbia's children's hospital from 1987 to 1992 revealed that subgroups a and b of respiratory syncytial virus (rsv) were both circulating in our community, with some predominance for subgroup a during the period from october 1987 to september 1988 (the 1987-88 season) (64%), 1990-91 (60%), and 1991-92 (62%). during 1989-90 subgroup a represented the majority of isolates (80%). subgroup b predominated during only one season, 1988-89 ... | 1994 | 8126189 |
| lower respiratory viral infections in immunocompetent children. | viral lower respiratory disease causes a heavy burden on our society. better understanding of the epidemiology of these viruses combined with new rapid diagnostic techniques will provide more rapid and more reliable diagnosis of these agents in the future. two agents not commonly thought of as causes of lri in children (rhinoviruses, coronaviruses) should now be added to an already long list. effective drugs exist for prophylaxis against influenza virus type a and therapy for influenza virus typ ... | 1994 | 8123226 |
| interactions between marek's disease virus encoded or induced factors and the rous sarcoma virus long terminal repeat promoter. | marek's disease virus transactivates promoters of avian leukemia and sarcoma viruses. in this study, a series of rsv-ltr promoter deletion mutants were used to map sites within the ltr important for mdv-mediated transactivation. mdv-responsive elements within rsv-ltr promoters were localized to a 28-bp segment (nucleotides -109 to -137) which contains a ggtgg pentanucleotide repeat element (pre). nuclear extract proteins from uninfected cells bound to the rsv pre in a sequence-specific manner. e ... | 1994 | 8116231 |
| antigenic diversity of respiratory syncytial viruses and its implication for immunoprophylaxis in ruminants. | bovine respiratory syncytial virus (brsv) is a very important pathogen of cattle and perhaps other ruminants. it is a major contributor to the incidence of respiratory tract disease in nursing beef and feedlot and dairy calves. the genome of respiratory syncytial viruses encodes 10 proteins translated from 10 unique mrnas. the major glycoprotein (g), fusion protein (f), 1a protein and the 22k protein are components of the viral envelope. the nucleocapsid contains the nucleocapsid protein (n), th ... | 1993 | 8116189 |
| nucleotide and predicted amino acid sequence analysis of the ovine respiratory syncytial virus non-structural 1c and 1b genes and the small hydrophobic protein gene. | respiratory syncytial virus (rsv) infects humans and cattle causing serious respiratory tract disease in both. the genome of the human and bovine rsv (hrsv and brsv) codes for two non-structural and eight structural proteins. rsv has also been isolated from naturally infected sheep, but the genome of the ovine rsv (orsv) has not been characterized and nor has the virus host range been identified. here we report on the cloning and sequencing of the two non-structural 1c and 1b genes and of the sm ... | 1994 | 8113762 |
| recombinant human respiratory syncytial virus (rsv) monoclonal antibody fab is effective therapeutically when introduced directly into the lungs of rsv-infected mice. | previously, recombinant human respiratory syncytial virus (rsv) monoclonal antibody fabs were generated by antigen selection from random combinatorial libraries displayed at the tip of filamentous phage. two such fabs, which exhibited high binding affinity for rsv f glycoprotein (a major protective antigen), were evaluated for therapeutic efficacy in infected mice just before or at the time of peak virus replication in the lungs. fab 19, which neutralized rsv infectivity with high efficiency in ... | 1994 | 8108420 |
| transactivation of the early sv40 promoter by avian infectious laryngotracheitis virus in avian hepatoma cells. | an avian hepatoma cell line has been reported to be suitable for the cultivation of avian laryngotracheitis virus (iltv) (scholz et al. (1993) j. virol. methods, 273-286; guo et al. (1993) am. j. vet. res., in press). to provide information for the establishment of avian expression systems and for the construction of avian recombinant viruses, five expression plasmids were constructed to test two avian viral and two mammalian viral promotors for their suitability and strength for gene expression ... | 1993 | 8106602 |
| clinical profile of pediatric patients hospitalized with respiratory syncytial virus infection. | to update the clinical profile of pediatric patients hospitalized with rsv infection, we retrospectively reviewed the records of 246 children (male:female ratio 1.44:1) admitted during one season to a tertiary-care hospital. the most common admitting diagnoses were bronchiolitis (37.4%), pneumonia (32.5%), and possible septicemia (13%). median age was 3 months; median length of stay, three days. twice as many minorities were admitted with rsv infection as all other admissions during the same yea ... | 1993 | 8104752 |
| overexpression of c-fos increases recombination frequency in human osteosarcoma cells. | we have shown previously that overexpression of c-ha-ras, v-mos or c-fos increases the spontaneous level of chromosomal aberrations and gene mutations in nih 3t3 cells, and that reduction of the fos protein level inhibits aberration induction by c-ha-ras and v-mos and also by irradiation with ultraviolet light (van den berg et al., mol. carcinogenesis, 4, 460-466). in order to examine whether fos is also involved in dna recombination, thymidine kinase (tk) deficient human osteosarcoma cells cont ... | 1993 | 8099316 |
| pathogenesis and treatment of bronchiolitis. | the pathogenesis, epidemiology, clinical features, sequelae, and treatment of bronchiolitis are reviewed. acute bronchiolitis is the most common severe lower-respiratory-tract infection of infancy. during epidemics, more than 80% of cases may be caused by respiratory syncytial virus (rsv). although signs and symptoms may become severe, most infections are self-limited and improvement occurs within several days. approximately 1-2% of infants less than one year of age require hospitalization. gene ... | 1993 | 8095871 |
| induction of local and systemic immunity against human respiratory syncytial virus using a chimeric fg glycoprotein and cholera toxin b subunit. | local iga and igg antibodies against respiratory syncytial virus (rsv) were induced in the respiratory tract of mice following intranasal vaccination with an rsv chimeric fg glycoprotein and cholera toxin b (ctb) as a mucosal adjuvant. local antibody production was not induced following parenteral immunization with fg administered in alum adjuvant. while both vaccination protocols induced serum antibodies against rsv and protected the lower respiratory tract from rsv infection, only intranasal f ... | 1994 | 8091852 |
| satisfactorily attenuated and protective mutants derived from a partially attenuated cold-passaged respiratory syncytial virus mutant by introduction of additional attenuating mutations during chemical mutagenesis. | a cold-passaged rsv mutant, designated cp-rsv, which acquired host range mutations during 52 passages at low temperature in bovine tissue culture, was completely attenuated for seropositive adults and children but retained the capacity to cause upper respiratory disease in seronegative infants. we sought to introduce additional attenuating mutations, such as temperature-sensitive (ts) and small-plaque (sp) mutations, into the cp-rsv mutant, which is a ts+ virus, in order to generate a mutant whi ... | 1994 | 8091846 |
| eosinophil cationic protein in nasopharyngeal secretions and serum of infants infected with respiratory syncytial virus. | eosinophil cationic protein (ecp) was assayed in nasopharyngeal secretion (nps) and serum from 42 infants, hospitalized with acute lower respiratory infection, in el salvador and the results analyzed in relation to etiology of the infection. ecp concentrations were high in nps, at an average 50 times higher than those found in serum. exceedingly high levels of ecp (> 1000 micrograms/l) were found more frequently in wheezing than in non-wheezing children (30% vs 7%) and, accordingly, were more co ... | 1994 | 8087188 |
| adenoviral-mediated gene transfer of human surfactant protein b to respiratory epithelial cells. | human surfactant protein b (sp-b) is a 79-amino acid, phospholipid-associated polypeptide expressed by respiratory epithelial cells of the lung. sp-b is essential for lung function, enhancing the spreading and stability of surfactant phospholipids that serve to reduce surface tension at the alveolar air-liquid interface. congenital absence of sp-b results in neonatal respiratory failure and death. in the present work, we constructed a replication-deficient adenoviral vector, av1sp-b1, in which t ... | 1994 | 8086169 |
| efficacy of adenovirus-vectored respiratory syncytial virus vaccines in a new ferret model. | in the absence of an adequate small animal model for testing the efficacy of adenovirus-vectored respiratory syncytial virus (rsv) vaccines, a ferret model was established for this purpose. recombinant adenovirus types 4, 5 and 7 expressing the rsv fusion glycoprotein (f), the attachment glycoprotein (g) or both f and g were constructed previously. these recombinants contain a deletion of a large portion of the e3 region of the respective adenovirus vector. in addition, an ad7(e3+)f recombinant ... | 1994 | 8085377 |
| the vbp and a1/ebp leucine zipper factors bind overlapping subsets of avian retroviral long terminal repeat ccaat/enhancer elements. | two long terminal repeat (ltr) enhancer-binding proteins which may regulate high rates of avian leukosis virus (alv) ltr-enhanced c-myc transcription during bursal lymphomagenesis have been identified (a. ruddell, m. linial, and m. groudine, mol. cell. biol. 9:5660-5668, 1989). the genes encoding the a1/ebp and a3/ebp binding factors were cloned by expression screening of a lambda gt11 cdna library from chicken bursal lymphoma cells. the a1/ebp cdna encodes a novel leucine zipper transcription f ... | 1994 | 8083963 |
| interaction of alveolar macrophages and respiratory syncytial virus. | respiratory syncytial virus (rsv) is a major cause of acute lower respiratory tract illness in infants. however, the mechanisms leading to resolution of rsv infections are poorly understood. since alveolar macrophages play an important role in defending the respiratory tract against infectious agents we investigated the interactions of rsv with these cells. murine alveolar macrophages were challenged in vitro with rsv at different multiplicities of infection. the percentage of macrophages expres ... | 1994 | 8083519 |
| src-specific immunity in inbred chickens bearing v-src dna- and rsv-induced tumors. | the growth pattern (progression/regression) of v-src dna- and rous sarcoma virus (rsv)-induced tumors was analogous on a panel of inbred chicken lines. the decisive role of the major histocompatibility complex [mhc(b)] alleles in resistance to the progression of these tumors was formally proved in segregating backcross populations. the immune mechanism of tumor regression was demonstrated by both in vivo and in vitro assays. a protective effect of v-src-specific immunity against rsv challenge wa ... | 1994 | 8082891 |
| tissue-specific expression of mouse tyrosinase gene in cultured chicken cells. | a mouse tyrosinase cdna has been combined with different promoters and inserted into several replication-competent avian leukosis proviruses and the viruses were transferred into cultured albino chick cells by viral infection. expression of the tyrosinase gene depended on one of four promoter sequences: the resident constitutive promoter (rous sarcoma virus long-terminal repeat; rsv-ltr), 471 bp from the mouse tyrosinase gene-associated promoter, 519 bp from the japanese quail tyrosinase gene as ... | 1994 | 8082718 |
| response of six major histocompatibility (b) complex recombinant haplotypes to rous sarcomas. | six b complex recombinants, br1 (f24-g23), br2 (f2-g23), br3 (f2-g23), br4 (f2-g23), br5 (f21-g19), and br6 (f21-g23), from the fourth backcross generation to highly inbred line ucd 003 (b17b17) were studied for their response to rous sarcomas. eight hatches were produced from heterozygous (brnb17) parents. chicks were wingweb inoculated with 50 pock-forming units of rous sarcoma virus (rsv) at 6 wk of age. a tumor profile index (tpi), based on degree of tumor regression, was evaluated by analys ... | 1994 | 8072926 |
| role of antibody and the use of respiratory syncytial virus immunoglobulin in the prevention of respiratory syncytial virus disease in preterm infants with and without bronchopulmonary dysplasia. | respiratory syncytial virus (rsv) is the only viral respiratory pathogen that produces an annual epidemic of respiratory illness. infants with cardiac disease or infants born prematurely with or without bronchopulmonary dysplasia are at increased risk of severe rsv disease. a recently developed rsv immunoglobulin (rsvig) was studied to determine safety and efficacy in prevention of severe rsv disease in such children who are high risk for severe rsv illness. results from this prospective, blinde ... | 1994 | 8072836 |
| passive protection against respiratory syncytial virus disease in infants: the role of maternal antibody. | respiratory syncytial virus (rsv) is responsible for serious respiratory disease in young infants. more than 75% of the 678 children hospitalized for rsv at baylor-affiliated hospitals in houston, tx, between october, 1992, and march, 1993, were 5 months of age or younger. the importance of maternal antibody in the immunity against rsv disease has been debated. more recent epidemiologic studies have demonstrated protection against rsv in babies born to mothers with high levels of neutralizing rs ... | 1994 | 8072835 |
| synthesis and antiviral activity evaluation of some aminoadamantane derivatives. | the synthesis of some spiro[cyclopropane-1,2'-adamantan]-2-amines and methanamines and some spiro[pyrrolidine-2,2'-adamantanes] is described. the title compounds were evaluated against a wide range of viruses (influenza a, influenza b, parainfluenza 3, rsv, hsv-1, tk- hsv-1, hsv-2, vaccinia, vesicular stomatitis, polio 1, coxsackie b4, sindbis, semliki forest, reo 1, hiv-1, and hiv-2), and some of them (compounds 6b, 6c, 9a, 16a, 16b, and 17) inhibited the cytopathicity of influenza a virus at a ... | 1994 | 8071937 |
| epithelial interleukin-11. regulation by cytokines, respiratory syncytial virus, and retinoic acid. | interleukin-11 (il-11) is a pleiotropic cytokine with effects that overlap with il-6. to determine if il-11 is produced by epithelial cells, we determined whether human alveolar a549 cells and airway 9hte cells produce il-11. we also determined whether retinoic acid (ra) altered this il-11 production. unstimulated cells produced low levels of il-11, while il-1, transforming growth factor (tgf-beta 1), and respiratory syncytial virus (rsv) stimulated il-11 protein production and mrna accumulation ... | 1994 | 8071352 |
| effects of the gag region on genome stability: avian retroviral vectors that contain sequences from the bryan strain of rous sarcoma virus. | we have previously described replication-competent schmidt ruppin-a rous sarcoma virus (rsv)-based retroviral vectors that can be used to deliver and express genes in avian cells. we have continued to modify the prototype vectors to develop a more versatile and efficient system. substitution of the polymerase (pol) region from the bryan high-titer rsv (bh-rsv) for the sr-a rsv pol region of these retroviral vectors causes these viruses to replicate more efficiently. we cloned the gag regions fro ... | 1994 | 8053145 |
| antigenic diversity of respiratory syncytial virus subgroup b strains circulating during a community outbreak of infection. | the epidemiological characteristics and relationship between respiratory syncytial virus (rsv) subgroup and virulence during an outbreak of rsv infection occurring in southeast texas in the winter season 1991/92 are described. fifty-two infants and children were diagnosed with rsv infection by rapid viral antigen detection and/or viral isolation. subgrouping of the isolates was carried out using 11-monoclonal anti-bodies. ten isolates were found to be subgroup b, and 8 isolates were subgroup a. ... | 1994 | 8046428 |
| effect of maternal antibody on iga antibody response in nasopharyngeal secretion in infants and children during primary respiratory syncytial virus infection. | the iga antibody response to respiratory syncytial virus (rsv) was determined in nasopharyngeal secretions (nps) of 22 infants and children infected with rsv group a strains, employing an elisa. the antibody activity observed during the convalescent phase against whole virus, fusion glycoprotein (f) and large glycoprotein (g) was examined in young infants (under 6 months) and compared with that of older individuals (6 to 16 months). both groups showed similar degrees of iga antibody activity to ... | 1994 | 8046418 |
| characterization of a molecular clone of a highly infectious avian leukosis virus. | a highly infectious avian leukosis virus (alv) has been molecularly cloned in a lambda phage and sequenced. in order to manipulate the genome of this alv and characterize the genetic determinants responsible for the high infectivity phenotype, a recombinant plasmid dna with the two ltr provirus was constructed. upon transfection of avian cells with this alv dna construct infectious viruses were produced as soon as 4 h after transfection and virus titer was 10(5) iu/ml after 24 h while that of th ... | 1993 | 8044699 |
| second-year surveillance of recipients of a respiratory syncytial virus (rsv) f protein subunit vaccine, pfp-1: evaluation of antibody persistence and possible disease enhancement. | in a previous study, children 18 to 36 months of age and seropositive for respiratory syncytial virus (rsv) were vaccinated with an rsv subunit vaccine (pfp-1) consisting of the viral fusion protein. vaccines developed substantial increases in anti-fusion and neutralizing antibody and exhibited protection against rsv infection through one rsv epidemic, in comparison to controls. this present study of the same cohort was undertaken to determine the persistence of antibody responses and immunity t ... | 1994 | 8036830 |
| enhanced pulmonary histopathology induced by respiratory syncytial virus (rsv) challenge of formalin-inactivated rsv-immunized balb/c mice is abrogated by depletion of interleukin-4 (il-4) and il-10. | in previous studies, children immunized with a formalin-inactivated respiratory syncytial virus vaccine (fi-rsv) developed severe pulmonary disease with greater frequency than did controls during subsequent natural rsv infection. in earlier efforts to develop an animal model for this phenomenon, extensive pulmonary histopathology developed in fi-rsv-immunized cotton rats and mice subsequently challenged with rsv. in mice, depletion of cd4+ t cells at the time of rsv challenge completely abrogate ... | 1994 | 8035532 |
| respiratory syncytial virus-specific cell-mediated immune responses after vaccination with a purified fusion protein subunit vaccine. | vaccination with a respiratory syncytial virus (rsv) fusion protein subunit vaccine (pfp-2) was done to determine if this vaccine induced evidence of cell-mediated immunity to rsv and if cell-mediated immunity prevented rsv reinfection. healthy children 12-18 months old received 50 micrograms of pfp-2 or a saline control. lymphocyte transformation (ltf) responses were determined before and 1 and 6 months after vaccination. pfp-2 induced positive ltf responses in 5 (83%) of 6 subjects whose preva ... | 1994 | 8035030 |
| humoral, mucosal, and cellular immune response to topical immunization with a subunit respiratory syncytial virus vaccine. | the efficacy of topical immunization with the respiratory syncytial virus (rsv) fusion (f) protein was tested in mice using cholera toxin b chain (ctb) as an adjuvant. the dose of ctb required for the adjuvant effect (as measured by local and systemic antibody stimulation) and protection from viral challenge was > or = 5 micrograms. with this dose, mice immunized intranasally with ctb plus f protein were highly protected from viral replication in the upper and lower respiratory tract. this prote ... | 1994 | 8035021 |
| an update on approaches to the development of respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3) vaccines. | rsv and piv3 are responsible for about 30% of severe viral respiratory tract disease leading to hospitalization of infants and children. for this reason, there is a need to develop vaccines effective against these viruses. since these viruses cause severe disease in early infancy, vaccines must be effective in the presence of maternal antibody. currently, several strategies for immunization against these viruses are being explored including peptide vaccines, subunit vaccines, vectored vaccines ( ... | 1994 | 8030364 |
| complex atrial tachycardias and respiratory syncytial virus infections in infants. | respiratory syncytial virus (rsv), a common cause of respiratory infections in children, has only rarely been associated with acquired heart disease. we reviewed hospital charts, rhythm strips, and electrocardiograms of 8 infants with abnormal supraventricular tachycardia (svt), > 250 beats/min, associated with acute rsv infections. cultures of nasopharyngeal specimens from six of eight infants grew rsv. two infants with negative viral culture results had symptoms typical of an rsv infection dur ... | 1994 | 8021780 |
| the lack of v-src involvement in tumorigenicity of marmoset cells transformed in vitro with rous sarcoma virus. | the transformation of nonhuman primate marmoset cells by rous sarcoma virus of schmidt-ruppin strain (rsv-sr) generates transformants which lack tumorigenicity in allo- and xenogeneic hosts. marmoset cells acquire this property when they are transformed by rsv rescued from non-tumorigenic allogeneic cells. one of the rescued rsv, when used to infect marmoset kidney cells in vitro, yielded transformants which became tumorigenic in adult allogeneic hosts. cytogenetic and molecular analyses of tran ... | 1994 | 8019956 |
| cloning of a 72 kda matrix metalloproteinase (gelatinase) from chicken embryo fibroblasts using gene family pcr: expression of the gelatinase increases upon malignant transformation. | chicken embryo fibroblasts secrete a 72 kda progelatinase that displays all of the characteristics of a matrix metalloproteinase. employing reverse-transcription pcr and degenerate oligonucleotide primers that are specific for two highly conserved sequences found in all matrix metalloproteinases, a dna fragment specific for the chicken gelatinase was generated. using this pcr product as a probe, cdna clones were isolated from a chicken embryo cdna library and the entire protein coding sequence w ... | 1994 | 8010954 |
| [respiratory syncytial virus infection. a frequent child disease in denmark with annual outbreaks]. | infection with respiratory syncytial virus (rsv) is an important cause of illness in infants often leading to hospital admission during the winter months. diagnosis of rsv by direct detection of virus antigen has been implemented in a number of departments of clinical microbiology, and on this background a descriptive study of the seasonal incidence, sex and age distribution of rsv in two danish counties was undertaken. a total of 5533 clinical episodes of respiratory infection were registered f ... | 1994 | 8009668 |
| respiratory syncytial virus (rsv) bronchiolitis: comparative study of rsv groups a and b infected children. | the grouping characteristics of 29 respiratory syncytial virus (rsv) present in nasopharyngeal cells collected from hospitalized children with bronchiolitis during the 1990 rsv season in porto alegre, rs, were analysed. twenty-two were grouped as belonging to group a and 7 to group b. cyanosis, oxygen therapy, cough, length of hospitalization and atelectasis were observed to be more frequently found within group b infected children. other clinical signs and symptoms were similarly found in both ... | 1994 | 8008913 |
| subgrouping of respiratory syncytial virus strains from australia and papua new guinea by biological and antigenic characteristics. | strains of respiratory syncytial virus from 3 major areas of australia and papua new guinea (png) were analyzed for variations in their antigenic and biological properties and in the molecular weights of their major structural proteins. seventy-eight strains from infants and young children with lri were collected from 1981-1984. the rsv season in the australian cities lasted from april through september, with major peaks in july of each year, while the rsv season in tropical png was year-round, ... | 1994 | 8002781 |
| [prevalence and clinical aspects of a and b subgroups of respiratory syncytial virus infection. observation of 8 consecutive epidemics between 1982 and 1990]. | infants suffering from respiratory syncytial virus (rsv) infection can have severe responses that require intensive care. this study compares the epidemiologic patterns and the severity of respiratory diseases produced by rsv strain subtypes a and b. | 1993 | 8002736 |
| [a retrospective survey on the respiratory course of severe bronchiolitis]. | respiratory syncytial virus (rsv) infection in infancy can induce bronchial reactivity and virus-specific ige production; these responses may favor the later development of asthma. this retrospective study examines the influences of early and severe forms of bronchiolitis on such an outcome. | 1993 | 8002720 |
| molecular cloning and sequence analysis of the phosphoprotein, nucleocapsid protein, matrix protein and 22k (m2) protein of the ovine respiratory syncytial virus. | respiratory syncytial viruses (rsvs) cause serious respiratory tract disease in infants and children worldwide and similar disease in calves. strains have been isolated from other ruminant animals such as sheep and goats, but these viruses have not been characterized at the molecular level. in this study, we report the cloning and sequencing of four structural genes coding for the phosphoprotein, nucleocapsid (n) protein, matrix (m) protein and 22k protein of an ovine rsv strain. comparisons of ... | 1994 | 7996153 |
| nucleotide sequence of rna 1, the largest genomic segment of rice stripe virus, the prototype of the tenuiviruses. | the complete nucleotide sequence of rna 1, the largest genomic segment of rice stripe virus (rsv), was determined using two sets of overlapping cdna clones. rna segment 1 comprises 8970 nucleotides and on the viral complementary sequence has a single long open reading frame coding for a protein of 2919 amino acids with an estimated m(r) of 336860. amino acid sequence comparisons of the putative protein indicated strong homology (30% amino acid identity over about 1500 residues) with the l protei ... | 1994 | 7996149 |
| alteration of pulmonary macrophage function by respiratory syncytial virus infection in vitro. | alveolar macrophages (al) are the first line of defense against inhaled pathogens and are exposed to virus during the course of a respiratory syncytial virus (rsv) infection. interference of virus with alveolar macrophage functions may contribute to the risk of acquiring secondary bacterial infections during or after respiratory tract infections with rsv or other viral agents. we studied whether murine al get infected with rsv and whether they support viral replication in vitro. in addition, the ... | 1995 | 7995946 |
| inhaled nitric oxide for a severe respiratory syncytial virus infection in an infant with bronchopulmonary dysplasia. | to report the first case of ards in children treated with nitric oxide (no) inhalation. | 1994 | 7995870 |
| mtt colorimetric assay system for the screening of anti-orthomyxo- and anti-paramyxoviral agents. | a rapid and sensitive method was developed for screening potential antiviral agents against orthomyxo- and paramyxoviruses, using the mtt method with cell culture suspensions. the cell lines used for the assay were as follows: mdck cells for the influenza a virus (fluv. a), hela cells for the respiratory syncytial virus (rsv), and vero cells for the measles virus (msv). test compounds were diluted and plated in 96-well round-bottomed microtiter plates. trypsinized cell suspensions and viruses we ... | 1994 | 7989442 |
| respiratory syncytial virus infection in hospitalized patients and healthy children in el salvador. | nasopharyngeal specimens from 42 children less than one-year old hospitalized with bronchiolitis or pneumonia in el salvador were analyzed for the presence of subgroup-specific respiratory syncytial virus (rsv) antigens by the indirect immunofluorescence technique. the antigen rsv-a was demonstrated in 28 children, rsv-b in three, and in one child subgroup, specificity could not be determined. the male:female ratio in the rsv-infected children was 1.9:1. the most severe disease, requiring intens ... | 1994 | 7985749 |
| attenuated temperature-sensitive respiratory syncytial virus mutants generated by cold adaptation. | two strains of respiratory syncytial virus (rsv), rsv 2b and rsv 3a (representing subgroup b and a virus respectively) were cold-adapted by passaging in vero cells for up to 42 weeks at successively lower temperatures down to 20 degrees c. successful cold adaptation of the virus population was dependent on the amount of time the cultures were maintained at the various low temperatures, as well as on the strain of virus used. temperature-sensitive (ts) mutants appeared in the cold passaged virus ... | 1994 | 7985411 |
| genetic heterogeneity of the attachment glycoprotein g among group a respiratory syncytial viruses. | fifteen independent group a respiratory syncytial virus (rsv) isolates were compared by sequencing a 300-nucleotide interval encoding a variable region of the attachment glycoprotein g. the viruses compared included the reference strains long (usa 1956), a2 (australia 1961), and 669 (sweden 1959), along with 13 clinical isolates obtained at different times and locations throughout the united states. representatives of all six antigenic subgroups, recognized by reactivity patterns with monoclonal ... | 1994 | 7985408 |
| viral respiratory infections. | viral infections constitute more than 60% of acute lower respiratory illnesses. respiratory syncytial virus (rsv) and parainfluenza viruses are the most frequent etiologic agents. after transmission by large droplet aerosol or direct contact, the viruses gain entry into host cells through specific viral surface proteins; subsequently, pathogenetic mechanisms cause tissue injury and result in clinical disease. in the intensive care unit the mainstay of treatment is primarily supportive. nonspecif ... | 1994 | 7984389 |
| update: respiratory syncytial virus activity--united states, 1994-95 season. | respiratory syncytial virus (rsv), a common cause of winter outbreaks of acute respiratory disease, causes an estimated 90,000 hospitalizations and 4500 deaths each year from lower respiratory tract disease in both infants and young children in the united states. outbreaks occur annually throughout the united states, and community activity usually peaks within 1 month of the national peak. rsv activity in the united states is monitored by the national respiratory and enteric virus surveillance s ... | 1994 | 7984143 |
| a further attenuated derivative of a cold-passaged temperature-sensitive mutant of human respiratory syncytial virus retains immunogenicity and protective efficacy against wild-type challenge in seronegative chimpanzees. | a cold-passage (cp), temperature-sensitive (ts) rsv mutant designated rsv cpts-248 (shut-off temperature 38 degrees c), which possesses host-range mutations acquired during 52 passages at low temperature in bovine tissue culture and a ts phenotype introduced by subsequent chemical mutagenesis, was found previously to be attenuated, immunogenic, and protective against wild-type challenge in seronegative chimpanzees. we sought to introduce additional attenuating mutations such as small-plaque (sp) ... | 1994 | 7975856 |
| requirement of casein kinase ii-mediated phosphorylation for the transcriptional activity of human respiratory syncytial viral phosphoprotein p: transdominant negative phenotype of phosphorylation-defective p mutants. | the transcription complex of the human respiratory syncytial virus was biochemically dissected and reconstituted in vitro with purified viral macromolecules. the minimal complex consisted of the viral n-rna template, viral phosphoprotein (p), and the large protein (l) along with host cellular factor(s), possibly actin. active transcription could also be reconstituted using bacterially synthesized recombinant p protein provided the p protein was phosphorylated by cellular casein kinase ii. elimin ... | 1994 | 7975205 |
| down-regulation of rous sarcoma virus long terminal repeat promoter activity by a hela cell basic protein. | we have previously isolated a hela cell cdna encoding a 21-kda polypeptide that is 48% similar to transcription factor iis. to explore the possibility that p21 plays a role in transcriptional regulation in vivo, we tested the effect of p21 expression on the synthesis of reporter chloramphenicol acetyltransferase (cat) in transfected cos-1 cells. cat formation under control of the rous sarcoma virus long terminal repeat (rsv ltr) promoter was decreased nearly 20-fold in cells coexpressing p21. in ... | 1994 | 7971997 |
| cytokine response to respiratory syncytial virus stimulation of human peripheral blood mononuclear cells. | a key impediment to developing respiratory syncytial virus (rsv) vaccines is a lack of understanding of enhanced disease that occurred in children who received a formalin-inactivated rsv (fi-rsv) vaccine. studies in mice have suggested that the fi-rsv vaccine induces a th2 and live rsv induces a th1 memory t cell response. in this study, the cytokine mrna response of peripheral blood mononuclear cells (pbmc) from adults and children with and without previous rsv infection was characterized using ... | 1994 | 7963714 |
| anti-il-4 treatment at immunization modulates cytokine expression, reduces illness, and increases cytotoxic t lymphocyte activity in mice challenged with respiratory syncytial virus. | upon respiratory syncytial virus (rsv) challenge, mice previously immunized intramuscularly with inactivated whole virus express a th2-like pattern of cytokine mrna, while mice immunized with live virus intranasally express a th1-like pattern. in this study, we evaluated the effects of anti-il-4 treatment on the induction of immune responses after immunization. mice treated with anti-il-4 at the time of immunization with inactivated rsv had reduced clinical illness after live virus challenge, as ... | 1994 | 7962541 |
| native timp-free 70 kda progelatinase (mmp-2) secreted at elevated levels by rsv transformed fibroblasts. | rous sarcoma virus-transformed cultures of chicken embryo fibroblasts (rsvcef) secrete elevated levels of a 70 kda progelatinase, an avian form of the 72 kda matrix metalloproteinase-2 (mmp-2). affinity-purified preparations of secreted 70 kda progelatinase are composed of two distinct populations of zymogen: a 70 kda progelatinase tightly complexed with an avian form of timp-2 and a native 70 kda progelatinase free of any detectable timp-2. these two forms of the progelatinase can be separated ... | 1994 | 7962125 |
| dexamethasone enhancement of gene expression after direct hepatic dna injection. | the critical physiological functions of the liver make hepatocytes important targets for therapeutic gene delivery. this study reports significant gene expression following direct injection of plasmid dna into the livers of rats and cats. transfection was characterized using luciferase and lac z expression from plasmids with the cytomegalovirus immediate early promoter/enhancer (cmv ie) or the rous sarcoma virus long terminal repeat (rsv ltr). dexamethasone treatment enhanced and prolonged trans ... | 1994 | 7961986 |
| effect of bovine respiratory syncytial virus infection on hypersensitivity to inhaled micropolyspora faeni. | respiratory syncytial virus causes mild-to-severe respiratory disease in human infants and young children; a closely related bovine respiratory syncytial virus causes a similar disease pattern in calves. increased disease severity in atopic children suggests that allergic reactivity may enhance the severity of rsv-induced disease. to examine the association between bovine respiratory syncytial virus (brsv) infection and allergic reactivity two groups of calves were exposed to aerosolized micropo ... | 1994 | 7950409 |
| purification of a recombinant human respiratory syncytial virus chimeric glycoprotein using reversed-phase chromatography and protein refolding in guanidine hydrochloride. | fg glycoprotein is a recombinant chimeric protein consisting of the extracellular portions of human respiratory syncytial virus (rsv) f and g glycoproteins. in theory, highly purified fg glycoprotein may be effective as a rsv vaccine. recombinant fg glycoprotein was expressed using the baculovirus/insect cell system. fg glycoprotein was isolated from cell culture supernatants using s sepharose ion-exchange chromatography, cu(2+)-immobilized metal affinity chromatography, preparative reversed-pha ... | 1994 | 7950387 |
| gene transfer into skeletal muscles by isogenic myoblasts. | the best way to overcome immunorejection in heterologous myoblast transfer (hmt) is by the use of immunodeficient and/or highly immunosuppressed mice as hosts. the same may be attained by autologous myoblast transfer (amt). in this paper, we describe myoblast transfer in mdx and normal mice where the donor myogenic cells originated from highly inbred litter mates that are considered to be isogenic and thus the procedure is analogous to amt. the myoblasts were marked in vitro with rous sarcoma vi ... | 1994 | 7948144 |
| the role of respiratory syncytial virus in acute bronchiolitis in small children in northern japan. | respiratory syncytial virus (rsv) plays an important role in acute bronchiolitis, which is life threatening in some infants. we investigated the epidemiology of rsv acute bronchiolitis in children less than 3 years old in northern japan. from april 1991 to march 1993, 162 infants with acute bronchiolitis were hospitalized in our pediatric wards. the diagnosis of rsv acute bronchiolitis was based on the typical clinical manifestations and the presence of rsv antigen in their nasopharyngeal specim ... | 1994 | 7941998 |
| genome organization of a biologically active molecular clone of the lymphoproliferative disease virus of turkeys. | the lymphoproliferative disease retrovirus (lpdv) induces an acute, horizontally transmitted disease of turkeys that is often fatal. although lpdv cannot be grown in cultured cells, it was possible to isolate molecular clones of biologically active integrated proviral genomes from spleens of infected turkeys. based upon molecular hybridization and nucleotide sequence comparisons of its pol gene, lpdv was shown to represent a distinct group of avian retroviruses most closely related to avian sarc ... | 1994 | 7941337 |
| national surveillance for respiratory syncytial virus, united states, 1985-1990. | respiratory syncytial virus (rsv) causes pneumonia and bronchiolitis in infants and young children and serious disease in the elderly and persons with compromised immune systems. to determine the temporal and geographic patterns of rsv outbreaks in the united states, monthly reports from 74 laboratories were analyzed for july 1985 through june 1990. rsv outbreaks were identified in 197 (93%) of the 211 laboratory years analyzed, with widespread activity beginning each fall, peaking in winter, an ... | 1994 | 7930745 |
| effect of respiratory syncytial virus-antibody complexes on cytokine (il-8, il-6, tnf-alpha) release and respiratory burst in human granulocytes. | the release of interleukin-8 (il-8), il-6, and tumour necrosis factor-alpha (tnf-alpha) from human polymorphonuclear granulocytes (pmn) after exposure to infectious respiratory syncytial virus (rsv) particles was investigated. our data showed that pmn secreted il-8 and il-6 in a time- and rsv-dose-dependent manner. during the rsv exposure, tnf-alpha was not detected in the cell supernatant of pmn. similar amounts of il-8 were secreted after either incubation with infectious or uv-inactivated rsv ... | 1994 | 7927487 |
| [detecting respiratory syncytial virus in respiratory epithelial cells in adult patients by electron microscopy and immune electron microscopy]. | in order to evaluate the value of electron microscopy (em) in diagnosing respiratory syncytial virus (rsv) infection in adults, the exfoliated cells from nasopharynx of 97 rsv positive patients were examined by em and the gold labelling technique for immune electron microscopy. the viral particles with rsv features were found at the surface of the exfoliated cells only in a few samples by em, and the specific gold labelling were observed in the immune electron microscopy (iem). the results showe ... | 1993 | 7923432 |
| multinucleated cells formed in vitro from paget's bone marrow express viral antigens. | paget's disease of bone is characterized by large numbers of osteoclasts that have viral-like nuclear and/or cytoplasmic inclusions. pagetic osteoclasts express respiratory syncytial viral (rsv) and measles viral (mv) nucleocapsid antigens. the data suggest a possible viral etiology for paget's disease. however, studies to characterize further the putative viral inclusions in paget's osteoclasts have been severely hampered by the extreme difficulty in isolating large numbers of osteoclasts from ... | 1994 | 7917585 |
| liposome-mediated dna transfer into chicken primordial germ cells in vivo. | in embryogenesis, avian primordial germ cells (pgcs) circulate temporarily in the blood vessels at stages 10-15 (hamburger and hamilton, 1951), before reaching the gonads. in an attempt to transfer cloned genes into pgcs, liposome consisting of reporter plasmid dna and n-[1-(2,3-dioleoyloxy)propyl]-n,n,n-trimethylammoniummethylsulf ate was injected into the marginal veins of embryos at stages 11-15. as reporter plasmids, prsvz and pacz harboring the escherichia coli lacz gene driven, respectivel ... | 1994 | 7917278 |
| nosocomial respiratory syncytial virus infections in a paediatric department. | the seasonal problem of respiratory infections in children caused by respiratory syncytial virus (rsv) is worldwide. a number of these infections are known to occur by nosocomial acquisition. in order to reduce the risk, measures, such as cohort nursing and handwashing, have been used in the paediatric department of odense university hospital for three years. in a retrospective evaluation of this routine practice the incidence of nosocomial rsv infections was recorded. the overall rate of nosoco ... | 1994 | 7911483 |
| [respiratory syncytial virus (rsv) infection: a diagnostic and therapeutic challenge. proceedings of an international symposium. wurzburg, 11 september 1993]. | 1994 | 7910685 | |
| a novel cis element mediating ligand-independent activation by c-erba: implications for hormonal regulation. | a novel type of hormone-responsive element (hre) is described. unlike classical hres, this element, rsv-t3re (found in rous sarcoma virus-long terminal repeat), mediates strong activation by the c-erba alpha thyroid hormone (t3) receptor in the absence of t3, and addition of t3 reverses this response. whereas both c-erba alpha and v-erba are potent ligand-independent activators through the rsv-t3re, c-erba beta is not. the rsv-t3re is recognized and activated by either c-erba alpha homodimers or ... | 1993 | 7903219 |
| respiratory syncytial virus or influenza? | we compared data from clinical surveillance of acute respiratory infections in elderly people reported by a network of sentinel general practitioners, virus isolate data for respiratory syncytial virus (rsv) and influenza a and b viruses, and registered deaths, during the past four winters. cases of acute respiratory disease (ard) and deaths (3-week moving averages) showed strong similarity in timing. periods of high incidence of ard and numbers of deaths were followed within 2-3 weeks by report ... | 1993 | 7902899 |
| effect of ionizing radiation on the expression of chloramphenicol acetyltransferase gene under the control of commonly used constitutive or inducible promoters. | we examined the effect of ionizing radiation on the expression of the chloramphenicol acetyltransferase (cat) gene driven by commonly used viral or semi-synthetic promoters in transient transfection assays using cos-7 cells. specifically, we studied the constitutive early promoters of cytomegalovirus (cmv), rous sarcoma virus (rsv), and the simian virus 40 (sv40), and the inducible promoters from mouse mammary tumour virus (mmtv, inducible by dexamethasone) and the semi-synthetic svlaco promoter ... | 1995 | 7897274 |
| respiratory syncytial virus and allergic conjunctivitis. | the pathogenesis of allergic conjunctivitis is largely conjectural. we investigated the possible involvement of respiratory syncytial virus (rsv), a ubiquitous respiratory pathogen, in the development of allergic conjunctivitis through immune mechanisms. a new technique of brush cytology was used to obtain conjunctival cells from 30 patients with allergic conjunctivitis and 20 control subjects. samples were assayed for the presence of rsv sequences with the reverse-transcription polymerase chain ... | 1995 | 7897147 |
| epidermal growth factor receptors in rous sarcoma virus-transformed cells of different hosts. | it was found that epidermal growth factor (egf) binding on cells transformed by rous sarcoma virus (rsv) differed in mice and hamsters. while egf binding was considerably reduced in mouse tumours, the binding activity of hamster cells did not change after rsv transformation. | 1994 | 7895858 |
| evidence for the role of glycoprotein g of respiratory syncytial virus in binding of neisseria meningitidis to hep-2 cells. | viral glycoproteins g and f are expressed on the surface of cells infected with respiratory syncytial virus (rsv). we investigated the role of these proteins in the previously reported enhanced binding of neisseria meningitidis to rsv-infected hep-2 cells. virus particles attached to bacteria were detected by immunofluorescence with flow cytometry. binding of fitc-labelled bacteria to rsv-infected cells was significantly inhibited by monoclonal antibody against glycoprotein g. unlabelled bacteri ... | 1994 | 7874075 |
| adenovirus-mediated gene therapy for human head and neck squamous cell cancer in a nude mouse model. | adenovirus-mediated transfer of the herpes simplex virus thymidine kinase gene followed by ganciclovir administration was used to treat human head and neck cancer in nude mice. tumors were generated by transcutaneous needle injection of 6 x 10(6) human squamous carcinoma cells into the floor of the mouth. after 14 days, 10(10) particles of a replication-defective recombinant adenovirus containing the herpes simplex virus thymidine kinase gene (adv/rsv-tk) were injected directly into the tumors. ... | 1995 | 7866992 |
| respiratory syncytial virus and allergic conjunctivitis. | the pathogenesis of allergic conjunctivitis is largely conjectural. we investigated the possible involvement of respiratory syncytial virus (rsv), a ubiquitous respiratory pathogen, in the development of allergic conjunctivitis through immune mechanisms. brush cytology technique was used to obtain conjunctival cells from 30 patients with allergic conjunctivitis and 20 controls. samples were assayed for the presence of rsv sequences with the reverse transcription polymerase chain reaction (rt-pcr ... | 1994 | 7861690 |
| inhibition of melanoma growth by adenoviral-mediated hsv thymidine kinase gene transfer in vivo. | to assess the potential of an in vivo, adenovirus-mediated gene therapy approach for the treatment of malignant melanoma, the efficacy of adenovirus-mediated herpes simplex virus thymidine kinase gene (hsv-ek) transfer and administration of ganciclovir (gcv) was investigated using a nude mouse model. initially, b16 murine melanoma cells were efficiently transduced in vitro by a recombinant replication-defective adenovirus containing the hsv-tk gene (adv/rsvtk), and rendered sensitive to cell kil ... | 1995 | 7860993 |
| [rapid detection for antigens of respiratory tract viruses by using series of monoclonal antibody]. | a series of monoclonal antibodies (mcab) against six main respiratory tract viruses(rtv)--respiratory syncytial virus (rsv), influenza a virus (flua), influenza b virus(flub), parainfluenza virus type 1.2.3 (piv1.2.3) were developed, and were used in apaap bridge-enzyme assay to rapidly detect the antigens of rtv. total 260 samples of nasopharyngeal exfoliated cells were collected from young men in beijing army suffered from respiratory tract virus infections. the results showed that 18 cases we ... | 1994 | 7859266 |
| [ambisense rna genome of rice stripe virus]. | 1994 | 7856115 | |
| a suboptimal src 3' splice site is necessary for efficient replication of rous sarcoma virus. | regulation of splicing of rous sarcoma virus (rsv) rna primary transcripts is necessary, as with other retroviruses, to allow for the accumulation of unspliced rna and approximately equivalent amounts of spliced env and src mrnas. previous studies have indicated that the env 3' splice site is suboptimal because it has a nonconsensus branchpoint sequence and that this suboptimal splice site is required for virus replication (r. a. katz and a. m. skalka, 1990, mol. cell biol. 10:696-704). we show ... | 1995 | 7856084 |
| validation of respiratory syncytial virus enzyme immunoassay and shell vial assay results. | the pathfinder respiratory syncytial virus (rsv) enzyme immunoassay (eia) (kallestad), the shell vial (sv) technique, and conventional cell culture (cc) were compared for detection of rsv in nasopharyngeal aspirates. we found sensitivities, specificities, and positive and negative predictive values of 58.4, 100, 100, and 68.2%; 80.7, 97.2, 97.0, and 81.9%; and 77.6, 97.2, 96.9, and 79.5% for the cc, eia, and sv methods, respectively. the sv and eia techniques were both more sensitive than cc (p ... | 1994 | 7852589 |
| the etiology of pneumonia in malnourished and well-nourished gambian children. | during a 2-year period 159 malnourished children ages 3 months to 5 years with radiologic evidence of pneumonia were investigated to determine the cause of their pneumonia. in addition 119 malnourished children without pneumonia, 119 well-nourished children with pneumonia and 52 well-nourished children without pneumonia were studied as controls. percutaneous lung aspiration was performed on 35 malnourished and 59 well-nourished children with pneumonia. bacteria were isolated from the blood, lung ... | 1994 | 7845751 |
| pediatric investigators collaborative network on infections in canada (picnic) prospective study of risk factors and outcomes in patients hospitalized with respiratory syncytial viral lower respiratory tract infection. | to provide information on disease attributable to respiratory syncytial viral lower respiratory tract infection (rsv lri) and to quantify the morbidity associated with various risk factors. | 1995 | 7844667 |
| systemic immunoprophylaxis of nasal respiratory syncytial virus infection in cotton rats. | the cotton rat model was used to test whether systemically administered immunoglobulin could protect nasal tissues against low challenge doses of respiratory syncytial virus (rsv). animals were pretreated by intraperitoneal injection of human immunoglobulin with moderate (1:2226) or high (1:15,000) neutralizing antibody titers to rsv (day 0), challenged intranasally with rsv long at doses ranging from 10(1) to 10(5) pfu (day 1), and sacrificed for virus titration (day 5). pretreatment with moder ... | 1995 | 7844385 |
| [respiratory syncytial virus infections in children]. | respiratory syncytial virus (rsv) is the most frequently isolated agent in acute lower respiratory tract infections in children. it is responsible for severe disease in young infants and patients with immune deficiencies or congenital heart disease, and appears to be involved in sudden death in infancy. there is also some evidence for its involvement in the development of asthma following bronchiolitis. despite encouraging new therapies (ribavirin, immune globulin, recombinant interferon alfa), ... | 1994 | 7842109 |
| viral, mycoplasmal and chlamydial lower respiratory tract infections in hong kong: cost and diagnostic value of serology. | a hospital virology laboratory in hong kong tested 10,852 sera by complement fixation (cf) for antibodies against agents of respiratory disease between 1 july 1986 and 31 december 1991. ten commercially supplied antigens were used: influenza virus types a and b, parainfluenza virus types 1, 2 and 3, adenovirus, respiratory syncytial virus (rsv), mycoplasma pneumoniae, chlamydia species and coxiella burnetii. single sera comprised 69% of the total, including sera from 7488 patients investigated f ... | 1994 | 7841840 |
| eosinophil cationic protein in nasal secretion and in serum and myeloperoxidase in serum in respiratory syncytial virus bronchiolitis: relation to asthma and atopy. | eosinophil cationic protein (ecp) in nasal secretions was determined in 34 infants with respiratory syncytial virus (rsv) bronchiolitis during the acute infection stage and one and six months later. ecp in serum was determined in 19 of these children at the same time. myeloperoxidase (mpo) was determined in the same 19 children at the acute infection stage and after one month. all children were followed prospectively for two years after the infection with regard to the development of bronchial o ... | 1994 | 7841728 |
| detection of respiratory syncytial virus in children in the 1993-94 winter season in izmir, turkey, by two diagnostic methods. | respiratory syncytial virus (rsv) infection was searched for in 65 children between 2 months and 2 years of age hospitalized with the presumptive diagnosis of acute bronchiolitis, and in 35 children aged 6 months to 8 years with upper respiratory tract symptoms, by direct fluorescent antibody (dfa) test and cell culture. rsv infection was detected in 29.2% of the first and in 11.4% of the second group. the overall positivity rate was 23%. the sensitivity and the specificity of the commercially a ... | 1994 | 7833009 |
| the leukocyte response of japanese quail to rous sarcoma virus-induced tumors. | in male japanese quail, different circulating leukocyte responses were observed for progressors (birds developing a massive tumor that persisted throughout the experiment) and regressors (birds developing a tumor that gradually disappeared) after initial challenge with rous sarcoma virus (rsv). blood was sampled before and at weekly intervals postinoculation. blood smears were prepared and stained with diff quik, and a light microscope (1000 x) was used in a direct count of 50 fields. leukocytes ... | 1994 | 7832716 |
| activities and substrate specificity of the evolutionarily conserved central domain of retroviral integrase. | the retroviral integrase (in) is a virus-encoded enzyme that is essential for insertion of viral dna into the host chromosome. in order to map and define the properties of a minimal functional domain for this unique viral enzyme, a series of n- and c-terminal deletions of both rous sarcoma virus (rsv) and human immunodeficiency virus (hiv) ins were constructed. the rsv in deletion mutants were first tested for their ability to remove two nucleotides from the end of a substrate representing the t ... | 1995 | 7831800 |
| treatment of respiratory syncytial virus infections in children. | treatment of the infections caused by the respiratory syncytial virus (rsv) has varied largely in different centres. recently, however, management practices have become more clear based on a number of studies. an infant with rsv bronchiolitis should be hospitalized in case of insufficient oxygenation, as measured by pulse oximetry, and additional oxygen should be supplied. mist treatment and physiotherapy are not beneficial. bronchodilators seem to be the drug of choice in most infants with bron ... | 1994 | 7826594 |
| 3-cyclopentyl-1-adamantanamines and adamantanemethanamines. antiviral activity evaluation and convulsions studies. | the synthesis of 3-cyclopentyl-1-adamantanamines and adamantanemethanamines and some of their thioureas is described. the antiviral activity examination of these compounds indicated that some of them inhibited respiratory syncytial virus (rsv) infection at concentrations that were slightly (up to 5-fold) lower than the cytotoxic concentration. behavioral and convulsions studies of the above mentioned amines, in mice, did not show any dopaminomimetic activity and argue in favor of the existence o ... | 1994 | 7826470 |
| comparison of various transport media for viability maintenance of herpes simplex virus, respiratory syncytial virus, and adenovirus. | seven viral transport media (vtm) were compared for effectiveness in preserving the infectivity of herpes simplex virus (hsv), respiratory syncytial virus (rsv), and adenovirus. the media tested were richards viral transport, sucrose-phosphate-glutamate (spg), virocult, hh medium, tryptose phosphate broth, cell culture medium, and bartel's viral transport. two laboratory strains of hsv (mcintyre and 333) and two clinical isolates (a0301 and a0386), comprising two hsv-1 types and two hsv-2 types, ... | 1994 | 7820992 |
| [development of pcr tests for the detection of bovine herpesvirus-1, bovine respiratory syncytial viruses and pestiviruses]. | the development of pcr assays for detection of bhv-1, brsv, bvdv and another pestiviruses is summarized. a polymerase chain reaction assay based on primers selected from the viral gi glycoprotein gene detected 3 fg pure bhv-1 dna, 0.1-1.0 tcid50 or a single infected cell. no amplification was observed with dna from bhv-2, bhv-3, bhv-4, ohv-1 or ohv-2. however, a fragment of the correct size (468 bp) was amplified using dna from herpesviruses isolated from reindeer, red deer and goat. the pcr ass ... | 1994 | 7817501 |
| neutralization of respiratory syncytial virus after cell attachment. | little is known about the mechanisms of antibody-mediated neutralization of respiratory syncytial virus (rsv) which causes recurrent infections in human despite the virtually universal presence of neutralizing serum antibodies. human serum neutralization titers showed strong correlation with post-cell-attachment neutralizing titers for both rsv-convalescent sera and control sera but showed less strong correlation with cell-attachment blocking titers. neutralization was effective for the first 60 ... | 1995 | 7815504 |
| cytotoxic t cells specific for a single peptide on the m2 protein of respiratory syncytial virus are the sole mediators of resistance induced by immunization with m2 encoded by a recombinant vaccinia virus. | we have studied the immunobiology of respiratory syncytial virus (rsv), a major cause of respiratory tract morbidity in children. as part of these studies, it was previously found that immunization of balb/c (h-2d) mice with a recombinant vaccinia virus (rvv) which encoded the m2 protein of rsv provided complete protection against infection with rsv. this protection was transient and associated with m2-specific cd8+ t-cell (tcd8+) responses. in this study, we used two approaches to demonstrate t ... | 1995 | 7815502 |
| development of nested pcr assays for detection of bovine respiratory syncytial virus in clinical samples. | two nested pcr assays were developed for the detection of bovine respiratory syncytial virus (brsv). primers were selected from the gene encoding the f fusion protein (pcr-f) and the gene encoding the g attachment protein (pcr-g). biotinylated oligonucleotide probes, termed f and g, were selected for the hybridization of the respective pcr products. the sensitivities of the pcr-f and pcr-g assays were similar, both detecting 0.1 tissue culture infective dose of the virus. the pcr-f assay amplifi ... | 1994 | 7814551 |
| safety and immunogenicity of a subunit respiratory syncytial virus vaccine in children 24 to 48 months old. | a subunit vaccine for respiratory syncytial virus (rsv) consisting of purified fusion glycoprotein (designated pfp-1) was tested in children 24 to 48 months old. two doses of 20 micrograms (n = 13) and 50 micrograms (n = 10) were compared with a saline (n = 24) placebo control group. local and systemic reactions, reported within 96 hours postvaccination, were mild, transient, and did not differ significantly from the control cohort. long term follow-up through at least one, and in some cases two ... | 1994 | 7808848 |