Publications

effects of endotoxin and extracts of pregnant mouse uterus on the recovery of hemopoiesis after 5-fluorouracil.	a single injection of endotoxin, 4 hours after administration of 150 mg/kg of 5-fluorouracil (5-fu), stimulated the recovery of bone marrow hemopoietic cells that form colonies in spleen (cfu-s) or in culture (gm-cfc), of erythropoiesis, and of platelet production. corynebacterium parvum injections had similar effects. endotoxin-free extracts of tissues (from pregnant mouse uterus, placenta, and embryo) which have a high content of the factor(s) capable of stimulating growth of gm-cfc in vitro a ...	1979	316724
[the bacteriological and biological significance of propionibacterium acnes--the indigenous bacteria of human skin. iv. age-related change in antibody to p. acnes in normal human sera (author's transl)].		1979	316030
association of host immunoglobulins with solid tumours in vivo.	using a direct radioimmune antiglobulin technique and a competitive double-antibody radioimmune assay, we have demonstrated the presence of appreciable amounts of host immunoglobulins on the surface and in extracts of cell suspensions from freshly excised solid tumours. iga appeared to have the greatest concentrations from freshly excised solid tumours. iga appeared to have the greatest concentration, followed in turn by igm congruent to igg2a greater than igg1 congruent to igg2b greater than ig ...	1979	315785
value of immune monitoring in gynecologic cancer patients receiving immunotherapy.	immunotherapy with nonspecific immunopotentiators such as corynebacterium parvum is under study in several areas of gynecologic oncology. the efficacy of this new modality awaits the results of carefully done clinical trials designed to measure an improved effect on survival when this and similar agents are added to conventional therapy. this report deals with an attempt to demonstrate enhancement of the cell-mediated immune response in a small number of patients with advanced ovarian (18 patien ...	1979	315714
[preliminary considerations on the intravenous use of corynebacterium parvum in the treatment of solid neoplasms].		1979	315598
stimulation of phosphatidylinositol turnover in the macrophage plasma membrane: a possible mechanism for signal transmission.		1979	315365
effects of repeated corynebacterium parvum and bcg therapy on immune parameters: a weekly sequential study of melanoma patients. i. changes in non-specific (nk, k and t cell) lymphocytotoxicity, peripheral blood counts and delayed hypersensitivity reactions.	increased lymphocyte cytotoxicity, particularly of 'killer' k cell type was recorded with repeated immunizations of either c. parvum or bcg. a 3 week interval between immunizations was capable of maintaining the increase in cytotoxicity. no marked alterations of 'recall' skin hypersensitivity reactions nor of peripheral blood counts were noted. expression of cytotoxicity results as percentage 51cr release, lytic units/ml and cytotoxic capacity (after logit transformation of the cytotoxicity-lymp ...	1979	314367
antigenic bacterial polysaccharide in rheumatoid synovial effusions.	phenol-water extracted rheumatoid synovial fluids and synovial fluid leukocytes contain an antigen immunologically identical to the proprionibacterium group bacteria. the antigen was identified by counter-immunoelectrophoresis in 70% of rheumatoid synovial fluid leukocyte pellets and in 60% of rheumatoid synovial fluids. it was also present in 6% of nonrheumatoid fluids and in 22% of nonrheumatoid inflammatory fluid leukocytes. antigen was not detectable in synovial samples before extraction. sy ...	1979	314293
a trial of 5-fluorouracil and corynebacterium parvum in advanced colorectal carcinoma.	this study has confirmed that patients who have advanced colorectal carcinoma have impaired responsiveness to delayed-hypersensitivity skin testing, and also have elevated levels of serum igm. serial observations of delayed-hypersensitivity skin tests, total lymphocyte counts, t-lymphocyte counts, b-lymphocyte counts, and serum immunoglobulin levels failed to reveal any consistent pattern of responses in patients treated with either chemotherapy alone or chemoimmunotherapy. in 33 patients chosen ...	1979	313873
immunologic evaluation of patients with advanced head and neck cancer receiving weekly chemoimmunotherapy.	patients with advanced head and neck cancer have significant reduction in their circulating lymphocyte mass which is reflected in decreased numbers of t cells, fc receptor cells, and in derangements of t lymphocyte functions, i.e., decreased responsiveness to several dilutions of phytohemagglutinin and lack of development of delayed hypersensitivity to 2,4-dinitrochlorobenzene (70% of patients were dncb (-)). a population of phagocytic cells capable of decreasing lymphocyte responsiveness to mit ...	1979	313240
anaerobic and aerobic bacteriology of acute conjunctivitis.	aerobic and anaerobic cultures and clinical data were obtained from 131 patients presenting with acute conjunctivitis. similar cultures were obtained from 60 noninflamed individuals. anaerobes were isolated from 66 patients, 51 times in mixed culture with aerobes and 15 times (11.5%) as the only isolates. aerobes only were recovered in 54 patients. the organisms found to be statistically significantly more commonly recovered from eyes with conjunctivitis were staphylococcus aureus, hemophilus in ...	1979	313179
effects of corynebacterium parvum and bcg therapy on immune parameters in patients with disseminated melanoma. a sequential study over 28 days. ii. changes in non-specific (nk, k and t cell) lymphocytoxicity and delayed hypersensitivity skin reactions.	c. parvum and bcg produced significant changes in nk, k and t cell lymphocytotoxicity using a chang liver target cell. a consistent temporal pattern over 28 days of early depression, recovery, overshoot and then decline was described. this was particularly marked for c. parvum and 'k' cell activity. skin test reactivity to recall antigens at 28 days was not appreciably different from the pre-immunization reactivity. the importance of using lymphocyte concentration-cytotoxicity titration curves a ...	1979	312169
separation of functionally distinct subpopulations of corynebacterium parvum-activated macrophages with predominantly stimulatory or suppressive effect on the cell-mediated cytotoxic t cell response.		1979	312146
immune function determination in patients with bladder cancer.	patients with bladder cancer were evaluated clinically for immune responsiveness and stage of disease. a battery of tests is described that attempts to correlate stage of disease with immune responsiveness.	1978	311893
inhibition of memory cell-mediated cytotoxic response by systemic administration of corynebacterium parvum.		1979	311692
effect of corynebacterium parvum on human t-lymphocyte interferon production and t-lymphocyte proliferation in vitro.		1978	309789
a role for t lymphocytes in tumour inhibition and enhancement caused by systemic administration of corynebacterium parvum.		1978	308542
induction of interferon production in mouse spleen cell cultures by corynebacterium parvum.		1978	307432
[immunotherapy of malignant diseases. a way towards the goal?].		1978	305530
possible mechanisms in the anti-tumour activity of c. parvum.	normal murine peritoneal macrophages exhibited anti-tumour cytotoxicity following simultaneous incubation with c. parvum and lymphoid cells from c. parvum-treated mice. both t- and b-lymphocytes appear to be involved in this macrophage activation which is mediated by a soluble factor(s) released by immune lymphoid cells in response to the specific immunising strain of c. parvum. cells capable of macrophage activation do not appear until 6 days following systemic c. parvum administration despite ...	1977	305375
a role for t lymphocytes in the antitumour action of systemic c. parvum.	the frequency of tumours arising from s.c. injection of a syngeneic chemically-induced fibrosarcoma (fsa) was not influenced by systemic administration of c. parvum (day + 3) except when doses less than the td50 were injected. then the number of takes was increased. the tumour normally grows progressively however regression was frequent in intact mice treated with c. parvum. tumour regression did not occur in t cell-depleted mice treated in the same way. splenic t cell-enriched populations of ce ...	1977	305374
effects of corynebacterium parvum on granulocyte/macrophage production and toxicity of chemotherapy.		1977	305373
suppression of rat t cell proliferation by corynebacterium parvum: t cell requirement for induction.		1978	304893
colony stimulating and inhibiting activities in mouse serum after corynebacterium parvum-endotoxin treatment.		1978	304892
[the so-called adjuvant corynebacterium parvum].		1978	304802
altered toxicity of 5-fluorouracil following treatment with corynebacterium parvum.	recent studies have demonstrated that systemic corynebacterium parvum increases serum granulocyte-macrophage colony-stimulating factor and stimulates the proliferation of granulocyte-macrophage progenitor cells. it was hypothesized that more rapid cycling of granulocyte-macrophage progenitor cells would render the cells more sensitive to a cell cycle-specific chemotherapeutic agent. the colony-forming ability of bone marrow granulocyte-macrophage progenitor cells was assayed in vitro with soft a ...	1978	304762
immunotherapy of cancer.		1977	301988
effect of corynebacterium parvum on colony-stimulating factor and granulocyte-macrophage colony formation.	because corynebacterium parvum has tumor-inhibitory properties and stimulates granulocyte-macrophage production, it may have clinical value in combination with chemotherapy. the leukopoietic effect of killed suspensions of c. parvum was studied in mice using the technique of in vitro clonal culture of hematopoietic cells. after c. parvum injection, there was a prompt, sustained elevation of serum colony-stimulating factor followed by an increase in granulocyte-macrophage precursor cells in the s ...	1977	300651
[is immunotherapy useful in bronchial carcinoma?].		1977	300502
in vitro antitumor effect of lymphoid cells from corynebacterium parvum-treated mice: effect of route of c. parvum administration.	corynebacterium parvum administration in cba mice resulted in the stimulation in the peritoneal exudate and other lymphoid organs of cells which nonspecifically inhibited the tumor growth in vitro. the distribution of the antitumor activity in different organs was dependent on the route of c. parvum injection. both ip and iv treatments stimulated antitumor activity in the peritoneal exudate, whereas the sc route was ineffective. only iv treatment stimulated the antitumor activity in the blood, ...	1977	300113
effect of corynebacterium parvum on tumor growth in normal and athymic (nude) mice.	the effect of systemic or local injection of corynebacterium parvum at the tumor site on the growth of various murine tumors was studied in intact and congenitally athymic balb/c mice. systemic injection of c. parvum usually had a marked antitumor effect in both types of mouse. two lymphomas, which regressed spontaneously in untreated intact mice but not in athymic mice, grew progressively in intact mice given systemic c. parvum, though their growth was inhibited in similarly treated athymic mic ...	1977	299893
the adjuvant effect of corynebacterium parvum: t-cell dependence of macrophage activation.	splenic and peritoneal macrophages from mice treated with corynebacterium parvum enhanced the antibody response in vitro of normal nonadherent spleen cells to srbc, but not to dnp-pol. this enhancement was dependent on the dose and time of administration of c. parvum and could be abrogated by pretreatment with carrageenan. macrophages from t-cell-depleted mice failed to enhance the response, but this ability was restored if the mice had been reconstituted with purified t lymphocytes. macrophages ...	1977	299769
[initial experiences with a nation-wide austrian study of adjuvant chemo- and immunotherapy of colorectal cancer following radical surgery].	the outline of a cooperative study for adjuvant chemo-immunotherapy on radically operated colon-rectum-carcinoma, is presented. one group of patients receiving placebo, has to be randomized against another group who postoperatively received chemo-immunotherapy consisting of 5-fluorouracil and ccnu and corynebacterium parvum, which was administered intermittently, throughout one year. patients with rectum-carcinoma additionally receive radio-therapy with 1500 rad hd before surgery and 4500--5000 ...	1979	299221
effect of corynebacterium parvum and chronic toxoplasma infection on metastatic brain tumors in mice.		1979	291750
effect of host immune capability on radiocurability and subsequent transplantability of a murine fibrosarcoma.		1979	291749
bacteriocin (acnecin) activity of oral propionibacterium acnes.		1978	291496
an evaluation of corynebacterium parvum during remission maintenance therapy in pediatric patients with acute lymphoblastic leukemia.	the biologic impact and clinical toxicity of corynebacterium parvum administered at a dose of 5 mg/m2 by intravenous or subcutaneous routes were evaluated in 18 children receiving combination chemotherapy for maintenance of acute lymphoblastic leukemia (all) in remission. several nonspecific immunologic and hematologic parameters were evaluated. patients were also monitored for changes in cutaneous sensitivity to histamine. no changes in any parameter were observed in patients after only one cou ...	1979	287855
further observations on the inhibition of tumor growth by corynebacterium parvum with cyclophosphamide. x. effect of treatment on tumor cell kinetics in mice.		1979	286125
radiation-induced osteogenic sarcoma of c3h mouse: effects of corynebacterium parvum and wbi on its natural history and response to irradiation.		1979	285860
further observations on the inhibition of tumor growth by corynebacterium parvum with cyclophosphamide. ix. macrophage content of tumors in mice.		1979	285276
in vivo antitumor activity of various forms of delayed-type hypersensitivity in mice.	relationships among various forms of delayed-type hypersensitivity (dth) and nonspecific resistance to lewis lung tumor were studied in syngeneic and semisyngeneic mice. only the tuberculin type of dth obviated a virulent inoculum of 10(6) tumor cells. the jones-mote type of dth, even modified by cyclophosphamide pretreatment, produced a significant local inflammatory reaction which was unable to destroy tumor cells. the antitumor effect of the tuberculin type was observed in bcg-or in corynbact ...	1979	283275
tumor growth inhibition and potentiation of immunotherapy by indomethacin in mice.	indomethacin was continuously administered in the drinking water of inbred c3h mice given grafts of syngeneic 3-methylcholanthrene-induced fibrosarcomas. a minor proportion of these animals died at the same time as the untreated controls, and others completely rejected their tumors; however, in most cases, the tumor growth rate was significantly slowed, and growth recommenced rapidly after drug withdrawal. this was the pattern for tumors either in their 10th to 14th transplant generation or only ...	1979	281566
propionibacterium acnes-mediated humoral immune responses to tumor-specific antigens on rat liver cells transformed in vitro by chemical carcinogens.	effects of propionibacterium acnes on production of antibodies against tumor-specific membrane antigens were investigated in syngeneic inbred bd iv and bd vi rats. bd rat liver cell lines transformed in vitro by chemical carcinogens were used as target cells for tumor-specific antigens. by membrane immunofluorescence, antibodies against these rat liver cell lines were detected in syngeneic bd rat sera. antibodies were produced in syngeneic rats under the adjuvant effect of heat-killed p. acnes o ...	1978	281560
effect of corynebacterium parvum on tumor growth in the central nervous system of mice.		1978	278860
tumor immunology of experimental osteosarcoma.	a spontaneous ap positive c3h murine os was used to determine the effects of various treatment modalities. ap served as a useful circulating biomarker of the in vivo tumor growth. in animals whose tumor was amputated, the elevation of the marker indicated the presence of pulmonary metastases. it was used to establish the time of recurrence after a partially effective chemotherapy or combination modality. in this model, when the neoplasm was surgically excised at 10 days posttransplantation, 70-- ...	1978	278687
liver abscess in normal children with fever of unknown origin.		1978	263854
[histopathological aspects and macrophage reactivity in rats with guerin's t8 viral tumor after treatment with corynebacterium parvum].		1979	262125
the localization and distribution of gram-positive cocci in normal skin and in lesions of acne vulgaris.	the localization of gram-positive cocci in the normal skin and in the lesions of acne vulgaris was investigated using fluorescein-labeled antiserum raised to gram-positive, coagulase-negative cocci. the cocci were found in 10 of 19 specimens from normal facial skin and in 3 of 11 specimens from the normal skin of the rest of the body. the bacteria were found mostly in the openings of follicles, but in 6 of 10 facial skin specimens, they were also present deeply in the lumina of the dilated sebac ...	1975	239073
analysis of lipid composition of isolated human sebaceous gland homogenates after incubation with cutaneous bacteria. thin-layer chromatography.	the effects of specific species of skin bacteria on human sebaceous gland lipids in vitro were analyzed. isolated dissected sebaceous glands were pooled, homogenized, and sterilized, then incorporated into peptone-yeast extract medium and used as substrate for growth of propionibacterium acnes, p. granulosum, and staphylococcus epidermidis subgroup ii. the sebaceous lipids were analyzed by thin-layer chromatography before and after bacterial growth. the most striking effect of bacteria on sebace ...	1975	237966
quantitative microbiology of the scalp in non-dandruff, dandruff, and seborrheic dermatitis.	the composition of the scalp microflora was assessed quantitatively in normal individuals and in patients with dandruff and seborrheic dermatitis, disorders characterized by increasing scaling. three organisms were constantly found: (1) pityrosporum, (2) aerobic cocci, and (3) corynebacterium acnes. pityrosporum (mainly pityrosporum ovale) made up 46% of the total microflora in normals, 74% in dandruff, and 83% in seborvheic dermatitis. the geometric mean number of organisms per cm-2 in non-dand ...	1975	237965
[oral antigen treatment (oral vaccination) in acne diseases].		1975	236624
effect of corynebacterium acnes on interferon production in mouse peritoneal exudate cells.	corynebacterium acnes, an organism closely related to c. parvum, has been recognized to have a striking effect on the reticuloendothelial system, as well as on both humoral and cellular immunity. in mice previously exposed to c. acnes, serum interferon levels induced by injection of newcastle disease virus (ndv), chikungunya virus (cv), and polyinosinic-polycytidylic acid are suppressed. when peritoneal macrophages and lymphocytes from animals exposed to c. acnes were cultivated in vitro, their ...	1975	234914
[hematological observations in patients following immunostimulation through intrapleural application of corynebacterium parvum].	the ludwig lung cancer study group aims to investigate the role of immunotherapy as adjuvant treatment modality in operable non-small cell bronchial carcinoma. the participants are 12 european clinics and institutes. with a proven accrual of 350 patients per year the group offers a sharp tool in clinical oncology with regards to bronchial carcinoma. the accrual phase of the first trial was closed on february 2, 1979 with 475 patients, starting a new protocol on february 5, 1979. the ongoing rand ...	1979	233458
[effect of silica on the anti-tumour activity of "corynebacterium parvum" (author's transl)].	silica, a specifically toxic substance for macrophages, has been used to study the role of these cells in the corynebacterium parvum-induced anti-tumour protection. in the two experimental tumours studied, silica, c. parvum and tumour cells were injected by the same route: the intravenous route in the case of lymphosarcoma in xvii mice and the intraperitoneal in that of mammary carcinoma in c3h mice. the inhibition of the anti-tumour effect of c. parvum by the administration of silica was more p ...	1979	232975
functional similarity and diversity in peritoneal macrophage populations induced in vivo by various stimuli.		1979	232817
nor-mdp, saponin, corynebacteria, and pertussis organisms as immunological adjuvants in experimental malaria vaccination of macaques.	vaccination of primates against malaria using antigen derived from erythrocytic parasite stages has been most successful where freund's complete adjuvant has been employed. since this adjuvant is clinically unacceptable its replacement is a matter of urgency.in the present work a muramyldipeptide derivative (nor-mdp) given in mineral oil has proved to be partially effective as an adjuvant for merozoite vaccination of macaca mulatta against plasmodium knowlesi, and saponin has proved to be effect ...	1979	232428
immunotherapy of lung cancer. ii. review of clinical trials in small cell carcinoma.		1979	226262
antitumor activity and lymphoreticular stimulation properties of fractions isolated from corynebacterium parvum.		1979	225023
generation of cytotoxic lymphocytes against human tumor cells in vitro by various soluble microbial extracts.		1979	222929
nicotinamide adenine dinucleotide-splitting enzyme in normal, elicited and activated peritoneal macrophages of the mouse.		1979	222905
chemical properties of the principle in c. parvum that produces splenomegaly in mice.	suspensions of wellcome c. parvum strain 6134 produce splenomegaly in mice when injected i.p. in amounts as low as 20 microgram. this lymphoreticular stimulatory activity is extremely sensitive to cell breakage and is abolished by heating for 4 h at 100 degrees. periodate oxidation of the bacteria destroys their capacity to produce splenomegaly and abrogates the agglutination of intact c. parvum by con a. mild hcl hydrolysis also abolished the splenomegaly but phenol:chloroform:ether and chlorof ...	1979	220183
low trimethoprim susceptibility of anaerobic bacteria due to insensitive dihydrofolate reductases.	all the 28 bacteroides fragilis strains investigated were susceptible to sulfamethoxazole (minimal inhibitory concentration < 16 mug/ml) and resistant to trimethoprim (tmp; minimal inhibitory concentration > 4 mug/ml). synergism between sulfamethoxazole and tmp was present in all strains at a ratio of 1:1. the few clostridia investigated proved more resistant to both compounds. dihydrofolate reductases from b. fragilis, c. perfringens, and some other anaerobic species were isolated. inhibition p ...	1979	218496
prostaglandin-like substances in propionibacterium acnes ii. stimulatory effect on ovarian cyclic amp.	the prostaglandin-like substances (pls) from propionibacterium acnes increased the ovarian tissue levels of cyclic amp (camp) approximately 2-fold. the lipid material extracted from p. acnes thus behaved like pg's of the e-type, and since it is unlikely that other known stimulators of the ovarian camp system can be present in the bacterial lipid fraction, these experiments give further evidence in favour of the occurrence of pls in p. acnes.	1979	217719
effects of corynebacterium parvum on murine myeloid leukaemia.	the effects of c. parvum on rfm/un myeloid leukaemia were studied. mice inoculated with 7.0 mg but not 0.7 mg c. parvum i.p. survived significantly longer than untreated leukaemic mice (p less than 0.001). administration of silica abrogated the effects of c. parvum, whilst polyvinyl pyridine-n-oxide prevented the inhibitory effects of silica. these studies demonstrate that a single large dose of c. parvum, either before or after leukaemic-cell passage, can significantly prolong the survival of r ...	1978	217398
corynebacterium parvum as an adjuvant for trypanosoma cruzi epimastigote vaccines: a comparison with saponin and bordetella pertussis.	the effect was compared in cba mice of adding corynebacterium parvum, saponin, and bordetella pertussis to living or killed trypanosoma cruzi (y strain) epimastigote vaccines on the induction of protective immunity against subcutaneous (s.c.) challenge with blood trypomastigotes. the addition of c. parvum to a low dose of t. cruzi vaccine, which alone was non-protective, generated a greater degree of protection than did saponin or b. pertussis. c. parvum alone increased resistance to infection t ...	1979	216967
effect of silver nitrate application on the conjunctival flora of the newborn: and the occurrence of clostridial conjunctivitis.	newborn conjunctival cultures were obtained from 35 babies prior to silver nitrate application and 48 hours later. on initial culture, 46 facultative bacteria and 27 anaerobes were recovered; 48 facultative and 18 anaerobes were recovered after 48 hours. haemophilus vaginalis, bacteroides species and anaerobic cocci decreased in numbers, whereas s. epidermidis, micrococcus and propionibacterium acnes increased during this time interval. clostridial species were isolated from two cases who develo ...	1978	216788
peritoneal macrophage activation indicated by enhanced chemiluminescence.	a number of studies have demonstrated the ability of various bacterial preparations, protozoa, and chemicals to activate macrophages and concomitantly to enhance host resistance to both tumors and infections. recently, viral infections have been shown to have a similar effect upon macrophage function. to better define the metabolic state of activated macrophages, we have evaluated the ability of peritoneal cells (pc) from vaccinia virus- or murine cytomegalovirus-infected or corynebacterium parv ...	1978	213391
defective in vitro lymphoproliferative responses to herpes simplex virus in patients with frequently recurring herpes infections during the disease-free interval.		1978	213220
protection of mice against viral infection by corynebacterium parvum and bordetella pertussis.	mice could be significantly protected against infection with herpes simplex virus (hsv) by i.p. or i.v. injection of killed corynebacterium parvum 7 days before infection. this protection was seen in inbred strains of mice with a different degree of sensitivity to hsv and after both i.p. and i.v. infection. resistant mice immunosuppressed by x-irradiation and showing an increased susceptibility to hsv could also be protected by a previous injection of c. parvum. elevated levels of interferon wer ...	1978	212522
synergistic lysis of erythrocytes by propionibacterium acnes.	sheep and human erythrocytes, partially processed by staphylococcus aureus or clostridium perfringens, were susceptible to lysis in the presence of propionibacterium acnes. p. acnes liberated a lipase that was detected on tween 80 agar and also on phospholipase c-precipitated egg yolk agar. such a lipase might have contributed in the process of an intensified cellular lysis. similar reactions were attempted with lactobacillus acidophilus, known to possess a nondiffusible lipase, and failed to pr ...	1978	212450
treatment of an intracerebral rat brain tumor with corynebacterium parvum and radiation.	rats bearing intracranial brain tumors were treated with single ip injections of the killed corynebacterium parvum, a single dose of x-rays, or a combination of both treatments. animals given injections of 2.6 mg c. parvum 12 days after implantation of the tumor had a median survival time (mst) of 50 days compared with an mst of 44 days for an untreated group. these animals given c. parvum had a 33% increased life-span (ils) that was significant at the p=0.05 level. although the mst of animals t ...	1977	206836
enhanced resistance against junin virus infection induced by corynebacterium parvum.	the effects of intraperitoneal administration of corynebacterium parvum on the course of junin virus infection in mice were investigated. this treatment produced enhanced resistance to the virus infection, evidenced by an increase in both survival times and the proportion of survivors. the protective effect was dependent upon the dose of c. parvum, and 280 mug/g of body weight was found to be the optimal dose. in various experiments, about 80% of the infected animals receiving this dose survived ...	1978	205508
binding of microorganisms to the macrophage plasma membrane; effects of enzymes and periodate.	the nature of the binding of c. parvum organisms to the surface of glass-adherent mouse peritoneal exudate cells in vitro was studied using pretreatment of the cells with various enzymes and periodate. trypsin, pronase, beta-galactosidase, phospholipases a, c and d and periodate all caused a decrease in binding to 40-60% of untreated control. neuraminidase led to a 30% increase in binding. the binding ability returned to normal after 1 h at 37 degrees in culture medium following exposure to all ...	1978	205234
combined use of local irradiation and corynebacterium parvum in the treatment of the murine line 1 lung carcinoma.		1978	204955
mechanism of action of c. parvum on a solid, subcutaneous mouse tumour.	the effects of i.v. c. parvum on the growth of a.s.c. inoculum of the lewis lung carcinoma were studied in normal c57 b1 mice, and in those in which separate components of the immune response were impaired. c. parvum given either at the same time as tumour inoculation or when the carcinoma was 1 cm in diameter, and fully vascularised, reduced tumour growth. macrophages were impaired by silica (si), cortisone acetate (ca) or trypan blue (tb), and t cells by thymectomy and sublethal irradiation (t ...	1977	204533
detection of alcohols and volatile fatty acids by head-space gas chromatography in identification of anaerobic bacteria.	a head-space gas chromatographic technique for the analysis of volatile bacterial metabolites is described. bacteroides fragilis, clostridium perfringens, and propionibacterium acnes, cultured in a glucose-containing peptone yeast extract medium, were studied. the head-space technique was compared with the injection of the complete liquid culture medium, and solvent extracts thereof, into the gas chromatograph. volatile fatty acids could be detected by all three methods, whereas alcohols produce ...	1978	203602
possible involvement of natural killer cells in bone marrow graft rejection.	in the present study comparison between natural killer (nk) cells and cells responsible for rejection of bone marrow grafts was made. both cell populations were found to be inhibited by cyclophosphamide, silica, carrageenan, and c. parvum. the reactivity of both cell populations occurred late in life and was not expressed in infant mice. mice tolerant to bone marrow grafts and, therefore, accepting parental marrow transplants showed also decreased nk cell reactivities. these common features betw ...	1977	203349
enzymatic and hemolytic properties of propionibacterium acnes and related bacteria.	the production of chondroitin sulfatase, hyaluronidase, deoxyribonuclease, gelatinase, phosphatase, lecithinase, and hemolysins was examined in 95 strains of propionibacterium acnes and four related species of anaerobic, respectively, microaerophilic coryneform bacteria (p. avidum, p. lymphophilum, p. granulosum, and corynebacterium minutissimum). all enzymes could be demonstrated in at least one representative of the species tested. those propionibacterium species most frequently found in acne ...	1977	201661
abrogation of antitumor effects of corynebacterium parvum and bcg by antimacrophage agents: brief communication.	the consistently demonstrable antitumor effect of corynebacterium parvum and bcg against a 7,12-dimethylbenz[a]anthracene-induced rat fibrosarcoma, growing either as a localized subcutaneous tumor or in ascites form, was abrogated by treatment of rats with antimacrophage agents such as silica or carrageenan.	1977	200763
antibodies to bacterial and tumor-derived antigens in sera from normal guinea pigs.	antibodies that react with radiolabeled antigens derived from guinea pig line-10 tumor cells and mycobacterium bovis (bcg) were detected in sera from normal tumor-free strain-2 guinea pigs (ngps). binding by ngps to the two antigens was inhibited by extracts of either line-10 cells or bcg. binding by ngps to the line-10 antigen was inhibited by a number of other bacterial extracts. ngps was tested after absorption with a variety of cells including line-10, line-1, normal guinea pig spleen, norma ...	1977	197169
protection against herpes simplex virus infection in mice by corynebacterium parvum.	corynebacterium parvum administered in mice prior to herpes simplex virus (hsv) infection significantly protected them against lethal encephalitis. this was seen both with a mouse strain highly susceptible to hsv and with one relatively resistant to hsv. mice immunosuppressed by cyclophosphamide and showing an increased mortality after hsv infection were also protected by c. parvum pretreatment. however, c. parvum given simultaneously with or after hsv infection did not exert a therapeutic effec ...	1977	194841
immunological mechanisms in metastatic spread and the antimetastatic effects of c. parvum.	the effects of the host's immune response on metastatic spread was investigated by observing the numbers of pulmonary metastases that developed from an s.c. implant of the lewis lung carcinoma in c57bl mice in which different cell populations had been suppressed. macrophage function was impaired by treatment with silica (si), cortisone acetate (ca), or trypan blue (tb). t-cell function was depressed by adult thymectomy and sublethal irradiation, or by treatment with antilymphocyte serum (als). m ...	1977	193547
immunomodulator-induced resistance against herpes simplex virus.		1977	193122
immunomodulation of host resistance to experimental viral infections in mice: effects of corynebacterium acnes, corynebacterium parvum, and bacille calmette-guérin.	resistance to a representative group of experimental virual infections in mice was significantly enhanced by nonspecific modulation of host defense mechanisms. corynebacterium acnes, corynebacterium parvum, and bacille calmette-guérin were effective in enhancing host resistance. animals treated seven to 10 days before inoculation of virus were protected against a lethal infection with herpesvirus hominis type 2, encephalomyocarditis virus, murine cytomegalovirus, or semliki forest virus. the pro ...	1977	192811
modulation of mouse anti-trinitrophenyl plaque-forming cell affinity by adjuvants or lectins.	the efffects of several kinds of adjuvants or lectins, such as corynebacterium parvum, dextran, poly au, poly ic, dibutyryl camp, concanavalin a (con a), phytohemagglutinin (pha) and pokeweed mitogen (pwm) on anti-trinitrophenyl (tnp) direct plaque-forming cells (pfc) in the spleen of mice and the affinity of antibodies produced by these pfc were examined. the numbers of anti-tnp pfc in the spleens of mice which had been injected with c. parvum 7 days in advance were greater than those in contro ...	1976	190432
clinical immunoadjuvant studies with tilorone, deaa fluorene (rmi 11,002da), and corynebacterium parvum and some observations on the role of host resistance and herpes-like lesions in tumor growth.		1976	187101
immunochemotherapy with corynebacterium parvum in disseminated cancer.		1976	187100
immune response against hamster erythrocytes in the low-responder mouse strains. xi. strain difference in the effects of various microbial adjuvants.	enhancing and suppressing effects of microbial adjuvants were studied in female mice of the c3h/he, akr and sl strains. propionibacterium acnes, bordetella pertussis, bcg and yeast cell wall (ycw) were chosen as adjuvants. as antigens, we chose hamster erythrocytes (hrbc) which proved to be a weak antigen for mice. adjuvants were given on day --7, day 0 or day 3, and hrbc were injected on day 0. the results were as follows. 1) p. acnes facilitated igm and igg antibody production in akr mice and ...	1976	186656
c. parvum suppression of rat tumours in athymic nude mice.		1976	184813
[treatment of acne vulgaris in practice].	the aim of all acne therapy is sebostasis and treatment of corynebacterium acnes as well as secondary infected postular changes. after discussion of tretinoin (retinoic acid)-treatment it is reported about 35 cases of acne vulgaris which were treated with the combination of hexachlorophenspiritus 1%,silicatgel locally and tetracycline orally. the good results of this treatment are reported. side effects have not been observed.	1976	184016
hydrosoluble immunostimulants of bacterial and synthetic origins.	the role of whole mycobacteria, mycobacterial cell walls and waxes d as immunostimulants was well established many years ago. more recently three different research groups have shown that hydrosoluble components from mycobacterial and other bacterial origins were as active as waxes d or cell walls and were free of many side-effects. studies concerning the relationship between structure and activity were achieved which led to the description of a small biologically active fragment and to a first ...	1976	181265
effect of pretreatment with mycobacterium bovis (strain bcg) and immune syphilitic serum on rabbit resistance to treponema pallidum.	stimulation of the rabbit reticuloendothelial system with viable mycobacterium bovis (strain bcg), and other agents, had no effect on the development of syphilitic lesions after intradermal or intravenous inoculation with graded doses of treponema pallidum (virulent nichol's strain; mean infective doses less than 10). the simultaneous administration of immune syphilitic rabbit serum retarded the development of lesions, but this appeared to be due solely to the immune serum, suggesting no synergi ...	1975	172450
suppression of cell-mediated tumour immunity by corynebacterium parvum.		1975	170531
histological and combined chemoimmunostimulation therapy studies against a murine leukemia.	a graffi murine leukemia was utilized as a model system to investigate the effect of chemoimmunostimulation therapy. subcutaneous inoculation of approximately 1.0 times 10(6) tumor cells resulted in a rapidly growing tumor at the site of inoculation and subsequent development of splenomegaly and lymphoadenopathy. all animals succumbed to the leukemia within 24 to 30 days. treatment of diseased animals with two courses of cytoxan over a 2-week period resulted in a remission period of approximatel ...	1975	170212
[first clinical trials of treatment of primary liver cancer with immunostimulating agents administered by systemic or intratumoral route].		1975	170046
acne.	the cause of acne is still obscure, but genetic predisposition, sebaceous overactivity, overgrowth of bacterial flora and exposure to comedogenic substances are all significant factors. acne lesions occur mainly in sebaceous follicles, which are characterized by deep follicular canals and large sebaceous glands. the associated seborrhea is not due to a circulatory excess of androgens but may be caused by a local amplification of androgenic activity. this, in turn, may be due to large numbers of ...	1979	161830
comparison of natural killer cells induced by kunjin virus and corynebacterium parvum with those occurring naturally in nude mice.	natural killer (nk) cells are rapidly elicited in the spleen and peritoneal cavity of mice inoculated intravenously or intraperitoneally with live kunjin virus, and more slowly in the peritoneal cavity of mice inoculated intraperitoneally with formalin-inactivated corynebacterium parvum. nk cells induced by either agent display cytotoxicity for a similar spectrum of syngeneic, allogeneic, and xenogeneic cultured cell lines. by contrast, the cells occurring naturally in the spleen of congenitally ...	1979	160889
extrusion of peritoneal catheter through abdominal incision: report of a rare complication of ventriculoperitoneal shunt.	a rare complication of a ventriculoperitoneal shunt is reported. the peritoneal catheter extruded through the well-healed abdominal incision. this is the first report of such an occurrence in an adult patient.	1979	160511
bacterial flora of comedones.	open comedones from thirty-eight patients with acne vulgaris on the face or back were compared for microbial flora. a total of eighty-three comedones from the face and sixty-three from the upper back were individually processed for quantitative bacterial analysis. the greatest difference between the flora of comedones at these two sites was that 44.6% of comedones from the back (compared to 9.6% from the face) harboured no aerobic cocci. the decreased prevalence of staphylococci in the lesions f ...	1979	160242
complement activation in acne vulgaris: consumption of complement by comedones.	comedones, the contents of acne lesions, were shown to consume scomplement hemolytic activity in normal serum. this consumption was stimulated by the addition of serum from patients with inflammatory acne. absorption of acne serum with propionibacterium acnes cells removed all stimulating activity. immunoelectrophoretic analysis of serum incubated with comedones revealed the conversion of c3 and factor b in normal serum. the addition of acne serum resulted in cleavage of c4. in serum treated wit ...	1979	159261