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effect of beta-lactam prodrugs on human intestinal microflora.the ampicillin prodrugs bacampicillin, pivampicillin, and talampicillin, the mecillinam prodrug pivmecillinam and the sulbactam prodrug sulbactam pivoxil all have a greatly improved oral availability compared to the parent drug. they show no antibacterial activity themselves until transformed into active drugs after absorption. this double advantage makes them less likely to influence the intestinal microbial ecosystem. ampicillin has been reported to cause marked changes in the colon microflora ...19863547627
intestinal infections with aeromonas. 19873544999
relationship of toxin production to species in the genus aeromonas.ninety-five strains of aeromonas were divided into three species--a. sobria, a. hydrophila and a. caviae--on the basis of results in 13 biochemical tests. the minimum number of tests necessary to distinguish these species was determined. culture filtrate of the strains were tested for cytotoxin and cytotonin, haemolysin and protease. one filtrate with high-titre cytotoxin, haemolysin and protease activities was subjected to gel filtration on sephadex g75 and isoelectric focussing. of the five ce ...19863540304
aeromonas hydrophila peritonitis in a renal transplant recipient. 19863540131
prostacyclin and thromboxane a2 in septic shock: species differences.prostacyclin and thromboxane a2 have been implicated as mediators of septic shock. correlations between the human prostanoid response to sepsis and experimental paradigms are poorly understood. the purpose of this study was to compare changes in plasma levels of prostaglandin 6-keto-f1 alpha (pgi) and thromboxane b2 (txb) during septic shock in sprague-dawley rats, domestic pigs, mongrel dogs, and man. severe sepsis followed by septic shock (systolic bp less than 90 mmhg) was induced in rats by ...19863539388
[treatment of neurosurgical bacterial meningitis using the combination of ceftriaxone-fosfomycin].16 patients with bacterial meningitis following a neurosurgical procedure were given a combination of ceftriaxone and fosfomycin. 8 microorganism were isolated: 2 staphylococcus epidermidis, 1 staphylococcus aureus, 1 neisseria meningitidis, 1 streptococcus pneumoniae, 1 haemophilus influenzae, 1 serratia marcescens and 1 aeromonas hydrophila. no pathogen was identified in the remaining cases. all of the isolated strains were susceptible to both antibiotics. in vitro, neither synergy nor antagon ...19863534726
thromboxane a2 mediates hemodynamic and respiratory dysfunction in graded bacteremia.thromboxane a2 has been implicated as a mediator of cardiorespiratory dysfunction in sepsis. this study evaluated whether or not thromboxane a2 was necessary or sufficient for these adverse effects to occur during bacteremia. fourteen adult swine under barbiturate anesthesia and breathing room air were monitored with arterial and pulmonary artery catheters. animals were studied for 4 hours in three groups: group i, graded infusion of 10(9)/ml aeromonas hydrophila; group ii, aeromonas hydrophila ...19863526603
microbial filtrates activate granulocytes without complement or prostaglandins.cardiorespiratory dysfunction in sepsis may be mediated by circulating complement, activated leukocytes, prostaglandins, or by a direct effect of endotoxin. the purposes of this study were to determine if pathogenic microbes produce these substances and to evaluate the direct effects of substances released by micro-organisms on granulocyte aggregation (ga). escherichia coli, (e. coli), aeromonas hydrophila (aeromonas h.), staphylococcus aureus (s. aureus), and candida albicans, (candida a.) were ...19863524892
multiple low-level antibiotic resistance in aeromonas salmonicida.mutants with multiple low-level antibiotic resistance were isolated from virulent wild-type aeromonas salmonicida strains exposed to a low concentration of any one of several low-molecular-mass (approximately 635 daltons or less) antibiotics. multiple resistance was toward beta-lactam compounds (penicillin g, ampicillin, cloxacillin), quinolones (flumequine, oxolinic acid, nalidixic acid), tetracyclines, chloramphenicol, and novobiocin. susceptibilities of the mutants toward several higher-molec ...19863524440
etiology and epidemiology of travelers' diarrhea in asia.travelers' diarrhea in asia has been studied among peace corps volunteers in thailand, japanese travelers, foreign residents in bangladesh, guests in hotels, and members of various tour groups. rates of diarrheal attack of greater than 50% during four- to six-week sojourns were reported for these groups. among travelers with diarrhea, the most commonly isolated pathogen was enterotoxigenic escherichia coli (20%-34%), followed by salmonella (11%-15%), shigella (4%-7%), campylobacter (2%-5%), and ...19863523709
cloning, expression, and mapping of the aeromonas hydrophila aerolysin gene determinant in escherichia coli k-12.dna sequences corresponding to the aerolysin gene (aer) of aeromonas hydrophila ah2 dna were identified by screening a cosmid gene library for hemolytic and cytotoxic activities. a plasmid containing a 5.8-kilobase ecori fragment of a. hydrophila dna was required for full expression of the hemolytic and cytotoxic phenotype in escherichia coli k-12. deletion analysis and transposon mutagenesis allowed us to localize the gene product to 1.4 kilobases of aeromonas dna and define flanking dna region ...19863522552
feasibility of same-day identification of members of the family vibrionaceae by the api 20e system.sixty isolates, comprising nine species of the family vibrionaceae, were tested with the api 20e 5-h same-day procedure (analytab products, plainview, n.y.). included were 27 aeromonas hydrophila isolates, 10 aeromonas sobria isolates, 7 aeromonas caviae isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolate. the 5-h profile numbers were specific fo ...19863517061
comparison of the quantum ii bacterial identification system and the automicrobic system for the identification of gram-negative bacilli.the quantum ii bacterial identification system (bid; abbott laboratories) is a microprocessor-based spectrophotometric system for identification within 4 to 5 h of both enteric and nonenteric gram-negative bacilli. we compared the bid with the automicrobic system (ams; vitek systems, inc.), using the most recent gram-negative identification card and software (ams-gni), for the identification of 501 clinical isolates of gram-negative bacilli, including 382 belonging to the enterobacteriaceae and ...19863517034
identification of enteric pathogens in the small and large intestine of children with diarrhea.enteric pathogens were identified in children with diarrhea from duodenal specimens obtained with a string capsule and from fecal specimens. rotavirus was identified in stools of 43 of 100 children, and was recovered from the small intestine from nine (21%) children who were excreting this virus. shigella was isolated from stools from 22, salmonella from 17, enterotoxigenic escherichia coli from eight, and aeromonas hydrophila from one of 100 children with diarrhea. in contrast to rotavirus, sal ...19863516549
bacterial diarrhoea.bacterial infections are important causes of diarrhoea in infants and children, particularly in developing countries and in other settings where standards of personal and community hygiene are low. knowledge of bacterial diarrhoeas has been significantly expanded in recent years by the finding that many episodes of acute diarrhoea are due to infections with bacteria which produce enterotoxins that interfere with intestinal fluid and electrolyte transport (the 'enterotoxigenic' diarrhoeas). sever ...19863514008
same day identification of gram-negative rods with the quantum ii microbiology system.the quantum ii microbiology system was challenged with 479 strains of enterobacteriaceae and 155 strains of nonfermenters (contained in the data base) and aeromonas/plesiomonas. the percentages of strains correctly diagnosed within 5h (i.e., with out additional tests) at greater than or equal to 80% probability were 78% and at greater than or equal to 50% probability, 84.7%.19863512962
a collaborative evaluation of a rapid, semi-automated identification system for gram-negative bacilli: the quantum ii bid.the recently-introduced semi-automated bacterial identification system, the quantum ii bid, is designed to identify fermentative and non-fermentative gram-negative bacilli in 4-5 h. the system was evaluated independently by the two participating laboratories. inter-laboratory reproducibility was determined by testing 181 strains in each laboratory and found to be 97.8%. a further 893 organisms, comprising 417 fresh clinical isolates and 476 stock cultures, were then tested by the system in the t ...19863512840
classical bacterial diarrhea: perspectives and update--salmonella, shigella, escherichia coli, aeromonas and plesiomonas.impressive advancements in our understanding of the mechanisms of diarrhea production and of the epidemiologic importance of these "classical" bacterial enteropathogens have been accomplished, but many areas are in need of further exploration. the recent development of gene probes and enzyme-linked immunosorbent assays for the identification of epec, etec and eiec will enhance immeasurably the ability to carry out large scale epidemiologic studies which are still needed to clarify the global imp ...19863511463
diarrhea associated with aeromonas hydrophila. 19863511459
stress factors that can affect studies of drug metabolism in fish.the effects of environmental stress conditions on the defense response of rainbow trout following a four week exposure to subacute levels of un-ionized ammonia or temperatures 5 c above and 5 c below the temperature optimum (15 c) were investigated. these experimental studies can serve as a model to evaluate the metabolic response of fish to external agents (e.g., drugs, vaccines) under environmental conditions seen in the culture of fish. blood and tissue immune parameters measured include hema ...19863509652
binding of clavulanic acid to the penicillin-binding proteins of aeromonas hydrophila. 19873509501
gram-negative septicemia in american alligators (alligator mississippiensis).six species of bacteria (family enterobacteriaceae) not commonly reported as associated with disease in american alligators (alligator mississippiensis) were documented, suggesting that aeromonas is not the only bacterium responsible for septicemia in crocodilians. these included citrobacter freundii, enterobacter agglomerans, proteus sp., morganella morganii, serratia marcescens, and klebsiella oxytoca. clinical signs of disease included intensive basking, anorexia, lethargy, flaccid limb paral ...19863503133
in vitro activity and beta-lactamase stability of a new penem, cgp 31608.the in vitro activity of cgp 31608, a new penem, against aerobic and anaerobic organisms was evaluated and compared with those of other beta-lactams. cgp 31608 inhibited escherichia coli, klebsiella pneumoniae, k. oxytoca, proteus mirabilis, citrobacter diversus, and salmonella, shigella, aeromonas, and yersinia spp. with mics for 50% of the strains (mic50s) of 2 to 4 micrograms/ml and mic90s of 4 micrograms/ml, compared with cefotaxime, ceftazidime, aztreonam, and imipenem mics of less than 0.2 ...19873496845
in vitro activity and beta-lactamase stability of two oral cephalosporins, ceftetrame (ro 19-5247) and cefetamet (ro 15-8074).ceftetrame (ro 19-5247) and cefetamet (ro 15-8074), two new orally administered aminothiazolyl imimomethoxy cephalosporins, inhibited hemolytic streptococci and streptococcus pneumoniae at less than or equal to 0.5 micrograms/ml but were less active against staphylococci than were cephalexin and cefaclor. they did not inhibit s. faecalis, s. faecium, listeria monocytogenes, corynebacterium jk species, or pseudomonas aeruginosa. haemophilus influenzae, branhamella catarrhalis, and neisseria gonor ...19863490827
synergism and antagonism in double beta-lactam antibiotic combinations.combinations of two beta-lactam antibiotics may be advantageous in certain clinical situations, providing a synergistic activity against specific organisms or a broad spectrum of antibiotic coverage. depending on the combination and the bacteria, synergism, indifference, or antagonism can be observed. synergism may occur when two beta-lactam antibiotics, acting on different penicillin-binding proteins, are combined or when a penicillinase-susceptible beta-lactam antibiotic is protected by anothe ...19863487247
beta-lactamases with high activity against imipenem and sch 34343 from aeromonas hydrophila.two groups of inducible beta-lactamases were found among nine isolates of aeromonas hydrophila. five isolates produced enzymes with high activity against the penem sch 34343 and the carbapenem imipenem: the enzymes from some of these isolates also had activity against penicillin, ampicillin, carbenicillin, cephaloridine and cephalexin but one isolate produced an enzyme with no detectable activity against cephalosporins. the other four isolates produced typical 'cephalosporinases' with activity a ...19863485091
cutaneous infections of the coast. 19873480441
in vitro activity of ci-934 compared with ciprofloxacin, enoxacin, norfloxacin, and vancomycin.the in vitro activity of the quinolone ci-934 was compared with ciprofloxacin, norfloxacin, enoxacin, and vancomycin against 607 gram-positive and -negative isolates. ci-934 inhibited 90% of the enterobacteriaceae, aeromonas hydrophila, and acinetobacter spp. at 1 microgram/ml. decreased activity was observed against pseudomonas aeruginosa. pseudomonas maltophilia, and other pseudomonas spp. with ci-934 mic90 greater than or equal to 8 micrograms/ml. ci-934 activity against gram-positive organis ...19873472704
antimicrobial activity of imipenem against 1386 clinical isolates.1386 isolates from clinical specimens were tested against imipenem by disc agar diffusion. the bacteria used in this study consisted of escherichia coli, enterobacter aerogenes, e. agglomerans, e. cloacae, klebsiella pneumoniae, k. oxytoca, k. ozanae, proteus mirabilis, p. vulgaris, providencia stuartii, p. rettgeri, acinetobacter calcoaceticus, citrobacter diversus, c. freundii, morganella morganii, serratia liquefaciens, s. marcescens, hafnia alvei, aeromonas hydrophila, pseudomonas aeruginosa ...19863468319
the comparative in-vitro activity of eight newer quinolones and nalidixic acid.the in-vitro antibacterial activity of nalidixic acid and the 4-quinolones, ciprofloxacin, norfloxacin, enoxacin, ofloxacin, pefloxacin, a-56619, a-56620 and ci-934 was assessed by determination of mics. the 4-quinolones were all highly active against most isolates of enterobacteriaceae, including nalidixic acid-resistant strains. ciprofloxacin (mics 0.002-2 mg/l) was the most active and a-56619 (mics 0.008-32 mg/l) was the least active. a-56619, a-56620, ofloxacin, ciprofloxacin and ci-934 were ...19863468100
the in-vitro activity of ci-934 compared with that of other new 4-quinolones and nalidixic acid.the in-vitro activity of ci-934, a new 4-quinolone compound, was compared with that of the other new 4-quinolones, enoxacin and ciprofloxacin, and also with that of nalidixic acid. ci-934 was more active than any of the other 4-quinolones tested against gram-positive aerobic organisms including staphylococcus aureus (mics 0.06-0.25 mg/l), beta-haemolytic streptococci (mics 0.12-0.5 mg/l), streptococcus pneumoniae (mics 0.25-0.5 ml/l), viridans streptococci (mics 0.06-0.5 mg/l) and most enterococ ...19863463557
activity of newer antimicrobial agents against aeromonas hydrophila. 19863463508
bacteremia due to aeromonas hydrophila: a report of 40 episodes. 19863461126
aeromonas infection following facial injury. 19863459840
[pulmonary abscess: aeromonas hydrophila caused by a complication of drowning in fresh water]. 19873450216
bacterial flora in bottled uncarbonated mineral drinking water.a quantitative study of bacterial populations in mineral water was carried out. samples were stored at 6 and 20 degrees c, and the colony counts were determined on tryptone agar plates incubated at 22 and 37 degrees c. samples were collected from the spring source in sterile glass flasks and from the bottling factory in conventional plastic and glass containers. in both cases, the initial population (10(1)-10(2) cfu/ml water) increased to 10(5)-10(6) cfu/ml after 3 days storage as determined fro ...19873446349
[geographic variations of the isolation of aeromonas hydrophila in infants with acute infantile diarrhea and a healthy population]. 19873444950
two-hour miniaturized system for detection of enteropathogenic bacteria in stool cultures.a miniaturized 2-h system for detection of lactose-negative potentially enteropathogenic bacteria on the basis of nine enzymatic tests was evaluated. the system was challenged with 210 strains of lactose-negative and lactose-positive species grown on macconkey agar. specific and constant patterns were found for salmonella, shigella a-c, yersinia enterocolitica, aeromonas spp. and vibrio cholerae. shigella sonnei and hafnia alvei had an identical pattern, likewise plesiomonas shigelloides, haloph ...19873440458
[spontaneous bacterial peritonitis caused by aeromonas hydrophila. presentation of 2 cases]. 19873438545
lymphadenopathy syndrome and hiv infection in multitransfused beta-thalassemia child.lymphoadenopathic syndrome (las), diagnosed also with histologycal studies, is described in a polytransfused 11-year old boy affected by beta-thalassemia major. after three days of the admission he suffered a serious acute diarrhea by aeromonas hydrophila, an opportunistic enteric bacteria demonstrated, up to date, in immunocompromised patients and never in aids patients. it is important to remark that beta-thalassemic patients are at high risk from hiv infections, either for the chronic need of ...19873435653
enteric pathogens in tropical aquaria.a total of 100 tropical aquaria from six pet shops were sampled for the presence of salmonella, campylobacter and aeromonas species. eight fish tanks contained unusual serotypes of salmonella. none had been used to house terrapins. one hundred and three isolates of aeromonas species were obtained from 98 tanks. one tank contained plesiomonas shigelloides. no campylobacter species were isolated. it is suggested that a tropical aquarium may constitute an infection hazard.19873428370
the effect of different brands of macconkey and xylose lysine deoxycholate agar on the isolation of aeromonas hydrophila.aeromonas hydrophila has been reported to be involved in the opportunistic infection of human beings, therefore, its rapid diagnosis from the clinical specimens was considered to be extremely important for the prognosis. the effect of different brands of macconkey (mac) and xylose lysine deoxycholate (xld) agars on the plating efficiency and colony size of a. hydrophila was evaluated by comparing with blood agar. results indicate that (i) except eiken's mac have low plating efficiency, all the m ...19873428016
[antibiotic resistance of several gram-negative bacteria from clinical and dissection material from the institute for poultry diseases of ludwig maximilian university, munich].a survey is given on antibiograms carried out with important bacterial strains isolated from birds during 1987. as compared with the results from earlier years no significant changes could be shown, the sensitivity ranging from 42.4% to 95.9%. the genera bordetella and aeromonas were included for the first time.19883420603
surface properties of autoagglutinating mesophilic aeromonads.the surface characteristics of 24 autoagglutinating (aa+) mesophilic aeromonads were investigated. one group of 16 was found to be highly related serologically by their reactive pattern against o antisera generated against three reference strains. subsequent characterization of 11 of these isolates (group 1) indicated that they had the following properties in common: precipitation after boiling (pab+), membership of serogroup o:11 (typing scheme of sakazaki and shimada), resistance to lysis by b ...19883417353
[frequency of aeromonas species in the stools of healthy and diarrheic patients].during two test periods (summer 1985 and january-august 1986) a total of 7713 stool specimens were examined for the occurrence of aeromonas spp. the isolation rate of the asymptomatic group was about 0.3%, the same rate was found in adults with diarrhea. in the stool specimens of 364 children 8 aeromonas spp. could be found. a. caviae was isolated in most cases of diarrhea. data obtained suggest that it seems to be useful to screen all diarrhoic stools of children for the occurrence of aeromonas ...19883402999
oxolinic acid in the trout: bioavailability and tissue residues.a study was performed on the serum bioavailability and tissue elimination of oxolinic acid in the trout. the compound was added to the diet and administered at a dosage-level of 12 mg/kg/day for 7 consecutive days. the study utilized an analytical technique, high performance liquid chromatography, which has been described in detail here. the results obtained demonstrate that serum concentrations higher than the mic for the control of the target pathogens (aeromonas and yersinia) can by sustained ...19883400972
aeromonas eucrenophila species nova aeromonas caviae a later and illegitimate synonym of aeromonas punctata.aeromonas caviae is a later and illegitimate synonym of aeromonas punctata. in order to secure continuity in literature, it is proposed to use the trivial term "caviae type" in connection with the species name a. punctata temporarily especially for strains isolated in connection to diarrhoea in children. aeromonas eucrenophila sp. nov. is described, type strain in ncmb 74. the new aerogenic psychrotrophic aeromonas species is separated genotypically from aeromonas hydrophila and aeromonas puncta ...19883394446
[analysis of degradative plasmids by pyrolysis gas liquid chromatography]. 19883388828
the effects of gamma irradiation on the lymphoid organs of rainbow trout and subsequent susceptibility to fish pathogens.fish were irradiated with 60co gamma rays at doses ranging from 10 to 50 gy. lethal doses were determined in fishes of different ages. for a given dose, fry and fingerlings were more susceptible than subadults. whatever the irradiation dose was, the fish displayed a sharp decrease in blood leucocyte count. at the lowest doses, this acute leucopaenia was reversible. the cellular damage in the lymphoid organs was particularly obvious in the thymus. the depletion of lymphoid cells from immunocompet ...19883388762
the phylogenetic origin of the bifunctional tyrosine-pathway protein in the enteric lineage of bacteria.because bifunctional enzymes are distinctive and highly conserved products of relatively infrequent gene-fusion events, they are particularly useful markers to identify clusters of organisms at different hierarchical levels of a phylogenetic tree. within the subdivision of gram-negative bacteria known as superfamily b, there are two distinctive types of tyrosine-pathway dehydrogenases: (1) a broad-specificity dehydrogenase (recently termed cyclohexadienyl dehydrogenase [cdh]) that can utilize ei ...19883386529
aeromonas hydrophila cellulitis. a case report. 19883385616
an unusual case of pyogenic meningitis due to aeromonas sobria. 19883382842
screening of bacteria with antiviral activity from fresh water salmonid hatcheries.bacteria isolated from two salmonid hatcheries were screened for antiviral activity against infectious hematopoietic necrosis virus (ihnv) to ascertain the presence of bacteria with anti-ihnv activity in the aquatic environment. out of 710 bacterial isolates from the water and sediment samples, 190 strains showed anti-ihnv activities of more than 50% plaque reduction. these antiviral activities were detected predominantly in pseudomonas, aeromonas/vibrio, and coryneforms. in one hatchery, the ba ...19883374405
preparation of metal-free enzymes. 19883374375
isolation of aeromonas salmonicida from masu salmon in the republic of korea.the isolation and identification of the gram-negative fish pathogen, aeromonas salmonicida salmonicida, from masu salmon (oncorhynchus masou) at the yang yang salmon hatchery in the republic of korea is described. the bacterium possessed certain characteristics in common with isolates from fish in japan. it is an important cause of mortality among cultured salmonids in the republic of korea. this is the first report of this fish pathogen from the asian mainland.19883373645
isolation and biochemical characterization of the s-layer protein from a pathogenic aeromonas hydrophila strain.the regular surface protein array (s layer) present on aeromonas hydrophila tf7 is composed of a single species of protein of apparent molecular weight 52,000. this protein was extracted from whole cells by treatment with 0.2 m glycine hydrochloride (ph 3.0). the protein was purified to homogeneity by ion-exchange chromatography and reverse-phase high-performance liquid chromatography. amino acid composition analysis showed that the protein contained 520 residues per molecule, 41% of which were ...19883372479
structure of an s layer on a pathogenic strain of aeromonas hydrophila.negative staining revealed a tetragonal surface array (s layer) on all the members of a serogroup of aeromonas hydrophila which possess high virulence for fish. the s layers were similar on all the strains examined, with unit cell dimensions of approximately 12 nm. a single representative strain, strain tf7, was selected for further analysis. freeze-cleaved and etched preparations and sections for electron microscopy showed that the s layer was the outermost component of the cell envelope. this ...19883372478
gas-liquid chromatographic analysis of fatty acid methyl esters of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae.clinical isolates of aeromonas hydrophila, a. sobria, and a. caviae whose fatty acid content had been analyzed by gas-liquid chromatography (glc) displayed the following qualitatively similar glc profiles: 12:0, 14:0, 15:0, 16:0, 17:0, 18:0, 16:1, 18:1, a-15:0, a-17:0, and 3-oh 14:0. the 16:0/17:0 area-percentage ratio separated the clinical aeromonads in accordance with their species designations. aeromonads treated with subminimal inhibitory concentrations of the antibiotic cerulenin displayed ...19883366862
[prevalence of aeromonas spp. pediatric gastroenteritis].a selective medium and biochemical tests were used to search for aeromonas spp. in the stools of 536 children, more than 90% of whom had "gastroenteritis", seen at sainte-justine hospital, montreal, in june or july 1986. the organism was found in seven children, six of whom had bloody diarrhea. one of the six, a 6 1/2-year-old boy, required intravenous alimentation and later manifested ulcerative colitis; the other five recovered. the remaining child, a 14-year-old boy, had synovitis of the knee ...19883355951
production of monoclonal antibody against aeromonas hydrophila haemolysin.two hybridoma cell lines that produce monoclonal antibodies against aeromonas hydrophila haemolysin were established by fusion of myeloma and spleen cells obtained from a mouse immunised with haemolysin detoxified with tetranitromethane. enzyme-linked immunosorbent assay (elisa) showed that the two purified monoclonal antibodies, b7 and b11, recognised the same epitope on the haemolysin molecule. antibody b7 neutralised the haemolytic and enterotoxic activities of the haemolysin. it is concluded ...19883346901
effect of incubation temperature on growth and soluble protein profiles of motile aeromonas strains.motile aeromonas spp. are said to have an optimal growth temperature of 28 degrees c. we performed growth studies on 24 isolates at 29 and 37 degrees c and found that the optimal growth temperature was not necessarily 28 degrees c. we also detected temperature-dependent differences in soluble protein production in some of these strains.19883343335
aeromonas hydrophila wound infection in a patient with cyclic neutropenia following a piranha bite. 19883340462
aeromonas-associated gastroenteritis in children.during a 20-month period 55 strains of aeromonas species were isolated from 53 children with diarrhea. the isolation rate of 2.5% for aeromonas compared with the rates of 4.5% for shigella, 3.3% for salmonella, 2.7% for campylobacter and 0.05% for yersinia. in 45 children aeromonas was the sole bacterial enteropathogen identified. aeromonas was also isolated from 2 (0.5%) of 380 asymptomatic children. despite its known lack of identifiable virulence properties, aeromonas caviae was the most prev ...19883340460
antimicrobials and fish: a review of drugs used to treat bacterial diseases of channel catfish and rainbow trout.the principal bacterial diseases of cultured rainbow trout (salmo gairdneri) and channel catfish (ictalurus punctatus), and the antibacterials used in their treatment are briefly reviewed. gram-negative bacteria predominate, and oxytetracycline is effective in treatment of infections induced by them. the only antibacterials now registered by the us food and drug administration are sulfamerazine for the treatment of furunculosis in trout, and oxytetracycline for the treatment of furunculosis in s ...19863334692
[a case of aeromonas hydrophila sepsis with generalized gas-formation and intravascular hemolysis]. 19873334425
phenotypic differentiation of members of the family vibrionaceae using miniaturized biochemical tests.enzymatic hydrolysis of 27 different chromogenic substrates and the assimilation of 44 carbon sources by 144 strains of vibrio species of clinical importance, aeromonas hydrophila and plesiomonas shigelloides were studied by standardized micromethods. some classical biochemical tests were also performed using the test kit tte-as (flow laboratories gmbh, meckenheim, frg). reading of results was done automatically by a photometer and test data were recorded and stored by a microcomputer. all speci ...19873326370
evaluation of a small "conventional" identification system for fermentative gramnegative rods using a computerized data base.a small "conventional" identification system consisting of six tubed media totalling eleven reactions was used to identify 467 strains of enterobacteriaceae, 15 strains of aeromonas sp., and 3 strains of plesiomonas shigelloides. the system identified 371 strains (76.5%) correctly to maximum identification (species or genus) level and an additional 13 strains (2.7%) to genus level where species level identification would have been possible. ninety strains (18.6%) were not identified. only 11 str ...19873326369
[fatal aeromonas septicemia in two patients with acute myelogenous leukemia: clinical significance in neutropenic states and review of the literature]. 19873323564
a comparison of the distribution of extracellular proteins produced by the protease-secreting organism aeromonas salmonicida during aerobic and anaerobic growth.aeromonas salmonicida was grown aerobically and anaerobically in supplemented 3% (w/v) tryptone soya broth medium for 24 h at 25 degrees c. although the bacterial density achieved was 4.9 times higher in the aerobic culture, the exoprotein produced per unit of bacterial dry weight was only 1.9 times higher than in the anaerobic culture. however, the protease activity of the exoprotein showed a marked reduction anaerobically, being only one-tenth of that of the exoprotein produced aerobically. th ...19863322167
does aeromonas hydrophila preferentially colonize the bowels of patients with hematologic malignancies?weekly cultures of stools from neutropenic patients and bone marrow transplant recipients yielded aeromonas hydrophila from 8% of 88 patients over a 2-yr period. during this time stools in the routine enteric laboratory yielded a. hydrophila in 0.24% of 1632 patients. although the patient groups and culture methods were not directly comparable, this significant difference in isolation rate (p less than 0.001) may reflect a higher colonization rate in the immunocompromised patient.19873319373
[aeromonas spp. as pathogens in human infections: diagnosis and clinical significance].aeromonads have been described as causative agents in a wide variety of infections in immunosuppressed as well as in immunocompetent patients. it is still controversial whether or not some or all three currently recognized species have to be considered as enteropathogenic. clinical and epidemiological data are incomplete and contradictory although laboratory diagnosis does not cause real problems.19873315971
[aeromonas hydrophila as the causative agent of ileocolitis]. 19873315547
[tte-ras (titertek-enterobac-ras), a new micromethod for the semi-automatic identification of enterobacteriaceae within 5 hours].titertek-enterobac-ras (tte-ras), a new semi-automated system for the identification of the enterobacteriaceae-within five hours, has been evaluated and compared with conventional methods. this note presents the results upon 655 strains, mainly enterobacteriaceae tte-ras provided correct identification for about 92 p. cent and 97 p. cent of enterobacteriaceae respectively before and after carrying out supplementary tests according to manufacturer's instruction. tte-ras gave 97 p. cent specific r ...19873314607
natural microbiologic hazards of the aquatic environment. 19873311341
marine bacteria which produce tetrodotoxin.a number of type strains of marine bacteria, including members of the family vibrionaceae, were cultured and examined for tetrodotoxin productivity by high-performance liquid chromatography and gas chromatography-mass spectrometry. most of the vibrionaceae strains produced tetrodotoxin, anhydrotetrodotoxin, or both.19873310884
marker exchange mutagenesis of the aerolysin determinant in aeromonas hydrophila demonstrates the role of aerolysin in a. hydrophila-associated systemic infections.we report here on the isolation of isogenic strains of aeromonas hydrophila ab3 deleted for a segment of the aerolysin gene. all aer mutants obtained lacked the 49-kilodalton aerolysin gene product and were neither hemolytic for blood erythrocytes nor cytotoxic for chinese hamster ovary tissue culture cells. one such mutant, ab3-5, was used in a mouse toxicity model to evaluate the role of aerolysin in the pathogenesis of a. hydrophila infections. the strain had a 50% lethal dose (ld50) of great ...19873305370
[study of the sensitivity of enteropathogenic bacteria to nifurzide].nifurzide, a synthetic antimicrobial agent of the nitrofuran group, was tested on 320 enteropathogenic bacteria. minimal inhibition concentrations (mic) (expressed in mg/l) were determined using the standard agar dilution technique. the mics were excellent for campylobacter spp. (97 strains), mic 50:0.5, mic 90:0.5; slightly higher for shigella spp. (50 strains) and aeromonas spp. (22 strains), mic 50:2, mic 90:4; and highest for salmonella spp. (100 strains) and yersinia enterocolitica (49 stra ...19873302859
in vitro activity and beta-lactamase stability of a new monobactam, b0-1165.b0-1165 is a 1-carboxy-1-cyclopropoxyamino,4-fluoromethyl monobactam. it inhibited the majority of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, citrobacter diversus, aeromonas hydrophila, proteus mirabilis, proteus vulgaris, providencia rettgeri, providencia stuartii, yersinia enterocolitica, haemophilus influenzae, neisseria gonorrhoeae, and salmonella and shigella species at less than or equal to 0.125 microgram/ml. overall, its in vitro activity was similar to that of aztreona ...19873300528
a comparative study of the formation of extracellular proteins by aeromonas salmonicida at two different temperatures.aeromonas salmonicida was grown in a supplemented 3% (w/v) tryptone soya broth medium at 10 degrees c, a temperature at the lower end of the range over which furunculosis has been observed to occur in the field, and 25 degrees c, the optimum temperature for growth. similar bacterial densities in the range 2.35 +/- 0.05 mg dry wt/ml were achieved in the two cultures at the beginning of the stationary phase of the growth cycle, after 125 h at 10 degrees c and 18 h at 25 degrees c. at this point, a ...19873298187
partial purification and characterization of an extracellular proteinase from aeromonas hydrophila strain a4.an extracellular metalloproteinase from aeromonas hydrophila strain a4, isolated from milk, was purified by a factor of 300 by chromatography on deae-cellulose and sephadex g-150. the enzyme had a mol. wt of 43,000 and contained 2 g atom ca/mol. it was active over a ph range 4.8-9.5 and had optimum activity on casein at ph 7.0 with km = 0.17 mm. it was strongly inactivated by metal chelators and the apoenzyme was fully reactivated with ca2+, mn2+ or co2+. heavy metal ions such as ag+, hg2+, fe2+ ...19883290294
specific inhibition of aspartase by s-2,3-dicarboxyaziridine.aspartase of escherichia coli was inhibited in a competitive manner by s-2,3-dicarboxyazirdine (dcaz), an antibacterial substance against aeromonas salmonesida. the inhibition constant (ki) was 55 microm, which was as low as less than one tenth that of the km value for the substrate, l-aspartate. in view of the fact that both aspartase and fumarase (j. greenhut et al. (1985) j. biol. chem. 260, 6684-6686) were inhibited by dcaz in competitive manners, common features of the reaction mechanism of ...19883289541
pneumonia due to aeromonas hydrophila-complex: epidemiologic, clinical, and microbiologic features.review of published data examining the various types of infections produced by strains of the aeromonas hydrophila-complex demonstrates a paucity of information on lower respiratory tract infections due to this organism. although it is rarely cited as a cause of pneumonitis, we have been able to collect epidemiologic, clinical, and microbiologic data on eight patients who have had evidence of aeromonas pneumonia. interestingly, seven of the eight patients were male, and the average age for the g ...19883282317
aeromonas hydrophila gas gangrene: a case report of management with surgery and hyperbaric oxygenation. 19883282228
molecular cloning of a phospholipid-cholesterol acyltransferase from aeromonas hydrophila. sequence homologies with lecithin-cholesterol acyltransferase and other lipases.we have determined the nucleotide sequence of a gene encoding aeromonas hydrophila phospholipid-cholesterol acyltransferase, an enzyme which shares many properties with mammalian lecithin:cholesterol acyltransferase. the derived amino acid sequence of the protein contains two regions which are homologous to the proposed active sites and binding sites of the plasma acyltransferase and to similar sequences in other interfacially acting lipolytic enzymes. the amino terminus is preceded by a typical ...19883280033
tigemonam, an oral monobactam.tigemonam is an orally administered monobactam. at less than or equal to 1 microgram/ml it inhibited the majority of strains of escherichia coli, klebsiella spp., enterobacter aerogenes, citrobacter diversus, proteus spp., providencia spp., aeromonas hydrophila, salmonella spp., shigella spp., serratia marcescens, and yersinia enterocolitica. at less than or equal to 0.25 microgram/ml it inhibited haemophilus spp., neisseria spp., and branhamella catarrhalis. it did not inhibit pseudomonas spp. ...19883279906
surface protein composition of aeromonas hydrophila strains virulent for fish: identification of a surface array protein.the surface protein composition of members of a serogroup of aeromonas hydrophila which exhibit high virulence for fish was examined. treatment of whole cells of representative strain a. hydrophila tf7 with 0.2 m glycine buffer (ph 4.0) resulted in the release of sheets of a tetragonal surface protein array. sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis analysis showed that this sheet material was composed primarily of a protein of apparent molecular weight 52,000 (52k protein) ...19883276660
the growth of aeromonas hydrophila k144 in ground pork at 5 degrees c.the influence of nacl, ph, atmosphere, and background microflora on the growth and/or survival of aeromonas hydrophila k144 was studied in ground pork held at 5 degrees c. in ground pork, a hydrophila was sensitive to ph values below 6.0 in the form of either a low starting ph in the pork itself or induced by lactic acid bacteria action on added glucose. growth of the organism is inhibited by nacl levels of 3% (w/w) (approx. 4% brine content). a hydrophila grew in vacuum-packaged ground pork; it ...19883275309
aeromonas hydrophila infections in children. 19883272526
characterization of an aeromonas hydrophila strain isolated on a septicemic out-break in a fish-farm of spain.an aeromonas hydrophila strain (ah-3) was isolated from a septicemic out-break on a gold-fish farm near barcelona (spain). on the bases of their virulence and surface characteristics was classified as moderate to weakly virulent.19883271064
proceedings of the second aeromonas/plesiomonas workshop. miami beach, florida, usa, 7 may 1988. 19883269365
gene probes for the detection of aerolysin in aeromonas spp. 19883269364
effect of recent antibacterial agents against bacteria causing diarrhoea.the susceptibility of 424 bacterial isolates causing diarrhoea were tested by agar dilution technique on mueller-hinton agar against amoxicillin, ampicillin, ceftriaxone, chloramphenicol, co-trimoxazole, ciprofloxacin, doxycycline, norfloxacin and ofloxacin. the bacterial species included were aeromonas hydrophila, edwardsiella tarda, pleisomonas shigelloides, salmonella spp., shigella spp., vibrio cholerae and vibrio parahaemolyticus. the most active compounds were the fluorinated 4-quinolones ...19883266900
beta-lactam resistance in aeromonas spp. caused by inducible beta-lactamases active against penicillins, cephalosporins, and carbapenems.use of cefoperazone in a patient with aeromonas caviae in the respiratory tract selected a mutant that constitutively produced beta-lactamase. this mutant, in contrast to its parental strain with an inducible beta-lactamase, showed enhanced resistance to newer cephalosporins and aztreonam. this observation suggested that species of aeromonas, like those of other genera with inducible beta-lactamases, may pose therapeutic problems associated with the rapid development of multiple beta-lactam resi ...19883264129
aeromonas hydrophila cellulitis and wound infections caused by waterborne organisms. 19883257483
aeromonas hydrophila septicaemia and muscle abscesses associated with immunosuppression.we report a case of septicaemia and muscle abscesses due to aeromonas hydrophila. the patient was immunosuppressed due to hodgkin's disease and treatment with cytotoxic chemotherapy. the abscesses resolved after prolonged antibiotic therapy. organisms that were once considered rarely pathogenic are emerging as increasingly important in causing potentially lethal infections in immunosuppressed patients.19883251226
three-dimensional structure of the crystalline surface layer from aeromonas hydrophila.the three-dimensional (3d) structure of the crystalline surface layer of aeromonas hydrophila has been determined to a resolution of 1.3 nm by crystallographic electron microscopy. the s-layer has tetragonal symmetry, with a unit cell dimension of 12.0 nm and a thickness of 5.6 nm. the 3d reconstruction reveals a distinct domain structure, with one main protein mass at one of the fourfold symmetry axes and a minor part at the other.19883249041
[an epidemic of food poisoning by aeromonas hydrophila]. 19883248456
[characteristics of immunoglobulins specific for lps and lipid a of aeromonas sobria and non 01 vibrio cholerae]. 19883246917
[serological cross reactions of lps and lipid a isolated from aeromonas sobria and non 01 vibrio cholerae]. 19883246916
[septicemia caused by aeromonas/plesiomonas in adults]. 19883244913
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